AR051478A1 - TRANSFORMATION AND SELECTION OF EUCALYPTUS UROPHYLLA - Google Patents

TRANSFORMATION AND SELECTION OF EUCALYPTUS UROPHYLLA

Info

Publication number
AR051478A1
AR051478A1 ARP050104647A ARP050104647A AR051478A1 AR 051478 A1 AR051478 A1 AR 051478A1 AR P050104647 A ARP050104647 A AR P050104647A AR P050104647 A ARP050104647 A AR P050104647A AR 051478 A1 AR051478 A1 AR 051478A1
Authority
AR
Argentina
Prior art keywords
explants
transformation
urophylla
selection
grandis
Prior art date
Application number
ARP050104647A
Other languages
Spanish (es)
Original Assignee
Arborgen Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/981,742 external-priority patent/US20060101536A1/en
Application filed by Arborgen Llc filed Critical Arborgen Llc
Publication of AR051478A1 publication Critical patent/AR051478A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente se relaciona con un método para transformar y seleccionar explantes. El método de transformacion incluye precultivar los explantes en la presencia de un inductor de Agrobacterium y exponer los explantes transformados a un medio de regeneracion de brotes que acelera el desarrollo de los mismos. Se proveen plantas regeneradas por este método de transformacion. En particular, métodos para obtener células transgénicas de E. grandis x E. urophylla y a partir de las mismas regenerar árboles de E. grandis x E. urophylla transformados de forma estable. La presente también provee medios, métodos y plásmidos para seleccionar y regenerar plantas.This is related to a method to transform and select explants. The transformation method includes precultivating the explants in the presence of an Agrobacterium inducer and exposing the transformed explants to a bud regeneration medium that accelerates their development. Regenerated plants are provided by this transformation method. In particular, methods to obtain transgenic cells of E. grandis x E. urophylla and from them regenerate trees of E. grandis x E. urophylla stably transformed. This also provides means, methods and plasmids for selecting and regenerating plants.

ARP050104647A 2004-11-05 2005-11-04 TRANSFORMATION AND SELECTION OF EUCALYPTUS UROPHYLLA AR051478A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/981,742 US20060101536A1 (en) 2004-11-05 2004-11-05 Eucalyptus urophylla transformation and regeneration
US11/158,342 US20060101537A1 (en) 2004-11-05 2005-06-22 Eucalyptus urophylla transformation and selection

Publications (1)

Publication Number Publication Date
AR051478A1 true AR051478A1 (en) 2007-01-17

Family

ID=36336974

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050104647A AR051478A1 (en) 2004-11-05 2005-11-04 TRANSFORMATION AND SELECTION OF EUCALYPTUS UROPHYLLA

Country Status (4)

Country Link
EP (1) EP1814379A4 (en)
AR (1) AR051478A1 (en)
AU (1) AU2005305071A1 (en)
WO (1) WO2006052554A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010129737A2 (en) * 2009-05-07 2010-11-11 Arborgen, Llc Materials and methods for regeneration and transformation of trees
CN103155872B (en) * 2013-04-12 2014-01-08 湛江师范学院 Eucalyptus urophylla*grandis embryoid induction seedling raising method
CN113430222A (en) * 2021-07-27 2021-09-24 中国林业科学研究院热带林业研究所 Eucalyptus transgenic method based on agrobacterium rhizogenes mediation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR9913740A (en) * 1998-09-15 2001-06-05 Genesis Res & Dev Corp Ltd Method for the production of genetically modified plants, plant materials and produced from them
JP4099898B2 (en) * 1999-05-07 2008-06-11 王子製紙株式会社 Methods for transforming adult Eucalyptus plants
CA2528408A1 (en) * 2003-06-06 2004-12-16 Arborgen, Llc Plant transformation and selection

Also Published As

Publication number Publication date
AU2005305071A1 (en) 2006-05-18
WO2006052554A2 (en) 2006-05-18
EP1814379A4 (en) 2009-02-25
EP1814379A2 (en) 2007-08-08
WO2006052554A3 (en) 2008-09-04

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