WO2000066709A3 - Zymogen activation system - Google Patents

Zymogen activation system Download PDF

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Publication number
WO2000066709A3
WO2000066709A3 PCT/US2000/009973 US0009973W WO0066709A3 WO 2000066709 A3 WO2000066709 A3 WO 2000066709A3 US 0009973 W US0009973 W US 0009973W WO 0066709 A3 WO0066709 A3 WO 0066709A3
Authority
WO
WIPO (PCT)
Prior art keywords
zymogen
catalytic activity
protease gene
activation
precursor
Prior art date
Application number
PCT/US2000/009973
Other languages
French (fr)
Other versions
WO2000066709A9 (en
WO2000066709A2 (en
Inventor
Andrew Darrow
Jensen Qi
Patricia Andrade-Gordon
Original Assignee
Ortho Mcneil Pharmaceutical Re
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Mcneil Pharmaceutical Re filed Critical Ortho Mcneil Pharmaceutical Re
Priority to EP00922167A priority Critical patent/EP1173590A4/en
Priority to CA002372907A priority patent/CA2372907A1/en
Priority to JP2000615734A priority patent/JP2003531567A/en
Priority to AU42396/00A priority patent/AU4239600A/en
Publication of WO2000066709A2 publication Critical patent/WO2000066709A2/en
Publication of WO2000066709A3 publication Critical patent/WO2000066709A3/en
Publication of WO2000066709A9 publication Critical patent/WO2000066709A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

We describe the DNA sequences encoding an expression vector system that will permit, through limited proteolysis, the activation of expressed zymogen precursor of (S1) serine proteases in a highly controlled and reproducible fashion. The processed expressed protein, once activated, is rendered in a form amenable to measuring the catalytic activity. This catalytic activity of the activated form, is often a more accurate representation of the mature S1 protease gene product relative to the unprocessed zymogen precursor. Thus, this series of zymogen activation constructs represents a significant system for the analysis and characterization of serine protease gene products.
PCT/US2000/009973 1999-04-30 2000-04-13 Zymogen activation system WO2000066709A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00922167A EP1173590A4 (en) 1999-04-30 2000-04-13 Zymogen activation system
CA002372907A CA2372907A1 (en) 1999-04-30 2000-04-13 Zymogen activation system
JP2000615734A JP2003531567A (en) 1999-04-30 2000-04-13 Zymogen activation system
AU42396/00A AU4239600A (en) 1999-04-30 2000-04-13 Zymogen activation system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30316299A 1999-04-30 1999-04-30
US09/303,162 1999-04-30

Publications (3)

Publication Number Publication Date
WO2000066709A2 WO2000066709A2 (en) 2000-11-09
WO2000066709A3 true WO2000066709A3 (en) 2001-02-15
WO2000066709A9 WO2000066709A9 (en) 2002-08-29

Family

ID=23170790

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/009973 WO2000066709A2 (en) 1999-04-30 2000-04-13 Zymogen activation system

Country Status (6)

Country Link
EP (1) EP1173590A4 (en)
JP (1) JP2003531567A (en)
AR (1) AR023818A1 (en)
AU (1) AU4239600A (en)
CA (1) CA2372907A1 (en)
WO (1) WO2000066709A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006271285A (en) * 2005-03-30 2006-10-12 National Institute Of Information & Communication Technology Improved ha tag and screening method using improved ha tag

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C
US5196322A (en) * 1987-12-28 1993-03-23 Eli Lilly And Company Vectors and compounds for expression of zymogen forms of human protein C
US5200340A (en) * 1987-05-22 1993-04-06 Zymogenetics, Inc. Thrombin-activated tissue plasminogen activators
US5665566A (en) * 1993-01-15 1997-09-09 Genetics Institute, Inc. Cloning of enterokinase and method of use
WO1997047737A1 (en) * 1996-06-11 1997-12-18 Boehringer Mannheim Gmbh Recombinant blood-coagulation proteases
WO1998049326A1 (en) * 1997-04-25 1998-11-05 Sembiosys Genetics Inc. Method for cleavage of fusion proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5504001A (en) * 1987-11-25 1996-04-02 Zymogenetics, Inc. Hybrid plasminogen activator
US5342762A (en) * 1991-01-03 1994-08-30 Wisconsin Alumni Research Foundation Fibronectin purification vector
DK82893D0 (en) * 1993-07-08 1993-07-08 Novo Nordisk As PEPTIDE

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200340A (en) * 1987-05-22 1993-04-06 Zymogenetics, Inc. Thrombin-activated tissue plasminogen activators
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C
US5196322A (en) * 1987-12-28 1993-03-23 Eli Lilly And Company Vectors and compounds for expression of zymogen forms of human protein C
US5665566A (en) * 1993-01-15 1997-09-09 Genetics Institute, Inc. Cloning of enterokinase and method of use
WO1997047737A1 (en) * 1996-06-11 1997-12-18 Boehringer Mannheim Gmbh Recombinant blood-coagulation proteases
WO1998049326A1 (en) * 1997-04-25 1998-11-05 Sembiosys Genetics Inc. Method for cleavage of fusion proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KUEN S. ET AL.: "The baculovirus expression vector pBSV-8His directs secretion of histidine-tagged proteins", GENE, vol. 162, no. 2, September 1995 (1995-09-01), pages 225 - 229, XP002934133 *
See also references of EP1173590A4 *

Also Published As

Publication number Publication date
EP1173590A2 (en) 2002-01-23
AU4239600A (en) 2000-11-17
CA2372907A1 (en) 2000-11-09
EP1173590A4 (en) 2003-01-15
JP2003531567A (en) 2003-10-28
AR023818A1 (en) 2002-09-04
WO2000066709A9 (en) 2002-08-29
WO2000066709A2 (en) 2000-11-09

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