WO2000070346A1 - Dispositif d'analyse quantitative par chromatographie, procede d'analyse quantitative par chromatographie et element de test de chromatographie utilisee - Google Patents
Dispositif d'analyse quantitative par chromatographie, procede d'analyse quantitative par chromatographie et element de test de chromatographie utilisee Download PDFInfo
- Publication number
- WO2000070346A1 WO2000070346A1 PCT/JP2000/003002 JP0003002W WO0070346A1 WO 2000070346 A1 WO2000070346 A1 WO 2000070346A1 JP 0003002 W JP0003002 W JP 0003002W WO 0070346 A1 WO0070346 A1 WO 0070346A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- reaction
- layer
- chromatography
- capture reagent
- analyte
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/86—Investigating moving sheets
Definitions
- the present invention realizes a chromatographic quantitative measurement device, a quantitative measurement method, and a chromatographic test strip that can improve the quantified measurement performance in immunochromatography. Disclosure of the invention
- a mouthmatography-one test piece having a plurality of reaction regions holding a capture reagent capable of being colored by specifically binding to the analyte
- the coloration state due to the specific binding between the capture reagent and the analyte in at least two of the plurality of reaction areas is determined by at least one of optical measurement and image measurement. Measuring, and processing the measurement result to calculate a digitized concentration;
- FIG. 2 shows a manufacturing process of the method for measuring a fixed amount of chromatography according to the embodiment of the present invention.
- the reaction region is formed over the entire region of the reaction layer.
- the shape of the reaction region on which the capture reagent is immobilized is planar, and exists over a wide area on the reaction layer. According to this configuration, since the entire region of the reaction layer is a reaction region, it is possible to measure a color reaction in the entire reaction layer. Furthermore, since the reaction region is uniformly present in the entire reaction layer, more accurate quantitative measurement can be performed even when a color reaction is caused by the permeation state of the liquid sample. The color reaction is performed without being affected by the permeation state of the liquid sample.
- the concentration of the capture reagent immobilized on the reaction layer is constant in all reaction regions. According to this configuration, the color reaction becomes uniform.
- a method for measuring a fixed amount of mouth chromatography which measures the concentration of an analyte contained in a liquid sample according to a typical embodiment of the present invention is as follows.
- the reaction layer carrier 10 includes a support 1, a reaction layer 2, a sample addition layer 3, a labeling reagent holding layer 4, and a water absorbing layer 5.
- the reaction layer carrier 10 is configured such that the sample liquid flows through the sample addition layer 3, the labeling reagent holding layer 4, the reaction layer 2, and the water absorption layer 5 in this order.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Plasma & Fusion (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Description
Claims
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020017000508A KR20010053509A (ko) | 1999-05-13 | 2000-05-11 | 크로마토그래피 정량 측정 장치, 크로마토그래피 정량측정 방법 및 이를 이용한 크로마토그래피 시험편 |
EP00925606A EP1096256A4 (en) | 1999-05-13 | 2000-05-11 | QUANTITATIVE, CHROMATOGRAPHIC MEASURING DEVICE, A CORRESPONDING METHOD, AND A TEST ELEMENT AND APPLICATIONS THEREFOR |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11132432A JP2000321277A (ja) | 1999-05-13 | 1999-05-13 | クロマト定量測定装置 |
JP11/132432 | 1999-05-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000070346A1 true WO2000070346A1 (fr) | 2000-11-23 |
Family
ID=15081246
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2000/003002 WO2000070346A1 (fr) | 1999-05-13 | 2000-05-11 | Dispositif d'analyse quantitative par chromatographie, procede d'analyse quantitative par chromatographie et element de test de chromatographie utilisee |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1096256A4 (ja) |
JP (1) | JP2000321277A (ja) |
KR (1) | KR20010053509A (ja) |
CN (1) | CN100347547C (ja) |
WO (1) | WO2000070346A1 (ja) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1249696B1 (en) * | 2000-09-25 | 2012-09-19 | Panasonic Corporation | Device for chromatographic quantitative measurement |
EP1281966A3 (en) * | 2001-07-30 | 2003-06-18 | Fuji Photo Film Co., Ltd. | Method and apparatus for conducting a receptor-ligand reaction |
EP1333281A3 (en) * | 2002-01-31 | 2004-01-14 | Fuji Photo Film Co., Ltd. | Method and reactor for conducting receptor-ligand association reactions |
KR101254512B1 (ko) * | 2005-01-28 | 2013-04-19 | 모치다 세이야쿠 가부시키가이샤 | 면역 크로마토그래피용 시험구 및 이것을 사용한 반정량방법 |
EP2367002A4 (en) * | 2008-11-20 | 2012-07-11 | Arkray Inc | TEST TOOL AND OPTICAL MEASURING DEVICE |
CN101790685B (zh) * | 2008-11-20 | 2013-10-30 | 爱科来株式会社 | 光学测定装置 |
CN101821624B (zh) | 2008-11-20 | 2014-01-22 | 爱科来株式会社 | 光学测定装置 |
JP5351090B2 (ja) * | 2010-03-31 | 2013-11-27 | 富士フイルム株式会社 | 分析装置および方法 |
US9435806B2 (en) | 2010-09-30 | 2016-09-06 | Sekisui Medical Co., Ltd. | Immunochromatographic test strip and manufacturing method thereof |
CN102384972B (zh) * | 2010-10-19 | 2013-09-11 | 刘江 | 一种基于机器视觉的免疫层析结果判读方法 |
JP5146565B2 (ja) * | 2011-05-13 | 2013-02-20 | パナソニック株式会社 | クロマトグラフィー定量測定方法 |
JP5093379B2 (ja) * | 2011-05-13 | 2012-12-12 | パナソニック株式会社 | クロマトグラフィー定量測定方法 |
JP5093380B2 (ja) * | 2011-05-13 | 2012-12-12 | パナソニック株式会社 | クロマトグラフィー定量測定方法 |
JP5083433B2 (ja) * | 2011-05-30 | 2012-11-28 | パナソニック株式会社 | クロマトグラフィー定量測定装置 |
AT512119B1 (de) * | 2011-10-24 | 2015-10-15 | Scheuringer Kim | Testvorrichtung |
GB2555650B (en) * | 2016-11-08 | 2020-02-12 | Univ Salford | Imaging apparatus and methods |
US11446654B2 (en) | 2017-02-10 | 2022-09-20 | Quidel Corporation | Substrate with channels for controlled fluid flow |
KR102394294B1 (ko) * | 2017-07-05 | 2022-05-04 | 주식회사 나노바이오라이프 | 미세 분할 영역을 가진 진단키트 및 진단키트를 이용한 진단방법 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH055743A (ja) * | 1991-01-31 | 1993-01-14 | Wakunaga Pharmaceut Co Ltd | 測定装置 |
JPH08240591A (ja) * | 1995-01-30 | 1996-09-17 | Bayer Corp | 免疫クロマトグラフィー用試験片上での被検物質の定量法 |
JPH08278305A (ja) * | 1995-04-03 | 1996-10-22 | Nippon Shoji Kk | 免疫学的測定装置 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US581840A (en) * | 1897-05-04 | Tool oe | ||
DE4024544A1 (de) * | 1990-08-02 | 1992-02-06 | Boehringer Mannheim Gmbh | Analyseelement und verfahren zu seiner herstellung |
DE4435728A1 (de) * | 1994-01-19 | 1995-07-20 | Boehringer Mannheim Gmbh | Biotinsilan-Verbindungen und diese Verbindungen enthaltende Bindematrix |
JPH0827830A (ja) * | 1994-07-15 | 1996-01-30 | Sanko Setsubi:Kk | スコップ |
AUPN527995A0 (en) * | 1995-09-07 | 1995-09-28 | Agen Biomedical Limited | Method and apparatus for semiquantification of an analyte |
US6924153B1 (en) * | 1997-03-06 | 2005-08-02 | Quidel Corporation | Quantitative lateral flow assays and devices |
US6183972B1 (en) * | 1998-07-27 | 2001-02-06 | Bayer Corporation | Method for the determination of analyte concentration in a lateral flow sandwich immunoassay exhibiting high-dose hook effect |
-
1999
- 1999-05-13 JP JP11132432A patent/JP2000321277A/ja active Pending
-
2000
- 2000-05-11 WO PCT/JP2000/003002 patent/WO2000070346A1/ja not_active Application Discontinuation
- 2000-05-11 CN CNB00800837XA patent/CN100347547C/zh not_active Expired - Fee Related
- 2000-05-11 KR KR1020017000508A patent/KR20010053509A/ko not_active Application Discontinuation
- 2000-05-11 EP EP00925606A patent/EP1096256A4/en not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH055743A (ja) * | 1991-01-31 | 1993-01-14 | Wakunaga Pharmaceut Co Ltd | 測定装置 |
JPH08240591A (ja) * | 1995-01-30 | 1996-09-17 | Bayer Corp | 免疫クロマトグラフィー用試験片上での被検物質の定量法 |
JPH08278305A (ja) * | 1995-04-03 | 1996-10-22 | Nippon Shoji Kk | 免疫学的測定装置 |
Non-Patent Citations (1)
Title |
---|
See also references of EP1096256A4 * |
Also Published As
Publication number | Publication date |
---|---|
KR20010053509A (ko) | 2001-06-25 |
JP2000321277A (ja) | 2000-11-24 |
CN100347547C (zh) | 2007-11-07 |
EP1096256A1 (en) | 2001-05-02 |
EP1096256A4 (en) | 2001-09-19 |
CN1304490A (zh) | 2001-07-18 |
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