WO2002018530A1 - Surfactant system - Google Patents
Surfactant system Download PDFInfo
- Publication number
- WO2002018530A1 WO2002018530A1 PCT/AU2001/001094 AU0101094W WO0218530A1 WO 2002018530 A1 WO2002018530 A1 WO 2002018530A1 AU 0101094 W AU0101094 W AU 0101094W WO 0218530 A1 WO0218530 A1 WO 0218530A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- alkyl
- pyrrolidone
- weight
- composition according
- composition
- Prior art date
Links
- 239000004094 surface-active agent Substances 0.000 title description 14
- -1 alkyl pyrrolidone Chemical compound 0.000 claims abstract description 56
- 239000000203 mixture Substances 0.000 claims abstract description 56
- 102000004190 Enzymes Human genes 0.000 claims abstract description 37
- 108090000790 Enzymes Proteins 0.000 claims abstract description 37
- 229940088598 enzyme Drugs 0.000 claims abstract description 37
- 125000000217 alkyl group Chemical group 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 24
- 238000004140 cleaning Methods 0.000 claims abstract description 23
- 239000005017 polysaccharide Substances 0.000 claims abstract description 20
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 20
- 108091005804 Peptidases Proteins 0.000 claims abstract description 16
- 102000035195 Peptidases Human genes 0.000 claims abstract description 16
- 239000004365 Protease Substances 0.000 claims abstract description 14
- 108010065511 Amylases Proteins 0.000 claims abstract description 5
- 102000013142 Amylases Human genes 0.000 claims abstract description 5
- 239000004367 Lipase Substances 0.000 claims abstract description 5
- 102000004882 Lipase Human genes 0.000 claims abstract description 5
- 108090001060 Lipase Proteins 0.000 claims abstract description 5
- 235000019418 amylase Nutrition 0.000 claims abstract description 5
- 235000019421 lipase Nutrition 0.000 claims abstract description 5
- 230000002708 enhancing effect Effects 0.000 claims abstract description 3
- 102000005575 Cellulases Human genes 0.000 claims abstract 3
- 108010084185 Cellulases Proteins 0.000 claims abstract 3
- 229940025131 amylases Drugs 0.000 claims abstract 3
- 239000012141 concentrate Substances 0.000 claims description 37
- 239000000243 solution Substances 0.000 claims description 20
- NJPQAIBZIHNJDO-UHFFFAOYSA-N 1-dodecylpyrrolidin-2-one Chemical group CCCCCCCCCCCCN1CCCC1=O NJPQAIBZIHNJDO-UHFFFAOYSA-N 0.000 claims description 19
- 238000010790 dilution Methods 0.000 claims description 16
- 239000012895 dilution Substances 0.000 claims description 16
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 9
- 239000002736 nonionic surfactant Substances 0.000 claims description 4
- HNJBEVLQSNELDL-UHFFFAOYSA-N pyrrolidin-2-one Chemical compound O=C1CCCN1 HNJBEVLQSNELDL-UHFFFAOYSA-N 0.000 claims description 2
- 230000000694 effects Effects 0.000 description 23
- 239000000523 sample Substances 0.000 description 23
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 20
- 239000007983 Tris buffer Substances 0.000 description 18
- 239000002689 soil Substances 0.000 description 17
- 238000002360 preparation method Methods 0.000 description 14
- 239000000758 substrate Substances 0.000 description 12
- 239000000654 additive Substances 0.000 description 11
- 235000019419 proteases Nutrition 0.000 description 11
- 238000009472 formulation Methods 0.000 description 10
- 239000011550 stock solution Substances 0.000 description 10
- 230000000996 additive effect Effects 0.000 description 9
- 238000003556 assay Methods 0.000 description 9
- 239000003599 detergent Substances 0.000 description 9
- 235000013336 milk Nutrition 0.000 description 8
- 239000008267 milk Substances 0.000 description 8
- 210000004080 milk Anatomy 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 108010041102 azocasein Proteins 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 239000011521 glass Substances 0.000 description 7
- 239000008233 hard water Substances 0.000 description 6
- 235000013372 meat Nutrition 0.000 description 6
- 108010083608 Durazym Proteins 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 5
- 239000008367 deionised water Substances 0.000 description 5
- 108010020132 microbial serine proteinases Proteins 0.000 description 5
- 102000002322 Egg Proteins Human genes 0.000 description 4
- 108010000912 Egg Proteins Proteins 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 235000013345 egg yolk Nutrition 0.000 description 4
- 210000002969 egg yolk Anatomy 0.000 description 4
- 108010003855 mesentericopeptidase Proteins 0.000 description 4
- 230000002797 proteolythic effect Effects 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 108010010803 Gelatin Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 108010056079 Subtilisins Proteins 0.000 description 3
- 102000005158 Subtilisins Human genes 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 235000015278 beef Nutrition 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000008273 gelatin Substances 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
- 235000019322 gelatine Nutrition 0.000 description 3
- 235000011852 gelatine desserts Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- WPPOGHDFAVQKLN-UHFFFAOYSA-N N-Octyl-2-pyrrolidone Chemical compound CCCCCCCCN1CCCC1=O WPPOGHDFAVQKLN-UHFFFAOYSA-N 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 108090000787 Subtilisin Proteins 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000004035 construction material Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000004851 dishwashing Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 150000004040 pyrrolidinones Chemical class 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005201 scrubbing Methods 0.000 description 1
- 210000002374 sebum Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000012414 sterilization procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 108010075550 termamyl Proteins 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- DCXXMTOCNZCJGO-UHFFFAOYSA-N tristearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC DCXXMTOCNZCJGO-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/32—Organic compounds containing nitrogen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/662—Carbohydrates or derivatives
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/26—Organic compounds containing nitrogen
- C11D3/28—Heterocyclic compounds containing nitrogen in the ring
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
Definitions
- the invention relates to a surfactant system for use in aqueous compositions and more particularly to a surfactant system for use in, or addition to, aqueous enzyme based cleaning preparations.
- the invention has particular advantage when employed in enzyme based cleaning preparations intended for use for cleaning medical instruments.
- compositions for cleaning medical instruments are intended broadly to include surgical instruments such as scalpels, biopsy instruments, clamps, and the like; endoscopes, colonoscopes, laparoscopes and other paraphernalia used for surgical investigation or intervention; and other instruments used in the practice of medicine, surgery, and dentistry which require cleaning, disinfection or sterilisation.
- the term is also intended to include instruments having similar cleaning requirements such as those used in hairdressing, cosmetic treatments, tattooing, body piercing etc as well as other applications where removal of human/animal sebum and/or body secretion is required.
- Many medical instruments are made from or incorporate a variety of construction materials and cleaning compositions must be such as not to attack any such materials.
- Preparations for use in cleaning medical instruments consist generally of a concentrate comprising one or more surfactants in combination with one or more proteolytic enzymes.
- the concentrate is diluted to a working strength (normally a hundredfold dilution) prior to use.
- a working strength normally a hundredfold dilution
- One such formulation has been described by NONO ⁇ ORDISK (" ⁇ ovo-formulation").
- Three preparations used internationally for this purpose are EPIZYME ® a product of 3M, MEDIZYME ® (a product of Whiteley Industries Pty Ltd., Sydney Australia) and E ⁇ DOZYME ® (a product of Ruholf Corporation, ⁇ Y, USA). While such products are generally effective and widely used there remains a need for products which are more efficient, and especially for preparations which are capable of achieving a predetermined level of effectiveness within a shorter time than those currently available.
- the concentrate must be sufficiently stable in transit and storage prior to use to satisfy the needs of commerce.
- the choice of surfactants which in practice can be formulated in concentrated aqueous solutions is limited. Either the surfactants limit the storage stability of the concentrate, resulting in problems of foaming on dilution, provide insufficient reduction in surface tension, destabilize other components of the system such as enzymes, or suffer from other disadvantages.
- formulators utilize surfactants chosen from a small group of conventional and well known nonionic surfactants which have been found to be satisfactory for such uses, but there remains an unsatisfied demand for formulations of improved efficiency.
- the invention provides an aqueous composition comprising in combination an alkyl pyrrolidone and an alkyl polysaccharide.
- alkyl pyrrolidone is a C8 - C18 linear alkyl pyrrolidone.
- N-DP N- dodecyl pyrrolidone
- the alkyl polysaccharide is an alkyl polyglucoside (“APG").
- the invention provides a method of enhancing the efficacy of an enzyme containing composition for use in cleaning medical instruments comprising the step of including an alkyl pyrrolidone and an alkyl polysaccharide.
- the composition is a concentrate which contains surfactant according to the first aspect as part of its formulation, but the surfactant system can be added subsequently either to the concentrate or the working solution.
- the invention provides a method of cleaning a medical instrument including the step of treating the instrument with a composition including at least one enzyme, an alkyl pyrrolidone and an alkyl polysaccharide.
- Alkylpyrrolidones are known to reduce surface tension progressively with increasing concentration in water achieving a minimum surface tension (maximum effectiveness) at a concentration of about 0.1% in the case of dodecyl pyrrolidone and about 0.002% in the case of octyl pyrrolidone, which concentrations correspond to the maximum solubility of the respective pyrrolidones in water. This has in the past inhibited their use in concentrates, since the maximum concentration in an aqueous system is inevitably less than fully effective as a surfactant when diluted to any substantial degree.
- n-dodecyl pyrrolidone n- DP
- alkyl polyglucoside MEDIZYME ®
- ENDOZYME ® alkyl polyglucoside
- EPIZYME ® n-dodecyl pyrrolidone
- NONO formulation a product described by ⁇ ONO INDUSTRIES in Novo Nordisk Publications Application Sheet B-613, "Application of Enzymes in Detergents for Endoscope Cleaning" was also prepared and tested.
- n-DP n-dodecyl pyrrolidone
- APG alkyl polyglucoside
- the NOVO Formulation (Concentrate) is as follows:
- Non-ionic surfactant (Teric BL9) 12
- the Epizyme composition (concentrate) is as follows:
- the protease may be subtilisin, the amylase Alpha-amylase, the cellulase may be endo-l,4-beta-glucanase and the lipase may be Triacylglycerol.
- the cleaning composition concentrates were diluted at a rate of 1 part of concentrate in 100 parts of standard hard water (0.304 g calcium chloride and 0.065g of magnesium chloride per 1L of distilled water. Therapeutic Goods Order 54 & Guidelines, 1996, p. 17).
- the efficacy of each product was then tested against standard soil preparations on glass slides, (1) neat and (2) with the addition of an additive according to the invention.
- the efficiency of the preparation was measured at various temperatures. The results are shown in Table 1 in comparison with results of compositions including the additive according to the invention.
- N-DP N-dodecyl pyrrolidone (Surfadone LP 300 from International Speciality
- APG Alkyl polyglucoside (ALKADET 15 from Huntsman Corporation Australia)
- Dilution rate 1 part of concentrate in 100 parts of standard hard water
- the addition of the additive reduces the time required for the composition to achieve a given score and does so at each temperature.
- the additive is especially effective at lower temperatures.
- the ratio of N-DP to APG can be varied. It is preferred that the ratio be between 1 part by weight of alkyl pyrrolidone to 3 parts by weight of alkyl polyglucoside and 3 parts by weight of alkyl pyrrolidone to 1 part by weight of alkyl polyglucoside, more preferably between 3 parts by weight of alkyl pyrrolidone to 1 parts by weight of alkyl polyglucoside and 1 part by weight of alkyl pyrrolidone to 1 part by weight of alkyl polyglucoside.
- the concentration of N-DP and APG is each preferably less than 7.5% w/w of the concentrate ( ie less than about .0075%in the concentrate at dilution of lpart concentrate: 100 parts water) and more preferably is less than 3% w/w.
- concentrations employed will depend on the surface tension properties required, the hydrophilicity of the alkyl polyglucosides and the degree of hydrotroping provided by other excipients in the formulation to the alkyl polyglucoside, as well as other factors which may occur with respect to individual formulations.
- Table 2 shows the effect of the additive in accelerated aging tests. The results demonstrate that the additive slightly improves storage stability, or, at least, does not adversely affect it.
- Table 3 shows the effects of varying the concentration and ratio of the alkylpyrrolidone and alkylpolyglucoside. It can be seen that the composition is efficacious in reducing contact times for cleaning over a wide range of alkylpyrroline to alkylpolyglucoside ratios.
- Soil carrier glass microscope slides
- SURF n-dodecyl pyrrolidone (Surfadone LP 300 from International Speciality Products),
- APG Alkyl polyglucoside (ALKADET 15 from Huntsman Corporation Australia)
- Soil carrier glass microscope slides
- SURF n-dodecyl pyrrolidone (Surfadone LP 300 from International Speciality Products),
- APG Alkyl polyglucoside (ALKADET 15 from Huntsman Corporation Australia) Addition rate: 0.2 w/w% SURF and 7.5% APG to concentrate Dilution rate: 1 part of concentrate in 100 parts of standard hard water Results.
- Soil carrier glass microscope slides
- SURF n-dodecyl pyrrolidone (Surfadone LP 300 from International Speciality Products),
- APG Alkyl polyglucoside (ALKADET 15 from Huntsman Corporation Australia) Addition rate: 1% w/w% SURF and 0.1% APG to concentrate Dilution rate: 1 part of concentrate in 100 parts of standard hard water Results.
- Soil carrier glass microscope slides
- SURF n-dodecyl pyrrolidone (Surfadone LP 300 from International Speciality Products),
- APG Alkyl polyglucoside (ALKADET 15 from Huntsman Corporation Australia) Addition rate: 7.5% w/w% SURF and 0.1% APG to concentrate Dilution rate: 1 part of concentrate in 100 parts of standard hard water Assessed using B 863B test method by ⁇ ONO NORDISK (attached) TABLE 4
- the microwave was pre-heated by placing 6 water-filled beakers in a circle on the glass turn-table and leaving it on "high" (900 W) for 10 minutes. Then, the beakers with water were replaced by beakers with 10 ml of milk. The milk was heated for 10 minutes at 450 W, then allowed to cool, forming a skin, and placed into an oven for 2 hours at 80°C.
- Mince meat -20 grams of beef mince
- the mince was mixed with the egg yolk and water with the use of a hand blender, lg of homogenized mixture was spooned onto 3x3 cm ceramic tiles, onto the smooth side. Then the tiles were left in an oven for 10 minutes.
- the beef gelatin and water were blended together and heated until the mixture obtained a homogenous consistency. Cholesterol, hog mucin and egg yolk were blended in after gelatin was cooled to 30C. 0.1 g of soil was deposited onto surfaces of the microscope slides. The smears were made of a uniform thickness for each set of slides; the thickness was regulated by a border of adhesive tape along each border of the smearing instrument In order to simulate the conditions of an endoscope cleaning, the slides were left to dry in open air at 20°C for 20 minutes.
- the polysaccharide appears to stabilize the pyrrolidone, the combination being more soluble in the concentrate and resulting in substantially improved lowering of surface tension at the working dilution.
- test results utilize n-dodecyl pyrrolidone
- other alkyl pyrrolidones could be substituted therefore, and especially C8 to C18 alkyl pyrrolidones.
- the amount to be added can be determined by simple trial but in general increasing the concentration significantly above the levels herein employed tends to give a decreasing advantage.
- other alkyl polysaccharides may be substituted for APG.
- other sugar units and derivatives of sugar units may be substituted for glucose units.
- n-alkyl pyrrolidone with APG is highly preferred, beneficial effects are obtainable with at least some soils by adding it in isolation.
- the optimum ratio of n-DP to APG can be determined by simple trial for a given concentrate.
- the combination of the invention provides an improved surfactant system which is expected to have application in many end-uses.
- the surfactant system of the invention may be simply added to commercially available enzyme cleaning products either to the concentrate or, at an appropriate dilution to the working solution prior to use.
- the additives are incorporated into a concentrate at the time of manufacture of the concentrate which is then merely diluted prior to use.
- Additives according to the invention may be added to dishwashing, hand washing and other surface cleaning compositions.
- Embodiments of the invention may be altered and may have other chemicals added or substituted therefore to an extent which will be apparent to those skilled in the art from the teaching hereof without departing from the inventive concept herein disclosed.
- FIG. 1 A diagrammatic representation of this method is shown in figure 1.
- protease is allowed to hydrolyse azocasein for 30 minutes at 40°C. Undigested protein is precipitated with trichloroacetic acid and the quantity of digested product is determined by spectrophotometry.
- Activity is determined relative to a standard of known activity and the result given in the same units as used for the standard.
- This concentrate is to be used at 10 ml/100 ml volume for all final dilutions with 0.2 M Tris buffer.
- the pH of this solution be checked and, if needed, adjusted to 8.5 • 0.1 units. If it is desired to know the exact activity levels of proteinase in a detergent, the detergent bases should be included in the standard solutions unless it is already known that the particular formulation being used has no effect on the protease activity. The concentration of the base detergent should be equal to that used in the sample solution of the detergent containing protease.
- Activity/gram (activity/tube) • ( " dilution factor gram of sample
- Anson Unit the amount of enzyme* which digests haemoglobin A an initial rate such that there is liberated per minute an amount of TCA-soluble product which gives the same colour with phenol reagent as one milliequivalent of tyrosine. * Under reaction conditions given in the NNAS method AF4/5-GB: Modified Anson— Haemoglobin Method for the Determination of Proteolytic Activity.
- a standard curve is prepared using an enzyme whose activity in Anson Units is known.
- Buffer 0.2 M Tris, pH 8.5
- Standard curve Enzyme standard stock solution Dissolve accurately- weighed Alcalase standard in 100 ml 0.2 M Tris buffer (pH 8.5) to yield 2.6 • 10 "3 AU/ml
- KNPU Kilo Novo Protease Unit
- 1 Novo Protease Unit the amount of enzyme* which hydrolyzes casein at such a rate that the initial rate of formation of peptides/minute corresponds to 1 micromole of glycine/minute
- Buffer 0.2 M Tris, pH 8.5
- Enzyme standard stock solution Dissolve accurately-weighed Savinase or Esperase standard in 100 mil 0.2 M Tris buffer (pH 8.5) to yield 1»10 "2 KNPU/ml.
- E Esperase
- S Savinase APPENDIX III
- DPU Durazym Protease Unit
- Sample preparation Dissolve an accurately- weighed amount of sample in an accurately-known volume of 0.2 M Tris buffer so that the solution contains between ⁇ lO "4 -- 18»10 "4 DPU/ml and has apH of 8.5. N.B. For each unknown, prepare a sample blank and one or more duplicates (as necessary).
- Enzyme standard stock solution Dissolve accurately-weighed Durazym standard in 100ml 0.2 M Tris buffer (pH 8.5) to yield 1.10 "2 DPU/ml
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Abstract
Description
Claims
Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2421035A CA2421035C (en) | 2000-09-01 | 2001-08-30 | A surfactant system comprising alkyl pyrrolidone and alkyl polysaccharide |
EP01964733A EP1313832B1 (en) | 2000-09-01 | 2001-08-30 | Surfactant system |
NZ524912A NZ524912A (en) | 2000-09-01 | 2001-08-30 | Use of an alkyl pyrrolidone and an alkyl polysaccharide combination to enhance the efficacy of an enzyme |
BRPI0113783A BRPI0113783B8 (en) | 2000-09-01 | 2001-08-30 | aqueous composition |
DK01964733T DK1313832T3 (en) | 2000-09-01 | 2001-08-30 | surfactant |
JP2002524033A JP4896355B2 (en) | 2000-09-01 | 2001-08-30 | Surfactant system |
DE60123812T DE60123812T2 (en) | 2000-09-01 | 2001-08-30 | surfactant |
KR1020037003182A KR100853257B1 (en) | 2000-09-01 | 2001-08-30 | Surfactant system |
AU8557901A AU8557901A (en) | 2000-09-01 | 2001-08-30 | Surfactant system |
US10/377,294 US6939836B2 (en) | 2000-09-01 | 2003-02-27 | Surfactant system |
HK03107871A HK1055604A1 (en) | 2000-09-01 | 2003-10-31 | Surfactant system |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AUPQ9844 | 2000-09-01 | ||
AUPQ9844A AUPQ984400A0 (en) | 2000-09-01 | 2000-09-01 | Surfactant sytem |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/377,294 Continuation US6939836B2 (en) | 2000-09-01 | 2003-02-27 | Surfactant system |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002018530A1 true WO2002018530A1 (en) | 2002-03-07 |
WO2002018530A9 WO2002018530A9 (en) | 2002-09-06 |
Family
ID=3823898
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/AU2001/001094 WO2002018530A1 (en) | 2000-09-01 | 2001-08-30 | Surfactant system |
Country Status (19)
Country | Link |
---|---|
US (1) | US6939836B2 (en) |
EP (1) | EP1313832B1 (en) |
JP (2) | JP4896355B2 (en) |
KR (2) | KR100853257B1 (en) |
CN (1) | CN1247754C (en) |
AR (1) | AR030593A1 (en) |
AT (1) | ATE342328T1 (en) |
AU (2) | AUPQ984400A0 (en) |
BR (1) | BRPI0113783B8 (en) |
CA (1) | CA2421035C (en) |
DE (1) | DE60123812T2 (en) |
DK (1) | DK1313832T3 (en) |
ES (1) | ES2275718T3 (en) |
HK (1) | HK1055604A1 (en) |
MY (1) | MY140244A (en) |
NZ (1) | NZ524912A (en) |
PT (1) | PT1313832E (en) |
TW (1) | TWI289602B (en) |
WO (1) | WO2002018530A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004094080A1 (en) * | 2003-04-22 | 2004-11-04 | Novapharm Research (Australia) Pty Ltd | Endoscope cleaning pad |
US6939836B2 (en) * | 2000-09-01 | 2005-09-06 | Novapharm Research (Australia) Pty Ltd | Surfactant system |
EP2032680A1 (en) * | 2006-06-01 | 2009-03-11 | 3M Innovative Properties Company | Cleaning composition |
AU2004232373B2 (en) * | 2003-04-22 | 2010-07-29 | Novapharm Research (Australia) Pty Ltd | Endoscope cleaning pad |
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JP4633482B2 (en) * | 2005-01-17 | 2011-02-16 | 花王株式会社 | Medical disinfectant cleaning composition |
US7491362B1 (en) * | 2008-01-28 | 2009-02-17 | Ecolab Inc. | Multiple enzyme cleaner for surgical instruments and endoscopes |
US8846595B2 (en) * | 2008-07-14 | 2014-09-30 | 3M Innovative Properties Company | Method of making a cleaning solution from hydrogel cleaning concentrate and packaged cleaning concentrate |
DE102014003484A1 (en) | 2014-03-14 | 2015-09-17 | Bode Chemie Gmbh | Cleaner for inanimate surfaces with special effectiveness against mucus, secretions, blood and biofilms |
CN106456813B (en) * | 2014-04-15 | 2020-06-30 | 勃林格殷格翰国际公司 | Method, apparatus and system for continuous inactivation of viruses during manufacture of biological products |
KR20160067714A (en) | 2014-12-04 | 2016-06-14 | 주식회사 엘지화학 | Copolycarbonate and article containing the same |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5558806A (en) * | 1992-07-23 | 1996-09-24 | Osi Specialties, Inc. | Surfactant blend of a polyalkleneoxide polysiloxane and an organic compound having a short chain hydrophobic moiety |
US5846926A (en) * | 1997-06-09 | 1998-12-08 | Rhodia Inc. | Nonionic gemini surfactants with three hydrophilic heads and two lipophilic tails |
US5863886A (en) * | 1997-09-03 | 1999-01-26 | Rhodia Inc. | Nonionic gemini surfactants having multiple hydrophobic and hydrophilic sugar groups |
US5908619A (en) * | 1997-01-09 | 1999-06-01 | Minnesota Mining And Manufacturing Company | Hydroalcoholic compositions thickened using surfactant/polymer complexes |
US5922663A (en) * | 1996-10-04 | 1999-07-13 | Rhodia Inc. | Enhancement of soil release with gemini surfactants |
US6040288A (en) * | 1997-02-21 | 2000-03-21 | Rhodia Inc. | Fabric color protection compositions and methods |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5093031A (en) * | 1986-06-27 | 1992-03-03 | Isp Investments Inc. | Surface active lactams |
DE3816734A1 (en) * | 1988-05-17 | 1989-11-30 | Henkel Kgaa | METHOD FOR CLEANING AND DISINFECTING HEAT AND CORROSION-SENSITIVE MEDICAL DEVICES, ESPECIALLY ENDOSCOPES, AND MEANS FOR IMPLEMENTING THE METHOD |
US5468423A (en) * | 1992-02-07 | 1995-11-21 | The Clorox Company | Reduced residue hard surface cleaner |
JPH0899842A (en) * | 1994-10-03 | 1996-04-16 | Nippon Fine Chem Co Ltd | Foam-like acidic hair-dyeing agent |
JPH10330792A (en) * | 1997-05-28 | 1998-12-15 | T Paul Kk | Bactericidal detergent composition and its use |
EP1076570A1 (en) * | 1998-05-01 | 2001-02-21 | The Procter & Gamble Company | Method for sanitizing medical equipment using microwaves |
US6159916A (en) * | 1998-06-12 | 2000-12-12 | The Clorox Company | Shower rinsing composition |
JP2000087093A (en) * | 1998-09-09 | 2000-03-28 | Shiseido Co Ltd | Detergent composition |
EP1122302A1 (en) * | 2000-01-31 | 2001-08-08 | Henkel Kommanditgesellschaft auf Aktien | Treatment of soiled textiles |
AUPQ984400A0 (en) * | 2000-09-01 | 2000-09-28 | Novapharm Research (Australia) Pty Ltd | Surfactant sytem |
-
2000
- 2000-09-01 AU AUPQ9844A patent/AUPQ984400A0/en not_active Abandoned
-
2001
- 2001-08-30 BR BRPI0113783A patent/BRPI0113783B8/en not_active IP Right Cessation
- 2001-08-30 KR KR1020037003182A patent/KR100853257B1/en active IP Right Grant
- 2001-08-30 NZ NZ524912A patent/NZ524912A/en not_active IP Right Cessation
- 2001-08-30 DK DK01964733T patent/DK1313832T3/en active
- 2001-08-30 WO PCT/AU2001/001094 patent/WO2002018530A1/en active IP Right Grant
- 2001-08-30 KR KR1020087010323A patent/KR100894491B1/en active IP Right Grant
- 2001-08-30 DE DE60123812T patent/DE60123812T2/en not_active Revoked
- 2001-08-30 ES ES01964733T patent/ES2275718T3/en not_active Expired - Lifetime
- 2001-08-30 AT AT01964733T patent/ATE342328T1/en not_active IP Right Cessation
- 2001-08-30 EP EP01964733A patent/EP1313832B1/en not_active Revoked
- 2001-08-30 PT PT01964733T patent/PT1313832E/en unknown
- 2001-08-30 JP JP2002524033A patent/JP4896355B2/en not_active Expired - Lifetime
- 2001-08-30 CA CA2421035A patent/CA2421035C/en not_active Expired - Lifetime
- 2001-08-30 CN CNB018160506A patent/CN1247754C/en not_active Expired - Lifetime
- 2001-08-30 MY MYPI20014109A patent/MY140244A/en unknown
- 2001-08-31 TW TW090121660A patent/TWI289602B/en not_active IP Right Cessation
- 2001-08-31 AR ARP010104180A patent/AR030593A1/en active IP Right Grant
-
2003
- 2003-02-27 US US10/377,294 patent/US6939836B2/en not_active Expired - Lifetime
- 2003-10-31 HK HK03107871A patent/HK1055604A1/en not_active IP Right Cessation
-
2005
- 2005-06-15 AU AU2005100493A patent/AU2005100493A4/en not_active Expired
-
2011
- 2011-11-02 JP JP2011241263A patent/JP2012072409A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5558806A (en) * | 1992-07-23 | 1996-09-24 | Osi Specialties, Inc. | Surfactant blend of a polyalkleneoxide polysiloxane and an organic compound having a short chain hydrophobic moiety |
US5922663A (en) * | 1996-10-04 | 1999-07-13 | Rhodia Inc. | Enhancement of soil release with gemini surfactants |
US5908619A (en) * | 1997-01-09 | 1999-06-01 | Minnesota Mining And Manufacturing Company | Hydroalcoholic compositions thickened using surfactant/polymer complexes |
US6040288A (en) * | 1997-02-21 | 2000-03-21 | Rhodia Inc. | Fabric color protection compositions and methods |
US5846926A (en) * | 1997-06-09 | 1998-12-08 | Rhodia Inc. | Nonionic gemini surfactants with three hydrophilic heads and two lipophilic tails |
US5863886A (en) * | 1997-09-03 | 1999-01-26 | Rhodia Inc. | Nonionic gemini surfactants having multiple hydrophobic and hydrophilic sugar groups |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6939836B2 (en) * | 2000-09-01 | 2005-09-06 | Novapharm Research (Australia) Pty Ltd | Surfactant system |
WO2004094080A1 (en) * | 2003-04-22 | 2004-11-04 | Novapharm Research (Australia) Pty Ltd | Endoscope cleaning pad |
JP2006524066A (en) * | 2003-04-22 | 2006-10-26 | ノバファーム リサーチ(オーストラリア)プロプライエタリー リミテッド | Endoscope cleaning pad |
AU2004232373B2 (en) * | 2003-04-22 | 2010-07-29 | Novapharm Research (Australia) Pty Ltd | Endoscope cleaning pad |
JP2012096039A (en) * | 2003-04-22 | 2012-05-24 | Novapharm Research (Australia) Pty Ltd | Endoscope cleaning pad |
US8196248B2 (en) | 2003-04-22 | 2012-06-12 | Novapharm Research (Australia) Pty Ltd | Endoscope cleaning pad |
EP2032680A1 (en) * | 2006-06-01 | 2009-03-11 | 3M Innovative Properties Company | Cleaning composition |
EP2032680A4 (en) * | 2006-06-01 | 2010-01-20 | 3M Innovative Properties Co | Cleaning composition |
Also Published As
Publication number | Publication date |
---|---|
ES2275718T3 (en) | 2007-06-16 |
EP1313832A4 (en) | 2004-08-25 |
CA2421035A1 (en) | 2002-03-07 |
HK1055604A1 (en) | 2004-01-16 |
AR030593A1 (en) | 2003-08-27 |
DE60123812T2 (en) | 2007-08-02 |
AUPQ984400A0 (en) | 2000-09-28 |
JP4896355B2 (en) | 2012-03-14 |
TWI289602B (en) | 2007-11-11 |
KR100894491B1 (en) | 2009-04-22 |
EP1313832A1 (en) | 2003-05-28 |
CA2421035C (en) | 2010-09-28 |
US6939836B2 (en) | 2005-09-06 |
CN1462307A (en) | 2003-12-17 |
EP1313832B1 (en) | 2006-10-11 |
JP2012072409A (en) | 2012-04-12 |
BR0113783B1 (en) | 2013-11-12 |
AU2005100493A4 (en) | 2005-07-28 |
MY140244A (en) | 2009-12-31 |
CN1247754C (en) | 2006-03-29 |
BRPI0113783B8 (en) | 2021-07-27 |
WO2002018530A9 (en) | 2002-09-06 |
PT1313832E (en) | 2007-01-31 |
KR100853257B1 (en) | 2008-08-20 |
KR20030031176A (en) | 2003-04-18 |
DE60123812D1 (en) | 2006-11-23 |
DK1313832T3 (en) | 2007-02-19 |
US20030220222A1 (en) | 2003-11-27 |
KR20080042943A (en) | 2008-05-15 |
JP2004506812A (en) | 2004-03-04 |
ATE342328T1 (en) | 2006-11-15 |
NZ524912A (en) | 2004-09-24 |
BR0113783A (en) | 2005-02-01 |
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