WO1994027137A3 - Apparatus and methods for multianalyte homogeneous fluoroimmunoassays - Google Patents

Apparatus and methods for multianalyte homogeneous fluoroimmunoassays Download PDF

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Publication number
WO1994027137A3
WO1994027137A3 PCT/US1994/005567 US9405567W WO9427137A3 WO 1994027137 A3 WO1994027137 A3 WO 1994027137A3 US 9405567 W US9405567 W US 9405567W WO 9427137 A3 WO9427137 A3 WO 9427137A3
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WO
WIPO (PCT)
Prior art keywords
patches
capture molecules
specific
methods
waveguide surface
Prior art date
Application number
PCT/US1994/005567
Other languages
French (fr)
Other versions
WO1994027137A2 (en
Inventor
James N Herron
Douglas A Christensen
Hsu-Kun Wang
Karin D Caldwell
Vera Janatova
Shao-Chie Huang
Original Assignee
Univ Utah Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/064,608 external-priority patent/US5512492A/en
Application filed by Univ Utah Res Found filed Critical Univ Utah Res Found
Priority to CA002162996A priority Critical patent/CA2162996C/en
Priority to JP52581094A priority patent/JP3426602B2/en
Priority to DE69429262T priority patent/DE69429262T2/en
Priority to DK94923161T priority patent/DK0700514T3/en
Priority to AU73116/94A priority patent/AU704947B2/en
Priority to AT94923161T priority patent/ATE209782T1/en
Priority to EP94923161A priority patent/EP0700514B1/en
Priority to US08/263,522 priority patent/US5677196A/en
Publication of WO1994027137A2 publication Critical patent/WO1994027137A2/en
Publication of WO1994027137A3 publication Critical patent/WO1994027137A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Optics & Photonics (AREA)
  • Medicinal Chemistry (AREA)
  • Plasma & Fusion (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide (124) with an integral semi-cylindrical lens (502), and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor (120) and assay method have patches (662) of capture molecules (720) each specific for a different analyte (210), disposed adjacent within a single reservoir (660). The capture molecules (720) are immobilized to the patches (662) on the waveguide surface by site-specific coupling of thiol groups on the capture molecules (720) to photo-affinity crosslinkers (706), which in turn are coupled to the waveguide surface (700) or to a non-specific-binding-resistant coating (702) on the surface (700). The patches (662, 664, 666) of different antibodies are produced by selectively irradiating a portion (714) of the waveguide surface (700) during the process of coupling the photo-affinity crosslinkers (706), the selective irradiation involving a mask (712), a laser light source, or the like.
PCT/US1994/005567 1993-05-18 1994-05-18 Apparatus and methods for multianalyte homogeneous fluoroimmunoassays WO1994027137A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CA002162996A CA2162996C (en) 1993-05-18 1994-05-18 Apparatus and methods for multianalyte homogeneous fluoroimmunoassays
JP52581094A JP3426602B2 (en) 1993-05-18 1994-05-18 Apparatus and method for multi-analyte homogeneous fluorescence immunoassay
DE69429262T DE69429262T2 (en) 1993-05-18 1994-05-18 DEVICE AND METHOD FOR HOMOGENEOUS MULTIANALYT IMMUNO ASSAYS
DK94923161T DK0700514T3 (en) 1993-05-18 1994-05-18 Apparatus and Methods for Homogeneous Multi-Analyte Fluoro Immunoassays
AU73116/94A AU704947B2 (en) 1993-05-18 1994-05-18 Apparatus and methods for multianalyte homogeneous fluoroimmunoassays
AT94923161T ATE209782T1 (en) 1993-05-18 1994-05-18 DEVICE AND METHOD FOR HOMOGENEOUS MULTIANALYTE IMMUNO ASSAY
EP94923161A EP0700514B1 (en) 1993-05-18 1994-05-18 Apparatus and methods for multianalyte homogeneous fluoroimmunoassays
US08/263,522 US5677196A (en) 1993-05-18 1994-06-22 Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/064,608 US5512492A (en) 1993-05-18 1993-05-18 Waveguide immunosensor with coating chemistry providing enhanced sensitivity
US7157993A 1993-06-02 1993-06-02
US064,608 1993-06-02
US071,579 1993-06-02

Publications (2)

Publication Number Publication Date
WO1994027137A2 WO1994027137A2 (en) 1994-11-24
WO1994027137A3 true WO1994027137A3 (en) 1995-01-19

Family

ID=26744692

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/005567 WO1994027137A2 (en) 1993-05-18 1994-05-18 Apparatus and methods for multianalyte homogeneous fluoroimmunoassays

Country Status (9)

Country Link
EP (1) EP0700514B1 (en)
JP (1) JP3426602B2 (en)
AT (1) ATE209782T1 (en)
AU (1) AU704947B2 (en)
CA (1) CA2162996C (en)
DE (1) DE69429262T2 (en)
DK (1) DK0700514T3 (en)
ES (1) ES2169079T3 (en)
WO (1) WO1994027137A2 (en)

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Publication number Publication date
WO1994027137A2 (en) 1994-11-24
DK0700514T3 (en) 2002-03-25
AU7311694A (en) 1994-12-12
ATE209782T1 (en) 2001-12-15
CA2162996C (en) 2008-02-05
CA2162996A1 (en) 1994-11-24
DE69429262T2 (en) 2002-07-25
AU704947B2 (en) 1999-05-06
ES2169079T3 (en) 2002-07-01
JP3426602B2 (en) 2003-07-14
EP0700514B1 (en) 2001-11-28
EP0700514A1 (en) 1996-03-13
JPH08510331A (en) 1996-10-29
DE69429262D1 (en) 2002-01-10

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