AR016185A1 - METHOD FOR PRODUCING PLANTS WITH MALE STERILITY; SUCH PLANTS; VEGETABLE CELLS THAT CONTAIN IN THEIR GENOMA A FIRST DNA MOLECULA, METODOPARA TO PRODUCE HYBRID SEEDS, SUCH SEEDS AND THE PLANTS ACHIEVED OF THESE, AND TRANSGENIC PLANTS AND SEEDS WITH THE CURRENT VEGETABLE CELLS - Google Patents
METHOD FOR PRODUCING PLANTS WITH MALE STERILITY; SUCH PLANTS; VEGETABLE CELLS THAT CONTAIN IN THEIR GENOMA A FIRST DNA MOLECULA, METODOPARA TO PRODUCE HYBRID SEEDS, SUCH SEEDS AND THE PLANTS ACHIEVED OF THESE, AND TRANSGENIC PLANTS AND SEEDS WITH THE CURRENT VEGETABLE CELLSInfo
- Publication number
- AR016185A1 AR016185A1 ARP990101003A AR016185A1 AR 016185 A1 AR016185 A1 AR 016185A1 AR P990101003 A ARP990101003 A AR P990101003A AR 016185 A1 AR016185 A1 AR 016185A1
- Authority
- AR
- Argentina
- Prior art keywords
- plants
- seeds
- rna sequence
- plant cells
- dna
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
- C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Virology (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Comprende un método para producir plantas con esterilidad masculina, caracterizada porque comprende los pasos de: insertar en el genoma de una célulavegetal: una primera molécula de ADN que comprende, ligado operativamente en la orientacion 5 a 3; unprimer promotor que funciona en células vegetalescausando la produccion de una primera secuencia de ARN; una primera secuencia de ADN que codifica una proteína que produce tolerancia al glifosato; una primeraregion no traducida 3 que funciona en células vegetales causando la polialdenilacion del extremo 3 de la primera secuencia de ARN; y una segunda molécula deADN que comprende, ligado operativamente en la orientacion 5 a 3; un segundo promotor que funciona en células vegetales causando la produccion de una segundasecuencia de ARN en un tejido reproductor masculino; una segunda secuencia de ADN que codifica una segunda secuencia de ARN que es complementaria de la primerasecuencia de ARN; una segunda region no traducida 3 que funciona encélulas vegetales causando la poliadenilacion del extremo 3 de la segunda secuencia deARN; obtener células vegetales transformadas que contienen la primera y segunda molécula de ADN; regenerrar plantas transformadas a partir de las célulasvegetales transformadas; y exponer las plantas transformadas a glifosato. Las plantas que contienen la primera y la segunda molécula de ADN se vuelvenmasculinos estériles por exposicion a glifosato. Los métodos y las composiciones de la invencion resultan ventajosos para su uso en la generacion de semillashíbridas, para restringir el cruzamiento con especies relacionadas y para prolongar la vida de las flores.It comprises a method for producing plants with male sterility, characterized in that it comprises the steps of: inserting into the genome of a vegetative cell: a first DNA molecule that comprises, operatively linked in orientation 5 to 3; a first promoter that works in plant cells causing the production of a first RNA sequence; a first DNA sequence that encodes a protein that produces glyphosate tolerance; a first untranslated region 3 that functions in plant cells causing polyaldenylation of end 3 of the first RNA sequence; and a second DNA molecule comprising, operably linked in the 5 to 3 orientation; a second promoter that works in plant cells causing the production of a second RNA sequence in a male reproductive tissue; a second DNA sequence encoding a second RNA sequence that is complementary to the first RNA sequence; a second untranslated region 3 that operates plant cells causing polyadenylation of end 3 of the second RNA sequence; obtain transformed plant cells that contain the first and second DNA molecules; regenerate transformed plants from transformed vegetable cells; and expose the transformed plants to glyphosate. Plants that contain the first and second DNA molecules become sterile by exposure to glyphosate. The methods and compositions of the invention are advantageous for use in the generation of hybrid seeds, to restrict crossbreeding with related species and to prolong the life of the flowers.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US7727798P | 1998-03-09 | 1998-03-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR016185A1 true AR016185A1 (en) | 2001-06-20 |
Family
ID=22137136
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP990101003 AR016185A1 (en) | 1998-03-09 | 1999-03-09 | METHOD FOR PRODUCING PLANTS WITH MALE STERILITY; SUCH PLANTS; VEGETABLE CELLS THAT CONTAIN IN THEIR GENOMA A FIRST DNA MOLECULA, METODOPARA TO PRODUCE HYBRID SEEDS, SUCH SEEDS AND THE PLANTS ACHIEVED OF THESE, AND TRANSGENIC PLANTS AND SEEDS WITH THE CURRENT VEGETABLE CELLS |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1062354A2 (en) |
CN (1) | CN1300324A (en) |
AR (1) | AR016185A1 (en) |
AU (1) | AU2992999A (en) |
BR (1) | BR9908631A (en) |
WO (1) | WO1999046396A2 (en) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR034125A1 (en) | 2000-07-13 | 2004-02-04 | Pioneer Hi Bred Int | ISOLATED POLINUCLEOTIDE OF ZEA MAYS (ZMAXIG1) AND ITS APPLICATION IN THE GENETIC REGULATION OF PLANTS. |
EP1174512A1 (en) * | 2000-07-17 | 2002-01-23 | Peter Stamp | Seed composition and method for reducing or preventing the release of genetically manipulated pollen |
US6646186B1 (en) | 2000-07-26 | 2003-11-11 | Stine Seed Farm Inc. | Hybrid soybeans and methods of production |
US6689880B2 (en) | 2000-09-29 | 2004-02-10 | Monsanto Technology Llc | DNA molecule for detecting glyphosate tolerant wheat plant 33391 and progeny thereof |
WO2002045510A2 (en) * | 2000-12-07 | 2002-06-13 | D & Pl Technology Holding Corporation | Glyphosate-induced male sterility in dicots |
US7230168B2 (en) | 2001-12-20 | 2007-06-12 | The Curators Of The University Of Missouri | Reversible male sterility in transgenic plants by expression of cytokinin oxidase |
DE10163163A1 (en) | 2001-12-20 | 2003-07-03 | Basf Ag | Process for the production of highly functional, hyperbranched polyester by enzymatic esterification |
SI1481068T1 (en) | 2002-02-26 | 2011-05-31 | Syngenta Ltd | A method of selectively producing male or female sterile plants |
MXPA05008519A (en) * | 2003-02-12 | 2005-10-20 | Monsanto Technology Llc | Cotton event mon 88913 and compositions and methods for detection thereof. |
WO2004082366A2 (en) * | 2003-03-19 | 2004-09-30 | Regents Of The University Of Minnesota | Methods to confer enhanced floral properties to plants |
US20060200878A1 (en) | 2004-12-21 | 2006-09-07 | Linda Lutfiyya | Recombinant DNA constructs and methods for controlling gene expression |
ZA200803234B (en) * | 2005-10-13 | 2009-01-28 | Monsanto Technology Llc | Methods for producing hybrid seed |
US8334430B2 (en) | 2005-10-13 | 2012-12-18 | Monsanto Technology Llc | Methods for producing hybrid seed |
US20100257621A1 (en) * | 2006-10-03 | 2010-10-07 | Monsanto Technology Llc | Methods for Hybrid Corn Seed Production and Compositions Produced Therefrom |
US8946511B2 (en) | 2006-10-12 | 2015-02-03 | Monsanto Technology Llc | Plant microRNAs and methods of use thereof |
MX2012005906A (en) | 2009-11-23 | 2012-06-19 | Monsanto Technology Llc | Transgenic maize event mon 87427 and the relative development scale. |
AR088133A1 (en) * | 2011-07-01 | 2014-05-14 | Monsanto Technology Llc | METHODS AND COMPOSITIONS FOR THE SELECTIVE REGULATION OF PROTEIN EXPRESSION |
CN103222423B (en) * | 2013-05-14 | 2014-11-12 | 安徽省农业科学院水稻研究所 | Rice hybrid breeding material as well as cultivation method and application thereof |
CN114891758B (en) | 2015-07-22 | 2024-02-13 | 孟山都技术公司 | Methods and compositions for selectively modulating protein expression |
CN112080501B (en) * | 2020-09-10 | 2021-11-23 | 隆平生物技术(海南)有限公司 | Recombinant promoter, gene expression cassette and application of recombinant promoter and gene expression cassette in plant breeding |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ227835A (en) * | 1988-02-03 | 1992-09-25 | Paladin Hybrids Inc | Antisense gene systems of pollination control for hybrid seed production |
RU2146290C1 (en) * | 1990-08-31 | 2000-03-10 | Монсанто Компани | Isolated dna sequence (variants), recombinant double-stranded dna molecule and method of preparing genetically transformed plants |
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1999
- 1999-03-09 EP EP99911240A patent/EP1062354A2/en not_active Withdrawn
- 1999-03-09 BR BR9908631-0A patent/BR9908631A/en not_active IP Right Cessation
- 1999-03-09 WO PCT/US1999/005126 patent/WO1999046396A2/en not_active Application Discontinuation
- 1999-03-09 AR ARP990101003 patent/AR016185A1/en unknown
- 1999-03-09 AU AU29929/99A patent/AU2992999A/en not_active Abandoned
- 1999-03-09 CN CN 99806006 patent/CN1300324A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
EP1062354A2 (en) | 2000-12-27 |
WO1999046396A3 (en) | 1999-10-21 |
WO1999046396A2 (en) | 1999-09-16 |
BR9908631A (en) | 2000-11-14 |
CN1300324A (en) | 2001-06-20 |
AU2992999A (en) | 1999-09-27 |
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