WO2014159080A1 - Methods for determining active ingredients of an herbal medicine, sources of and catalytic pathways for production thereof - Google Patents
Methods for determining active ingredients of an herbal medicine, sources of and catalytic pathways for production thereof Download PDFInfo
- Publication number
- WO2014159080A1 WO2014159080A1 PCT/US2014/021885 US2014021885W WO2014159080A1 WO 2014159080 A1 WO2014159080 A1 WO 2014159080A1 US 2014021885 W US2014021885 W US 2014021885W WO 2014159080 A1 WO2014159080 A1 WO 2014159080A1
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- WO
- WIPO (PCT)
- Prior art keywords
- mineral
- herbal medicine
- liquor
- medicinal
- minerals
- Prior art date
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
Definitions
- the presently disclosed and claimed inventive process(es), procedure(s), method(s), product(s), result(s) and/or concept(s) (collectively hereinafter referenced to as the "presently disclosed and claimed inventive concept(s)") relates generally to herbal medicine prescriptions, and more particularly to methods for determining active ingredients of such herbal medicines including identifying organic sources and catalytic pathways to produce such active ingredients.
- Herbs have been utilized for centuries for healing ailments ranging from arthritis to tuberculosis.
- Prescriptions of herbal medicines typically include a combination of herbs which together form an herbal medicine prescription, but such can include only one herb.
- Herbal medicine prescriptions are typically composed of lignocellulosic species, often in whole or in part, such as stems, roots, leaves, and seeds, of herbal grasses and shrubs, occasionally whole fruits or skins of plants, and in rare cases animal components such as armor or crust.
- Herbal medicine prescriptions have a long history of development in many parts of the world. Many prescriptions have been kept as family trade secrets. Such prescriptions remain more of an art than a science and their effectiveness has been mixed from case to case.
- Herbal decoction is typically in boiling water and has traditionally been practiced in sandy pots by individual patients. The decoction process receives little control in the quality of water, pH, and the boiling temperature (as a result of varied mineral content). Decoction is usually performed in open air.
- a method comprises: a) utilizing an herbal medicine comprising at least one herb comprising cellulosic material and minerals, wherein the cellulosic material comprises organic components, which include polysaccharides, lignin, peptides, alkaloids, and proteins; b) analyzing each of the herbs of the herbal medicine to identify and quantify an analyte selected from the group consisting of: i) the minerals, ii) the elements: C, H, N, O, S, B, P, and halides, iii) the organic components, and iv) combinations thereof;
- step j comparing the medicinal efficacies of the liquors tested in step j) to determine significant differences in medicinal efficacies and identifying liquors demonstrating significant differences in medicinal efficacy as effective liquors.
- a method of determining active ingredients of an herbal medicine comprises:
- cellulosic material comprises organic components, which include polysaccharides, lignin, peptides, alkaloids, and proteins;
- herbal medicine prescriptions can comprise at least one herb comprising cellulosic material and minerals, wherein the cellulosic material can comprise organic components.
- the organic components can be selected from the group consisting of carbohydrates; polysaccharides; lignin; peptides; proteins; extractives such as steroids, fatty acids, fatty alcohols, alkylfrulates, terpenoids, phenolics, and alkaloids; and combinations thereof; and the organic components as a whole comprise elements C and H; and can further comprise at least one additional element selected from the group consisting of N, O, S, B, P, halides (such as F, CI, Br, and I), and combinations thereof.
- the herbal medicine can be a prescription comprising a combination of individual herb species in prescribed weight for each in the prescription, or it can be a single herb.
- An embodiment comprises the following steps:
- each of the herbs of the herbal medicine to identify and quantify an analyte selected from the group consisting of: i) the minerals, ii) the elements: C, H, N, O, S, B, P, and halides, iii) the organic components, and iv) combinations thereof;
- step j comparing the medicinal efficacies of the liquors tested in step j) to determine significant differences in medicinal efficacies and identifying liquors demonstrating significant differences in medicinal efficacy as effective liquors.
- Suitable conditions in step c) can include a temperature in the range of from about 0 to about 250°C, or about 50 to about 150°C, or about 80 to about 120°C; and a pH in the range of from about 0.1 to about 14, or from about 3 to about 10, or from about 5 to about 9.
- the solvent can be selected from the group consisting of water, an alcohol, an organic acid, an ester, a lactone, and combinations thereof, and the aqueous solution can further comprise a polar solvent, which can comprise an alcohol, an organic acid, an ester, a lactone, and combinations thereof.
- the above described method of this embodiment can further comprise:
- step c) combining an additive mineral comprising at least one mineral selected from the group consisting of the minerals removed in step c) to a portion of the herbal medicine to form a mineral-enhanced herbal medicine;
- step j comparing the medicinal efficacy of the mineral-enhanced herbal medicine liquor with the medicinal efficacies of the liquors tested in step j) to determine significant differences in medicinal efficacies and, if the medicinal efficacy of the mineral-enhanced herbal medicine liquor is significantly enhanced over the medicinal efficacies of the liquors tested in step j), identifying the mineral-enhanced herbal medicine liquor as an effective liquor and identifying the additive mineral as an active mineral;
- the above described method of this embodiment can further comprise: q) combining an additive mineral comprising at least one mineral selected from the group consisting of the non-removable minerals to a portion of the herbal medicine to form a mineral-enhanced herbal medicine; r) decocting the mineral-enhanced herbal medicine in a portion of the aqueous solution to form a mineral-enhanced herbal medicine liquor;
- the above described method of this embodiment can further comprise v) extracting and analyzing potential medicinal ingredients from each of the effective liquors to identify and quantify the potential medicinal ingredients;
- step w) comparing the results of the clinical testing in step w) for each of the potential medicinal ingredients and for each combination of the potential medicinal ingredients and identifying any of the potential medicinal ingredients or combinations of the potential medicinal ingredients which are effective as an effective medicinal ingredient or as an effective medicinal ingredient combination.
- a method of determining active ingredients of an herbal medicine comprises:
- the above described method of this embodiment can further comprise: j) analyzing a portion of the herbal medicine to identify and quantify the minerals resulting in a list of identified minerals;
- the decocting steps described above can be performed at any suitable conditions to accomplish decocting.
- suitable conditions for decocting can include a temperature in the range of from about 0 to about 250°C, or about 50 to about 150°C, or about 80 to about 120°C; and a pH in the range of from about 0.1 to about 14, or from about 3 to about 10, or from about 5 to about 9.
- suitable conditions for decocting can include temperature and pressure conditions sufficient to boil the aqueous solution. The pressure can exceed ambient.
- the conventional herbal medicine decoction is typically carried out at local ambient pressure to the boiling point of the aqueous solution.
- the liquors formed above from decoction of the herbal medicines can be separated from the residues of the herbal medicines by any separation method known to separate a liquid from a solid, and more specifically, by a method selected from the group consisting of filtration, decanting, pressurized filtration, and combinations thereof.
- minerals can be left in the medicinal liquor. These minerals can be optionally removed by various methods from the liquors.
- the minerals or mineral ions can be removed by a method selected from the group consisting of ion exchange with protonic polymeric resins, precipitation with the aid of precipitating reagents (such as for heavy metals), chelation using chelating reagents, and combinations thereof.
- the precipitating reagents can include, but are not limited to, carbon dioxide, alkali carbonates, etc.
- the above described method of this embodiment can further comprise: p) analyzing a portion of the herbal medicine to identify and quantify the organic components; q) isolating each of the organic components from a portion of the herbal medicine;
- Applicant believes that certain mineral elements in an herbal medicine prescription, containing at least one herb and usually a mix of multiple herbs, catalyze the transformation of natural components present in the herbs during the decoction process to produce new molecules that are medicinally active for the treatment of certain diseases.
- Applicant believes that the natural components of herbs undergo a catalytic transformation process to produce new molecules, some of which are active medicinal ingredients, due to the presence of certain minerals embedded in the mass of the herbs.
- the catalyzed transformation could be the re-arrangement of certain bonds in a natural component of a single herb or the elimination of a certain functional group(s) in a natural component of a single herb, or the condensation of two or multiple components derived from different herb species.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Medicines Containing Plant Substances (AREA)
- Sampling And Sample Adjustment (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201480022788.4A CN105188724A (en) | 2013-03-14 | 2014-03-07 | Methods for determining active ingredients of an herbal medicine, sources of and catalytic pathways for production thereof |
EP14775446.9A EP2968425A4 (en) | 2013-03-14 | 2014-03-07 | Methods for determining active ingredients of an herbal medicine, sources of and catalytic pathways for production thereof |
US14/776,416 US20160033464A1 (en) | 2013-03-14 | 2014-03-07 | Methods for determining active ingredients of an herbal medicine, sources of and catalytic pathways for production thereof |
CA2905222A CA2905222A1 (en) | 2013-03-14 | 2014-03-07 | Methods for determining active ingredients of an herbal medicine, sources of and catalytic pathways for production thereof |
JP2016500872A JP2016512502A (en) | 2013-03-14 | 2014-03-07 | Methods for determining the active ingredients of herbal medicines, their sources, and catalytic pathways for their production |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361784112P | 2013-03-14 | 2013-03-14 | |
US61/784,112 | 2013-03-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2014159080A1 true WO2014159080A1 (en) | 2014-10-02 |
Family
ID=51625104
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2014/021885 WO2014159080A1 (en) | 2013-03-14 | 2014-03-07 | Methods for determining active ingredients of an herbal medicine, sources of and catalytic pathways for production thereof |
Country Status (6)
Country | Link |
---|---|
US (1) | US20160033464A1 (en) |
EP (1) | EP2968425A4 (en) |
JP (2) | JP2016512502A (en) |
CN (1) | CN105188724A (en) |
CA (1) | CA2905222A1 (en) |
WO (1) | WO2014159080A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3553517A1 (en) * | 2018-04-14 | 2019-10-16 | Jianyi Zhang | Method of identifying bioactive compounds in herbals |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030219498A1 (en) * | 2002-05-21 | 2003-11-27 | Li-Wei Hsu | Method to ascertain the quality of herbs |
US20070009961A1 (en) * | 2005-07-07 | 2007-01-11 | Academia Sinica | Identification of targeting component of herbal medicines from simplified HPLC spectrum using after flowing through immobilized receptor (AFTIR) method |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2301860C (en) * | 1997-08-28 | 2009-05-26 | Cv Technologies Inc. | Chemical and pharmacological standardization of herbal extracts |
CN100356380C (en) * | 2001-02-13 | 2007-12-19 | 科学与工业研究会 | Method of chromatogram fingerprint atlas, single medicine and preparation standardization |
EP2311475B1 (en) * | 2002-08-14 | 2016-08-31 | GW Pharma Limited | Extraction of pharmaceutically active cannabinoids from plant materials |
-
2014
- 2014-03-07 EP EP14775446.9A patent/EP2968425A4/en not_active Withdrawn
- 2014-03-07 JP JP2016500872A patent/JP2016512502A/en active Pending
- 2014-03-07 US US14/776,416 patent/US20160033464A1/en not_active Abandoned
- 2014-03-07 WO PCT/US2014/021885 patent/WO2014159080A1/en active Application Filing
- 2014-03-07 CN CN201480022788.4A patent/CN105188724A/en active Pending
- 2014-03-07 CA CA2905222A patent/CA2905222A1/en not_active Abandoned
-
2016
- 2016-12-01 JP JP2016234586A patent/JP2017071630A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030219498A1 (en) * | 2002-05-21 | 2003-11-27 | Li-Wei Hsu | Method to ascertain the quality of herbs |
US20070009961A1 (en) * | 2005-07-07 | 2007-01-11 | Academia Sinica | Identification of targeting component of herbal medicines from simplified HPLC spectrum using after flowing through immobilized receptor (AFTIR) method |
Non-Patent Citations (4)
Title |
---|
CHENG, Y. ET AL.: "A causal relationship discovery-based approach to identifying active components of herbal medicine", COMPUTATIONAL BIOLOGY AND CHEMISTRY, vol. 30, 2006, pages 148 - 154, XP024954016 * |
LIANG, Y.-Z. ET AL.: "Quality control of herbal medicines", JOURNAL OF CHROMATOGRAPHY B, vol. 812, 2004, pages 53 - 70, XP004648209 * |
See also references of EP2968425A4 * |
YANG, D.-Z. ET AL.: "Development of a novel method combining HPLC fingerprint and multi-ingredients quantitative analysis for quality evaluation of traditional Chinese medicine preparation", TALANTA, vol. 85, 2011, pages 885 - 890, XP028097036 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3553517A1 (en) * | 2018-04-14 | 2019-10-16 | Jianyi Zhang | Method of identifying bioactive compounds in herbals |
Also Published As
Publication number | Publication date |
---|---|
JP2016512502A (en) | 2016-04-28 |
US20160033464A1 (en) | 2016-02-04 |
EP2968425A4 (en) | 2016-12-07 |
JP2017071630A (en) | 2017-04-13 |
CN105188724A (en) | 2015-12-23 |
CA2905222A1 (en) | 2014-10-02 |
EP2968425A1 (en) | 2016-01-20 |
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