WO2002004943A3 - Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery - Google Patents

Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery Download PDF

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Publication number
WO2002004943A3
WO2002004943A3 PCT/US2001/021484 US0121484W WO0204943A3 WO 2002004943 A3 WO2002004943 A3 WO 2002004943A3 US 0121484 W US0121484 W US 0121484W WO 0204943 A3 WO0204943 A3 WO 0204943A3
Authority
WO
WIPO (PCT)
Prior art keywords
compounds
apparatuses
electrophysiology
present
high throughput
Prior art date
Application number
PCT/US2001/021484
Other languages
French (fr)
Other versions
WO2002004943A2 (en
Inventor
Charles D Weaver
Alastair Binnie
Original Assignee
Bristol Myers Squibb Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Priority to IL15354501A priority Critical patent/IL153545A0/en
Priority to CA002413663A priority patent/CA2413663A1/en
Priority to JP2002509762A priority patent/JP2004514417A/en
Priority to EP01950953A priority patent/EP1311848A2/en
Priority to AU2001271898A priority patent/AU2001271898B2/en
Priority to MXPA03000160A priority patent/MXPA03000160A/en
Priority to AU7189801A priority patent/AU7189801A/en
Publication of WO2002004943A2 publication Critical patent/WO2002004943A2/en
Publication of WO2002004943A3 publication Critical patent/WO2002004943A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides apparatuses for measuring cell membrane resistance, conductance, potential, and capacitance, which can be used to determine cellular electrical activity. More specifically, the present invention provides automated apparatuses that are designed for high-throughput analysis of various compounds, ligands, and cell processes that modulate cellular electrical properties (e.g., cell membrane conditions). Such automated apparatuses can be used for the precise determination of ion channel activity, and for the rapid identification of compounds, ligands, or processes that alter this activity. The present invention also provides methods for measuring cellular electrical properties utilizing the apparatuses provided herein.
PCT/US2001/021484 2000-07-07 2001-07-06 Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery WO2002004943A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
IL15354501A IL153545A0 (en) 2000-07-07 2001-07-06 Apparatus for measuring cellular electrical activity
CA002413663A CA2413663A1 (en) 2000-07-07 2001-07-06 Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery
JP2002509762A JP2004514417A (en) 2000-07-07 2001-07-06 Electrophysiological configurations suitable for high-throughput screening of drug discovery compounds
EP01950953A EP1311848A2 (en) 2000-07-07 2001-07-06 Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery
AU2001271898A AU2001271898B2 (en) 2000-07-07 2001-07-06 Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery
MXPA03000160A MXPA03000160A (en) 2000-07-07 2001-07-06 Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery.
AU7189801A AU7189801A (en) 2000-07-07 2001-07-06 Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21690300P 2000-07-07 2000-07-07
US60/216,903 2000-07-07

Publications (2)

Publication Number Publication Date
WO2002004943A2 WO2002004943A2 (en) 2002-01-17
WO2002004943A3 true WO2002004943A3 (en) 2003-03-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/021484 WO2002004943A2 (en) 2000-07-07 2001-07-06 Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery

Country Status (8)

Country Link
US (1) US20020053915A1 (en)
EP (1) EP1311848A2 (en)
JP (1) JP2004514417A (en)
AU (2) AU7189801A (en)
CA (1) CA2413663A1 (en)
IL (1) IL153545A0 (en)
MX (1) MXPA03000160A (en)
WO (1) WO2002004943A2 (en)

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US9146221B2 (en) 2001-03-24 2015-09-29 Aviva Biosciences Corporation High-density ion transport measurement biochip devices and methods
US9399787B2 (en) 2002-12-20 2016-07-26 Acea Biosciences, Inc. Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays

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US20050196746A1 (en) * 2001-03-24 2005-09-08 Jia Xu High-density ion transport measurement biochip devices and methods
US20050058990A1 (en) * 2001-03-24 2005-03-17 Antonio Guia Biochip devices for ion transport measurement, methods of manufacture, and methods of use
US20050009004A1 (en) * 2002-05-04 2005-01-13 Jia Xu Apparatus including ion transport detecting structures and methods of use
US20040146849A1 (en) * 2002-01-24 2004-07-29 Mingxian Huang Biochips including ion transport detecting structures and methods of use
EP1372828A4 (en) * 2001-03-24 2008-10-29 Aviva Biosciences Corp Biochips including ion transport detecting structures and methods of use
DE10202887B4 (en) * 2002-01-25 2004-05-06 Advalytix Ag Cell analysis method
GB0203053D0 (en) * 2002-02-08 2002-03-27 Ayanda Biosystems Sarl Bio-sensors
GB0207845D0 (en) * 2002-04-04 2002-05-15 Lgc Ltd Apparatus, methods and means for biochemical analysis
EP1501924A4 (en) 2002-05-04 2006-05-24 Aviva Biosciences Corp Apparatus including ion transport detecting structures and methods of use
ES2208082B1 (en) * 2002-05-29 2005-09-16 Jose Luis Bardasano Rubio DEVICE FOR MEASURING THE POTENTIAL OF TRANSMEMBRANE ACTION IN CELLULAR PREPARATIONS UNDER THE ACTION OF ELECTROMAGNETIC FIELDS OF VARIABLE FREQUENCY AND INTENSITY.
EP1511843B1 (en) * 2002-06-07 2006-03-29 Sophion Bioscience A/S Screening methods
US7470533B2 (en) 2002-12-20 2008-12-30 Acea Biosciences Impedance based devices and methods for use in assays
WO2004010103A2 (en) 2002-07-20 2004-01-29 Acea Biosciences, Inc. Impedance based devices and methods for use in assays
US7732127B2 (en) 2002-12-20 2010-06-08 Acea Biosciences, Inc. Dynamic monitoring of cell adhesion and spreading using the RT-CES system
US7468255B2 (en) 2002-12-20 2008-12-23 Acea Biosciences Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology
US8206903B2 (en) 2002-12-20 2012-06-26 Acea Biosciences Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
US8263375B2 (en) 2002-12-20 2012-09-11 Acea Biosciences Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
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EP1801586B1 (en) * 2002-08-21 2010-10-13 Cellectricon Ab System for obtaining and maintaining high-resistance seals in patch clamp recordings
US8232074B2 (en) 2002-10-16 2012-07-31 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
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US11346797B2 (en) 2002-12-20 2022-05-31 Agilent Technologies, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
US10215748B2 (en) 2002-12-20 2019-02-26 Acea Biosciences, Inc. Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US10551371B2 (en) 2003-11-10 2020-02-04 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function
US10539523B2 (en) 2002-12-20 2020-01-21 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties
GB0307352D0 (en) * 2003-03-29 2003-05-07 Qinetiq Ltd Improvements in and relating to the analysis of compounds
US7795039B2 (en) * 2003-06-10 2010-09-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Electronic device for communication with living cells
CA2550274A1 (en) 2003-11-12 2005-05-26 Acea Biosciences, Inc. Real time electronic cell sensing systems and applications for cell-based assays
SE0400421D0 (en) * 2004-02-23 2004-02-23 Astrazeneca Ab assay
US20050241940A1 (en) * 2004-05-03 2005-11-03 Wyeth Fast perfusion system and patch clamp technique utilizing an interface chamber system having high throughput and low volume requirements
US7465558B2 (en) * 2004-05-12 2008-12-16 Wyeth Perfusion system and apparatus for automated multi-channel patch-clamp recordings utilizing inside-out whole-cell configuration
WO2006005370A1 (en) * 2004-07-15 2006-01-19 Nano S Biotechnologie Gmbh Substrate for a device for electrophysiological measurements
US7622934B2 (en) * 2004-07-23 2009-11-24 Electronic Bio Sciences, Llc Method and apparatus for sensing a time varying current passing through an ion channel
US8048289B2 (en) * 2004-09-10 2011-11-01 Molecular Devices, Llc Parallel patch clamp system
WO2006138160A2 (en) * 2005-06-16 2006-12-28 The Regents Of The University Of California Amyloid beta protein channel structure and uses thereof indentifying potential drug molecules for neurodegenerative diseases
US8003380B2 (en) * 2006-01-04 2011-08-23 Agency For Science, Technology And Research High throughput cell-based assays fabricated with integrated silicon and cell culture technologies
US8041515B2 (en) 2006-09-20 2011-10-18 Acea Biosciences, Inc. Use of impedance-based cytological profiling to classify cellular response profiles upon exposure to biologically active agents
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US8217665B2 (en) * 2008-11-25 2012-07-10 Wisconsin Alumni Research Foundation Radio-frequency ion channel probe
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DE102014001916B4 (en) * 2014-02-13 2015-12-03 Christoph Methfessel Measuring chamber for biophysical examination of cells
LU92948B1 (en) * 2016-01-15 2017-09-25 Fachhochschule Aachen CellDrum electrode arrangement for measuring mechanical stress
CN110582569B (en) 2017-03-03 2024-04-02 安捷伦科技有限公司 Methods and systems for functional maturation of iPSC and ESC-derived cardiomyocytes
CN110954646B (en) * 2019-12-13 2022-09-02 深圳供电局有限公司 Method and device for evaluating ablation behavior of contact
US11891689B2 (en) 2020-03-03 2024-02-06 Arizona Board Of Regents On Behalf Of Arizona State University Low-capacitance nanopore sensors on insulating substrates
CN116794139B (en) * 2023-08-24 2023-10-31 中国科学院烟台海岸带研究所 Method for rapidly screening plastic degradation microorganisms based on polymer membrane electrode sensing technology

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9146221B2 (en) 2001-03-24 2015-09-29 Aviva Biosciences Corporation High-density ion transport measurement biochip devices and methods
US9399787B2 (en) 2002-12-20 2016-07-26 Acea Biosciences, Inc. Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays

Also Published As

Publication number Publication date
WO2002004943A2 (en) 2002-01-17
AU7189801A (en) 2002-01-21
IL153545A0 (en) 2003-07-06
US20020053915A1 (en) 2002-05-09
EP1311848A2 (en) 2003-05-21
MXPA03000160A (en) 2003-09-22
JP2004514417A (en) 2004-05-20
AU2001271898B2 (en) 2006-10-05
CA2413663A1 (en) 2002-01-17

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