WO2015065194A1 - Fermented formula with non digestible oligosaccharides - Google Patents
Fermented formula with non digestible oligosaccharides Download PDFInfo
- Publication number
- WO2015065194A1 WO2015065194A1 PCT/NL2014/050762 NL2014050762W WO2015065194A1 WO 2015065194 A1 WO2015065194 A1 WO 2015065194A1 NL 2014050762 W NL2014050762 W NL 2014050762W WO 2015065194 A1 WO2015065194 A1 WO 2015065194A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- formula
- infant
- follow
- oligosaccharides
- lactic acid
- Prior art date
Links
- 229920001542 oligosaccharide Polymers 0.000 title claims abstract description 110
- 150000002482 oligosaccharides Chemical class 0.000 title claims abstract description 78
- 235000013350 formula milk Nutrition 0.000 claims abstract description 196
- 235000020218 follow-on milk formula Nutrition 0.000 claims abstract description 146
- 206010011469 Crying Diseases 0.000 claims abstract description 61
- 208000002881 Colic Diseases 0.000 claims abstract description 47
- 208000004998 Abdominal Pain Diseases 0.000 claims abstract description 43
- 230000003247 decreasing effect Effects 0.000 claims abstract description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 154
- 239000004310 lactic acid Substances 0.000 claims description 76
- 235000014655 lactic acid Nutrition 0.000 claims description 76
- 239000000203 mixture Substances 0.000 claims description 68
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 claims description 53
- 241000894006 Bacteria Species 0.000 claims description 42
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims description 40
- 239000004615 ingredient Substances 0.000 claims description 39
- 235000016709 nutrition Nutrition 0.000 claims description 19
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 18
- 229940116871 l-lactate Drugs 0.000 claims description 17
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical class OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 16
- 235000021255 galacto-oligosaccharides Nutrition 0.000 claims description 13
- 150000003271 galactooligosaccharides Chemical class 0.000 claims description 12
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 102000005936 beta-Galactosidase Human genes 0.000 claims description 7
- 230000035764 nutrition Effects 0.000 claims description 7
- 241000186012 Bifidobacterium breve Species 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 229920001353 Dextrin Polymers 0.000 claims description 2
- 239000004375 Dextrin Substances 0.000 claims description 2
- 235000019425 dextrin Nutrition 0.000 claims description 2
- 150000003272 mannan oligosaccharides Chemical class 0.000 claims description 2
- 235000005911 diet Nutrition 0.000 description 39
- 230000037213 diet Effects 0.000 description 39
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 25
- 235000018102 proteins Nutrition 0.000 description 25
- 102000004169 proteins and genes Human genes 0.000 description 25
- 108090000623 proteins and genes Proteins 0.000 description 25
- 239000008101 lactose Substances 0.000 description 24
- 150000001720 carbohydrates Chemical class 0.000 description 21
- 235000014633 carbohydrates Nutrition 0.000 description 21
- 239000007788 liquid Substances 0.000 description 15
- 108010046377 Whey Proteins Proteins 0.000 description 14
- 239000005018 casein Substances 0.000 description 14
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 14
- 235000021240 caseins Nutrition 0.000 description 14
- 235000021119 whey protein Nutrition 0.000 description 12
- 102000007544 Whey Proteins Human genes 0.000 description 11
- 238000000855 fermentation Methods 0.000 description 11
- 230000004151 fermentation Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 9
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 description 9
- 229940107187 fructooligosaccharide Drugs 0.000 description 9
- 150000002632 lipids Chemical class 0.000 description 9
- 235000015140 cultured milk Nutrition 0.000 description 8
- 235000013336 milk Nutrition 0.000 description 8
- 239000008267 milk Substances 0.000 description 8
- 210000004080 milk Anatomy 0.000 description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 241000283690 Bos taurus Species 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 229920001202 Inulin Polymers 0.000 description 6
- 241000186660 Lactobacillus Species 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 6
- 229940029339 inulin Drugs 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000000546 chi-square test Methods 0.000 description 5
- 230000035611 feeding Effects 0.000 description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 description 5
- 206010021746 Infantile colic Diseases 0.000 description 4
- 229920002774 Maltodextrin Polymers 0.000 description 4
- 208000009793 Milk Hypersensitivity Diseases 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- -1 hexose sugars Chemical class 0.000 description 4
- 235000020256 human milk Nutrition 0.000 description 4
- 210000004251 human milk Anatomy 0.000 description 4
- 150000007524 organic acids Chemical class 0.000 description 4
- 235000005985 organic acids Nutrition 0.000 description 4
- 235000004252 protein component Nutrition 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 3
- OIZGSVFYNBZVIK-FHHHURIISA-N 3'-sialyllactose Chemical compound O1[C@@H]([C@H](O)[C@H](O)CO)[C@H](NC(=O)C)[C@@H](O)C[C@@]1(C(O)=O)O[C@@H]1[C@@H](O)[C@H](O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O[C@H](CO)[C@@H]1O OIZGSVFYNBZVIK-FHHHURIISA-N 0.000 description 3
- 102100026189 Beta-galactosidase Human genes 0.000 description 3
- 241000186000 Bifidobacterium Species 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- 108010059881 Lactase Proteins 0.000 description 3
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 3
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 3
- 239000005913 Maltodextrin Substances 0.000 description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 3
- 238000000585 Mann–Whitney U test Methods 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 3
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 3
- 235000020940 control diet Nutrition 0.000 description 3
- 235000020247 cow milk Nutrition 0.000 description 3
- 239000003925 fat Substances 0.000 description 3
- 235000019197 fats Nutrition 0.000 description 3
- 229930182830 galactose Natural products 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 229940116108 lactase Drugs 0.000 description 3
- 235000020778 linoleic acid Nutrition 0.000 description 3
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 3
- 235000020978 long-chain polyunsaturated fatty acids Nutrition 0.000 description 3
- 229940035034 maltodextrin Drugs 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 235000019871 vegetable fat Nutrition 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- SNFSYLYCDAVZGP-OLAZETNGSA-N 2'-fucosyllactose Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)O[C@H](CO)[C@H](O)[C@@H]1O SNFSYLYCDAVZGP-OLAZETNGSA-N 0.000 description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 2
- 241000416162 Astragalus gummifer Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 101710088194 Dehydrogenase Proteins 0.000 description 2
- 238000000729 Fisher's exact test Methods 0.000 description 2
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 2
- 229920002907 Guar gum Polymers 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 2
- 244000199866 Lactobacillus casei Species 0.000 description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 description 2
- 240000002605 Lactobacillus helveticus Species 0.000 description 2
- 235000013967 Lactobacillus helveticus Nutrition 0.000 description 2
- 241000186605 Lactobacillus paracasei Species 0.000 description 2
- 240000006024 Lactobacillus plantarum Species 0.000 description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 2
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 2
- 241000186612 Lactobacillus sakei Species 0.000 description 2
- 241000186869 Lactobacillus salivarius Species 0.000 description 2
- 241000194036 Lactococcus Species 0.000 description 2
- 229920000161 Locust bean gum Polymers 0.000 description 2
- 201000010859 Milk allergy Diseases 0.000 description 2
- 235000019484 Rapeseed oil Nutrition 0.000 description 2
- 244000057717 Streptococcus lactis Species 0.000 description 2
- 235000014897 Streptococcus lactis Nutrition 0.000 description 2
- 229920001615 Tragacanth Polymers 0.000 description 2
- 244000250129 Trigonella foenum graecum Species 0.000 description 2
- 235000001484 Trigonella foenum graecum Nutrition 0.000 description 2
- SNFSYLYCDAVZGP-UHFFFAOYSA-N UNPD26986 Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(OC(O)C(O)C2O)CO)OC(CO)C(O)C1O SNFSYLYCDAVZGP-UHFFFAOYSA-N 0.000 description 2
- 239000005862 Whey Substances 0.000 description 2
- 208000026935 allergic disease Diseases 0.000 description 2
- 230000007815 allergy Effects 0.000 description 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 2
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000021120 animal protein Nutrition 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- CURUTKGFNZGFSE-UHFFFAOYSA-N dicyclomine Chemical compound C1CCCCC1C1(C(=O)OCCN(CC)CC)CCCCC1 CURUTKGFNZGFSE-UHFFFAOYSA-N 0.000 description 2
- 229960002777 dicycloverine Drugs 0.000 description 2
- FCIROHDMPFOSFG-LAVSNGQLSA-N disialyllacto-N-tetraose Chemical compound O1[C@@H]([C@H](O)[C@H](O)CO)[C@H](NC(=O)C)[C@@H](O)C[C@@]1(C(O)=O)OC[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@]3(O[C@H]([C@H](NC(C)=O)[C@@H](O)C3)[C@H](O)[C@H](O)CO)C(O)=O)[C@@H](O)[C@@H](CO)O2)O)[C@@H](NC(C)=O)[C@H](O[C@@H]2[C@H]([C@H](O[C@H]3[C@@H]([C@@H](O)C(O)O[C@@H]3CO)O)O[C@H](CO)[C@@H]2O)O)O1 FCIROHDMPFOSFG-LAVSNGQLSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- BJHIKXHVCXFQLS-UYFOZJQFSA-N fructose group Chemical group OCC(=O)[C@@H](O)[C@H](O)[C@H](O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 description 2
- 230000002641 glycemic effect Effects 0.000 description 2
- 235000010417 guar gum Nutrition 0.000 description 2
- 239000000665 guar gum Substances 0.000 description 2
- 229960002154 guar gum Drugs 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 235000021244 human milk protein Nutrition 0.000 description 2
- 229930187173 lacto-N-Difucosylhexaose Natural products 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 2
- 229940017800 lactobacillus casei Drugs 0.000 description 2
- 229940054346 lactobacillus helveticus Drugs 0.000 description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 description 2
- 235000010420 locust bean gum Nutrition 0.000 description 2
- 239000000711 locust bean gum Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000011785 micronutrient Substances 0.000 description 2
- 235000013369 micronutrients Nutrition 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid group Chemical group C(CCCCCCC\C=C/CCCCCCCC)(=O)O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
- 229920000157 polyfructose Polymers 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 235000010491 tara gum Nutrition 0.000 description 2
- 239000000213 tara gum Substances 0.000 description 2
- 235000021195 test diet Nutrition 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 235000001019 trigonella foenum-graecum Nutrition 0.000 description 2
- 235000013618 yogurt Nutrition 0.000 description 2
- JVTAAEKCZFNVCJ-UWTATZPHSA-M (R)-lactate Chemical compound C[C@@H](O)C([O-])=O JVTAAEKCZFNVCJ-UWTATZPHSA-M 0.000 description 1
- JVTAAEKCZFNVCJ-REOHCLBHSA-M (S)-lactate Chemical compound C[C@H](O)C([O-])=O JVTAAEKCZFNVCJ-REOHCLBHSA-M 0.000 description 1
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical group OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 description 1
- 229940062827 2'-fucosyllactose Drugs 0.000 description 1
- HWHQUWQCBPAQQH-UHFFFAOYSA-N 2-O-alpha-L-Fucosyl-lactose Natural products OC1C(O)C(O)C(C)OC1OC1C(O)C(O)C(CO)OC1OC(C(O)CO)C(O)C(O)C=O HWHQUWQCBPAQQH-UHFFFAOYSA-N 0.000 description 1
- IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 description 1
- KUWPCJHYPSUOFW-YBXAARCKSA-N 2-nitrophenyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1[N+]([O-])=O KUWPCJHYPSUOFW-YBXAARCKSA-N 0.000 description 1
- AUNPEJDACLEKSC-ZAYDSPBTSA-N 3-fucosyllactose Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)O[C@H](CO)[C@@H]1O AUNPEJDACLEKSC-ZAYDSPBTSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- DPUOLQHDNGRHBS-UHFFFAOYSA-N Brassidinsaeure Natural products CCCCCCCCC=CCCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-UHFFFAOYSA-N 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 108010016626 Dipeptides Proteins 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- URXZXNYJPAJJOQ-UHFFFAOYSA-N Erucic acid Natural products CCCCCCC=CCCCCCCCCCCCC(O)=O URXZXNYJPAJJOQ-UHFFFAOYSA-N 0.000 description 1
- 229920002670 Fructan Polymers 0.000 description 1
- 229920000855 Fucoidan Polymers 0.000 description 1
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 1
- 238000007696 Kjeldahl method Methods 0.000 description 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 240000001929 Lactobacillus brevis Species 0.000 description 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 1
- LKOHREGGXUJGKC-UHFFFAOYSA-N Lactodifucotetraose Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(C(O)C(O)OC2CO)OC2C(C(O)C(O)C(C)O2)O)OC(CO)C(O)C1O LKOHREGGXUJGKC-UHFFFAOYSA-N 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 102000014171 Milk Proteins Human genes 0.000 description 1
- 108010011756 Milk Proteins Proteins 0.000 description 1
- 208000007101 Muscle Cramp Diseases 0.000 description 1
- 241000202223 Oenococcus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
- 201000009916 Postpartum depression Diseases 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 235000019904 Raftiline® Nutrition 0.000 description 1
- 235000019485 Safflower oil Nutrition 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 235000019486 Sunflower oil Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- LKOHREGGXUJGKC-GXSKDVPZSA-N alpha-L-Fucp-(1->3)-[alpha-L-Fucp-(1->2)-beta-D-Galp-(1->4)]-beta-D-Glcp Chemical compound C[C@@H]1O[C@@H](O[C@@H]2[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]2O[C@@H]2[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]2O[C@@H]2O[C@@H](C)[C@@H](O)[C@@H](O)[C@@H]2O)[C@@H](O)[C@H](O)[C@@H]1O LKOHREGGXUJGKC-GXSKDVPZSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000001078 anti-cholinergic effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 235000019577 caloric intake Nutrition 0.000 description 1
- 239000000828 canola oil Substances 0.000 description 1
- 235000019519 canola oil Nutrition 0.000 description 1
- 230000004098 cellular respiration Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- AJNVQOSZGJRYEI-UHFFFAOYSA-N digallium;oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[Ga+3].[Ga+3] AJNVQOSZGJRYEI-UHFFFAOYSA-N 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 230000027721 electron transport chain Effects 0.000 description 1
- DPUOLQHDNGRHBS-KTKRTIGZSA-N erucic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-KTKRTIGZSA-N 0.000 description 1
- 235000004626 essential fatty acids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 229940097043 glucuronic acid Drugs 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000010243 gut motility Effects 0.000 description 1
- 235000020215 hypoallergenic milk formula Nutrition 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 235000021125 infant nutrition Nutrition 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 1
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- 238000007477 logistic regression Methods 0.000 description 1
- 235000021073 macronutrients Nutrition 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000021243 milk fat Nutrition 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 235000019865 palm kernel oil Nutrition 0.000 description 1
- 239000003346 palm kernel oil Substances 0.000 description 1
- 235000020615 partially hydrolysed formula Nutrition 0.000 description 1
- 150000003904 phospholipids Chemical group 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000003813 safflower oil Substances 0.000 description 1
- 235000005713 safflower oil Nutrition 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 229940001941 soy protein Drugs 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000002600 sunflower oil Substances 0.000 description 1
- 125000005457 triglyceride group Chemical group 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 235000020138 yakult Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/40—Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- the present invention is in the field of infant nutrition.
- Infantile colic is a widespread clinical problem in infants. It is the cause of 10 to 20 % of all early paediatrician visits and often leads to parental exhaustion and stress. An increased risk of maternal postpartum depression has been observed. Infantile colic is observed in 10 to 30 % of the infants, who are otherwise healthy. Typically colic in an infant occurs between the age of 2 weeks to 4 months. The causes of infantile colics are not fully understood, and this makes it difficult to find a good prevention or treatment. Anticholinergic drugs such as dicyclomine and dicycloverine are sometimes used, but they are not recommended because of their serious side effects.
- cow's milk allergy is the underlying cause of the colics, a hypoallergenic formula may solve the problem.
- cow's milk allergy is only present in a small population of the infants suffering from colics.
- EP 2 520 181 discloses the use of a fermented or partly fermented infant formula for promoting gut health, including amongst others the treatment and/or prevention of colics or cramps.
- Savino et al, 2006, Eur J Clin Nutr 60, 1304-1310 disclose that the use of a partially hydrolysed formula supplemented with fructo- and galacto-oligosaccharides and comprising low lactose and increased palmitic acid at the beta position induces a reduction in colic episodes, measured as crying episodes, in infants with colics after 7 and 14 days.
- EP 1685763 disclosed the use of a combination of GOS and polyfructose for amongst others colic and/or abdominal cramps.
- WO 2010/008278 discloses the use of antibiotics or probiotics for the treatment of gut motility disorders.
- the present inventors found that when a partly fermented infant formula comprising at least 2 non digestible oligosaccharides was administered to infants, the incidence of colics and the number of crying episodes per day was statistically significantly reduced compared to controls that contained either only a partly fermented infant formula without non-digestible oligosaccharides, or a non-fermented infant formula that contained non-digestible oligosaccharides.
- the reduction was unexpectedly much higher than observed when partly fermented infant formula without non digestible oligosaccharides were given, or when non- fermented infant formula with non digestible oligosaccharides were given.
- the present invention concerns a method for reducing the incidence of colics in an infant, comprising administering to said infant an infant formula or follow on formula comprising:
- a fermented ingredient in particular a composition fermented by lactic acid bacteria, - 0.5 to 20 wt.% non digestible oligosaccharides based on dry weight of the infant formula or follow on formula, and
- the present invention concerns the use of a fermented ingredient, in particular a composition fermented by lactic acid bacteria, and non-digestible oligosaccharides in the manufacture of an infant or follow on formula for use in reducing the incidence of colics in an infant, wherein the infant formula or follow on formula comprises:
- the invention can also be worded as an infant formula or follow on formula comprising:
- a fermented ingredient in particular a composition fermented by lactic acid bacteria
- the infant formula or follow on formula is for providing nutrition to an infant with an age of 6 months or below.
- the infant is suffering from colics or is at risk of developing colics.
- the infant is suffering from colics.
- the present invention also concerns a method for reducing the number of crying episodes per day in an infant, comprising administering to said infant an infant formula or follow on formula comprising:
- a fermented ingredient in particular a composition fermented by lactic acid bacteria
- the invention also concerns the use of a fermented ingredient, in particular a composition fermented by lactic acid bacteria, and non-digestible oligosaccharides in the manufacture of an infant or follow on formula for use in reducing the number of crying episodes per day in the infant, wherein the infant formula or follow on formula comprises: - 0.5 to 20 wt.% non digestible oligosaccharides based on dry weight of the infant formula or follow on formula, and
- the invention can also be worded as an infant formula or follow on formula comprising:
- a fermented ingredient in particular a composition fermented by lactic acid bacteria
- the use for reducing the number of crying episodes per day is for reducing the incidence of colics in the infant.
- reducing the number of crying episodes per day can also be referred to as reducing crying.
- the present invention also concerns a method for decreasing the duration of crying in an infant, comprising administering to said infant an infant formula or follow on formula comprising:
- a fermented ingredient in particular a composition fermented by lactic acid bacteria
- the invention also concerns the use of a fermented ingredient, in particular a composition fermented by lactic acid bacteria, and non-digestible oligosaccharides in the manufacture of an infant or follow on formula for use in decreasing the duration of crying in the infant, wherein the infant formula or follow on formula comprises:
- the invention can also be worded as an infant formula or follow on formula comprising:
- a fermented ingredient in particular a composition fermented by lactic acid bacteria
- the use for decreasing the duration of crying is for reducing the incidence of colics in the infant.
- the infant formula or follow on formula is for providing nutrition to an infant with an age of 6 months or below.
- the infant is suffering from crying episodes or is at risk of developing crying episodes.
- the infant is suffering from crying episodes.
- Fermentation is the process of deriving energy from the oxidation of carbohydrates, such as the lactose present in milk, using an endogenous electron acceptor, which is usually an organic compound. This is in contrast to cellular respiration, where electrons are donated to an exogenous electron acceptor, such as oxygen, via an electron transport chain.
- fermentation of a composition by lactic acid producing bacteria has the common meaning of the conversion of carbohydrates present in the composition to organic acids.
- organic acids formed may comprise, besides lactic acid, also other organic acids such as acetic acid.
- organic acids are understood to include the corresponding conjugate bases (e.g. lactic acid may include lactate and acetic acid may include acetate).
- a fermented ingredient is used.
- the fermented ingredient is a composition that is fermented by lactic acid bacteria.
- the composition that is fermented by lactic acid bacteria comprises lactose and/or protein, preferably the composition that is fermented by lactic acid bacteria comprises at least lactose.
- the composition that is fermented by lactic acid bacteria comprises lactose and protein.
- the composition that is fermented by lactic acid bacteria is a milk-derived composition.
- the carbohydrate that is fermented is preferably lactose.
- 'fermented' is mentioned, 'fermented by lactic acid bacteria' is meant.
- Lactic acid bacteria are also referred to as lactic acid producing bacteria and include bacteria of the genus Streptococcus, Lactococcus, Lactobacillus, Leuconostoc, Enterococcus, Oenococcus, Pediococcus, and Bifidobacterium.
- the infant or follow on formula comprises a fermented ingredient.
- the infant or follow on formula in the method or use according to the present invention preferably comprises a fermented milk-derived composition.
- This fermented milk- derived composition is obtained by incubation of a combination of milk, e.g. skim milk, or a milk-derived product, e.g.
- lactic acid bacterium such as lactococci, lactobacilli, streptococci and bifidobacteria
- the fermented milk-derived composition is obtained by incubation with at least one strain selected from lactococci, lactobacilli and streptococci, preferably with at least one strain selected from streptococci.
- lactic acid bacteria which perform homolactic fermentation are used for fermentation, since in this case two lactic acid molecules are produced per sugar moiety and no gas is formed.
- Homolactic lactic acid bacteria include Streptococcus thermophilus , Lactococcus species, preferably Lactococcus lactis, and Group I lactobacilli such as Lactobacillus acidophilus, Lactobacillus helveticus and Lactobacillus salivarius and facultative heterofermentative Lactobacilli which produce two lactic acid from hexose sugars, and which include Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus sakei.
- the fermented milk-derived composition is obtained by incubation of a combination of milk, e.g.
- skim milk or a milk derived product, e.g. whey, with at least one strain selected from Streptococcus thermophilus, Lactococcus lactis, Lactobacillus acidophilus, Lactobacillus helveticus, Lactobacillus salivarius, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus plantarum and Lactobacillus sakei, preferably the fermented milk-derived composition is obtained by incubation with Streptococcus thermophilus. Preferably the combination is incubated for 10 minutes to about 6 hours. The temperature during incubation is preferably between 20 and 50 °C.
- the incubated composition is preferably subjected to a heat treatment.
- a heat treatment preferably at least 90 % of living lactic acid bacteria are inactivated, more preferably at least 95 %.
- the fermented ingredient, more preferably the infant formula or follow on formula comprises less than lxlO 5 colony forming units (cfu) lactic acid bacteria per g dry weight.
- the heat treatment preferably is performed at a temperature between 80 and 180 °C. Inactivation of the lactic acid bacterium advantageously results in less post acidification and a safer product. This is especially advantageous since the infant or follow on formula that is manufactured is to be administered to infants.
- the fermented ingredient comprises inactivated lactic acid bacteria.
- Procedures to prepare fermented ingredients suitable for the purpose of the present invention are known per se.
- EP 778885 which is incorporated herein by reference, discloses in particular in example 7 a suitable process for preparing a fermented ingredient.
- FR 2723960 which is incorporated herein by reference, discloses in particular in example 6 a suitable process for preparing a fermented ingredient.
- a milk derived composition preferably pasteurised, containing lactose and optionally further macronutrients such as fats, preferably vegetable fats, casein, whey protein, vitamins and/or minerals etc. is concentrated, e.g. to between 15 to 50% dry matter and then inoculated with S. thermophilus, for example with 5% of a culture containing 10 6 to 10 10 bacteria per ml.
- this milk derived composition comprises milk protein peptides.
- Temperature and duration of fermentation are as mentioned above.
- the fermented ingredient may be pasteurised or sterilized and for example spray dried or lyophilised to provide a form suitable to be formulated in the end product.
- thermophilus that are preferably used to prepare the fermented ingredient for the purpose of the present invention develop beta-galactosidase activity in the course of fermentation of the substrate.
- beta-galactosidase activity develops in parallel with acidity.
- Selection of a suitable strain of S. thermophilus is described in example 2 of EP 778885 and in example 1 of FR 2723960. It is preferred that in the infant or follow on formula according to the method or use of the invention, at least part of the beta-galactosidase activity that has developed during fermentation is retained.
- the at least part of the beta- galactosidase activity that is retained is lactase activity.
- lactase activity in the infant or follow on formula has a beneficial effect on promoting intestinal tract health.
- the infant or follow on formula comprises 0.2 - 4 U (units) beta-galactosidase activity
- the infant or follow on formula comprises per gram dry weight 0.2 - 4 U lactase activity.
- a beta-galactosidase activity of 1.0 U (one unit) corresponds to the capacity to hydrolyze 1.0 ⁇ of o-nitrophenyl ⁇ -D- galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
- the infant or follow on formula comprises at most lxlO 5 cfu living bacteria of S. thermophilus, preferably the infant or follow on formula comprises at most lxlO 4 cfu living bacteria of S. thermophilus based on g dry weight. In one embodiment, preferably not all lactic acid bacteria, preferably not all S. thermophilus, is inactivated. Thus preferably the infant or follow on formula comprises at least 1x10 cfu living bacteria of S. thermophilus, preferably the infant or follow on formula comprises at least 1x10 cfu living bacteria of S. thermophilus per g dry weight.
- Preferred strains of S. thermophilus to prepare a fermented ingredient, preferably a fermented milk-derived composition, for the purpose of the present invention have been deposited by Compagnie Gervais Danone at the Collection Nationale de Cultures de Microorganismes (CNCM) run by the Institut Pasteur, 25 rue du Dondel Roux, Paris, France on 23 August 1995 under the accession number 1-1620 and on 25 August 1994 under the accession number 1-1470.
- the strains 1-1620 and 1-1470 have been disclosed in EP 0778885.
- the composition additionally is supplemented with or fermented by one or more further strains of lactic acid bacteria.
- the one or more further strains are each a different strain than the first strain used for fermenting the composition as described above, preferably a different strain than S. thermophilics.
- Such different strains of lactic acid bacteria are preferably selected from the group consisting of Lactobacillus and Bifidobacteria, more preferably Bifidobacterium breve, most preferably Bifidobacterium breve strain deposited by Compagnie Gervais Danone at the CNCM under number 1-2219 on 31 May, 1999. Strain 1-2219 has been disclosed in EP 1615657.
- the fermented ingredient comprises Streptococcus thermophilus , and/or Bifidobacterium breve. In one embodiment, the fermented ingredient is fermented by Streptococcus thermophilus, and/or Bifidobacterium breve.
- the infant or follow on formula comprises a fermented ingredient, wherein the lactic acid bacteria are inactivated after fermentation.
- the composition that is fermented by lactic acid bacteria is not fermented by Lactobacillus bulgaricus.
- L. bulgaricus fermented products are considered not suitable for infants, since in young infants the specific dehydrogenase that converts D-lactate to pyruvate is far less active than the dehydrogenase which converts L-lactate.
- the fermented ingredient preferably comprises protein.
- the protein is preferably selected from the group consisting of non-human animal proteins, preferably non-human milk proteins.
- the fermented composition preferably contains casein, and/or whey protein, more preferably bovine whey proteins and/or bovine casein.
- the infant or follow on formula for use according to the present invention preferably comprises 25 to 100 wt.%, more preferably 30 to 99.5 wt.%, of the fermented ingredient, preferably the fermented milk-derived composition, based on dry weight of the infant or follow on formula.
- the present infant or follow on formula preferably contains 30 to 99 wt.%, more preferably 30 to 70 wt.%, more preferably 40 to 60 wt.%, of the fermented ingredient, based on dry weight of the final infant or follow on formula.
- Higher concentrations of fermented composition, e.g. 25, preferably 30 wt.% or above) advantageously improve the effect on colics incidence and crying episodes.
- the pH of the present infant or follow on formula is preferably between 5.0 and 7.5, more preferably between 5.0 and 7.0, even more preferably between 5.5 and 7.0, most preferably between 5.5 and 6.0.
- the infant or follow on formula for use according to the present invention comprises lactic acid and/or lactate. Lactic acid and/or lactate is formed upon fermentation by lactic acid bacteria.
- the present infant or follow on formula comprises 0.25 to 1.5 wt.% of the sum of lactic acid and lactate, preferably 0.30 to 1.0 wt.%, more preferably 0.4 to 0.7 wt.% of the sum of lactic acid and lactate based on dry weight of the infant or follow on formula.
- the more lactate and lactic acid is present the more the infant or follow on formula comprises of the fermented ingredient.
- Preferably at least 50 wt. %, even more preferably at least 90 wt.%, of the sum of lactic acid and lactate is in the form of L-isomer.
- the sum of L-lactic acid and L-lactate is more than 50 wt. %, more preferably more than 90 wt.%, based on the sum of total lactic acid and lactate.
- L-lactate and L-lactic acid is the same as L-(+)-lactate and L-(+) lactic acid.
- the infant or follow on formula comprises from 0.25 to 1.5 wt.% of the sum of lactate and lactic acid, preferably 0.30 to 1.0 wt.%, of the sum of lactate and lactic acid based on dry weight of the infant or follow on formula and wherein the sum of L-lactic acid and L-lactate is more than 50 wt. % based on the sum of total lactic acid and lactate.
- the infant or follow on formula comprises non-digestible oligosaccharides.
- Non-digestible oligosaccharides were found to synergistically act with the fermented ingredient to reduce the incidence of colics and number of crying episodes per day.
- the non-digestible oligosaccharides are water-soluble (according to the method disclosed in L. Prosky et al, J. Assoc. Anal. Chem 71: 1017-1023, 1988) and are preferably oligosaccharides with a degree of polymerisation (DP) of 2 to 200.
- DP degree of polymerisation
- the average DP of the non-digestible oligosaccharides are preferably below 200, more preferably below 100, even more preferably below 60, most preferably below 40.
- the non-digestible oligosaccharides are not digested in the intestine by the action of digestive enzymes present in the human upper digestive tract (small intestine and stomach).
- the non-digestible oligosaccharides are fermented by the human intestinal microbiota. For example, glucose, fructose, galactose, sucrose, lactose, maltose and the maltodextrins are considered digestible.
- the non-digestible oligosaccharide raw materials may comprise monosaccharides such as glucose, fructose, fucose, galactose, rhamnose, xylose, glucuronic acid, GalNac etc., but these are not part of the non digestible oligosaccharide fraction of the infant or follow on formula according to the present invention.
- the non-digestible oligosaccharides included in the infant or follow on formula according to the methods or use according to the present invention preferably include a mixture of at least two different non-digestible oligosaccharides.
- the non-digestible oligosaccharides are preferably selected from the group consisting of fructo- oligosaccharides (such as inulin), non-digestible dextrins, galacto-oligosaccharides (such as transgalacto-oligosaccharides), xylo-oligosaccharides, arabino-oligosaccharides, arabinogalacto- oligosaccharides, gluco-oligosaccharides, gentio-oligosaccharides, glucomanno- oligosaccharides, galactomanno-oligosaccharides, mannan-oligosaccharides, isomalto- oligosaccharides, nigero-oligosaccharides, chito-oligosaccharides, soy oligosaccharides, uronic acid oligosaccharides, sialyloligosaccharides (such as 3-sialyllactos
- the non digestible oligosaccharides consist of transgalacto-oligosaccharide, inulin and/or uronic acid oligosaccharides, most preferably at least transgalacto-oligosaccharides are included. In one embodiment, the non digestible oligosaccharides are selected from the group consisting of transgalacto-oligosaccharides, fructo-oligosaccharides and galacturonic acid oligosaccharides and mixtures of thereof.
- the non-digestible oligosaccharides are preferably selected from the group consisting of ⁇ - galacto-oligosaccharide, a-galacto-oligosaccharide, and galactan. According to a more preferred embodiment non-digestible oligosaccharides are ⁇ -galacto-oligosaccharide.
- the non- digestible oligosaccharides comprises galacto-oligosaccharides with ⁇ (1,4), ⁇ ( 1 ,3) and/or ⁇ (1,6) glycosidic bonds and a terminal glucose.
- Transgalacto-oligosaccharide is for example available under the trade name Vivinal®GOS (Borculo Domo Ingredients, Zwolle, Netherlands), Bi2muno (Clasado), Cup-oligo (Nissin Sugar) and 01igomate55 (Yakult).
- the non-digestible oligosaccharides preferably comprise fructo-oligosaccharides.
- a fructo- oligosaccharide may in other context have names like fructopolysaccharides, oligofructose, polyfructose, polyfructan, inulin, levan and fructan and may refer to oligosaccharides comprising ⁇ -linked fructose units, which are preferably linked by ⁇ (2, 1) and/or ⁇ (2,6) glycosidic linkages, and a preferable DP between 2 and 200.
- the fructo-oligosaccharide contains a terminal ⁇ (2,1) glycosidic linked glucose.
- the fructo-oligosaccharide contains at least 7 ⁇ -linked fructose units.
- inulin is used.
- Inulin is a type of fructo-oligosaccharide wherein at least 75% of the glycosidic linkages are ⁇ (2,1) linkages.
- inulin has an average chain length between 8 and 60 monosaccharide units.
- a suitable fructo-oligosaccharide for use in the compositions of the present invention is commercially available under the trade name Raftiline®HP (Orafti).
- Other suitable sources are raftilose (Orafti), fibrulose and fibruline (Cosucra) and Frutafit and frutalose (Sensus).
- the infant or follow on formula comprises a mixture of galactooligosaccharides and fructooligosaccharides in a weight ratio of from 1/99 to 99/1, more preferably from 1/19 to 19/1, even more preferably from 1 to 19/1.
- the infant or follow on formula comprises a mixture of short chain fructooligosaccharides, and long chain fructooligosaccharides in a weight ratio of from 1/99 to 99/1, more preferably from 1/19 to 19/1, even more preferably from 1 to 19/1.
- the infant or follow on formula comprises a mixture of ⁇ 1,3 galactooligosaccharides and ⁇ 1,4 and/or Bl,6 galactooligosaccharides, in a weight ratio of from 1/99 to 99/1, more preferably from 1/19 to 19/1, even more preferably from 1/19 to 1.
- the infant or follow on formula comprises a mixture of transgalacto-oligosaccharide with an average DP below 10, preferably below 6 and a fructo-oligosaccharide with an average DP below 10, preferably below 6.
- the infant or follow on formula comprises a mixture of fructo-oligosaccharide with an average DP below 10, preferably below 6 and a fructo-oligosaccharide with an average DP above 7, preferably above 11, even more preferably above 20.
- transgalacto-oligosaccharide with an average DP below 10, preferably below 6 and a fructo-oligosaccharide with an average DP above 7, preferably above 11, even more preferably above 20.
- the present infant or follow on formula comprises 0.5 to 20 wt.% total non-digestible oligosaccharide, more preferably 1 to 10 wt.%, even more preferably 2 to 10 wt.%, most preferably 2.0 to 7.5 wt.%, based on dry weight of the present infant or follow on formula.
- the present infant or follow on formula when in liquid form, e.g. as a ready-to- feed liquid, preferably comprises 0.1 to 2.5 wt.% non-digestible oligosaccharide, more preferably 0.2 to 1.5 wt.%, even more preferably 0.4 to 1.5 wt.%, based on 100 ml of the present infant or follow on formula.
- Infant formula relates to a nutritional composition for particular nutritional uses by infants during the first months of life and satisfying by themselves the nutritional requirements of such infants until the introduction of appropriate complementary feeding.
- Infant formula is sometimes also referred to as a starter formula or a stage 1 formula.
- an infant formula is used until the infant has reached an age of 4 to 6 months.
- follow on formula relates to a nutritional composition for particular nutritional use by infants when appropriate complementary nutrition is introduced and constituting the principal liquid element in a progressively diversified diet of such infants.
- follow on formula is sometimes also referred to as a stage 2 (infant) formula.
- a follow on formula is used from 4-6 month of age to 12 months of age.
- An infant is a human child with an age of 0 to 12 months.
- the present infant or follow on formula is preferably particularly suitable for providing the complete daily nutritional requirements to a human subject with an age below 12 months, more preferably below 6 months.
- the present nutritional composition is not a yogurt, since yoghurt contains by convention L. bulgaricus (Codex Standard for fermented Milks Codex Stan 243- 2003).
- the present infant or follow on formula comprises a digestible carbohydrate component.
- Preferred digestible carbohydrate components are lactose, glucose, sucrose, fructose, galactose, maltose, starch and maltodextrin. Lactose is the main digestible carbohydrate present in human milk.
- the present infant or follow on formula preferably comprises lactose. As the present infant or follow on formula comprises a composition fermented by lactic acid bacteria, the amount of lactose is reduced compared to its source due to the fermentation whereby lactose is converted into lactate and/or lactic acid. Therefore in the preparation of the present infant or follow on formula lactose is preferably added.
- the present infant or follow on formula does not comprise high amounts of carbohydrates other than lactose.
- lactose Compared to digestible carbohydrates such as maltodextrin, sucrose, glucose, maltose and other digestible carbohydrates with a high glycemic index, lactose has a lower glycemic index and is therefore preferred.
- the present infant or follow on formula preferably comprises digestible carbohydrate, wherein at least 35 wt.%, more preferably at least 50 wt.%, more preferably at least 60 wt.%, more preferably at least 75 wt.%, even more preferably at least 90 wt% , most preferably at least 95 wt% of the digestible carbohydrate is lactose.
- the present infant or follow on formula preferably comprises at least 25 wt.% lactose, preferably at least 40 wt.%, more preferably at least 50 wt% lactose.
- the infant or follow on formula When in liquid form, e.g. as a ready-to-feed liquid, the infant or follow on formula preferably comprises 3.0 to 30 g digestible carbohydrate per 100 ml, more preferably 6.0 to 20, even more preferably 7.0 to 10.0 g per 100 ml.
- the present infant or follow on formula preferably comprises 20 to 80 wt.%, more preferably 40 to 65 wt.% digestible carbohydrates.
- Based on total calories the infant or follow on formula preferably comprises 9 to 14 g digestible carbohydrates per 100 kcal.
- the present infant or follow on formula comprises a lipid component.
- the lipid component of the present infant or follow on formula provides 2.9 to 6.0 g, more preferably 4 to 6 g per 100 kcal of the infant or follow on formula.
- the infant or follow on formula preferably comprises 2.1 to 6.5 g lipid per 100 ml, more preferably 3.0 to 4.0 g per 100 ml.
- the present infant or follow on formula preferably comprises 12.5 to 40 wt.% lipid, more preferably 19 to 30 wt.%.
- the lipid component comprises the essential fatty acids alpha-linolenic acid (ALA), linoleic acid (LA) and preferably long chain polyunsaturated fatty acids (LC-PUFA).
- ALA alpha-linolenic acid
- LA linoleic acid
- LC-PUFA long chain polyunsaturated fatty acids
- the LC-PUFA, LA and/or ALA may be provided as free fatty acids, in triglyceride form, in diglyceride form, in monoglyceride form, in phospholipid form, or as a mixture of one of more of the above.
- the present infant or follow on formula contains at least one, preferably at least two lipid sources selected from the group consisting of rape seed oil (such as colza oil, low erucic acid rape seed oil and canola oil), high oleic sunflower oil, high oleic safflower oil, olive oil, marine oils, microbial oils, coconut oil, palm kernel oil and milk fat.
- the present infant or follow on formula comprises a protein component.
- the protein used in the infant or follow on formula is preferably selected from the group consisting of non-human animal proteins, preferably non-human milk proteins, vegetable proteins, such as preferably soy protein and/or rice protein, and mixtures thereof.
- the present nutritional composition preferably contains casein, and/or whey protein, more preferably bovine whey proteins and/or bovine casein.
- the protein component comprises protein selected from the group consisting of whey protein and casein, preferably both whey protein and casein, and preferably the whey protein and/or casein is from cow's milk.
- the protein comprises less than 5 wt.% based on total protein of free amino acids, dipeptides, tripeptides or hydrolyzed protein.
- the present nutritional composition preferably comprises casein and whey proteins in a weight ratio casein: whey protein of 10:90 to 90: 10, more preferably 20:80 to 80:20, even more preferably 35:65 to 55:45.
- the present infant or follow on formula comprises protein providing 1.8 to 5.5, preferably 1.8 to 3.5 g protein per 100 kcal of the infant or follow on formula, preferably providing 1.8 to 2.5 g per 100 kcal of the infant or follow on formula.
- the infant or follow on formula according to the invention comprises protein preferably in an amount of 2.0 g per 100 kcal or less.
- the infant or follow on formula preferably comprises 0.5 to 6.0 g, more preferably 1.0 to 3.0 g, even more preferably 1.0 to 1.5 g protein per 100 ml, most preferably 1.0 to 1.3 g protein per 100 ml.
- the present infant or follow on formula comprises 5 to 20 wt.% protein, preferably at least 8 wt.% protein based on dry weight of the infant or follow on formula, more preferably 8 to 14 wt.%, even more preferably 8 to 9.5 wt.% protein based on dry weight of the infant or follow on formula.
- the wt.% protein based on dry weight of the present infant or follow on formula is calculated according to the Kjeldahl-method by measuring total nitrogen and using a conversion factor of 6.38 in case of casein, or a conversion factor of 6.25 for other proteins than casein.
- the term 'protein' or 'protein component' as used in the present invention refers to the sum of proteins, peptides and free amino acids.
- the lipid component provides 2.9 to 6 g lipid per 100 kcal, preferably the protein component provides 1.8 to 5.5 g per 100 kcal, preferably 1.8 to 2.5 g per 100 kcal and preferably the digestible carbohydrate component provides 9 to 14 g per 100 kcal, of the final infant or follow on formula, wherein preferably the digestible carbohydrate component comprises at least 60 wt.% lactose based on total digestible carbohydrate, more preferably at least 75 wt.%, even more preferably at least 90 wt.% lactose based on total digestible carbohydrate.
- the amount of total calories is determined by the sum of calories derived from protein, lipids, digestible carbohydrates and non digestible oligosaccharides.
- the infant or follow on formula is in a liquid form.
- the infant or follow on formula is in the form of a powder, preferably a powder suitable for making a liquid ready to drink infant or follow on formula after reconstitution with an aqueous solution, preferably with water.
- the infant or follow on formula is a powder to be reconstituted with water.
- the liquid composition has a viscosity below 100 mPa.s, more preferably below 60 mPa.s, more preferably below 35 mPa.s, even more preferably below 6 mPa.s as measured in a Brookfield viscometer at 20°C at a shear rate of 100 s "1 .
- the nutritional composition has a viscosity when administered close to that of human milk.
- the nutritional composition does not comprise a thickener, preferably it does not comprise a thickener selected form the group consisting of locust bean gum, tara gum, gum tragacanth, guar gum, and fenugreek gum, preferably it does not comprise any of locust bean gum, tara gum, gum tragacanth, guar gum or fenugreek gum.
- the infant or follow on formula preferably comprises 60 to 85, more preferably 60 to 70 kcal/100 ml liquid.
- This caloric density ensures an optimal ratio between water and calorie consumption.
- the osmolarity of the present composition is preferably between 150 and 420 mOsmol/1, more preferably 260 to 320 mOsmol/1.
- the low osmolarity aims to further reduce the gastrointestinal stress.
- the infant or follow on formula is in a liquid form, the preferred volume administered on a daily basis is in the range of about 80 to 2500 ml, more preferably about 200 to 1200 ml per day.
- the number of feedings per day is between 1 and 10, preferably between 3 and 8.
- the infant or follow on formula is administered daily for a period of at least 2 days, preferably for a period of at least 7 days, preferably for a period of at least 2 weeks, preferably for a period of at least 4 weeks, preferably for a period of at least 8 weeks, more preferably for a period of at least 12 weeks, in a liquid form wherein the total volume administered daily is between 200 ml and 1200 ml and wherein the number of feedings per day is between 1 and 10.
- the present inventors found that when a partly fermented infant formula comprising non digestible oligosaccharides was administered to infants, the incidence of colics and the number of crying episodes per day was statistically significantly reduced compared to the study groups of infants to whom partly fermented infant formula without non digestible oligosaccharides were given, or when non-fermented infant formula with non digestible oligosaccharides were given. Also it was found that the duration of crying was statistically significantly decreased compared to the study group of infants receiving non-fermented infant formula without non digestible oligosaccharides .
- the present infant or follow on formula is used for providing nutrition to an infant, a human subject with an age of 0 to 12 months.
- the present infant or follow on formula is preferably orally administered.
- the present formula is preferably an infant formula and/or the formula is used for providing nutrition to a human subject with an age of 0 to 6 months, more preferably 0 to 4 months.
- Colics are defined as a duration of crying for more than three hours a day, for at least three days a week.
- Reduction in colics is to be understood as a reduction in colics in an infant administered with the infant or follow on formula according to the invention, compared to an infant administered with an infant or follow on formula containing only a partly fermented infant formula without non- digestible oligosaccharides as defined herein or compared to an infant administered with a non- fermented infant formula that contained non-digestible oligosaccharides as defined herein.
- Reduced number of crying episodes per day is to be understood as a lower absolute number of crying episodes per day in an infant administered with the infant or follow on formula according to the invention, compared to an infant administered with an infant or follow on formula containing only a partly fermented infant formula without non-digestible oligosaccharides as defined herein or compared to an infant administered with a non-fermented infant formula that contained non-digestible oligosaccharides as defined herein.
- Episodes of crying per day is the same as number of crying times per day.
- Duration of crying refers to the length in time of a crying episode.
- the present invention concerns reducing the incidence of colics in an infant and reducing the number of crying episodes per day in an infant by administering an infant formula or follow on formula comprising a fermented ingredient and non-digestible oligosaccharides as defined herein above. In one embodiment, the present invention concerns reducing the incidence of colics in an infant and decreasing the duration of crying in an infant by administering an infant formula or follow on formula comprising a fermented ingredient and non-digestible oligosaccharides as defined herein above.
- the present invention concerns reducing the number of crying episodes per day in an infant and decreasing the duration of crying in an infant by administering an infant formula or follow on formula comprising a fermented ingredient and non-digestible oligosaccharides as defined herein above. In one embodiment, the present invention concerns reducing the incidence of colics in an infant and reducing the number of crying episodes per day in an infant and decreasing the duration of crying in an infant by administering an infant formula or follow on formula comprising a fermented ingredient and non-digestible oligosaccharides as defined herein above.
- test diet 1 containing 50% LactofidusTM and a specific mixture of 90% short-chain galacto-oligosaccharides (scGOS) and 10% long-chain fructo-oligosaccharides (lcFOS) or test diet 2 containing 15% LactofidusTM and the specific mixture of 90% scGOS and 10% lcFOS or control diet 3 containing only 50% LactofidusTM or control diet 4 containing only the specific mixture of 90% scGOS and 10% lcFOS. All products were produced by Danone, Steenvoorde, France.
- Diet 1 Infant formula 1 comprising per 100 ml 66 kcal, 1.35 g protein (bovine whey protein/casein in 1/1 weight ratio), 8.2 g digestible carbohydrate (of which 5.6 g lactose, and 2.1 g maltodextrin), 3.0 g fat (mainly vegetable fat), 0.8 g non-digestible oligosaccharides of scGOS (source Vivinal® GOS) and IcFOS (source RaftilinHP®) in a 9: 1 wt ratio.
- scGOS source Vivinal® GOS
- IcFOS source RaftilinHP®
- LactofidusTM a commercially available infant formula marketed under brand name Gallia.
- LactofidusTM is a 100 % fermented milk derived composition and is produced by fermenting with S. thermophilus and comprises B. breve. A heat treatment is employed to inactivate the lactic acid bacteria.
- the infant formula comprises about 0.55 wt.% lactic acid + lactate based on dry weight, of which at least 95% is L- lactic acid/lactate.
- the composition further comprises vitamins, minerals, trace elements and other micronutrients according to international directive 2006/141/EC for infant formula.
- Diet 3 Infant formula 3 similar to infant formula 1, but without the 0.8 g non-digestible oligosaccharides of scGOS (source Vivinal® GOS) and IcFOS (source RaftilinHP®) in a 9: 1 wt ratio.
- Diet 4 Infant formula 4 a non fermented infant formula with 0.8 g non-digestible oligosaccharides of scGOS (source Vivinal® GOS) and IcFOS (source RaftilinHP®) in a 9: 1 wt ratio, and for the remainder with similar composition as infant formula 1. Results:
- the number of crying episodes was also lower compared to the group fed with diet 4 (0% LactofidusTM with scGOS/lcFOS). Diet 2 (15% LactofidusTM with scGOS/lcFOS) was less effective in reducing number of crying episodes per day than diet 1.
- N is the total number of infants in the intention to treat group
- n is number of infants contributing to analysis Diary was included in analysis if there were at least 3 days recorded.
- Statistical relevance was calculated using Wilcoxon rank sum test; [a] vs. diet 4; [b] vs. diet 3; [c] vs. diet 2.
- N is the total number of the infants in the ITT group, n is number of infants contributing to analysis. Colics are defined as 'Yes' if infant has a crying duration of at least 3 hours per day for at least 3 days a week. Baseline is not included, because there was no 7 day diary at baseline. Diary was included in analysis if there were at least 3 days recorded. P-value was calculated using Chi-square test: [a] vs. diet 4; [b] vs. diet 3; [c] vs. diet 2.
- Parents completed standardized diaries to assess crying duration. For every subject the crying duration per day (h/day) was calculated.
- Excessive crying was defined as a crying duration > 3 h/day.
- the incidence of colics was defined based on the parent reported duration of crying in the diaries. Colics were defined as "YES" (present), according to adapted Wessel criteria if the children had for at least 3 days within a period of 7 days a duration of crying for 3 hours per day. The analysis was performed by using repeated measures logistic regression.
- Diet 5 Infant formula comprising per 100 ml 66 kcal, 1.2 g protein (bovine whey protein/casein in 1/1 weight ratio), 7.7 g digestible carbohydrate (of which 7.6 g lactose), 3.4 g fat (mainly vegetable fat), 0.8 g non-digestible oligosaccharides of scGOS (source Vivinal® GOS) and lcFOS (source RaftilinHP®) in a 9: 1 wt ratio.
- scGOS source Vivinal® GOS
- lcFOS source RaftilinHP®
- the infant formula 30% based on dry weight was derived from LactofidusTM.
- the infant formula comprised about 0.33 wt.% lactic acid + lactate based on dry weight, of which at least 95% is L-lactic acid + L-lactate.
- the composition further comprised vitamins, minerals, trace elements and other micronutrients according to international directive 2006/141/EC for infant formula.
- Diet 6 Infant formula, similar to infant formula of diet 5, but did not comprise LactofidusTM and no added non-digestible oligosaccharides of scGOS and lcFOS. Results:
- n is number of infants contributing to analysis Furthermore in the AST population infantile colics registered by the investigator as adverse effect was significantly lower in the infants consuming diet 5 (1 out of 94 infants, 1.1%) compared to infants consuming diet 6 (9 out of 104 infants, 8.7%) (p-value 0.020).
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Pediatric Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Dairy Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NZ719161A NZ719161B2 (en) | 2013-11-04 | 2014-11-04 | Fermented formula with non digestible oligosaccharides |
CN201480070402.7A CN105848499A (en) | 2013-11-04 | 2014-11-04 | Fermented formula with non digestible oligosaccharides |
US15/033,543 US20160278414A1 (en) | 2013-11-04 | 2014-11-04 | Fermented formula with non digestible oligosaccharides |
BR112016009357-7A BR112016009357B1 (en) | 2013-11-04 | 2014-11-04 | USES OF A FERMENTED INGREDIENT AND NON-DIGETABLE OLIGOSACCHARIDES IN THE MANUFACTURING OF A FORMULA FOR NEWBORN FORMULA OR CONTINUED FOR USE IN REDUCING THE INCIDENCE OF COLIC IN A NEWBORN |
RU2016122044A RU2663065C2 (en) | 2013-11-04 | 2014-11-04 | Fermented infant formula with indigestible oligosaccharides |
EP14800158.9A EP3079502A1 (en) | 2013-11-04 | 2014-11-04 | Fermented formula with non digestible oligosaccharides |
CN202311103675.4A CN116998729A (en) | 2013-11-04 | 2014-11-04 | Fermentation formulations containing non-digestible oligosaccharides |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP13191392 | 2013-11-04 | ||
EP13191392.3 | 2013-11-04 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2015065194A1 true WO2015065194A1 (en) | 2015-05-07 |
Family
ID=49513867
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/NL2014/050762 WO2015065194A1 (en) | 2013-11-04 | 2014-11-04 | Fermented formula with non digestible oligosaccharides |
Country Status (6)
Country | Link |
---|---|
US (1) | US20160278414A1 (en) |
EP (1) | EP3079502A1 (en) |
CN (2) | CN105848499A (en) |
BR (1) | BR112016009357B1 (en) |
RU (1) | RU2663065C2 (en) |
WO (1) | WO2015065194A1 (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017194607A1 (en) * | 2016-05-10 | 2017-11-16 | N.V. Nutricia | Fermented infant formula |
WO2017194615A1 (en) * | 2016-05-10 | 2017-11-16 | N.V. Nutricia | Fermented infant formula |
WO2020161113A1 (en) | 2019-02-04 | 2020-08-13 | N.V. Nutricia | Fermented formula with non digestible oligosaccharides for sleep improvement |
US20210346434A1 (en) * | 2019-01-16 | 2021-11-11 | N.V. Nutricia | Fermented formula with non-digestible oligosaccharides for use in rotavirus induced infection |
US11179427B2 (en) | 2013-01-21 | 2021-11-23 | Eth Zurich | Baby food composition comprising viable propionic acid-producing bacteria |
US20220079962A1 (en) * | 2019-06-04 | 2022-03-17 | N.V. Nutricia | Nutritional composition comprising 2'fucosyllactose and dietary butyrate |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20180133287A1 (en) * | 2016-11-14 | 2018-05-17 | Mead Johnson Nutrition Company | Nutritional compositions providing dietary management of colic |
EP3749328A1 (en) * | 2018-02-09 | 2020-12-16 | N.V. Nutricia | Fermented formula with non-digestible oligosaccharides |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2723960A1 (en) | 1994-08-31 | 1996-03-01 | Gervais Danone Co | Prodn. of Streptococcus thermophilus cultures rich in beta-galactosidase |
EP0778885A1 (en) | 1994-08-31 | 1997-06-18 | Compagnie Gervais-Danone | Preparation of galacto-oligosaccharide and beta-galactosidase enriched products fermented using streptococcus thermophilus |
EP1615657A1 (en) | 2003-04-16 | 2006-01-18 | Compagnie Gervais-Danone | Immunomodulatory product obtained from a bifidobacterium culture and compositions containing the same |
EP1685763A1 (en) | 2004-05-17 | 2006-08-02 | N.V. Nutricia | Synergism of GOS and polyfructose |
WO2010008278A1 (en) | 2008-07-15 | 2010-01-21 | N.V. Nutricia | Treatment of gut motility disorders |
EP2520181A1 (en) | 2011-05-02 | 2012-11-07 | N.V. Nutricia | Fermented infant formula |
US20130189398A1 (en) * | 2011-07-26 | 2013-07-25 | Nucitec S.A. De C.V. | Nutritional Composition for Children with Reflux, Colic and/or Constipation |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008153377A1 (en) * | 2007-06-15 | 2008-12-18 | N.V. Nutricia | Nutrition with non-viable bifidobacterium and non-digestible oligosaccharide |
WO2012078039A1 (en) * | 2010-12-06 | 2012-06-14 | N.V. Nutricia | Fermented infant formula |
GB2511993B (en) * | 2011-11-30 | 2019-09-18 | Ritter Pharmaceuticals Inc | Prebiotic formulations and methods of use |
-
2014
- 2014-11-04 US US15/033,543 patent/US20160278414A1/en active Pending
- 2014-11-04 EP EP14800158.9A patent/EP3079502A1/en active Pending
- 2014-11-04 RU RU2016122044A patent/RU2663065C2/en active
- 2014-11-04 BR BR112016009357-7A patent/BR112016009357B1/en active IP Right Grant
- 2014-11-04 CN CN201480070402.7A patent/CN105848499A/en active Pending
- 2014-11-04 WO PCT/NL2014/050762 patent/WO2015065194A1/en active Application Filing
- 2014-11-04 CN CN202311103675.4A patent/CN116998729A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2723960A1 (en) | 1994-08-31 | 1996-03-01 | Gervais Danone Co | Prodn. of Streptococcus thermophilus cultures rich in beta-galactosidase |
EP0778885A1 (en) | 1994-08-31 | 1997-06-18 | Compagnie Gervais-Danone | Preparation of galacto-oligosaccharide and beta-galactosidase enriched products fermented using streptococcus thermophilus |
EP1615657A1 (en) | 2003-04-16 | 2006-01-18 | Compagnie Gervais-Danone | Immunomodulatory product obtained from a bifidobacterium culture and compositions containing the same |
EP1685763A1 (en) | 2004-05-17 | 2006-08-02 | N.V. Nutricia | Synergism of GOS and polyfructose |
WO2010008278A1 (en) | 2008-07-15 | 2010-01-21 | N.V. Nutricia | Treatment of gut motility disorders |
EP2520181A1 (en) | 2011-05-02 | 2012-11-07 | N.V. Nutricia | Fermented infant formula |
US20130189398A1 (en) * | 2011-07-26 | 2013-07-25 | Nucitec S.A. De C.V. | Nutritional Composition for Children with Reflux, Colic and/or Constipation |
Non-Patent Citations (3)
Title |
---|
L. PROSKY ET AL., J. ASSOC. ANAL. CHEM, vol. 71, 1988, pages 1017 - 1023 |
SAVINO ET AL., EUR J CLIN NUTR, vol. 60, 2006, pages 1304 - 1310 |
SAVINO F ET AL: "Reduction ofcrying episodes owing the infantile colic: a randomized controlled study on the efficacy of a new infant formula", EUROPEAN JOURNAL OF CLINICAL NUTRITION, NATURE PUBLISHING GROUP, GB, vol. 60, 1 January 2006 (2006-01-01), pages 1304 - 1310, XP002623529, ISSN: 0954-3007, [retrieved on 20060531], DOI: 10.1038/SJ.EJCN.1602457 * |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11179427B2 (en) | 2013-01-21 | 2021-11-23 | Eth Zurich | Baby food composition comprising viable propionic acid-producing bacteria |
WO2017194607A1 (en) * | 2016-05-10 | 2017-11-16 | N.V. Nutricia | Fermented infant formula |
WO2017194615A1 (en) * | 2016-05-10 | 2017-11-16 | N.V. Nutricia | Fermented infant formula |
CN109688839A (en) * | 2016-05-10 | 2019-04-26 | N·V·努特里奇亚 | The infant formula of fermentation |
EP3454672B1 (en) | 2016-05-10 | 2020-07-01 | N.V. Nutricia | Uses of fermented infant formula |
EP3841892A1 (en) * | 2016-05-10 | 2021-06-30 | N.V. Nutricia | Fermented infant formula |
US20210346434A1 (en) * | 2019-01-16 | 2021-11-11 | N.V. Nutricia | Fermented formula with non-digestible oligosaccharides for use in rotavirus induced infection |
WO2020161113A1 (en) | 2019-02-04 | 2020-08-13 | N.V. Nutricia | Fermented formula with non digestible oligosaccharides for sleep improvement |
US20220079962A1 (en) * | 2019-06-04 | 2022-03-17 | N.V. Nutricia | Nutritional composition comprising 2'fucosyllactose and dietary butyrate |
Also Published As
Publication number | Publication date |
---|---|
BR112016009357A2 (en) | 2017-08-01 |
NZ719161A (en) | 2021-02-26 |
RU2016122044A (en) | 2017-12-11 |
CN116998729A (en) | 2023-11-07 |
EP3079502A1 (en) | 2016-10-19 |
RU2016122044A3 (en) | 2018-05-30 |
RU2663065C2 (en) | 2018-08-01 |
CN105848499A (en) | 2016-08-10 |
US20160278414A1 (en) | 2016-09-29 |
BR112016009357B1 (en) | 2021-09-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9596876B2 (en) | Method for stimulating the intestinal flora | |
US9717270B2 (en) | Fermented infant formula with non digestible oligosaccharides | |
RU2663065C2 (en) | Fermented infant formula with indigestible oligosaccharides | |
EP2983522B1 (en) | Fermented nutrition with non-digestible oligosaccharides with increased iron bioavailability | |
EP2117355A1 (en) | Method of improving skills with a composition comprising non-digestible saccharide | |
EP2983482B1 (en) | Fermented nutrition high in lactose with increased iron bioavailability | |
EP3397076A1 (en) | Fermented formula with non-digestible oligosaccharides | |
WO2020161113A1 (en) | Fermented formula with non digestible oligosaccharides for sleep improvement | |
CN109688839A (en) | The infant formula of fermentation | |
CN109475165A (en) | The infant formula of fermentation | |
NZ719161B2 (en) | Fermented formula with non digestible oligosaccharides | |
WO2020229689A1 (en) | Fermented formula for improving intestinal development |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 14800158 Country of ref document: EP Kind code of ref document: A1 |
|
REEP | Request for entry into the european phase |
Ref document number: 2014800158 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2014800158 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: IDP00201602848 Country of ref document: ID |
|
WWE | Wipo information: entry into national phase |
Ref document number: 15033543 Country of ref document: US |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112016009357 Country of ref document: BR |
|
ENP | Entry into the national phase |
Ref document number: 2016122044 Country of ref document: RU Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 112016009357 Country of ref document: BR Kind code of ref document: A2 Effective date: 20160427 |