WO2007046334A1 - バイオセンサチップ及びバイオセンサチップの製造方法 - Google Patents
バイオセンサチップ及びバイオセンサチップの製造方法 Download PDFInfo
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- WO2007046334A1 WO2007046334A1 PCT/JP2006/320569 JP2006320569W WO2007046334A1 WO 2007046334 A1 WO2007046334 A1 WO 2007046334A1 JP 2006320569 W JP2006320569 W JP 2006320569W WO 2007046334 A1 WO2007046334 A1 WO 2007046334A1
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- WIPO (PCT)
- Prior art keywords
- reaction chamber
- potassium
- biosensor chip
- enzyme
- potassium ferricyanide
- Prior art date
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/002—Electrode membranes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
Definitions
- the present invention relates to a biosensor chip that performs a biochemical reaction with a very small amount of measurement sample and a method for manufacturing the biosensor chip.
- a nanosensor chip introduces a small amount of measurement sample to be measured into a reaction chamber in the biosensor chip, and performs a biochemical reaction such as an enzyme reaction or antigen-antibody reaction on the small amount of measurement sample in the reaction chamber. It is a sensor chip that wakes up and outputs the information obtained through this biochemical reaction via an electrode.
- This biosensor chip utilizes the excellent molecular discrimination function of living organisms, and enables rapid and simple measurement of trace amounts of chemical substances. For example, it can be used as a blood glucose level sensor or urine glucose level sensor for measuring glucose level (blood glucose level) and urine sugar level in blood for home health check (self-care) for self-management and prevention of diabetes.
- this biosensor chip is provided with an electrode system 102 consisting of two striped electrode caps on an electrically insulating substrate 101 in an enzyme sensor 100.
- a reaction layer 103 is tightly fixed to one end side of the electrode system 102, and this reaction layer 103 contains ferricyanium potassium as an example of an electron mediator.
- a mask layer 105 having a window 104 is disposed on the upper surface side of the electrode system 102.
- a spacer 107 having a test solution suction port 106 is disposed on the upper surface side of the mask layer 105.
- a protective layer 108 is disposed on the surface. Therefore, the enzyme sensor 100 is configured by laminating the electrically insulating substrate 101, the electrode system 102, the mask layer 105, the spacer 107, and the protective layer 108.
- Patent Document 2 As another example of a conventional biosensor chip, one described in Patent Document 2 is known.
- a support electrode 201 and a reference electrode 202 are arranged on a first electric insulator 200, and a second electric insulator 203 is arranged on the upper surface side of the electrode.
- This biosensor strip has a cutout 204.
- the reagent 205 is accommodated on the support electrode 201 exposed in the cutout portion 204.
- the reagent 205 contains an enzyme and potassium ferricyanide, and a liquid production reagent is added to the surface of the indicator electrode 201 of the cutout portion 204 and dried.
- an additional cutout portion 206 is included to facilitate electrical connection of the potentiometer to the support electrode 201 and the reference electrode 202.
- Patent Document 1 Japanese Published Patent: Japanese Patent Application Laid-Open No. 2001-311712
- Patent Document 2 Japanese Published Patent: Japanese Patent Publication No. 9-500727
- biosensor chips have been desired to have a small capacity in a reaction chamber in which an enzyme or electron mediator and a measurement sample are mixed and reacted.
- the blood glucose level is measured using the blood of the test subject as a measurement sample
- the blood glucose level can be measured by collecting a small amount of blood, and the blood sampling burden on the test subject can be reduced as much as possible.
- ferricyanium potassium is used as the electron mediator when the reaction chamber of the biosensor chip is made small, the crystal grain size of ferricyanium potassium becomes a problem.
- potassium ferricyanide When a mixed aqueous solution containing potassium ferricyanide is applied to the reaction chamber of the biosensor chip and dried, potassium ferricyanide is likely to be crystallized, resulting in a large crystal grain size. If blood is flowed into the reaction chamber using a biosensor chip containing ferricyanium potassium with a large crystal grain size, there is a possibility that the dissolution of ferricyanium potassium will not proceed rapidly and it will not be possible to measure accurately. is there. In addition, the particle size of potassium ferricyanide contained in the reaction chamber of the biosensor chip is large, small and mixed! /, There may be a difference in the dissolved state of ferricyanium potassium, and the measured value may vary. Furthermore, if ferricyanium potassium having a large crystal grain size is gathered near the inlet of the reaction chamber, blood of the measurement reagent may flow into the reaction chamber.
- An object of the present invention is to have a reaction chamber with a small volume that can be measured with a very small amount of measurement sample, and to arrange ferricyanium potassium with a fine crystal grain size in the reaction chamber so that it can be measured quickly and accurately.
- the object is to provide a nano sensor chip and a method of manufacturing the same.
- a biosensor chip comprises an upper and lower substrate, at least two electrodes disposed on at least one of the upper and lower substrates, and a reaction chamber for performing a chemical reaction.
- the chemical contained in the reaction chamber contains at least an enzyme and potassium ferricyanide, and the volume of potassium ferricyanide accommodated in the reaction chamber is VX when the volume of the reaction chamber is V (L). O. l (mg) or more, and the maximum diameter of the crystal grains of the ferricyanium potassium is 100 ( ⁇ m).
- the maximum diameter of the ferricyan potassium crystal particles is 50 ( ⁇ m) or less.
- the enzyme and the ferricyanium potassium are accommodated in the reaction chamber with a space therebetween.
- the upper and lower substrates are formed of a single sheet, and the upper and lower substrates are formed by bending the single sheet.
- the biosensor chip manufacturing method includes upper and lower substrates, and at least two electrodes are disposed on at least one of the upper and lower substrates, and a chemical reaction occurs between the upper and lower substrates.
- a method for producing a biosensor chip for forming a reaction chamber comprising a step of applying at least an enzyme and potassium ferricyanide as a drug in the reaction chamber, and the volume of the reaction chamber is V (L)
- the ferricyanium potassium contained in the reaction chamber is VX O.l (mg) or more, and the ferricyanide potassium is added so that the maximum diameter of the potassium ferricyanide crystal particles is 100 ( ⁇ m) or less.
- the step of freezing is to take one of a heating step and a mixing step with a poor solvent.
- the biosensor chip manufacturing method according to the present invention uses a single sheet and bends the single sheet to form upper and lower substrates.
- the fine crystal particles of ferricyanium potassium are arranged in the reaction chamber. It can be dissolved evenly and measured accurately with little variation in measurement results.
- Fig. 1 is a biosensor chip according to the present invention, in which (A) is an explanatory view of a lateral force, (B) is an explanatory view showing a lower substrate and an electrode, and (C) is a reaction It is the figure which expanded the chamber.
- FIG. 2 is an explanatory view showing a method of manufacturing a biosensor chip
- (A) is an explanatory view of the lower part
- (B) is an explanatory view of the upper part
- (C) is an explanatory view of the upper part.
- FIG. 6 is an explanatory diagram in which an upper part and a lower part are aligned.
- FIG. 3 is an explanatory view showing another method of manufacturing a biosensor chip
- (A) is an explanatory view of the lower part
- (B) is an explanatory view of the upper part
- (C ) Is an explanatory diagram combining the upper part and the lower part.
- FIG. 4 is an explanatory view showing still another manufacturing method of the biosensor chip, (A) is an explanatory view of the lower part, (B) is an explanatory view of the upper part, C) is an explanatory diagram combining the upper part and the lower part.
- FIG. 5 shows a method of manufacturing a biosensor chip using one insulating substrate sheet, (A) is an explanatory diagram before folding the sheet, and (B) is folding the sheet. It is explanatory drawing after.
- FIG. 6 is a perspective view showing an example of a conventional nano sensor chip.
- FIG. 7 is an explanatory view showing another example of a conventional biosensor chip.
- FIG. 1 shows an example of an embodiment of a biosensor chip according to the present invention.
- the biosensor chip 1 has an insulating lower substrate 2, and two electrodes 3 and 4 are arranged in parallel on the upper surface of the lower substrate 2 with a gap therebetween. (See Fig. 1 (B)).
- a lower spacer 5 is fixed on the electrodes 3 and 4 with an adhesive 6.
- the lower spacer 5 has substantially the same width W as the lower substrate 2 and is arranged with a long !, lower spacer 7 and a short, lower spacer spaced apart from each other!
- a groove 9 is formed.
- an upper spacer 11 is fixed on the lower spacer 5 via an adhesive 10.
- the upper spacer 11 has the same size and thickness as the lower spacer 5, and a long upper spacer 12 and a short upper spacer 13 are arranged with an interval between them.
- An upper groove portion 14 is formed in the upper portion.
- an upper substrate 15 is fixed via an adhesive 16. Therefore, the biosensor chip 1 has a configuration in which the lower substrate 2, the electrodes 3, 4, the lower spacer 5, the upper spacer 11, and the upper substrate 15 are stacked.
- the lower substrate 2 and the upper substrate 15 are configured separately, but may be configured integrally. In other words, it is possible to form a lower substrate and an upper substrate by using a single sheet and bending the sheet into a U-shape.
- the reaction chamber 17 is surrounded by the upper and lower substrates 2 and 15, the upper and lower long spacers 7 and 12, and the upper and lower short spacers 8 and 13, as shown in FIG. .
- the reaction chamber 17 is formed with the upper groove portion 9 and the lower groove portion 14 facing each other, and the volume of the reaction chamber 17 is 0.3 microliter. Torr L) is set below.
- the volume of the reaction chamber 17 is 0.3 L
- the volume of the lower groove 9 can be 0.15
- the volume of the upper groove 14 can be 0.15 L.
- Half of the volume is the volume of each groove.
- the volume of the reaction chamber is not more than 0, preferably not less than 0.2 / z L to 0.3.
- the reaction chamber is 3 L or less, the amount of the measuring reagent is very small, so the measuring reagent can be collected easily.
- the volume of the reaction chamber is 0.3 L or more, the collection load of the measurement reagent, for example, the blood collection load in blood glucose level measurement increases.
- An enzyme 18 is applied to the upper substrate 15 of the reaction chamber 17, and when the measurement reagent flows into the reaction chamber 17, an enzyme reaction or a biochemical reaction such as an antigen-antibody occurs.
- the lower substrate 2 and the electrodes 3 and 4 of the reaction chamber 17 are coated with ferricyanium potassium 19, which is an electron mediator, and this ferricyanium potassium 19 is opposed to the enzyme 18 above at an interval.
- ferricyanium potassium 19 is an electron mediator, and this ferricyanium potassium 19 is opposed to the enzyme 18 above at an interval.
- enzyme 18 and ferricyanium potassium 19 are not mixed, the activity of enzyme 18 can be maintained over a long period of time. Since the volume of the reaction chamber 17 is as small as 0.3 L, it is important to keep the enzyme activity high in order to obtain accurate measurement results with a small amount of measurement reagent.
- the capacity of ferricyanium potassium is also important.
- the capacity of ferricyanium potassium 19 is VX O. 1 milligram. (mg) or more is required.
- the amount of potassium ferricyanide is required to be 0.03 mg or more. If the amount of ferricyanium potassium above V X O. lmg is sufficient, it can sufficiently react with a small amount of measurement reagent, and an accurate measurement result can be obtained.
- the dissolved state of ferricyanium potassium affects the measurement results when a small amount of measurement reagent is introduced.
- the maximum diameter of the crystal grains of ferricyanite potassium must be 100 m or less in order for Felicyanite potassium to dissolve quickly and uniformly.
- Potassium ferricyanide with fine crystal grains with a maximum diameter of 100 m or less can be dissolved quickly and uniformly even in a reaction chamber of 0.3 L or less, and accurate measurement results can be obtained.
- the maximum diameter of the ferricyanium potassium crystal particles is 100 ⁇ m or more, the measurement results may be uneven, and the measurement time may be long. There is.
- the maximum diameter of the crystal grains of ferricyanium potassium is 50 ⁇ m or less, the dissolution is uniformly performed with a very rapid force. Therefore, if it is 50 ⁇ m or less, it is more preferable.
- potassium ferricyanide with a maximum diameter of 100 m or less or 50 m or less is applied by applying an aqueous solution containing ferricyanium potassium to the reaction chamber 17 and then subjecting it to freezing, heating, mixing with a poor solvent, etc. Can be produced.
- FIG. 2 shows an embodiment of a method for manufacturing a nanosensor chip according to the present invention.
- FIG. 2A shows a lower portion 20 including the lower substrate 2 of the nano sensor chip 1. Attach electrodes 3 and 4 to lower substrate 2 by screen printing, etc., and attach lower spacer 5 via adhesive 6. An aqueous solution containing potassium ferricyanide 19 is applied to the lower groove portion 9 formed by the long spacer 7 and the short spacer 8. Thereafter, the lower portion 20 of the bisensor chip 1 is placed in a freezing device 21 to freeze the aqueous solution containing ferricyanium potassium 19.
- the freezing temperature is preferably -20 ° C or lower.
- the lower part 20 may be taken out of the freezer 12 and may be naturally dried or vacuum dried. In this manner, fine crystal particles of potassium ferricyanide can be precipitated by freeze-drying an aqueous solution containing potassium ferricyanide 19. After drying, when crystal grains of potassium ferricyanide 19 were measured, the maximum diameter was 100 m or less. In addition, what was rapidly frozen by the freezing apparatus 21 was measured to measure the ferricyan potassium crystal particles, and the maximum diameter was 50 / zm or less.
- the upper portion 22 including the upper substrate 15 of the biosensor chip 1 is produced.
- Adhesive 16 is applied to the upper substrate 15, and the upper spacer 11 is attached.
- An aqueous solution containing enzyme 18 is applied to the upper groove 14 formed by the long spacer 12 and the short spacer 13. Clothed.
- An example of the enzyme used is glucose oxidase (GOD).
- the upper portion 20 and the lower portion 22 of the biosensor chip 1 are bonded together as shown in FIG.
- the upper groove 9 and the lower groove 14 face each other to form a reaction chamber 17.
- the reaction chamber 17 since the ferricyanide 19 and the enzyme 18 are arranged to face each other with a space between them, the enzyme 18 is not mixed with the potassium ferricyanide 19, so that the activity of the enzyme 18 can be maintained.
- the reaction chamber 17 is formed with a volume of 0.3 L or less, and when the volume of the reaction chamber is V, VX O. lmg or more of Felicyan ⁇ 19 is accommodated, and the maximum diameter of crystal particles is 100 m or less
- FIG. 3 shows another embodiment of the method for manufacturing a biosensor chip according to the present invention.
- the same components as those in the biosensor chip shown in FIG. Then, the detailed explanation is omitted.
- FIG. 3A shows a lower portion 20 including the lower substrate 2 of the biosensor chip 1.
- the electrodes 3 and 4 are attached to the lower substrate 2 by screen printing or the like, and the lower spacer 5 is attached via the adhesive 6.
- An aqueous solution containing ferricyanium potassium 19A is applied to the lower groove portion 9 formed by the long lower spacer 7 and the short lower spacer 8. Thereafter, the lower portion 20 of the nanosensor chip 1 including the lower substrate 2 is placed on the upper surface of the heating device 23 using the heating device 23.
- the heating device 23 is activated and heats the aqueous solution containing the fricyanizing power lithium 19A to evaporate water.
- the upper portion 20 of the biosensor 1 is removed from the heating device 23 and cooled.
- fine crystal particles of potassium ferricyanide can be precipitated.
- crystal grains of ferricyanium potassium 19A were measured, and the maximum diameter was 100 m or less.
- the upper portion 22 including the upper substrate 15 of the biosensor chip 1 is produced.
- Adhesive 16 is applied to the upper substrate 15, and the upper spacer 11 is attached.
- An aqueous solution containing the enzyme 18 was applied to the upper groove portion 14 formed by the long upper spacer 12 and the short upper spacer 13.
- An example of the enzyme used is glucose oxidase (GOD).
- GOD glucose oxidase
- reaction chamber 17 potassium ferricyanide 19A and enzyme 18 are placed facing each other with a force interval, so enzyme 18 does not mix with potassium ferricyanide 19A, so the activity of enzyme 18 can be maintained. .
- the reaction chamber 17 is formed with a volume of 0.3 L or less. When the volume of the reaction chamber is V, ferricyanium potassium 19A of VX O. lmg or more is accommodated, and the maximum diameter of crystal particles is 100 m. O
- FIG. 4 shows still another embodiment of the method for manufacturing a biosensor chip according to the present invention, and the same components as those in the biosensor chip shown in FIG. Detailed description thereof is omitted here.
- FIG. 4A shows a lower portion 20 including the lower substrate 2 of the biosensor chip 1. Attach electrodes 3 and 4 to lower substrate 2 by screen printing, etc., and attach lower spacer 5 via adhesive 6. An aqueous solution containing ferricyanium potassium 19B is applied to the lower groove portion 9 formed by the long spacer 7 and the short spacer 8. Thereafter, ethanol, which is a poor solvent for potassium ferricyanide, is applied so as to be mixed with the aqueous solution. Then, due to the presence of ethanol, ferricyanium potassium is precipitated in a microcrystalline state.
- ethanol which is a poor solvent for potassium ferricyanide
- Such a method is known as a solvent reprecipitation method for precipitating microcrystals.
- a heating device as shown in the embodiment of FIG. 3 is not necessary, and moisture can be evaporated at room temperature.
- any solvent can be used as long as it dissolves well with water and is a poor solvent for potassium ferricyanide.
- One example is acetone.
- With the mixing of ethanol fine crystal grains of ferricyanium potassium 19B precipitate. After evaporation of the solvent, crystal grains of Felicyan ⁇ ⁇ ⁇ potassium 19B were measured, and the maximum diameter was 100 m or less.
- the mixing ratio of water and ethanol was 1: 1 or more, when crystal grains of ferricyanide lithium 19B were measured, fine crystal grains with a maximum diameter of 50 m or less could be precipitated. .
- the upper portion 22 including the upper substrate 15 of the biosensor chip 1 is produced.
- Adhesive 16 is applied to the upper substrate 15, and the upper spacer 11 is attached.
- An aqueous solution containing the enzyme 18 was applied to the upper groove portion 14 formed by the long upper spacer 12 and the short upper spacer 13.
- An example of the enzyme used is glucose oxidase (GOD). wear.
- the aqueous solution containing the enzyme 18 is dried, the upper portion 20 and the lower portion 22 of the biosensor chip 1 are bonded together with the adhesive 10 as shown in FIG.
- the upper groove 9 and the lower groove 14 face each other to form a reaction chamber 17.
- reaction chamber 17 potassium ferricyanide 19B and enzyme 18 are placed facing each other at an interval, so enzyme 18 does not mix with potassium ferricyanide 19B, so that the activity of enzyme 18 can be maintained.
- the reaction chamber 17 is formed with a volume of 0.3 L or less, and when the volume of the reaction chamber is V, the capacity of VX O. lmg or more ferritic potassium 19B is accommodated, and the crystal grain size is 100 m or less. Met.
- fine crystalline particles of potassium ferricyanide are obtained by subjecting potassium ferricyanide to a freezing treatment, a heat treatment, or a treatment mixed with a poor solvent. Can be deposited.
- the maximum diameter of the crystal particle of this ferricyanium potassium, which is the electron mediator, can be reduced to 100 m or less, more preferably 50 m or less, so that a very small amount can be measured within the volume of the 0.3 L reaction chamber. It can be dissolved quickly and uniformly by a constant reagent, and an accurate measurement result can be obtained.
- FIGS. 2 to 4 an example in which a biosensor chip is manufactured by bonding an upper portion including an upper substrate of a biosensor chip and a lower portion including a lower substrate to the present invention is shown.
- the upper substrate and the lower substrate can be manufactured using separate upper and lower substrates without separate bodies.
- FIG. 5 shows an example of manufacturing a biosensor chip using a single sheet substrate.
- this biosensor chip 1A has one insulating sheet substrate 25, and the left side of this sheet substrate 25 is the lower substrate 2A. Long and short lower spacers 7 and 8 are arranged on the lower substrate 2A via two electrodes 3 and 4 and an adhesive.
- a lower groove portion 9 is formed between the long and short lower spacers 7 and 8, and ferricyan potassium 19 (19A, 19B) is accommodated in the lower groove portion 9.
- This potassium ferricyanide 19 (19A, 19B) is crystallized by freezing, heating or mixing with a poor solvent by subjecting an aqueous solution containing potassium ferricyanide 19 (19A, 19B) to the method shown in FIGS. Fine crystal particles with a maximum diameter of 100 m or less are deposited.
- the right side of the insulating sheet substrate 25 is an upper substrate 15A, and long and short upper spacers 12 and 13 are disposed on the upper substrate 15A via an adhesive. Long and short upper spacer 12, 1 An upper groove portion 14 is formed between the three, and an enzyme is accommodated in the upper groove portion 14.
- the method shown in FIG. 2 can be applied as an example of housing the enzyme.
- this single insulating sheet substrate 25 is bent into a U-shape as shown in FIG. 5 (B), and the short spacers 8, 13 and the long spacers 7, 12 are bonded to each other. Laminate through 10.
- the reaction chamber 17 is formed by the upper and lower grooves 9 and 14 facing each other.
- ferritic potassium 19 (19A, 19B) and the enzyme are arranged to face each other at an interval, and the activity of the enzyme is maintained.
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Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
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AU2006305299A AU2006305299A1 (en) | 2005-10-17 | 2006-10-16 | Biosensor chip and process for producing the same |
US11/921,166 US20090020420A1 (en) | 2005-10-17 | 2006-10-16 | Biosensor Chip and Biosensor Chip Production Method |
CA002610092A CA2610092A1 (en) | 2005-10-17 | 2006-10-16 | Biosensor chip and biosensor chip production method |
EP06811833A EP1939612A1 (en) | 2005-10-17 | 2006-10-16 | Biosensor chip and process for producing the same |
NO20074900A NO20074900L (no) | 2005-10-17 | 2007-09-26 | Biosensorbrikke og fremgangsmate ved fremstilling av denne |
Applications Claiming Priority (2)
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JP2005-302330 | 2005-10-17 | ||
JP2005302330A JP2007113915A (ja) | 2005-10-17 | 2005-10-17 | バイオセンサチップ及びバイオセンサチップの製造方法 |
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WO2007046334A1 true WO2007046334A1 (ja) | 2007-04-26 |
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US (1) | US20090020420A1 (ja) |
EP (1) | EP1939612A1 (ja) |
JP (1) | JP2007113915A (ja) |
KR (1) | KR20080067571A (ja) |
CN (1) | CN101163964A (ja) |
AU (1) | AU2006305299A1 (ja) |
CA (1) | CA2610092A1 (ja) |
NO (1) | NO20074900L (ja) |
RU (1) | RU2007135620A (ja) |
WO (1) | WO2007046334A1 (ja) |
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JP4635258B2 (ja) * | 2006-03-02 | 2011-02-23 | 独立行政法人産業技術総合研究所 | バイオセンサー |
CN101650331B (zh) * | 2009-08-27 | 2012-09-05 | 无锡爱康生物科技有限公司 | 一种酶生物电化学传感芯片及其制备方法 |
CN101870452B (zh) * | 2010-07-09 | 2012-09-19 | 上海交通大学 | Mems金属丝生物微电极的制备方法 |
CN107543847A (zh) * | 2017-08-29 | 2018-01-05 | 浙江大学 | 一种抗干扰电化学传感器及其测试方法 |
CN114441616B (zh) * | 2021-12-30 | 2023-02-03 | 广州市赛特检测有限公司 | 一种新冠病毒生物探针在电化学生物传感器上的修饰方法 |
Citations (3)
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JPH0262952A (ja) * | 1988-01-29 | 1990-03-02 | Matsushita Electric Ind Co Ltd | バイオセンサ及びその製造方法 |
JP2003065997A (ja) * | 2001-06-14 | 2003-03-05 | Matsushita Electric Ind Co Ltd | バイオセンサ |
JP2005233917A (ja) * | 2003-07-25 | 2005-09-02 | National Institute Of Advanced Industrial & Technology | バイオセンサおよびその製造方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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DE68924026T3 (de) * | 1988-03-31 | 2008-01-10 | Matsushita Electric Industrial Co., Ltd., Kadoma | Biosensor und dessen herstellung. |
CN100339702C (zh) * | 2002-03-01 | 2007-09-26 | 松下电器产业株式会社 | 生物传感器 |
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2005
- 2005-10-17 JP JP2005302330A patent/JP2007113915A/ja not_active Withdrawn
-
2006
- 2006-10-16 AU AU2006305299A patent/AU2006305299A1/en not_active Abandoned
- 2006-10-16 RU RU2007135620/28A patent/RU2007135620A/ru not_active Application Discontinuation
- 2006-10-16 CA CA002610092A patent/CA2610092A1/en not_active Abandoned
- 2006-10-16 CN CNA2006800130846A patent/CN101163964A/zh active Pending
- 2006-10-16 WO PCT/JP2006/320569 patent/WO2007046334A1/ja active Application Filing
- 2006-10-16 EP EP06811833A patent/EP1939612A1/en not_active Withdrawn
- 2006-10-16 KR KR1020077023688A patent/KR20080067571A/ko not_active Application Discontinuation
- 2006-10-16 US US11/921,166 patent/US20090020420A1/en not_active Abandoned
-
2007
- 2007-09-26 NO NO20074900A patent/NO20074900L/no not_active Application Discontinuation
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0262952A (ja) * | 1988-01-29 | 1990-03-02 | Matsushita Electric Ind Co Ltd | バイオセンサ及びその製造方法 |
JP2003065997A (ja) * | 2001-06-14 | 2003-03-05 | Matsushita Electric Ind Co Ltd | バイオセンサ |
JP2005233917A (ja) * | 2003-07-25 | 2005-09-02 | National Institute Of Advanced Industrial & Technology | バイオセンサおよびその製造方法 |
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AU2006305299A1 (en) | 2007-04-26 |
EP1939612A1 (en) | 2008-07-02 |
CA2610092A1 (en) | 2007-04-26 |
CN101163964A (zh) | 2008-04-16 |
KR20080067571A (ko) | 2008-07-21 |
RU2007135620A (ru) | 2009-03-27 |
JP2007113915A (ja) | 2007-05-10 |
NO20074900L (no) | 2008-07-17 |
US20090020420A1 (en) | 2009-01-22 |
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