WO2003097861A1 - Method and device for detecting toxic material in water using microbial fuel cell - Google Patents
Method and device for detecting toxic material in water using microbial fuel cell Download PDFInfo
- Publication number
- WO2003097861A1 WO2003097861A1 PCT/KR2003/000854 KR0300854W WO03097861A1 WO 2003097861 A1 WO2003097861 A1 WO 2003097861A1 KR 0300854 W KR0300854 W KR 0300854W WO 03097861 A1 WO03097861 A1 WO 03097861A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- fuel cell
- water
- sample
- toxic materials
- microbial fuel
- Prior art date
Links
- 239000000463 material Substances 0.000 title claims abstract description 49
- 231100000331 toxic Toxicity 0.000 title claims abstract description 44
- 230000002588 toxic effect Effects 0.000 title claims abstract description 44
- 239000000446 fuel Substances 0.000 title claims abstract description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 230000000813 microbial effect Effects 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 15
- 244000005700 microbiome Species 0.000 claims abstract description 8
- 231100000419 toxicity Toxicity 0.000 claims description 12
- 230000001988 toxicity Effects 0.000 claims description 12
- 238000012216 screening Methods 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 238000012423 maintenance Methods 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 230000005611 electricity Effects 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 241000251468 Actinopterygii Species 0.000 description 11
- 238000001514 detection method Methods 0.000 description 9
- 241000238571 Cladocera Species 0.000 description 5
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 239000012086 standard solution Substances 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000594011 Leuciscus leuciscus Species 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000011368 organic material Substances 0.000 description 2
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012806 monitoring device Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
Definitions
- This invention relates to the detecting method of the toxic materials by a biological means and the device thereof. More specifically, this invention relates to an automatic detecting method of the toxic materials using microbial fuel cell and an automatic alarming device thereof.
- Representative conventional biological detecting devices for toxic materials in water include monitoring methods by using fish, water flea and fluorescent microorganism.
- the device using fish for water quality monitoring takes advantage of the character of the fish to swim against the flow of water, namely it uses the countercurrent phenomenon for detection. After an anti-escape net is installed and when toxic materials are introduced through the inlet, the fish is effected and its swimming activity slows down. The effected fish is pushed back due to the current, yet the fish, by instinct, will move tail fin vigorously in order to move forward again, the tail fin touching the sensor during the process. This action is transformed into electric signals and recorded. The value of this electric signals are detected by the water quality monitoring device and is used to give alarm or in controlling the flow rate of water by the connected controller.
- the fish used for the purpose is usually a golden dace belonging to the dace genera in the carp family.
- the shortfalls of using the fish to detect the toxic material is that the object of detecting the toxicity is so large that it requires 8 hours to determine the toxicity when 8ppm phenol is introduced to water.
- the method of using fish for biological toxicity alarm system has low sensibility, and the detection time and the error range are wide. The selection and the growing condition of fish reduces reproducibility and uniformity of the alarming system.
- the device using water flea for the toxic material senses the activity of the water fleas by infrared sensor.
- the test chamber and the various tubes for the input/output of water are either washed or exchanged. Care and efforts are needed in growing the fleas, because the growing water should be prepared and exchanged for 2-3 times a week, and progeny and parent fleas should be carefully separated.
- the fleas are reared in a special growing chamber where its space is disinfected and any equipments interfering with the growth are eliminated. Fresh air must be supplied to the growing chamber.
- the device for automatic detection of the toxicity in water using the fixed fluorescent microorganism, determines the fluorescence against the toxicity. It needs various light detecting equipments, making it costly and requiring personnel for their maintenance and an expert for them as well.
- the present invention is proposed based on the fact that the problems for such conventional automatic detection devices for water toxicity arise eventually from the sensor parts. Disclosure of the Invention
- the objective of the present invention is to solve the technical problems in the conventional automatic determination devices for detecting toxicity in water and to provide fast and correct determination method of toxicity with low cost and easy maintenance.
- the objective of the present invention is accomplished by the method for detecting the toxic materials in a sample using electrochemically active microorganism.
- the objective is achieved by the method for detecting the toxic materials in water, characterized in that it comprises the steps of: determining the electrochemical signals generated from the microbial fuel cell; introducing a sample into the above-mentioned microbial fuel cell; and determining the degree of changes in the electrochemical signals generated from the above-mentioned microbial fuel cell.
- the method for detecting toxic materials in water can comprise further a step of screening out the suspension and unwanted materials in the sample before introducing the sample to the above microbial fuel cell.
- the device for detecting the above-mentioned toxic materials comprises a pump taking a sample; a pretreatment tank treating the sample; a microbial fuel cell sensing the changes in the current by the introduced toxic materials; and a PC and a controlling part which control the signal values and automatically determine the toxicity.
- Figure 1 is a schematic illustration of the automatic determination device for toxic materials comprising a pump(l) taking a sample; a pretreatment tank(2) treating the sample; a microbial fuel cell(6) sensing the changes in the current occurred by the introduced toxic materials; and a PC and a controlling part(ll) which control the value of the signals and automatically determine the toxicity.
- the device comprises a solenoid valve(5) which makes changes of flow of the sample when the toxic materials are introduced and sample-gathering vessel(4) which gathers and stores the sample at a point when the signal is recognized.
- the following illustration is the working mechanism of the device with the abovementioned constitution for determining toxic material by the use of the microbial fuel cell.
- the sample enters into the anode part after passing through the first and the second pretreatment tanks(2,3).
- the anode part is composed of a carbon felt and platinum(Pt) wire, while its inside part is filled usually with a microorganism catalyst which generates electrochemical energy using organic raw material.
- the cathode part is filled with ordinary water. Namely, the sample containing organic materials enters into the anode and the water-saturated with air enters the cathode. Now the organic materials are decomposed by microorganism at the anode part of the fuel cell and a current is generated.
- the current moves along the Pt wire and is measured by the voltmeter.
- the electric current does not show any range of changes, however, once the toxic material enters into the anode part, metabolism of the electrochemically active microorganism is slowed down, making the abrupt drop in the voltage.
- Such sharp drop of the current is processed by the PC and the controlling part, making the alarm activated in the audio/video display.
- Figure 1 is a schematic diagram of the device for detecting the toxic materials.
- Figure 2 is the graph showing the result of Embodiment 1.
- Figure 3 is the graph showing the result of Embodiment 2.
- Figure 4 is the graph showing the result of Embodiment 4.
- Figure 5 is the graph showing the result of Embodiment 5.
- Second pretreatment tank for sample (3) Second pretreatment tank for sample (4) Sample-gathering vessel (5) Solenoid valve (6) Microbial fuel cell (7) Sample outlet (8) Tap water chamber
- Active sludge was introduced into the anode part so that the electrochemically active bacteria in the sludge are attached to the electrode and densely cultured.
- water saturated with air was incorporated, keeping a certain potential difference so as to make an efficient biological electrochemical reaction occur in the microbial fuel cell.
- glucose and glutamic acid(CODcr 200ppm which means the chemical oxygen demand due to the potassium dichromate) as fuel and the generated current was measured by the volt meter(2000 multimeter, Keithley Instrument.
- the same fuel cell and the fuel as in the Embodiment 1 was used.
- the generated amount of current was measured by the volt meter(2000 multimeter, Keithley Instrument. Inc, USA) at 60 seconds interval, while adding to the used fuel cell, mercury(Hg) standard solution successively in the concentration of O.Olppm, 0.02ppm, 0.03ppm, 0.04ppm and 0.05ppm.
- the result showed as in Embodiment 1 that the current generated in a constant rate showed a sharp drop at 0.03ppm of Hg. (Fig. 3)
- Embodiment 3 the same fuel cell and the fuel as in the Embodiment 1 was used. After feeding the fuel, the generated amount of current was measured by the volt meter(2000 multimeter, Keithley Instrument. Inc, USA) at 60 seconds interval, while adding to the used fuel cell, lead(Pb) standard solution successively in the concentration of O.Olppm, 0.02ppm, 0.03ppm, 0.04ppm and 0.05ppm. The result showed as in Embodiment 1 that the current generated in a constant rate showed a sharp drop at 0.04ppm ofPb. (Fig. 4)
- the same fuel cell and the fuel as in the Embodiment 1 was used.
- the generated amount of current was measured by the volt meter(2000 multimeter, Keithley Instrument. Inc, USA) at 60 seconds interval, while adding to the used fuel cell, phenol standard solution successively in the concentration of O.Olppm, 0.02ppm, 0.03ppm, 0.04ppm and 0.05ppm.
- the result showed as in Embodiment 1 that the current generated in a constant rate showed a sharp drop at 0.03ppm of phenol. (Fig. 5)
- the toxic materials when the toxic materials are incorporated into the sample to be tested, there is an abrupt drop of the generated electricity by the electrochemically active bacteria in the microbial fuel cell, maximizing the sensitivity in detecting the toxic materials.
- Use of the microbial fuel cell minimizes the cost and the personnel in managing and maintaining the sensor part as well as remarkably enhancing the reproducibility and the degree of accuracy in detection of the toxic materials compared with the conventional alarming device.
- the present invention minimizes the damage by detecting the inflow of the toxic material in the early stage.
- Development of such excellent devices of detecting toxic materials contributes effectively to the national economy in that the related devices can be exported, replacing importation, once they are locally produced.
- the detecting device of the toxic materials using microbial fuel cell controls the degree of biological toxicity of the waste and sewage water and is used to detect rapidly the pollution of the intake water source of the drinking water.
- the device When the device is installed in the protected region of the water source, it can effectively prevent in advance the illegal disposal of the polluted materials by the industries and industrial complex facilities.
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/509,718 US20050164331A1 (en) | 2002-04-27 | 2003-04-26 | Method and device for detecting toxic material in water using microbial fuel cell |
DE60334291T DE60334291D1 (en) | 2002-04-27 | 2003-04-26 | METHOD AND DEVICE FOR DETECTING TOXIC MATERIAL IN WATER USING A MICROBIAL FUEL CELL |
EP03721117A EP1497451B1 (en) | 2002-04-27 | 2003-04-26 | Method and device for detecting toxic material in water using microbial fuel cell |
AU2003224472A AU2003224472A1 (en) | 2002-04-27 | 2003-04-26 | Method and device for detecting toxic material in water using microbial fuel cell |
AT03721117T ATE482284T1 (en) | 2002-04-27 | 2003-04-26 | METHOD AND DEVICE FOR DETECTING TOXIC MATERIAL IN WATER USING A MICROBIAL FUEL CELL |
JP2004506516A JP4469271B2 (en) | 2002-04-27 | 2003-04-26 | Method and system for confirming presence of toxic substance in sample using electrochemically active microorganism |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2002-0023232 | 2002-04-27 | ||
KR10-2002-0023232A KR100483580B1 (en) | 2002-04-27 | 2002-04-27 | Method for sensing toxic material in water using microorganism cell |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2003097861A1 true WO2003097861A1 (en) | 2003-11-27 |
Family
ID=29546278
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2003/000854 WO2003097861A1 (en) | 2002-04-27 | 2003-04-26 | Method and device for detecting toxic material in water using microbial fuel cell |
Country Status (9)
Country | Link |
---|---|
US (1) | US20050164331A1 (en) |
EP (1) | EP1497451B1 (en) |
JP (1) | JP4469271B2 (en) |
KR (1) | KR100483580B1 (en) |
CN (1) | CN1300330C (en) |
AT (1) | ATE482284T1 (en) |
AU (1) | AU2003224472A1 (en) |
DE (1) | DE60334291D1 (en) |
WO (1) | WO2003097861A1 (en) |
Cited By (7)
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---|---|---|---|---|
NL1033432C2 (en) * | 2007-02-20 | 2008-08-21 | Stichting Wetsus Ct Of Excelle | Method for determining the toxicity of a sample stream and apparatus therefor. |
FR2931845A1 (en) * | 2008-05-27 | 2009-12-04 | Centre Nat Rech Scient | PRODUCTION OF A BIOFILM ON AN ELECTRODE FOR BIOPILE, ELECTRODE AND BIOPILE OBTAINED. |
WO2010010313A2 (en) * | 2008-07-25 | 2010-01-28 | Nanotec Solution | Single-use biomass sensor device, method for producing same, and single-use bioreactor with said sensor built therein |
US20150053577A1 (en) * | 2013-08-25 | 2015-02-26 | Alan Joseph Bauer | Devices and methods for identifying a biological or chemical residue in an aqueous sample |
EP2510345A4 (en) * | 2009-12-08 | 2015-07-08 | Cambrian Innovation Inc | Microbially-based sensors for environmental monitoring |
US10833340B2 (en) | 2013-11-01 | 2020-11-10 | Lockheed Martin Energy, Llc | Apparatus and method for determining state of charge in a redox flow battery via limiting currents |
US11150213B2 (en) | 2011-06-14 | 2021-10-19 | Cambrian Innovation Inc. | Biological oxygen demand sensors |
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CN101882350B (en) * | 2010-05-24 | 2012-05-16 | 清华大学 | Water pollution organism early warning system and method based on principle of microbial fuel cells |
US10851003B2 (en) * | 2010-07-21 | 2020-12-01 | Matthew Silver | Denitrification and pH control using bio-electrochemical systems |
US10099950B2 (en) | 2010-07-21 | 2018-10-16 | Cambrian Innovation Llc | Bio-electrochemical system for treating wastewater |
WO2012054629A2 (en) | 2010-10-19 | 2012-04-26 | Cambrian Innovation | Bio-electrochemical systems |
CN102636545B (en) * | 2012-03-16 | 2013-11-06 | 北京航空航天大学 | Early warning device for biomass with comprehensive toxicity in water |
CN102778485A (en) * | 2012-07-12 | 2012-11-14 | 北京航空航天大学 | Bioelectrochemical device for quickly detecting food comprehensive toxcity |
CN103336045B (en) * | 2013-06-08 | 2015-06-10 | 内蒙古科技大学 | Device for on-line detection and automatic alarm of toxic substances and detection method of toxic substances |
CN103399051A (en) * | 2013-07-31 | 2013-11-20 | 华南理工大学 | Water body toxicity detecting method and portable device based on microbial electrochemical signals |
US20150335184A1 (en) * | 2014-05-26 | 2015-11-26 | Suhasini Balachandran | Smart Container |
CN106290527A (en) * | 2015-05-21 | 2017-01-04 | 北京化工大学 | A kind of water quality toxicity monitoring method based on anaerobic pretreatment Yu air cathode MFC |
CN105537261B (en) * | 2015-12-22 | 2018-06-12 | 中国石油大学(华东) | A kind of restorative procedure of electric power-fuel cell coupling device and contaminated soil |
CN109642886B (en) * | 2016-06-29 | 2022-05-03 | 新加坡国立大学 | Toxicant monitoring system |
CN109115858A (en) * | 2018-09-27 | 2019-01-01 | 北京雪迪龙科技股份有限公司 | A kind of device and method of quick detection soil comprehensive toxicity |
CN111007132A (en) * | 2018-10-05 | 2020-04-14 | 科奥比株式会社 | Integrated system for simultaneously measuring concentration and toxicity of organic matters in water by using electrochemical active bacteria through sensor signal mode analysis method |
CN113109532B (en) * | 2021-04-14 | 2022-11-25 | 齐鲁工业大学 | Water quality monitoring device based on microbial fuel cell |
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2002
- 2002-04-27 KR KR10-2002-0023232A patent/KR100483580B1/en active IP Right Grant
-
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- 2003-04-26 AU AU2003224472A patent/AU2003224472A1/en not_active Abandoned
- 2003-04-26 AT AT03721117T patent/ATE482284T1/en not_active IP Right Cessation
- 2003-04-26 CN CNB038077698A patent/CN1300330C/en not_active Expired - Lifetime
- 2003-04-26 WO PCT/KR2003/000854 patent/WO2003097861A1/en active Application Filing
- 2003-04-26 JP JP2004506516A patent/JP4469271B2/en not_active Expired - Fee Related
- 2003-04-26 US US10/509,718 patent/US20050164331A1/en not_active Abandoned
- 2003-04-26 EP EP03721117A patent/EP1497451B1/en not_active Expired - Lifetime
- 2003-04-26 DE DE60334291T patent/DE60334291D1/en not_active Expired - Lifetime
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Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008103028A1 (en) * | 2007-02-20 | 2008-08-28 | Stichting Wetsus Centre Of Excellence For Sustainable Water Technology | Method of determining the toxicity of a sample flow, and apparatus therefor |
NL1033432C2 (en) * | 2007-02-20 | 2008-08-21 | Stichting Wetsus Ct Of Excelle | Method for determining the toxicity of a sample stream and apparatus therefor. |
WO2009153499A3 (en) * | 2008-05-27 | 2010-04-08 | Centre National De La Recherche Scientifique (C.N.R.S.) | Production of a biofilm on an electrode for a biocell, electrode and biocell obtained |
FR2931845A1 (en) * | 2008-05-27 | 2009-12-04 | Centre Nat Rech Scient | PRODUCTION OF A BIOFILM ON AN ELECTRODE FOR BIOPILE, ELECTRODE AND BIOPILE OBTAINED. |
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Also Published As
Publication number | Publication date |
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JP2005521431A (en) | 2005-07-21 |
AU2003224472A1 (en) | 2003-12-02 |
CN1646696A (en) | 2005-07-27 |
EP1497451A1 (en) | 2005-01-19 |
JP4469271B2 (en) | 2010-05-26 |
CN1300330C (en) | 2007-02-14 |
EP1497451B1 (en) | 2010-09-22 |
US20050164331A1 (en) | 2005-07-28 |
KR20030084486A (en) | 2003-11-01 |
DE60334291D1 (en) | 2010-11-04 |
EP1497451A4 (en) | 2006-07-05 |
KR100483580B1 (en) | 2005-04-18 |
ATE482284T1 (en) | 2010-10-15 |
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