WO2001023610A3 - Polynucleotide sequencing - Google Patents

Polynucleotide sequencing Download PDF

Info

Publication number
WO2001023610A3
WO2001023610A3 PCT/GB2000/003734 GB0003734W WO0123610A3 WO 2001023610 A3 WO2001023610 A3 WO 2001023610A3 GB 0003734 W GB0003734 W GB 0003734W WO 0123610 A3 WO0123610 A3 WO 0123610A3
Authority
WO
WIPO (PCT)
Prior art keywords
base
polymerase
labelled
terminal
sequencing
Prior art date
Application number
PCT/GB2000/003734
Other languages
French (fr)
Other versions
WO2001023610A2 (en
Inventor
Shankar Balasubramanian
Original Assignee
Solexa Ltd
Shankar Balasubramanian
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9923084.9A external-priority patent/GB9923084D0/en
Priority claimed from GB0002216A external-priority patent/GB0002216D0/en
Application filed by Solexa Ltd, Shankar Balasubramanian filed Critical Solexa Ltd
Priority to AU75372/00A priority Critical patent/AU7537200A/en
Priority to EP00964436A priority patent/EP1218543A2/en
Publication of WO2001023610A2 publication Critical patent/WO2001023610A2/en
Publication of WO2001023610A3 publication Critical patent/WO2001023610A3/en
Priority to US10/113,221 priority patent/US6833246B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for sequencing polynucleotides involves sequential addition of detectably labelled bases. A labelled base is incorporated by a polymerase onto a nascent strand, and, after detection, the polymerase is induced to replace the terminal labelled base with a corresponding unlabelled base, thereby permitting further sequencing to occur. The exonuclease activity of many polymerase enzymes is used, to remove the terminal base.
PCT/GB2000/003734 1999-09-29 2000-09-29 Polynucleotide sequencing WO2001023610A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU75372/00A AU7537200A (en) 1999-09-29 2000-09-29 Polynucleotide sequencing
EP00964436A EP1218543A2 (en) 1999-09-29 2000-09-29 Polynucleotide sequencing
US10/113,221 US6833246B2 (en) 1999-09-29 2002-03-29 Polynucleotide sequencing

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9923084.9 1999-09-29
GBGB9923084.9A GB9923084D0 (en) 1999-09-29 1999-09-29 Polynucleotide sequencing
GB0002216.0 2000-01-31
GB0002216A GB0002216D0 (en) 2000-01-31 2000-01-31 Polyneucleotide sequencing

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/113,221 Continuation US6833246B2 (en) 1999-09-29 2002-03-29 Polynucleotide sequencing

Publications (2)

Publication Number Publication Date
WO2001023610A2 WO2001023610A2 (en) 2001-04-05
WO2001023610A3 true WO2001023610A3 (en) 2001-10-11

Family

ID=26243527

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003734 WO2001023610A2 (en) 1999-09-29 2000-09-29 Polynucleotide sequencing

Country Status (4)

Country Link
US (1) US6833246B2 (en)
EP (1) EP1218543A2 (en)
AU (1) AU7537200A (en)
WO (1) WO2001023610A2 (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9096898B2 (en) 1998-05-01 2015-08-04 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US9121060B2 (en) 2002-08-23 2015-09-01 Illumina Cambridge Limited Modified nucleotides
US9121062B2 (en) 2001-12-04 2015-09-01 Illumina Cambridge Limited Labelled nucleotides
US9127314B2 (en) 2002-08-23 2015-09-08 Illumina Cambridge Limited Labelled nucleotides
US9133511B2 (en) 2000-10-06 2015-09-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US9175342B2 (en) 2007-10-19 2015-11-03 The Trustees Of Columbia University In The City Of New York Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9469873B2 (en) 2006-09-28 2016-10-18 Illumina, Inc. Compositions and methods for nucleotide sequencing
US9528151B2 (en) 2006-12-01 2016-12-27 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators

Families Citing this family (534)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW307746B (en) * 1994-10-14 1997-06-11 Sumitomo Chemical Co
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
EP2100971A3 (en) 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Real-time sequence determination
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
DE60222628T2 (en) * 2001-01-30 2008-07-17 Solexa Ltd., Saffron Walden PREPARATION OF MATRICES FROM POLYNUCLEOTIDES
EP2465943A3 (en) 2001-03-16 2012-10-03 Kalim Mir Linear polymer display
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
WO2004055160A2 (en) * 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
US20040126765A1 (en) * 2002-12-27 2004-07-01 Adams Craig W. Method and compositions for sequencing nucleic acid molecules
JP4691014B2 (en) * 2003-02-26 2011-06-01 カリダ ゲノミクス，インコーポレーテッド Random array DNA analysis by hybridization
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US8637650B2 (en) 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ATE463584T1 (en) 2004-02-19 2010-04-15 Helicos Biosciences Corp METHOD FOR ANALYZING POLYNUCLEOTIDE SEQUENCES
US20100216153A1 (en) * 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20100216151A1 (en) * 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
EP1730307A4 (en) * 2004-03-03 2008-11-05 Univ Columbia Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
WO2006073436A2 (en) * 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
US7833709B2 (en) 2004-05-28 2010-11-16 Wafergen, Inc. Thermo-controllable chips for multiplex analyses
US20070248958A1 (en) 2004-09-15 2007-10-25 Microchip Biotechnologies, Inc. Microfluidic devices
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
EP3492602A1 (en) 2005-06-15 2019-06-05 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
WO2007002204A2 (en) * 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP1951900B1 (en) 2005-10-07 2016-06-15 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
AU2006309096B2 (en) 2005-10-28 2013-07-04 Glaxosmithkline Llc Methods for identifying compounds of interest using encoded libraries
EP1957983A4 (en) * 2005-11-21 2010-03-24 Univ Columbia Multiplex digital immuno-sensing using a library of photocleavable mass tags
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
CA2641271A1 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
CA2643700A1 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
US20090253581A1 (en) 2006-04-04 2009-10-08 Keygene N.V. High Throughput Detection of Molecular Markers Based on AFLP and High Throughput Sequencing
US20080070792A1 (en) * 2006-06-14 2008-03-20 Roland Stoughton Use of highly parallel snp genotyping for fetal diagnosis
EP2366801B1 (en) 2006-06-14 2016-10-19 Verinata Health, Inc Methods for the diagnosis of fetal abnormalities
US8137912B2 (en) * 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
DK2038425T3 (en) 2006-07-12 2010-12-06 Keygene Nv High capacity physical mapping using AFLP
EP2057465A4 (en) 2006-08-09 2010-04-21 Homestead Clinical Corp Organ-specific proteins and methods of their use
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US9201063B2 (en) * 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
US7741045B2 (en) * 2006-11-16 2010-06-22 General Electric Company Sequential analysis of biological samples
US7629125B2 (en) 2006-11-16 2009-12-08 General Electric Company Sequential analysis of biological samples
US7893227B2 (en) * 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US7897737B2 (en) * 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
KR20100028526A (en) 2007-02-05 2010-03-12 마이크로칩 바이오테크놀로지스, 인크. Microfluidic and nanofluidic devices, systems, and applications
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US8222040B2 (en) * 2007-08-28 2012-07-17 Lightspeed Genomics, Inc. Nucleic acid sequencing by selective excitation of microparticles
US8759077B2 (en) * 2007-08-28 2014-06-24 Lightspeed Genomics, Inc. Apparatus for selective excitation of microparticles
NZ584963A (en) 2007-10-02 2012-11-30 Theranos Inc Modular Point-of-care devices as addressible assay units with tips of assay units having interior to immobilize reagents by capillary action
WO2009052214A2 (en) 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US7767441B2 (en) * 2007-10-25 2010-08-03 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US7811810B2 (en) 2007-10-25 2010-10-12 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8518640B2 (en) 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US7897344B2 (en) 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
CN105349647B (en) 2007-10-30 2020-08-28 完整基因有限公司 Methods for high throughput sequencing of nucleic acids
WO2009061840A1 (en) 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN103290106B (en) 2007-12-05 2015-07-29 考利达基因组股份有限公司 In sequencing reaction, base effectively determines
US8101353B2 (en) * 2007-12-18 2012-01-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
US20090181390A1 (en) * 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
GB0804024D0 (en) * 2008-03-04 2008-04-09 Mir Kalim U Multiplex selection
NZ588112A (en) 2008-03-17 2013-04-26 Expressive Res Bv Expression-linked gene discovery
CA2991818C (en) 2008-03-28 2022-10-11 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US20090247426A1 (en) * 2008-03-31 2009-10-01 Pacific Biosciences Of California, Inc. Focused library generation
CN102264917B (en) 2008-06-11 2015-06-10 激光基因公司 Nucleotides and nucleosides and methods for their use in DNA sequencing
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US8795961B2 (en) * 2008-09-05 2014-08-05 Pacific Biosciences Of California, Inc. Preparations, compositions, and methods for nucleic acid sequencing
EP2384429A1 (en) 2008-12-31 2011-11-09 Integenx Inc. Instrument with microfluidic chip
EP2379751B1 (en) 2009-01-13 2013-03-20 Keygene N.V. Novel genome sequencing strategies
EP2393944A4 (en) * 2009-02-03 2013-11-27 Complete Genomics Inc Indexing a reference sequence for oligomer sequence mapping
EP2394165A4 (en) * 2009-02-03 2013-12-11 Complete Genomics Inc Oligomer sequences mapping
US8615365B2 (en) * 2009-02-03 2013-12-24 Complete Genomics, Inc. Oligomer sequences mapping
EP2430441B1 (en) * 2009-04-29 2018-06-13 Complete Genomics, Inc. Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
EP2425240A4 (en) 2009-04-30 2012-12-12 Good Start Genetics Inc Methods and compositions for evaluating genetic markers
CN102459565A (en) 2009-06-02 2012-05-16 尹特根埃克斯有限公司 Fluidic devices with diaphragm valves
US8632975B2 (en) * 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
RU2559541C2 (en) 2009-06-05 2015-08-10 Интедженкс Инк. Universal system for sample preparing and application in integrated system of analysis
WO2010148039A2 (en) 2009-06-15 2010-12-23 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
US20110040081A1 (en) * 2009-08-14 2011-02-17 Epicentre Technologies Corporation METHODS, COMPOSITIONS, AND KITS FOR GENERATING rRNA-DEPLETED SAMPLES OR ISOLATING rRNA FROM SAMPLES
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
WO2011047307A1 (en) 2009-10-15 2011-04-21 Ibis Biosciences, Inc. Multiple displacement amplification
US9677125B2 (en) * 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
EP2496720B1 (en) 2009-11-06 2020-07-08 The Board of Trustees of the Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8502867B2 (en) 2010-03-19 2013-08-06 Lightspeed Genomics, Inc. Synthetic aperture optics imaging method using minimum selective excitation patterns
US9465228B2 (en) 2010-03-19 2016-10-11 Optical Biosystems, Inc. Illumination apparatus optimized for synthetic aperture optics imaging using minimum selective excitation patterns
PL2556171T3 (en) 2010-04-05 2016-04-29 Prognosys Biosciences Inc Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
SI2558577T1 (en) 2010-04-16 2019-05-31 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
US8774494B2 (en) 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
SG10201503540QA (en) 2010-05-06 2015-06-29 Ibis Biosciences Inc Integrated sample preparation systems and stabilized enzyme mixtures
US10240194B2 (en) 2010-05-13 2019-03-26 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US20120035062A1 (en) 2010-06-11 2012-02-09 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
EP2606154B1 (en) 2010-08-20 2019-09-25 Integenx Inc. Integrated analysis system
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US9880089B2 (en) 2010-08-31 2018-01-30 Complete Genomics, Inc. High-density devices with synchronous tracks for quad-cell based alignment correction
US9671344B2 (en) 2010-08-31 2017-06-06 Complete Genomics, Inc. High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging
EP2614159B1 (en) 2010-09-10 2017-11-08 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
AU2011301935B2 (en) 2010-09-16 2015-06-11 Ibis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
GB2499340B (en) 2010-10-04 2015-10-28 Genapsys Inc Methods for sequencing nucleic acids
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
EP3141614B1 (en) 2010-10-27 2018-11-28 Life Technologies Corporation Predictive model for use in sequencing-by-synthesis
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
EP3709303A1 (en) 2010-12-14 2020-09-16 Life Technologies Corporation Systems and methods for run-time sequencing run quality monitoring
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
CN105755545B (en) 2010-12-27 2019-05-03 艾比斯生物科学公司 The preparation method and composition of nucleic acid samples
WO2012118555A1 (en) 2010-12-29 2012-09-07 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
WO2012092455A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
WO2012092515A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US9217132B2 (en) 2011-01-20 2015-12-22 Ibis Biosciences, Inc. Microfluidic transducer
TW202208825A (en) 2011-01-21 2022-03-01 美商拉布拉多診斷有限責任公司 Systems and methods for sample use maximization
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US20120190021A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US10144950B2 (en) 2011-01-31 2018-12-04 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
US20120219950A1 (en) 2011-02-28 2012-08-30 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
WO2012138921A1 (en) 2011-04-08 2012-10-11 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
WO2012139110A2 (en) 2011-04-08 2012-10-11 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
WO2012177792A2 (en) 2011-06-24 2012-12-27 Sequenom, Inc. Methods and processes for non-invasive assessment of a genetic variation
JP6028025B2 (en) 2011-07-08 2016-11-16 キージーン・エン・フェー Sequence-based genotyping based on oligonucleotide ligation assays
US9670538B2 (en) 2011-08-05 2017-06-06 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
EP2745108B1 (en) 2011-08-18 2019-06-26 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
CN104024269B (en) 2011-09-13 2017-05-24 激光基因公司 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
EP2768972B2 (en) 2011-09-23 2020-07-22 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US20140242588A1 (en) 2011-10-06 2014-08-28 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
GB2497838A (en) 2011-10-19 2013-06-26 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
WO2013063308A1 (en) 2011-10-25 2013-05-02 University Of Massachusetts An enzymatic method to enrich for capped rna, kits for performing same, and compositions derived therefrom
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
CN104094266A (en) 2011-11-07 2014-10-08 独创***公司 Methods and systems for identification of causal genomic variants
JP6193252B2 (en) 2011-12-01 2017-09-06 ジナプシス インコーポレイテッド System and method for high efficiency electronic sequencing and detection
WO2013096460A1 (en) 2011-12-20 2013-06-27 The Regents Of The University Of Michigan Pseudogenes and uses thereof
ES2645418T3 (en) 2011-12-22 2017-12-05 Ibis Biosciences, Inc. Amplification of a sequence of a ribonucleic acid
ES2626058T3 (en) 2011-12-22 2017-07-21 Ibis Biosciences, Inc. Primers and amplification methods
WO2013096799A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
US10150993B2 (en) 2011-12-22 2018-12-11 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
US9803188B2 (en) 2011-12-22 2017-10-31 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
US9506113B2 (en) 2011-12-28 2016-11-29 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
US9803231B2 (en) 2011-12-29 2017-10-31 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
CA2863121A1 (en) 2011-12-30 2013-07-04 Abbott Molecular Inc. Microorganism nucleic acid purification from host samples
JP2015503923A (en) 2012-01-09 2015-02-05 オスロ ウニヴェルスィテーツスィーケフース ハーエフＯｓｌｏ Ｕｎｉｖｅｒｓｉｔｅｔｓｓｙｋｅｈｕｓ Ｈｆ Methods and biomarkers for the analysis of colorectal cancer
US20130184165A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
EP2805280B1 (en) 2012-01-20 2022-10-05 Sequenom, Inc. Diagnostic processes that factor experimental conditions
CN105861487B (en) 2012-01-26 2020-05-05 纽亘技术公司 Compositions and methods for targeted nucleic acid sequence enrichment and efficient library generation
US9515676B2 (en) 2012-01-31 2016-12-06 Life Technologies Corporation Methods and computer program products for compression of sequencing data
US9864846B2 (en) 2012-01-31 2018-01-09 Life Technologies Corporation Methods and computer program products for compression of sequencing data
PT3363901T (en) 2012-02-17 2021-01-22 Hutchinson Fred Cancer Res Compositions and methods for accurately identifying mutations
WO2013124738A2 (en) 2012-02-21 2013-08-29 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
AU2013229151A1 (en) 2012-03-06 2014-09-25 Aarhus University Gene signatures associated with efficacy of postmastectomy radiotherapy in breast cancer
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
ES2716995T3 (en) 2012-04-03 2019-06-18 Univ Michigan Regents Biomarkers associated with irritable bowel syndrome and Crohn's disease
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
ES2833524T3 (en) 2012-05-02 2021-06-15 Ibis Biosciences Inc DNA sequencing
EP2844772B1 (en) 2012-05-02 2018-07-11 Ibis Biosciences, Inc. Dna sequencing
EP2844774B1 (en) 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna sequencing
WO2013166302A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
EP2844771A4 (en) 2012-05-04 2015-12-02 Complete Genomics Inc Methods for determining absolute genome-wide copy number variations of complex tumors
ES2905448T3 (en) 2012-05-10 2022-04-08 Massachusetts Gen Hospital Methods for determining a nucleotide sequence
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
ES2928691T3 (en) 2012-05-21 2022-11-22 Scripps Research Inst Sample preparation methods
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
EP2852608A4 (en) 2012-05-21 2016-05-04 Distributed Bio Inc Epitope focusing by variable effective antigen surface concentration
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
US9267168B2 (en) 2012-06-12 2016-02-23 Pacific Biosciences Of California, Inc. Methods and compositions for isolating template nucleic acids
CN104619894B (en) 2012-06-18 2017-06-06 纽亘技术公司 For the composition and method of the Solid phase of unexpected nucleotide sequence
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
JP6285929B2 (en) 2012-07-17 2018-02-28 カウンシル，インコーポレーテッド System and method for detecting genetic variation
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
US9206417B2 (en) 2012-07-19 2015-12-08 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
WO2014026032A2 (en) 2012-08-08 2014-02-13 Apprise Bio, Inc. Increasing dynamic range for identifying multiple epitopes in cells
RS56093B1 (en) 2012-09-19 2017-10-31 Abbvie Biotherapeutics Inc Methods for identifying antibodies with reduced immunogenicity
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
ES2701750T3 (en) 2012-10-16 2019-02-25 Abbott Molecular Inc Procedures for sequencing a nucleic acid
WO2014074611A1 (en) 2012-11-07 2014-05-15 Good Start Genetics, Inc. Methods and systems for identifying contamination in samples
WO2014108850A2 (en) 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2899020C (en) 2013-02-01 2023-10-03 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
CA2902099C (en) 2013-03-08 2020-06-02 F. Hoffmann-La Roche Ag Egfr mutation blood testing
WO2014160233A1 (en) 2013-03-13 2014-10-02 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
US9701999B2 (en) 2013-03-14 2017-07-11 Abbott Molecular, Inc. Multiplex methylation-specific amplification systems and methods
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
EP2971154A4 (en) 2013-03-14 2017-03-01 Ibis Biosciences, Inc. Nucleic acid control panels
EP2971159B1 (en) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Methods for analyzing nucleic acids
BR112015022448B1 (en) 2013-03-15 2020-12-08 Illumina Cambridge Limited modified nucleotide or nucleoside molecule, methods for preparing the growth of polynucleotide complementary to single-stranded target polynucleotide in sequencing reaction and to determine the sequence of single-stranded target polynucleotide and kit
CN105051214B (en) 2013-03-15 2018-12-28 吉纳普赛斯股份有限公司 System and method for bioanalysis
ES2716094T3 (en) 2013-03-15 2019-06-10 Ibis Biosciences Inc Methods to analyze contamination in DNA sequencing
WO2014150851A1 (en) 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
EP3415626B1 (en) 2013-03-15 2020-01-22 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples
CN113337604A (en) 2013-03-15 2021-09-03 莱兰斯坦福初级大学评议会 Identification and use of circulating nucleic acid tumor markers
EP2971139A4 (en) 2013-03-15 2016-12-07 Abbott Molecular Inc Systems and methods for detection of genomic copy number changes
EP4187543A1 (en) 2013-04-03 2023-05-31 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN105555968B (en) 2013-05-24 2020-10-23 塞昆纳姆股份有限公司 Methods and processes for non-invasive assessment of genetic variation
EP3005200A2 (en) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Methods and systems for storing sequence read data
JP6426162B2 (en) 2013-06-13 2018-11-21 アリオサ ダイアグノスティックス インコーポレイテッドＡｒｉｏｓａ Ｄｉａｇｎｏｓｔｉｃｓ，Ｉｎｃ． Method of noninvasively calculating the risk of fetal sex chromosome aneuploidy
IL283586B2 (en) 2013-06-21 2023-11-01 Sequenom Inc Methods and processes for non-invasive assessment of genetic variations
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
CN105531377A (en) 2013-07-30 2016-04-27 哈佛学院院长及董事 Quantitative dna-based imaging and super-resolution imaging
CN105637097A (en) 2013-08-05 2016-06-01 特韦斯特生物科学公司 De novo synthesized gene libraries
US10036013B2 (en) 2013-08-19 2018-07-31 Abbott Molecular Inc. Next-generation sequencing libraries
IL285521B2 (en) 2013-08-19 2023-03-01 Singular Bio Inc Assays for single molecule detection
US9116866B2 (en) 2013-08-21 2015-08-25 Seven Bridges Genomics Inc. Methods and systems for detecting sequence variants
US9898575B2 (en) 2013-08-21 2018-02-20 Seven Bridges Genomics Inc. Methods and systems for aligning sequences
WO2015027245A1 (en) 2013-08-23 2015-02-26 Complete Genomics, Inc. Long fragment de novo assembly using short reads
US10351899B2 (en) 2013-09-25 2019-07-16 Bio-ID Diagnostics Inc. Methods for detecting nucleic acid fragments
JP6532456B2 (en) 2013-10-04 2019-06-19 ライフ テクノロジーズ コーポレーション Methods and systems for modeling PHASING EFFECTS in sequencing using termination chemistry
ES2968644T3 (en) 2013-10-04 2024-05-13 Sequenom Inc Methods and procedures for the non-invasive evaluation of genetic variations
EP3495496B1 (en) 2013-10-07 2020-11-25 Sequenom, Inc. Methods and processes for non-invasive assessment of chromosome alterations
EP3875601A1 (en) 2013-10-17 2021-09-08 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
EP3058096A1 (en) 2013-10-18 2016-08-24 Good Start Genetics, Inc. Methods for assessing a genomic region of a subject
WO2015058120A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for aligning sequences in the presence of repeating elements
WO2015058095A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for quantifying sequence alignment
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
SG11201602903XA (en) 2013-10-18 2016-05-30 Seven Bridges Genomics Inc Methods and systems for genotyping genetic samples
SG11201603039PA (en) 2013-10-18 2016-05-30 Seven Bridges Genomics Inc Methods and systems for identifying disease-induced mutations
US9092402B2 (en) 2013-10-21 2015-07-28 Seven Bridges Genomics Inc. Systems and methods for using paired-end data in directed acyclic structure
KR102649364B1 (en) 2013-11-07 2024-03-20 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 Cell-free nucleic acids for the analysis of the human microbiome and components thereof
CN105849264B (en) 2013-11-13 2019-09-27 纽亘技术公司 For identifying the composition and method that repeat sequencing reading
US9546398B2 (en) * 2013-11-14 2017-01-17 Agilent Technologies, Inc. Polymerase idling method for single molecule DNA sequencing
WO2015073999A1 (en) 2013-11-18 2015-05-21 Integenx Inc. Cartridges and instruments for sample analysis
EP3080268A1 (en) 2013-12-09 2016-10-19 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
AU2014362322B2 (en) 2013-12-11 2021-05-27 The Regents For Of The University Of California Methods for labeling DNA fragments to recontruct physical linkage and phase
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3792921A1 (en) 2013-12-11 2021-03-17 Genapsys, Inc. Systems and methods for biological analysis and computation
US10767222B2 (en) 2013-12-11 2020-09-08 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US9909181B2 (en) 2013-12-13 2018-03-06 Northwestern University Biomarkers for post-traumatic stress states
EP3083700B1 (en) 2013-12-17 2023-10-11 The Brigham and Women's Hospital, Inc. Detection of an antibody against a pathogen
JP2017510871A (en) 2014-01-10 2017-04-13 セブン ブリッジズ ジェノミクス インコーポレイテッド System and method for use of known alleles in read mapping
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
AU2015209079B2 (en) * 2014-01-27 2021-07-15 Archerdx, Llc Methods of preparing nucleic acids for sequencing
US9817944B2 (en) 2014-02-11 2017-11-14 Seven Bridges Genomics Inc. Systems and methods for analyzing sequence data
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
AU2015229406C1 (en) 2014-03-11 2021-01-07 President And Fellows Of Harvard College High-throughput and highly multiplexed imaging with programmable nucleic acid probes
WO2015161054A2 (en) 2014-04-18 2015-10-22 Genapsys, Inc. Methods and systems for nucleic acid amplification
US11053548B2 (en) 2014-05-12 2021-07-06 Good Start Genetics, Inc. Methods for detecting aneuploidy
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
EP3151733B1 (en) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
AU2015280069B2 (en) 2014-06-23 2021-08-12 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
CN114214314A (en) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 Digital PCR barcoding
ES2753573T3 (en) 2014-06-27 2020-04-13 Abbott Lab Compositions and methods for detecting human pegivirus 2 (HPGV-2)
CA2955771C (en) 2014-07-24 2022-08-30 Abbott Molecular Inc. Compositions and methods for the detection and analysis of mycobacterium tuberculosis
US20160034640A1 (en) 2014-07-30 2016-02-04 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP4219710A3 (en) 2014-08-01 2023-08-16 Dovetail Genomics, LLC Tagging nucleic acids for sequence assembly
EP3913066A1 (en) 2014-09-09 2021-11-24 Igenomx International Genomics Corporation Compositions for rapid nucleic acid library preparation
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
WO2016044391A1 (en) 2014-09-17 2016-03-24 Ibis Biosciences, Inc. Sequencing by synthesis using pulse read optics
EP3224595A4 (en) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
CN107408043A (en) 2014-10-14 2017-11-28 七桥基因公司 System and method for the intelligence tool in sequence streamline
EP3835429A1 (en) 2014-10-17 2021-06-16 Good Start Genetics, Inc. Pre-implantation genetic screening and aneuploidy detection
CN113092563B (en) 2014-10-22 2024-06-07 尹特根埃克斯有限公司 Systems and methods for sample preparation, processing, and analysis
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
CA3010579A1 (en) 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016134034A1 (en) 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
CN107250383B (en) 2015-02-17 2022-09-06 深圳华大智造科技股份有限公司 DNA sequencing using controlled strand displacement
WO2016134191A1 (en) 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
WO2016134342A1 (en) 2015-02-20 2016-08-25 Wafergen, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
US10192026B2 (en) 2015-03-05 2019-01-29 Seven Bridges Genomics Inc. Systems and methods for genomic pattern analysis
WO2016154540A1 (en) 2015-03-26 2016-09-29 Dovetail Genomics Llc Physical linkage preservation in dna storage
ES2955916T3 (en) 2015-04-10 2023-12-11 Spatial Transcriptomics Ab Multiplex analysis of biological specimens of spatially distinguished nucleic acids
EP3283627B1 (en) 2015-04-15 2021-12-01 The General Hospital Corporation Lna-based mutant enrichment next-generation sequencing assays
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10978174B2 (en) 2015-05-14 2021-04-13 Life Technologies Corporation Barcode sequences, and related systems and methods
US10275567B2 (en) 2015-05-22 2019-04-30 Seven Bridges Genomics Inc. Systems and methods for haplotyping
EP3322805B1 (en) 2015-07-14 2021-10-20 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides or magnesium-titanium oxides
CA2992578A1 (en) 2015-07-14 2017-01-19 Victor Velculescu Neoantigen analysis
EP3322483A4 (en) 2015-07-14 2019-01-02 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
US10465245B2 (en) 2015-07-29 2019-11-05 Progenity, Inc. Nucleic acids and methods for detecting chromosomal abnormalities
WO2017027370A1 (en) 2015-08-07 2017-02-16 President And Fellows Of Harvard College Super resolution imaging of protein-protein interactions
US10793895B2 (en) 2015-08-24 2020-10-06 Seven Bridges Genomics Inc. Systems and methods for epigenetic analysis
US10584380B2 (en) 2015-09-01 2020-03-10 Seven Bridges Genomics Inc. Systems and methods for mitochondrial analysis
US10724110B2 (en) 2015-09-01 2020-07-28 Seven Bridges Genomics Inc. Systems and methods for analyzing viral nucleic acids
AU2016319110B2 (en) 2015-09-11 2022-01-27 The General Hospital Corporation Full interrogation of nuclease DSBs and sequencing (FIND-seq)
US10844373B2 (en) 2015-09-18 2020-11-24 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (en) 2015-09-22 2018-10-23 特韦斯特生物科学公司 Flexible substrates for nucleic acid synthesis
JP2018529353A (en) 2015-09-30 2018-10-11 ザ ジェネラル ホスピタル コーポレイション Comprehensive in vitro reporting of cleavage events by sequencing (CIRCLE-seq)
US10465232B1 (en) 2015-10-08 2019-11-05 Trace Genomics, Inc. Methods for quantifying efficiency of nucleic acid extraction and detection
CA2999902A1 (en) 2015-10-09 2017-04-13 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
ES2796501T3 (en) 2015-10-10 2020-11-27 Guardant Health Inc Methods and applications of gene fusion detection in cell-free DNA analysis
US11347704B2 (en) 2015-10-16 2022-05-31 Seven Bridges Genomics Inc. Biological graph or sequence serialization
EP3365445B1 (en) 2015-10-19 2023-05-31 Dovetail Genomics, LLC Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
US11421264B2 (en) 2015-11-06 2022-08-23 IsoPlexis Corporation Thiol-containing cleave reagents and oxidative wash
KR102454782B1 (en) 2015-11-06 2022-10-13 퀴아젠 사이언시스, 엘엘씨 Nucleotide analogues
CA3005101A1 (en) 2015-11-16 2017-05-26 Progenity, Inc. Nucleic acids and methods for detecting methylation status
CN115920796A (en) 2015-12-01 2023-04-07 特韦斯特生物科学公司 Functionalized surfaces and preparation thereof
CN108699505A (en) 2015-12-03 2018-10-23 安可济控股有限公司 It is used to form the method and composition of connection product
EP3390668A4 (en) 2015-12-17 2020-04-01 Guardant Health, Inc. Methods to determine tumor gene copy number by analysis of cell-free dna
US20170199960A1 (en) 2016-01-07 2017-07-13 Seven Bridges Genomics Inc. Systems and methods for adaptive local alignment for graph genomes
CN108431223A (en) 2016-01-08 2018-08-21 生物辐射实验室股份有限公司 Multiple pearls under per drop resolution
US10364468B2 (en) 2016-01-13 2019-07-30 Seven Bridges Genomics Inc. Systems and methods for analyzing circulating tumor DNA
US10460829B2 (en) 2016-01-26 2019-10-29 Seven Bridges Genomics Inc. Systems and methods for encoding genetic variation for a population
CN108779442B (en) 2016-02-08 2022-07-12 瑞尔基因公司 Compositions, systems, and methods for multiple ligases
EP3420108A4 (en) 2016-02-23 2019-11-06 Dovetail Genomics LLC Generation of phased read-sets for genome assembly and haplotype phasing
US10262102B2 (en) 2016-02-24 2019-04-16 Seven Bridges Genomics Inc. Systems and methods for genotyping with graph reference
US20190062827A1 (en) 2016-03-14 2019-02-28 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
KR102326769B1 (en) 2016-03-25 2021-11-17 카리우스, 인코포레이티드 Synthetic nucleic acid spike-ins
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
US11021742B2 (en) 2016-03-28 2021-06-01 Boreal Genomics, Inc. Linked-fragment sequencing
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
AU2017263810B2 (en) 2016-05-13 2023-08-17 Dovetail Genomics Llc Recovering long-range linkage information from preserved samples
EP3458586B1 (en) 2016-05-16 2022-12-28 Accuragen Holdings Limited Method of improved sequencing by strand identification
US11118233B2 (en) 2016-05-18 2021-09-14 The University Of Chicago BTK mutation and ibrutinib resistance
CA3026193A1 (en) 2016-06-06 2017-12-14 Redvault Biosciences, Lp Target reporter constructs and uses thereof
WO2018013509A1 (en) 2016-07-11 2018-01-18 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
WO2018013558A1 (en) 2016-07-12 2018-01-18 Life Technologies Corporation Compositions and methods for detecting nucleic acid regions
CN116397014A (en) 2016-07-20 2023-07-07 测序健康公司 Systems and methods for nucleic acid sequencing
WO2018017892A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
WO2018022890A1 (en) 2016-07-27 2018-02-01 Sequenom, Inc. Genetic copy number alteration classifications
CA3033749A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
WO2018042251A1 (en) 2016-08-29 2018-03-08 Oslo Universitetssykehus Hf Chip-seq assays
US11250931B2 (en) 2016-09-01 2022-02-15 Seven Bridges Genomics Inc. Systems and methods for detecting recombination
KR102217487B1 (en) 2016-09-21 2021-02-23 트위스트 바이오사이언스 코포레이션 Nucleic acid-based data storage
WO2018064311A2 (en) 2016-09-28 2018-04-05 Life Technologies Corporation Methods and systems for reducing phasing errors when sequencing nucleic acids using termination chemistry
TWI826089B (en) 2016-11-03 2023-12-11 大陸商深圳華大智造科技有限公司 Biosensors for biological or chemical analysis and methods of manufacturing the same
CN110023509A (en) 2016-11-15 2019-07-16 私人基因诊断公司 Non- unique bar code in genotyping measurement
CN110050071A (en) 2016-12-09 2019-07-23 乌尔蒂维尤股份有限公司 The improved method that nucleic acid preparation for using label carries out multiplexing imaging
CA3048420A1 (en) 2016-12-09 2018-06-14 Boreal Genomics, Inc. Linked ligation
KR102514213B1 (en) 2016-12-16 2023-03-27 트위스트 바이오사이언스 코포레이션 Immune synaptic variant library and its synthesis
EP3555290B1 (en) 2016-12-19 2022-11-02 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
EP3565905B1 (en) 2017-01-04 2022-05-25 MGI Tech Co., Ltd. Nucleic acid sequencing using affinity reagents
EP3574424A1 (en) 2017-01-24 2019-12-04 Sequenom, Inc. Methods and processes for assessment of genetic variations
DK3354746T3 (en) 2017-01-30 2019-09-02 Gmi Gregor Mendel Inst Fuer Molekulare Pflanzenbiologie Gmbh NEW SPIKE-IN NUCLEOTIDES FOR NORMALIZING SEQUENCE DATA
SG11201907713WA (en) 2017-02-22 2019-09-27 Twist Bioscience Corp Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN110506336B (en) 2017-03-20 2024-02-02 深圳华大智造科技股份有限公司 Biosensor for biological or chemical analysis and method for manufacturing the same
EP3601593B1 (en) 2017-03-24 2021-12-22 Bio-Rad Laboratories, Inc. Universal hairpin primers
WO2018183942A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
CA3059370C (en) 2017-04-12 2022-05-10 Karius, Inc. Methods for concurrent analysis of dna and rna in mixed samples
US20210371918A1 (en) 2017-04-18 2021-12-02 Dovetail Genomics, Llc Nucleic acid characteristics as guides for sequence assembly
EP3634992B1 (en) 2017-06-08 2024-03-27 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
SG11201912057RA (en) 2017-06-12 2020-01-30 Twist Bioscience Corp Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018236918A1 (en) 2017-06-20 2018-12-27 Bio-Rad Laboratories, Inc. Mda using bead oligonucleotide
CA3068273A1 (en) 2017-06-21 2018-12-27 Bluedot Llc Systems and methods for identification of nucleic acids in a sample
WO2019002265A1 (en) 2017-06-26 2019-01-03 Universität Für Bodenkultur Wien Novel biomarkers for detecting senescent cells
WO2019051501A1 (en) 2017-09-11 2019-03-14 Twist Bioscience Corporation Gpcr binding proteins and synthesis thereof
EP3685426A4 (en) 2017-09-19 2021-06-09 MGI Tech Co., Ltd. Wafer level sequencing flow cell fabrication
WO2019060640A1 (en) 2017-09-20 2019-03-28 Guardant Health, Inc. Methods and systems for differentiating somatic and germline variants
JP2021500858A (en) 2017-09-21 2021-01-14 ジナプシス インコーポレイテッド Systems and methods for nucleic acid sequencing
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. Methods and systems for sample extraction
CA3077085A1 (en) 2017-10-06 2019-04-11 Oncotherapy Science, Inc. Screening of t lymphocytes for cancer-specific antigens
JP7066840B2 (en) 2017-10-20 2022-05-13 ツイスト バイオサイエンス コーポレーション Heated nanowells for polynucleotide synthesis
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN118186055A (en) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 Transposase-based genomic analysis
AU2018372906A1 (en) 2017-11-22 2020-06-11 The Regents Of The University Of Michigan Compositions and methods for treating cancer
JP2021509024A (en) 2018-01-02 2021-03-18 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン Capturing a large number of droplets
EP3735459A4 (en) 2018-01-04 2021-10-06 Twist Bioscience Corporation Dna-based digital information storage
US20190237163A1 (en) 2018-01-12 2019-08-01 Life Technologies Corporation Methods for flow space quality score prediction by neural networks
US10844430B2 (en) 2018-01-24 2020-11-24 Qiagen Sciences, Llc DNA sequencing reaction additive
WO2019152539A1 (en) 2018-01-30 2019-08-08 Optical Biosystems, Inc. Method for detecting particles using structured illumination
CA3090102A1 (en) 2018-01-31 2019-08-08 Dovetail Genomics, Llc Sample prep for dna linkage recovery
WO2019152395A1 (en) 2018-01-31 2019-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
US20210239700A1 (en) 2018-05-04 2021-08-05 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
KR20210013128A (en) 2018-05-18 2021-02-03 트위스트 바이오사이언스 코포레이션 Polynucleotides, reagents and methods for nucleic acid hybridization
WO2019236478A1 (en) 2018-06-04 2019-12-12 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free nucleic acids
EP3827100A2 (en) 2018-07-23 2021-06-02 Guardant Health, Inc. Methods and systems for adjusting tumor mutational burden by tumor fraction and coverage
CN113166807B (en) 2018-08-20 2024-06-04 生物辐射实验室股份有限公司 Nucleotide sequence generation by co-localization of barcode beads in partitions
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
KR20210052501A (en) 2018-08-30 2021-05-10 가던트 헬쓰, 인크. Methods and systems for detecting contamination between samples
AU2019328344A1 (en) 2018-08-31 2021-04-08 Guardant Health, Inc. Microsatellite instability detection in cell-free DNA
JP2021534803A (en) 2018-09-04 2021-12-16 ガーダント ヘルス， インコーポレイテッド Methods and systems for detecting allelic imbalances in cell-free nucleic acid samples
SG11202101798QA (en) 2018-09-20 2021-04-29 Tamirna Gmbh Micro-rna signatures for the prediction of liver dysfunction
US20200131566A1 (en) 2018-10-31 2020-04-30 Guardant Health, Inc. Methods, compositions and systems for calibrating epigenetic partitioning assays
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
TW202043450A (en) 2018-11-15 2020-12-01 中國商深圳華大智造科技有限公司 System and method for integrated sensor cartridge
EP3887802B1 (en) 2018-12-01 2024-05-01 MGI Tech Co., Ltd. Biosensor with improved light collection efficiency
CN112368077B (en) 2018-12-07 2023-11-24 元素生物科学公司 Flow cell device and use thereof
JP2022514010A (en) 2018-12-20 2022-02-09 ガーダント ヘルス， インコーポレイテッド Methods, compositions, and systems for improving the recovery of nucleic acid molecules
US11473136B2 (en) 2019-01-03 2022-10-18 Ncan Genomics, Inc. Linked target capture
US20220081714A1 (en) 2019-01-04 2022-03-17 Northwestern University Storing temporal data into dna
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3908823A4 (en) 2019-01-10 2022-10-12 Travera LLC Calibration of a functional biomarker instrument
WO2020160414A1 (en) 2019-01-31 2020-08-06 Guardant Health, Inc. Compositions and methods for isolating cell-free dna
CN113766930A (en) 2019-02-26 2021-12-07 特韦斯特生物科学公司 Variant nucleic acid libraries of GLP1 receptors
CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
WO2020176659A1 (en) 2019-02-27 2020-09-03 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free dna
WO2020180813A1 (en) 2019-03-06 2020-09-10 Qiagen Sciences, Llc Compositions and methods for adaptor design and nucleic acid library construction for rolony-based sequencing
WO2020206170A1 (en) 2019-04-02 2020-10-08 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
US11579217B2 (en) 2019-04-12 2023-02-14 Western Digital Technologies, Inc. Devices and methods for frequency- and phase-based detection of magnetically-labeled molecules using spin torque oscillator (STO) sensors
US11327073B2 (en) 2019-04-12 2022-05-10 Western Digital Technologies, Inc. Thermal sensor array for molecule detection and related detection schemes
US11609208B2 (en) 2019-04-12 2023-03-21 Western Digital Technologies, Inc. Devices and methods for molecule detection based on thermal stabilities of magnetic nanoparticles
US11112468B2 (en) 2019-04-12 2021-09-07 Western Digital Technologies, Inc. Magnetoresistive sensor array for molecule detection and related detection schemes
US11738336B2 (en) 2019-04-12 2023-08-29 Western Digital Technologies, Inc. Spin torque oscillator (STO) sensors used in nucleic acid sequencing arrays and detection schemes for nucleic acid sequencing
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11939636B2 (en) 2019-05-31 2024-03-26 Guardant Health, Inc. Methods and systems for improving patient monitoring after surgery
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US11208682B2 (en) 2019-09-13 2021-12-28 Western Digital Technologies, Inc. Enhanced optical detection for nucleic acid sequencing using thermally-dependent fluorophore tags
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US11747329B2 (en) 2019-11-22 2023-09-05 Western Digital Technologies, Inc. Magnetic gradient concentrator/reluctance detector for molecule detection
CA3157560A1 (en) 2019-11-26 2021-06-03 Dustin Howard HITE Methods, compositions and systems for improving the binding of methylated polynucleotides
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
CN115698282A (en) 2020-01-13 2023-02-03 福路伦特生物科学公司 Single cell sequencing
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
EP4103580A4 (en) 2020-02-13 2024-03-06 Zymergen Inc. Metagenomic library and natural product discovery platform
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
CA3175931A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
WO2021185320A1 (en) 2020-03-18 2021-09-23 Mgi Tech Co., Ltd. Restoring phase in massively parallel sequencing
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
ES2965354T3 (en) 2020-04-22 2024-04-12 10X Genomics Inc Methods for spatial analysis using targeted RNA deletion
EP4143338A1 (en) 2020-04-30 2023-03-08 Guardant Health, Inc. Methods for sequence determination using partitioned nucleic acids
US20230203592A1 (en) 2020-05-05 2023-06-29 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US20220025468A1 (en) 2020-05-14 2022-01-27 Guardant Health, Inc. Homologous recombination repair deficiency detection
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
MX2022014931A (en) 2020-05-27 2023-01-30 Genetic Tech Limited Methods of assessing risk of developing a severe response to coronavirus infection.
EP4158054A1 (en) 2020-06-02 2023-04-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
WO2021247543A2 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Nucleic acid library methods
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
CN116034166A (en) 2020-06-25 2023-04-28 10X基因组学有限公司 Spatial analysis of DNA methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
WO2023282916A1 (en) 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
US11946044B2 (en) 2020-07-30 2024-04-02 Guardant Health, Inc. Methods for isolating cell-free DNA
WO2022029484A1 (en) 2020-08-06 2022-02-10 Agendia NV Methods of assessing breast cancer using circulating hormone receptor transcripts
US20220042108A1 (en) 2020-08-06 2022-02-10 Agendia NV Systems and methods of assessing breast cancer
US20220042106A1 (en) 2020-08-06 2022-02-10 Agendia NV Systems and methods of using cell-free nucleic acids to tailor cancer treatment
US20220042107A1 (en) 2020-08-06 2022-02-10 Agendia NV Systems and methods of scoring risk and residual disease from passenger mutations
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
JP2023540221A (en) 2020-08-25 2023-09-22 ガーダント ヘルス， インコーポレイテッド Methods and systems for predicting variant origin
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
JP2023544721A (en) 2020-09-30 2023-10-25 ガーダント ヘルス， インコーポレイテッド Compositions and methods for analyzing DNA using partitioning and methylation-dependent nucleases
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4267757A1 (en) 2020-12-23 2023-11-01 Guardant Health, Inc. Methods and systems for analyzing methylated polynucleotides
WO2022174054A1 (en) 2021-02-13 2022-08-18 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
WO2022187862A1 (en) 2021-03-05 2022-09-09 Guardant Health, Inc. Methods and related aspects for analyzing molecular response
JP2024512372A (en) 2021-03-09 2024-03-19 ガーダント ヘルス， インコーポレイテッド Detection of tumor presence based on off-target polynucleotide sequencing data
EP4301870A1 (en) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023086635A1 (en) 2021-11-15 2023-05-19 Vir Biotechnology, Inc. Virological and molecular surrogates of response to sars-cov-2 neutralizing antibody sotrovimab
WO2023135485A1 (en) 2022-01-13 2023-07-20 Oslo Universitetssykehus Hf Prostate cancer markers and uses thereof
WO2023152568A2 (en) 2022-02-10 2023-08-17 Oslo Universitetssykehus Hf Compositions and methods for characterizing lung cancer
US11639499B1 (en) 2022-03-08 2023-05-02 Watchmaker Genomics, Inc. Reverse transcriptase variants
US20230360725A1 (en) 2022-05-09 2023-11-09 Guardant Health, Inc. Detecting degradation based on strand bias
WO2024107599A1 (en) 2022-11-15 2024-05-23 Guardant Health, Inc. Method of predicting non-small cell lung cancer (nsclc) patient drug response or time until death or cancer progression from circulating tumor dna (ctdna) utilizing signals from both baseline ctdna level and longitudinal change of ctdna level over time
WO2024123733A1 (en) 2022-12-05 2024-06-13 Twist Bioscience Corporation Enzymes for library preparation
WO2024129672A1 (en) 2022-12-12 2024-06-20 The Broad Institute, Inc. Trafficked rnas for assessment of cell-cell connectivity and neuroanatomy

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021340A1 (en) * 1992-04-22 1993-10-28 Medical Research Council Dna sequencing method
EP0663447A2 (en) * 1993-12-28 1995-07-19 Eiken Chemical Co., Ltd. Method of detecting a specific polynucleotide
WO1999057321A1 (en) * 1998-05-01 1999-11-11 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US6555349B1 (en) * 1993-01-22 2003-04-29 Cornell Research Foundation, Inc. Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
EP1105529B2 (en) 1998-07-30 2013-05-29 Illumina Cambridge Limited Arrayed biomolecules and their use in sequencing
US6245507B1 (en) * 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021340A1 (en) * 1992-04-22 1993-10-28 Medical Research Council Dna sequencing method
EP0663447A2 (en) * 1993-12-28 1995-07-19 Eiken Chemical Co., Ltd. Method of detecting a specific polynucleotide
WO1999057321A1 (en) * 1998-05-01 1999-11-11 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9096898B2 (en) 1998-05-01 2015-08-04 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9133511B2 (en) 2000-10-06 2015-09-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9388463B2 (en) 2001-12-04 2016-07-12 Illumina Cambridge Limited Labelled nucleotides
US9121062B2 (en) 2001-12-04 2015-09-01 Illumina Cambridge Limited Labelled nucleotides
US9410200B2 (en) 2001-12-04 2016-08-09 Illumina Cambridge Limited Labelled nucleotides
US9127314B2 (en) 2002-08-23 2015-09-08 Illumina Cambridge Limited Labelled nucleotides
US9388464B2 (en) 2002-08-23 2016-07-12 Illumina Cambridge Limited Modified nucleotides
US9121060B2 (en) 2002-08-23 2015-09-01 Illumina Cambridge Limited Modified nucleotides
US9410199B2 (en) 2002-08-23 2016-08-09 Illumina Cambridge Limited Labelled nucleotides
US9469873B2 (en) 2006-09-28 2016-10-18 Illumina, Inc. Compositions and methods for nucleotide sequencing
US9528151B2 (en) 2006-12-01 2016-12-27 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US9175342B2 (en) 2007-10-19 2015-11-03 The Trustees Of Columbia University In The City Of New York Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators

Also Published As

Publication number Publication date
AU7537200A (en) 2001-04-30
WO2001023610A2 (en) 2001-04-05
US20030013101A1 (en) 2003-01-16
EP1218543A2 (en) 2002-07-03
US6833246B2 (en) 2004-12-21

Similar Documents

Publication Publication Date Title
WO2001023610A3 (en) Polynucleotide sequencing
DE69417416D1 (en) NEW CATALYST AND METHOD FOR DEHYDRATING DEHYDRABLE HYDROCARBONS
CA2206824A1 (en) Inhibitors of rotamase enzyme activity
HUP9802024A2 (en) Altered thermostable dna polymerases for sequencing
WO1999037806A3 (en) Modified nucleic acid probes and uses thereof
DE68911299D1 (en) ENZYME ELECTRODE AND PRODUCTION METHOD.
DE60314902D1 (en) MISSING PARTNERSHIP AND USE METHOD
CA2163816A1 (en) Electrochemical enzyme biosensor
DE69319771D1 (en) Immobilized enzyme electrode, composition for its production and electrically conductive enzymes
CA2153254A1 (en) Cloning of enterokinase and method of use
PL327994A1 (en) Cyclopylin rotamasic activity inhibitors
DE3585092D1 (en) MELT-PROCESSABLE COPOLYMERS OF TETRAFLUORETHYLENE AND PERFLUOROLEFINES AND METHOD FOR THE PRODUCTION THEREOF.
ATE366822T1 (en) METHOD FOR THE QUANTITATIVE DETECTION OF NUCLEIC ACIDS
EP0654530A3 (en) Ubiquitin carrier enzyme E2-F1, purification, production and use.
WO2001002571A3 (en) An interleukin-1 receptor antagonist and uses thereof
AU8020398A (en) Method for enhancing the activity of an enzyme, bleach composition, detergent composition and process for inhibiting dye transfer
WO1997045554A3 (en) Capture of single stranded nucleic acids
WO2006026577A3 (en) Systems and methods for assigning tasks to derived timers of various resolutions in real-time systems to maximize timer usage
DE69308531D1 (en) Cycloisomaltooligosaccharides; Enzyme and process for its preparation, and process for its production
DE3672920D1 (en) METHOD FOR PRODUCING IMMOBILIZED ENZYMES AND DEVICE FOR IMPLEMENTING THE METHOD.
EP1146124A3 (en) Gustducin materials and methods
DE69728863D1 (en) ENZYME WITH PECTIN ESTERASE ACTIVITY
ATE440964T1 (en) METHOD FOR DETECTING POLYNUCLEOTIDE KINASE AND USE THEREOF AS A MARKER
WO2001060986A3 (en) Esterase enzymes having selective activity
DE69001702D1 (en) CATALYST COMPOSITION, METHOD FOR CRACKING NON-AROMATS AND METHOD FOR ISOMERIZING C8-AROMATIC CARBONATES.

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2000964436

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10113221

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2000964436

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2000964436

Country of ref document: EP