WO2007103521A3 - Preventing norvaline and norleucine misincorporation in recombinant proteins - Google Patents
Preventing norvaline and norleucine misincorporation in recombinant proteins Download PDFInfo
- Publication number
- WO2007103521A3 WO2007103521A3 PCT/US2007/005963 US2007005963W WO2007103521A3 WO 2007103521 A3 WO2007103521 A3 WO 2007103521A3 US 2007005963 W US2007005963 W US 2007005963W WO 2007103521 A3 WO2007103521 A3 WO 2007103521A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- norvaline
- preventing
- recombinant proteins
- norleucine misincorporation
- misincorporation
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Accommodation For Nursing Or Treatment Tables (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Methods of preventing norvaline and norleucine misincorporation in recombinant proteins.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP07752647A EP1996697A2 (en) | 2006-03-07 | 2007-03-07 | Preventing norvaline and norleucine misincorporation in recombinant proteins |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US77942906P | 2006-03-07 | 2006-03-07 | |
US60/779,429 | 2006-03-07 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007103521A2 WO2007103521A2 (en) | 2007-09-13 |
WO2007103521A3 true WO2007103521A3 (en) | 2008-02-21 |
Family
ID=38475570
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2007/005963 WO2007103521A2 (en) | 2006-03-07 | 2007-03-07 | Preventing norvaline and norleucine misincorporation in recombinant proteins |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP1996697A2 (en) |
WO (1) | WO2007103521A2 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
LT3597659T (en) | 2007-07-09 | 2023-05-10 | Genentech, Inc. | Prevention of disulfide bond reduction during recombinant production of polypeptides |
JP6479661B2 (en) * | 2012-09-19 | 2019-03-06 | ジェネンテック, インコーポレイテッド | Methods and compositions for preventing norleucine misincorporation into proteins |
JP2022514083A (en) * | 2018-12-20 | 2022-02-09 | サノフィ-アベンティス・ドイチュラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | How to reduce misuptake of non-canonical branched chain amino acids |
WO2024079114A1 (en) | 2022-10-11 | 2024-04-18 | UCB Biopharma SRL | Process for the production of recombinant proteins |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050037475A1 (en) * | 2003-08-13 | 2005-02-17 | Chiron Corporation | Method of purifying TFPI and TFPI analogs |
WO2005038017A2 (en) * | 2003-09-25 | 2005-04-28 | Monsanto Technology Llc | Prevention of incorporation of non-standard amino acids into protein |
-
2007
- 2007-03-07 EP EP07752647A patent/EP1996697A2/en not_active Withdrawn
- 2007-03-07 WO PCT/US2007/005963 patent/WO2007103521A2/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050037475A1 (en) * | 2003-08-13 | 2005-02-17 | Chiron Corporation | Method of purifying TFPI and TFPI analogs |
WO2005038017A2 (en) * | 2003-09-25 | 2005-04-28 | Monsanto Technology Llc | Prevention of incorporation of non-standard amino acids into protein |
Non-Patent Citations (3)
Title |
---|
APOSTOL IZYDOR ET AL: "Incorporation of norvaline at leucine positions in recombinant human hemoglobin expressed in Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 46, 14 November 1997 (1997-11-14), pages 28980 - 28988, XP002461320, ISSN: 0021-9258 * |
BOGOSIAN G ET AL: "Biosynthesis and incorporation into protein of norleucine by Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 264, no. 1, 5 January 1989 (1989-01-05), pages 531 - 539, XP002324310, ISSN: 0021-9258 * |
TSAI L B ET AL: "CONTROL OF MISINCORPORATION OF DE-NOVO SYNTHESIZED NORLEUCINE INTO RECOMBINANT INTERLEUKIN-2 ESCHERICHIA-COLI", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 156, no. 2, 1988, pages 733 - 739, XP009093276, ISSN: 0006-291X * |
Also Published As
Publication number | Publication date |
---|---|
WO2007103521A2 (en) | 2007-09-13 |
EP1996697A2 (en) | 2008-12-03 |
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