WO2006000869A2

WO2006000869A2 - System for marking a biological object and for taking a sample of the biological object

Info

Publication number: WO2006000869A2
Application number: PCT/IB2005/001698
Authority: WO
Inventors: Roy Caisley
Original assignee: Nehls, Reinhard
Priority date: 2004-06-29
Filing date: 2005-06-17
Publication date: 2006-01-05
Also published as: NL1027712C1; CA2572184A1; EP1781086A2; US20080044313A1; EP1781086B1; WO2006000869A3; DE602005008257D1; EA009803B1; EA200700157A1; ATE400999T1

Abstract

A system for marking a biological object and taking a sample of the biological object, which object is in particular an animal. The system comprises a first tag part (1), a second tag part (2) and a pin (3), having a first end and a second end, which pin is connected to the first tag part by its first end and which pin is provided with a head (4) at its second end. The second tag part is provided with a receiving opening (5) for the head of the pin, such that the pin can be pressed through a part of the biological object, in particular through the ear of an animal, and subsequently through the receiving opening of the second tag part, as a result of which the first and second tag parts are attached to one another and to the biological object via the pin. The system furthermore comprises a sample container with an opening for introducing a sample of biological material of the object into the sample system for marking a biological object and taking a sample of the biological object, which object is in particular an animal. The system comprises a first tag part, a second tag part pin, having a first end and a second end, which pin is connected to the first tag part by its first end and which pin is provided with a head at its second end. The second tag part is provided with a receiving opening for the head of the pin, such that the pin ca be pressed through a part of the biological object, in particular through the ear of an animal, and subsequently through the receiving opening of the second tag part, as a result of which the first and second tag parts are attached to one another and to the biological object via the pin. The system furthermore comprises a sample container with an opening for introducing a sample of biological material of the object into the sample container, which sample container has a body with a peripheral wall and an opening at an axial end and a bottom at the opposite axial end. The sample container (10) is attached to the second tag part via a releasable connection, in such a manner that the opening of the sample container and the receiving opening of the second tag part lie in line. The system furthermore comprises a stopper (20) for sealing the opening after the sample has been introduced into the sample container, which stopper is on the head of the pin. While the pin is being pushed through the biological object, a sample of the biological object is taken and introduced into the sample container, following which the opening of the sample container is sealed by the stopper. The stopper is at least partially transparent, such that the introduction of a sample into the sample container can be checked visually.

Description

SYSTEM FOR MARKING A BIOLOGICAL OBJECT AND FOR TAKING A SAMPLE OF THE BIOLOGICAL OBJECT.

The invention relates to marking of a biological object and taking a sample of the biological object. In particular, the invention relates to marking and sampling of animals, especially for reliable identification of each individual animal. In the past few years, the desire and need to reliably identify individual animals has been increasing further. Particularly if the animals' meat is intended for human consumption or if the animals are going to be part of the human consumption chain in some other way, there is a great need for reliable identification. One example is the BSE problem, where the accurate determination of the origin and the life history of the animals is of great importance. However, the reliable identification of animals also plays a part if the animal involved is not to be consumed, but is a pet, (race) horse, or the like. However, the invention also relates to the marking and sampling of other biological objects, such as plants. The invention relates to a system which not only provides for an ear tag to be attached to the animal, but also more or less simultaneously taking a sample of biological material from the animal. The sample of biological material is then intended in particular for the identification of the animal based on the "genetic fingerprint" which can be determined using the sample taken. Of course, the sample can also be used for other purposes. Such systems are known, inter alia, from WO 02/39810 and US 6,509,187. With known systems of this type, there is a risk that no sample material will end up in the sample container when the tag is being attached. As it is conceivable that such sample containers containing samples will not be analyzed immediately, but will be stored for a prolonged period of time, and if appropriate only "problematic cases" are analyzed, there is a risk that the fact that no sample has been taken will only be discovered at a late stage. This could, for example, be not until a long time after the animal in question has been slaughtered, which means that no new sample can be taken either. In order to solve this problem, WO 02/078431 proposes enabling a "visual inspection" of the contents of the sample container if the latter is closed off by the stopper. With this known system, the stopper has a receiving cavity surrounded by a cutting edge on its front side. The sample container is designed to be at least partially transparent in order to carry out the visual inspection, preferably near the top of the stopper in the closed state of the sample container. In particular, it is proposed to construct the sample container from transparent plastic and to partially coat the latter with a layer of paint, with a readable identification code being applied on the painted part. It is mentioned that this code would be difficult to read if there were no paint coat. This known system has turned out to be unsatisfactory in practice. In particular, it has emerged that it is possible with the known sample container, that visually the impression is given that a usable sample has been taken, whereas this is found not to be the case during subsequent analysis. Furthermore, the known system is not attractive in terms of production engineering, in particular regarding the application of the paint coat. It is an object of the present invention to provide an improved system in order to solve one or more of the abovementioned drawbacks. The invention provides a system according to the preamble of claim 1, which is characterized in that the stopper is at least partially transparent, such that the introduction of a sample into the sample container can be checked visually. This measure makes it possible to check the contents of the sample container via the stopper. This is advantageous as the sample is just on the top of the stopper, optionally in a receiving cavity provided for this purpose in the top of the stopper. The check can therefore be carried out from an optimum direction. Preferably, only the stopper is designed to be transparent and the body of the sample container is completely non-transparent and preferably produced by injection-moulding from a non-transparent plastic material. This is particularly advantageous if a readable identification is applied to the exterior of the sample container. The invention also relates to a stopper with a transparent part for sealing a sample container of a system of this type. Such stoppers are sometimes supplied premounted on the pin, but likewise as a separate component which is placed on the pin shortly before the tag is attached. Further advantageous embodiments are described in the subclaims and in the following description with reference to the drawing, in which: Fig. 1 shows, in combination with the sample container and the stopper, a first ear tag part provided with an integral pin and a second ear tag part to which the sample container is releasably attached; Fig. 2 shows a sample container and the associated stopper of the system according to the invention; Fig. 3 shows the release of the closed sample container from the ear tag attached to the ear (not shown) ; Fig. 4 shows the sealed sample container in section; Fig. 5 shows an alternative sample container provided with a stopper having a lens, which is surrounded by a break-away edge; and Fig. 6 shows another alternative stopper with sample container. Below, an exemplary embodiment of the system for marking a biological object, in particular for ear-marking an animal, and for taking a sample of the biological object according to the invention will be described with reference to Figures 1-4. In this context, it is pointed out that those skilled in the art can take possible embodiments and information regarding the method of operation from WO 02/39810, the contents of which are deemed to be incorporated in this application by way of reference. The system illustrated comprises a first tag part 1, a second tag part 2 and a pin 3. The pin 3 has a first end and a second end, the pin 3 being connected to the first tag part 1 by its first end. The pin is provided with a head 4 at its second end. The second tag part 2 is provided with a receiving opening 5 for the head 4 of the pin 3, such that the pin 3 can be pressed through a part of the biological object, in particular through the ear of an animal, and subsequently through the receiving opening 5 of the second tag part 2, as a result of which the first and second tag parts 1, 2 are attached to one another and to the biological object via the pin 3. The system furthermore comprises a sample container 10 with an opening 11 (see Fig. 2) for introducing a sample of biological material of the object into the sample container 10, which sample container 10 is attached to the second tag part 2 (see Fig. 1) via a releasable connection, in such a manner that the opening 11 of the sample container 10 and the receiving opening 5 of the second tag part 2 lie in line. In the embodiment shown, a flange edge 12 of the sample container 10 is releasably held in a raised annular wall 7 of the second tag part 2. The system furthermore comprises a stopper 20 for sealing the opening 11 after the sample has been introduced into the sample container 10. While the pin 3 is being pushed through the object, the stopper 20 is on the head 4 of the pin 3, in such a manner that a sample of the biological object is taken while the pin is being pushed through the biological object and said sample is introduced into the sample container 10, following which the opening 11 of the sample container 10 is directly sealed by the stopper 20. At the same time, the stopper 20 may detach from the head 4 of the pin 3. In the embodiment shown, the stopper 20 has a protruding annular wall 21 at its front, which delimits a cavity 22 which is open at the front and in which sample material 30 will land (see Fig. 4) . In the embodiment illustrated in the drawing, the sample container 10 comprises a body, for example produced by injection- moulding a suitable plastic, with a peripheral wall 14 and the opening 11 at one axial end and an integrally formed bottom 15 at the opposite axial end. The body of the sample container 10 is non-transparent, for example made from POM or polyamide. The stopper 20 is transparent in order that the introduction of a sample 30 into the sample container 10 can be checked visually. The stopper is, for example, made from PC (polycarbonate) , PS (polystyrene) , PMMA. In one embodiment which is not shown and is known per se, the opening 11 of the sample container 10 is initially sealed by a releasable seal, for example by a pierceable diaphragm, for example a plastic sheet. Such a releasable seal could be provided on the sample container, but possibly also on the second ear tag part. A releasable seal of this type may serve to initially protect the sample container from the environment and thereby prevent contamination. Another reason may be that one or more substances are accommodated in the sample container, for example a substance which is intended for processing the sample further and/or for preserving the sample. Such a substance, for example a preservative, could also be provided in the stopper. Below, a sample container with an alternative stopper will be explained with reference to Figure 5. In this example, the stopper 40 shown is largely or, if desired, completely made of a transparent plastic material. The stopper 40 has a stop edge 41, with which the stopper 40 bears against the body of the sample container 45 in the filled and closed state of the sample container 45. In this example, the interaction between the stop edge 41 and the sample container 45 prevents the stopper 40 from being pushed in any further. Furthermore, the stopper 40 has a transparent section 42 which is designed as a lens, in this example a lens 42 which magnifies when looking from the outside in. The lens 42 is in this case also a bottom of a space 46 which is surrounded by the cutting ring 44 on the head of the stopper 40. The lens 42 affords a better view of the contents of the sample container than if that section 42 were a transparent part of substantially uniform thickness. It should be noted that the cutting ring 44 may, if desired, be made of a suitable metal, which improves the cutting action. Using the lens 42, it is easy to check, for example using the naked eye, whether any ear material has landed in the sample container 45, immediately after the ear tag is fitted. After all, if this were not the case and checking would not be easy, it would only be discovered at a late stage that no sample had been taken. This is particularly problematic if an animal has already been slaughtered, as then no new samples can be taken. It can furthermore be seen from Fig. 5 that a break-away edge 43 extends around the lens 42, as a result of which the lens 42 in this case also forms a part of the stopper 40 which can be broken away. When a considerable pressure force is exerted on the lens 42, for example using a suitable tool, the lens 42 breaks away from the rest of the stopper 40. This makes it possible to push the lens 42 inwards when the sample is being analyzed and thus to gain access to the collected sample. The non-transparent body of the sample container 10, 45 may be provided with a readable identification on its exterior, for example a 2D bar code. The stopper could be made from two elements, so that it has a body with an element made of non-transparent material and an element made of transparent material. In a variant (not shown) , the bottom 15 of the sample container 10 is transparent and the peripheral wall is non-transparent, so that it is possible to check whether a sample is present by means of a beam through the opposite bottom 15 and stopper 20. This could for example be advantageous, if an automatic inspection of the sample container is carried out, during which a suitable light beam is passed through the bottom and the stopper. Also, said transparent bottom 15 could be designed to be a lens. Figure 6 shows a variant of the stopper 40, in which the stopper 40 has a substantially tubular part 40a, in this example having a metal cutting ring 44 at the front end, and a transparent part 40b in the bore of the part 40a, which transparent part 40b is fixed therein. In this example, a chamber 47 is present in the stopper 40, in this case an annular chamber 47 bounded by the parts 40a and 40b, in which a preserving substance or other substance (s) is (are) contained which aids in preserving and/or further processing the sample to be taken. This chamber 47 is in communication with the cavity 46 for the sample to be taken. It will be clear that the presence of a substance of this type in the chamber 47 has no adverse effects with respect to checking the sample. The annular chamber 47, containing the transparent part of the stopper which offers a view of the cavity 46, can also be implemented with other embodiments of the stopper.

Claims

C L A I M S

1. System for marking a biological object and taking a sample of the biological object, which object is in particular an animal, comprising: - a first tag part (1) , - a second tag part (2) - a pin (3) , having a first end and a second end, which pin is connected to the first tag part by its first end and which pin is provided with a head (4) at its second end, - in which the second tag part is provided with a receiving opening (5) for the head (4) of the pin, such that the pin can be pressed through a part of the biological object, in particular through the ear of an animal, and subsequently through the receiving opening of the second tag part (2) , as a result of which the first and second tag parts are attached to one another and to the biological object via the pin, which system furthermore comprises: a sample container (10) with an opening (11) for introducing a sample of biological material of the object into the sample container, which sample container (10) is attached to the second tag part (2) via a releasable connection, in such a manner that the opening (11) of the sample container and the receiving opening (5) of the second tag part lie in line, a stopper (20; 40) for sealing the opening (11) after the sample has been introduced into the sample container (10;45), which stopper (20; 40) is on the head (4) of the pin (3) when the tag parts are being fitted to the biological object, in such a manner that a sample of the biological object is taken while the pin is being pushed through the biological object and said sample is introduced into the sample container, following which the opening (11) of the sample container is sealed by the stopper (20) , in which it is possible to check visually whether a sample has been introduced into the sample container after the sample container has been closed, characterized in that the stopper (20; 40) is at least partially transparent, such that the introduction of a sample into the sample container (10; 45) can be checked visually via the transparent stopper.

2. System according to claim 1, in which the body of the sample container is non-transparent and the stopper (20; 40) is at least partially transparent.

3. System according to claim 2, in which the sample container (10;45) has a body with a peripheral wall (14) and the opening (11) for the stopper at one axial end and a bottom (15) at the opposite axial end, in which the body is non-transparent and the stopper (20;40) is at least partially transparent.

4. System according to claim 1, in which the sample container has a body with a peripheral wall and the opening for the stopper at one axial end and a bottom at the opposite axial end, in which the peripheral wall is non-transparent and the bottom of the sample container is transparent.

5. System according to one or more of the preceding claims, in which the stopper has a body with an element made of a non- transparent material and an element made of transparent material.

6. System according to one or more of the preceding claims, in which a transparent part of the stopper (40) is designed as a lens (42), preferably a magnifying lens (42) .

7. System according to one or more of the preceding claims, in which a transparent part of the stopper (40) has a break-away part (42) which is bounded by a break-away edge (43), which breaks away from the rest of the stopper when a considerable pressure force is exerted on that break-away part, for example using a suitable tool.

8. System according to one or more of the preceding claims, in which the stopper has a cavity (14; 46) surrounded by a cutting ring (22;44) at its front end, for example a metal cutting ring (44), for receiving the sample (30) .

9. System according to claims 6 and 8, in which the lens (42) forms the bottom of the cavity (46) for receiving the sample.

10. System according to claim 8 or 9, in which the cutting ring (44) is made of metal.

11. Stopper for a system according to one or more of the preceding claims, which stopper is at least partially transparent.

12. Method for marking a biological object and taking a sample of the biological object, in which use is made of a system according to one or more of the preceding claims, and in which the introduction of the sample into the sample container is checked via the transparent stopper.

13. Method for manipulating, for example removing and/or analyzing, a sample accommodated in the sample container of the system according to claim 7, in which a considerable pressure is exerted on the break-away part using a suitable tool, so that this part breaks away.