WO2007147085A2 - Treatment of wound using aganocides and negative pressure - Google Patents
Treatment of wound using aganocides and negative pressure Download PDFInfo
- Publication number
- WO2007147085A2 WO2007147085A2 PCT/US2007/071263 US2007071263W WO2007147085A2 WO 2007147085 A2 WO2007147085 A2 WO 2007147085A2 US 2007071263 W US2007071263 W US 2007071263W WO 2007147085 A2 WO2007147085 A2 WO 2007147085A2
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- WIPO (PCT)
- Prior art keywords
- wound
- treatment
- aganocide
- reduced pressure
- application
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/20—Elemental chlorine; Inorganic compounds releasing chlorine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/90—Negative pressure wound therapy devices, i.e. devices for applying suction to a wound to promote healing, e.g. including a vacuum dressing
- A61M1/91—Suction aspects of the dressing
- A61M1/916—Suction aspects of the dressing specially adapted for deep wounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/90—Negative pressure wound therapy devices, i.e. devices for applying suction to a wound to promote healing, e.g. including a vacuum dressing
- A61M1/92—Negative pressure wound therapy devices, i.e. devices for applying suction to a wound to promote healing, e.g. including a vacuum dressing with liquid supply means
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/90—Negative pressure wound therapy devices, i.e. devices for applying suction to a wound to promote healing, e.g. including a vacuum dressing
- A61M1/96—Suction control thereof
- A61M1/962—Suction control thereof having pumping means on the suction site, e.g. miniature pump on dressing or dressing capable of exerting suction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M27/00—Drainage appliance for wounds or the like, i.e. wound drains, implanted drains
Definitions
- a bacterid count of 1 ty or more per grain of tissue is defined us infected.
- Ii H known that wound (issue ihat is infected ⁇ ⁇ not be si ⁇ cepfeble io wound closure. Therefore, a number of approaches have been taken to reduce bacterial infection, for example, iodine or silver salts have beer?
- Dakm's solution a buffered solution of NaOC 1 of a mild basic pH
- organic or inorganic aganocides have been proposed such as
- HMMMl Ii is understood that any aspect or feature of the present invention whether characterized as preferred or not characterized as preferred may be combined with any other aspect or feature of the invention, whether such other feature is characterized as preferred or not characterized as preferred.
- a feature described as preferred. for example a pH range, or a specific pH for a particular" composition may be combined with another not preferred feature, for example, a NaCl concentration of 1 ,2%, without deviating from the present invention.
- Aganocide refers to N-halo or N.N-dihalo amino acids, or
- N-halo- or N,N-dihaio amino acid sources See “N-HaIo or N,N ⁇ Dihalo Amino Acid
- fOO ⁇ Sf Atraumatic wiping refers to removal of undesirable matter from or around the wound or skin around the wound without causing trauma cither to newly formed tissue or the skin around the wound.
- Bacterial load refers to the bacterial count in a wound determined by wound biopsy.
- Biopsy refers Io a method of assessing the infection of a wound or ulcer.
- I ! J ''Cell-compatible composition is an aganocide composition or a NaC! containing composition with acceptable cytotoxicity and is physiologically acceptable for therapeutic applications.
- Combination treatment regimen is a treatment regimen applying vacuum- assisted wound treatment with use of an aganocide as described herein.
- Delivery refers to the removal of tissue ia and around the wound that may negatively affect wound healing or closure.
- K-HaIo or N.N-dihalo ammo acid source refers to a composition that releases an N-haio- or N,N-dihalo amino acid for example, a salt, such as the sodium, potassium, magnesium or calcium salt, that, when treated with an acid releases the conespondmg TN-halo- or N,N-Dihalo ⁇ nu ' no Acid.
- a salt such as the sodium, potassium, magnesium or calcium salt
- N JN-dihak> amino acid source may be a polymeric matrix that is stable toward the N-HaIo or N ⁇ ' - diaalo amino acid (or aganocide) but gradually releases the N-HaIo or NLN-dihalo amino acid into the wound,
- Hvp ⁇ chlorous acid source or "HOCl source” refers to a composition that releases, or under certain conditions, is capable of releasing OC)Ci
- NaOCI or KOCl that is treated with an acid releases HOCi.
- rule it wiii be an application for more than 120 minutes, preferably for more than 6 hours.
- Precede refers to a step, procedure or application in the regimens described herein that goes before a step, procedure or application that follows.
- IQQlM] "'Regimen” is a systematic topical therapy plan or schedule designed to promote the healing of wounds or ulcers.
- Removal of a composition refers to the separation of a matter or a composition from the wound, ulcer or skin around the wound, for example, of a component of the regime described.
- Saline is a NaCI containing composition, mostly in form of a solution that us physiological 1 ⁇ acceptable to the mammalian skin in the area of the wound or ulcer; it may also contain other alkali or earth-alkali ions.
- "The size of the wound and/or the bacterial load have been reduced sufficiently" refers to the wound healing progress achieved after application of the regimen herein described to such an extent that the wound-healing would continue even if the regimen would be discontinued.
- Time intervals appropriate to patients in need of wound care refer to the io.ng-tcr.rn application of a component or composition of the regimen beneficial in terms of promoting wound healing, enhancing patient compliance and in a health care facility coincide with patient visits by physicians, nurse practitioners, nurse assistants and personnel capable of performing the wound care required by the various regimens described herein.
- Time kill refers to an in vitro measure of how fast a given antimicrobial can kill test bacteria.
- Wiping is a specific removal process by nibbing or cleaning or drying, in general without causing infection of a wound or ulcer.
- wound closure is occurring refers to the wound healing progress achieved after application of the regimen herein described. In general, such progress is achieved when the fraction of open wounds is reduced by at least 70 % relative to the open woands existing prior to administration of the regimens described herein.
- wound dressing refers to a protective or therapeutic material applied to a wound minimizing infection and producing an environment beneficial to promoting wound healing.
- wound tracing refers to various methods or a specific method to measure and / or evaluate wound surface area, j ⁇ 034
- a substantially non-toxic, non-irritating member of an inorganic sganoeide selected from the group consisting of hypochlorous acid, an inorganic source of hs pochlorous acid and mixtures thereof, bypochiorous acid, an ⁇ noignnic source of hypochlorous acid and mixtures thereof, in conjunction with, or before, during or after applying reduced pressure to the wound.
- an orga ⁇ ic agai ⁇ de composition selected from the group consisting of an N-HaIo amino acid, an VN- ⁇ halo ammo acid, a source of N-HaIo amino acid, a source of N.N-chtialo ammo acic, and mixtures thereof, may also be used m conjunction with, or before, dunng or after apphmg reduced pressure to the wound,
- the method may use an> de ⁇ ice or combination of devices suitable for delivering the organic or the inorganic aganocidc composition ⁇ intermittently to the wound and for a ⁇ pl> mg negative pressure (i.e., vacuum) io the wound.
- Deuces include the one described m US 5,63C ⁇ 643, 5,645,08! or US 7,216,651 or a de ⁇ iee as described in ⁇ n ⁇ ⁇ liorac Surg 2002; 74:15 ⁇ 6-1600, (VAC s>slera provided b> KCI Inc., San Antonio, Texas), or m KCFs Y A.C.
- the inorganic or organic aganocidc composition may also be delh ered to ihe wound before, or after applying the vacuum de ⁇ see or system, or between iwu oi more applications of ihe vaeunrn system to the wound.
- the inorganic or organic aga ⁇ odde composition may be sprayed or poured on the wound or applied by injection, by irrigation, by dripping ⁇ on the wound, or gentle rubbing it onto ihe wound, or touching the wound, or by any form of contact that achiexes its " application onto the wound without negatively affecting ⁇ u)und healing.
- the vacuum system may not he used for agaruvide delivery, but only for application of the vacuum.
- the new treatment method is effective to promote healing of difficult healing v.ouuds.
- the aganocide composition and reduced pressure are applied to a wound for a sufficient time mid with sufH ⁇ em concentration of die aganocide and sufficiently reduced pressure to facilitate wound closure. This can be achieved by many different approaches as described sn more detail below, DFTAILED DESCRIPTION
- necrotic tissue is remov ed and the organic or inorganic aganocide composition applied to the wound.
- the wound ma ⁇ ' b ⁇ traced to determine its surface area. Biopss may also be used to ascertain the degree of infection of tbe wound.
- the aganocide composition may be applied by spraying it cm the wound, b> injection, by pouring it on the wound, by irrigation, by using a soft carrier containing the aganoctde and contact ⁇ or touching the wound lightly with the carrier containing the aganocide.
- a cover such as an impermeable cover is fitted on the wound to cover and eiidose the wound.
- a suitable porous material ma> be placed into or over the wound before the impermeable co ⁇ er is fitted on ihe wound.
- Tbe mip.Tineable cover is configured to maintain reduced pressure at the site of the wound.
- the eo ⁇ er is connected with a vacuum pump to apply a vacuum or reduce pressure to the % ⁇ ur ⁇ i.
- the cover is then sealed, for example, v* ith drape to provide an airtight seal.
- the r eafter suction is applied to collect wound fluid that is removed b> suction.
- the inorganic aganocidc composition should be applied in concentration of up t «j ! O 1 OOO pprn.
- the effective amount of hypochlorous acid is from about 20 to about 500 ppm.
- the effective oxyn is from about 40 to about 250 ppni.
- the effective amount is from about 50 to about 150 ppir.
- the aganocide is.sed is an organic aganocidc composition such as an N- halogenatcd or ⁇ ' ,N-dihalogemned amino acid or a derivative thereof, for example, as described m WO02221 18 W. Gmtardi, U.S. Publication No. 2005 00651 15 A I and U.S. Serial No 1 1 '339,9S "7 filed January 25. 2006, the agano ⁇ de concentration to be chosen shot Id be higher than the inorganic aganocides because the organic agauoci ⁇ es have a mile antibacterial action.
- the mono-lialogcnaied ammo acids i.e.
- N-halo amino acids such as N-ehlorotamine.
- N-ehlorotamine have the mildest antibacterial action among all N-halogenateri amino acids, milder than the antibacterial action of N,N-dichIorota ⁇ rine or N,N-diehloro- 2,2-ditncthyl taurine. Therefore the higher concentration ranges may be used for the N- irjonohnlogeuated amino acids.
- the effective amount of an organic aganocide such as an N- halogenated amino acid is from about 100 to about 10,000 ppm. Even more preferred, ihe effective amount is from about 200 to about 2,QGO ppm. Most preferably, the effective amount it> from about 400 to about J ,000 ppm.
- the pH of the inorganic aganocide compositions which are prefer&biy applied in form of aqueous solutions is not critical as long it is acidic io neutral
- a broad pi I range from about 2 to about 7 may be a useful range.
- a more preferred pH range would be between about 3 and about 7, and a pH range between about 3,5 to about 7 may be most preferred. If an acidic pH range is the best choice for a given wound condition, a pH between about 3,5 to about 7 is preferred.
- Evzn more preferred is a pH in a range of between about 3.5 and about. 6.5.
- the pH of the organic aga ⁇ ocide compositions which are preferably applied in form of aqueous solutions is not critical although a broad pH range from about 2 to about 9.5 may be a useful range. A more preferred pH range would b ⁇ between about 3 and about 9, and a pH range between about 3.5 to about 8.5 may be most preferred. If an acidic pf i range is the best choice for a given wound condition, a pH between about 3.5 to about 7.5 is preferred. Even more preferred may be a range between about 3,5 and about 6.5,
- the inorganic or organic aganocide composition is used in the form of a solution, lhen water or a lower alcohol (such as ethanol or isoproparsol) or mixtures thereof may be the best choice for the vehicle. If an aqueous composition is being used it would be most advantageous to use a saline composition (for example, NaCl) in amounts of about 0.4 to about 1.2 % by weight of NaCi, or the equivalent of a similar salt such as KCK because such compositions would be even less irritating. ( ⁇ 044)
- the method described herein includes at least one step of applying the inorganic or organic aganocide to the wound and its removal or replacement, for example, before application of the vacuum.
- the aganocide may also be applied while the vacuum is on by a device having a separate port for delivering the aganocide composition with a shut-off valve which would initially be in a closed position while the wound is under reduced pressure. Then a valve controlling the port to the vacuum pump would be shut off and the valve controlling delivery of the aganocide composition would be slowly and carefully opened to apply a certain often predetermined amount of aganocide to the wound. After shutting off the aganocide delivery valve, the vacuum can be re-applied, resulting in removal of wound exudate and excess aganocide.
- a preferred embodiment comprises at least two applications of (he inorganic or organic aganocide, or one application of aganocide followed by saline treatment and at leas! one application of reduced pressure, to debride the wound and reduce bacterial court, for example, before the application of the vacuum, and after the vacuum treatment has been discontinued.
- This method would have the benefit that bacterial COUTH of the wound is reduced before negative pressure (vacuum) is applied that will result in an even further reduced bacterial count. After the vacuum has been shut off, further debridement with the aganocide or with saline may be performed.
- Au even more preferred treatment involves an intermittent treatment regimen, for example as follows:
- composition (aganocide or saline); and optionally
- a complete treatment cycle comprises at least two applications of the inorganic or organic aganocide composition and lasts at least 24 hours during which negative pressure is applied.
- the aganocide composition applied to the wound may be removed by changing the dressing, changing the dressing and wiping, delivery of fresh aganocide composition and rinsing off aganocide residues, such as by using water and/or saline solution, from previously applied aganocide or pouring on new aganocide composition and rinsing off aganocide residues from previously applied aganocide.
- a typical treatment regimen will last from at least 1.2 hours and can last up to several weeks and will comprise multiple treatment cycles until the status of sufficient wound closure has been achieved to enable self-healing of the wound. It is preferred to achieve wound closure or the status of self-healing in two weeks or less time.
- the reduced pressure to be applied may range from about 0.01 to about 0.99 atmospheres, more preferably from about 0.03 to about 0.8, or from about 0.03 to about 0,4 atmospheres, A preferred reduced pressure applies at least 0.1 1 or 0.136 aim to the wound. In certain applications, it is beneficial to provide the reduced pressure in an intermittent or cyclic manner which may be achieved by manual or automatic control of the vacuum system. Details of the VAC treatment regimen are described in U.S. Patent No. 5,636,643 and U.S. Patent No. 7,216,651,
- A is hydrogen, HaINIi- or RahN- wherein Hal is halogen selected from the group consisting of cliloro and bromo; R is a carbon carbon single bond or a divalent cycloaikyicne radical with three to six carbon atoms, R' is hydrogen, lower alky] or the group -COOH; R° is hydrogen or lower alkyl; n is 0 or an integer from 1 to 13, or R s and R 0 together with the carbon atom to which they attach form a ⁇ C .
- Y is hydrogen, lower alky] or -NRi or -NHaIs; and Z is hydrogen or lower alkyl; and X' is hydrogen, -COOB, -CONH 2 , -SO 3 H, -SO 2 NH 2 Oi -PC-O)(OH) 2 .
- the derivatives include salts.
- N,N-dichloroamino acids of the above formula I in which R ! and R° are not hydrogen, and B/ and R° both are preferably lower alkyl, most preferably methyl.
- R ! and R° are not hydrogen, and B/ and R° both are preferably lower alkyl, most preferably methyl.
- One of the most preferred aganocides is N,N-dichloro-2,2-diracthyi taurine (2"dichloroaniino-2 ⁇ methylpropanesulfoiiate) or its sodium salt.
- Further potentially useful aga.noe.ides are the compounds described in U.S. Patent No.
- a porous material dressing one or more sponges or sterile pieces of foam, tor example, an open-cell polyester or polyether, for example a po!>urethane ester or ether
- a pore size ranging from about.400 to 600 micrometers to maximize tissue growth
- an evacuation tube with various ports that communicate with the foam to control the reduced pressure, to ensure equal distribution of the reduced pressure to all parts of the wound and to prevent wound overgrowth.
- the shape of the sponge or foam may be fashioned to the specific geometry of the wound.
- the foam or sponge may also be preireated with an aganocide described herein, or the aganocide or a precursor of the aganocide may be bound chemically to the foam or sponge, for example, to gradually release aganocide into the wound fluid while it is under negative or under atmospheric pressure.
- the wound is then covered with an adhesive tape to form an airtight system.
- the evacuation tube is connected to a collection device, such as a trap for collecting exudate and any matter debrided from the wound.
- the trap in turn, is connected to an adjustable vacuum pomp, which is able Lo generate the desired negative pressure. It is preferred to equip the collection trap with a nort -return or dart check valve to prevent accidental re-contamination of the wound with exudate.
- the method described herein has the following significant advantages: it accelerates wound closure and/or reduces bacterial count in the wound beyond the rate of wound closure achievable with, the VAC and povidone-iodine, while being completely compatible with wound tissue, and thus being not irritating to the wound as seen with pov ⁇ i one-iodine or silver salts.
- a treatment regimen for promoting wound healing of a mammalian patient having a wound comprising alternating a short-term application with a long-term application to the wound, wherein the short term application comprises contacting the wound with a cell-compatible aganocide composition described herein in an amount effective to benefit wound healing, and wherein the long-terra application comprises contacting the wound with an effective amount of the composition to promote wound healing, and wherein the long-term application is being carried out in lime intervals to promote wound healing and appropriate to the patient in need of wound care, while sufficient negative pressure is applied to the wound for a sufficient time period in conjunction with, or before, during or after application of the aganocide composition, to accelerate the woimd healing process.
- the regimen may be carried out until (i) wound closure is occurring or (il) the size of the wound and/or the bacterial load have been reduced sufficiently to allow self-healing of the wound. While there is no time limit for the regimen, in general a regimen over about 2 ⁇ -- 4 weeks will be sufficient to achieve sufficient wound closure or a satisfactory reduction of the bacterial load of the wound to promote wound healing.
- the regimen is preferred wherein the short term application and the long-term application arc separated by removal of the aganocide composition from the
- the first negative pressure cycle commences after an initial treatment of the wound with the aganoeide composition.
- inorganic or organic aganocide or a source of inorganic or a source of an organic aganocide or a saline composition can be placed again on the wound and any remaining wound debris be removed by gentle atraumatic wiping.
- the vacuum system may also be connected via a valve with a container holding aganocide as described herein. This would allow aganocide treatment between successive negative pressure cycles.
- the treatment regimen although this is less preferred, may begin with a negative pressure step, followed by application of the aganoeidc and repeated alternations of this treatment sequence.
- the combination treatment described herein decreases fluid excess and edema, and thereby bacterial colonization, hi sternal wound infections after cardiac surgery the ICU (Intensive Care Unit) stay of a patient may be significantly shortened by utilizing this combination treatment regimen, ⁇ n addition, the risk of sepsis due to bacteria swept into the blood circulation is markedly decreased.
- the organic aganoeide may also be applied in conjunction with an inorganic aganocide such as HOCl, an HOCI source, or mixtures thereof
- an organic and an inorganic aganocide are applied in ihe method of the invention, preferably, the effective amount of hypoehloroiss acid is from about 20 to about 500 ppm. Even more preferred, the effective amount is from about 40 to about 250 ppm. Most preferably, the effective amount is from about 50 to about 150 ppm.
- the concentration of the two different aganoeides may be appropriately adjusted to meet the requirement of promoting successful wound or ulcer healing, it may be possible that when the initial wound extends over a significant surface area or suffers from significant bacterial infection, the harsher inorganic aganocide, such as HOC], should be applied first until some success in the goals of reducing wound surface area or decreasing bacterial infection is reached. Thereafter, a milder organic aganocide may be applied to complete the wound healing process. However, the actual regimen to be followed is to be determined by the physician or wound healing expert.
- a method for the treatment of a chrome wound using a wound treatment regimen comprising: a) applying reduced pressure to the wound; b) treating the wound with a substantially non-toxic, non- irritating inorganic or organic agaaoeide composition while applying reduce pressure to the A'ound; c) optionally, treating the wound with saline after at least one aganoeide treatment step, wherein steps a), b) and c) together comprising a regimen cycle; and d) optionally, repeating at least one additional treatment regimen cycle.
- the inorganic or organic aganocide composition is selected from the group consisting of hypochlorous acid, a source of hypochlorous acid, an N-halo amino acid, an N,N- dihaloamino acid, a source of an N-halo amino acid, a source of an N,N-diha(oamino acid and mixtures thereof.
- the treatment of the aganocide to the wound is performed before the application of reduced pressure to the wound, in.
- the aganocide treatment of the wound is done after a first application of reduced pressure to the wound,
- the saline treatment is performed in conjunction with applying the reduced pressure.
- the aganocide treatment and the application of reduced pressure is done intermittently.
- each agarsoeidc treatment step is followed by a reduced pressure application step, followed by a second aganocide treatment step, followed by a second reduced pressure application siep, this sequence of steps being considered a single treatment cycle.
- the method comprises a single treatment cycle or at least two single treatment cycles. In another variation, the method comprises at least four, six, ten, twelve or 24 but not more than 48 single treatment cycles.
- the aganocide is an N -halo amino acid or an N,N-dshaioamino acid.
- the treatment with the aganocide is performed by an automated delivery system.
- the N- haio amino acid or an N,N-dihaloamino acid composition has a pH from about 3.5 to 7.
- a method far the treatment of a chronic wound using a wound treatment regimen comprising: a) treating the wound with at least one application of a substantially non-toxic, non-irritating inorganic or organic aganocide composition; b) treating the wound with saline; and c) applying reduced pressure to the wound, in one variation, the inorganic or organic aganocide composition is selected from the group consisting of hypochiorous acid, a source of hypochlorous acid, an N-haio amino acid, an N.N-dihaloammo acid, a source of an N- halo ammo acid, a source of an N,N-dihaloar ⁇ i ⁇ o acid and mixtures thereof.
- the treatment of the aganocide to die wound is performed before the application of reduced pressure to the wound,
- the aganocide tresmieut of the wound is done after a first application of reduced pressure to the ⁇ souud.
- the saline treatment is performed in conjunction w Uh applying the reduced pressure.
- the aganocide treatment and the application of reduced pressure H done intermittently,
- each aga ⁇ oeide treatment step is followed by ⁇ reduced pressure application step, followed b ⁇ a second aganocide treatment step, followed by a second reduced pressure application step, this sequence of steps being considered a single treatment cycle.
- a single treatment cycle or at least two single treatment cycles comprise:? at least four, six. ten. twelve or 24 but not snore than 48 single treatment eyelets.
- the treatment of the wound with saline is applied after at least one uganoeide treatment and one reduced pressure application step.
- a method for promoting wound healing of a mammalian patient having a wound using a treatment regimen comprising: a) a short term application composing contacting the wound with a ceil- compatible inorganic or organic aganocide composition in an amount effective to pronote wound healing; h) a long term application comprising contacting the wound with an cffocthe amount of the aganoeide composition to promote wound healing, wherein the short term application and the long term application are performed at alternating time inter ⁇ als to promote wound healing and appropriate to the patient in need of wound care; and u ) applying reduced pressure to the wound for a sufficient period of time to promote wound healing, in one variation, the aganocide composition is selected from the group consisting of hypochlorous acid, a source of hypochlor ⁇ us acid, an N-halo amino acid, an KN-dihaluamino acid, a source of an N ⁇ halo amino acul a source of an NJN-dihal
- the method comprises one treatment cycle or at least two single treatment cycle?. In another variation, the method comprises at least four, six, ten. twelve or 24 but not more than 48 .single treatment cycles.
- the organic aganoeide is selected from the group convsmig of N-dblorotaurmc, N,N-dich1orotaurine, N, N-diehloro-2,2 ⁇ dimetiiyitaurme. a saJt thereof, and mixtures thereof.
- the treatment with the agair ⁇ culc I? performed by an automated delivery system
- the ⁇ gano ⁇ de composition has a pi?
- die aganocide further includes HOC! or an FIOCl source.
- the amount of aga ⁇ ocide is between about I OO to about 10,000 ppm, about 200 ppm to about 2,000 pp ⁇ i, or about 400 ppm to about 1,000 ppm.
- the amount of HOCl is from about 20 ppm to 500 ppm, about 40 ppm to 250 ppm or about 50 ppm to 150 ppm.
- the pH of the aganocide composition is from about 2 to 9,5, or about 3.5 to 6.5.
- a wound or ulcer treatment system comprising a device adapted to applying reduced pressure to a wound comprising an adhesive cover enclosing the wound or uicer and adapted to maintain reduced pressure around the wound or ulcer and means for supplying reduced pressure to the wound suitable to facilitate healmg of the wound or uicer, means for connecting and disconnecting or shutting oil the reduced pressure from the w ound or ulcer and means for collecting vx ound exudate or removing material from the ⁇ w ound or ulcer: the device being associated with means for suppl> mg a liquid aganoeide composition selected from the group consisting of hypochlorous acid, a hypocblorous and source and mixtures thereof to the wound; and the treatment system being adapted to apphing the aganoeide con ipoM lion before, during or after applying reduced pressure to the wound or ulcer.
- a wound or ulcer treatment system comprising: a dev ice adapted to applying reduced pressure to a wound comprising an adhesne cover enclosing the wound or ulcer and adapted to maintain reduced pressure around the wound or ulcer and means for supplying reduced pressure to the wound suitable to facilitate heaimg of the wound or ulcer, means for connecting, disconnecting, or shutting off the reduced pressure from the wocnd or ulcer and means for collecting tt ⁇ vnd exudate or remov ing material from the wound or ulcer: the device being assc cjaied ⁇ ith means for supph ing a hqiud aganoeide composition selected from the group consisting of an N-halo ammo acid, an N,N-diha!o amino acid, a salt of an N ⁇ halo ammo acid, an N,N-diha!o ammo acid, an N -halo amino acid source, an NJN-dihalo ammo acid
- the liquid aganoeide further includes bj pochlorous acid, a hypochlorous acid source or mixtures thereof, hi another variation of the above, the means for supplying the liquid agano ⁇ de composition are connected to the adhesive cover separately from the means for connecting, disconnecting, or shutting off the reduced pressure from the wound or ulcer and the means for collecting wound exudate or removing material from the wound or ulcer.
- PRf FBRRED EMBODIMEN TS PRf FBRRED EMBODIMEN TS
- the most preferred salt is sodium 2-dich]oroamino-2- ⁇ iiethy]propa ⁇ esulfo ⁇ ate.
- the V.A.C7 Xif!itl! system is perfectly suitable Io deliver the aganocide compositions oftbe present invention in an automated fashion. A complete treatment cycle may work as follows, although other treatment cycles arc also suitable; I. Application of an Inorganic Aganocide and Vacuum Therapy
- the combination therapies as described herein are not limited to the sequence of modes or time ranges shown in the table above.
- the treatment could begin with mechanical treatment of the wound with the inorganic or organic aga ⁇ ocjde, for example, by pouring it on before use of the V. A. C, system.
- the treatment could begin by applying negative pressure first, followed by aganocide treatment etc.
- A human secondary intention wound approximately 3-4 cm in diameter is fitted with a polyuretha ⁇ e sponge, pore size 400-600 microns cut to the size of the wound ami twice the thickness of the wound and covered with an adhesive film, per instructions on the vacuum system.
- the outlet is connected to an IV bag (500 ml) with 0.01% HOCi, 0.45% NaCl and pH of about 4 and is elevated to between 0.1 and 1 meter above the wound.
- a I -cm hole is punctured in the adhesive film, and a second adhesive film is used to cover the hole, with the second outlet connected to the vacuum pump.
- Each cycle consists of three phases: a 2-hour pumping phase when the vacuum ( 125 mm Bg negative pressure) is connected and the I ' V bag is disconnected; a I second instilling phase when the vacuum is disconnected and the IV bag is connected and the aganocide is applied to the wound; and a 15 second holding phase when both vacuum and the IV bag are disconnected.
- Each treatment has about 24 cycles and starts with a pumping phase and proceeds for 48 hours.
- the dressings are changed and the treatment is repeated until full granulation has been promoted and the granulation tissue is within the level of the skin, approximately 30 to 100 days, depending on the depth of the original wound.
- a human secondary intention wound approximately 3-4 cm inches in diameter is fitted with a polyurethane sponge, pore size 400-600 microns cut to the size of the wound and twice the thickness of the woimd and covered with an adhesive film, per instructions on the vacuum system.
- the outlet is connected to an IV bag (500 ml) with 0.01% MOCl, 0.45% NaCl, and pH of about 4 and is elevated to between 0.1 and 1 metes- above the wound.
- a I -cm hole is punctured in the adhesive film, and a second adhesive film is used to cover the hole, with the second outlet connected to the vacuum pump.
- Each cycle consists of three phases; a 2-hour pumping phase when the vacuum (125 ram Ii g negative pressure) is connected and the ⁇ V bag is disconnected; a 5 second instilling phase when the vacuum is disconnected and the ⁇ V bag is connected and the aganocide is applied to the wound; and a 5 minute holding phase when both vacuum and the IV bag are disconnected.
- Each treatment has about 23 cycles and starts with a pumping phase and proceeds for 48 hours.
- the dressings are changed and the treatment is repeated until foil granulation has been promoted and the granulation tissue is within the level of the skin, approximately 30 to iO ⁇ days, depending on the depth of the original wound.
- a human secondary intention wound approximately 3-4 cm in diameter is fitted with a poiyurethane sponge, pore size 400-600 microns cut to the size of (he wound and twice the thickness of the wound and covered with an adhesive film, per instructions on the vacuum system.
- the outlet is connected to an IV hag (500 ml) with, a 0.1% sodium 2 ⁇ dichioroaraino-2-methy]propaR ⁇ suifonate solution adjusted with HC! to a pH of about 4, 0.9% NaCl and elevated io between 0.1 and 1 meter above the wound.
- a 1-cm hole is punctured in the adhesive film, and a second adhesive film is used to cover die hole, with the second outlet connected to the vacuum pump.
- Each cycle consists of three phases: a 2 -hour pumping phase when the vacuum (125 mm Hg negative pressure) is connected and the IV bag is disconnected; a 1 second instilling phase when the vacuum is disconnected and the IV hag is connected and the aganocide is applied io the wound; and a 1 minute holding phase when both vacuum and die IV bag are disconnected.
- Each treatment has about 24 cycles and starts with a pumping phase and proceeds for 48 hours.
- the dressings are changed and the treatment is repeated until full granulation has been promoted and the granulation tissue is within the level of the skin, approximately 30 to 100 days, depending on the depth of the original wound.
- the outlet is connected to as IV bag (500 ml) with a 0.1% sodium 2-dichioroa ⁇ niiio-2-mefhyl ⁇ ropa ⁇ esuifonate solution adjusted with HCI Io a pli of about 4, 0.9% NaCL and is elevated to between 0.1 and i meter above the wound.
- a 1- cm hole is punctured in the adhesive film, and a second adhesive film, is used to cover the hole, with the second outlet connected to the vacuum pump.
- Each cycle consists of three phases: a 2-hour pumping phase when the vacuum ( 125 mm Hg negative pressure) is connected and the IV bag is disconnected; a 5 second instilling phase when the vacuum is disconnected and the IV bag is connected and the aganocide is applied to the wound; and a 30 minute holding phase when both vacuum and the IV hag are disconnected.
- Each treatment has about 19 cycles and starts with a pumping phase and proceeds for 48 hours.
- the dressings are changed and the treatment is repeated until full granulation has been promoted and the granulation tissue is within the level of the skin, approximately 30 to 100 days, depending on the depth of the original wound.
- f ⁇ Ofi ⁇ When compared with treatment of a similar wound with vacuum-assisted wound healing without aganocide or treatment of the wound with aganocide alone the combination treatment regimen described herein achieves superior wound healing results.
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Abstract
Provided herein is a method for the treatment of a wound using a wound treatment regimes for treating a chronic wound, the regimen comprising; a) applying reduced pressure to the wound; and b) treating the wound with a substantially non-toxic, non-irritating organic or inorganic aganocide composition while applying reduced pressure to the wound.
Description
Method for Treatment of Wound Using AgttnocMes
[ΘΘOI 1 I he present invention resales to a method for treating a \\ ours J by applying reduced pressure to the wound, while treating the wound with a substantially non-toxic, non- ^mating organic or inorganic aganoeide composition before, during or after applying reduced pressure to the wound. BAC KGROUND Of THE INVEN TION f (M)OI] The effecth c treatment of open wounds that are too large to sptinianeou-.lv clost hah long been a significant problem Io medical practitioners. Some details explaining these difficulties can be found in U.S. Patent-. 5,636,643 and 5.645.081 , the entire disclosures oi which are incorporated herein by reference. Some of the factors preventing wound closure of permanent open wounds are bacteria! infection of the wound, insufficient blood circulation άϊiά the presence of necrotized tissue. Louis C. Argeπta and Michael J. Morykw as introduced the \acuum-assisted wound closure (YAC) system m 1^7 for the treatment υi" pressure ulcers and other chronic wounds (Ann Plast Surg IW : ^8:553-562 and ς63-57"). This system is based on the application of negative pressure to the wound, resulting in arteriolar dilatation and promoting granulation tissue prokferatmn and resulting in substantially impro\ ed wound healing conditions. {9003 J As concerns bacteπal sniecuon of an open wound, a bacterid count of 1 ty or more per grain of tissue is defined us infected. Ii H known that wound (issue ihat is infected \\ ύ not be si^cepfeble io wound closure. Therefore, a number of approaches have been taken to reduce bacterial infection, for example, iodine or silver salts have beer? used to eliminate bacterial infection thai would prevent wound closure However, man> patients are sensitive or allergic to iodine treatment of wounds, and even more so to si her salts, hi addition, siher salts ha\c not been very effecthe, and have reported to cause argyria, a permanent stainmy of the skm. In order to mitigate ihese effects, ptn suone iodine ha_» been used, but some patient sensuivifv or allergic reactions will reπia.n, Dakm's solution (a buffered solution of NaOC 1 of a mild basic pH) i:> also used Itεi|ueτu]y for the treatment of open woundt> but is nor verv effective, !n addition, DaLhYs soh:tion Ls cytotoxic, which impedes wound healing. Therefore, wound healing experts discourage the use of Dakin's solution, (t has been also proposed to apply antibiotics topical)) , but the resistance to treatment with antibiotics are a standing and lingering concern.
{00041 More recently, organic or inorganic aganocides have been proposed such as
HOCI and N-halo or N,N-dihalotaurme derivatives to substantially reduce bacteria! count in the treatment of open wounds (U.S. Patent No. 6,426,066 and U.S. Publication No.
2004/0!37078 AI). fOOOS] Taijana M. fleck et ai. have proposed applying negative pressure for the treatment of deep sternal wound infections after cardiac surgery and have described debridement with removal of all necrotic tissue and irrigation with dilute povidone-iodine solution and I bO? before and after application of the negative pressure (TM. Fleck et ai in Ann Thorac Surg 2002; 74; 1596-200). However, as explained before, certain patients are allergic and sensitive to povidone-iodine treatment.
SUMMARY OF THE INVENTION
HMMMl Ii is understood that any aspect or feature of the present invention whether characterized as preferred or not characterized as preferred may be combined with any other aspect or feature of the invention, whether such other feature is characterized as preferred or not characterized as preferred. For example, a feature described as preferred. for example a pH range, or a specific pH for a particular" composition may be combined with another not preferred feature, for example, a NaCl concentration of 1 ,2%, without deviating from the present invention.
DEFINFTIONS
|GOΘ7) "Aganocide" as used herein, refers to N-halo or N.N-dihalo amino acids, or
N-halo- or N,N-dihaio amino acid sources. See "N-HaIo or N,N~Dihalo Amino Acid
Source" beiow. fOOβSf "Atraumatic wiping" refers to removal of undesirable matter from or around the wound or skin around the wound without causing trauma cither to newly formed tissue or the skin around the wound.
[Θ009J "Bacterial load" refers to the bacterial count in a wound determined by wound biopsy.
"Biopsy" refers Io a method of assessing the infection of a wound or ulcer. I ! J ''Cell-compatible composition" is an aganocide composition or a NaC! containing composition with acceptable cytotoxicity and is physiologically acceptable for therapeutic applications.
[OSI 2] "Combination treatment regimen" is a treatment regimen applying vacuum- assisted wound treatment with use of an aganocide as described herein.
^0013] "Debridement" refers to the removal of tissue ia and around the wound that may negatively affect wound healing or closure.
|ΘOi4| "Exudate" refers Io a liquid oo/ing out from a wound that may affect wound healing in a positive or negative sense, jOθiSj "Granulation" refers to a secondary indention wound as defined below thai is vascularizing and healing from the bottom up. Granular appearance (bumpinesss of the wound is not necessary for a wound to be classified as granular.
[Oθt&j "K-HaIo or N.N-dihalo ammo acid source" refers to a composition that releases an N-haio- or N,N-dihalo amino acid for example, a salt, such as the sodium, potassium, magnesium or calcium salt, that, when treated with an acid releases the conespondmg TN-halo- or N,N-Dihalo Λnu'no Acid. As an alternative for example, a
N JN-dihak> amino acid source may be a polymeric matrix that is stable toward the N-HaIo or NΛ'- diaalo amino acid (or aganocide) but gradually releases the N-HaIo or NLN-dihalo amino acid into the wound,
[00171 "Hvpøchlorous acid source" or "HOCl source" refers to a composition that releases, or under certain conditions, is capable of releasing OC)Ci For example, NaOCI or KOCl that is treated with an acid releases HOCi.
JOIIl 8] "Long-term application" is differentiated from "Short-term application" and refers to the extended application of a component of the regimen to the wound or ulcer.
As i, rule it wiii be an application for more than 120 minutes, preferably for more than 6 hours.
|ΘO1<ϊJ "Precede" refers to a step, procedure or application in the regimens described herein that goes before a step, procedure or application that follows.
IQQlM] "'Regimen" is a systematic topical therapy plan or schedule designed to promote the healing of wounds or ulcers.
JΘΘ21] "Removal of a composition"' refers to the separation of a matter or a composition from the wound, ulcer or skin around the wound, for example, of a component of the regime described.
[0022] "Saline" is a NaCI containing composition, mostly in form of a solution that us physiological 1\ acceptable to the mammalian skin in the area of the wound or ulcer; it may also contain other alkali or earth-alkali ions.
|0δ23| "Secondary intention wound" is a wound in which some tissue has been lost and healing is predominantly done by cleaning and allowing self-regeneration.
|8Θ24] "Short-term application" is differentiated from "long-term application" and refers to the temporary application of a component of the regimen to the wound or ulcer.
As a rule it will be an application for not more than 120 minutes. j 0925| "The size of the wound and/or the bacterial load have been reduced sufficiently" refers to the wound healing progress achieved after application of the regimen herein described to such an extent that the wound-healing would continue even if the regimen would be discontinued.
[0026] "Time intervals appropriate to patients in need of wound care" refer to the io.ng-tcr.rn application of a component or composition of the regimen beneficial in terms of promoting wound healing, enhancing patient compliance and in a health care facility coincide with patient visits by physicians, nurse practitioners, nurse assistants and personnel capable of performing the wound care required by the various regimens described herein.
[0027J "Time kill" refers to an in vitro measure of how fast a given antimicrobial can kill test bacteria.
10028"! "'Treatment cycle" refers to the completion of a regimen after a number of repetitions of the alternating steps, as defined herein.
{0029] "Wiping" is a specific removal process by nibbing or cleaning or drying, in general without causing infection of a wound or ulcer.
[0030| "Wound or ulcer"' is a break in external skin accompanied by loss of skin surface tissue, disintegration and necrosis of epithelial tissue,
[00311 "Wound closure is occurring" refers to the wound healing progress achieved after application of the regimen herein described. In general, such progress is achieved when the fraction of open wounds is reduced by at least 70 % relative to the open woands existing prior to administration of the regimens described herein.
[0632] "Wound dressing" refers to a protective or therapeutic material applied to a wound minimizing infection and producing an environment beneficial to promoting wound healing.
[0033] "Wound tracing" refers to various methods or a specific method to measure and / or evaluate wound surface area, jθ034| In accordance with the present invention we provide a new method for treating a wound, in particular a chronic wound, by applying reduced pressure to the wound, while treating the wound with a substantially non-toxic, non-irritating member of
an inorganic sganoeide selected from the group consisting of hypochlorous acid, an inorganic source of hs pochlorous acid and mixtures thereof, bypochiorous acid, an ϊnoignnic source of hypochlorous acid and mixtures thereof, in conjunction with, or before, during or after applying reduced pressure to the wound. Alternatively, an orgaπic agaiυαde composition selected from the group consisting of an N-HaIo amino acid, an VN-ώhalo ammo acid, a source of N-HaIo amino acid, a source of N.N-chtialo ammo acic, and mixtures thereof, may also be used m conjunction with, or before, dunng or after apphmg reduced pressure to the wound,
[0035] The method may use an> de\ ice or combination of devices suitable for delivering the organic or the inorganic aganocidc composition^ intermittently to the wound and for aρpl> mg negative pressure (i.e., vacuum) io the wound. Deuces include the one described m US 5,63C\643, 5,645,08! or US 7,216,651 or a de\iee as described in \nκ ϊliorac Surg 2002; 74:15^6-1600, (VAC s>slera provided b> KCI Inc., San Antonio, Texas), or m KCFs Y A.C. r '"""'' Brochure and the Versatile 1 ' M Wound V acuuni System as described on the website of BlueSky Medical {.currently http:>'/v. \vλ .bkieskyuiedK'si.coTU/).
|ΘΘ3&| However, the inorganic or organic aganocidc composition may also be delh ered to ihe wound before, or after applying the vacuum de\ see or system, or between iwu oi more applications of ihe vaeunrn system to the wound. In this embodiment, the inorganic or organic agaαodde composition may be sprayed or poured on the wound or applied by injection, by irrigation, by dripping ύ on the wound, or gentle rubbing it onto ihe wound, or touching the wound, or by any form of contact that achiexes its" application onto the wound without negatively affecting \u)und healing. In one particular aspect, it is understood that m certain applications, the vacuum system may not he used for agaruvide delivery, but only for application of the vacuum.
^0037J The new treatment method is effective to promote healing of difficult healing v.ouuds. The aganocide composition and reduced pressure are applied to a wound for a sufficient time mid with sufHαem concentration of die aganocide and sufficiently reduced pressure to facilitate wound closure. This can be achieved by many different approaches as described sn more detail below, DFTAILED DESCRIPTION
|0838f jVUxst frequently m the treatment regimen described herei , before applying negative pressure, necrotic tissue is remov ed and the organic or inorganic aganocide
composition applied to the wound. Sometimes, in case of severe v> ouncis. before application of the aganocidc composition, the wound ma}' bε traced to determine its surface area. Biopss may also be used to ascertain the degree of infection of tbe wound. The aganocide composition may be applied by spraying it cm the wound, b> injection, by pouring it on the wound, by irrigation, by using a soft carrier containing the aganoctde and contact^ or touching the wound lightly with the carrier containing the aganocide. Thereafter, a cover, such as an impermeable cover is fitted on the wound to cover and eiidose the wound. As described in more detail below, a suitable porous material ma> be placed into or over the wound before the impermeable co\er is fitted on ihe wound. Tbe mip.Tineable cover is configured to maintain reduced pressure at the site of the wound. The eo\ er is connected with a vacuum pump to apply a vacuum or reduce pressure to the %\υurκi. The cover is then sealed, for example, v* ith drape to provide an airtight seal. Thereafter suction is applied to collect wound fluid that is removed b> suction. [0039) The inorganic aganocidc composition should be applied in concentration of up t«j ! O1OOO pprn. Preferably, the effective amount of hypochlorous acid is from about 20 to about 500 ppm. Even more preferred, the effective amourn is from about 40 to about 250 ppni. Most preferably, the effective amount is from about 50 to about 150 ppir.
[0040] If the aganocide is.sed is an organic aganocidc composition such as an N- halogenatcd or \',N-dihalogemned amino acid or a derivative thereof, for example, as described m WO02221 18 W. Gmtardi, U.S. Publication No. 2005 00651 15 A I and U.S. Serial No 1 1 '339,9S"7 filed January 25. 2006, the aganoπde concentration to be chosen shot Id be higher than the inorganic aganocides because the organic agauociάes have a mile antibacterial action. The mono-lialogcnaied ammo acids (i.e. N-halo amino acids), such as N-ehlorotamine. have the mildest antibacterial action among all N-halogenateri amino acids, milder than the antibacterial action of N,N-dichIorotaυrine or N,N-diehloro- 2,2-ditncthyl taurine. Therefore the higher concentration ranges may be used for the N- irjonohnlogeuated amino acids. Preferably, the effective amount of an organic aganocide such as an N- halogenated amino acid is from about 100 to about 10,000 ppm. Even more preferred, ihe effective amount is from about 200 to about 2,QGO ppm. Most preferably, the effective amount it> from about 400 to about J ,000 ppm. [004 ϊ j The pH of the inorganic aganocide compositions which are prefer&biy applied in form of aqueous solutions is not critical as long it is acidic io neutral A broad
pi I range from about 2 to about 7 may be a useful range. A more preferred pH range would be between about 3 and about 7, and a pH range between about 3,5 to about 7 may be most preferred. If an acidic pH range is the best choice for a given wound condition, a pH between about 3,5 to about 7 is preferred. Evzn more preferred is a pH in a range of between about 3.5 and about. 6.5.
JΘΘ42] The pH of the organic agaπocide compositions which are preferably applied in form of aqueous solutions is not critical although a broad pH range from about 2 to about 9.5 may be a useful range. A more preferred pH range would bε between about 3 and about 9, and a pH range between about 3.5 to about 8.5 may be most preferred. If an acidic pf i range is the best choice for a given wound condition, a pH between about 3.5 to about 7.5 is preferred. Even more preferred may be a range between about 3,5 and about 6.5,
|0843| If the inorganic or organic aganocide composition is used in the form of a solution, lhen water or a lower alcohol (such as ethanol or isoproparsol) or mixtures thereof may be the best choice for the vehicle. If an aqueous composition is being used it would be most advantageous to use a saline composition (for example, NaCl) in amounts of about 0.4 to about 1.2 % by weight of NaCi, or the equivalent of a similar salt such as KCK because such compositions would be even less irritating. (Θ044) The method described herein includes at least one step of applying the inorganic or organic aganocide to the wound and its removal or replacement, for example, before application of the vacuum. In one particular embodiment, the aganocide may also be applied while the vacuum is on by a device having a separate port for delivering the aganocide composition with a shut-off valve which would initially be in a closed position while the wound is under reduced pressure. Then a valve controlling the port to the vacuum pump would be shut off and the valve controlling delivery of the aganocide composition would be slowly and carefully opened to apply a certain often predetermined amount of aganocide to the wound. After shutting off the aganocide delivery valve, the vacuum can be re-applied, resulting in removal of wound exudate and excess aganocide.
|0D45| A preferred embodiment comprises at least two applications of (he inorganic or organic aganocide, or one application of aganocide followed by saline treatment and at leas! one application of reduced pressure, to debride the wound and reduce bacterial court, for example, before the application of the vacuum, and after the vacuum treatment
has been discontinued. This method would have the benefit that bacterial COUTH of the wound is reduced before negative pressure (vacuum) is applied that will result in an even further reduced bacterial count. After the vacuum has been shut off, further debridement with the aganocide or with saline may be performed.
[0046| Au even more preferred treatment involves an intermittent treatment regimen, for example as follows:
1. Deliver the agarsαcidε composition to the wound under sterile conditions a) by spraying; b) by injection; c) by pouring the composition on the wound; and/or d) by irrigation;
2. Leave composition on the wound for i second to 120 minutes or 5 minutes to 60 minutes;
3. Remove the composition by gentle atraumatic wiping or reduced pressure (vacuum);
4. Apply reduced pressure for more extended period (for example, I minute ■■ 12 hrs);
5. Shutting off the vacuum;
6. Reapply the composition (aganocide or saline); and optionally
7. Repeat the treatment cycle
|0047) A complete treatment cycle comprises at least two applications of the inorganic or organic aganocide composition and lasts at least 24 hours during which negative pressure is applied.
JΘ048J Alternatively, prior to applying negative pressure the aganocide composition applied to the wound may be removed by changing the dressing, changing the dressing and wiping, delivery of fresh aganocide composition and rinsing off aganocide residues, such as by using water and/or saline solution, from previously applied aganocide or pouring on new aganocide composition and rinsing off aganocide residues from previously applied aganocide.
|0049| A typical treatment regimen will last from at least 1.2 hours and can last up to several weeks and will comprise multiple treatment cycles until the status of sufficient wound closure has been achieved to enable self-healing of the wound. It is preferred to achieve wound closure or the status of self-healing in two weeks or less time.
[øϋSOf The reduced pressure to be applied may range from about 0.01 to about 0.99 atmospheres, more preferably from about 0.03 to about 0.8, or from about 0.03 to about 0,4 atmospheres, A preferred reduced pressure applies at least 0.1 1 or 0.136 aim to the wound. In certain applications, it is beneficial to provide the reduced pressure in an intermittent or cyclic manner which may be achieved by manual or automatic control of the vacuum system. Details of the VAC treatment regimen are described in U.S. Patent No. 5,636,643 and U.S. Patent No. 7,216,651,
[ 00511 Preferred organs c aganoci des arc N-chlorotaurine or N,N -dichl orotauri nc and their salts, and N-lialo- orN,N~dihaloamino acids of the formula (I)
A-OR1FOR(CH2VC(YZ)-X1 (I)
\i}(l52\ or a derivative thereof. In this formula A is hydrogen, HaINIi- or RahN- wherein Hal is halogen selected from the group consisting of cliloro and bromo; R is a carbon carbon single bond or a divalent cycloaikyicne radical with three to six carbon atoms, R' is hydrogen, lower alky] or the group -COOH; R° is hydrogen or lower alkyl; n is 0 or an integer from 1 to 13, or Rs and R0 together with the carbon atom to which they attach form a {C.rQ)cycloalkyl ring; Y is hydrogen, lower alky] or -NRi or -NHaIs; and Z is hydrogen or lower alkyl; and X' is hydrogen, -COOB, -CONH2, -SO3H, -SO2NH2 Oi -PC-O)(OH)2. The derivatives include salts.
|0053 i Preferred arc the N,N-dichloroamino acids of the above formula I in which R! and R° are not hydrogen, and B/ and R° both are preferably lower alkyl, most preferably methyl. One of the most preferred aganocides is N,N-dichloro-2,2-diracthyi taurine (2"dichloroaniino-2~methylpropanesulfoiiate) or its sodium salt. Further potentially useful aga.noe.ides are the compounds described in U.S. Patent No. 4,386,103 to Pogaoy, the disclosure of which is incorporated herein by reference in its entirety, JΘ054] In order to apply the VAC system to a wound pretreated with an organic agaπocide composition, generally first a porous material dressing (one or more sponges or sterile pieces of foam, tor example, an open-cell polyester or polyether, for example a po!>urethane ester or ether) with a pore size ranging from about.400 to 600 micrometers to maximize tissue growth is placed on the wound. Embedded in the foam or sponge is an evacuation tube with various ports that communicate with the foam to control the reduced pressure, to ensure equal distribution of the reduced pressure to all parts of the wound and to prevent wound overgrowth. The shape of the sponge or foam may be fashioned to the specific geometry of the wound. The foam or sponge may also be
preireated with an aganocide described herein, or the aganocide or a precursor of the aganocide may be bound chemically to the foam or sponge, for example, to gradually release aganocide into the wound fluid while it is under negative or under atmospheric pressure.
{0055] The wound is then covered with an adhesive tape to form an airtight system. The evacuation tube is connected to a collection device, such as a trap for collecting exudate and any matter debrided from the wound. The trap, in turn, is connected to an adjustable vacuum pomp, which is able Lo generate the desired negative pressure. It is preferred to equip the collection trap with a nort -return or dart check valve to prevent accidental re-contamination of the wound with exudate.
|δ056| The method described herein has the following significant advantages: it accelerates wound closure and/or reduces bacterial count in the wound beyond the rate of wound closure achievable with, the VAC and povidone-iodine, while being completely compatible with wound tissue, and thus being not irritating to the wound as seen with povκi one-iodine or silver salts.
JJΘGS7] More specifically, there is provided a treatment regimen for promoting wound healing of a mammalian patient having a wound, wherein the regimen comprises alternating a short-term application with a long-term application to the wound, wherein the short term application comprises contacting the wound with a cell-compatible aganocide composition described herein in an amount effective to benefit wound healing, and wherein the long-terra application comprises contacting the wound with an effective amount of the composition to promote wound healing, and wherein the long-term application is being carried out in lime intervals to promote wound healing and appropriate to the patient in need of wound care, while sufficient negative pressure is applied to the wound for a sufficient time period in conjunction with, or before, during or after application of the aganocide composition, to accelerate the woimd healing process. {ΘΘS8J In general, the regimen may be carried out until (i) wound closure is occurring or (il) the size of the wound and/or the bacterial load have been reduced sufficiently to allow self-healing of the wound. While there is no time limit for the regimen, in general a regimen over about 2 ■-- 4 weeks will be sufficient to achieve sufficient wound closure or a satisfactory reduction of the bacterial load of the wound to promote wound healing. The regimen is preferred wherein the short term application and the long-term application arc separated by removal of the aganocide composition from the
IO
wound. However, over the entire cycle of alternations or the wound-treating cycle it is immaterial whether fee cycle started with a short-term or a long-term application of the agarsocidc, although a treatment regimen starting with a short-term application of the aganocide will be preferred, because it is likely to produce more quickly beneficial results. Good results are achievable if the first negative pressure cycle commences after an initial treatment of the wound with the aganoeide composition. In one preferred procedure, after each negative pressure cycle, inorganic or organic aganocide or a source of inorganic or a source of an organic aganocide or a saline composition can be placed again on the wound and any remaining wound debris be removed by gentle atraumatic wiping. The vacuum system may also be connected via a valve with a container holding aganocide as described herein. This would allow aganocide treatment between successive negative pressure cycles. Alternatively, the treatment regimen, although this is less preferred, may begin with a negative pressure step, followed by application of the aganoeidc and repeated alternations of this treatment sequence. [0059) The combination treatment described herein decreases fluid excess and edema, and thereby bacterial colonization, hi sternal wound infections after cardiac surgery the ICU (Intensive Care Unit) stay of a patient may be significantly shortened by utilizing this combination treatment regimen, ϊn addition, the risk of sepsis due to bacteria swept into the blood circulation is markedly decreased.
(θθ&©| The organic aganoeide may also be applied in conjunction with an inorganic aganocide such as HOCl, an HOCI source, or mixtures thereof If an organic and an inorganic aganocide are applied in ihe method of the invention, preferably, the effective amount of hypoehloroiss acid is from about 20 to about 500 ppm. Even more preferred, the effective amount is from about 40 to about 250 ppm. Most preferably, the effective amount is from about 50 to about 150 ppm. However, as necessary, the concentration of the two different aganoeides may be appropriately adjusted to meet the requirement of promoting successful wound or ulcer healing, it may be possible that when the initial wound extends over a significant surface area or suffers from significant bacterial infection, the harsher inorganic aganocide, such as HOC], should be applied first until some success in the goals of reducing wound surface area or decreasing bacterial infection is reached. Thereafter, a milder organic aganocide may be applied to complete the wound healing process. However, the actual regimen to be followed is to be determined by the physician or wound healing expert.
EMBODrMENTS AND ASPECTS OF TIiE INVENTION
In one embodiment of the application, there is provided a method for the treatment of a chrome wound using a wound treatment regimen, the regimen comprising: a) applying reduced pressure to the wound; b) treating the wound with a substantially non-toxic, non- irritating inorganic or organic agaaoeide composition while applying reduce pressure to the A'ound; c) optionally, treating the wound with saline after at least one aganoeide treatment step, wherein steps a), b) and c) together comprising a regimen cycle; and d) optionally, repeating at least one additional treatment regimen cycle. In one variation, the inorganic or organic aganocide composition is selected from the group consisting of hypochlorous acid, a source of hypochlorous acid, an N-halo amino acid, an N,N- dihaloamino acid, a source of an N-halo amino acid, a source of an N,N-diha(oamino acid and mixtures thereof. In another variation, the treatment of the aganocide to the wound is performed before the application of reduced pressure to the wound, in. another variation, the aganocide treatment of the wound is done after a first application of reduced pressure to the wound, In another variation, the saline treatment is performed in conjunction with applying the reduced pressure. In another variation, the aganocide treatment and the application of reduced pressure is done intermittently. In another variation, each agarsoeidc treatment step is followed by a reduced pressure application step, followed by a second aganocide treatment step, followed by a second reduced pressure application siep, this sequence of steps being considered a single treatment cycle. In yet another variation, the method comprises a single treatment cycle or at least two single treatment cycles. In another variation, the method comprises at least four, six, ten, twelve or 24 but not more than 48 single treatment cycles. In another variation, the aganocide is an N -halo amino acid or an N,N-dshaioamino acid. In another variation, the treatment with the aganocide is performed by an automated delivery system. In another variation, the N- haio amino acid or an N,N-dihaloamino acid composition has a pH from about 3.5 to 7. In another embodiment, there is provided a method far the treatment of a chronic wound using a wound treatment regimen, the regimen comprising: a) treating the wound with at least one application of a substantially non-toxic, non-irritating inorganic or organic aganocide composition; b) treating the wound with saline; and c) applying reduced pressure to the wound, in one variation, the inorganic or organic aganocide composition is selected from the group consisting of hypochiorous acid, a source of hypochlorous acid, an N-haio amino acid, an N.N-dihaloammo acid, a source of an N-
halo ammo acid, a source of an N,N-dihaloarπiπo acid and mixtures thereof. In another variation, the treatment of the aganocide to die wound is performed before the application of reduced pressure to the wound, In another variation, the aganocide tresmieut of the wound is done after a first application of reduced pressure to the \souud. In another variation, the saline treatment is performed in conjunction w Uh applying the reduced pressure. In another variation, the aganocide treatment and the application of reduced pressure H done intermittently, In another variation, each agaπoeide treatment step is followed by α reduced pressure application step, followed b\ a second aganocide treatment step, followed by a second reduced pressure application step, this sequence of steps being considered a single treatment cycle. In another variation of the method, a single treatment cycle or at least two single treatment cycles, In another variation, the method comprise:? at least four, six. ten. twelve or 24 but not snore than 48 single treatment eyelets. In another variation, the treatment of the wound with saline is applied after at least one uganoeide treatment and one reduced pressure application step.
In another embodiment, there is provided a method for promoting wound healing of a mammalian patient having a wound using a treatment regimen, wherein the regimen comprises: a) a short term application composing contacting the wound with a ceil- compatible inorganic or organic aganocide composition in an amount effective to pronote wound healing; h) a long term application comprising contacting the wound with an cffocthe amount of the aganoeide composition to promote wound healing, wherein the short term application and the long term application are performed at alternating time inter\ als to promote wound healing and appropriate to the patient in need of wound care; and u ) applying reduced pressure to the wound for a sufficient period of time to promote wound healing, in one variation, the aganocide composition is selected from the group consisting of hypochlorous acid, a source of hypochlorυus acid, an N-halo amino acid, an KN-dihaluamino acid, a source of an N~halo amino acul a source of an NJN-dihalαamino acid, and a compound of the formula
A-C(R* 1OR(CH, J11-C(YZVX' (I) or a derivative thereof, or mixtures thereof, wherein A is hydrogen, IlalKH- or HaI^N- wherein HaI is halogen selected from the group consisting of chloro and broπrø; R is a carbon single bond or a divalent cyeioalkyleπe radical with three to six carbon atoms, R1 is hydrogen, lower alkyl or the group -COOH; R° is hydrogen or lower alky!; n is O or an
integer from I to 13. or R ! and R ' together with the carbon aiom to which they attach form a (tV(Λ)eydoaikyl ring; Y is hydrogen, lower alky! or -KIh m -NHah; and Z is hydrogen or low cr alkyl: and X' is hydrogen. COOH. -CONIl;, -SCM!, -Si KNH2 or -P(O)(QH h, a salt thereof, a derivative thereof, and mixtures thereof. In another variation, the short term and or long term application of the agaπocide to the wound is performed bct'orc the application of reduced pressure to ihe wound. In another variation, the short term and or long ierm application of the aganocide to the wound ib done after a first appϊ seat ion of i educed pressure to the wound in another variation, the saline treatment is pes formed m conjunction \\)th applying the reduced pressure, In another variation, tiie short term and/or the long term application of the aganocidε and the application of reduced pressure is done intermittently. In another \ariation, each of the iihoit tenn and/or each of the long term aganocide application step is followed by a reduced pressure application step, followed b> a second short term and'or long term agaiioeide application step, followed by a second reduced pressure application step, this sequence? of .steps being considered a single treatment cycle. In another variation, the method comprises one treatment cycle or at least two single treatment cycle?. In another variation, the method comprises at least four, six, ten. twelve or 24 but not more than 48 .single treatment cycles. In another variation, the organic aganoeide is selected from the group convsmig of N-dblorotaurmc, N,N-dich1orotaurine, N, N-diehloro-2,2~ dimetiiyitaurme. a saJt thereof, and mixtures thereof. In another \ anation, the treatment with the agairøculc I?: performed by an automated delivery system, in another variation, the εganoαde composition has a pi? from abosu 3.5 to 7 In another variation, die aganocide further includes HOC! or an FIOCl source. In another variation, the amount of agaπocide is between about I OO to about 10,000 ppm, about 200 ppm to about 2,000 ppπi, or about 400 ppm to about 1,000 ppm. In another variation, the amount of HOCl is from about 20 ppm to 500 ppm, about 40 ppm to 250 ppm or about 50 ppm to 150 ppm. In anothei variation, the pH of the aganocide composition is from about 2 to 9,5, or about 3.5 to 6.5.
In another embodiment, there is provided a wound or ulcer treatment system comprising a device adapted to applying reduced pressure to a wound comprising an adhesive cover enclosing the wound or uicer and adapted to maintain reduced pressure around the wound or ulcer and means for supplying reduced pressure to the wound suitable to facilitate healmg of the wound or uicer, means for connecting and
disconnecting or shutting oil the reduced pressure from the w ound or ulcer and means for collecting vx ound exudate or removing material from the ■w ound or ulcer: the device being associated with means for suppl> mg a liquid aganoeide composition selected from the group consisting of hypochlorous acid, a hypocblorous and source and mixtures thereof to the wound; and the treatment system being adapted to apphing the aganoeide con ipoM lion before, during or after applying reduced pressure to the wound or ulcer.
In yet another embodiment, there ?s provided a wound or ulcer treatment system comprising: a dev ice adapted to applying reduced pressure to a wound comprising an adhesne cover enclosing the wound or ulcer and adapted to maintain reduced pressure around the wound or ulcer and means for supplying reduced pressure to the wound suitable to facilitate heaimg of the wound or ulcer, means for connecting, disconnecting, or shutting off the reduced pressure from the wocnd or ulcer and means for collecting tt ϋvnd exudate or remov ing material from the wound or ulcer: the device being assc cjaied \\ ith means for supph ing a hqiud aganoeide composition selected from the group consisting of an N-halo ammo acid, an N,N-diha!o amino acid, a salt of an N~halo ammo acid, an N,N-diha!o ammo acid, an N -halo amino acid source, an NJN-dihalo ammo acid source, a salt thereof, and mixtures thereof to the wound; and the treatment svhtern being adapted to applying the agaπocide composition before, during or after applying reduced pressure to the wound or ulcer. In one variation, the liquid aganoeide further includes bj pochlorous acid, a hypochlorous acid source or mixtures thereof, hi another variation of the above, the means for supplying the liquid aganoαde composition are connected to the adhesive cover separately from the means for connecting, disconnecting, or shutting off the reduced pressure from the wound or ulcer and the means for collecting wound exudate or removing material from the wound or ulcer. PRf FBRRED EMBODIMEN TS
(00(^1 J tn a particular aspect, it is. preferred to automate the deliver} of a topical solution of the inorganic or organic aganoeide composition described herein and its removal from the w ound because it assists with wound cleansing, irrigation and remov al of is fcctiOLis materials and minimises costly and time -consuming caregiver intervention. Λs t'escπbed abo\ e, if the agaπocidc composition of the invention is administered during the application of the VAC system, and if its administration and removal is automated. the VAC* system can be used in a homecare setting and human caregiver's time inv olvement may be limited to teaching and training the patient to use such an automated
VAC system. Details of this combination therapy are described in KCFs V.A.C.^"*11'1 brochure, the entire text of which is incorporated herein by reference {frhlp://www.kci! xom/29-A- l42instiliBrochurci2-05.pdf). |ΘO62| if an N-halo- or N,N-dihalo amino acid is used as the aganocide, at the preferred pi I range described herein, the aganocide is present in a salt form, preferably in an inorganic salt form, such as the sodium, potassium, magnesium, calcium, aluminum, etc. salt. The most preferred salt is sodium 2-dich]oroamino-2-τiiethy]propaπesulfoπate. |OΘ63j The V.A.C7Xif!itl!! system is perfectly suitable Io deliver the aganocide compositions oftbe present invention in an automated fashion. A complete treatment cycle may work as follows, although other treatment cycles arc also suitable; I. Application of an Inorganic Aganocide and Vacuum Therapy
Application of an Organic Aganocide and Vacuum Therapy
However, the combination therapies as described herein are not limited to the sequence of modes or time ranges shown in the table above. For example, the treatment could begin with mechanical treatment of the wound with the inorganic or organic agaπocjde, for example, by pouring it on before use of the V. A. C, system. Alternatively, the treatment could begin by applying negative pressure first, followed by aganocide treatment etc.
Example 1 (Hypochϊorous Acid) ff)0s$5| A human secondary intention wound approximately 3-4 cm in diameter is fitted with a polyurethaπe sponge, pore size 400-600 microns cut to the size of the wound ami twice the thickness of the wound and covered with an adhesive film, per instructions on the vacuum system. The outlet is connected to an IV bag (500 ml) with 0.01% HOCi, 0.45% NaCl and pH of about 4 and is elevated to between 0.1 and 1 meter above the wound. A I -cm hole is punctured in the adhesive film, and a second adhesive film is used to cover the hole, with the second outlet connected to the vacuum pump. [8066] Each cycle consists of three phases: a 2-hour pumping phase when the vacuum ( 125 mm Bg negative pressure) is connected and the I'V bag is disconnected; a I second instilling phase when the vacuum is disconnected and the IV bag is connected and the aganocide is applied to the wound; and a 15 second holding phase when both vacuum and the IV bag are disconnected.
[0067| Each treatment has about 24 cycles and starts with a pumping phase and proceeds for 48 hours. When the treatment is completed, the dressings are changed and the treatment is repeated until full granulation has been promoted and the granulation tissue is within the level of the skin, approximately 30 to 100 days, depending on the depth of the original wound.
|0t>68| When compared with treatment of a similar wound with vacuum-assisted wouBd healing without aganocide or treatment of the wound with aganocide alone the combination treatment regimen described herein achieves superior wound healing results.
Example 2 (ϊ-iypochlorous Acid)
(0069) A human secondary intention wound approximately 3-4 cm inches in diameter is fitted with a polyurethane sponge, pore size 400-600 microns cut to the size of the wound and twice the thickness of the woimd and covered with an adhesive film, per instructions on the vacuum system. The outlet is connected to an IV bag (500 ml) with 0.01% MOCl, 0.45% NaCl, and pH of about 4 and is elevated to between 0.1 and 1 metes- above the wound. A I -cm hole is punctured in the adhesive film, and a second adhesive film is used to cover the hole, with the second outlet connected to the vacuum pump. [0070] Each cycle consists of three phases; a 2-hour pumping phase when the vacuum (125 ram Ii g negative pressure) is connected and the ΪV bag is disconnected; a 5 second instilling phase when the vacuum is disconnected and the ΪV bag is connected and
the aganocide is applied to the wound; and a 5 minute holding phase when both vacuum and the IV bag are disconnected.
[0071 ] Each treatment has about 23 cycles and starts with a pumping phase and proceeds for 48 hours. When the treatment is completed, the dressings are changed and the treatment is repeated until foil granulation has been promoted and the granulation tissue is within the level of the skin, approximately 30 to iOϋ days, depending on the depth of the original wound.
When compared with treatment of a similar wound with vacuum-assisted wound healing without aganocide or treatment of the wound with aganocide alone the combination treatment regimen described herein achieves superior wound healing results.
Example 3 {Sodium 2-dichloroamino-2-niethy!propanesu]fonate)
|Θ873J A human secondary intention wound approximately 3-4 cm in diameter is fitted with a poiyurethane sponge, pore size 400-600 microns cut to the size of (he wound and twice the thickness of the wound and covered with an adhesive film, per instructions on the vacuum system. The outlet is connected to an IV hag (500 ml) with, a 0.1% sodium 2~dichioroaraino-2-methy]propaRεsuifonate solution adjusted with HC! to a pH of about 4, 0.9% NaCl and elevated io between 0.1 and 1 meter above the wound. A 1-cm hole is punctured in the adhesive film, and a second adhesive film is used to cover die hole, with the second outlet connected to the vacuum pump. [00741 Each cycle consists of three phases: a 2 -hour pumping phase when the vacuum (125 mm Hg negative pressure) is connected and the IV bag is disconnected; a 1 second instilling phase when the vacuum is disconnected and the IV hag is connected and the aganocide is applied io the wound; and a 1 minute holding phase when both vacuum and die IV bag are disconnected.
(0075) Each treatment has about 24 cycles and starts with a pumping phase and proceeds for 48 hours. When the treatment is completed, the dressings are changed and the treatment is repeated until full granulation has been promoted and the granulation tissue is within the level of the skin, approximately 30 to 100 days, depending on the depth of the original wound.
[00761 When compared with treatment of a similar wound with vacuum-assisted wound healing without aganocide or treatment of the wound with aganocide alone the combination treatment regimen described herein achieves superior woimd healing results.
Example 4 (Sodium 2-dichloroamino-2-met3iy!propanesulfonate) [90771 A human secondary intention wound approximately 3-4 em in diameter is fitted with a poiyurεthane sponge, pore size 400-600 microns cut io the size of the wound and twice the thickness of the wound and covered with an adhesive film, per instructions on the vacuum system. The outlet is connected to as IV bag (500 ml) with a 0.1% sodium 2-dichioroaτniiio-2-mefhylρropaπesuifonate solution adjusted with HCI Io a pli of about 4, 0.9% NaCL and is elevated to between 0.1 and i meter above the wound. A 1- cm hole is punctured in the adhesive film, and a second adhesive film, is used to cover the hole, with the second outlet connected to the vacuum pump. [00?8| Each cycle consists of three phases: a 2-hour pumping phase when the vacuum ( 125 mm Hg negative pressure) is connected and the IV bag is disconnected; a 5 second instilling phase when the vacuum is disconnected and the IV bag is connected and the aganocide is applied to the wound; and a 30 minute holding phase when both vacuum and the IV hag are disconnected.
10679) Each treatment has about 19 cycles and starts with a pumping phase and proceeds for 48 hours. When the treatment is completed, the dressings are changed and the treatment is repeated until full granulation has been promoted and the granulation tissue is within the level of the skin, approximately 30 to 100 days, depending on the depth of the original wound. føOfiθ] When compared with treatment of a similar wound with vacuum-assisted wound healing without aganocide or treatment of the wound with aganocide alone the combination treatment regimen described herein achieves superior wound healing results. [00811 The entire disclosures of ail patents, patent publications and cited fire references are incorporated herein by reference in their entirety.
Claims
What is claimed:
I. A method tor the treatment of a chronic wound using a wound treatment regimen, the regimen comprising: a) applying reduced pressure to the wound; b) treating the wound with a substantially non-toxic, non-irritating inorganic or organic agsnocide composition while applying reduce pressure to tlic wound; c) optionally, treating the wound with saline after at least one aganocide treatment step, wherein steps a), b) and c) together comprising a regimen cycle; and d) optionally, repeating at bast one additional treatment regimen cycle.
2. The method of Claim 1, wherein the inorganic or organic aganocide composition Is selected from the group consisting of nypochlσrous acid, a source of hypoehiorotis acid, an N-halo amino acid, an N,N~dihaloamino acid, a source of an N- halo amino acid, a source of an KN-dihaioamino acid and mixtures thereof, 3. The method of Claim 1 , wherein the treatment of the aganocide to the wound is performed before the application of reduced pressure to the wound.
4. The method of Claim 1, wherein the aganocide treatment of the wound is done efter a first application of reduced pressure to the wound.
5. The method of Claim 1 , wherein the saline treatment is performed In conjunction with applying the reduced pressure.
6. The method of Claim 2, wherein the aganocide treatment and the application of reduced pressure is done intermittently.
7. The method of Claim 1 , wherein each aganocide treatment step is followed by a reduced pressure application step, followed by a second aganocide treatment step, followed by a second reduced pressure application step, this sequence of steps being considered a single treatment cycle.
8. The method of Claim 7, comprising a single treatment cycle or at least two single treatment cycles.
9. The method of Claim 7. comprising at least four, six, ten, twelve or 24 but not more than 48 single treatment cycles,
10. The method of Claim 1 , wherein the aganocide is an N-liaio amino acid or an N,N-di haloarai no aci d.
! 1. The method of Claim 1 , therein the treatment with ihe aganoeide is nerfornied b> an automated delivery system.
12. The method of Claim 10, wherein ihe N-haiυ amino acid or an WN-- iihaloamino acid composition has a pi I from about 3,5 to 7. 13. A method for the treatment of a chronic wound using a wound treatment regimen, the regimen comprising: a) treating the wound with at least one application of a substantially non-toxic. non-irritating inorganic or organic aganocide composition: b^ treating ihe wound with saline; and c) applying reduced pressure to the wound.
14. The method of Claim 13, wherein ihe inorganic or organic aganocide composition is selected from the group consisting of hypochlorous acid, a source of h>pochlorou5 acid, an N-halo amino acid, an N.N-dihaiαamino acid, a source of an N- halo amino acid, a source of an NLNkimaloamino acid and mixtures thereof. 15, The method of Claim 13. wherein the treatment of fee aganoeide to the wound is performed before the application of reduced pressure to the wound. i 6. The method of Claim 13. w herein the aganoeide treatment of the wound JS done after a first application of reduced pressure to the wound.
' ". The method of Claim 13, wherein the saline treatment is performed in conjunction w ilh applying the reduced pressure.
: 8. The method of Claim 14, wherein aganocide treatment and the application of reduced pressure is done isitεrmsttenljy,
? 9. Ihe method of Claim 13, wherein each aganoeide treatment step is followed by a reduced pressure application step, followed by a second aganocide treatment i-tep, followed by a second reduced pressure application step, this sequence of steps being considered a single treatment cycle,
20. The method of Claim 19. comprising u single treatment cycle or at least two
MTiglc treatment cycles.
? i The method of Claim 19. comprising at least four, six, ten, twelve or 24 but not more than 48 single treatment cycles.
22, The method of Claim 13, wherein the treatment of the wound with saline is εpphed after at least one aganoeide treatment and one reduced pressure application
>tcp.
23, A method for promoting wound healing of a mammalian patient having a wound using a treatment regimen, wherein the regimen comprises: a) a short term application comprising contacting the wound with a cell- compatible inorganic or organic agaπocide composition in an amount effective to promote wound healing; b) a long term application comprising contacting the wound with an effective amount of the aganocidε composition to promote wound healing, wherein the short term application and the long term application are performed at alternating time intervals to promote wound healing and appropriate to the patient in need of wound care; and c) applying reduced pressure to the wound for a sufficient period of time to promote wound healing.
9 {,41. The method of Claim 23, wherein the aganocidε composition is selected from the group consisting of hypochlorous acid, a source of hypochlorous acid, an N-haio amino acid, an N.N-dibaloamino acid, & source of an N-halo amino acid, a source of an N,N-diha3oamino acid, and a compound of the formula
A-C(R- R°}R(CH2VCCΥZ)-X! (I) or a derivative thereof, or mixtures thereof, wherein A is hydrogen, HaINB- or HaIzN- wherein Ha! is halogen selected from the group consisting of chloro and hromo; R is a carbon single bond or a divalent eyeloalkylene radical with three to six carbon atoms, R! is hydrogen, lower alkyl or the group -COOH; R" is hydrogen or lower alky I; u is 0 or an integer from 1 Io 13, or R! and R0 together with the carbon atom to which they attach form a (CrG;)cycloa[ky[ ring; Y is hydrogen, lower alkyl or -NHj or -NHaIi; and Z is hydrogen or lower alkyl ; and X' is hydrogen, -COOH. -CONIl?, -SOjH, - SCbNH? or -P(K) ,HOH fe, a salt thereof, a derivative thereof, and mixtures thereof,
25. The method of Claim 23, wherein the short terra and/or long term application of the aganocide to the wound is performed before the application of reduced pressure to the wound.
26. The method of Claim 23, wherein the short term and/or long term application of the aganocide to the wound is done after a first application of reduced pressure to the wound.
27. The method of Claim 23, wherein the saline treatment is performed in conjunction with applying the reduced pressure.
T?
28. The method of Claim 24. wherein short term and/or the long term application of the agaiiocide and the application of reduced pressure is done intermittent]}.
29. The method of Claim 23, wherein each of the short term and/ or each of the Oiig term aganocide application step is followed by a reduced pressure application step, followed h} a second short terra and<or long term aganocide application step, "ollowed by a second reduced pressure application step, this sequence of steps hems; considered a single treatment c>ck.
30. The method ofVlaira 2s), comprising one treatment cycle or at least two single treatment cycles. M . The method of Claim 20, comprising at least four, six, ten, twelve or 24 but not more than 48 single treatment cycles.
32, The method of Claim 23, wherein the organic aganocide is selected from the group consisting of N-chlorotaurinc. N,N-dichlorotaurine. N, K-dichloro-2.?- dimcthytiaurine, a salt thereof, m\ά mixtures thereof. 33 , The method of Claim 2?, wherein the treatment w ith the aganocide is performed by an automated ddhery system,
34. The method of Claim 32, wherem the aganocide composition lias a pH from about 3.5 to 7.
35. The method of Claim 32, v, herein the aganocide further includes HOCl or an I K)Cl source.
36. The method of Claim 32, wherein the amount of aganocide is between about !00 to about HtOO(S Tϊpm, about 200 ppm io about 2,000 ppm. or about 400 ppm to about 1 ,000 ppm.
J 7, The method of Claim 36, % hcxem the amount of I R)Cl is from about 20 ppm to 500 ppm, about 40 ppra to 250 ppm or about 50 ppra to \ 50 ppm.
38, The method of Claim 29, wherein the pH of the aganocide composition ss from about 2 to 'λS, or about 3.5 to 6.5.
39. A wound or ulcer treatment s> stem comprising: s device adapted to applying reduced pressure to a wound comprising an ftdhesrse cover enclosing the wound or ulcer and adapted to maintain reduced pleasure around the wound or ulcer and means for supplying reduced pressure to the wound suitable to facilitate healing of the wound or ulcer, means for connecting and
disconnecting or shutting off the reduced pressure from ihe wound or ulcer and means for collecting wound exudate or removing material from the wound or ulcer; ihe device being associated with means for supplying a liquid aganocide composition selected from the group consisting of hypochSorous acid, a hypochiorous acid source and mixtures thereof to the wound; and the treatment system being adapted to applying the agaπoe ide composition before, during or after applying reduced pressure to ihe wound or ulcer,
40. A wound or ulcer treatment system comprising: a device adapted to applying reduced pressure to a wound comprising, an adhesive cover enclosing the wound or ulcer and adapted to maintain reduced pressure around the wound or ulcer and means for supplying reduced pressure to the wound suitable to facilitate healing of the wound or isker. means for connecting, disconnecting, or shutting off the reduced pressure from the wound or ulcer and means for collecting wound exudate or removing material from the wound or ulcer; the device being associated with means for supplying a liquid aganocide composition selected from the group consisting of an N~halo amino acid, an N,N- dihalo amino acid, a salt of an N-faalo amino acid, an N,N-dihalo amino acid, an N- haio amino acid source, an N,N-diha!o amino acid source, a salt thereof, and mixtures thereof to the wound; and the treatment system being adapted to applying the aganoeide composition before, during or afler applying reduced pressure to the wound or uicer.
41. The treatment system, of Claim 40 wherein the liquid aganocide further includes hypochiorous acid, a hypochlorous acid source or mixtures thereof,
42. The treatment system of Claim 39, 40 or 41 wherein the means for supplying the liquid aganocide composition are connected to the adhesive cover separately from
LHe means for connecting, disconnecting, or shutting off the reduced pressure from the wound or ulcer and the means for collecting wound exudate or removing material from the wound or ulcer.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002655557A CA2655557A1 (en) | 2006-06-14 | 2007-06-14 | Method for treatment of wound using aganocides |
EP07798590A EP2037908A2 (en) | 2006-06-14 | 2007-06-14 | Method for treatment of wound using aganocides |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US81380506P | 2006-06-14 | 2006-06-14 | |
US60/813,805 | 2006-06-14 |
Publications (2)
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WO2007147085A2 true WO2007147085A2 (en) | 2007-12-21 |
WO2007147085A3 WO2007147085A3 (en) | 2008-02-07 |
Family
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Family Applications (1)
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PCT/US2007/071263 WO2007147085A2 (en) | 2006-06-14 | 2007-06-14 | Treatment of wound using aganocides and negative pressure |
Country Status (4)
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US (2) | US20070292489A1 (en) |
EP (1) | EP2037908A2 (en) |
CA (1) | CA2655557A1 (en) |
WO (1) | WO2007147085A2 (en) |
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EP2081629A2 (en) * | 2006-09-28 | 2009-07-29 | Puricore Inc. | Apparatus and method for wound, cavity, and bone treatment |
WO2009096993A1 (en) * | 2008-02-01 | 2009-08-06 | Alcon Research, Ltd. | Antimicrobial n-halogenated amino acid salts |
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US7393522B2 (en) * | 2000-01-12 | 2008-07-01 | Novabay Pharmaceuticals, Inc. | Physiologically balanced, ionized, acidic solution and methodology for use in wound healing |
GB0224986D0 (en) | 2002-10-28 | 2002-12-04 | Smith & Nephew | Apparatus |
GB0325120D0 (en) | 2003-10-28 | 2003-12-03 | Smith & Nephew | Apparatus with actives |
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US11298453B2 (en) | 2003-10-28 | 2022-04-12 | Smith & Nephew Plc | Apparatus and method for wound cleansing with actives |
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US8758313B2 (en) | 2003-10-28 | 2014-06-24 | Smith & Nephew Plc | Apparatus and method for wound cleansing with actives |
US7909805B2 (en) | 2004-04-05 | 2011-03-22 | Bluesky Medical Group Incorporated | Flexible reduced pressure treatment appliance |
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CA2655557A1 (en) * | 2006-06-14 | 2007-12-21 | Novabay Pharmaceuticals, Inc. | Method for treatment of wound using aganocides |
US8877257B2 (en) * | 2007-01-16 | 2014-11-04 | Puricore, Inc. | Methods and compositions for treating conditions associated with infection and/or inflammation |
US9999635B2 (en) | 2007-01-16 | 2018-06-19 | Realm Therapeutics, Inc. | Methods and compositions for treating inflammatory disorders |
WO2008094663A2 (en) * | 2007-01-31 | 2008-08-07 | Adam Heller | Electrochemical management of pain |
US9023001B2 (en) * | 2007-09-12 | 2015-05-05 | Heal-Ex, Llc | Systems and methods for providing a debriding wound vacuum |
US8460258B2 (en) * | 2008-01-08 | 2013-06-11 | Southeastern Medical Technologies, Llc | Methods and apparatuses for the treatment of wounds with pressures altered from atmospheric |
WO2009127924A1 (en) * | 2008-04-15 | 2009-10-22 | Waldemar Gottardi | Compositions and devices for antisepsis and anticoagulation |
US9381214B2 (en) | 2011-03-18 | 2016-07-05 | Puricore, Inc. | Methods for treating skin irritation |
US11814739B2 (en) | 2016-06-09 | 2023-11-14 | De Nora Holdings Us, Inc. | Electrolytic production of organic chloramine solutions |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1993009727A1 (en) * | 1991-11-14 | 1993-05-27 | Wake Forest University | Method and apparatus for treating tissue damage |
WO2001054704A1 (en) * | 2000-01-12 | 2001-08-02 | Novacal Pharmaceuticals, Llc | Physiologically balanced, ionized, acidic solution and methodology for use in wound healing |
WO2002022118A1 (en) * | 2000-09-14 | 2002-03-21 | Waldemar Gottardi | Fungicidal agent containing n-chlorotaurine and use thereof |
US20050065115A1 (en) * | 2003-08-18 | 2005-03-24 | Mansour Bassiri | N,N-dihalogenated amino acids and derivatives |
WO2007044559A1 (en) * | 2005-10-06 | 2007-04-19 | Novabay Pharmaceuticals, Inc. | System and method for the prevention of bacterial and fungal infections including urinary tract infections (uti) using n-halogenated amino acids |
Family Cites Families (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4908215A (en) * | 1986-03-06 | 1990-03-13 | Brandeis University | Hypochlorite compositions containing thiosulfate and use thereof |
GB8717235D0 (en) * | 1987-07-21 | 1987-08-26 | Darling Products Ltd J E | Moisture leak alarm |
US5090410A (en) * | 1989-06-28 | 1992-02-25 | Datascope Investment Corp. | Fastener for attaching sensor to the body |
US5622848A (en) * | 1990-05-23 | 1997-04-22 | Medical Discoveries, Inc. | Electrically hydrolyzed salines as microbiocides for in vitro treatment of contaminated fluids containing blood |
US5636643A (en) * | 1991-11-14 | 1997-06-10 | Wake Forest University | Wound treatment employing reduced pressure |
US5427667A (en) * | 1992-04-03 | 1995-06-27 | Bakhir; Vitold M. | Apparatus for electrochemical treatment of water |
US5790036A (en) * | 1992-07-22 | 1998-08-04 | Health Sense International, Inc. | Sensor material for use in detection of electrically conductive fluids |
US5422126A (en) * | 1993-03-24 | 1995-06-06 | Great Lakes Chemical Corporation | Halogen compositions for water treatment and method of preparation thereof |
US6207201B1 (en) * | 1993-06-03 | 2001-03-27 | Amuchina International, Inc. | Sodium hypochlorite based disinfectant and sterilizer for medical-surgical instruments |
JPH08252310A (en) * | 1995-01-17 | 1996-10-01 | Miura Denshi Kk | Washing and disinfecting method of artificial dialyzing device using electrolytically generated acidic water, and device therefor |
US5579765A (en) * | 1995-05-30 | 1996-12-03 | Cox; Danny L. | Monitor to detect bleeding |
US5641520A (en) * | 1995-06-07 | 1997-06-24 | Great Lakes Chemical Corporation | Halogen compositions for water treatment |
US5891074A (en) * | 1996-08-22 | 1999-04-06 | Avitar, Inc. | Pressure wound dressing |
RU2119802C1 (en) * | 1996-12-18 | 1998-10-10 | Стерилокс Текнолоджиз, Инк. | Device for performing electrochemical treatment of liquid media |
US5879292A (en) * | 1997-10-09 | 1999-03-09 | Edward A. Sternberg | Bandage including data acquisition components |
US5879290A (en) * | 1997-10-20 | 1999-03-09 | Bioplexus Corporation | Apparatus for holding intestines out of an operative field |
JP2000042556A (en) * | 1998-05-28 | 2000-02-15 | Shimadzu Corp | Electrolytic water production device |
US6283938B1 (en) * | 1999-05-04 | 2001-09-04 | Mcconnell Daniel E. | Medicating bandage and controllable permeable membrane |
DE19929328A1 (en) * | 1999-06-26 | 2001-01-04 | Daimlerchrysler Aerospace Ag | Device for long-term medical monitoring of people |
GB2355190B (en) * | 1999-08-23 | 2004-07-28 | Sterilox Medical | Improvements in or relating to sterilising preparations |
US6333054B1 (en) * | 1999-10-21 | 2001-12-25 | Amuchina S.P.A. | Topical, non-cytotoxic, antimicrobial hydrogel with thixotropic properties |
US7393522B2 (en) * | 2000-01-12 | 2008-07-01 | Novabay Pharmaceuticals, Inc. | Physiologically balanced, ionized, acidic solution and methodology for use in wound healing |
US20030185704A1 (en) * | 2000-01-12 | 2003-10-02 | Suzanne Bernard | Physiologically balanced, ionized, acidic solution and methodology for use in wound healing |
CA2655557A1 (en) * | 2006-06-14 | 2007-12-21 | Novabay Pharmaceuticals, Inc. | Method for treatment of wound using aganocides |
US7815411B2 (en) * | 2007-05-11 | 2010-10-19 | Larry Alexander | Multiple function landscaping system and method |
-
2007
- 2007-06-14 CA CA002655557A patent/CA2655557A1/en not_active Abandoned
- 2007-06-14 US US11/763,368 patent/US20070292489A1/en not_active Abandoned
- 2007-06-14 EP EP07798590A patent/EP2037908A2/en not_active Withdrawn
- 2007-06-14 US US11/763,362 patent/US20070292488A1/en not_active Abandoned
- 2007-06-14 WO PCT/US2007/071263 patent/WO2007147085A2/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1993009727A1 (en) * | 1991-11-14 | 1993-05-27 | Wake Forest University | Method and apparatus for treating tissue damage |
WO2001054704A1 (en) * | 2000-01-12 | 2001-08-02 | Novacal Pharmaceuticals, Llc | Physiologically balanced, ionized, acidic solution and methodology for use in wound healing |
WO2002022118A1 (en) * | 2000-09-14 | 2002-03-21 | Waldemar Gottardi | Fungicidal agent containing n-chlorotaurine and use thereof |
US20050065115A1 (en) * | 2003-08-18 | 2005-03-24 | Mansour Bassiri | N,N-dihalogenated amino acids and derivatives |
WO2007044559A1 (en) * | 2005-10-06 | 2007-04-19 | Novabay Pharmaceuticals, Inc. | System and method for the prevention of bacterial and fungal infections including urinary tract infections (uti) using n-halogenated amino acids |
Non-Patent Citations (4)
Title |
---|
"Perspectives in wound care" THE KREMLIN PAPERS, [Online] 2004, pages 1-17, XP002455813 Retrieved from the Internet: URL:http://www.blueskymedical.com/Portals/0/LBL-00221-01-AA%20Reprint,%20Kremlin%20Papers.pdf> [retrieved on 2007-10-12] * |
ANONYMOUS: "V.A.C.® INSTILL? DUAL THERAPIES. ONE ADVANCED WOUND HEALING SYSTEM." V.A.C. THERAPY UNIT BROCHURES, [Online] 2005, XP002455812 Retrieved from the Internet: URL:www.kci1.com/29-A-142_Instill_Brochure _12-05.pdf> [retrieved on 2007-10-12] * |
ARGENTA L C ET AL: "Vacuum-assisted closure: A new method for wound control and treatment: Clinical experience" ANNALS OF PLASTIC SURGERY 1997 UNITED STATES, vol. 38, no. 6, 1997, pages 563-577, XP009090817 ISSN: 0148-7043 * |
KATHY DIX: "Wound care" INFECTION CONTROL TODAY MAGAZINE, [Online] 5 January 2006 (2006-01-05), XP002455814 Retrieved from the Internet: URL:www.infectioncontroltoday.com/articles /651feat5.html> [retrieved on 2007-10-19] * |
Cited By (3)
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EP2081629A2 (en) * | 2006-09-28 | 2009-07-29 | Puricore Inc. | Apparatus and method for wound, cavity, and bone treatment |
EP2081629A4 (en) * | 2006-09-28 | 2014-06-25 | Puricore Inc | Apparatus and method for wound, cavity, and bone treatment |
WO2009096993A1 (en) * | 2008-02-01 | 2009-08-06 | Alcon Research, Ltd. | Antimicrobial n-halogenated amino acid salts |
Also Published As
Publication number | Publication date |
---|---|
EP2037908A2 (en) | 2009-03-25 |
WO2007147085A3 (en) | 2008-02-07 |
US20070292489A1 (en) | 2007-12-20 |
CA2655557A1 (en) | 2007-12-21 |
US20070292488A1 (en) | 2007-12-20 |
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