WO2006075183A2 - Surface functionalisation using arylcarbene reactive intermediates - Google Patents
Surface functionalisation using arylcarbene reactive intermediates Download PDFInfo
- Publication number
- WO2006075183A2 WO2006075183A2 PCT/GB2006/000139 GB2006000139W WO2006075183A2 WO 2006075183 A2 WO2006075183 A2 WO 2006075183A2 GB 2006000139 W GB2006000139 W GB 2006000139W WO 2006075183 A2 WO2006075183 A2 WO 2006075183A2
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- WIPO (PCT)
- Prior art keywords
- substituted
- unsubstituted
- substrate
- aryl
- compound
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- QYCSNMDOZNUZIT-UHFFFAOYSA-N benzhydrylidenehydrazine Chemical compound C=1C=CC=CC=1C(=NN)C1=CC=CC=C1 QYCSNMDOZNUZIT-UHFFFAOYSA-N 0.000 description 1
- 125000000319 biphenyl-4-yl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1C1=C([H])C([H])=C([*])C([H])=C1[H] 0.000 description 1
- 238000006664 bond formation reaction Methods 0.000 description 1
- 239000007767 bonding agent Substances 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000001721 carbon Chemical group 0.000 description 1
- 229910021387 carbon allotrope Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229940125904 compound 1 Drugs 0.000 description 1
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- 238000004042 decolorization Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-O diazynium Chemical compound [NH+]#N IJGRMHOSHXDMSA-UHFFFAOYSA-O 0.000 description 1
- GTNGBHWYALUTBM-UHFFFAOYSA-N diphenylmethanone;hydrochloride Chemical compound Cl.C=1C=CC=CC=1C(=O)C1=CC=CC=C1 GTNGBHWYALUTBM-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 125000006575 electron-withdrawing group Chemical group 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 125000001301 ethoxy group Chemical group [H]C([H])([H])C([H])([H])O* 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 125000004005 formimidoyl group Chemical group [H]\N=C(/[H])* 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 229910003472 fullerene Inorganic materials 0.000 description 1
- 150000002241 furanones Chemical class 0.000 description 1
- 125000003844 furanonyl group Chemical group 0.000 description 1
- 125000002541 furyl group Chemical group 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 150000002430 hydrocarbons Chemical group 0.000 description 1
- 125000002768 hydroxyalkyl group Chemical group 0.000 description 1
- 125000002883 imidazolyl group Chemical group 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 125000003392 indanyl group Chemical group C1(CCC2=CC=CC=C12)* 0.000 description 1
- 125000003454 indenyl group Chemical group C1(C=CC2=CC=CC=C12)* 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910003480 inorganic solid Inorganic materials 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
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- 239000000543 intermediate Substances 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- OWFXIOWLTKNBAP-UHFFFAOYSA-N isoamyl nitrite Chemical compound CC(C)CCON=O OWFXIOWLTKNBAP-UHFFFAOYSA-N 0.000 description 1
- 125000002510 isobutoxy group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])O* 0.000 description 1
- 125000003253 isopropoxy group Chemical group [H]C([H])([H])C([H])(O*)C([H])([H])[H] 0.000 description 1
- 125000005956 isoquinolyl group Chemical group 0.000 description 1
- 125000001786 isothiazolyl group Chemical group 0.000 description 1
- 125000000842 isoxazolyl group Chemical group 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229960003085 meticillin Drugs 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 125000006606 n-butoxy group Chemical group 0.000 description 1
- 125000003506 n-propoxy group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])O* 0.000 description 1
- 239000002071 nanotube Substances 0.000 description 1
- 125000001624 naphthyl group Chemical group 0.000 description 1
- 239000004763 nomex Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 125000001715 oxadiazolyl group Chemical group 0.000 description 1
- 125000002971 oxazolyl group Chemical group 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000000678 plasma activation Methods 0.000 description 1
- 239000003880 polar aprotic solvent Substances 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 125000003373 pyrazinyl group Chemical group 0.000 description 1
- 125000003072 pyrazolidinyl group Chemical group 0.000 description 1
- 125000003226 pyrazolyl group Chemical group 0.000 description 1
- 125000002098 pyridazinyl group Chemical group 0.000 description 1
- 125000004076 pyridyl group Chemical group 0.000 description 1
- 125000000714 pyrimidinyl group Chemical group 0.000 description 1
- 125000000168 pyrrolyl group Chemical group 0.000 description 1
- 125000005493 quinolyl group Chemical group 0.000 description 1
- 239000000985 reactive dye Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000011268 retreatment Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 229930195734 saturated hydrocarbon Natural products 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 125000005920 sec-butoxy group Chemical group 0.000 description 1
- 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000012312 sodium hydride Substances 0.000 description 1
- 238000012430 stability testing Methods 0.000 description 1
- 125000000547 substituted alkyl group Chemical group 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 125000004213 tert-butoxy group Chemical group [H]C([H])([H])C(O*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 125000001113 thiadiazolyl group Chemical group 0.000 description 1
- 125000000335 thiazolyl group Chemical group 0.000 description 1
- 125000001544 thienyl group Chemical group 0.000 description 1
- 125000004149 thio group Chemical group *S* 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002341 toxic gas Substances 0.000 description 1
- 125000003258 trimethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])[*:1] 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
Definitions
- the present invention relates to a process for the surface functionalisation of a substrate using an arylcarbene as the reactive intermediate.
- the invention in particular relates to a process for the surface functionalisation of a polymeric or an inorganic substrate for producing a substrate which is coloured and/or which has biocidal activity.
- the process of the invention can be used to produce biocidal substrates in which the biocidal activity is regenerable.
- the invention further relates to carbene precursor compounds for use in the surface functionalisation process, and to processes for preparing certain precursor compounds.
- French Patent No. 1 500 512 describes allowing carbenes to come into contact with an organic solid.
- the preferred method for surface modification is to allow the volatilised carbene to come into contact with the polymer.
- Inherent in this approach are limitations: only volatile (i.e. low molecular weight) carbenes, and those stable to relatively high temperatures, are applicable.
- US patent 5,490,983 relates to immobilised N-halainines for disinfectant use.
- the disinfectant properties of these compounds in solution has been known for many years, for instance in water purification and fabrics.
- the formulations of US patent 5,490,983 involve immobilisation of the biocidal agent in polymeric form, and the biocidal activity of the polymers is in principle regenerable.
- one intrinsic problem is that the regeneration technique requires chlorine. This is a toxic gas, and strenuous efforts to minimise the use of this compound are being made across all industry.
- the technique of US patent 5,490,983 is therefore of inherently restricted applicability.
- the present invention provides a process for producing a substrate having a functionalised surface, which process comprises:
- A is an aryl or heteroaryl ring; p is 1, 2, 3, 4 or 5;
- R 1 is selected from hydrogen, aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C 1 -C 10 alkoxy which is substituted or unsubstituted, C 1 -C 10 alkylamino which is substituted or unsubstituted, di(C 1 -C 10 )alkylamino which is substituted or unsubstituted, C 1 -C 1O alkylthio which is substituted or unsubstituted, and C 1 - C 10 alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R 2 ), O or S wherein R 2 is H or C 1 -C 6 alkyl;
- Q is selected from -N(Z 1 )(Z 2 ) and -CH 2 -V-(W-R) n ;
- Z 1 and Z 2 are independently selected from aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C 1 -C 10 alkoxy which is substituted or unsubstituted, Cj-C 1O alkylamino which is substituted or unsubstituted, d ⁇ CrC ⁇ ojalkylamino which is substituted or unsubstituted, C 1 -C 10 alkylthio which is substituted or unsubstituted, and C]-C 1O alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R 2 ), O or S wherein R 2 is H or C 1 -C 6 alkyl;
- V is -alk, -OaIk-, -alk-O- or -OaIk-O-, wherein alk is C 1 -C 10 alkylene;
- W is a functional group of one of the following formulae (a) to (c):
- X is O, S or NH 2 + ;
- R is selected from H, C 1 -C 6 alkyl which is unsubstituted or substituted, aryl which is unsubstituted or substituted and heteroaryl which is unsubstituted or substituted; and n is 1, 2 or 3;
- each of A and B which are the same or different, is an aryl or heteroaryl ring; each of Q and Q 2 , which are the same or different, is -N(Z 1 )(Z 2 ) or -CH 2 -V-(W-R) n , wherein Z 1 , Z 2 , V, W 5 R and n are as defined above for formula (III); and each of p and r, which are the same or different, is 1, 2, 3, 4 or 5; (b) generating a carbene reactive intermediate from the carbene precursor so that it reacts with the substrate to functionalise the surface, thereby yielding an activated substrate; and
- step (c) of the process of the invention as defined above the activated substrate obtained in step (b) is further functionalised.
- functionalise as used in this context is meant the introduction of a, or of another, chemical functional group which exhibits desirable physical or chemical properties, by covalent attachment or by attachment through hydrogen bonding.
- the activated substrate may be further functionalised by treating the activated substrate with a diazonium salt. Additionally or alternatively, the activated substrate may be further functionalised by treating the activated substrate with hydrogen peroxide, preferably aqueous hydrogen peroxide.
- step (c) involves treating the activated substrate obtained in step (b) with a diazonium salt, thereby forming a diazo coupled coloured substrate.
- the diazonium salt may be selected such that the desired colour is obtained, for instance cyan, magenta, yellow, black, red, green, blue or orange.
- the diazonium salt is of type ArN 2 , wherein Ar is substituted or unsubstituted aryl or substituted or unsubstituted heteroaryl, wherein the aryl or heteroaryl ring may be substituted or unsubstituted at any or all positions of the Ar group.
- Ar is substituted or unsubstituted phenyl.
- Examples include fast black K salt [2,5-dimethoxy-4- ((4-nitrophenyl)diazenyl)benzenediazonium chloride] or 4- ⁇ / , ⁇ / -dimethylaminobenzene diazonium chloride.
- step (b) the activated substrate obtained in step (b) may be treated with a diazonium salt which is itself functionalised to confer a desired activity on the substrate.
- step (c) may involve treating the activated substrate in step (b) with a functionalised diazonium salt, the functionality of which confers the desired activity.
- the desired activity may be a particular hydrophobicity or hydrophilicity, for example.
- step (c) of the process as defined above involves treating the activated substrate obtained in step (b) with hydrogen peroxide, to yield a biocidal polymeric substrate.
- step (b) may be treated with both a diazonium salt and hydrogen peroxide.
- step (c) of the process of the invention as defined above may comprise: (d) treating the activated substrate obtained in step (b) with a diazonium salt, thereby forming a diazo coupled coloured substrate, and (e) treating the diazo coupled coloured substrate obtained in step (d) with hydrogen peroxide.
- step (c) of the process of the invention as defined above may comprise: (d') treating the activated substrate obtained in step (b) with a functionalised diazonium salt, whose functionality confers the desired activity, and (e') treating the diazo coupled substrate obtained in (c) with hydrogen peroxide.
- Q of formula (IV) is -N(Z 1 )(Z 2 )
- Q 2 is also -N(Z 1 )(Z 2 ), wherein Z 1 is as defined above and may be the same or different for Q and Q 2
- Z 2 is as defined above and may be the same or different for Q and Q 2 .
- Q of formula (IV) is -CH 2 -V-(W-R) n
- Q 2 is also -CH 2 -V-(W-R) n wherein V, W, R and n are as defined above and may be the same or different for Q and Q 2 .
- Q of formula (III) is -N(Z 1 )(Z 2 )
- Q and Q 2 of formula (IV) are both -N(Z 1 )(Z 2 ), wherein Z 1 is as defined above and may be the same or different for Q and Q 2
- Z 2 is as defined above and may be the same or different for Q and Q 2 .
- Z 1 is C 1 -C 4 alkyl.
- Z 2 is C 1 -C 4 alkyl.
- Z 1 and Z 2 may be the same C 1 -C 4 alkyl.
- p is 1.
- r is 1.
- Q of formula (III) and Q and Q 2 of formula (IV) are both - CH 2 -V-(W-R) n wherein V, W, R and n are as defined above and may be the same or different for Q and Q 2 .
- V is -OaIk-, wherein alk is C 1 -C 10 alkylene.
- W is a functional group of formula (c).
- R is substituted or unsubstituted phenyl.
- n is 1.
- A is phenyl.
- B is phenyl.
- the present invention provides a process for producing a biocidal polymeric substrate, which process comprises:
- diaryl carbene precursor which precursor is selected from: a compound of the following formula (I): ,
- each of A and B which are the same or different, is an aryl or heteroaryl ring; n is an integer of 1 to 3; y is 0 or an integer of 1 to 5; z is 0 or an integer of 1 to 5; provided that y and z are not both 0;
- V is - alk, -O-alk-, -alk-O- or-O-alk-O-, wherein alk is C 1 -C 10 alkylene;
- W is a functional group of one of the following formulae (a) to (c):
- X is O, S or NH 2 + ;
- R is selected from H, C 1 -C 6 alkyl which is unsubstituted or substituted, aryl which is unsubstituted or substituted and heteroaryl which is unsubstituted or substituted; and a compound of formula (II):
- R is (CH 2 ) m N(R 3 )(R 4 ),
- R 3 is C 1 -C 4 alkyl
- R 4 is phenyl, m is an integer of 1 to 4.
- X is N 2;
- the diaryl carbene precursor is a compound of formula (I).
- y is an integer of 1 to 5 and z is an integer of 1 to 5.
- the process of the invention offers significant economic and technical bulk advantages in that only the surface of the polymer is modified, and it is applicable to a diverse range of substrates including but not limited to natural and synthetic polymers and inorganic solids.
- the colouration of the diazo-coupled coloured substrates does not suffer from leaching, and the biocidal activity of the biocidal substrates is regenerable, that is, the polymer can be reactivated multiple times by treatment with a hydroperoxide solution.
- the process of the present invention converts otherwise inert substrates, such as polymers, into activated substrates which possess surface functionality that allows the binding of a biocidal agent by addition of hydrogen peroxide or the addition of a functional group, such as a chromophoric group, by the addition of a diazonium salt.
- a biocidal agent by addition of hydrogen peroxide or the addition of a functional group, such as a chromophoric group, by the addition of a diazonium salt.
- the biocidal activity is regenerable by subsequent retreatment of the activated substrate with fresh hydrogen peroxide.
- the fact that the functionality is confined to the surface of the substrate is advantageous because the bulk of the polymer is effectively sealed in and therefore the bulk properties of the polymer, including mechanical strength, remain unaffected by the biocidal, colour or other properties that are imparted to the surface.
- the substrate treated in accordance with the present invention may be any natural or synthetic substrate which is capable of reaction with a carbene reactive intermediate generated from a diarylcarbene precursor of formula (III) or (IV) as defined above.
- the substrate is typically a natural or synthetic polymer including but not limited to cellulose, polyglycoside, polypeptide polyacrylates, polyacrylics, polyamides, polycarbonates, polyesters, polyethers, polyketones, polyolefms, rubbers, polystyrenics, polysulfones, polyurethanes, polyvinyls and their co-polymers; or an inorganic material including but not limited to silica, alumina, titania, glasses, and allotropes of carbon such as diamond, diamond-like carbon, graphite, fullerenes and nanotubes.
- the molecular weight of the polymeric substrate may be selected according to the particular utility of the end product.
- the polymeric substrate treated in accordance with the present invention has biocidal properties
- the molecular weight of the polymeric substrate may be selected according to the particular utility of the biocidal end product.
- the substrate is selected from homopolymers and copolymers of ethylene or propylene, polyesters, polyamides, polystyrene, polytetrafluoroethylene, nylon, silica, cotton, silk and wool.
- the polymeric substrate is in a form that has a high surface area, for instance a powder or beads.
- the polymer may be a homopolymer or a copolymer, for instance a block copolymer. It may thus be derived from monomeric units which are the same or different.
- the polymer may be modified, for instance by admixture with an inorganic material.
- the substrate may thus comprise both a polymer and an inorganic material, for instance a mixture of a polymer and an inorganic material such as an inorganic filler.
- the substrate may, for example, comprise a mixture of one or more of the polymers referred to above and one or more of the inorganic materials referred to above.
- Modified polymers of this type are suitable for use in, for instance, semiconductor applications. Technical problems can arise when polymers are used as coating or bonding agents with semiconductors, owing to their different behaviour on heating.
- the inclusion of an inorganic filler with a polymer serves to modify the thermal properties of the polymer and make it better suited for use together with a semiconductor.
- the diarylcarbene precursors of formula (I) are novel compounds.
- the present invention therefore further provides a compound of formula (I): wherein each of A and B, which are the same or different, is an aryl or heteroaryl ring; n is an integer of 1 to 3; y is 0 or an integer of 1 to 5; z is 0 or an integer of 1 to 5; provided that z and y are not both 0;
- V is - alk, -O-alk-, -alk-O- or -O-alk-O-, wherein alk is C 1 -C 1O alkylene;
- W is a functional group of one of the following formulae (a) to (c):
- R is selected from H, C 1 -C 6 alkyl which is unsubstituted or substituted, aryl which is unsubstituted or substituted, and heteroaryl which is unsubstituted or substituted; provided that the compound is not 4,4'-bis(iV-acetyl-2-ammoethyl)diphenyldiazomethane.
- z and y are not both 1.
- y is an integer of 1 to 5 and z is an integer of 1
- a C 1 -C 1O alkyl group is an unsubstituted or substituted, straight or branched chain saturated hydrocarbon radical. Typically it is C 1 -C 6 alkyl, for example methyl, ethyl, propyl, butyl, pentyl or hexyl, or C 1 -C 4 alkyl, for example methyl, ethyl, i-propyl, n- propyl, t-butyl, s-butyl or n-butyl.
- alkyl group When an alkyl group is substituted it typically bears one or more substituents selected from C 1 -C 6 alkyl which is unsubstituted, aryl, cyano, amino, C 1 -C 1O alkylamino, di(Ci-C 10 )alkylamino, amido, hydroxy, halo, carboxy, C 1 -Ce alkoxy, haloalkyl, sulfonic acid, sulfhydryl (i.e. thiol, -SH), C 1 -C 1O thioether and sulfonyl.
- substituted alkyl groups include haloalkyl, hydroxyalkyl, aminoalkyl and alkoxyalkyl groups.
- An aryl group is a substituted or unsubstituted, monocyclic or bicyclic aromatic group which typically contains from 6 to 14 carbon atoms, preferably from 6 to 10 carbon atoms in the ring portion. Examples include phenyl, naphthyl, indenyl and indanyl groups. An aryl group is unsubstituted or substituted.
- an aryl group as defined above When an aryl group as defined above is substituted it typically bears one or more substituents selected from C 1 -C 6 alkyl which is unsubstituted, aryl which is unsubstituted, cyano, amino, amido, hydroxy, halo, carboxy, C 1 -C 6 alkoxy, haloalkyl, sulfonic acid and sulfonyl. Typically it carries 0, 1, 2 or 3 substituents.
- alkylene group is unsubstituted or substituted, straight or branched chain saturated divalent hydrocarbon group.
- it is C 1 -C 8 alkylene, for instance C 1 -C 6 alkylene.
- a heteroaryl group is typically a 5- to 10 membered mono- or bicyclic heteroaromatic ring. It is generally a 5- or 6-membered ring, containing at least one heteroatom selected from O, S, N, P, Se and Si. It may contain, for example, 1, 2 or 3 heteroatoms.
- heteroaryl groups include pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, furanyl, thienyl, pyrazolidinyl, pyrrolyl, oxazolyl, oxadiazolyl, isoxazolyl, thiadiazolyl, thiazolyl, isothiazolyl, imidazolyl, pyrazolyl, quinolyl and isoquinolyl.
- a heteroaryl group may be unsubstituted or substituted, for instance, as specified above for alkyl. Typically it carries 0, 1, 2 or 3 substituents.
- amino represents a group of formula -NH 2 .
- C 1 - C 1O alkylamino represents a group of formula -NHR' wherein R' is a C 1 -C 10 alkyl group, preferably a C 1 -C 6 alkyl group, as defined previously.
- I l represents a group of formula -NR 'R' r wherein W and R" are the same or different and represent C 1 -C 10 alkyl groups, preferably C 1- C 6 alkyl groups, as defined previously.
- a C 1 -C 10 alkylthio group is a said C 1 -C 10 alkyl group attached to a thio group.
- a C 1 -C 10 alkoxy group is a said C 1 -C 10 alkyl group attached to an oxygen atom.
- a C 1 -C 6 alkoxy group is a said C 1 -C 6 alkyl group attached to an oxygen atom.
- a C 1 -C 4 alkoxy group is a C 1 -C 4 alkyl group attached to an oxygen atom.
- C 1- C 4 alkoxy groups include, -OMe (methoxy), -OEt (ethoxy), -O(nPr) (n-propoxy), -O(iPr) (isopropoxy), -0(nBu) (n-butoxy), -O(sBu) (sec-butoxy), -O(iBu) (isobutoxy), and -O(tBu) (tert-butoxy).
- Alkylene and alkyl groups may be interrupted by one or more heteroatoms or heterogroups, such as S, O or N(R 2 ) wherein R 2 is H or C 1 -C 6 alkyl.
- the phrase "optionally interrupted” as used herein thus refers to a C 1 -C 10 alkyl group or an alkylene group, as defined above, which is uninterrupted or which is interrupted between adjacent carbon atoms by a heteroatom such as oxygen or sulfur, or a heterogroup such as N(R 2 ) wherein R 2 is H or C 1 -C 6 alkyl.
- a C 1 -C 10 alkyl group such as n-butyl may be interrupted by the heterogroup N(R 2 ) as follows: -CH 2 N(R 2 )CH 2 CH 2 CH 3, -CH 2 CH 2 N(R 2 )CH 2 CH 3 , or -CH 2 CH 2 CH 2 N(R 2 )CH 3 .
- an alkylene group such as n-butylene may be interrupted by the heterogroup N(R 2 ) as follows: -CH 2 N(R 2 )CH 2 CH 2 CH 2 - , -CH 2 CH 2 N(R 2 )CH 2 CH2-, or -CH 2 CH 2 CH 2 N(R 2 )CH 2 -.
- the or each group Q may occupy any available position on ring A.
- the or each group Q and the or each group Q 2 may occupy any available position on rings A and B respectively.
- parameter "p" is 1
- A is mono-substituted at any ring position by Q; for instance, when A is a phenyl group it may be substituted at any of positions 2, 3, 4, 5 and 6.
- parameter "p" is 2
- A is di-substituted at any two positions by Q; for instance, when A is a phenyl group it may be 2,3- 2,4-, 2,5-, 2,6-, 3,4- or 3,5-disubstituted.
- A is tri-substituted at any three positions by Q; for instance, when A is a phenyl group it may be 2,3,4-, 2,4,5- or 3,4,5- tri-substituted.
- parameter "r" is 1, B is mono-substituted at any ring position by Q 2 ; for instance, when B is a phenyl group it may be substituted at any of positions 2, 3, 4, 5 and 6.
- parameter "r" B is di-substituted at any two positions by Q 2 ; for instance, when B is a phenyl group it may be 2,3- 2,4-, 2,5-, 2,6-, 3,4- or 3,5-disubstituted.
- parameter "r" is 3, B is tri-substituted at any three positions by Q ; for instance, when B is a phenyl group it may be 2,3,4-, 2,4,5- or 3,4,5- tri-substituted.
- the or each chain -[CH 2 -V-(W-R) n ] may occupy any available position on rings A and B.
- B is mono-substituted at any ring position by -[CH 2 -V-(W-R) n ]; for instance, when B is a phenyl group it may be substituted at any of positions 2, 3, 4, 5 and 6.
- B is di-substituted at any two positions by -[CH 2 -V-(W-R) n ] for instance, when B is a phenyl group it may be 2,3- 2,4-, 2,5-, 2,6-, 3,4- or 3,5-disubstituted.
- B is tri-substituted at any three positions by -[CH 2 -V-(W-R) n ]; for instance, when B is a phenyl group it may be 2,3,4-, 2,4,5- or 3,4,5- tri-substituted.
- A is mono-substituted at any ring position by -[CH 2 -V-(W-R) n ]; for instance, when A is a phenyl group it may be substituted at any of positions 2, 3, 4, 5 and 6.
- A is di-substituted at any two positions by -[CH 2 -V-(W-R) n ]; for instance, when A is a phenyl group it may be 2,3- 2,4-, 2,5-, 2,6- , 3,4- or 3,5-disubstituted.
- A is tri-substituted at any three positions by -[CH 2 -V-(W-R) n ]; for instance, when A is a phenyl group it may be 2,3,4-, 2,4,5- or 3,4,5- tri-substituted.
- n qualifies the moiety represented by -W-R and thus defines the number of hydrogen-bonding groups W that are present in the compounds of formulae (I), (Ia), (III) and (IV).
- n is 2 or 3
- the moiety -V-(W-R) n in the chain -[CH 2 -V-(W- R) n ] may be represented, for instance, as -(CR' 2 ) P -CR' (W-R) 2 , -0-(CR' 2 ) P -CR' (W-R) 2 , - (CR' 2 )p-C(W-R) 3 or -O-(CR' 2 )p-C(W-R) 3 wherein p is 0 or an integer of 1 to 10 and each R' is, independently, H or C 1 -C 6 alkyl.
- the compound of formula (I) or formula (III) is of the following formula (Ia):
- V 5 W and R are as defined above.
- a preferred example of a carbene precursor compound of formula (III) or (IV) is 1- ⁇ 2-[4-(diazo-phenyl-methyl)-benzyloxy]-ethyl ⁇ -3-phenyl urea, hereafter compound XIII.
- This compound may be produced as shown in the following scheme 1.
- 4- Bromomethylbenzophenone which can be prepared according to D. D. Tanner et ah, J. Org. Chem., 1980, 45, 5177, is first coupled with ethanolamine. The resulting benzophenone may be converted to the urea compound by treatment with phenyl isocyanate in dry dichloromethane.
- the urea compound may be converted to the hydrazone by treatment with hydrazine hydrate in refluxing methanol overnight, followed by removal of the solvent and extraction into dichloromethane.
- Oxidation of the hydrazone to the corresponding diazo diarylcarbene precursor may conveniently be performed using mercuric oxide in ether, or any other suitable oxidant.
- Carbene precursor compounds of formulae (III) or (IV) in which Q and/or Q 2 is - CH 2 - V-(W-R) n , as defined above, may be produced by analogy with the method shown in scheme 1, from starting materials which are commercially available or which may readily be synthesised by known techniques.
- Diarylcarbene precursors of formula (II) are known from WO 00/26180.
- a preferred example of a compound of formula (II) is 4-([iV-ethyl-iV-phenyl-2- . aminoethyl]oxymethyl)phenyl phenyl diazomethane, hereafter compound XVI. This compound may be produced as shown in the following scheme 2:
- a preferred example of a compound of formula (III) or (IV) is bis-4,4'-7 ⁇ iV- dimethylamino diphenyldiazomethane, hereafter compound 3.
- This compound may be produced as shown in the following Scheme 3.
- Michler's ketone (1) can be converted to the known hydrazone (2) by reaction with hydrazine hydrate in ethanol.
- This hydrazone can be oxidised to diazomethane (3) using yellow mercuric oxide in tetrahydrofuran.
- Carbene precursor compounds of formulae (III) or (IV) in which Q and/or Q 2 is -N(Z 1 )(Z 2 ), as defined above, may be produced by analogy with the method shown in scheme 3, from starting materials which are commercially available or which may readily be synthesised by known techniques.
- the present invention further provides a process for producing a compound of formula (VII) or (VIII):
- A is an aryl or heteroaryl ring
- B is an aryl or heteroaryl ring; p is 1, 2, 3, 4 or 5; r is 1, 2, 3, 4 or 5;
- R 1 is selected from hydrogen, aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C 1 -C 10 alkoxy which is substituted or unsubstituted, C 1 -Cj 0 alkylamino which is substituted or unsubstituted, di(C 1 -Cio)alkylamino which is substituted or unsubstituted, Ci-C 10 alkylthio which is substituted or unsubstituted, and C 1 - C 10 alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R 2 ), O or S wherein R 2 is H or C 1 -C 6 alkyl;
- Q 2 which may be the same as or different from Q, is -N(Z 1 )(Z 2 );
- Z 1 and Z 2 are independently selected from aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C 1 -C 10 alkoxy which is substituted or unsubstituted, C 1 -Ci 0 alkylamino which is substituted or unsubstituted, di(Ci-Cio)alkylamino which is substituted or unsubstituted, C 1 -C 1O alkylthio which is substituted or unsubstituted, and C 1 -C 10 alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R ), O or S wherein R is H or C 1 -C 6 alkyl; the process comprising treating a compound of formula (V) or (VI) with hydrazine in the presence of heat and a solvent:
- A, B, Q, Q 2 , p, r and R 1 are as defined above.
- hydrazine is used in the form of hydrazine hydrate.
- Any suitable solvent is employed, for instance a polar protic solvent such as an alcohol.
- the solvent is ethanol.
- the reaction is carried out with heating, typically at the reflux temperature of the solvent used.
- the solvent is ethanol the reaction is suitably carried out at a temperature of 78 °C or higher, e.g. at a temperature of 80 °C.
- the process as defined above further comprises oxidising a compound of formula (VII) or (VIII) to produce a carbene precursor compound of formula (III) or (IV) respectively:
- A, B, Q, Q 2 , p, r and R 1 are as defined above.
- any suitable oxidant may be used.
- the oxidant is mercuric oxide in an aprotic polar solvent, for instance tetrahydrofuran (THF) or an ether. More typically, this oxidation is conducted in the presence of a base, for instance a metal hydroxide and sodium sulphate.
- the metal hydroxide is typically an alkali metal hydroxide, for instance potassium hydroxide.
- a saturated solution of the metal hydroxide is generally used.
- the solvent used for the metal hydroxide is suitably a polar protic solvent such as an alcohol, for instance ethanol.
- the solvent used for the solution of the compound of formula (VII) or (VIII) is suitably a polar aprotic solvent, for instance tetrahydrofuran.
- the carbene precursor compound of formula (III) or (IV) thus produced may be employed as the carbene precursor in step (a) of the process of the invention for producing a substrate having a functionalised surface, as defined above.
- carbene reactive intermediate is meant a reactive species comprising a formally divalent carbon atom.
- the carbene reactive intermediate is generated from the carbene precursor of formula (III) or (IV) by treatment under conditions which result in an irreversible covalent reaction with the substrate.
- the carbene is generated by heating or irradiating the substrate and pre-adsorbed carbene precursor compound; since the carbene precursor of formula (II) or (IV) is usually intensely coloured (red-orange), the formation and insertion of the carbene is most conveniently monitored by decolourisation.
- the coloured substrates produced by the process of the present invention may be used in the dye industry for the preparation of non-leaching dyed polymers, such an example would be in food and drink packaging; they may be used to confer specific wavelength absorbing properties such as ultraviolet or infrared absorption; the ability of the substrates to show photochromism could be utilised in the medical market for contact lenses; the introduction of fluorescent markers allows security tagging applications.
- the coloured substrates produced by the present invention are capable of being irreversibly dyed with little or no leaching from the surface, which is an important practical advantage associated with their use.
- biocidal polymeric substrates produced by the process of the present invention may be used in many different fields, but they particularly have utility in any context where the occurrence of bacterial, viral or fungal infection is a risk. For instance, they may have medical, industrial, catering, domestic, public health or military applications. Hydrogen peroxide-based biocidal agents are tasteless and odourless and are therefore preferable in many contexts to chlorine-based biocides.
- biocidal polymeric substrates are effective against a wide range of microorganisms including Gram-positive and Gram-negative bacteria, as well as viruses.
- bacteria include S. Aureus and E. CoIi.
- biocidal polymeric substrates produced by the process of the present invention include use in water purification systems, both small-scale and industrial; use in water filtration systems, for instance water filter cartridges for domestic and military use, in emergency applications and disaster relief; use in air purification systems, for instance air recirculation systems in aircraft, breathing apparatus and environmental protection products; use in medical and military equipment and clothing such as surgical gowns, surgical masks and surgical instruments; and use in medical devices such as implants, stents and catheters.
- biocidal polymeric substrates produced by the process of the present invention are capable of being regenerated on multiple occasions after use by treatment with hydrogen peroxide, which is an important practical advantage associated with their use. Regeration may be achieved by simple re- washing of the substrate with aqueous hydrogen peroxide.
- the process is extendable from chromophoric and biocidal applications to include other chemical and reactive functionality.
- the process may be used to produce a substrate having a surface of a particular hydrophobicity or hydrophilicity, for example.
- Example 1 Preparation of l- ⁇ 2-[4-(Diazo-phenvI-methvI)-benzyIoxy1-ethyI ⁇ -3- phenyl urea (XIII)
- Example 2 Preparation of functionalised polymer beads/powder.
- a sample of the required polymer was suspended in 50% aqueous hydrogen peroxide for 1 hour and the polymer was collected by filtration and washed with 1-1.51 of water, collected and treated with a solution of 10% potassium iodide in aqueous acetic acid. After standing for 5 minutes, a 1% aqueous starch solution was added, and the suspension left to stand for 1 hr. The resulting dark blue colour solution was titrated with sodium thiosulfate until the colourless endpoint. The mixture was left for a further hour and any further blue coloration of the solution was further titrated with sodium thiosulfate.
- the polymer was collected by filtration and washed with 1-1.51 of water, collected in a stoppered vial and stored at room temperature in a darkened cupboard. At regular time intervals 10 mg samples were collected and tested for the hydrogen peroxide loading using the above procedure.
- a sample of the required polymer (0.05g) was suspended in 50% aqueous hydrogen peroxide (2ml) for 18 h.
- the polymer was collected by filtration and washed with 1-1.51 of water, collected and treated with solution of 10% potassium iodide in acetic acid and water. After standing for 5 minutes a 1% aqueous starch solution was added and the suspension left to stand for Ih.
- a positive test for hydrogen peroxide was the formation of a dark blue coloured solution. This mixture was then quenched with sodium thiosulfate until colourless and the polymer filtered and washed with 1-1.51 of water. The polymer was then re-suspended in fresh 50% aqueous hydrogen peroxide (2ml) and the cycle repeated.
- agar plate inoculated with the chosen organism was punched with 10mm wells. To these wells was added 20 ⁇ l of molten agar. When set the polymer sample was added to the well so that a thin layer of polymer was produced. The well was then sealed with a further 50 ⁇ l of molten agar, the plate incubated overnight at 37°C and the diameter of the inhibition zones measured.
- Table 5 Zones of inhibition results against S. Aureus using 10 mm wells in Hole-Plate
- Table 7 a Ratio of diazomethane to polymer used during functionalisation step.
- Solvent used for diazo absorption onto the polymer. c Amberlite XAD-4.
- Cross Linked Polystyrene. e Ultra High Molecular Weight Polyethylene.
- f Surface Modified Ultra High Molecular Weight Polyethylene g Polyethylene. h Polypropylene. ' Nylon-610. j Normal Knitted Cotton. k Mercerised Woven Cotton. ' Poly(ethylene terephthalate).
- Polymers 4a-r were dyed by immersing the functionalised polymer in a 0.1 M solution of fast black K salt 5 in acetone for 18h. The polymer was then collected by filtration and washed with acetone to furnish the corresponding diazo polymers 6a-r (Table 2, Scheme 5).
- p ) XAD-4 6a
- Tencel 6k ⁇ PSXL-S 6b Viscose 61 UHMWPE 6c NK-Cotton 6m SMUHMWPE 6d MW-Cotton 6n PE-S 6e Cotton Wool 6o PP 6f PET 6p PP-S 6g Silica Gel 6q N-610 6h Alumina 6r Hybond-N 6i Kevlar 6j
- Example 10 Binding of hydrogen peroxide to functionalised polymers (9a-s, 10)
- ⁇ p) XAD-4 9a
- Tencel 9k PSXL-S 9b Viscose 91 UHMWPE 9c NK-Cotton 9m SMUHMWPE 9d MW-Cotton 9n PE-S 9e Cotton Wool 9o PP 9f PET 9p PP-S 9g Silica Gel 9q N-610 9h Alumina 9r Hybond-N 9i Nomex 9s Kevlar 9j
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Abstract
A process for producing a substrate having a functionalised surface, which process comprises: (a) contacting the substrate with a carbene precursor, which carbene precursor is a compound of formula (III) or (IV) whose substituent groups are defined herein: (b) generating a carbene reactive intermediate from the carbene precursor so that it reacts with the substrate to functionalise the surface, thereby yielding an activated substrate; and (c) further functionalising the activated substrate obtained in (b). In (c), the activated substrate may be further functionalised by treating the activated substrate with a diazonium salt for the introduction of colour and/or another desired activity, and/or by treatment with hydrogen peroxide to produce a biocidal substrate. The invention further relates to carbene precursor compounds for use in the surface functionalisation process, and to processes for preparing certain precursor compounds.
Description
SURFACE FUNCTIONALISATION USING ARYLCARBENE REACTIVE INTERMEDIATES
The present invention relates to a process for the surface functionalisation of a substrate using an arylcarbene as the reactive intermediate. The invention in particular relates to a process for the surface functionalisation of a polymeric or an inorganic substrate for producing a substrate which is coloured and/or which has biocidal activity. The process of the invention can be used to produce biocidal substrates in which the biocidal activity is regenerable. The invention further relates to carbene precursor compounds for use in the surface functionalisation process, and to processes for preparing certain precursor compounds.
Dyestuffs which are used to colour natural and synthetic polymers, via covalent modification, most commonly rely upon the presence of highly reactive groups which have been coupled to the chromophoric species. Examples include the Procion and Gibacron range of dyes (which rely upon the reactivity of a chlorotriazinyl residue with nucleophilic residues on the substrate) and the Remazol range of dyes (which rely on a vinylsulfonyl residue as a nucleophilic acceptor for appropriate reactive groups on the polymer). Both of these reactive classes of dye have preferred substrates, which must possess the required nucleophilic characteristics. Development of this type of strategy still continues. However, in addition to the requirement for nucleophilic functionality on the substrate (usually hydroxyl or amino groups), this approach generally requires vigorous conditions, such as high temperature or strongly basic media, for bond formation to occur.
An alternative technique whereby highly reactive carbene or nitrene species are generated from inert precursors under less harsh conditions has also been investigated for application to dyeing and other surface modifying processes of various natural and synthetic polymers. The chemistry of carbenes and nitrenes is well documented, and these reactive entities are known to form covalent bonds with many types of functional group. The application of these species to the surface modification of organic solids using different approaches both for the generation of the required carbenes or nitrenes, and for their reaction with the solid surface has been reported.
French Patent No. 1 500 512 describes allowing carbenes to come into contact with an organic solid. The preferred method for surface modification is to allow the volatilised carbene to come into contact with the polymer. Inherent in this approach, however., are
limitations: only volatile (i.e. low molecular weight) carbenes, and those stable to relatively high temperatures, are applicable.
The application of carbenes generated from diazo compounds as suitable reactive dyes has been found to have important limitations, for example the ease of generation of the required diazo precursor (D.R. Braybrook et al., J. Photochem. Photobiol A: Chem, 1993, 70, 171) and the stability of the dye to the carbene generating process.
More recently, the use of carbenes carrying pendant activated side chains for colouration is described in WO 00/26180.
The rapid rise of Methicillin Resistant Staphylococcus Aureus ("Golden Staph") and other resistant bacteria has led to a significant increase in persistent bacterial infections in the human population world- wide. This has proved to be a major problem in surgery, particularly where post-operative recovery is complicated by infections acquired within the hospital environment. The problem of bacterial infection is not restricted solely to medical applications, though, and increasing pressure is being brought to bear on industry to control the spread of bacterial infection. Within the European Union, for instance, the European Biocidal Products Directive (98/9/EC) establishes a duty of care by European industries wherever possible to use antibacterial agents to protect the general population against harmful bacteria.
The potential of halogenated furanones and isothiazolones in antifouling polymers has been described in WO 99/01514. In this work, an organic antifouling agent was mixed with an extrudable polymer and examined for antifouling properties. However, limitations of this technology are that it relies on bulk dispersion of an expensive biocidal agent, it is restricted to a narrow range of polymers, and the polymer cannot be "reprimed".
These limitations have been addressed to some extent by covalent attachment of the furanone; for instance, the use of plasma activation with a small sample of polymer types is described in WO 96/01294, where biological efficacy is demonstrated.
Alternative strategies have been based on regenerable biocidal polymers. For example, US patent 5,490,983 relates to immobilised N-halainines for disinfectant use. The disinfectant properties of these compounds in solution has been known for many years, for instance in water purification and fabrics. The formulations of US patent 5,490,983 involve immobilisation of the biocidal agent in polymeric form, and the biocidal activity of the polymers is in principle regenerable. However, one intrinsic problem is that the regeneration technique requires chlorine. This is a toxic gas, and strenuous efforts to
minimise the use of this compound are being made across all industry. The technique of US patent 5,490,983 is therefore of inherently restricted applicability.
Limitations to existing technologies for controlling the growth and spread of bacteria and for introducing colour into a polymeric substrate, including those mentioned above, include the use of toxic reagents and lengthy synthetic procedures, the reliance on bulk dispersion of an expensive biocidal agent, restriction to a narrow range of polymers, that the biocidal activity cannot be "reprimed" since leakage of the biocidal agent leads to loss of activity, and a limited applicability in terms of the range and forms of polymeric substrate that can be functionalised. The present invention aims to address these issues.
Accordingly, the present invention provides a process for producing a substrate having a functionalised surface, which process comprises:
(a) contacting the substrate with a carbene precursor, which carbene precursor is selected from a compound of the following formula (III):
wherein
A is an aryl or heteroaryl ring; p is 1, 2, 3, 4 or 5;
R1 is selected from hydrogen, aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C1-C10 alkoxy which is substituted or unsubstituted, C1-C10 alkylamino which is substituted or unsubstituted, di(C1-C10)alkylamino which is substituted or unsubstituted, C1-C1O alkylthio which is substituted or unsubstituted, and C1- C10 alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R2), O or S wherein R2 is H or C1-C6 alkyl;
Q is selected from -N(Z1)(Z2) and -CH2-V-(W-R)n;
Z1 and Z2 are independently selected from aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C1-C10 alkoxy which is substituted or unsubstituted, Cj-C1O alkylamino which is substituted or unsubstituted,
d^CrCϊojalkylamino which is substituted or unsubstituted, C1-C10 alkylthio which is substituted or unsubstituted, and C]-C1O alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R2), O or S wherein R2 is H or C1-C6 alkyl;
V is -alk, -OaIk-, -alk-O- or -OaIk-O-, wherein alk is C1-C10 alkylene;
W is a functional group of one of the following formulae (a) to (c):
(a) X
— NH^ ^CH2
R is selected from H, C1-C6 alkyl which is unsubstituted or substituted, aryl which is unsubstituted or substituted and heteroaryl which is unsubstituted or substituted; and n is 1, 2 or 3;
and a compound of the following formula (IV):
wherein each of A and B, which are the same or different, is an aryl or heteroaryl ring; each of Q and Q2, which are the same or different, is -N(Z1)(Z2) or -CH2-V-(W-R)n, wherein Z1, Z2, V, W5 R and n are as defined above for formula (III); and each of p and r, which are the same or different, is 1, 2, 3, 4 or 5;
(b) generating a carbene reactive intermediate from the carbene precursor so that it reacts with the substrate to functionalise the surface, thereby yielding an activated substrate; and
(c) further functionalising the activated substrate obtained in (b).
In step (c) of the process of the invention as defined above the activated substrate obtained in step (b) is further functionalised. By "functionalise" as used in this context is meant the introduction of a, or of another, chemical functional group which exhibits desirable physical or chemical properties, by covalent attachment or by attachment through hydrogen bonding.
The activated substrate may be further functionalised by treating the activated substrate with a diazonium salt. Additionally or alternatively, the activated substrate may be further functionalised by treating the activated substrate with hydrogen peroxide, preferably aqueous hydrogen peroxide.
Typically, for the introduction of colour, step (c) involves treating the activated substrate obtained in step (b) with a diazonium salt, thereby forming a diazo coupled coloured substrate. The diazonium salt may be selected such that the desired colour is obtained, for instance cyan, magenta, yellow, black, red, green, blue or orange. Typically, the diazonium salt is of type ArN2 , wherein Ar is substituted or unsubstituted aryl or substituted or unsubstituted heteroaryl, wherein the aryl or heteroaryl ring may be substituted or unsubstituted at any or all positions of the Ar group. Typically Ar is substituted or unsubstituted phenyl. Examples include fast black K salt [2,5-dimethoxy-4- ((4-nitrophenyl)diazenyl)benzenediazonium chloride] or 4-Λ/,Λ/-dimethylaminobenzene diazonium chloride.
Alternatively, the activated substrate obtained in step (b) may be treated with a diazonium salt which is itself functionalised to confer a desired activity on the substrate. Thus, for the introduction of a particular desired activity, step (c) may involve treating the activated substrate in step (b) with a functionalised diazonium salt, the functionality of which confers the desired activity. The desired activity may be a particular hydrophobicity or hydrophilicity, for example.
For the introduction of biocidal activity, step (c) of the process as defined above involves treating the activated substrate obtained in step (b) with hydrogen peroxide, to yield a biocidal polymeric substrate.
The activated substrate obtained in step (b) may be treated with both a diazonium salt and hydrogen peroxide. Thus, for the introduction of colour and biocidal activity step
(c) of the process of the invention as defined above may comprise: (d) treating the activated substrate obtained in step (b) with a diazonium salt, thereby forming a diazo coupled coloured substrate, and (e) treating the diazo coupled coloured substrate obtained in step (d) with hydrogen peroxide.
Alternatively, for the introduction of biocidal activity and a further desired activity, step (c) of the process of the invention as defined above may comprise: (d') treating the activated substrate obtained in step (b) with a functionalised diazonium salt, whose functionality confers the desired activity, and (e') treating the diazo coupled substrate obtained in (c) with hydrogen peroxide.
Typically, when Q of formula (IV) is -N(Z1)(Z2), then Q2 is also -N(Z1)(Z2), wherein Z1 is as defined above and may be the same or different for Q and Q2, and wherein Z2 is as defined above and may be the same or different for Q and Q2.
Typically, when Q of formula (IV) is -CH2-V-(W-R)n, then Q2 is also -CH2-V-(W-R)n wherein V, W, R and n are as defined above and may be the same or different for Q and Q2.
Typically, Q of formula (III) is -N(Z1)(Z2), and Q and Q2 of formula (IV) are both -N(Z1)(Z2), wherein Z1 is as defined above and may be the same or different for Q and Q2, and wherein Z2 is as defined above and may be the same or different for Q and Q2.
Typically, Z1 is C1-C4 alkyl. Typically, Z2 is C1-C4 alkyl. Z1 and Z2 may be the same C1-C4 alkyl. Typically, p is 1. Typically, r is 1.
In one embodiment, Q of formula (III) and Q and Q2 of formula (IV) are both - CH2-V-(W-R)n wherein V, W, R and n are as defined above and may be the same or different for Q and Q2. Typically V is -OaIk-, wherein alk is C1-C10 alkylene. Typically W is a functional group of formula (c). Typically R is substituted or unsubstituted phenyl. Typically n is 1.
Typically A is phenyl. Typically B is phenyl.
In one embodiment, the present invention provides a process for producing a biocidal polymeric substrate, which process comprises:
(a) contacting the substrate with a diaryl carbene precursor, which precursor is selected from: a compound of the following formula (I):
,
wherein each of A and B, which are the same or different, is an aryl or heteroaryl ring; n is an integer of 1 to 3; y is 0 or an integer of 1 to 5; z is 0 or an integer of 1 to 5; provided that y and z are not both 0;
V is - alk, -O-alk-, -alk-O- or-O-alk-O-, wherein alk is C1-C10 alkylene;
W is a functional group of one of the following formulae (a) to (c):
(a) X
— NH' VCH2 —
(b) X
- CH2 7 VNH —
R is selected from H, C1-C6 alkyl which is unsubstituted or substituted, aryl which is unsubstituted or substituted and heteroaryl which is unsubstituted or substituted; and a compound of formula (II):
R is (CH2)mN(R3)(R4),
R3 is C1-C4 alkyl,
R4 is phenyl, m is an integer of 1 to 4; and
X is N2;
(b) generating a carbene reactive intermediate from the diarylcarbene precursor so that it reacts with the substrate to functionalise the surface, thereby yielding an activated substrate; and
(c) treating the activated substrate obtained in step (b) with aqueous hydrogen peroxide to yield a biocidal polymeric substrate. Typically in this embodiment the diaryl carbene precursor is a compound of formula (I). Typically y is an integer of 1 to 5 and z is an integer of 1 to 5.
The process of the invention offers significant economic and technical bulk advantages in that only the surface of the polymer is modified, and it is applicable to a diverse range of substrates including but not limited to natural and synthetic polymers and inorganic solids. The colouration of the diazo-coupled coloured substrates does not suffer from leaching, and the biocidal activity of the biocidal substrates is regenerable, that is, the polymer can be reactivated multiple times by treatment with a hydroperoxide solution.
The basic technique for functionalising the surface of a substrate using diarylcarbene precursors is described in WO 00/26180. That disclosure is concerned primarily with the surface functionalisation of a variety of polymeric substrates in order to colour the substrate.
The process of the present invention converts otherwise inert substrates, such as polymers, into activated substrates which possess surface functionality that allows the binding of a biocidal agent by addition of hydrogen peroxide or the addition of a functional group, such as a chromophoric group, by the addition of a diazonium salt. In the context of this invention, the biocidal activity is regenerable by subsequent retreatment of the activated substrate with fresh hydrogen peroxide. The fact that the functionality is confined to the surface of the substrate is advantageous because the bulk of the polymer is effectively sealed in and therefore the bulk properties of the polymer, including mechanical strength, remain unaffected by the biocidal, colour or other properties that are imparted to the surface.
The substrate treated in accordance with the present invention may be any natural or synthetic substrate which is capable of reaction with a carbene reactive intermediate generated from a diarylcarbene precursor of formula (III) or (IV) as defined above. The substrate is typically a natural or synthetic polymer including but not limited to cellulose, polyglycoside, polypeptide polyacrylates, polyacrylics, polyamides, polycarbonates, polyesters, polyethers, polyketones, polyolefms, rubbers, polystyrenics, polysulfones, polyurethanes, polyvinyls and their co-polymers; or an inorganic material including but not limited to silica, alumina, titania, glasses, and allotropes of carbon such as diamond, diamond-like carbon, graphite, fullerenes and nanotubes.
Where the substrate comprises a polymer, the molecular weight of the polymeric substrate may be selected according to the particular utility of the end product. For example, where the polymeric substrate treated in accordance with the present invention has biocidal properties, the molecular weight of the polymeric substrate may be selected according to the particular utility of the biocidal end product. In one embodiment, the substrate is selected from homopolymers and copolymers of ethylene or propylene, polyesters, polyamides, polystyrene, polytetrafluoroethylene, nylon, silica, cotton, silk and wool. In one embodiment of the invention the polymeric substrate is in a form that has a high surface area, for instance a powder or beads.
The polymer may be a homopolymer or a copolymer, for instance a block copolymer. It may thus be derived from monomeric units which are the same or different.
The polymer may be modified, for instance by admixture with an inorganic material. The substrate may thus comprise both a polymer and an inorganic material, for instance a mixture of a polymer and an inorganic material such as an inorganic filler. The substrate may, for example, comprise a mixture of one or more of the polymers referred to above and one or more of the inorganic materials referred to above. Modified polymers of this type are suitable for use in, for instance, semiconductor applications. Technical problems can arise when polymers are used as coating or bonding agents with semiconductors, owing to their different behaviour on heating. The inclusion of an inorganic filler with a polymer serves to modify the thermal properties of the polymer and make it better suited for use together with a semiconductor.
The diarylcarbene precursors of formula (I) are novel compounds. The present invention therefore further provides a compound of formula (I):
wherein each of A and B, which are the same or different, is an aryl or heteroaryl ring; n is an integer of 1 to 3; y is 0 or an integer of 1 to 5; z is 0 or an integer of 1 to 5; provided that z and y are not both 0;
V is - alk, -O-alk-, -alk-O- or -O-alk-O-, wherein alk is C1-C1O alkylene;
W is a functional group of one of the following formulae (a) to (c):
(a) X
Il C
— NH^ NCH2
(b) Il
C
— CH2' SNH
(C) X
Il
— NHκ NNH- wherein X is O, S or NH2 +; and
R is selected from H, C1-C6 alkyl which is unsubstituted or substituted, aryl which is unsubstituted or substituted, and heteroaryl which is unsubstituted or substituted; provided that the compound is not 4,4'-bis(iV-acetyl-2-ammoethyl)diphenyldiazomethane. Typically, z and y are not both 1.
In one embodiment of formula (I), y is an integer of 1 to 5 and z is an integer of 1
A C1-C1O alkyl group is an unsubstituted or substituted, straight or branched chain saturated hydrocarbon radical. Typically it is C1-C6 alkyl, for example methyl, ethyl, propyl, butyl, pentyl or hexyl, or C1-C4 alkyl, for example methyl, ethyl, i-propyl, n- propyl, t-butyl, s-butyl or n-butyl. When an alkyl group is substituted it typically bears one or more substituents selected from C1-C6 alkyl which is unsubstituted, aryl, cyano, amino, C1-C1O alkylamino, di(Ci-C10)alkylamino, amido, hydroxy, halo, carboxy, C1-Ce alkoxy, haloalkyl, sulfonic acid, sulfhydryl (i.e. thiol, -SH), C1-C1O thioether and sulfonyl. Examples of substituted alkyl groups include haloalkyl, hydroxyalkyl, aminoalkyl and alkoxyalkyl groups.
An aryl group is a substituted or unsubstituted, monocyclic or bicyclic aromatic group which typically contains from 6 to 14 carbon atoms, preferably from 6 to 10 carbon atoms in the ring portion. Examples include phenyl, naphthyl, indenyl and indanyl groups. An aryl group is unsubstituted or substituted. When an aryl group as defined above is substituted it typically bears one or more substituents selected from C1-C6 alkyl which is unsubstituted, aryl which is unsubstituted, cyano, amino, amido, hydroxy, halo, carboxy, C1-C6 alkoxy, haloalkyl, sulfonic acid and sulfonyl. Typically it carries 0, 1, 2 or 3 substituents.
An alkylene group is unsubstituted or substituted, straight or branched chain saturated divalent hydrocarbon group. Typically it is C1-C8 alkylene, for instance C1-C6 alkylene. Preferably it is C1-C4 alkylene, for example methylene, ethylene, i-propylene, n- propylene, t-butylene, s-butylene or n-butylene. It may also be pentylene, hexylene, heptylene, octylene and the various branched chain isomers thereof
A heteroaryl group is typically a 5- to 10 membered mono- or bicyclic heteroaromatic ring. It is generally a 5- or 6-membered ring, containing at least one heteroatom selected from O, S, N, P, Se and Si. It may contain, for example, 1, 2 or 3 heteroatoms. Examples of heteroaryl groups include pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, furanyl, thienyl, pyrazolidinyl, pyrrolyl, oxazolyl, oxadiazolyl, isoxazolyl, thiadiazolyl, thiazolyl, isothiazolyl, imidazolyl, pyrazolyl, quinolyl and isoquinolyl. A heteroaryl group may be unsubstituted or substituted, for instance, as specified above for alkyl. Typically it carries 0, 1, 2 or 3 substituents.
As used herein the term amino represents a group of formula -NH2. The term C1- C1O alkylamino represents a group of formula -NHR' wherein R' is a C1-C10 alkyl group, preferably a C1-C6 alkyl group, as defined previously. The term di(C1-C10)alkylamino
I l
represents a group of formula -NR 'R' r wherein W and R" are the same or different and represent C1-C10 alkyl groups, preferably C1-C6 alkyl groups, as defined previously.
A C1-C10 alkylthio group is a said C1-C10 alkyl group attached to a thio group.
A C1-C10 alkoxy group is a said C1-C10 alkyl group attached to an oxygen atom. A C1-C6 alkoxy group is a said C1-C6 alkyl group attached to an oxygen atom. A C1-C4 alkoxy group is a C1-C4 alkyl group attached to an oxygen atom. Examples of C1-C4 alkoxy groups include, -OMe (methoxy), -OEt (ethoxy), -O(nPr) (n-propoxy), -O(iPr) (isopropoxy), -0(nBu) (n-butoxy), -O(sBu) (sec-butoxy), -O(iBu) (isobutoxy), and -O(tBu) (tert-butoxy).
Alkylene and alkyl groups may be interrupted by one or more heteroatoms or heterogroups, such as S, O or N(R2) wherein R2 is H or C1-C6 alkyl. The phrase "optionally interrupted" as used herein thus refers to a C1-C10 alkyl group or an alkylene group, as defined above, which is uninterrupted or which is interrupted between adjacent carbon atoms by a heteroatom such as oxygen or sulfur, or a heterogroup such as N(R2) wherein R2 is H or C1-C6 alkyl.
For instance, a C1-C10 alkyl group such as n-butyl may be interrupted by the heterogroup N(R2) as follows: -CH2N(R2)CH2CH2CH3, -CH2CH2N(R2)CH2CH3, or -CH2CH2CH2N(R2)CH3. Similarly, an alkylene group such as n-butylene may be interrupted by the heterogroup N(R2) as follows: -CH2N(R2)CH2CH2CH2-, -CH2CH2N(R2)CH2CH2-, or -CH2CH2CH2N(R2)CH2-.
It is believed that hydrogen bonding is involved in the binding of the substituents Q and/or Q2 to peroxide. The reactivity of the diazo compound and its derived carbene could be modified by including electron releasing or electron withdrawing groups on the aromatic rings.
In formula (III) the or each group Q may occupy any available position on ring A. In formula (IV) the or each group Q and the or each group Q2 may occupy any available position on rings A and B respectively. When parameter "p" is 1, A is mono-substituted at any ring position by Q; for instance, when A is a phenyl group it may be substituted at any of positions 2, 3, 4, 5 and 6. When parameter "p" is 2, A is di-substituted at any two positions by Q; for instance, when A is a phenyl group it may be 2,3- 2,4-, 2,5-, 2,6-, 3,4- or 3,5-disubstituted. When parameter "p" is 3, A is tri-substituted at any three positions by Q; for instance, when A is a phenyl group it may be 2,3,4-, 2,4,5- or 3,4,5- tri-substituted. Likewise, when parameter "r" is 1, B is mono-substituted at any ring position by Q2; for instance, when B is a phenyl group it may be substituted at any of positions 2, 3, 4, 5 and
6. When parameter "r" is 2, B is di-substituted at any two positions by Q2; for instance, when B is a phenyl group it may be 2,3- 2,4-, 2,5-, 2,6-, 3,4- or 3,5-disubstituted. When parameter "r" is 3, B is tri-substituted at any three positions by Q ; for instance, when B is a phenyl group it may be 2,3,4-, 2,4,5- or 3,4,5- tri-substituted.
In formula (I) the or each chain -[CH2-V-(W-R)n] may occupy any available position on rings A and B. When parameter "y" is 1, B is mono-substituted at any ring position by -[CH2-V-(W-R)n]; for instance, when B is a phenyl group it may be substituted at any of positions 2, 3, 4, 5 and 6. When parameter "y" is 2, B is di-substituted at any two positions by -[CH2-V-(W-R)n] for instance, when B is a phenyl group it may be 2,3- 2,4-, 2,5-, 2,6-, 3,4- or 3,5-disubstituted. When parameter "y" is 3, B is tri-substituted at any three positions by -[CH2-V-(W-R)n]; for instance, when B is a phenyl group it may be 2,3,4-, 2,4,5- or 3,4,5- tri-substituted.
Likewise, when parameter "z" is 1, A is mono-substituted at any ring position by -[CH2-V-(W-R)n]; for instance, when A is a phenyl group it may be substituted at any of positions 2, 3, 4, 5 and 6. When parameter "z" is 2, A is di-substituted at any two positions by -[CH2-V-(W-R)n]; for instance, when A is a phenyl group it may be 2,3- 2,4-, 2,5-, 2,6- , 3,4- or 3,5-disubstituted. When parameter "z" is 3, A is tri-substituted at any three positions by -[CH2-V-(W-R)n]; for instance, when A is a phenyl group it may be 2,3,4-, 2,4,5- or 3,4,5- tri-substituted.
Parameter "n" qualifies the moiety represented by -W-R and thus defines the number of hydrogen-bonding groups W that are present in the compounds of formulae (I), (Ia), (III) and (IV). When n is 2 or 3, the moiety -V-(W-R)n in the chain -[CH2-V-(W- R)n] may be represented, for instance, as -(CR' 2)P-CR' (W-R)2 , -0-(CR' 2)P-CR' (W-R)2 , - (CR'2)p-C(W-R)3 or -O-(CR'2)p-C(W-R)3 wherein p is 0 or an integer of 1 to 10 and each R' is, independently, H or C1-C6 alkyl.
In one embodiment, the compound of formula (I) or formula (III) is of the following formula (Ia):
wherein each of y, V, W and R is as defined above.
In another embodiment, the compound of formula (I) or formula (III) is of the following formula (Ib):
wherein each of V5 W and R is as defined above.
A preferred example of a carbene precursor compound of formula (III) or (IV) is 1- {2-[4-(diazo-phenyl-methyl)-benzyloxy]-ethyl}-3-phenyl urea, hereafter compound XIII. This compound may be produced as shown in the following scheme 1. 4- Bromomethylbenzophenone, which can be prepared according to D. D. Tanner et ah, J. Org. Chem., 1980, 45, 5177, is first coupled with ethanolamine. The resulting benzophenone may be converted to the urea compound by treatment with phenyl isocyanate in dry dichloromethane. The urea compound may be converted to the hydrazone by treatment with hydrazine hydrate in refluxing methanol overnight, followed by removal of the solvent and extraction into dichloromethane. Oxidation of the hydrazone to the corresponding diazo diarylcarbene precursor may conveniently be performed using mercuric oxide in ether, or any other suitable oxidant.
Scheme 1
Carbene precursor compounds of formulae (III) or (IV) in which Q and/or Q2 is - CH2- V-(W-R)n, as defined above, may be produced by analogy with the method shown in scheme 1, from starting materials which are commercially available or which may readily be synthesised by known techniques.
Diarylcarbene precursors of formula (II) are known from WO 00/26180. A preferred example of a compound of formula (II) is 4-([iV-ethyl-iV-phenyl-2- . aminoethyl]oxymethyl)phenyl phenyl diazomethane, hereafter compound XVI. This compound may be produced as shown in the following scheme 2:
Scheme 2
A preferred example of a compound of formula (III) or (IV) is bis-4,4'-7\ζiV- dimethylamino diphenyldiazomethane, hereafter compound 3. This compound may be produced as shown in the following Scheme 3. Michler's ketone (1) can be converted to the known hydrazone (2) by reaction with hydrazine hydrate in ethanol. This hydrazone can be oxidised to diazomethane (3) using yellow mercuric oxide in tetrahydrofuran.
Scheme 3
Carbene precursor compounds of formulae (III) or (IV) in which Q and/or Q2 is -N(Z1)(Z2), as defined above, may be produced by analogy with the method shown in scheme 3, from starting materials which are commercially available or which may readily be synthesised by known techniques.
The method shown in Scheme 3 includes a new procedure for the preparation of the key intermediate 2. This procedure for preparing compound 2 represents a substantial simplification and improvement over the literature protocol (S. Hϋnig et al., Eur. J. Org. Chem, 2002, 10, 1603-1613).
Accordingly, the present invention further provides a process for producing a compound of formula (VII) or (VIII):
wherein A is an aryl or heteroaryl ring;
B is an aryl or heteroaryl ring; p is 1, 2, 3, 4 or 5; r is 1, 2, 3, 4 or 5;
R1 is selected from hydrogen, aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C1-C10 alkoxy which is substituted or unsubstituted, C1-Cj0 alkylamino which is substituted or unsubstituted, di(C1-Cio)alkylamino which is substituted or unsubstituted, Ci-C10 alkylthio which is substituted or unsubstituted, and C1- C10 alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R2), O or S wherein R2 is H or C1-C6 alkyl;
Q2, which may be the same as or different from Q, is -N(Z1)(Z2);
Z1 and Z2 are independently selected from aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C1-C10 alkoxy which is substituted or unsubstituted, C1-Ci0 alkylamino which is substituted or unsubstituted, di(Ci-Cio)alkylamino which is substituted or unsubstituted, C1-C1O alkylthio which is substituted or unsubstituted, and C1-C10 alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R ), O or S wherein R is H or C1-C6 alkyl; the process comprising treating a compound of formula (V) or (VI) with hydrazine in the presence of heat and a solvent:
Typically, hydrazine is used in the form of hydrazine hydrate. Any suitable solvent is employed, for instance a polar protic solvent such as an alcohol. Typically, the solvent is ethanol. The reaction is carried out with heating, typically at the reflux temperature of the solvent used. For example, when the solvent is ethanol the reaction is suitably carried out at a temperature of 78 °C or higher, e.g. at a temperature of 80 °C.
The resulting compounds of formulae (VII) and (VIII) may subsequently be converted into carbene precursor compounds of formulae (III) and (IV), respectively, as defined above. Accordingly, in one embodiment, the process as defined above further comprises oxidising a compound of formula (VII) or (VIII) to produce a carbene precursor compound of formula (III) or (IV) respectively:
Any suitable oxidant may be used. Typically, the oxidant is mercuric oxide in an aprotic polar solvent, for instance tetrahydrofuran (THF) or an ether. More typically, this oxidation is conducted in the presence of a base, for instance a metal hydroxide and sodium sulphate. The metal hydroxide is typically an alkali metal hydroxide, for instance potassium hydroxide. A saturated solution of the metal hydroxide is generally used. The solvent used for the metal hydroxide is suitably a polar protic solvent such as an alcohol, for instance ethanol. The solvent used for the solution of the compound of formula (VII) or (VIII) is suitably a polar aprotic solvent, for instance tetrahydrofuran.
The carbene precursor compound of formula (III) or (IV) thus produced may be employed as the carbene precursor in step (a) of the process of the invention for producing a substrate having a functionalised surface, as defined above.
By "carbene reactive intermediate", as used herein, is meant a reactive species comprising a formally divalent carbon atom. The carbene reactive intermediate is generated from the carbene precursor of formula (III) or (IV) by treatment under conditions which result in an irreversible covalent reaction with the substrate. Generally the carbene is generated by heating or irradiating the substrate and pre-adsorbed carbene precursor compound; since the carbene precursor of formula (II) or (IV) is usually intensely coloured (red-orange), the formation and insertion of the carbene is most conveniently monitored by decolourisation.
The coloured substrates produced by the process of the present invention may be used in the dye industry for the preparation of non-leaching dyed polymers, such an example would be in food and drink packaging; they may be used to confer specific wavelength absorbing properties such as ultraviolet or infrared absorption; the ability of the substrates to show photochromism could be utilised in the medical market for contact lenses; the introduction of fluorescent markers allows security tagging applications. The coloured substrates produced by the present invention are capable of being irreversibly
dyed with little or no leaching from the surface, which is an important practical advantage associated with their use.
The biocidal polymeric substrates produced by the process of the present invention may be used in many different fields, but they particularly have utility in any context where the occurrence of bacterial, viral or fungal infection is a risk. For instance, they may have medical, industrial, catering, domestic, public health or military applications. Hydrogen peroxide-based biocidal agents are tasteless and odourless and are therefore preferable in many contexts to chlorine-based biocides.
The biocidal polymeric substrates are effective against a wide range of microorganisms including Gram-positive and Gram-negative bacteria, as well as viruses. Specific examples of bacteria include S. Aureus and E. CoIi.
Specific examples of the downstream uses of biocidal polymeric substrates produced by the process of the present invention include use in water purification systems, both small-scale and industrial; use in water filtration systems, for instance water filter cartridges for domestic and military use, in emergency applications and disaster relief; use in air purification systems, for instance air recirculation systems in aircraft, breathing apparatus and environmental protection products; use in medical and military equipment and clothing such as surgical gowns, surgical masks and surgical instruments; and use in medical devices such as implants, stents and catheters.
The biocidal polymeric substrates produced by the process of the present invention are capable of being regenerated on multiple occasions after use by treatment with hydrogen peroxide, which is an important practical advantage associated with their use. Regeration may be achieved by simple re- washing of the substrate with aqueous hydrogen peroxide.
The process is extendable from chromophoric and biocidal applications to include other chemical and reactive functionality. The process may be used to produce a substrate having a surface of a particular hydrophobicity or hydrophilicity, for example.
The present invention is further illustrated in the Examples which follow:
Example 1: Preparation of l-{2-[4-(Diazo-phenvI-methvI)-benzyIoxy1-ethyI}-3- phenyl urea (XIII)
4-(Ammoethoxymethyl)benzophenone hydrochloride (X)
To a solution of dry ethanolamine (1.0 g, 16.4 mmol) in dry THF (5 ml) was added sodium hydride (0.7 g, 60% dispersion in mineral oil, 16.4 mmol) in several portions over a period of 5 minutes. A further aliquot of dry THF (5 ml) was added and the resulting mixture stirred for Ih. To this mixture was added a solution of 4-bromomethyl benzophenone (IX) (4.5 g, 16.4 mmol) in dry THF (25 ml). The resulting mixture was allowed to stir for 18h. before being concentrated in vacuo. The residue was partitioned between chloroform and water and the organic layer collected, washed with water then extracted with 2M HCl. The combined acidic extractions were washed once with chloroform before being concentrated in vacuo. The residue was dissolved in a 9:1 chloroform:methanol mixture and dried over sodium sulfate before being concentrated in vacuo to yield (X) (2.6 g, 55%) as a pale yellow solid which was used without further purification.
l-[4-Benzoyl-benzyloxyethyl] -3 -phenyl urea (XI)
To a stirred suspension of (X) (3.49 g, 12 mmol) in dry DCM (10 ml) was added dry triethylamine (10 ml). After 0.25h. stirring a solution of phenyl isocyanate (2.14 g, 18 mmol) in dry DCM (10 ml) was added in one portion. The mixture was stirred for a further 16 h. then quenched with water (10 ml). The organic layer was collected and washed ' sequentially with water, 2M HCl, and water. The organic layer was dried over MgSO4 and purified by flash chromatography on silica gel eluting with 2:1 petrol 40/60:ethyl acetate (Rf= 0.1) to yield (XI) (3.01 g, 67 %) as a white solid; m.p. 129-1320C; δH (DMSO-d, 200 MHz) 3.39 (q, 2H, J= 5.1 Hz, NHCH2), 3.58 (t, 2Η, J= 5.0 Hz, OGH2CH2), 4.65 (s, 2H, OCiβAr), 6.32 (t, IH, J= 5.2 Hz CH2N#C(O)NHAr), 6.90 (t, IH, J= 7.3 Hz, 4"-H), 7.24 (t, 2H, J= 7.4 Hz, 3"-, 5"-H), 7.44 (d, 2H, J= 7.6 Hz, T-, 6"-H), 7.56 (m, 4H, 3-, 5-, 3'-, 5'-H), 7.70 (m, 5H, 2-, 4-, 6-, T-, 6"-H) 8.63 (s, IH, CH2NHC(O)N#Ar) ppm; δC (DMSO-d, 400 MHz) 39.9 (CH2CH2NH), 70.4 (CH2CH2O), 72.1 (ArCH2O), 118.4 (T-, 6"-C), 121.9 (4"-Q, 128.1 (2-, 6-, 2'-, 61- C), 129.4 (3"-, 5"-C), 130.4, 130.6 (3-, 5-, 3'-, 5'- Q, 133.5 (4'-0, 136.9, 138.0 (1-, V-C), 141.3 (4-Q, 144.4 (1"-Q, 156.1 (N(H)C(O)NH), 196.3 (Ar2C(O)) ppm; υmax 3431 (N-Hstr), 1724 ((R(H)N)2C=Ostr), 1658 (Ar2C=0Str)
cm"1; m/z 375 [MfH]+ (5%), 397 [M+Na]+ (10%), 433 [M+MeCN+NH4]+ (100%); found 375.1703, C23H23N2O3 requires 375.1709.
l-{2-r4-(Hvdrazono-phenyl-methyl)-benzyloxy]-ethyl}-3-phenyl urea (XII)
A solution of (XI) (0.7 g, 1.9 mmol) in methanol (10 ml) was treated with hydrazine hydrate (1.9 g, 38 mmol). The resulting solution was heated to a gentle reflux for 16h., cooled and concentrated in vacuo. The residue was partitioned between DCM and water and the organic layer was collected, washed with water and dried over MgS 04. Concentration in vacuo yielded (XII) (0.7 g, 99 %) as a thick cloudy white oil; δH (CDC13, 200 MHz) 3.47 (m, 2H, N(H)CH2CH2), 3.61 (m, 2H, OCH2CH2), 4.43, 4.52 (s, 2H, ArCH2θ), 5.49 (s, 2H, NNH2), 6.55 (m, IH, CH2NHC(O)), 6.99 (m, IH, 4"-H), 7.22 (m, 7H, 3-, 3'-, 3"-, 4'-, 5-, 5'-, 5"-H), 7.46 (m, 6Η, 2-, 2'-, 2"-, 6-, 6'-, 6"-H), 7.80 (m, 1Η, ArNHC(O)) ppm; υmax 3435 (N-Ηstr), 1734 ((R(Η)N)2C=Ostr) cm"1; m/z 389
[M+H]+ (15%), 447 [M+MeCN+NH4]+ (100%); found 389.1973, C23H25N4O2 requires 389.1978.
l-{2-|"4-(Diazo-phenyl-methyl)-benzyloxyl-ethyl>-3-phenyl urea (XIII)
To a vigorously stirred mixture of yellow mercury oxide (0.18 g, 0.8 mmol), sodium sulfate (0.14 g, 1 mmol) and sat. KOH in ethanol (1 ml) was added a solution of (XII) (0.27 g, 0.7 mmol) in THF (10 ml). The mixture was stirred for 16h. in the dark, then filtered through a pad of celite. The filtrate was collected and concentrated in vacuo to yield (XIII) (0.27 g ,100 %) as a dark red solid; m.p. 111-115 0C, decolurises at 1360C; δ H (CDC13, 200 MHz) 3.43 (m, 2H, CH2CH2NH), 3.69 (m, 2H, CH2CH2O), 4.49 (s, 2Η, ArCH2O), 5.80 (bs, 1Η, CΗ2NHC(O)), 7.02 (m, 1Η, 4"-H), 7.41-7.44 (m, 13Η, Ar-H), 7.44 (s, 1Η, ArNHC(O)) ppm; δC (CDC13, 400 MHz) 40.3 (CH2NH), 62.4 (CH2CH2O), 68.0 (ArCH2O), 120.3 (4"-Q, 123.2 (4'-Q, 125.0, 125.8, 126.5, 126.7 (2-, T-, 2"-, 6-, 6'-, 6"-Q, 128.9, 129.1, 129.2, 129.3 (1-, 1'-, 3-, 3'-, 3"-, 5-, 5'-, 5"-Q, 135.0, 138.9 (4-, 1 "-C), 156.5 (NHC(O)NH) ppm; Umax 3344 (N-Hstr), 2042 (R2C=N=N), 1658 ((R(H)N) 2C=0str) cm"1; m/z 445 [M+MeCN+NH4]+ (100%).
Reference Example 1: Preparation of 4-(W-Ethyl-iV-phenyl-2- aminoethylioxymethyDphenyl phenyl diazomethane (XVI)
4-(|"JV-Ethyl-N-phenyl-2-aminoethyl]oxymethyl) benzophenone (XIV)
2-(iV-Ethylanilino)ethanol (3.03 g, 18.4 mmol, 1.2 eq) in THF (20 cm3) was treated with NaH (60% dispersion in oil, 524 mg, 13,1 mmol, 1.4 eq) and stirred at 20°C for 1 hour. 4-Bromomethylbenzophenone (IX) (4.21 g, 15.3 mmol) was then added and stirring continued for 72 hours. Excess solvent was removed in vacuo and the residue diluted with DCM, washed with water and NaHCO3 solution (sat.), dried (MgSO4) and solvent removed under vacuum. The resulting oil was purified by flash chromatography, eluting with petroleum (bp 40-60°C):EtOAc (9:1), to give the desired product (XIV) as a yellow oil (4.36 g, 80%), Rf = 0.54 (4:1, petrol:EtOAc) (Found: C, 78.34; H, 6.86; N, 5.29. C24H25NO2 requires C, 80.19; H, 7.01; N, 3.90%); υmax (film/cm"1 1658 (s), 1598 (s), 1506 (s); δH (200 MHz; CDCl3) 1.22 (3 H, t, J7, CH3), 3.49 (2 H, q, J7, CH2CH3), 3.58-3.79 (4 H, m, OCH2CH2N), 4.66 (2 H, s, ArCH2O), 6.69-6.79 (3 H, m, ArH o- and;?- to NR2), 7.28 (2 H, dd, J 7, 7, ArH m- to NR2), 7.46-7.68 (5 H, m, ArH), 7.80-7.88 (4 H, m, ArH o- to C=O); δc (50.3 MHz; CDCl3) 12.2 (CH3), 45.5 (NCH2CH3), 50.1 (NCH2CH2O), 68.5 (NCH2CH2O), 72.2 (Ai-CH2O), 111.8 (ArCH o- to NR2), 115.8 (ArCH p- to NR2), 127.0, 128.3, 129.3, 130.0 and 130.3 (ArCH o- and m- to C=O and ArCH m- to NR2), 132.4 (AxCHp- to C=O), 136.8 and 137.7 (4° ArCC=O), 143.2 (4° ArCCH2O), 147.7 (4° ArCNR2), 196.4 (C=O); m/z (APCI+) 360 ([M + H]+, 30%); HRMS C24H26O2N requires 360.1963; found 360.1963.
4-(riV-Ethyl-Ar-phenyl-2-aminoethyl]oxymethvD benzophenone hvdrazone (XV)
The above benzophenone (701 mg, 1.95 mmol) was reacted with hydrazine hydrate yielding the hydrazone (XV) as a colourless oil (710 mg, 97%),υmax (filmycm"1 1598 (s), 1506 (s); δH (500 MHz; CDCl3) 1.27 and 1.31 (3 H, 2* t, J7, CH3), 3.52 and 3.57 (2 H, 2* q, J7, CH2CH3), 3.60-3.87 (4 H, m, NCH2CH2O), 4.63 and 4.71 (2 H, 2* s, ArCH2O), 5.57 (2 H, br s, NNH2), 6.76-6.87 (3 H, m, ArH o- and p- to NR2), 7.30-7.42 (7 H, m, ArH), 7.53-7.64 (4 H, m, ArH o- to C=N); δc (125.8 MHz; CDCl3) 12.0 (CH3), 45.1 and 45.2 (NCH2CH3), 49.8 (NCH2CH2O), 67.8 and 68.1 (OCH2CH2N), 72.6 and 72.7 (Ai-CH2O), 111.5 and 111.6 (Ai-CH o- to NR2), 115.5 and 115.6 (ArCHp- to NR2), 126.2, 127.1, 127.7, 127.8, 128.1, 128.5, 128.6, 128.9, 129.0 and 129.1 (ArCH), 131.9 and 132.8 (4°
ArCCH2O), 137.7, 137.8, 138.3 and 138.9 (4° ArCC=N), 147.5 (4° ArCNR2), 148.1 (C=NNH2); m/z (APCI+) 374 ([M + H]+, 5%), 357 (5%), 209 (100%).
4-([iV-Ethyl-N-phenyl-2-aminoethyl1oxymethvDphenyl phenyl diazomethane (XVD The above benzophenone hydrazone (701 mg, 1.88 mmol) was reacted with mercuric oxide and sodium sulphate yielding the diazomethane (XVI) as a purple liquid (690 mg, 99%) (Found: C, 78.06; H, 6.89; N, 12.36. C24H25N3O requires C, 77.60; H, 6.78; N, 11.31%); υmax (filmycrn 1 2037 (s), 1559 (m); δH (500 MHz; CDCl3) 1.26-1.32 (3 H, m, CH3), 3.50-3.80 (6 H, m, NCH2CH3 and NCH2CH2O), 4.65 (2 H, s, ArCH2O), 6.78-6.86 (3 H, m ArH o- and p- to NR2), 7.28-7.65 (11 H, m ArH); δc (125.8 MHz; CDCl3) 12.1 (CH3), 45.3 (NCH2CH3), 50.0 (NCH2CH2O), 67.9 (OCH2CH2N), 72.8 (ArCH2O), 111.7 (ArCH o- to NR2), 115.6 (ArCH/?- to NR2), 125.0, 125.5, 126.3, 128.3, 128.4, 128.7, 129.0, 129.1 and 129.2 (4° ArC and Ai-CH), 135.7 (4° ArCCH2O), 147.7 (4° ArCNR2); m/z (APCI+) 344 ([M - N2J+, 20%), 209 (100).
Example 2: Preparation of functionalised polymer beads/powder.
To a solution in dry THF of compound (XIII) described in Example 1 , or compound (XVI) described in Reference Example 1 , was added the required polymer and the mixture concentrated in vacuo. The resulting dark red stained polymer was collected and heated using a heat gun until the dark red coloration had disappeared. The resulting solid was placed in the extraction thimble and Soxhlet extracted with acetone for 12 h. The solid was collected and dried to yield the functionalised polymer.
a Weight-weight Ratio of compound 1 or 2 to polymer used during functionalisation step; b Amberlite XAD- 4 non ionic absorbant (Aldrich 21,648-8); c Ultra high molecular weight polyethylene powder (Aldrich 43,426-4 d Polytetrafluoroethylene beads (Aldrich 18,247-8) e (Sorbsil™ C60H(40-60 μM) silica gel).
Example 3: Hydrogen peroxide loading on functionalised polymers
Method for hydrogen peroxide loading
A sample of the required polymer was suspended in 50% aqueous hydrogen peroxide for 1 hour and the polymer was collected by filtration and washed with 1-1.51 of water, collected and treated with a solution of 10% potassium iodide in aqueous acetic acid. After standing for 5 minutes, a 1% aqueous starch solution was added, and the suspension left to stand for 1 hr. The resulting dark blue colour solution was titrated with sodium thiosulfate until the colourless endpoint. The mixture was left for a further hour and any further blue coloration of the solution was further titrated with sodium thiosulfate.
N/D = Not detected; + = blue colour
Example 4: Stability of hydrogen peroxide functionalised polymer
Method for the stability testing of hydrogen peroxide functionalised polymer
A sample of the functionalised polymer B (50mg), described in Example 3, was suspended in 50% aqueous hydrogen peroxide for 18 hr. The polymer was collected by filtration and washed with 1-1.51 of water, collected in a stoppered vial and stored at room temperature in a darkened cupboard. At regular time intervals 10 mg samples were collected and tested for the hydrogen peroxide loading using the above procedure.
Table 3 Stability of hydrogen peroxide functionalised polymer B.
Method for the regenerability testing of hydrogen peroxide functionalised polymer
A sample of the required polymer (0.05g) was suspended in 50% aqueous hydrogen peroxide (2ml) for 18 h. The polymer was collected by filtration and washed with 1-1.51 of water, collected and treated with solution of 10% potassium iodide in acetic acid and water. After standing for 5 minutes a 1% aqueous starch solution was added and the suspension left to stand for Ih. A positive test for hydrogen peroxide was the formation of a dark blue coloured solution. This mixture was then quenched with sodium thiosulfate until colourless and the polymer filtered and washed with 1-1.51 of water. The polymer was then re-suspended in fresh 50% aqueous hydrogen peroxide (2ml) and the cycle repeated.
The above procedure was carried out using polymer samples B and F described in Example 3. The results are shown in the following Table 4:
Table 4 Re enerabilit of h dro en eroxide functionalised polymers B and F
+ = blue colour.
Example 6: Bioassay
Method for Bioassay
An agar plate inoculated with the chosen organism was punched with 10mm wells. To these wells was added 20μl of molten agar. When set the polymer sample was added to
the well so that a thin layer of polymer was produced. The well was then sealed with a further 50μl of molten agar, the plate incubated overnight at 37°C and the diameter of the inhibition zones measured.
The results obtained with two different microorganism strains are set out in the following Tables 5 and 6.
(a) S Aureus
Table 5: Zones of inhibition results against S. Aureus using 10 mm wells in Hole-Plate
Bioassay (against Ceph C standard)
N/D = Not detected
Table 6: Zone of inhibition results against E. CoIi using 10 mm wells in Hole- Plate Bioassay (against Cevh C standard)
N/D - Not detected.
Example 7: Preparation of bis-4,4'-iV,iV-dimethylamino diphenyldiazomethane (3)
Bis-4,4'-λζN-dimethylamino diphenyldiazomethane (3) was prepared and polymers were functionalised as shown in Scheme 4.
Scheme 4
4,4-Bis-iVyV-dimethylamino benzophenone hydrazone (2)
A suspension of Michler's ketone (5.0 g, 19 mmol) in ethanol (10 ml) was treated with hydrazine hydrate (5.0 ml, 100 mmol). The resulting mixture was heated to a gentle reflux for 72h., cooled and concentrated in vacuo. The residue was diluted with 2-propanol (50 ml) and the resulting solid collected by filtration and dried in vacuo to yield 2 (4.8 g, 91 %) as a beige solid; m.p. 151-1520C; δπ (d6-DMSO, 200 MHz) 2.89 (s, 2H, 2XNCH3), 2.97 (s,
2H, 2xNCH3), 5.81 (br s, 2H, NNH2), 6.64 (d, 2H5 J= 8.9 Hz, 3-, 5-H), 6.86 (d, 2Η, J= 8.8 Hz5 V-, 5'-H), 7.05 (d, 2Η, J= 8.8 Hz, T-, 6'-H)5 7.22 (d, 2Η, J= 8.9 Hz, 2-, 6-H) ppm; δc (d6-DMSO5 200 MHz) 40.9 (4x NCH3), 112.6 (3-, 5-Q, 113.2 (3'-, 5'-C), 121.1 (1-Q, 127.8 (2-, 6-Q, 128.7 (l'-Q, 130.3 (2'-, &-Q, 150.6 (Ar2C=NNH2) ppm; υmax 1626, 1545, 1350, 1180 cm"!; ms 283 ([M+H]+, 100%), 305 ([M+Na]+, 10%).
Bis-4,4'-i\yV-DimethylaniIine diazomethane (3)
To a vigrously stirred mixture of yellow mercury oxide (0.46 g, 2.1 mmol), sodium sulfate (0.35 g, 2.5 mmol) and sat. potassium hydroxide in ethanol (3 ml) was added a solution of 2 (0.5 g, 1.8 mmol) in tetrahydrofuran (10 ml). The mixture was stirred for 18h. in the dark, then filtered through a pad of celite. The filtrate was collected and concentrated in vacuo to yield 3 (0.5 g ,100 %) as a dark green solid; m.p. 94 0C (dec); δH (CDCl3, 200 MHz) 2.96 (s, 12H5 4xNCH3), 6.81 (d5 4Η, J= 6.8 Hz5 3-, 3'-, 5-, 5'-H), 7.17 (d, 4H5 J= 6.8 Hz5 2-, T-, 6-, 6'-H) ppm; δc (CDCl35 200 MHz) 149.1 (4-, 4'-C), 132.7 (l-5 V-Q, 126.8 (3-, 3'-, 5-, 5'-Q, 114.0 (2-, T-, 6-, 6'-Q5 41.1 (4xNCH3) ppm; υmax 2020
(Ar2C=N+=N"), 1606, 1519, 1356 cm"1.
Example 8: Functionalisation of polymer beads / powder / fabric (4a-q)
To a solution of 3 in either toluene or tetrahydrofuran (10 ml) (Table 1) was added the required polymer and the mixture concentrated in vacuo. The polymer was collected and heated in vacuo to HO0C for 15 minutes. The resulting solid was Soxhlet extracted with acetone for 6h and the solid collected and dried to yield the functionalised polymer 4a-q (Table 7).
Table 7: Functionalised polymers prepared.
Table 7 a Ratio of diazomethane to polymer used during functionalisation step. b Solvent used for diazo absorption onto the polymer. c Amberlite XAD-4. d Cross Linked Polystyrene. e Ultra High Molecular Weight Polyethylene. f Surface Modified Ultra High Molecular Weight Polyethylene. g Polyethylene. h Polypropylene. ' Nylon-610. j Normal Knitted Cotton. k Mercerised Woven Cotton. ' Poly(ethylene terephthalate).
Example 9: Dyeing of polymers
(i) Fast Black dyed polymers 6a-6r
Polymers 4a-r were dyed by immersing the functionalised polymer in a 0.1 M solution of fast black K salt 5 in acetone for 18h. The polymer was then collected by filtration and washed with acetone to furnish the corresponding diazo polymers 6a-r (Table 2, Scheme 5).
p ) = XAD-4 6a Tencel 6k ^ PSXL-S 6b Viscose 61 UHMWPE 6c NK-Cotton 6m SMUHMWPE 6d MW-Cotton 6n PE-S 6e Cotton Wool 6o PP 6f PET 6p PP-S 6g Silica Gel 6q N-610 6h Alumina 6r Hybond-N 6i Kevlar 6j
Scheme 5
(ii) 4-N^V-Dimethylamino benzene diazonium dyed polymer
Preparation of 4-iV^V-Dimethylamino benzene diazonium chloride (7)
To an ice cold solution of JVjN-Dimethyl-p-phenylene diamine (1.0 g, 7.3 mmol) in ethanol (10 ml) was added concentrated hydrochloric acid (0.72 ml, 7.3 mmol) and isoamyl nitrite (1.6 ml, 12 mmol). The mixture was stirred for 10 minutes, than diluted with diethyl ether (50 ml). The resulting solid was filtered and washed with diethyl ether. Drying in vacuo yielded 6 (l.lg, 77%) as a dark green solid. δH (d6-DMSO, 200 MHz) 3.29 (s, 6H, 2XNCH3), 7.10 (d, 2Η, J= 7.8 Hz, 3-, 5-H), 8.30 (d, 2H, J= 7.8 Hz, 2-, 6-H) ppm; δc (d6- DMSO, 200 MHz) 157.0 (A-Q, 134.9 (2-, 6-Q, 114.8 (3-, 5-Q, 89.91 (1-Q, 41.4 (2xNCH3) ppm.
4-Λ^V-Dimethylamino benzene diazonium dyed UHMWPE (8)
UHMWPE 4c was immersed in a IM solution of 7 in methanol for 18h. The polymer was collected by filtration and washed with acetone, water and methanol to furnish the corresponding diazo polymer 8 (Table 8).
(pλ = UHMWPE 8
Scheme 6
Table 8 Dyed polymers prepared
To a flask containing hydrogen peroxide (50 % aqueous solution, 10 ml) was added the functionalised polymer (4a-f or 8) and the mixture allowed to stand at room temperature in the dark for 16h. The polymer was isolated by filtration and the solid washed with water (1-1.51) to yield the hydrogen peroxide adduct (Scheme 7).
Θ p) = XAD-4 9a Tencel 9k PSXL-S 9b Viscose 91 UHMWPE 9c NK-Cotton 9m SMUHMWPE 9d MW-Cotton 9n PE-S 9e Cotton Wool 9o PP 9f PET 9p PP-S 9g Silica Gel 9q N-610 9h Alumina 9r Hybond-N 9i Nomex 9s Kevlar 9j
Scheme 7
Method for determining oxidant loading.
A sample of the required polymer was suspended in 50% aqueous hydrogen peroxide for 18h. The polymer was filtered and washed with water, collected and treated with solution of 10% aqueous potassium iodide (0.1 M) in acetic acid. After standing for 5 minutes a 1% aqueous starch solution was added and the suspension left to stand for Ih. The resulting dark blue coloured solution was titrated with sodium thiosulfate until the colourless
endpoint. The mixture was left for an hour and any further blue coloration of the solution was further titrated with sodium thiosulfate until no further colour persisted (Table 9).
Table 9: Hydrogen peroxide loading on functionalised polymers.
N/D = Not detected.
Example 11: Bio testing.
Method for biotesting
An agar plate inoculated with the S. Aureus was punched with 10mm wells. To these wells was added 20μl of molten agar. When set the polymer sample was added to the well so that an even thin layer of polymer was produced. The well was then sealed with a further 50μl of molten agar, the plate incubated overnight at 370C and the zones measured after this (Table 10).
Bioassay results & Aureus.
Table 10 Zone of inhibition results against S. Aureus using 10mm wells.
N/D = Not detected.
Claims
1. A process for producing a substrate having a functionalised surface, which process comprises:
(a) contacting the substrate with a carbene precursor, which carbene precursor is selected from a compound of the following formula (III):
wherein
A is an aryl or heteroaryl ring; p is 1, 2, 3, 4 or 5;
R1 is selected from hydrogen, aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C1-C10 alkoxy which is substituted or unsubstituted, C1-C10 alkylamino which is substituted or unsubstituted, di(Ci -C^alkylamino which is substituted or unsubstituted, C1-C10 alkylthio which is substituted or unsubstituted, and C •l- C1O alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R2), O or S wherein R2 is H or C1-C6 alkyl;
Q is selected from -N(Z1)(Z2) and -CH2-V-(W-R)n;
Z1 and Z2 are independently selected from aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C1-C10 alkoxy which is substituted or unsubstituted, C1-C10 alkylamino which is substituted or unsubstituted, di(C1-C10)alkylamino which is substituted or unsubstituted, C1-C10 alkylthio which is substituted or unsubstituted, and C1-C10 alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R2), O or S wherein R2 is H or C1-C6 alkyl;
V is -alk, -O-alk-, -alk-O- or -OaIk-O-, wherein alk is C1-C10 alkylene;
W is a functional group of one of the following formulae (a) to (c): (a) X
Il
— NH' VCH2 —
(b) X
- CH2' NNH —
(C) X
C
— NH' XNH- wherein X is O, S or NH2 +; and
R is selected from H, C1-C6 alkyl which is unsubstituted or substituted, aryl which is unsubstituted or substituted and heteroaryl which is unsubstituted or substituted; and n is 1, 2 or 3;
and a compound of the following formula (IV):
wherein each of A and B, which are the same or different, is an aryl or heteroaryl ring; each of Q and Q2, which are the same or different, is -N(Z1)(Z2) or -CH2-V-(W-R)n, wherein Z1, Z2, V, W, R and n are as defined above for formula (III); and each of p and r, which are the same or different, is 1, 2, 3, 4 or 5;
(b) generating a carbene reactive intermediate from the carbene precursor so that it reacts with the substrate to functionalise the surface, thereby yielding an activated substrate; and
(c) further functionalising the activated substrate obtained in (b).
2. A process according to claim 1 wherein step (c) comprises treating the activated substrate obtained in step (b) with hydrogen peroxide, to yield a biocidal substrate.
3. A process according to claim 1 wherein step (c) comprises treating the activated substrate obtained in step (b) with a diazonium salt, thereby forming a diazo coupled coloured substrate.
4. A process according to claim 3 wherein the diazonium salt is ArNa+, wherein Ar is selected from aryl which is unsubstituted or substituted and heteroaryl which is unsubstituted or substituted.
5. A process according to claim 1 wherein step (c) comprises treating the activated substrate obtained in step (b) with a functionalised diazonium salt, thereby forming a diazo coupled substrate wherein the functionality of the diazonium salt confers a desired activity on the diazo coupled substrate.
6. A process according to any one of claims 3 to 5, which process further comprises: treating the diazo coupled substrate obtained in step (c) with hydrogen peroxide, to yield a biocidal diazo coupled substrate.
7. A process according to any one of the preceding claims wherein the substrate comprises a polymer.
8. A process according to any one of the preceding claims wherein the substrate comprises an inorganic material.
9. A process according to any one of the preceding claims wherein the substrate comprises a mixture of a polymer and an inorganic filler.
10. A process according to any one of claims 7 to 9 wherein the polymer is a homopolymer or a copolymer.
11. A process according to any one of claims 7 to 10 wherein the polymer is selected from polyolefins, polyesters, polyamides, polystyrenics, polytetrafluoroethylene, polyglycosides, polypeptides, polyacrylates, polyacrylics, polycarbonates, polyethers, polyketones, rubbers, polysulfones, polyurethanes, polyvinyls, cellulose and block copolymers.
12. A process according to any one of claims 8 to 11 wherein the inorganic material is selected from silica, alumina, titania, glass and an allotrope of carbon.
13. A process according to any one of the preceding claims wherein the carbene precursor is selected from bis-4,4'-ΛζiV-dimethylammo diphenyldiazomethane and l-{2-[4- (diazo-phenyl-methyl)-benzyloxy] -ethyl} -3 -phenyl urea.
14. A substrate which is obtainable by a process as defined in any one of the preceding claims.
15. A biocidal polymeric substrate which is obtainable by a process as defined in claim 2 or claim 6.
16. A water purification system, a water filtration system, an air purification system, an item of clothing, an item of surgical equipment or a medical device which incorporates a biocidal polymeric substrate as defined in claim 15.
17. A coloured substrate which is obtainable by a process as defined in any one of claims 3 to 6.
18. An item of packaging, a lens or a security tag which incorporates a coloured substrate as defined in claim 17.
19. A compound of formula (I) : wherein each of A and B, which are the same or different, is an aryl or heteroaryl ring; n is an integer of 1 to 3; y is 0 or an integer of 1 to 5; z is 0 or an integer of 1 to 5; provided that z and y are not both 0;
V is - alk, -O-alk-, -alk-O- or-O-alk-O-, wherein alk is C1-C1O alkylene;
W is a functional group of one of the following formulae (a) to (c):
(a) X
Il
C
— NH^ SCH2
(b) Il
C
- CH2' NNH —
(C) X
— NH' NNH — wherein X is O, S or NH2 +; and
R is selected from H, C1-C6 alkyl which is unsubstituted or substituted, aryl which is unsubstituted or substituted, and heteroaryl which is unsubstituted or substituted; provided that the compound is not
4,4'-bis(iV-acetyl-2-aminoethyl)diphenyldiazomethane.
wherein y is an integer of 1 to 5 and each of V, W and R is as defined in claim 19.
21. A compound according to claim 19 or 20 wherein y is 1.
22. A compound according to claim 19 which is of the following formula (Ib):
wherein each of V, W and R is as defined in claim 19.
23. A process for producing a compound of formula (VII) or (VIII) :
wherein A is an aryl or heteroaryl ring;
B is an aryl or heteroaryl ring; p is 1, 2, 3, 4 or 5; r is 1, 2, 3, 4 or 5;
R1 is selected from hydrogen, aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C1-C10 alkoxy which is substituted or unsubstituted, C1-C1O alkylamino which is substituted or unsubstituted, di(C1-C1o)alkylamino which is substituted or unsubstituted, C1-C1O alkylthio which is substituted or unsubstituted, and C1- C10 alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R2), O or S wherein R2 is H or C1-C6 alkyl;
Q2, which may be the same as or different from Q, is -N(Z1)(Z2); and
Z1 and Z2 are independently selected from aryl which is unsubstituted or substituted, heteroaryl which is unsubstituted or substituted, C1-CiO alkoxy which is substituted or unsubstituted, C1-C10 alkylamino which is substituted or unsubstituted, di(C1-C1o)alkylamino which is substituted or unsubstituted, CpCio alkylthio which is substituted or unsubstituted, and C1-C10 alkyl which is unsubstituted or substituted and which is optionally interrupted by N(R ), O or S wherein R is H or Ci-C6 alkyl; the process comprising treating a compound of formula (V) or (VI) with hydrazine in the presence of heat and a solvent:
wherein A, B, Q, Q2, p, r and R1 are as defined above.
24. A process according to claim 23, which process further comprises oxidising a compound of formula (VII) or (VIII) to produce a carbene precursor compound of formula (III) or (IV) respectively:
wherein A, B, Q, Q2, p, r and R1 are as defined in claim 23.
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JP2007550850A JP5068177B2 (en) | 2005-01-17 | 2006-01-17 | Surface functionalization using aryl carbene reactive intermediates |
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WO2008023170A1 (en) * | 2006-08-23 | 2008-02-28 | Isis Innovation Limited | Tailored control of surface properties by chemical modification |
EP2174582A1 (en) | 2008-10-09 | 2010-04-14 | Invendo Medical Gmbh | Medical, elastic polymer hose and method for production of same |
EP2734574A4 (en) * | 2011-07-18 | 2015-11-04 | Celgard Llc | Surface modified polymeric materials, modified functionalized polymers, functional polymers, and methods |
US9193678B2 (en) | 2009-03-02 | 2015-11-24 | Oxford Advanced Surfaces Ltd | Chemical agents capable of forming covalent 3-D networks |
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GB2497791B (en) | 2011-12-21 | 2021-01-20 | Schlumberger Holdings | Derivatization of carbon |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR1500512A (en) * | 1965-10-23 | 1967-11-03 | Archer Daniels Midland Co | Process for the modification by a carbene of surfaces into an organic substance |
WO2000026180A1 (en) * | 1998-11-03 | 2000-05-11 | Isis Innovation Limited | Surface functionalisation |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CH636093A5 (en) | 1978-01-28 | 1983-05-13 | Ciba Geigy Ag | MICROBICIDAL AGENTS. |
ZA941727B (en) * | 1993-03-12 | 1994-10-26 | Univ Auburn | Polymeric cyclic N-halamine biocidal compounds. |
US5811460A (en) | 1994-01-24 | 1998-09-22 | The Regents Of The University Of California | Water soluble fullerenes with antiviral activity |
AUPM666694A0 (en) * | 1994-07-06 | 1994-07-28 | Unisearch Limited | Natural antifouling compositions |
GB9609919D0 (en) * | 1996-05-11 | 1996-07-17 | Zeneca Ltd | Chemical compounds |
AUPO772097A0 (en) | 1997-07-04 | 1997-07-31 | Aquaculture Crc Limited | Antifouling polymers |
-
2005
- 2005-01-17 GB GBGB0500895.8A patent/GB0500895D0/en not_active Ceased
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2006
- 2006-01-17 ES ES06700684T patent/ES2372699T3/en active Active
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR1500512A (en) * | 1965-10-23 | 1967-11-03 | Archer Daniels Midland Co | Process for the modification by a carbene of surfaces into an organic substance |
WO2000026180A1 (en) * | 1998-11-03 | 2000-05-11 | Isis Innovation Limited | Surface functionalisation |
US6699527B1 (en) * | 1998-11-03 | 2004-03-02 | Isis Innovation Limited | Process for surface functionalization of polymeric substrates using diaryl carbenes as reactive intermediates |
US20040127691A1 (en) * | 1998-11-03 | 2004-07-01 | Moloney Mark Gerard | Process for surface functionalsation of polymeric substrates using diaryl carbenes as reactive intermediates |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008023170A1 (en) * | 2006-08-23 | 2008-02-28 | Isis Innovation Limited | Tailored control of surface properties by chemical modification |
JP2010501536A (en) * | 2006-08-23 | 2010-01-21 | アイシス・イノベーション・リミテッド | Adjustment control of surface characteristics by chemical modification |
CN101558106B (en) * | 2006-08-23 | 2012-10-17 | 埃西斯创新有限公司 | Tailored control of surface properties by chemical modification |
US8530212B2 (en) | 2006-08-23 | 2013-09-10 | Isis Innovation Limited | Carbene precursor compound for producing an adhesive surface on a substrate |
KR101479122B1 (en) * | 2006-08-23 | 2015-01-07 | 아이시스 이노베이션 리미티드 | Tailored control of surface properties by chemical modification |
EP2174582A1 (en) | 2008-10-09 | 2010-04-14 | Invendo Medical Gmbh | Medical, elastic polymer hose and method for production of same |
EP2174579A1 (en) | 2008-10-09 | 2010-04-14 | Invendo Medical Gmbh | Medical, elastic polymer hose and method for production of same |
DE102008042718A1 (en) | 2008-10-09 | 2010-04-15 | Invendo Medical Gmbh | Medical engineering, elastic polymer tube and method for its production |
US9193678B2 (en) | 2009-03-02 | 2015-11-24 | Oxford Advanced Surfaces Ltd | Chemical agents capable of forming covalent 3-D networks |
EP2734574A4 (en) * | 2011-07-18 | 2015-11-04 | Celgard Llc | Surface modified polymeric materials, modified functionalized polymers, functional polymers, and methods |
US10069126B2 (en) | 2011-07-18 | 2018-09-04 | Celgard, Llc | Surface modified polymeric materials, modified functionalized polymers, functional polymers, and methods |
US10665839B2 (en) | 2011-07-18 | 2020-05-26 | Celgard, Llc | Surface modified polymeric materials, modified functionalized polymers, functional polymers, and methods |
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EP1846484A2 (en) | 2007-10-24 |
PL1846484T3 (en) | 2012-02-29 |
DK1846484T3 (en) | 2011-10-24 |
US7939581B2 (en) | 2011-05-10 |
ATE523550T1 (en) | 2011-09-15 |
US20080146731A1 (en) | 2008-06-19 |
JP5068177B2 (en) | 2012-11-07 |
GB0500895D0 (en) | 2005-02-23 |
WO2006075183A3 (en) | 2006-09-14 |
EP1846484B1 (en) | 2011-09-07 |
JP2008526942A (en) | 2008-07-24 |
ES2372699T3 (en) | 2012-01-25 |
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