WO2002101036A1 - Methode de formation d'un complexe stable d'un transcrit d'adn codant un peptide arbitraire a l'aide d'un produit de traduction, construction d'acide nucleique utilisee dans cette methode, complexe obtenu par cette methode et criblage de proteine fonctionnelle et marn ou adn codant la proteine a l'aide de cette methode - Google Patents

Methode de formation d'un complexe stable d'un transcrit d'adn codant un peptide arbitraire a l'aide d'un produit de traduction, construction d'acide nucleique utilisee dans cette methode, complexe obtenu par cette methode et criblage de proteine fonctionnelle et marn ou adn codant la proteine a l'aide de cette methode Download PDF

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Publication number
WO2002101036A1
WO2002101036A1 PCT/JP2002/004582 JP0204582W WO02101036A1 WO 2002101036 A1 WO2002101036 A1 WO 2002101036A1 JP 0204582 W JP0204582 W JP 0204582W WO 02101036 A1 WO02101036 A1 WO 02101036A1
Authority
WO
WIPO (PCT)
Prior art keywords
dna encoding
protein
mrna
screening
nucleic acid
Prior art date
Application number
PCT/JP2002/004582
Other languages
English (en)
French (fr)
Inventor
Kazunari Taira
Satoshi Fujita
Shinya Yoshizaki
Masaki Warashina
Original Assignee
Kazunari Taira
Satoshi Fujita
Shinya Yoshizaki
Masaki Warashina
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kazunari Taira, Satoshi Fujita, Shinya Yoshizaki, Masaki Warashina filed Critical Kazunari Taira
Priority to US10/477,373 priority Critical patent/US20050191626A1/en
Priority to EP02724764A priority patent/EP1394250A1/en
Priority to JP2003503786A priority patent/JP4106441B2/ja
Publication of WO2002101036A1 publication Critical patent/WO2002101036A1/ja

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
PCT/JP2002/004582 2001-05-11 2002-05-10 Methode de formation d'un complexe stable d'un transcrit d'adn codant un peptide arbitraire a l'aide d'un produit de traduction, construction d'acide nucleique utilisee dans cette methode, complexe obtenu par cette methode et criblage de proteine fonctionnelle et marn ou adn codant la proteine a l'aide de cette methode WO2002101036A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/477,373 US20050191626A1 (en) 2001-05-11 2002-05-10 Method for forming a stable complex comprising a transcription product and translation product of a dna encoding a desired polypeptide, a nucleic acid construct used for the method, a complex formed by the method, and screening of a functional protein and mrna or dna encoding the protein using the method
EP02724764A EP1394250A1 (en) 2001-05-11 2002-05-10 Method of forming stable complex of transcription product of dna encoding arbitrary peptide with translation product, nucleic acid construct to be used in the method, complex formed by the method ; and screening of functional protein and mrna or dna encoding the protein using the method
JP2003503786A JP4106441B2 (ja) 2001-05-11 2002-05-10 任意のポリペプチドをコードするＤＮＡの転写産物と翻訳産物との安定的な複合体を形成させる方法、該方法に用いる核酸構築物、該方法により形成される複合体、並びに該方法を利用した機能性タンパク質および該タンパク質をコードするｍＲＮＡまたはＤＮＡのスクリーニング

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2001-141594 2001-05-11
JP2001141594 2001-05-11
JP2002062751 2002-03-07
JP2002-62751 2002-03-07

Publications (1)

Publication Number Publication Date
WO2002101036A1 true WO2002101036A1 (fr) 2002-12-19

Family

ID=26614971

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/004582 WO2002101036A1 (fr) 2001-05-11 2002-05-10 Methode de formation d'un complexe stable d'un transcrit d'adn codant un peptide arbitraire a l'aide d'un produit de traduction, construction d'acide nucleique utilisee dans cette methode, complexe obtenu par cette methode et criblage de proteine fonctionnelle et marn ou adn codant la proteine a l'aide de cette methode

Country Status (4)

Country Link
US (1) US20050191626A1 (ja)
EP (1) EP1394250A1 (ja)
JP (1) JP4106441B2 (ja)
WO (1) WO2002101036A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009149549A (ja) * 2007-12-19 2009-07-09 Japan Science & Technology Agency ペプチドアプタマーライブラリーの作製方法および用途
JPWO2013046960A1 (ja) * 2011-09-29 2015-03-26 Ｎｅｃソリューションイノベータ株式会社 ペプチド抗体のスクリーニングに使用する核酸構築物およびそれを用いたスクリーニング方法
JP5804520B2 (ja) * 2010-04-01 2015-11-04 Ｎｅｃソリューションイノベータ株式会社 核酸構築物、それを用いた複合体の製造方法およびスクリーニング方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008041607A1 (fr) 2006-10-02 2008-04-10 Panasonic Electric Works Co., Ltd. Capteur de pression
EP3629022A1 (en) 2008-07-25 2020-04-01 Richard W. Wagner Protein screening methods
WO2017189409A1 (en) * 2016-04-25 2017-11-02 Evorx Technologies, Inc. Beta-catenin barcoded peptides

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0340948B1 (en) * 1988-04-28 1995-06-28 Mycogen Corporation Novel hybrid pesticidal toxins
EP0466222B1 (en) * 1990-06-11 1999-09-08 Dow AgroSciences LLC Ribosome-inactivating proteins, inactive precursor forms thereof, a process for making and a method of using
WO1999057265A1 (en) * 1998-05-04 1999-11-11 Julianna Lisziewicz Chimeric decoy rnas having synergistic anti-hiv activity
WO2001027263A1 (fr) * 1999-10-08 2001-04-19 National Insutitute Of Advanced Industrial Science And Technology Aptamere module et procede de detection d'une proteine cible a l'aide de celui-ci

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0340948B1 (en) * 1988-04-28 1995-06-28 Mycogen Corporation Novel hybrid pesticidal toxins
EP0466222B1 (en) * 1990-06-11 1999-09-08 Dow AgroSciences LLC Ribosome-inactivating proteins, inactive precursor forms thereof, a process for making and a method of using
WO1999057265A1 (en) * 1998-05-04 1999-11-11 Julianna Lisziewicz Chimeric decoy rnas having synergistic anti-hiv activity
WO2001027263A1 (fr) * 1999-10-08 2001-04-19 National Insutitute Of Advanced Industrial Science And Technology Aptamere module et procede de detection d'une proteine cible a l'aide de celui-ci

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JING-MIN ZHOU ET AL.: "A novel strategy by the action of ricin that connects phenotype and genotype without loss of the diversity of libraries", J. AM. CHEM. SOC., vol. 124, no. 4, 2002, pages 538 - 543, XP002956621 *
SCHAFFITZEL CHRISTIANE ET AL.: "Ribosome display: an in vitro method for selection and evolution of antibodies from libraries", J. IMMUNOL. METHODS, vol. 231, 1999, pages 119 - 135, XP004186079 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009149549A (ja) * 2007-12-19 2009-07-09 Japan Science & Technology Agency ペプチドアプタマーライブラリーの作製方法および用途
JP5804520B2 (ja) * 2010-04-01 2015-11-04 Ｎｅｃソリューションイノベータ株式会社 核酸構築物、それを用いた複合体の製造方法およびスクリーニング方法
JPWO2013046960A1 (ja) * 2011-09-29 2015-03-26 Ｎｅｃソリューションイノベータ株式会社 ペプチド抗体のスクリーニングに使用する核酸構築物およびそれを用いたスクリーニング方法

Also Published As

Publication number Publication date
JP4106441B2 (ja) 2008-06-25
JPWO2002101036A1 (ja) 2004-09-24
EP1394250A1 (en) 2004-03-03
US20050191626A1 (en) 2005-09-01

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