US10000757B2 - Gene vector - Google Patents
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- US10000757B2 US10000757B2 US11/921,140 US92114006A US10000757B2 US 10000757 B2 US10000757 B2 US 10000757B2 US 92114006 A US92114006 A US 92114006A US 10000757 B2 US10000757 B2 US 10000757B2
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- C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
- C12N2810/6072—Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses
- C12N2810/6081—Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses rhabdoviridae, e.g. VSV
Definitions
- the present invention relates to gene vectors for use in gene transfer and therapy applications, and to methods of producing them, and uses thereof.
- LVs Lentiviral vectors
- other viral vectors are an attractive tool for gene therapy (Thomas et al., 2003).
- LVs can transduce a broad range of tissues, including nondividing cells such as hepatocytes, neurons and hematopoietic stem cells.
- LVs integrate into target cell genomes and provide long-term transgene expression.
- LVs can provide efficient and stable gene transfer, targeting expression to, or de-targeting expression from, a specific cell type remains difficult. This problem is particularly relevant following in vivo vector administration in which transgene expression may only be desired in a specific cell population, such as tumor cells or hepatocytes, but a broad spectrum of cell types are transduced. De-targeting expression is also important when progenitor or stem cells are transduced, but it is necessary to have transgene expression restricted to only one particular lineage of the differentiated population. To date, most efforts to address this problem have relied on either targeting the vector envelope or engineering tissue-specific promoters. There are, however, limitations with both these methods.
- Tissue-specific promoters which are constructed based on, but not identical to, naturally occuring promoter/enhancer elements, are often weakly expressed in target tissues compared to ubiquitously expressed promoters. In addition, these tissue-specific promoters do not always achieve absolute cell specificity (Follenzi et al., 2002). Transgene expression in non-target cells can occur for a variety of reasons, including ‘leaky’ promoter activity and promoter/enhancer trapping (De Palma et al., 2005). The trapping phenomenon comes about because the vector preferentially integrates at sites of active transcription, which can, in turn, drive transgene transcription independent of the vector's promoter.
- microRNA endogenously expressed microRNA
- WO03/020931 describes an reporter system assay system displaying miRNA provides a method for measuring knockdown of a readily assayed gene. The system is used to determine if siRNAs and chimeric RNAs can decrease expression of the readily assayed luciferase gene.
- US Patent Application 20050266552 describes the construction of a reporter construct suitable for introduction into mammalian cells to create cell lines that can be used for identification of genes involved in miRNA translational repression pathways and/or chemical modulators of such pathways.
- a gene transfer vector suitable for genetic engineering approaches, such as gene therapy, gene transfer and/or regulation of expression of a transgene comprising a miRNA sequence target.
- the miRNA is “operably linked” to the transgene.
- operably linked means that the components described are in a relationship permitting them to function in their intended manner.
- the vector is a viral vector particle comprising a miRNA sequence target.
- the particle comprises the genome (DNA or RNA) of the vector particle, which genome comprises the miRNA sequence target.
- the particle comprises the genome of the vector particle, which RNA genome comprises the miRNA sequence target.
- the particle comprises the RNA genome of the vector particle which RNA genome comprises multiple miRNA sequence targets, which may be in tandem.
- the particle comprises the RNA genome of the vector particle which RNA genome comprises multiple different miRNA sequence target, which may be in tandem.
- More then one copy of a miRNA target sequence included in the vector may increase the effectiveness of the system.
- different miRNA target sequences could be included.
- vectors which express more than one transgene may have the transgene under control of more than one miRNA target sequence, which may or may not be different.
- the miRNA target sequences may be in tandem, but other arrangements are envisaged, such the use of antisense orientations. Antisense orientations may be useful in the production of viral particles to avoid expression of gene products which may otherwise be toxic to the producer cells.
- the particle comprises the genome of the vector particle, which RNA genome comprises a transgene.
- the particle is derivable from a lentivirus.
- the gene transfer vector is in the form of a non-viral gene transfer vector.
- the gene transfer vector may comprise, or be in the form of, an expression vector or plasmid which comprises the miRNA target sequence and optionally a transgene.
- Expression vectors as described herein comprise regions of nucleic acid containing sequences capable of being transcribed. Thus, sequences encoding mRNA, tRNA and rRNA are included within this definition.
- the gene vector or gene transfer vector of the present invention may be used to deliver a transgene to a site or cell of interest.
- the vector of the present invention may be delivered to a target site by a viral or non-viral vector.
- a vector is a tool that allows or facilitates the transfer of an entity from one environment to another.
- some vectors used in recombinant DNA techniques allow entities, such as a segment of DNA (such as a heterologous DNA segment, such as a heterologous cDNA segment), to be transferred into a target cell.
- the vector may then serve to maintain the heterologous DNA within the cell or may act as a unit of DNA replication.
- examples of vectors used in recombinant DNA techniques include plasmids, chromosomes, artificial chromosomes or viruses.
- Non-viral delivery systems include but are not limited to DNA transfection methods.
- transfection includes a process using a non-viral vector to deliver a gene to a target mammalian cell.
- Typical transfection methods include electroporation, DNA biolistics, lipid-mediated transfection, compacted DNA-mediated transfection, liposomes, immunoliposomes, lipofectin, cationic agent-mediated, cationic facial amphiphiles (CFAs) (Nature Biotechnology 1996 14; 556), and combinations thereof.
- CFAs cationic facial amphiphiles
- Viral delivery systems include but are not limited to adenovirus vector, an adeno-associated viral (AAV) vector, a herpes viral vector, retroviral vector, lentiviral vector, baculoviral vector.
- Other examples of vectors include ex vivo delivery systems, which include but are not limited to DNA transfection methods such as electroporation, DNA biolistics, lipid-mediated transfection, compacted DNA-mediated transfection.
- vector particle refers to the packaged retroviral vector, that is preferably capable of binding to and entering target cells.
- the components of the particle may be modified with respect to the wild type retrovirus.
- the Env proteins in the proteinaceous coat of the particle may be genetically modified in order to alter their targeting specificity or achieve some other desired function.
- the viral vector preferentially transduces a certain cell type or cell types.
- the viral vector is a targeted vector, that is it has a tissue tropism which is altered compared to the native virus, so that the vector is targeted to particular cells.
- the particle comprising the miRNA target sequence is one targeted by mir-142as (also called hsa-mir-142-3p), let-7a, mir-15a, mir-16, mir-17-5p, mir-19, mir-142-5p, mir-145, mir-218 miRNA.
- mir-142as also called hsa-mir-142-3p
- let-7a mir-15a
- mir-16 mir-17-5p
- mir-19 mir-142-5p
- mir-145 mir-218 miRNA.
- a set of DNA constructs for producing the viral vector particle comprising a DNA construct encoding a packagable vector genome comprising a miRNA sequence target, and optionally a transgene.
- packagable vector genome we mean that the vector genome is in an environment where it can be packaged into a viral vector particle. This generally requires the present of Gag-Pol and Env.
- a process for preparing a viral vector particle comprising introducing the set of DNA constructs of claim into a host cell, and obtaining the viral vector particle.
- a viral vector particle produced by the process of the present invention.
- composition comprising the gene vector or vector particle according to the present invention together with a pharmaceutically acceptable diluent, excipient or carrier.
- a cell infected or transduced with the vector particle of the present invention comprises the corresponding miRNA.
- the cell may be transduced or infected in an in vivo or in vitro scenario.
- the cell may be derived from or form part of an animal, preferably a mammal, such as a human or mouse.
- a mammal such as a human or mouse.
- the present invention is useful in providing transgenic animals e.g., for use as disease models.
- the mammal is a non-human mammal.
- transgene dysregulation When applied to integrating vectors, it can circumvent problems of transgene dysregulation, which can occur as a result of insertional position effects (integration next to strong promoter/enhancer sequences that override the transcriptional control of the vector-internal promoter) and enable highly cell-specific patterns of transgene expression.
- Vectors such as viral including lentiviral vectors, for transgene expression for gene transfer and therapy can be engineered with miRNAs target sequence in order to be recognized by endogenous miRNAs cell type specific, thus regulating transgene expression in a subset of cells.
- miRNA target sequences can be used to obtain vectors with highly specific cell expression patterns.
- the inventors demonstrate this with 9 different miRNAs, including let-7a, mir-15a, mir-16, mir-17-5p, mir-19, mir-142-3p, mir-142-5p, mir-145, and mir-218. They show that the concentration of a miRNA within a cell can be used to predict the expression profile of a vector.
- the method described by this patent provides a simple method for designing vectors with highly specific cell expression patterns.
- transgene expression from a ubiquitously expressed promoter can be prevented precisely in a hematopoietic cell line by using a vector that displays the mir-142-3p target sequence in the transgene's 3′UTR, as shown in the figure below, because miR-142-3p has a cell-type specific expression pattern in hematopoietic tissues.
- this system does not reduce transgene expression in other cell types.
- the inventors demonstrate that incorporating a target sequence for mir-19a into the vector, transgene expression can be suppressed in 293T producer cells, which express mir-19a to high levels, and that this does not negatively effect the production of the vector.
- This strategy provides an important, and hitherto unavailable, means of producing high titer vectors which carry a toxic transgene.
- a further usage of our invention is in the design of a vector system that expresses two transgenes with distinct expression profiles.
- the inventors demonstrate this by incorporating a target sequence for mir-142-3p into one of the two genes of a bidirectional lentiviral vector.
- kidney cells both transgenes are expressed because mir-142-3p is not present.
- hematopoietic cells only one of the two transgenes is expressed.
- This construct provides proof-of-principle that miRNA regulation can be used to divergently regulated two transgenes from a single vector construct.
- embryonic stem cells may be transduced by a single vector where gene 1 is a toxic transgene and gene 2 is a transgene that provides growth advantage to the cells.
- Gene 1 would contain a miRNA target sequence specific for neurons and gene 2 would contain a miRNA target sequence specific for embryonic stem cells. In this way, transduced embryonic stem cells can be directed to differentiated into neurons, and any cells which do not differentiate, and remain as undifferentiated embryonic stem cells would be selectively killed.
- the inventors show that transfer of a miRNA target sequence into a cell, even at high copy, does not perturb the natural activity or expression of the endogenous miRNA, which is targeting the vector sequence.
- the miRNA-mediated approach for restricting gene expression has several advantages over other strategies of regulating transgenes.
- most efforts to limit expression from professional antigen presenting cells (APCs) rely on tissue-specific promoters (Brown et al., 2004b; Follenzi et al., 2004; Mingozzi et al., 2003).
- tissue-specific promoters Brown et al., 2004b; Follenzi et al., 2004; Mingozzi et al., 2003.
- This approach can successfully limit expression to target cells, ‘leaky’ expression in a fraction of non-target cells is observed. This occurs because the reconstituted promoter, modified for inclusion into a vector system, often loses some of its cell specificity and also because vector integration near active promoters and enhancers can activate the tissue-specific promoter and drive transgene expression.
- miRNA-mediated silencing occurs at the post-transcriptional level, promoter and enhancer trapping is irrelevant.
- miRNA-regulation can be used to effectively de-target transgene expression from a particular cell type, while still allowing for broad tissue expression, as we have described here.
- miRNA regulation may also be used as a complimentary approach to regulating a transgene by promoter/enhancers. By including the miRNA target sequence in expression cassettes already under the control of a tissue-specific promoter, we add an additional layer of regulation which will eliminate off-target expression.
- transgene expression in non-target cells which is caused by events occurring at the level of transcriptional regulation, may be overcome by utilizing the miRNA system of gene regulation that acts post-transcriptionally. Restricting transgene expression to a particular cell type may also decrease the potential efficacy of gene transfer by limiting the pool of cells expressing the transgene.
- miRNA regulation which de-targets rather than targets gene expression and functions at the post-transcriptional level, may provide a unique means for overcoming the limitations of current gene delivery systems.
- miRNA regulation By preventing transgene expression in hematopoietic lineages, while permitting high levels of expression in non-hematopoietic cells, we reasoned that miRNA regulation could enable strong and stable gene transfer in the absence of an immune response.
- mice treated with LV.PGK.GFP.142-3pT had a significant frequency of GFP positive hepatocytes at 2 weeks following vector administration.
- GFP expression was found to persist for over 120 days post-injection (the last time point analyzed).
- the miRNA-regulation strategy was also effective for preventing an immune response to a circulating antigen.
- hFIX human Factor IX
- the present invention Upon vector administration in vivo, the present invention will avoid vector expression in antigen presenting cells of the immune system, which are part of the hematopoietic system, and thereby prevent the initiation of an immune response against the transgene.
- the present invention when applied to a tissue-specific promoter which targets expression to hepatocytes, it would allow suppressing ectopic expression in a transduced APC. This would potentially solve a major hurdle and long-standing problem in gene transfer; namely, immune-mediated rejection of the transferred gene.
- FIG. 1 a Schematic representation of a miRNA-regulated lentiviral vector system. Shown here is the parent lentiviral vector encoding enhanced green fluorescent protein (eGFP) under the transcriptional control of the ubiquitously expressed human PGK promoter (LV.PGK.GFP), and a modified vector, which contains 4 tandem copies of a sequence targeted by an endogenous miRNA (LV.PGK.GFP.mirT)
- eGFP enhanced green fluorescent protein
- LV.PGK.GFP ubiquitously expressed human PGK promoter
- LV.PGK.GFP.mirT modified vector, which contains 4 tandem copies of a sequence targeted by an endogenous miRNA
- FIG. 1 b Schematic representation of a divergently regulated lentiviral vector system utilizing miRNA regulation. Shown here is the parent bidirectional lentiviral vector encoding eGFP and the mutated low-affinity nerve growth factor receptor ( ⁇ LNGFR) under the transcriptional control of a bidirectional promoter construct (Bd.LV), which enables co-ordinate transcription of two transgenes as distinct transcripts. Bd.LVs were modified to include mirT sequences in the 3′ untranslated region (3′UTR) of the eGFP expression cassette.
- 3′UTR 3′ untranslated region
- FIG. 1 c Schematic representation of a hepatocyte-specific, miRNA-regulated lentiviral vector system. Shown here is the parent lentiviral vector encoding human clotting factor IX (hFIX) under the transcriptional control of a synthetic liver-specific promoter/enhancer element. (LV.ET.hFIX), and a modified vector, which contains 4 tandem copies of a sequence targeted by an endogenous miRNA (LV.ET.hFIX.mirT)
- FIG. 2 a miRNA profiling analysis. Expression analysis of selected miRNAs in 293T and U937 cells by real-time PCR. Expression levels are reported relative to let-7a, a constitutively expressed, ‘housekeeping’ miRNA.
- FIG. 2 b miRNA regulation can be used to de-target expression from hematopoietic lineages. FACS analysis of 293T (kidney origin), U937 (monocyte origin) and primary dendritic cells (peripheral blood-derived) transduced with dose-matched concentrations of the indicated LV at 14 days post-transduction. An LV containing the liver-specific Albumin promoter (LV.ALB3.GFP), is shown for comparison of off-target activity of this promoter. The histograms are representative of three independent experiments. Vector copies per genome (C/G) were determined by Taqman analysis. Shown in grey are the untransduced cells.
- C/G Vector copies per genome
- FIG. 2 c miRNA regulation can be exploited to construct a vector for divergent regulation of two transgenes. FACS analysis of GFP and ⁇ LNGFR expression from 293T and U937 cells transduced with closely matched concentrations of Bd.LV expressing GFP, with or without the mir-142-3pT, and ⁇ LNGFR, 14 days post-transduction. Dotplots are representative of two independent experiments.
- FIG. 2 d The miRNA-regulated vector design can be used to construct a variety of vectors which are regulated by different endogenous miRNA, and mediate diverse vector expression profiles. FACS analysis of GFP and ⁇ LNGFR expression from 293T and U937 cells transduced with closely matched concentrations of Bd.LV expressing GFP, with or without the indicated mirT sequences, and ⁇ LNGFR, at 14 days post-transduction.
- FIG. 3 a Quantitative RT-PCR analysis of GFP expression from 293T and U937 cells transduced by LV.PGK.GFP or LV.PGK.GFP.142-3pT.
- cDNA is from cells presented in FIG. 1 b . All samples were normalized to GAPDH expression and values are reported relative to transcripts detected from 293T cells transduced with 10 5 TU/mL LV.PGK.GFP, which was set as the calibrator.
- FIG. 3 b Quantitative RT-PCR analysis of GFP and ⁇ LNGFR expression from U937 cells transduced by the indicated Bd.LV.
- the cDNA was taken from the cells presented in FIG. 1 c . All values are reported relative to the level of ⁇ LNGFR transcripts detected in cells transduced with 10 5 TU/mL Bd.LV.
- FIG. 3 c Northern blot analysis of cells transduced by LV and BDd.LV with or without mir-142-3pT (shown in FIGS. 1 b and 1 c , respectively). Twenty micrograms of total RNA was loaded for each sample and probed for GFP. The expected size of the GFP transcript is indicated by arrows for the LV (top) and Bd.LV (bottom).
- FIG. 3 d U937 cells repeatedly infected with LV.PGK.GFP.142-3pT to obtain increasing vector content. GFP was measured by FACS analysis. Average vector C/G for the cell population are indicated. A regression analysis showing the relationship between increasing vector dose and transgene expression for LV.PGK.GFP.142-3pT is included (right). Note that in U937 cells a single copy of LV.PGK.GFP (bottom left panel) expresses GFP to higher levels than 175 C/G of LV.PGK.GFP.142-3pT.
- FIG. 3 e The robustness of mir-142-3p-mediated RNA interference was measured by superinfection of U937 cells containing 4 C/G of LV.PGK.GFP.mir-142-3pT with increasing concentrations of LV.PGK. ⁇ LNGFR.mir-142-3pT. Taqman analysis was used to detect the vector copy number of superinfected cells, and changes in GFP and ⁇ LNGFR expression were measured by FACS analysis.
- FIG. 4 miRNA regulation can be exploited to prevent transgene expression in producer cells without reducing vector titer.
- Transgene expression and production titer of three different lentiviral vector constructs were compared. Histograms show the GFP expression in 293T cells during vector production. Dotplots present the GFP expression in 293T cells following transduction with the produced vectors. Constructs pLV.PGKas.GFPas.CTEas.polyAas and pLV.PGKas.GFPas.19aT.CTEas.polyAas have the expression cassettes in antisense orientation.
- FIG. 5 a miRNA-regulated vectors can be designed to achieve selective de-targeting of expression from a particular cellular lineage in vivo. Confocal microscopy analysis of liver of nude mice injected by tail vein 2 weeks prior with the indicated LV. Images are representative of 3 mice. GFP was visualized by direct fluorescence. Liver sections were immunostained for (left) the macrophage-specific marker F4/80 and (right) for the endothelial cell marker CD31. Virtually none of the F4/80+ Kupffer cells expressed GFP to detectable levels when the mir-142-3pT vector was used, whereas many of these cells expressed GFP when transduced by the other vectors. Note that the CD31+ liver sinusoidal endothelial cells expressed GFP upon transduction by all vectors, including LV.PGK.GFP.142-3pT (arrows).
- FIG. 5 b miRNA-regulated vectors can be designed to achieve selective de-targeting of expression from a particular cellular lineage in vivo. Spleen sections from the same mice as above were immunostained for the pan-leukocyte CD45 marker. LV.PGK.GFP.142-5pT effectively de-targeted GFP expression from the CD45+ leukocytes, but permitted strong GFP expression in the non-hematopoietic stromal cells (CD45-negative) of the marginal zone sinus.
- FIG. 5 c miRNA-regulated lentiviral vectors can be designed to prevent transgene expression in hematopoietic cells following intravenous vector injection. FACS analysis of GFP expression from splenocytes of LV.PGK.GFP- and LV.PGK.GFP.142-3pT-treated animals.
- FIG. 6 a miRNA-regulated vectors can be designed to prevent transgene expression in hematopoietic lineage cells in vivo, even at high vector copy. FACS analysis of GFP expression in the peripheral blood and bone marrow from representative TgN.PGK.GFP.142-3pT (24 C/G) and TgN.PGK.GFP (4 C/G) transgenic mice showing virtually undetectable transgene expression despite the high number of vector copies carried by these mice.
- FIG. 6 b miRNA-regulated vectors can be designed to segregate gene expression between hematopoietic and non-hematopoietic lineages of transgenic mice. Immunofluorescence of the indicated organs from the above mice. GFP was visualized by direct fluorescence. Hematopoietic lineage cells were marked by CD45 immunostaining in all organs analyzed except for the thymus, where CD3 was used to mark thymocytes. In TgN.PGK.GFP mice, pan-cellular GFP expression was detected in the parenchyma and stroma of all organs. Hematopoietic lineage cells appear yellow because of overlap between CD45 staining and GFP expression.
- GFP expression in PGK.GFP.142-3pT transgenic mice was selectively suppressed in the CD45+ Kupffer cells (liver), alveolar (lung) and lamina propria (gut) macrophages, which appear red and are indicated by arrows.
- CD45+ Kupffer cells liver
- alveolar lung
- lamina propria gut
- GFP expression was also negative in all hematopoietic lineage cells, despite strong expression within the stroma of these organs.
- FIG. 7 a miRNA-regulated LV enables stable gene transfer in immunocompetent mice. Confocal immunofluorescence analysis of liver and spleen sections from Balb/c mice administered the indicated LV. GFP was visualized in the liver by direct fluorescence; Kupffer cells, CD8+ T-cells, or endothelial cells were detected by staining with anti-F4/80, anti-CD8, or anti-CD31, respectively. The GFP+ cells of LV.PGK.GFP and LV.ALB.GFP mice were cleared from the liver by 2 weeks, which correlated with the presence of CD8+ T-cell infiltrates. In contrast, abundant GFP+ hepatocytes and endothelial cells persisted for >120 days (longest time point analyzed) in mice injected with LV.PGK.GFP.142-3pT.
- FIG. 7 b GFP+ cells in the liver of day 70 LV.PGK.GFP.142-3pT-treated mice had the typical morphology of hepatocytes or were CD31+ endothelial cells (arrows). This demonstrates a novel aspect of this approach, which is selective de-targeting of expression from a particular cell type, while permitting transgene expression in a broad range of cell lineages.
- FIG. 7 c Hematoxylin and eosin (H&E) staining showing normal histology and absence of mononuclear cell infiltration in LV.PGK.GFP.142-3pT mice at 42 days post-injection
- FIG. 7 d Analysis of the spleen of immunocompetent mice injected 5 days prior with the indicated vector.
- GFP expression from the mir-142-3pT vector was mainly observed at the marginal zone sinus (MS); some of these GFP+ cells expressed ⁇ -smooth muscle actin ( ⁇ -SMA) and were identified as fibroblast-like stromal cells (arrows).
- ⁇ -SMA ⁇ -smooth muscle actin
- arrows scattered GFP+ cells, including some CD45+ hematopoietic cells, were present in the spleen of LV.ALB.GFP mice (arrow). This further demonstrates that the miRNA-regulation strategy can provide an improved means of transgene regulation over tissue-specific promoters.
- FIG. 8 a miRNA-regulated lentiviral vectors mediate stable correction of hemophilia B in a mouse model.
- Hemophilia B mice Factor IX knock-out mice were injected via tail with a lentiviral vector encoding hFIX under the control of the hepatocyte-specific ET promoter (LV.ET.hFIX) or a modified LV.ET.hFIX containing the mir-142-3pT sequence in the 3′UTR of the transgene (LV.ET.hFIX.142-3pT).
- hFIX antigen was determined by a hFIX-specific ELISA (top), while FIX clotting activity was determined by measurement of the activated partial thromboplastin time (bottom). Results are presented as the mean plus or minus the standard error from three mice treated per vector.
- FIG. 9A shows the mature hsa-mir-142 stem loop sequence (SEQ ID NO: 20).
- FIG. 9B shows the sequence of the mir-142 as target (SEQ ID NO: 21).
- MIRNAS MIRNAS
- miRNAs are small, RNA molecules encoded in the genomes of plants and animals. These highly conserved, ⁇ 21-mer RNAs regulate the expression of genes by binding to specific mRNAs (He and Hannon, 2004).
- miRNAs are a family of small, non-coding RNAs that regulate gene expression in a sequence-specific manner.
- miRNAs have been cloned and sequenced from mouse, human, Drosphila, C, elegans and Arabidopsis . Examples of such sequences may be found on www.sanger.ac.uk (Griffiths-Jones et al., 2006). Further miRNA target sequences may be searched at www.miRNA.org.
- miRNA expression profiles appear to vary from tissue to tissue but a similar for identical tissues in different individuals (Baskerville and Bartel, 2005). Determining an miRNA with the desired expression profile may be achieved using techniques known to those skilled in the art. Once, the miRNA has been identified the corresponding target sequence can readily be determined using, for example, the databases indicated above.
- mirVanaTM miTNA Probe Set and mirVanaTM miTNA Labelling Kit available from Ambion, Inc. may be used to compare the miRNA expression profiles in human tissues according to the manufacturer's instructions.
- tissue-specific miRNAs Another common way of identifying tissue-specific miRNAs is using Northern Blot.
- An example of such a technique is described in Lagos-Quintana M et al, Current Biol (2002) 12:735-739 in which they identify 34 novel miRNAs by tissue-specific cloning of approximately 21-nucleotide RNAs from mouse (Lagos-Quintana et al., 2002).
- mice brain-, liver- and heart-specific miRNAs were also detected in the human counterpart organs (miRNA expression was not examined in human kidney, lung or spleen), with the exception of miR-183 in the human brain.
- the levels of 14 of these were detected in a particular mouse organ at levels at least two-fold higher than in any other organ; these included: seven brain-enriched miRNAs (miR-9*, -125a, -125b, -128, -132, -137, -139), three skeletal muscle-enriched miRNAs (miR-1d, -133, -206), two kidney-enriched miRNAs (miR-30b, -30c), and one spleen-enriched miRNA (miR-99a).
- Baskerville & Bartel, RNA (2005) 11:241-247 discloses a microarray profiling survey and the expression patterns of 175 human miRNAs across 24 different human organs. The results show that proximal pairs of miRNAs are generally coexpressed (Baskerville and Bartel, 2005). In addition, an abrupt transition in the correlation between pairs of expressed miRNAs occurs at a distance of 50 kb, implying that miRNAs separated by ⁇ 50 kb typically derive from a common transcript. Some miRNAs are within the introns of host genes. Intronic miRNAs are usually coordinately expressed with their host gene mRNA, implying that they also generally derive from a common transcript, and that in situ analyses of host gene expression can be used to probe the spatial and temporal localization of intronic miRNAs.
- RNA-specific oligonucleotide microarray system that enables efficient analysis of the expression of the human miRNAs identified so far. It shows that the 60-mer oligonucleotide probes on the microarrays hybridize with labeled cRNA of miRNAs, but not with their precursor hairpin RNAs, derived from amplified, size-fractionated, total RNA of human origin. Signal intensity is related to the location of the miRNA sequences within the 60-mer probes, with location at the 5′ region giving the highest signals, and at the 3′ end, giving the lowest signals.
- Mansfield J et al, Nature Genetics (2004) 36:1079-1083 discloses the tissue-specific expression of several miRNAs during embryogenesis, including miR-10a and miR-196a (Mansfield et al., 2004).
- miR-181 a miRNA specifically expressed in B cells within mouse bone marrow (Chen and Lodish, 2005). It also discloses that some human miRNAs are linked to leukemias; the miR-15a/miR-16 locus is frequently deleted or down-regulated in patients with B cell chronic lymphocytic leukemia and miR-142 is at a translocation site found in a case of aggressive B cell leukemia. It is stated that these results indicate that miRNAs may be important regulators of mammalian hematopoiesis.
- miRNA References Tissue-specific expression patterns of mammalian miRNAs ES-cell specific miR-296 a Expressed in ES cells, but miR-21 and miR-22 a upregulated on differentiation Expressed in both ES cells miR-15a, miR-16, miR-19b, miR-92, miR-93 a and various adult tissues miR-96, miR-130 and miR-130b Enriched during mouse miR-128, miR-19b, miR-9, miR-125b, miR-131 b, c brain development miR-178, miR-124a, miR-266 and miR-103 Enriched in adult brain miR-9*, miR-125a, miR-125b, miR-128, miR-132 b miR-137, miR-139, miR-7, miR-9, miR124a, miR-124b, miR-135, miR-153, miR-149, miR-183, miRNA-9*, miR-125a, miR-124
- a vector could be created which includes target sequences of miR-21 and miR-22, two miRNAs upregulated following embryonic stem cell (ESCs) differentiation (Houbaviy et al., 2003), tethered to a suicide gene such as thymidine kinase.
- This vector could serve to selectively kill undifferentiated ESCs in ESC-derived tissue, a much desired safety control for bringing ESC-based therapies to the clinic.
- miRNA-regulated vector design Another possible use of the miRNA-regulated vector design would be in the treatment of cancer.
- miR-15 and mir-45 are downregulated in chronic lymphocytic leukaemias and breast cancer (Calin et al., 2004a; Calin et al., 2004b; Iorio et al., 2005).
- the miR-15 or mir-145 target sequence could be included in a vector expressing a toxic transgene. Normal cells expressing miR-15 or mir-145, including vector producing cells, would suppress production of the toxin and thus survive, whereas transduced tumor cells, no longer expressing miR-15 or mir-145, would readily produce the toxin gene and die.
- miRNA-regulated vector design Another possible use of the miRNA-regulated vector design would be to prevent vector mobilization from transduced hematopoietic cells which become superinfected with wild-type virus.
- the miRNA target sequence could also be included in a region of the vector distinct from the expression cassette for the transgene.
- the miRNA vector may be used in conjunction with a bidirectional promoter (Amendola et al., 2005). These vectors, which have the unique property that they produce two distinct mRNA transcripts from a single promoter, can be modified to include miRNA target sequences in one or both of the expression cassettes.
- mir-142-3pT to transgene 1, but not transgene 2 would enable ubiquitous expression of transgene 2, while preventing expression of transgene 1 in hematopoeitic cells. This design will enable divergent regulation of two transgenes, a feat not possible with current technologies.
- the miRNA may be used with a suitable gene vector, i.e. a vector suitable for delivering a gene (transgene) of interest, such as a viral vector. Examples of these are described below.
- Retroviridae is a family of retroviruses that differs in nucleotide and amino acid sequence, genome structure, pathogenicity, and host range. This diversity provides opportunities to use viruses with different biological characteristics to develop different therapeutic applications.
- the efficiency, the ability to target certain tissue or cell type, the expression of the gene of interest, and the safety of retroviral-based systems are important for successful application of gene therapy.
- Significant efforts have been dedicated to these areas of research in recent years.
- Various modifications have been made to retroviral-based vectors and helper cells to alter gene expression, target delivery, improve viral titers, and increase safety.
- the present invention represents an improvement in this design process in that it acts to efficiently deliver genes of interest into such viral vectors.
- Viruses are logical tools for gene delivery. They replicate inside cells and therefore have evolved mechanisms to enter the cells and use the cellular machinery to express their genes. The concept of virus-based gene delivery is to engineer the virus so that it can express the gene of interest. Depending on the specific application and the type of virus, most viral vectors contain mutations that hamper their ability to replicate freely as wild-type viruses in the host.
- viruses from several different families have been modified to generate viral vectors for gene delivery. These viruses include retroviruses, lentivirus, adenoviruses, adeno-associated viruses, herpes simplex viruses, picomaviruses, and alphaviruses.
- the present invention preferably employs retroviruses, including lentiviruses.
- retroviral vector for gene delivery must be efficient, cell-specific, regulated, and safe. The efficiency of delivery is important because it can determine the efficacy of the therapy. Current efforts are aimed at achieving cell-type-specific infection and gene expression with retroviral vectors. In addition, retroviral vectors are being developed to regulate the expression of the gene of interest, since the therapy may require long-lasting or regulated expression. Safety is a major issue for viral gene delivery because most viruses are either pathogens or have a pathogenic potential. It is important that during gene delivery, the patient does not also inadvertently receive a pathogenic virus that has full replication potential.
- Retroviruses are RNA viruses that replicate through an integrated DNA intermediate. Retroviral particles encapsidate two copies of the full-length viral RNA, each copy containing the complete genetic information needed for virus replication. Retroviruses possess a lipid envelope and use interactions between the virally encoded envelope protein that is embedded in the membrane and a cellular receptor to enter the host cells. Using the virally encoded enzyme reverse transcriptase, which is present in the virion, viral RNA is reverse transcribed into a DNA copy. This DNA copy is integrated into the host genome by integrase, another virally encoded enzyme. The integrated viral DNA is referred to as a provirus and becomes a permanent part of the host genome. The cellular transcriptional and translational machinery carries out expression of the viral genes.
- the host RNA polymerase II transcribes the provirus to generate RNA, and other cellular processes modify and transport the RNA out of the nucleus.
- a fraction of viral RNAs are spliced to allow expression of some genes whereas other viral RNAs remain full-length.
- the host translational machinery synthesizes and modifies the viral proteins.
- the newly synthesized viral proteins and the newly synthesized full-length viral RNAs are assembled together to form new viruses that bud out of the host cells.
- retroviruses can be classified into simple and complex retroviruses.
- Simple and complex retroviruses encode gag (group-specific antigen), pro (protease), pol (polymerase), and env (envelope) genes.
- gag group-specific antigen
- pro pro
- pol polymerase
- env envelopee
- complex retroviruses also encode several accessory genes.
- Retroviruses can also be classified into oncoviruses, lentiviruses, and spumaviruses. Most oncoviruses are simple retroviruses. Lentiviruses, spumaviruses, and some oncoviruses are complex retroviruses. Currently, all three types of viruses are being exploited as gene therapy tools. Examples of each type will be discussed below.
- Murine leukemia virus is example of an oncovirus
- human immunodeficiency virus 1 HIV-1
- human foamy virus is an example of a spumavirus
- a replication-competent retrovirus When a replication-competent retrovirus infects a natural host cell, it can form a provirus in the host genome, express viral genes, and release new infectious particles to infect other hosts. In most gene therapy applications, it is not desirable to deliver a replication-competent virus into a patient because the virus may spread beyond the targeted tissue and cause adverse pathogenic effects. Therefore, in most retroviral systems designed for gene delivery, the viral components are divided into a vector and a helper construct to limit the ability of the virus to replicate freely.
- vector generally refers to a modified virus that contains the gene(s) of interest (or transgene) and cis-acting elements needed for gene expression and replication. Most vectors contain a deletion(s) of some or all of the viral protein coding sequences so that they are not replication-competent.
- Helper constructs are designed to express viral genes lacking in the vectors and to support replication of the vectors. The helper function is most often provided in a helper cell format although it can also be provided as a helper virus or as cotransfected plasmids.
- Helper cells are engineered culture cells expressing viral proteins needed to propagate retroviral vectors; this is generally achieved by transfecting plasmids expressing viral proteins into culture cells. Most helper cell lines are derived from cell clones to ensure uniformity in supporting retroviral vector replication. Helper viruses are not used often because of the likelihood that a replication-competent virus could be generated through high frequency recombination. Helper functions can also be provided by transient transfection of helper constructs to achieve rapid propagation of the retroviral vectors.
- retroviral vectors are maintained as bacterial plasmids to facilitate the manipulation and propagation of the vector DNA.
- These double-stranded DNA vectors can be introduced into helper cells by conventional methods such as DNA transfection, lipofection, or electroporation.
- the helper cell shown expresses all of the viral proteins (Gag, Gag-Pol, and Env) but lacks RNA containing the packaging signal. Viral RNA is necessary for the formation and release of infectious viral particles, but it is not necessary for the formation of “empty” noninfectious viral particles.
- vector DNA is introduced into the helper cells, vector RNA containing a packaging signal is transcribed and efficiently packaged into viral particles.
- the viral particles contain viral proteins expressed from helper constructs and RNA transcribed from the vector.
- viral particles can infect target cells, reverse transcribe the vector RNA to form a double-stranded DNA copy, and integrate the DNA copy into the host genome to form a provirus.
- This provirus encodes the gene(s) of interest and is expressed by the host cell machinery.
- the vector does not express any viral proteins, it cannot generate infectious viral particles that can spread to other target cells.
- Helper cells are designed to support the propagation of retroviral vectors.
- the viral proteins in the helper cells are expressed from helper constructs that are transfected into mammalian cells. Helper constructs vary in their mode of expression and in the genes they encode.
- helper cell lines that were initially developed, all of the viral genes were expressed from one helper construct. Examples of these helper cells are C3A2 and -2.
- the helper constructs for these cell lines were cloned proviral DNAs that lacked the packaging signals.
- helper cells can support efficient propagation of retroviral vectors.
- replication-competent viruses can be frequently generated during the propagation of the viral vector.
- the helper construct contains most of the viral genome and thus shares significant sequence homology with the retroviral vector. The sequence homology can facilitate recombination between the helper construct and the retroviral vector to generate replication-competent viruses.
- helper RNA lacks the packaging signal, it can still be packaged into a virion with a low efficiency (approximately 100- to 1,000-fold less than RNAs containing). Retroviral recombination occurs frequently between the two copackaged viral RNAs to generate a DNA copy that contains genetic information from both parents. If the helper RNA and the vector RNA are packaged into the same virion, the large regions of sequence homology between the two RNAs can facilitate homologous recombination during reverse transcription to generate a replication-competent virus. A similar recombination event can also occur between the helper RNA and RNA derived from an endogenous virus at a lower efficiency to generate replication-competent viruses.
- helper cell lines using “split genomes”, including CRIP, GP+envAm12, and DSN.
- the viral Gag/Gag-Pol polyproteins are expressed from one plasmid and the Env proteins are expressed from another plasmid.
- the two helper constructs also contain deletions of viral cis-acting elements to reduce or eliminate sequence homology with the retroviral vector.
- genes encoding viral proteins are separated into two different constructs and the viral cis-acting elements are located in the vector. Therefore, several recombination events have to occur to reconstitute the viral genome. In addition, reducing the regions of homology decreases the probability that these recombination events will occur. Therefore, helper cells containing split-genome helper constructs are considered safer than helper cells containing one-genome helper constructs.
- helper cell lines In contrast to the helper cell lines described above that express viral proteins constitutively, some helper cell lines have been designed to express the viral proteins in an inducible manner.
- One rationale for the generation of an inducible helper cell line is that some viral proteins are cytotoxic and cannot be easily expressed at high levels. By using an inducible system, expression of the cytotoxic proteins can be limited to the stage in which virus is propagated. By controlling the expression of the cytotoxic proteins, high viral titers can be achieved. Examples of the inducible helper cells include the 293GPG cells and HIV-1 helper cell lines.
- transient transfection systems have also been developed for propagation of retroviral vectors.
- helper functions are generally expressed from two different constructs, one expressing gag-pol and another expressing env. These two constructs generally share little sequence homology.
- the retroviral vector and the helper constructs are transfected into cells, and viruses are harvested a few days after transfection
- Pseudotyping refers to viral particles containing a viral genome from one virus and part (or all) of the viral proteins from a different virus.
- the most common form of pseudotyping involves one virus using the envelope protein of another virus.
- Some of the helper cell lines contain helper constructs that express gag-pol from one virus and env from another virus. Since the Gag polyproteins select the viral RNA, the viral vector to be propagated contains an RNA that is recognized by the Gag polyprotein expressed in these cells. However, the viral particles produced contain the Env protein derived from another virus. Therefore, these viral particles can only infect cells that express a receptor that can interact with the heterologous envelope protein.
- the helper cell line PG13 expresses gag-pol from MLV and env from gibbon ape leukemia virus (GaLV). Because the PG13 cell line expresses MLV Gag polyprotein, it can efficiently package MLV-based retroviral vectors. It has also been shown that some envelopes derived from viruses of a different family can also pseudotype retroviruses and generate infectious viral particles. For example, the G protein of vesicular stomatitis virus (VSV), a rhabdovirus, can be used to generate pseudotyped retroviral vectors. These VSV G pseudotyped viruses exhibit a very broad host range and can infect a variety of cells that cannot normally be infected with retroviruses.
- VSV vesicular stomatitis virus
- envelopes that can be used for vector pseudotyping are those of the following viruses: the RD114 endogenous feline retrovirus, which effectively targets hematopoietic cells, the Lymphocytic ChorioMeningitis Virus (LCMV), the Rabies virus, the Ebola and Mokola viruses, the Ross River and Semliki Forest virus, and the baculovirus gp64 envelope.
- LCMV Lymphocytic ChorioMeningitis Virus
- Rabies virus the Rabies virus
- Ebola and Mokola viruses the Ross River and Semliki Forest virus
- the baculovirus gp64 envelope the baculovirus gp64 envelope.
- Pseudotyping may involve for example a retroviral genome based on a lentivirus such as an HIV or equine infectious anaemia virus (EIAV) and the envelope protein may for example be the amphotropic envelope protein designated 4070A.
- envelope protein may be a protein from another virus such as an Influenza haemagglutinin.
- the envelope protein may be a modified envelope protein such as a mutant, truncated or engineered envelope protein (such as the engineered RD114 envelope). Modifications may be made or selected to introduce targeting ability or to reduce toxicity or for another purpose.
- retroviral vectors Some recently developed systems use a hybrid approach for propagation of retroviral vectors.
- a helper cell line is used to constitutively express some of the viral proteins, whereas other viral proteins are introduced into the helper cell line by transient transfection.
- a retroviral vector can be introduced into a helper cell line that constitutively expresses the MLV gag-pol.
- a plasmid designed to express the VSV G can be introduced into the system by transient transfection.
- the retroviral vector itself may encode some of the viral proteins (for example, Gag/Gag-Pol), and a helper cell line may provide other viral proteins (Env) (Boerkoel et al., 1993).
- a modified herpes simplex virus was generated to contain the retroviral gag, pol, and env to serve the helper function.
- adenovirus vectors and Semliki Forest virus-derived expression vectors have also been used to deliver genes encoding MLV viral proteins to helper cells.
- Retroviruses have been modified to generate vectors that can carry gene(s) of interest (transgene).
- Viral vectors generally contain all of the cis-acting elements needed for viral replication and gene expression. Additional elements may also be needed in vectors derived from some viruses to ensure successful gene delivery. The requirement for these cis-acting elements has often become apparent from greater understanding of the biology of these viruses.
- retroviral vectors are in plasmid form and have a backbone containing the bacterial origin of replication and an antibiotic resistance gene. The following steps are typically carried out to produce viral particles from retroviral vectors.
- Vector DNA is first introduced into the helper cells by transfection, electroporation, or lipofection.
- the vector DNA After introduction of the DNA into the helper cells, the vector DNA integrates into the helper cell and is expressed.
- the viral RNA is expressed from the 5′LTR and consists of all the sequences between the two R regions. This viral RNA contains the packaging signal and is packaged into the viral particles efficiently.
- the plasmid backbone sequences outside the two LTRs are not transferred to the target cells. The basic structures of some retroviral vectors derived from different retroviruses are described below.
- Vectors derived from three different oncoviruses will be described here to represent some of the most widely used retroviral vectors.
- Oncoviruses can only infect dividing cells; therefore, vectors that are derived from oncoviruses can only be used to efficiently deliver genes into dividing cells.
- the requirement for cell proliferation can sometimes be used as an advantage to selectively target rapidly dividing cells (for example, cancer cells).
- the vectors contain cis-acting viral sequences that are needed for gene expression and viral replication such as LTRs, PBS, PPT, and att.
- the packaging signal can be a minimum signal or a longer signal that extends into the gag open reading frame (+). When the + is present in the vector, it is necessary to mutate the translational initiation codon of gag to prevent expression of the truncated Gag protein.
- Several vectors have been designed to contain multiple restriction enzyme sites between the packaging signal and the 3′ untranslated region. The presence of these cloning sites facilitates the construction of vectors that can express the gene of interest.
- MLV-based vectors can be propagated in all of the MLV helper cell lines efficiently.
- MLV envelope proteins that dictate the host range of MLV vectors. Viruses that use the ecotropic envelope can infect mouse cells but not cells derived from other species. Viruses that use the amphotropic envelope can infect both mouse cells and cells derived from other species, including human cells. Viruses that use the xenotropic envelope cannot infect mouse cells but can infect cells derived from other species.
- MLV vectors can also be propagated in spleen necrosis virus (SNV)-based helper cell lines. SNV is an avian virus that is distantly related to MLV. Surprisingly, SNV proteins retain the ability to interact with MLV cis-acting sequences and package MLV RNA, reverse transcribe the MLV genome, and integrate the MLV RNA into the host.
- SNV spleen necrosis virus
- Spleen Necrosis Virus-Based Vectors The required viral sequences in these vectors are very similar to those of the MLV vectors.
- the packaging signal of SNV, denoted E, does not extend into the gag open reading frame; therefore, most SNV-based vectors do not contain the gag coding regions.
- the genes of interest are inserted into a linker region containing multiple restriction sites between the packaging signal and the 3′ untranslated region.
- SNV-based vectors can be propagated in SNV-based helper cell lines such as C3A2, DSDH, DSH134G, and DSN.
- RSV Rous Sarcoma Virus- and Avian Leukosis Virus-Based Vectors.
- RSV is the only known acute oncogenic retrovirus that is replication-competent.
- RSV also encodes the oncogene v-src between env and the 3′ LTR.
- a splice acceptor site upstream of the v-src allows the gene to be expressed as a spliced.
- RSV has the ability to code for an additional gene.
- Various modifications have been made to generate a replication-competent viral vector, an example of which is the replacement of v-src by a splice acceptor site and several restriction enzyme sites. DNA fragments can be inserted in the restriction sites to generate a replication-competent vector that expresses the gene of interest.
- ALV has also been modified to generate vectors that require helper cells for their propagation. Similar to the MLV and SNV vectors described above, the basic structure of an ALV vector also contains the 5′ and 3′ LTRs, att, PBS, PPT, and a packaging signal.
- the packaging signal of ALV extends into the gag open reading frame, and the relevant portions of gag are included in ALV-based vectors to achieve efficient packaging.
- lentivirus-based vectors In contrast to the oncoviruses, some lentiviruses have been shown to infect nondividing, quiescent cells. Lentiviruses are complex retroviruses that may need to express accessory proteins for regulation of their replication cycle. Some of these accessory proteins bind to regions of the viral genome to regulate gene expression. Therefore, lentivirus-based vectors need to incorporate additional cis-acting elements so that efficient viral replication and gene expression can occur. As examples of lentivirus-based vectors, HIV-1- and HIV-2-based vectors are described below.
- the HIV-1 vector contains cis-acting elements that are also found in simple retroviruses. It has been shown that sequences that extend into the gag open reading frame are important for packaging of HIV-1. Therefore, HIV-1 vectors often contain the relevant portion of gag in which the translational initiation codon has been mutated. In addition, most HIV-1 vectors also contain a portion of the env gene that includes the RRE. Rev binds to RRE, which permits the transport of full-length or singly spliced mRNAs from the nucleus to the cytoplasm. In the absence of Rev and/or RRE, full-length HIV-1 RNAs accumulate in the nucleus.
- a constitutive transport element from certain simple retroviruses such as Mason-Pfizer monkey virus can be used to relieve the requirement for Rev and RRE.
- Efficient transcription from the HIV-1 LTR promoter requires the viral protein Tat. Therefore, it is important that Tat is expressed in target cells if efficient transcription from the HIV-1 LTR is needed.
- the need for Tat expression can be met by expressing the Tat gene from the retroviral vector.
- expressing the gene of interest from a heterologous internal promoter can circumvent the need for Tat expression.
- HIV-2-based vectors are structurally very similar to HIV-1 vectors. Similar to HIV-1-based vectors, HIV-2 vectors also require RRE for efficient transport of the full-length or singly spliced viral RNAs.
- the HIV-1 vector can be propagated to high viral titers using viral proteins from simian immunodeficiency virus.
- the vector and helper constructs are from two different viruses, and the reduced nucleotide homology may decrease the probability of recombination.
- vectors based on feline immunodeficiency virus have also been developed as an alternative to vectors derived from the pathogenic HIV-1 genome. The structures of these vectors are also similar to the HIV-1 based vectors.
- Foamy viruses are unconventional retroviruses in that many features in their replication cycle are different from those of oncoviruses and lentiviruses. Although these viruses can be toxic to cultured cells, none of the foamy viruses are known to cause any disease in hosts.
- foamy virus vector contains the typical retroviral cis-acting sequences.
- sequences in the 5′ untranslated region the 5′ portion of the gag open reading frame and sequences in the 3′ portion of the pol open reading frame are important for efficient packaging. Similar to the lentiviruses, expression from the human foamy virus promoter is activated by the viral protein Tas.
- Retroviral vectors may contain many different modifications that serve various purposes for the gene therapist. These modifications may be introduced to permit the expression of more than one gene, regulate gene expression, activate or inactivate the viral vectors, and eliminate viral sequences to avoid generation of a replication-competent virus. Some examples of these modifications are described below.
- U3 Promoter-Driven Gene Expression Full-length viral RNA is expressed from the retroviral promoter located in the U3 region of the 5′ LTR.
- the viral RNA contains the R, U5, 5′ untranslated region, a gene of interest, 3′ untranslated region, U3, and R.
- the gene inserted between the 5′ and 3′ untranslated regions can be translated from the full-length RNA that is transcribed from the U3 promoter.
- helper cells transfected or infected by the viral vectors can be selected. Therefore, it is often necessary to design retroviral vectors that express a selectable marker gene as well as a gene of interest.
- Drug resistance genes are frequently used as selectable markers, but other marker genes, such as the green fluorescent protein gene, can also be used to select for transfected or infected cells.
- the expression of two genes in a retroviral vector can be achieved by expressing the 3′ gene by using an internal promoter, RNA splicing, or an internal ribosomal entry site (IRES).
- RNA that is expressed from the viral U3 promoter is used to translate a first gene of interest(s).
- the subgenomic RNA that is expressed from the internal promoter is used to translate a second gene of interest(s).
- Retroviruses express env by regulated splicing.
- the splice donor site that is used to express env is located in the 5′ untranslated region of retroviruses.
- some full-length viral RNAs are spliced to produce subgenomic viral RNAs that are used to express the Env proteins.
- Splicing vectors were developed by using the same principle to express two different genes by using the viral splice donor and splice acceptor sites. The advantage of splicing vectors is that only one promoter is necessary, and any potential for promoter interference is eliminated.
- sequences allowing expression of multiple proteins from a single transcript are self-cleaving 2A-like peptides (also called CHYSEL, cis-acting hydrolase elements) derived from the Foot-and-Mouth disease virus and other picoRNA viruses.
- CHYSEL self-cleaving 2A-like peptides
- cis-acting hydrolase elements derived from the Foot-and-Mouth disease virus and other picoRNA viruses.
- bidirectional promoters can be used to express two genes from the same promoter.
- the LTR sequences are duplicated in retroviral vectors has been exploited to construct vectors containing two copies of the gene of interest.
- the first set of double-copy vectors contains the gene of interest in the U3 region upstream of the viral. These genes are expressed using either an RNA polymerase II promoter or an RNA polymerase III promoter. This strategy has been shown to successfully increase the level of gene expression.
- the vector contains the gene of interest in the middle of the R region.
- a replication-competent virus can be generated during propagation of the vectors, which can lead to inadvertent spread of the therapeutic vector to nontarget tissues.
- a class of vectors was designed to undergo self-inactivation. The principle is that after gene delivery, the vector will delete some of the cis-acting elements needed to complete another round of replication. Therefore, even in the presence of a replication-competent virus, these vectors cannot be transferred to other target cells efficiently.
- the generation of a replication-competent virus sometimes involves recombination between the defective helper plasmid and the vector encoding the gene of interest. Therefore, another possible benefit of the self-inactivating vector is that it may decrease the probability of generating a replication-competent virus.
- U3 Minus Vectors were the first self-inactivating retroviral vectors to be developed. These vectors are designed to delete the viral U3 promoter during reverse transcription so that the provirus in the target cell lacks a viral promoter. In these vectors, the U3 of the 5′ LTR is intact, whereas the U3 of the 3′ LTR is inactivated by a large deletion.
- the RNA generated from this vector contains R, U5, 5′ untranslated region, gene(s) of interest, 3′ untranslated region, a deleted U3, and R. During reverse transcription, the U3 at the 3′ end of the viral RNA is normally used as a template to generate the LTR.
- the viral DNA that is synthesized from the U3 minus vector through reverse transcription contains deleted U3 sequences in both LTRs. Since the viral promoter is deleted during reverse transcription, the gene of interest is under the control of an internal promoter.
- the advantage of the U3 minus vector is that it is potentially safer, since the probability of generation of a replication-competent virus is reduced. However, at a low frequency, recombination during DNA transfection can occur to regenerate the U3 at the 3′ LTR. If this occurs, the resulting vector will still contain the promoter in the U3 and thus retain two complete LTRs. Additional modifications have been made in some U3 minus vectors to decrease the homology between the 5′ and 3′ LTRs, which reduces the probability of recombination and regeneration of an intact LTR during DNA transfection.
- Cre/loxP Vectors The Cre recombinase, a naturally occurring site-specific recombinase of bacteriophage P1, recognizes a 32-bp sequence named loxP. Cre can efficiently mediate site-specific recombination using two loxP sites separated by sequences of variable lengths. The recombination events include deletion, insertion, and inversion of the sequences between the loxP sites. This system has been exploited to develop self-inactivating retroviral vectors (Choulika et al., 1996; Russ et al., 1996). An example of such a vector contains an intact 5′ LTR and all of the cis-acting elements needed for retroviral replication.
- the vector contains the cre recombinase gene that is expressed using an internal promoter.
- the 3′ LTR has been modified by insertion of several sequences in the U3, including a loxP site, a promoter, and a gene of interest; in addition, the 3′ U3 often contains a deletion to reduce the promoter activity.
- the full-length viral RNA is packaged into virion, and upon infection of target cells, the viral RNA is reverse-transcribed.
- the 3′ U3 sequence is used as a template to synthesize both LTRs; consequently, the sequences in both LTRs contain a copy of the loxP site, a promoter, and a gene of interest.
- the cre gene is expressed, and the Cre recombinase is synthesized in the infected target cells.
- the Cre recombinase then mediates the deletion of sequences between the two loxP sites in the viral DNA, which results in deletion of the 5′ LTR, the 5′ untranslated region, the internal promoter, and cre.
- the provirus in the target cells contains only one LTR that expresses the gene of interest.
- the Cre/loxP system can be used to delete different sequences in the retroviral vector as well as delete portions of the helper construct in the packaging cells.
- Another application of the Cre/loxP system is that it can be used to delete the selectable marker from a retroviral vector after the viral DNA is integrated into the chromosome of the target cells.
- the selectable marker is included in the vector so that helper cells transfected with the vector DNA can be selected. Deletion of the selectable marker is desirable because the presence of the selectable marker can lead to promoter interference or an immune response against the transduced cells. Deletion of the selectable marker is accomplished by insertion of two loxP sites that flank the selectable marker gene.
- the target cells After the vector is introduced into target cells by infection, the target cells are infected with another vector that expresses the Cre recombinase.
- the Cre recombinase then deletes sequences between the two loxP sites, which include the selectable marker. As a result, the final provirus expresses only the gene of interest.
- helper cells it may be desirable to have an inactivated gene of interest in the helper cells and activate this gene after it is delivered to target cells.
- the product from the gene of interest is cytotoxic
- expressing the gene in helper cells would result in toxicity and most likely reduce or eliminate viral production.
- a series of vectors have been generated to simultaneously activate a gene and inactivate the vector during gene delivery. This is accomplished by the frequent deletion of directly repeated sequences during reverse transcription. If directly repeated sequences are present in a virus, one copy of the direct repeat and all of the sequences between the two repeats can be deleted at high frequencies during reverse transcription. This property of reverse transcriptases has been exploited to generate the self-activating and self-inactivating retroviral vectors.
- An important goal for gene therapists is to develop a means to target gene delivery to specific cell types or tissues.
- At least two strategies have been used in an effort to target gene delivery using retroviral vectors.
- One strategy is designed to control gene delivery at the point of virus entry into the host cell by using natural or genetically engineered envelope proteins that interact with cell-type-specific receptors.
- Another strategy is designed to control expression of the therapeutic gene in specific cell types by using tissue-specific promoters.
- Promoters that are active in certain tissues or respond to certain reagents can be used to regulate the expression of a gene of interest. These promoters can be inserted between the LTRs of a retroviral vector. Alternatively, the regulated promoter can be used to replace the viral promoter in the U3 region.
- the design of a retroviral vector with an internal tissue-specific promoter is similar to that of other retroviral vectors containing internal promoters.
- the retroviral vector particle according to the invention will also be capable of transducing cells which are slowly-dividing, and which non-lentiviruses such as MLV would not be able to efficiently transduce.
- Slowly-dividing cells divide once in about every three to four days including certain tumour cells.
- tumours contain rapidly dividing cells, some tumour cells especially those in the centre of the tumour, divide infrequently.
- the target cell may be a growth-arrested cell capable of undergoing cell division such as a cell in a central portion of a tumour mass or a stem cell such as a haematopoietic stem cell or a CD34-positive cell.
- the target cell may be a precursor of a differentiated cell such as a monocyte precursor, a CD33-positive cell, or a myeloid precursor.
- the target cell may be a differentiated cell such as a neuron, astrocyte, glial cell, microglial cell, macrophage, monocyte, epithelial cell, endothelial cell or hepatocyte.
- Target cells may be transduced either in vitro after isolation from a human individual or may be transduced directly in vivo.
- the adenovirus is a double-stranded, linear DNA virus that does not go through an RNA intermediate.
- RNA intermediate There are over 50 different human serotypes of adenovirus divided into 6 subgroups based on the genetic sequence homology all of which exhibit comparable genetic organisation.
- Human adenovirus group C serotypes 2 and 5 (with 95% sequence homology) are most commonly used in adenoviral vector systems and are normally associated with upper respiratory tract infections in the young.
- the adenoviruses/adenoviral vectors of the invention may be of human or animal origin.
- preferred adenoviruses are those classified in group C, in particular the adenoviruses of type 2 (Ad2), 5 (Ad5), 7 (Ad7) or 12 (Ad12). More preferably, it is an Ad2 or Ad5 adenovirus.
- Ad2 or Ad5 adenovirus canine adenovirus, mouse adenovirus or an avian adenovirus such as CELO virus (Cotton et al., 1993, J Virol 67:3777-3785) may be used.
- adenoviruses of canine origin and especially the strains of the CAV2 adenoviruses [manhattan strain or A26/61 (ATCC VR-800) for example].
- Other adenoviruses of animal origin include those cited in application WO-A-94/26914 incorporated herein by reference.
- the genome of adenoviruses comprises an inverted terminal repeat (ITR) at each end, an encapsidation sequence (Psi), early genes and late genes.
- ITR inverted terminal repeat
- Psi encapsidation sequence
- the main early genes have been classified into an array of intermediate early (E1a), delayed early (E1b, E2a, E2b, E3 and E4), and intermediate regions. Among these, the genes contained in the E1 region in particular are necessary for viral propagation.
- the main late genes are contained in the L1 to L5 regions.
- Ad5 adenovirus The genome of the Ad5 adenovirus has been completely sequenced and is available on a database (see particularly Genbank Accession No. M73260). Likewise, parts, or even all of other adenoviral genomes (such as Ad2, Ad7, Ad12) have also been sequenced.
- an adenovirus is typically modified so as to make it incapable of replicating in an infected cell.
- constructs described in the prior art include adenoviruses deleted for the E1 region, essential for viral replication, into which are inserted the heterologous DNA sequences (Levrero et al., 1991, Gene 101: 195; Gosh-Choudhury et al., 1986, Gene 50: 161).
- a heat-sensitive point mutation has been introduced into the ts125 mutant, making it possible to inactivate the 72 kDa DNA-binding protein (DBP).
- a recombinant adenoviral vector used in the invention comprises a deletion in the E1 region of its genome.
- the E1 region comprises a deletion in the E1a and E1b regions.
- the E1 region is inactivated by deletion of a PvuII-Bg1II fragment stretching from nucleotide 454 to nucleotide 3328, in the Ad5 adenovirus sequence (Genbank Accession No. M73260).
- the E1 region is inactivated by deletion of an HinfII-Sau3A fragment stretching from nucleotide 382 to nucleotide 3446.
- adenoviral vectors comprise a deletion of another region essential for viral replication and/or propagation, the E4 region.
- the E4 region is involved in the regulation of the expression of the late genes, in the stability of the late nuclear RNAs, in decreasing host cell protein expression and in the efficiency of the replication of the viral DNA.
- Adenoviral vectors in which the E1 and E4 regions are deleted therefore possess very reduced viral gene expression and transcriptional background noise.
- Such vectors have been described for example in applications WO-A-94/28152, WO-A-95/02697, WO-A-96/22378.
- vectors carrying a modification of the IVa2 gene have also been described (WO-A-96/10088).
- a recombinant adenoviral vector used in the invention comprises, in addition, a deletion in the E4 region of its genome. More particularly, the deletion in the E4 region affects all the open reading frames. There may be mentioned, by way of a precise example, deletions of nucleotides 33466-35535 or 33093-35535.
- preferred vectors comprise a deletion of the whole of the E4 region. This may be carried deletion or excision of an MaeII-MscI fragment corresponding to nucleotides 35835-32720. Other types of deletions in the E4 region are described in applications WO-A-95/02697 and WO-A-96/22378, incorporated herein by reference.
- E4 only a functional part of E4 is deleted.
- This part comprises at least the ORF3 and ORF6 frames.
- these coding frames can be deleted from the genome in the form of PvuII-AluI and BglII-PvuII fragments respectively, corresponding to nucleotides 34801-34329 and 34115-33126 respectively.
- the deletions of the E4 region of the virus Ad2 d1808 or of viruses Ad5 d11004, Ad5 d11007, Ad5 d11011 or Ad5 d11014 can also be used within the framework of the invention.
- positions given above refer to the wild-type Ad5 adenovirus sequence as published and accessible on a database. Although minor variations may exist between the various adenovirus serotypes, these positions are generally applicable to the construction of recombinant adenoviruses according to the invention from any serotype, and especially the adenoviruses Ad2 and Ad7.
- the adenoviruses produced may possess other alterations in their genome.
- other regions may be deleted to increase the capacity of the virus and reduce its side effects linked to the expression of viral genes.
- all or part of the E3 or IVa2 region in particular may be deleted.
- the E3 region it may however be particularly preferred to conserve the part encoding the gp19K protein. This protein indeed makes it possible to prevent the adenoviral vector from becoming the subject of an immune reaction which (i) would limit its action and (ii) could have undesirable side effects.
- the E3 region is deleted and the sequence encoding the gp19K protein is reintroduced under the control of a heterologous promoter.
- the polynucleotide of the invention/NOI can be inserted into various sites of the recombinant genome. It can be inserted at into the E1, E3 or E4 region, as a replacement for the deleted or surplus sequences. It can also be inserted into any other site, outside the sequences necessary in cis for the production of the viruses (ITR sequences and encapsidation sequence).
- the E2 region is essential as it encodes the 72 kDa DNA binding protein, DNA polymerase and the 80 kDa precurser of the 55 kDa Terminal Protein (TP) needed for protein priming to initiate DNA synthesis.
- TP Terminal Protein
- the recombinant adenoviruses are typically produced in an encapsidation cell line, which is a cell line capable of complementing in trans one or more of the functions deficient in the recombinant adenoviral genome.
- an encapsidation cell line which is a cell line capable of complementing in trans one or more of the functions deficient in the recombinant adenoviral genome.
- One of these lines is for example line 293, into which part of the adenovirus genome has been integrated.
- line 293 is a human kidney embryonic cell line containing the left end (about 11-12%) of the genome of serotype 5 adenovirus (Ad5), comprising the left ITR, the encapsidation region, the E1 region, including E1a and E1b, the region encoding protein pIX and part of the region encoding protein pIVa2.
- This line is capable of transcomplementing recombinant adenoviruses defective for the E1 region, that is to say lacking all or part of the E1 region, and of producing viral stocks having high titres.
- This line is also capable of producing, at a permissive temperature (32° C.), virus stocks comprising, in addition, the heat-sensitive E2 mutation.
- cell lines capable of complementing the E1 region have been described, based in particular on human lung carcinoma cells A549 (WO-A-94/28152) or on human retinoblasts (Hum. Gen. Ther. (1996) 215).
- cell lines capable of transcomplementing several adenovirus functions have also been described, for example cell lines complementing the E1 and E4 regions (Yeh et al., 1996, J. Virol. 70: 559; Krougliak et al., 1995, Hum. Gen. Ther. 6: 1575) and lines complementing the E1 and E2 regions (WO-A-94/28152, WO-A-95/02697, WO-A-95/27071).
- the recombinant adenoviruses are usually produced by introducing the viral DNA into the encapsidation line, followed by lysis of the cells after about 2 or 3 days (the kinetics of the adenoviral cycle being 24 to 36 hours).
- the viral DNA introduced may be the complete recombinant viral genome, optionally constructed in a bacterium (WO-A-96/25506) or in a yeast (WO-A-95/03400), transfected into the cells. It may also be a recombinant virus used to infect the encapsidation line.
- the viral DNA may also be introduced in the form of fragments each carrying part of the recombinant viral genome and a region of homology which makes it possible, after introduction into the encapsidation cell, to reconstitute the recombinant viral genome by homologous recombination between the various fragments.
- Replication-competent adenoviruses can also be used for gene therapy.
- the E1a gene can be inserted into a first generation virus under the regulation of a tumour-specific promoter.
- This type of vector could be used either to kill tumour cells directly by lysis or to deliver a “suicide gene” such as the herpes-simplex-virus thymidine-kinase gene (HSV tk) which can kill infected and bystander cells following treatment with ganciclovir.
- a “suicide gene” such as the herpes-simplex-virus thymidine-kinase gene (HSV tk) which can kill infected and bystander cells following treatment with ganciclovir.
- the present invention provides an adenovirus vector comprising a polynucleotide of the invention operably linked to a nucleic acid sequence encoding an adenoviral E1a polypeptide.
- the E1a polypeptide under the control of the HRE enhancer would only be expressed under hypoxic conditions and therefore the adenovirus would only be replication competent under hypoxic conditions.
- the adenovirus lacks an endogenous E1 gene, and preferably also lacks an endogenous E3 gene. Other regions of the adenovirus genome which may be deleted are described above. It may also be desirable to include all or part of the E3 gene under the control of a hypoxia response element such that host cell immune modulation is balances to obtain the correct viral spread within the tumour and immune response to infected cells.
- E1b An adenovirus defective only for E1b has been used specifically for antitumour treatment in phase-1 clinical trials.
- the polypeptides encoded by E1b are able to block p53-mediated apoptosis, preventing the cell from killing itself in response to viral infection.
- the virus in normal non tumour cells, in the absence of E1b, the virus is unable to block apoptosis and is thus unable to produce infectious virus and spread.
- tumour cells deficient in p53 the E1b defective virus can grow and spread to adjacent p53-defective tumour cells but not to normal cells. Again, this type of vector could also be used to deliver a therapeutic gene such as HSV tk.
- the E1a-expressing adenoviruses of the present invention lack a functional E1b gene.
- the HSV vectors of the invention may be derived from, for example, HSV1 or HSV2 strains, or derivatives thereof, preferably HSV1.
- Derivatives include inter-type recombinants containing DNA from HSV1 and HSV2 strains. Derivatives preferably have at least 70% sequence homology to either the HSV1 or HSV2 genomes, more preferably at least 90%, even more preferably 95%.
- HSV vectors are to be used for gene therapy in humans the polynucleotide should preferably be inserted into an essential gene. This is because if a vector virus encounters a wild-type virus transfer of a heterologous gene to the wild-type virus could occur by recombination. However as long as the polynucleotide is inserted into an essential gene this recombinational transfer would also delete the essential gene in the recipient virus and prevent ‘escape’ of the heterologous gene into the replication competent wild-type virus population.
- Attenuated strains may be used to produce the HSV strain of the present invention, here given as examples only, including strains that have mutations in either ICP34.5 or ICP27, for example strain 1716 (MacLean et al., 1991, J Gen Virol 72: 632-639), strains R3616 and R4009 (Chou and Roizman, 1992, PNAS 89: 3266-3270) and R930 (Chou et al., 1994, J. Virol 68: 8304-8311) all of which have mutations in ICP34.5, and d27-1 (Rice and Knipe, 1990, J. Virol 64: 1704-1715) which has a deletion in ICP27.
- strains deleted for ICP4, ICP0, ICP22, ICP6, ICP47, vhs or gH, with an inactivating mutation in VMW65, or with any combination of the above may also be used to produce HSV strains of the invention.
- HSV genes The terminology used in describing the various HSV genes is as found in Coffin and Latchman, 1996. Herpes simplex virus-based vectors. In: Latchman DS (ed). Genetic manipulation of the nervous system. Academic Press: London, pp 99-114.
- ICP27-expressing cell lines can be produced by co-transfecting mammalian cells, for example the Vero or BHK cells, with a vector, preferably a plasmid vector, comprising a functional HSV ICP27 gene capable of being expressed in said cells, and a vector, preferably a plasmid vector, encoding a selectable marker, for example neomycin resistance.
- Clones possessing the selectable marker are then screened further to determine which clones also express functional ICP27, for example on the basis of their ability to support the growth of ICP27 ⁇ mutant HSV strains, using methods known to those skilled in the art (for example as described in Rice and Knipe, 1990).
- HSV strains of the invention comprise inactivating modifications in other essential genes, for example ICP4, complementing cell lines will further comprise a functional HSV gene which complements the modified essential gene in the same manner as described for ICP27.
- HSV genes may be rendered functionally inactive by several techniques well known in the art. For example, they may be rendered functionally inactive by deletions, substitutions or insertions, preferably by deletion. Deletions may remove portions of the genes or the entire gene. Inserted sequences may include the expression cassette described above.
- HSV genomic DNA is transfected together with a vector, preferably a plasmid vector, comprising the mutated sequence flanked by homologous HSV sequences.
- the mutated sequence may comprise deletions, insertions or substitutions, all of which may be constructed by routine techniques. Insertions may include selectable marker genes, for example lacZ, for screening recombinant viruses by, for example, ⁇ -galactosidase activity.
- Mutations may also be made in other HSV genes, for example genes such as ICP0, ICP4, ICP6, ICP22, ICP47, VMW65, gH or vhs.
- VMW65 gene
- the entire gene is not deleted since it encodes an essential structural protein, but a small inactivating insertion is made which abolishes the ability of VMW65 to transcriptionally activate IE genes (Ace et al., 1989, J Virol 63: 2260-2269).
- HSV Strains Comprising a Transgene and miRNA of the Invention
- a transgene and mircoRNA of the invention may be inserted into the HSV genome at any location provided that the virus can still be propagated, which may require the use of a cell line carrying another HSV essential gene (as described in 2.) if the NOI is inserted into an essential gene
- sequences of the invention may be inserted into the HSV genome by homologous recombination of HSV strains with, for example, plasmid vectors carrying the expression cassette flanked by HSV sequences, as described above for introducing mutations.
- the polynucleotide may be introduced into a suitable plasmid vector comprising HSV sequences using cloning techniques well-known in the art.
- the miRNA and transgene may be administered to a patient or used to produce a transgenic plant or non-human animal.
- the term “administered” includes delivery by viral or non-viral techniques.
- Viral delivery mechanisms include but are not limited to adenoviral vectors, adeno-associated viral (AAV) vectors, herpes viral vectors, retroviral vectors, lentiviral vectors, and baculoviral vectors etc as described above.
- Non-viral delivery mechanisms include lipid mediated transfection, liposomes, immunoliposomes, lipofectin, cationic facial amphiphiles (CFAs) and combinations thereof.
- the delivery of one or more therapeutic genes by a vector system according to the present invention may be used alone or in combination with other treatments or components of the treatment.
- the vector of the present invention may be used to deliver one or more transgene(s) useful in the treatment of the disorders listed in WO-A-98/05635.
- cancer inflammation or inflammatory disease
- dermatological disorders fever, cardiovascular effects, haemorrhage, coagulation and acute phase response, cachexia, anorexia, acute infection, HIV infection, shock states, graft-versus-host reactions, autoimmune disease, reperfusion injury, meningitis, migraine and aspirin-dependent anti-thrombosis
- cerebral ischaemia ischaemic heart disease, osteoarthritis, rheumatoid arthritis, osteoporosis, asthma, multiple sclerosis, neurodegeneration, Alzheimer's disease, atherosclerosis, stroke, vasculitis, Crohn's disease and ulcerative colitis; periodontitis, gingivitis; p
- the vector of the present invention may be used to deliver one or more transgene(s) useful in the treatment of disorders listed in WO-A-98/07859.
- cytokine and cell proliferation/differentiation activity e.g. for treating immune deficiency, including infection with human immune deficiency virus; regulation of lymphocyte growth; treating cancer and many autoimmune diseases, and to prevent transplant rejection or induce tumour immunity
- regulation of haematopoiesis e.g. treatment of myeloid or lymphoid diseases
- promoting growth of bone, cartilage, tendon, ligament and nerve tissue e.g.
- follicle-stimulating hormone for healing wounds, treatment of burns, ulcers and periodontal disease and neurodegeneration; inhibition or activation of follicle-stimulating hormone (modulation of fertility); chemotactic/chemokinetic activity (e.g. for mobilising specific cell types to sites of injury or infection); haemostatic and thrombolytic activity (e.g. for treating haemophilia and stroke); antiinflammatory activity (for treating e.g. septic shock or Crohn's disease); as antimicrobials; modulators of e.g. metabolism or behaviour; as analgesics; treating specific deficiency disorders; in treatment of e.g. psoriasis, in human or veterinary medicine.
- the retroviral vector of the present invention may be used to deliver one or more transgenes(s) useful in the treatment of disorders listed in WO-A-98/09985.
- transgenes(s) useful in the treatment of disorders listed in WO-A-98/09985.
- macrophage inhibitory and/or T cell inhibitory activity and thus, anti-inflammatory activity i.e.
- inhibitory effects against a cellular and/or humoral immune response including a response not associated with inflammation; inhibit the ability of macrophages and T cells to adhere to extracellular matrix components and fibronectin, as well as up-regulated fas receptor expression in T cells; inhibit unwanted immune reaction and inflammation including arthritis, including rheumatoid arthritis, inflammation associated with hypersensitivity, allergic reactions, asthma, systemic lupus erythematosus, collagen diseases and other autoimmune diseases, inflammation associated with atherosclerosis, arteriosclerosis, atherosclerotic heart disease, reperfusion injury, cardiac arrest, myocardial infarction, vascular inflammatory disorders, respiratory distress syndrome or other cardiopulmonary diseases, inflammation associated with peptic ulcer, ulcerative colitis and other diseases of the gastrointestinal tract, hepatic fibrosis, liver cirrhosis or other hepatic diseases, thyroiditis or other glandular diseases, glomerulonephritis or other renal and urologic diseases, otitis or other oto-rhino-
- retinitis or cystoid macular oedema retinitis or cystoid macular oedema, sympathetic ophthalmia, scleritis, retinitis pigmentosa, immune and inflammatory components of degenerative fondus disease, inflammatory components of ocular trauma, ocular inflammation caused by infection, proliferative vitreo-retinopathies, acute ischaemic optic neuropathy, excessive scarring, e.g.
- monocyte or leukocyte proliferative diseases e.g. leukaemia
- monocytes or lymphocytes for the prevention and/or treatment of graft rejection in cases of transplantation of natural or artificial cells, tissue and organs such as cornea, bone marrow, organs, lenses, pacemakers, natural or artificial skin tissue.
- the present invention also provides a pharmaceutical composition for treating an individual by gene therapy, wherein the composition comprises a therapeutically effective amount of the vector of the present invention comprising one or more deliverable therapeutic and/or diagnostic transgenes(s) or a viral particle produced by or obtained from same.
- the pharmaceutical composition may be for human or animal usage. Typically, a physician will determine the actual dosage which will be most suitable for an individual subject and it will vary with the age, weight and response of the particular individual.
- the composition may optionally comprise a pharmaceutically acceptable carrier, diluent, excipient or adjuvant.
- a pharmaceutically acceptable carrier diluent, excipient or adjuvant.
- the choice of pharmaceutical carrier, excipient or diluent can be selected with regard to the intended route of administration and standard pharmaceutical practice.
- the pharmaceutical compositions may comprise as—or in addition to—the carrier, excipient or diluent any suitable binder(s), lubricant(s), suspending agent(s), coating agent(s), solubilising agent(s), and other carrier agents that may aid or increase the viral entry into the target site (such as for example a lipid delivery system).
- the pharmaceutical compositions can be administered by any one or more of: inhalation, in the form of a suppository or pessary, topically in the form of a lotion, solution, cream, ointment or dusting powder, by use of a skin patch, orally in the form of tablets containing excipients such as starch or lactose, or in capsules or ovules either alone or in admixture with excipients, or in the form of elixirs, solutions or suspensions containing flavouring or colouring agents, or they can be injected parenterally, for example intracavemosally, intravenously, intramuscularly or subcutaneously.
- compositions may be best used in the form of a sterile aqueous solution which may contain other substances, for example enough salts or monosaccharides to make the solution isotonic with blood.
- compositions may be administered in the form of tablets or lozenges which can be formulated in a conventional manner.
- the delivery of one or more therapeutic genes by a vector system according to the invention may be used alone or in combination with other treatments or components of the treatment.
- Diseases which may be treated include, but are not limited to: cancer, neurological diseases, inherited diseases, heart disease, stroke, arthritis, viral infections and diseases of the immune system.
- Suitable therapeutic genes include those coding for tumour suppressor proteins, enzymes, pro-drug activating enzymes, immunomodulatory molecules, antibodies, engineered immunoglobulin-like molecules, fusion proteins, hormones, membrane proteins, vasoactive proteins or peptides, cytokines, chemokines, anti-viral proteins, antisense RNA and ribozymes.
- miRNA Target (mirT) sequences were constructed as follows:
- mir-142-3p.Target For 4x.mir-142-3p.Target (mir-142-3pT) the following oligonucleotides were annealed: 5′CTAGAGTCGAC TCCATAAAGTAGGAAACACTACA CGAT TCCATAAAGT AGGAAACACTACA ACCGGT (S1) (SEQ ID NO: 1) 5′TGTAGTGTTTCCTACTTTATGGAATCGTGTAGTGTTTCCTACTTTATG GAGTCGACT (AS1) (SEQ ID NO: 2), 5′ TCCATAAAGTAGGAAACACTACA TCACT CCATAAAGTAGGAAACACTA CA C (S2) (SEQ ID NO: 3), 5′TCGAGTGTAGTGTTTCCTACTTTATGGAGTGATGTAGTGTTTCCTACT TTATGGAACCGGT (AS2) (SEQ ID NO: 4).
- oligonucleotides Ligation of these oligonucleotides created a doubled stranded DNA fragment with XbaI and XhoI sticky ends.
- the underlined sequences are designed to be perfectly complementary to a specific miRNA.
- the annealed oligonucleotides were subcloned into the XbaI and XhoI site of pBluescriptII.KS.
- the resulting vectors were subsequently digested with either SacII and KpnI, NheI and AgeI, or SalI, and the mirT fragment was isolated for ligation into the appropriate sites of the recipient vectors:
- VSV-pseudotyped third-generation LVs were produced by transient four-plasmid cotransfection into 293T cells and purified by ultracentrifugation as described (De Palma and Naldini, 2002). Expression titer of GFP was estimated on 293T cells by limiting dilution. Vector particles were measured by HIV-1 gag p24 antigen immunocapture (NEN Life Science Products). Concentrated vector expression titer ranged from 0.15 ⁇ 1.5 ⁇ 10 10 transducing units 293T (TU)/ml for all vectors.
- 293T cells were maintained in Iscove's modified Dulbecco's medium (IMDM; Sigma) supplemented with 10% fetal bovine serum (FBS; Gibco) and a combination of penicillin-streptomycin and glutamine.
- IMDM Iscove's modified Dulbecco's medium
- FBS fetal bovine serum
- the U937 monocyte cell line was maintained in RPMI supplemented as above (complete RPMI).
- Primary cultures of human dendritic cells were isolated from peripheral blood as previously described and maintained in complete RPMI supplemented with GM-CSF and IL-4 (Bender et al., 1996).
- RNA from cells and tissues was extracted by using “Blood & Cell Culture DNA Midi Kit” (Qiagen, Hilden, Germany), according to manufacturer's instructions.
- RNA from cells was extracted by using “Tri Reagent” (Sigma, Saint Louis, Mo.), according to manufacturer's instructions.
- Northern Blot was performed as previously described (De Palma and Naldini, 2002). Twenty micrograms of total RNA were loaded and 100 ng 32 P labelled-GFP probe was used.
- Vector C/G were quantified by real-time PCR, starting from 100 ng template DNA extracted from mouse tissues or 200 ng template DNA extracted from cell lines.
- the sets of primers and probe used for the analysis are the following:
- LV backbone 750 nmol forward primer (F): 5′TGAAAGCGAAAGGGAAACCA3′ (SEQ ID NO: 5), 200 nmol reverse primer (R): 5′-CCGTGCGCGCTTCAG-3′ (SEQ ID NO: 6), 200 nmol probe (P): 5′-VIC-CTCTCTCGACGCAGGACT-MGB-3′ (SEQ ID NO: 7); murine genomic DNA: ⁇ -actin: 300 nmol F: 5′-AGAGGGAAATCGTGCGTGAC-3′ (SEQ ID NO: 8), 750 nmol R: 5′-CAATAGTGATGACCTGGCCGT-3′ (SEQ ID NO: 9), 200 nmol P: 5′-VIC-CACTGCCGCATCCTCTTCCTCCC-MGB-3′ (SEQ ID NO: 10), human genomic DNA: hTERT: 200 nmol F: 5′-GGCACACGTGGCTTTTCG-3‘ (SEQ ID NO: 11), 600 nmol R:
- Reverse Transcription was carried out on 2 ⁇ g total RNA using the Random Hexamers protocol of the Superscript III First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, Calif.). Quantitative PCR analysis was performed to quantitate the concentration of GFP mRNA, and GAPDH expression was used for normalization. Two sets of primers and probe were used:
- F 5′-CAGCTCGCCGACCACTA-3′ (SEQ ID NO: 14), R: 5′-GGGCCGTCGCCGAT-3′ (SEQ ID NO: 15) and P: 5′-6FAM-CCAGCAGAACACCCCC-MGB-3′ (SEQ ID NO: 16), and for GAPDH: 200 nmol F: 5-ACCACAGTCCATGCCATCACT-3′, (SEQ ID NO: 17), 900 nmol R: 5′-GGCCATCACGCCACAGSTT-3′ (SEQ ID NO: 18) and 200 nmol P: 5′-TET-CCACCCAGAAGACTGTGGATGGCC-TAMRA-3′. (SEQ ID NO: 19).
- miRNA detection was carried out using the Applied Biosystems Taqman microRNA Assay system according to the manufacturer's instructions. Results were normalized to has-mir-16 and let-7a was used as a calibrator. Values are reported relative to the expression of let-7a.
- Transduced 293T cells were grown for at least 14 days before FACS analysis to reach steady state GFP expression and to rule out pseudotransduction.
- adherent cells were detached with 0.05% trypsin-EDTA, washed and resuspended in PBS containing 2% FBS.
- Cells grown in suspension were washed and resuspended in PBS containing 2% FBS.
- FBS FACS-containing 2% FBS.
- 10 5 cells were blocked in PBS, 5% human serum, 2% FBS for 15 min at 4° C.
- RPE R-phycoerythrin
- mice 6-8 week old Nude and Balb/c mice were purchased from Charles Rivers Laboratories (Milan, Italy) and maintained in specific-pathogen-free conditions.
- Hemophilia B (Clotting Factor IX knock-out) mice were a acquired from the Salk Institute (La Jolla, Calif.), and bred and maintained in specific-pathogen-free conditions.
- Vector administration was carried out by tail vein injection on mice. All animal procedures were performed according to protocols approved by the Hospital San Raffaele Institutional Animal Care and Use Committee.
- Transgenic mice were generated using LVs as described (Lois et al., 2002). Briefly, female FVB mice were superovulated with a combination of pregnant mare serum and human chorionic gonadotropin. On average between 20 and 30 embryos were collected per female and microinjected into the perivitelline space with 10-100 pl of 5 ⁇ 10 7 TU/ml LV stock on the same day. Manipulated embryos were immediately implanted into the oviduct of pseudopregnant CD1 mice. Pups were genotyped for the presence of the GFP sequence by PCR. Positive mice were bred to test germline transmission of the transgene. DNA was extracted from the tail and used to quantify vector copy number by real-time PCR in founder and F1 progeny mice.
- hF.IX concentration was determined in mouse citrated plasma by an enzyme-immunoassay for the factor IX:Ag (Roche, Milan, Italy), and for FIX activity by an activated partial thromboplastin time (aPTT) assay, as previously described (Brown et al., 2004b).
- LV miRNA-regulated lentiviral vector
- mir-142-3p and mir-142-5p are highly expressed in U937 cells, but detected only at low levels in 293T cells. mir-30a was found to be low in both 293T and U937 cells, and thus serves as a control for our studies.
- Vectors were prepared and concentrated as previously described. Titration of the miRNA-regulated LV indicated that inclusion of the target sequence did not adversely affect vector infectivity or the levels of transgene expression in non-hematopoeitic cells ( FIG. 2 b ). Levels of transduction and transgene expression were achieved that were comparable to the parent vector without the miRNA target sequence (LV.PGK.GFP). In contrast, transduction of both the human U937 monocyte cell line or human primary dendritic cells resulted in drastically different expression profiles between the two vectors, LV.PGK.GFP and LV.PGK.GFP.142-3pT.
- mir-30a is not expressed in hematopoietic cells (Zeng et al., 2002), and, as expected, we did not see any reduction in GFP expression following cell transduction.
- our results clearly demonstrate that in human cells our vector design maintains high vector infectivity, while preventing gene expression in particular cell types.
- Bd.LV bidirectional LV
- ⁇ LNGFR low-affinity nerve growth factor receptor
- the novelty of the miRNA regulation strategy provides the possibility to engineer vectors in manners not previously possible.
- miRNA regulation to selectively prevent transgene expression in vector producer cells.
- Normally during the process of vector production in addition to expression of the vector genome from the transfer plasmid, there is also expression of the transgene.
- this can be particularly problematic, since expression of the toxic protein kills the producer cells and leads to an overall reduction in vector titer.
- the ability to selectively prevent transgene expression in producer cells would be a major advancement for production of specific vectors, such as those encoding toxic molecules.
- mir-19a is highly expressed in 293T cells. This miRNA has previously been shown to be associated with cancer, but not found in normal tissue, and may account for its high expression in transformed and tumor cell lines. We reasoned that inclusion of the mir-19aT sequence would prevent transgene expression in 293T producer cells.
- the vector In order not to decrease vector titer, we constructed the vector so that the expression cassette, including the 19aT sequence, would be in antisense. In this configuration, the vector genome can be transcribed, and because the 19aT sequence is in antisense orientation the transcript will not be subject to degradation by mir-19a-mediated RNAi.
- pLV.PGKas.GFPas.CTEas.polyAas which resulted in a 10-fold reduction in vector titer compared to the canonical plasmid, which is due to the antisense affect of complementry transcripts produced by the plasmid
- pLV.PGKas.GFPas.19aT.CTEas.polyAas did not produce a vector with lower titer then the pLV.PGK.GFP construct.
- miRNA regulation can be used to prevent expression of a transgene during vector production without negatively affecting vector titer.
- LV.PGK.GFP and LV.PGK.GFP.30aT treated animals showed a widespread pattern of cell expression within the liver, including Kupffer cells, hepatocytes and endothelial cells ( FIG. 5 a ).
- LV.PGK.GFP.142-3pT-treated animals had almost undetectable GFP expression in Kupffer cells, but maintained high levels of GFP in hepatocytes and endothelial cells.
- mice receiving the LV.PGK.GFP vector there was a high frequency of GFP+ splenocytes (>5%), with strong levels of expression, as indicated by FACS analysis ( FIG. 5 c ).
- less than 1% of splenocytes from LV.PGK.GFP.142-3pT-treated animals were GFP+ and only at low intensity.
- Immunohistochemical analysis of these mice revealed the presence of GFP+ cells found almost exclusively in the marginal zone. These cells were not of hematopoietic lineage, as indicated by the negative co-staining for the pan-leukocyte marker CD45 ( FIG.
- transgenic mice were generated using the LV.PGK.GFP.142-3pT vector.
- pan-cellular fluorescence throughout the parenchyma of liver, gut and lung, as well as the stromal architecture of the spleen, thymus, and bone marrow we observed no GFP expression within the hematopoietic lineage cells of these organs ( FIG. 6 b ).
- Hemophilia B mice are completely deficient for clotting FIX, and, as such, they have ⁇ 1% normal clotting activity. In addition, because they do not naturally express FIX, they are highly prone to developing anti-FIX immunity, upon the introduction of FIX antigens.
- hepatocyte-specific FIX expression vectors in order to prevent gene expression in APCs, and avoid the induction of anti-FIX immunity (Brown et al., 2004a; Brown et al., 2004b; Follenzi et al., 2004; Mingozzi et al., 2003).
- the hepatocyte-specific LV.ET.hFIX vector was unable to provide long-term FIX expression in hemophilia B mice following intravenous administration.
- endogenous miRNA regulation provided a better means for preventing vector expression in hematopoietic lineage cells then the use of the hepatocyte-specific albumin promoter. This most likely occurred because post-transcriptional regulation can overcome off-target expression due to positional effects of insertion and/or imperfect reconstitution of a tissue-specific promoter. This phenomenon may be akin to one of the proposed natural functions of miRNA regulation, which is to prevent translation of mRNAs that were transcribed in a previous cellular state or that arise due to leaky transcription (Bartel and Chen, 2004; Farh et al., 2005). As such, incorporating miRNA regulation into a vector can provide an important layer of control over transgene expression, whether used with ubiquitous promoters or in conjunction with tissue-specific transcription elements.
- miRNA-mediated regulation is a robust and highly efficient means for virtually abrogating expression from a strong, constitutively active vector promoter, or even for improving the performance of a tissue-specific promoter.
- miRNAs can provide a powerful way to regulate a transgene, and by utilizing this complex network, we have pioneered a new paradigm in vector design that important implications for therapeutic gene transfer.
- MicroRNA expression detected by oligonucleotide microarrays system establishment and expression profiling in human tissues. Genome Res 14, 2486-2494.
- Helper-dependent adenoviral vectors mediate therapeutic factor VIII expression for several months with minimal accompanying toxicity in a canine model of severe hemophilia A. Blood 103, 804-810.
- MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. Proc Natl Acad Sci USA 101, 11755-11760.
- Promoter trapping reveals significant differences in integration site selection between MLV and HIV vectors in primary hematopoietic cells. Blood 105, 2307-2315.
- siRNAs can function as miRNAs. Genes Dev 17, 438-442.
- MicroRNAs small RNAs with a big role in gene regulation. Nat Rev Genet 5, 522-531.
- MicroRNA-responsive ‘sensor’ transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nat Genet 36, 1079-1083.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20200206285A1 (en) * | 2017-07-26 | 2020-07-02 | Oncorus, Inc. | Oncolytic viral vectors and uses thereof |
US11913012B2 (en) | 2018-08-30 | 2024-02-27 | Tenaya Therapeutics, Inc. | Cardiac cell reprogramming with myocardin and ASCL1 |
Families Citing this family (131)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2603881A1 (en) | 2005-04-04 | 2006-10-12 | The Board Of Regents Of The University Of Texas System | Micro-rna's that regulate muscle cells |
PL2002003T3 (pl) | 2005-05-27 | 2016-06-30 | Ospedale San Raffaele Srl | Wektor genetyczny zawierający mi-RNA |
EP1904110A4 (en) * | 2005-06-03 | 2009-02-11 | Southern Adelaide Health Servi | TARGETING CELLS HAVING A MODIFIED EXPRESSION MICROARN |
MX2009001281A (es) | 2006-08-01 | 2009-04-16 | Univ Texas | Identificacion de un micro-arn que activa la expresion de la cadena pesada de beta-miosina. |
WO2008071959A1 (en) * | 2006-12-12 | 2008-06-19 | Oxford Biomedica (Uk) Limited | Lentiviral vectors comprising micrornas |
EP2125032A4 (en) * | 2007-02-20 | 2011-02-23 | Mayo Foundation | TREATMENT OF CANCER USING VIRAL NUCLEIC ACID |
WO2009018492A2 (en) | 2007-07-31 | 2009-02-05 | The Board Of Regents Of The University Of Texas System | Micro-rnas that control myosin expression and myofiber identity |
CA2694930C (en) | 2007-07-31 | 2019-11-12 | Board Of Regents, The University Of Texas System | A micro-rna family that modulates fibrosis and uses thereof |
EP2219653B1 (en) * | 2007-11-09 | 2016-12-21 | The Board of Regents of The University of Texas System | Micro-rnas of the mir-15 family modulate cardiomyocyte survival and cardiac repair |
EP2265291B1 (en) | 2008-03-17 | 2016-10-19 | The Board of Regents of The University of Texas System | Identification of micro-rnas involved in neuromuscular synapse maintenance and regeneration |
WO2009142602A1 (en) * | 2008-05-19 | 2009-11-26 | Agency For Science, Technology And Research | Nucleic acid molecule and method of targeting gene expression to gliomas |
US9217155B2 (en) | 2008-05-28 | 2015-12-22 | University Of Massachusetts | Isolation of novel AAV'S and uses thereof |
WO2010045659A1 (en) | 2008-10-17 | 2010-04-22 | American Gene Technologies International Inc. | Safe lentiviral vectors for targeted delivery of multiple therapeutic molecules |
US9938540B2 (en) | 2008-11-12 | 2018-04-10 | Ospedale San Raffaele S.R.L. | Gene vector for inducing transgene-specific immune tolerance |
CN101787373B (zh) * | 2009-01-23 | 2013-06-19 | 中国人民解放军第二军医大学东方肝胆外科医院 | 一种携带外源基因在包装细胞中高效生产的重组病毒载体、其构建方法及其用途 |
JP5943608B2 (ja) | 2009-02-04 | 2016-07-05 | ボード・オブ・リージエンツ,ザ・ユニバーシテイ・オブ・テキサス・システム | 心臓障害の治療におけるmir−208およびmir−499の二重ターゲティング |
CN104087662A (zh) | 2009-04-29 | 2014-10-08 | 阿姆斯特丹大学学术医学中心 | 对抗、预防和/或测定心力衰竭或心力衰竭的风险的工具和方法 |
EP2425001A4 (en) | 2009-04-30 | 2012-11-14 | Univ California | ANTI-HIV COMBINATION VECTORS, TARGETING VECTORS AND METHOD OF USE |
HUE049845T2 (hu) | 2009-04-30 | 2020-10-28 | Ospedale San Raffaele Srl | Génvektor |
WO2010138263A2 (en) | 2009-05-28 | 2010-12-02 | University Of Massachusetts | Novel aav 's and uses thereof |
DE102009050308A1 (de) | 2009-10-22 | 2011-05-05 | Ludwig-Maximilians-Universität München | RNA mit einer Kombination aus unmodifizierten und modifizierten Nucleotiden zur Proteinexpression |
WO2011012316A2 (de) * | 2009-07-31 | 2011-02-03 | Ludwig-Maximilians-Universität | Rna mit einer kombination aus unmodifizierten und modifizierten nucleotiden zur proteinexpression |
WO2011020005A1 (en) | 2009-08-14 | 2011-02-17 | Regeneron Pharmaceuticals, Inc. | miRNA-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE |
US9593347B2 (en) | 2010-04-16 | 2017-03-14 | University of Pittsburgh—of the Commonwealth System of Higher Education | Identification of mutations in herpes simplex virus envelope glycoproteins that enable or enhance vector retargeting to novel non-HSV receptors |
CA3049237C (en) | 2010-04-23 | 2024-06-11 | University Of Massachusetts | Cns targeting aav vectors and methods of use thereof |
JP2013533847A (ja) | 2010-04-23 | 2013-08-29 | ユニバーシティ オブ マサチューセッツ | コレステロール関連障害のaavベースの治療 |
US9546369B2 (en) | 2010-04-23 | 2017-01-17 | University Of Massachusetts | Multicistronic expression constructs |
US9476041B2 (en) * | 2010-07-12 | 2016-10-25 | National University Corporation Tottori University | Method for producing novel hipsc by means of siRNA introduction |
CA2805170C (en) | 2010-07-12 | 2021-09-21 | Fatima Bosch Tubert | Gene therapy composition for use in diabetes treatment |
EP2638159B1 (en) * | 2010-11-11 | 2019-04-24 | University of Miami | Compositions, kits and methods for treatment of cardiovascular, immunological, and inflammatory diseases |
JP2014504857A (ja) | 2010-12-15 | 2014-02-27 | ミラゲン セラピューティクス | ロックドヌクレオチドを含むmicroRNA阻害剤 |
WO2012143401A1 (en) | 2011-04-18 | 2012-10-26 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Tissue-specific regulation of transgene expression |
WO2012145624A2 (en) | 2011-04-21 | 2012-10-26 | University Of Massachusetts | Raav-based compositions and methods for treating alpha-1 anti-trypsin deficiencies |
EP3524679B1 (en) * | 2011-08-04 | 2021-10-06 | Yeda Research and Development Co., Ltd. | Micro-rna mir-19 and compositions comprising same for the treatment of medical conditions in which low adrenaline or noradrenaline level is therapeutically beneficial |
MY172718A (en) | 2011-08-05 | 2019-12-11 | Regeneron Pharma | Humanized universal light chain mice |
WO2013043442A1 (en) * | 2011-09-23 | 2013-03-28 | Dharmacon, Inc. | The introduction of modular vector elements during production of a lentivirus |
EP2763703B1 (en) | 2011-10-06 | 2018-02-14 | Miragen Therapeutics, Inc. | Control of whole body energy homeostasis by microrna regulation |
EP2584040A1 (en) | 2011-10-17 | 2013-04-24 | Pharmahungary 2000 Kft. | Compounds for treatment of ischemic injury |
US10501512B2 (en) | 2012-04-02 | 2019-12-10 | Modernatx, Inc. | Modified polynucleotides |
EP3501550A1 (en) | 2012-04-02 | 2019-06-26 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins associated with human disease |
US10501513B2 (en) | 2012-04-02 | 2019-12-10 | Modernatx, Inc. | Modified polynucleotides for the production of oncology-related proteins and peptides |
WO2013152230A1 (en) | 2012-04-04 | 2013-10-10 | The Trustees Of Columbia University In The City Of New York | Smooth muscle specific inhibition for anti-restenotic therapy |
CN103505743A (zh) * | 2012-06-21 | 2014-01-15 | 北京命码生科科技有限公司 | 含功能性microRNA/siRNA的细胞微粒子及其应用 |
US9388408B2 (en) | 2012-06-21 | 2016-07-12 | MiRagen Therapeutics, Inc. | Oligonucleotide-based inhibitors comprising locked nucleic acid motif |
US9163235B2 (en) | 2012-06-21 | 2015-10-20 | MiRagen Therapeutics, Inc. | Inhibitors of the miR-15 family of micro-RNAs |
US9597380B2 (en) | 2012-11-26 | 2017-03-21 | Modernatx, Inc. | Terminally modified RNA |
EP2946014A2 (en) * | 2013-01-17 | 2015-11-25 | Moderna Therapeutics, Inc. | Signal-sensor polynucleotides for the alteration of cellular phenotypes |
DK3889173T3 (da) | 2013-02-15 | 2023-10-09 | Bioverativ Therapeutics Inc | Optimeret faktor viii-gen |
NZ720021A (en) | 2013-10-28 | 2022-08-26 | Univ Pittsburgh Commonwealth Sys Higher Education | Oncolytic hsv vector |
US20150147774A1 (en) * | 2013-11-22 | 2015-05-28 | National University Of Singapore | Expression construct for yeast and a method of using the construct |
WO2015127128A2 (en) | 2014-02-19 | 2015-08-27 | University Of Massachusetts | Recombinant aavs having useful transcytosis properties |
JP6756700B2 (ja) | 2014-03-18 | 2020-09-16 | ユニバーシティ オブ マサチューセッツ | 筋萎縮性側索硬化症を処置するためのrAAVベースの組成物および方法 |
WO2015164786A1 (en) * | 2014-04-25 | 2015-10-29 | University Of Massachusetts | Recombinant aav vectors useful for reducing immunity against transgene products |
WO2016004113A1 (en) | 2014-06-30 | 2016-01-07 | Biogen Ma Inc. | Optimized factor ix gene |
WO2016041588A1 (en) | 2014-09-16 | 2016-03-24 | Universitat Autònoma De Barcelona | Adeno-associated viral vectors for the gene therapy of metabolic diseases |
WO2016054554A1 (en) | 2014-10-03 | 2016-04-07 | University Of Massachusetts | Heterologous targeting peptide grafted aavs |
RU2020140209A (ru) | 2014-10-21 | 2021-01-25 | Юниверсити Оф Массачусетс | Варианты рекомбинантных aav и их применения |
CA2969599A1 (en) * | 2014-12-05 | 2016-06-09 | Universitat Autonoma De Barcelona | Viral vectors for the treatment of diabetes |
JP2018503646A (ja) | 2015-01-20 | 2018-02-08 | ミラゲン セラピューティクス, インコーポレイテッド | miR−92阻害剤およびその使用 |
US10584321B2 (en) | 2015-02-13 | 2020-03-10 | University Of Massachusetts | Compositions and methods for transient delivery of nucleases |
EP3285780A4 (en) | 2015-04-24 | 2018-12-19 | University of Massachusetts | Modified aav constructions and uses thereof |
WO2017007994A1 (en) | 2015-07-08 | 2017-01-12 | American Gene Technologies International Inc. | Hiv pre-immunization and immunotherapy |
US10849920B2 (en) | 2015-10-05 | 2020-12-01 | Modernatx, Inc. | Methods for therapeutic administration of messenger ribonucleic acid drugs |
US10188750B1 (en) | 2015-10-23 | 2019-01-29 | University Of South Florida | Self-replicating cell selective gene delivery compositions, methods, and uses thereof |
CN108883100B (zh) | 2016-01-15 | 2022-11-25 | 美国基因技术国际有限公司 | 用于活化γ-δT细胞的方法和组合物 |
US10137144B2 (en) | 2016-01-15 | 2018-11-27 | American Gene Technologies International Inc. | Methods and compositions for the activation of gamma-delta T-cells |
DK3408382T3 (da) | 2016-01-27 | 2022-06-20 | Oncorus Inc | Onkolytiske virale vektorer og anvendelser deraf |
PT3411478T (pt) | 2016-02-01 | 2022-09-13 | Bioverativ Therapeutics Inc | Genes do fator viii otimizados |
JP7153332B2 (ja) | 2016-02-08 | 2022-10-14 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | Hivワクチン接種および免疫療法 |
EP4094780A3 (en) | 2016-02-12 | 2023-02-08 | University of Massachusetts | Anti-angiogenic mirna therapeutics for inhibiting corneal neovascularization |
EP4036231A1 (en) | 2016-03-09 | 2022-08-03 | American Gene Technologies International Inc. | Combination vectors and methods for treating cancer |
US11976292B2 (en) | 2016-06-08 | 2024-05-07 | American Gene Technologies International Inc. | Non-integrating viral delivery system and methods related thereto |
MX2019000252A (es) | 2016-06-30 | 2019-10-09 | Oncorus Inc | Administracion viral oncolitica pseudotipada de polipeptidos terapeuticos. |
AU2017292582C1 (en) | 2016-07-08 | 2021-11-11 | American Gene Technologies International Inc. | HIV pre-immunization and immunotherapy |
GB201612214D0 (en) | 2016-07-14 | 2016-08-31 | Univ Oxford Innovation Ltd | Method |
WO2018017882A1 (en) | 2016-07-21 | 2018-01-25 | American Gene Technologies International Inc. | Viral vectors for treating parkinson's disease |
AU2017302587A1 (en) * | 2016-07-26 | 2019-02-07 | Senti Biosciences, Inc. | Genetic erasers |
JP2018029521A (ja) * | 2016-08-24 | 2018-03-01 | 国立研究開発法人農業・食品産業技術総合研究機構 | miR398標的核酸配列を含む、遺伝子導入のためのウイルスベクター |
US10457940B2 (en) | 2016-09-22 | 2019-10-29 | University Of Massachusetts | AAV treatment of Huntington's disease |
KR20190075964A (ko) | 2016-10-13 | 2019-07-01 | 유니버시티 오브 매사추세츠 | Aav 캡시드 설계 |
MX2019008105A (es) | 2017-01-06 | 2020-07-22 | Stabilitech Biopharma Ltd | Virus. |
CN107384868A (zh) * | 2017-02-17 | 2017-11-24 | 中国农业科学院上海兽医研究所 | 永生化原代绵羊肺细胞系的构建及其应用 |
WO2018187231A2 (en) | 2017-04-03 | 2018-10-11 | American Gene Technologies International Inc. | Compositions and methods for treating phenylketonuria |
JP2020518648A (ja) * | 2017-05-08 | 2020-06-25 | グリットストーン オンコロジー インコーポレイテッド | アルファウイルス新生抗原ベクター |
WO2018208728A1 (en) | 2017-05-08 | 2018-11-15 | Flagship Pioneering, Inc. | Compositions for facilitating membrane fusion and uses thereof |
AU2018264996A1 (en) | 2017-05-09 | 2019-12-05 | University Of Massachusetts | Methods of treating Amyotrophic Lateral Sclerosis (ALS) |
US11767539B2 (en) | 2017-05-12 | 2023-09-26 | University Of Massachusetts | Viral vector production |
AU2018298884A1 (en) | 2017-07-14 | 2020-02-27 | Immatics Biotechnologies Gmbh | Improved dual specificity polypeptide molecule |
IT201700086403A1 (it) * | 2017-07-27 | 2019-01-27 | Univ Del Piemonte Orientale | Endothelial-specific promoter sequences and uses thereof |
SG11202000764RA (en) | 2017-08-09 | 2020-02-27 | Bioverativ Therapeutics Inc | Nucleic acid molecules and uses thereof |
EP3444347A1 (en) | 2017-08-18 | 2019-02-20 | Universität Heidelberg | Use of anti-crispr polypeptides for specific activation of cas nucleases |
CN111448321A (zh) | 2017-09-22 | 2020-07-24 | 马萨诸塞大学 | Sod1双表达载体及其用途 |
JP2021509253A (ja) * | 2017-10-10 | 2021-03-25 | ナントバイオ,インコーポレイテッド | ウイルス生成ペイロードに対して低い毒性を有する改変ec7細胞 |
SI3724336T1 (sl) * | 2017-12-15 | 2022-05-31 | Medizinische Hochschule Hannover | Izboljšana spojina za zdravljenje srčnega popuščanja |
WO2019133847A1 (en) | 2017-12-29 | 2019-07-04 | Oncorus, Inc. | Oncolytic viral delivery of therapeutic polypeptides |
WO2019136309A1 (en) | 2018-01-04 | 2019-07-11 | Iconic Therapeutics, Inc. | Anti-tissue factor antibodies, antibody-drug conjugates, and related methods |
BR112020015228A2 (pt) | 2018-02-01 | 2020-12-29 | Bioverativ Therapeutics Inc. | Uso de vetores lentivirais que expressam fator viii |
WO2019222403A2 (en) | 2018-05-15 | 2019-11-21 | Flagship Pioneering Innovations V, Inc. | Fusosome compositions and uses thereof |
KR20210043574A (ko) | 2018-07-09 | 2021-04-21 | 플래그쉽 파이어니어링 이노베이션스 브이, 인크. | 푸소좀 조성물 및 이의 용도 |
MX2021001599A (es) | 2018-08-09 | 2021-07-02 | Bioverativ Therapeutics Inc | Moleculas de acido nucleico y sus usos para la terapia genica no viral. |
CN112823209A (zh) | 2018-10-11 | 2021-05-18 | 瑞士苏黎世联邦理工大学 | 使用编码高度紧凑的多输入逻辑门的核酸载体治疗疾病的方法 |
US11352646B2 (en) | 2018-11-05 | 2022-06-07 | American Gene Technologies International Inc. | Vector system for expressing regulatory RNA |
JP2022507454A (ja) | 2018-11-14 | 2022-01-18 | フラッグシップ パイオニアリング イノベーションズ ブイ, インコーポレイテッド | Cns送達のためのフソソーム組成物 |
WO2020102485A1 (en) | 2018-11-14 | 2020-05-22 | Flagship Pioneering Innovations V, Inc. | Fusosome compositions for hematopoietic stem cell delivery |
JP2022507453A (ja) | 2018-11-14 | 2022-01-18 | フラッグシップ パイオニアリング イノベーションズ ブイ, インコーポレイテッド | T細胞送達のためのフソソーム組成物 |
US11421273B2 (en) * | 2018-12-03 | 2022-08-23 | California Institute Of Technology | In situ readout of DNA barcodes |
WO2020118069A2 (en) | 2018-12-06 | 2020-06-11 | Bioverativ Therapeutics Inc. | Use of lentiviral vectors expressing factor ix |
EP3908326A4 (en) * | 2018-12-21 | 2022-10-26 | The Trustees of The University of Pennsylvania | COMPOSITIONS FOR DRG-SPECIFIC REDUCTION OF TRANSGENE EXPRESSION |
CA3124524A1 (en) * | 2019-01-04 | 2020-07-09 | Oncorus, Inc. | Encapsulated rna polynucleotides and methods of use |
KR102248420B1 (ko) * | 2019-03-15 | 2021-05-06 | 주식회사 제너로스 | miR-142-3p의 표적 서열을 포함하는 재조합 벡터 |
CN114026236A (zh) * | 2019-04-24 | 2022-02-08 | 宾夕法尼亚州大学信托人 | 可用于治疗雷特综合征的组合物 |
WO2020243719A1 (en) | 2019-05-30 | 2020-12-03 | Gritstone Oncology, Inc. | Modified adenoviruses |
US20230270885A2 (en) | 2019-09-19 | 2023-08-31 | Genethon | Gene therapy expression system alleviating cardiac toxicity of fkrp |
CA3152600A1 (en) | 2019-09-30 | 2021-04-08 | Andrew KROETSCH | Lentiviral vector formulations |
KR102461417B1 (ko) * | 2019-11-11 | 2022-11-01 | 주식회사 온코크로스 | 아데노바이러스 벡터를 포함하는 전립선암 진단용 조성물 |
US20230304034A1 (en) * | 2020-05-12 | 2023-09-28 | The Trustees Of The University Of Pennsylvania | Compositions for drg-specific reduction of transgene expression |
CN115996758A (zh) | 2020-06-19 | 2023-04-21 | 吉尼松公司 | 使sgcg在肌肉和心脏中充分表达的基因治疗表达*** |
BR112022026127A2 (pt) | 2020-06-24 | 2023-01-17 | Bioverativ Therapeutics Inc | Métodos para remoção de fator viii livre de preparações de vetores lentivirais modificados para expressar referida proteína |
US20230277686A1 (en) | 2020-07-10 | 2023-09-07 | Genethon | A novel muscle-specific promoter |
AU2021320896A1 (en) | 2020-08-06 | 2023-03-23 | Gritstone Bio, Inc. | Multiepitope vaccine cassettes |
EP4199971A1 (en) | 2020-08-23 | 2023-06-28 | Bioverativ Therapeutics Inc. | Modified baculovirus system for improved production of closed-ended dna (cedna) |
KR102626543B1 (ko) * | 2020-09-14 | 2024-01-23 | 주식회사 제너로스 | AIMP2-DX2 및 miR-142의 표적 핵산을 포함하는 재조합 벡터 |
KR20220035693A (ko) * | 2020-09-14 | 2022-03-22 | 주식회사 제너로스 | 표적화된 유전자 전달을 위한 아데노-부속 바이러스 벡터 |
JP7121086B2 (ja) * | 2020-09-25 | 2022-08-17 | ウニベルシダッド アウトノマ デ バルセロナ | 脂肪組織に形質導入するのに有用なアデノ随伴ウイルスベクター |
KR20230110503A (ko) * | 2020-09-30 | 2023-07-24 | 주식회사 제너로스 | AIMP2-DX2 및 선택적으로 miR-142에 대한 표적 서열 및 이의 조성물을 이용한 노인성 황반 질환의 치료방법 |
JP2024518378A (ja) | 2021-05-05 | 2024-05-01 | イマティクス バイオテクノロジーズ ゲーエムベーハー | Prameに特異的に結合する抗原結合タンパク質 |
AU2022332276A1 (en) | 2021-08-23 | 2024-04-04 | Bioverativ Therapeutics Inc. | Optimized factor viii genes |
WO2023028440A2 (en) | 2021-08-23 | 2023-03-02 | Bioverativ Therapeutics Inc. | Baculovirus expression system |
WO2023056331A1 (en) | 2021-09-30 | 2023-04-06 | Bioverativ Therapeutics Inc. | Nucleic acids encoding factor viii polypeptides with reduced immunogenicity |
WO2023142040A1 (en) * | 2022-01-29 | 2023-08-03 | Virogin Biotech Canada Ltd. | Transcriptional and translational dual regulated oncolytic herpes simplex virus vectors |
WO2023218430A1 (en) * | 2022-05-13 | 2023-11-16 | Generoath Co., Ltd. | Methods of treating retinal degenerative diseases using aimp2-dx2 and optionally a target sequence for mir‑142 and compositions thereof |
Citations (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS56169586A (en) | 1981-05-02 | 1981-12-26 | Tokyo Tanabe Co Ltd | Novel beta-galactosidase |
WO1998005635A1 (en) | 1996-08-07 | 1998-02-12 | Darwin Discovery Limited | Hydroxamic and carboxylic acid derivatives having mmp and tnf inhibitory activity |
WO1998007859A2 (en) | 1996-08-23 | 1998-02-26 | Genetics Institute, Inc. | Secreted proteins and polynucleotides encoding them |
WO1998009985A2 (en) | 1996-09-03 | 1998-03-12 | Yeda Research And Development Co. Ltd. | Anti-inflammatory peptides and uses thereof |
WO1999043817A1 (en) | 1998-02-25 | 1999-09-02 | The Dow Chemical Company | High affinity humanized anti-cea monoclonal antibodies |
WO1999043816A1 (en) | 1998-02-25 | 1999-09-02 | The Dow Chemical Company | High affinity humanized anti-tag-72 monoclonal antibodies |
WO2003006465A1 (en) | 2001-07-13 | 2003-01-23 | Cv Therapeutics, Inc. | Partial and full agonist of a adenosine receptors |
WO2003020931A2 (en) | 2001-09-01 | 2003-03-13 | Galapagos Genomics N.V. | Sirna knockout assay method and constructs |
WO2003064665A2 (en) | 2002-02-01 | 2003-08-07 | Oxford Biomedica (Uk) Limited | Viral vector |
WO2003065995A2 (en) | 2002-02-07 | 2003-08-14 | Supergen, Inc. | Method for treating diseases associated with abnormal kinase activity |
WO2004058749A1 (en) | 2002-12-18 | 2004-07-15 | Vertex Pharmaceuticals Incorporated | Benzisoxazole derivatives useful as inhibitors of protein kinases |
WO2004065549A2 (en) | 2003-01-17 | 2004-08-05 | University Of Florida | Small interference rna gene therapy |
WO2004066183A2 (en) | 2003-01-22 | 2004-08-05 | European Molecular Biology Laboratory | Microrna |
WO2005013901A2 (en) | 2003-07-31 | 2005-02-17 | Isis Pharmaceuticals, Inc. | Oligomeric compounds and compositions for use in modulation of small non-coding rnas |
WO2005023986A2 (en) | 2003-09-04 | 2005-03-17 | Isis Pharmaceuticals, Inc. | Microrna as ligands and target molecules |
US20050059005A1 (en) | 2001-09-28 | 2005-03-17 | Thomas Tuschl | Microrna molecules |
US20050075492A1 (en) | 2003-08-07 | 2005-04-07 | Whitehead Institute For Biomedical Research | Methods and products for expression of micro RNAs |
US20050266552A1 (en) * | 2003-12-05 | 2005-12-01 | Doench John G | Reagents and methods for identification of RNAi pathway genes and chemical modulators of RNAi |
US20060058266A1 (en) * | 2004-08-10 | 2006-03-16 | Muthiah Manoharan | Chemically modified oligonucleotides |
US20060200869A1 (en) | 2003-04-24 | 2006-09-07 | Luigi Naldini | Lentiviral vectors carrying synthetic bi-directional promoters and uses thereof |
US20060265771A1 (en) | 2005-05-17 | 2006-11-23 | Lewis David L | Monitoring microrna expression and function |
US20070054872A1 (en) | 2005-08-24 | 2007-03-08 | Mirus Bio Corporation | Regulatable or conditional expression systems |
US20090004668A1 (en) * | 2007-06-22 | 2009-01-01 | The Board Of Trustees Of The Leland Stanford Junior University | Pre-miRNA loop-modulated target regulation |
US9556438B2 (en) * | 2005-05-27 | 2017-01-31 | Fondazione Centro San Raffaele Del Monte Tabor | Gene vector |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2705361B1 (fr) | 1993-05-18 | 1995-08-04 | Centre Nat Rech Scient | Vecteurs viraux et utilisation en thérapie génique. |
FR2705686B1 (fr) | 1993-05-28 | 1995-08-18 | Transgene Sa | Nouveaux adénovirus défectifs et lignées de complémentation correspondantes. |
EP0667912B1 (fr) | 1993-07-13 | 2008-07-02 | Centelion | Vecteurs adenoviraux defectifs et utilisation en therapie genique |
WO1995003400A1 (en) | 1993-07-23 | 1995-02-02 | Johns Hopkins University School Of Medicine | Recombinationally targeted cloning in yeast artificial chromosomes |
US7252989B1 (en) | 1994-04-04 | 2007-08-07 | Board Of Regents, The University Of Texas System | Adenovirus supervector system |
FR2724945B1 (fr) | 1994-09-27 | 1996-12-27 | Centre Nat Rech Scient | Vecteurs viraux et utilisation en therapie genique |
IL116816A (en) | 1995-01-20 | 2003-05-29 | Rhone Poulenc Rorer Sa | Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof |
FR2730504B1 (fr) | 1995-02-13 | 1997-03-28 | Rhone Poulenc Rorer Sa | Procede de preparation de genomes d'adenovirus recombinants |
US7223593B2 (en) * | 2000-01-21 | 2007-05-29 | Biovex Limited | Herpes virus strains for gene therapy |
US20060056344A1 (en) * | 2004-09-10 | 2006-03-16 | Interdigital Technology Corporation | Seamless channel change in a wireless local area network |
-
2006
- 2006-05-26 PL PL06795291T patent/PL2002003T3/pl unknown
- 2006-05-26 JP JP2008512952A patent/JP5956709B2/ja active Active
- 2006-05-26 CA CA2609142A patent/CA2609142C/en active Active
- 2006-05-26 US US11/921,140 patent/US10000757B2/en active Active
- 2006-05-26 EP EP06795291.1A patent/EP2002003B1/en active Active
- 2006-05-26 WO PCT/IB2006/002266 patent/WO2007000668A2/en active Application Filing
- 2006-05-26 DK DK06795291.1T patent/DK2002003T3/en active
- 2006-05-26 EP EP15191665.7A patent/EP3002330A1/en not_active Withdrawn
- 2006-05-26 ES ES06795291.1T patent/ES2564823T3/es active Active
- 2006-05-26 CN CN200680027591.5A patent/CN101287834B/zh active Active
- 2006-05-26 SI SI200632032A patent/SI2002003T1/sl unknown
- 2006-05-26 HU HUE06795291A patent/HUE027013T2/en unknown
- 2006-05-26 KR KR1020137025874A patent/KR20130114758A/ko not_active Application Discontinuation
- 2006-05-26 SG SG201003686-1A patent/SG162726A1/en unknown
-
2007
- 2007-11-27 IL IL187679A patent/IL187679A/en active IP Right Grant
- 2007-12-27 KR KR1020077030594A patent/KR101373548B1/ko active IP Right Grant
-
2013
- 2013-05-08 JP JP2013098229A patent/JP2013226139A/ja not_active Withdrawn
-
2014
- 2014-07-15 US US14/332,222 patent/US9556438B2/en active Active
-
2018
- 2018-06-10 US US16/004,394 patent/US11753643B2/en active Active
-
2023
- 2023-03-28 US US18/191,611 patent/US20240218370A1/en active Pending
Patent Citations (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS56169586A (en) | 1981-05-02 | 1981-12-26 | Tokyo Tanabe Co Ltd | Novel beta-galactosidase |
WO1998005635A1 (en) | 1996-08-07 | 1998-02-12 | Darwin Discovery Limited | Hydroxamic and carboxylic acid derivatives having mmp and tnf inhibitory activity |
WO1998007859A2 (en) | 1996-08-23 | 1998-02-26 | Genetics Institute, Inc. | Secreted proteins and polynucleotides encoding them |
WO1998009985A2 (en) | 1996-09-03 | 1998-03-12 | Yeda Research And Development Co. Ltd. | Anti-inflammatory peptides and uses thereof |
WO1999043817A1 (en) | 1998-02-25 | 1999-09-02 | The Dow Chemical Company | High affinity humanized anti-cea monoclonal antibodies |
WO1999043816A1 (en) | 1998-02-25 | 1999-09-02 | The Dow Chemical Company | High affinity humanized anti-tag-72 monoclonal antibodies |
WO2003006465A1 (en) | 2001-07-13 | 2003-01-23 | Cv Therapeutics, Inc. | Partial and full agonist of a adenosine receptors |
WO2003020931A2 (en) | 2001-09-01 | 2003-03-13 | Galapagos Genomics N.V. | Sirna knockout assay method and constructs |
US20050059005A1 (en) | 2001-09-28 | 2005-03-17 | Thomas Tuschl | Microrna molecules |
WO2003064665A2 (en) | 2002-02-01 | 2003-08-07 | Oxford Biomedica (Uk) Limited | Viral vector |
WO2003065995A2 (en) | 2002-02-07 | 2003-08-14 | Supergen, Inc. | Method for treating diseases associated with abnormal kinase activity |
WO2004058749A1 (en) | 2002-12-18 | 2004-07-15 | Vertex Pharmaceuticals Incorporated | Benzisoxazole derivatives useful as inhibitors of protein kinases |
AU2004206232A1 (en) | 2003-01-17 | 2004-08-05 | University Of Florida Research Foundation Inc. | Small interference RNA gene therapy |
WO2004065549A2 (en) | 2003-01-17 | 2004-08-05 | University Of Florida | Small interference rna gene therapy |
WO2004066183A2 (en) | 2003-01-22 | 2004-08-05 | European Molecular Biology Laboratory | Microrna |
EP1616012B1 (en) | 2003-04-24 | 2007-12-19 | Fondazione Centro San Raffaele Del Monte Tabor | Synthetic bi-directional promoters and uses thereof |
US20060200869A1 (en) | 2003-04-24 | 2006-09-07 | Luigi Naldini | Lentiviral vectors carrying synthetic bi-directional promoters and uses thereof |
WO2005013901A2 (en) | 2003-07-31 | 2005-02-17 | Isis Pharmaceuticals, Inc. | Oligomeric compounds and compositions for use in modulation of small non-coding rnas |
US20050075492A1 (en) | 2003-08-07 | 2005-04-07 | Whitehead Institute For Biomedical Research | Methods and products for expression of micro RNAs |
WO2005023986A2 (en) | 2003-09-04 | 2005-03-17 | Isis Pharmaceuticals, Inc. | Microrna as ligands and target molecules |
US20050266552A1 (en) * | 2003-12-05 | 2005-12-01 | Doench John G | Reagents and methods for identification of RNAi pathway genes and chemical modulators of RNAi |
US20060058266A1 (en) * | 2004-08-10 | 2006-03-16 | Muthiah Manoharan | Chemically modified oligonucleotides |
US20060265771A1 (en) | 2005-05-17 | 2006-11-23 | Lewis David L | Monitoring microrna expression and function |
US9556438B2 (en) * | 2005-05-27 | 2017-01-31 | Fondazione Centro San Raffaele Del Monte Tabor | Gene vector |
US20070054872A1 (en) | 2005-08-24 | 2007-03-08 | Mirus Bio Corporation | Regulatable or conditional expression systems |
US20090004668A1 (en) * | 2007-06-22 | 2009-01-01 | The Board Of Trustees Of The Leland Stanford Junior University | Pre-miRNA loop-modulated target regulation |
Non-Patent Citations (106)
Title |
---|
Annoni, A et al. (2013) EMBO Mol. Med. 5: 1684-1697. |
Annoni, A. et al., EMBO Mol Med (2013) 5, 1684-1697. |
Arts, G., et al., Adenoviral Vectors Expressing siRNAs for Discovery and Validation of Gene Function, Genome Research 2003, vol. 13, pp. 2325-2332. |
Baek D et al., The impact of microRNAs on protein output, Nature, 2008, Sep. 4, 2008, vol. 455, No. 7209, p. 64-71. |
Barad, O., Meiri, E., Avniel, A., Aharonov, R., Barzilai, A., Bentwich, I., Einav, U., Gilad, S., Hurban, P., Karov, Y., et al. (2004). MicroRNA expression detected by oligonucleotide microarrays: system establishment and expression profiling in human tissues. Genome Res 14, 2486-2494. |
Barnes, D., et al., Harnessing Endogenous miRNAs to Control Virus Tissue Tropism as a Strategy for Developing Attenuated Virus Vaccines, Cell Host & Microbe, Sep. 11, 2008, vol. 4, pp. 239-248. |
Bartel, D. P., and Chen, C. Z. (2004). Micromanagers of gene expression: the potentially widespread influence of metazoan microRNAs. Nat Rev Genet 5, 396-400. |
Baskerville, S., and Bartel, D. P. (2005). Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes. Rna 11, 241-247. |
Brennecke J, Stark A, Russell RB, Cohen SM, Principles of microRNA-target recognition, PLoS Biol., Mar. 2005, vol. 3, No. 3, e85. |
Brennecke, Julius, et al. "bantam Encodes a Developmentally Regulated microRNA that Controls Cell Proliferation and Regulates the Proapoptotic Gene hid in Drosophila," Cell (2003) vol. 113, pp. 25-36. |
Brown BD, Gentner B, Cantore A, Colleoni S, Amendola M, Zingale A, Baccarini A, Lazzari G, Galli C, Naldini L. Endogenous microRNA can be broadly exploited to regulate transgene expression according to tissue, lineage and differentiation state. Nat Biotechnol. Dec. 2007;25(12):1457-67. Epub Nov. 16, 2007. |
Brown, B. D., and Lillicrap, D. (2002). Dangerous liaisons: the role of "danger" signals in the immune response to gene therapy. Blood 100, 1133-1140. |
Brown, B. D., Shi, C. X., Powell, S., Hurlbut, D., Graham, F. L., and Lillicrap, D. (2004a). Helper-dependent adenoviral vectors mediate therapeutic factor Viii expression for several months with minimal accompanying toxicity in a canine model of severe hemophilia A. Blood 103, 804-810. |
Brown, B. D., Shi, C. X., Rawle, F. E., Tinlin, S., McKinven, A., Hough, C., Graham, F. L., and Lillicrap, D. (2004b). Factors influencing therapeutic efficacy and the host immune response to helper-dependent adenoviral gene therapy in hemophilia A mice. J Thromb Haemost 2, 111-118. |
Brown, B.D., et al., A microRNA-regulated lentiviral vector mediates stable correction of hemophilia B mice, Blood, Dec. 15, 2007, vol. 110, No. 13, pp. 4144-4152. |
Brown, B.D., et al., Endogenous microRNA can be broadly exploited to regulate transgene expression according to tissue, lineage and differentiation state. Nat Biotechnol. Dec. 2007; 25(12):1457-67. Epub Nov. 16, 2007. |
Brown, B.D., et al., Exploiting and antagonizing microRNA regulation for therapeutic and experimental applications, Nature Reviews Genetics, Aug. 2009, vol. 10, pp. 578-584. |
Brown, Brian D., et al., "Endogenous microRNA regulaion suppresses transgene expression in hematopoietic lineages and enables stable gene transfer," Nature Medicine (2006) vol. 12:5, pp. 585-591. |
Calin G.A. et al., Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia, Proc Natl Acad Sci (2002) 99:15524-15529. |
Calin, G. A., Liu, C. G., Sevignani, C., Ferracin, M., Felli, N., Dumitru, C. D., Shimizu, M., Cimmino, A., Zupo, S., Dono, M., et al. (2004a). MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. Proc Natl Acad Sci U S A 101, 11755-11760. |
Calin, G. A., Sevignani, C., Dumitru, C. D., Hyslop, T., Noch, E., Yendamuri, S., Shimizu, M., Rattan, S., Bullrich, F., Negrini, M., and Croce, C. M. (2004b). Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci U S A 101, 2999-3004. |
Cawood, R.. et al., Use of Tissue-Specific MicroRNA to Control Pathology of Wild-Type Adenovirus without Attenuation of Its Ability to Kill Cancer Cells, PLoS Pathogens May 2009, vol. 5, No. 5, e1000440. |
Chen et al., "MicroRNAs as regulators of mammalian hematopoiesis," Semin Immunol., vol. 17(2), p. 155-65 (Apr. 2005). |
Chen et al., "MicroRNAs modulate hematopoietic lineage differentiation," Science, vol. 303(5654), p. 83-6 (Jan. 2004). |
Cheng A M et al., Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis, Nucleic Acids Research, Mar. 2005 vol. 33, No. 4, p. 1290-1297. |
Chiriaco, M. et al. (2014) Mol. Ther. 22: 1472-1483. |
Chiriaco, M. et al., Mol Ther, Aug. 2014;22(8):1472-83. |
De Geest, B. R., Van Linthout, S. A., and Collen, D. (2003). Humoral immune response in mice against a circulating antigen induced by adenoviral transfer is strictly dependent on expression in antigen-presenting cells. Blood 101, 2551-2556. |
De Palma, M., Montini, E., de Sio, F. R., Benedicenti, F., Gentile, A., Medico, E., and Naldini, L. (2005). Promoter trapping reveals significant differences in integration site selection between MLV and HIV vectors in primary hematopoietic cells. Blood 105, 2307-2315. |
Dobrzynski E, Mingozzi F, Liu YL, bendo E, Cao O, Wang L, Herzog RW, Induction of antigen-specific CD4+ T-cell anergy and eletion by in vivo viral gene transfer, Blood, Aug. 15, vol. 104, No. 4, p. 969-977. Epub Apr. 22, 2004. |
Doench et al. (Genes & Development, vol. 18, No. 5, pp. 504-511 (2004). * |
Doench, J. G., Petersen, C. P., and Sharp, P. A. (2003). siRNAs can function as miRNAs. Genes Dev 17, 438-442. |
Escobar et al., Sci Transl Med 6, 217ra3 (2014). |
Escobar, G. et al. (2014) OncoImmunology e28696. |
Escobar, G. et al. (2014) Sci. Transl. Med. 6: 217ra3. |
Extended European Search Report dated Jan. 18, 2016, issued in EP Application No. 15191665.7. |
Farh, K. K., Grimson, A., Jan, C., Lewis, B. P., Johnston, W. K., Lim, L. P., Burge, C. B., and Bartel, D. P. (2005). The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Science 310, 1817-1821. |
Fischer A et al., Gene therapy of inherited diseases, Lancet, Jun. 14, 2008, vol. 371, p. 2044-2047. |
Follenzi, A., Battaglia, M., Lombardo, A., Annoni, A., Roncarolo, M. G., and Naldini, L. (2004). Targeting lentiviral vector expression to hepatocytes limits transgene-specific immune response and establishes long-term expression of human antihemophilic factor IX in mice. Blood 103, 3700-3709. |
Follenzi, A., Sabatino, G., Lombardo, A., Boccaccio, C., and Naldini, L. (2002). Efficient gene delivery and targeted expression to hepatocytes in vivo by improved lentiviral vectors. Hum Gene Ther 13, 243-260. |
Geisler et al.,World J Exp Med, May 20, 2016; 6(2): 37-54. |
Gentner, B. et al. (2010) Sci. Transl. Med. 2: 58ra84. |
Giraldez A J et al., MicroRNAs Regulate Brain Morphogenesis in Zebrafish, Science May 6, 2005, vol. 308 pp. 833-838. |
Gong, H., et al., "The Role of Small RNAs in Human Diseases: Potential Troublemaker and Therapeutics," Medic. Res. Rev. (2005) vol. 25, pp. 361-381. |
Gottwein E et al., Viral and cellular microRNAs as determinants of viral, Cell Host Microbe Jun. 12, 2008, vol. 3, No. 6, p. 375-387. |
Griffiths-Jones, S., Grocock, R. J., van Dongen, S., Bateman, A., and Enright, A. J. (2006). miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res 34, D140-144. |
Grimm, D,. et al., Therapeutic application of RNAi: is mRNA targeting finally ready for prime time?, The Journal of Clinical Investigation, Dec. 2007, vol. 117, No. 12, pp. 3633-3641. |
He, L., et al., MicroRNAs: Small RNAs with a big role in gene regulation, Nature Reviews Genetics, Jul. 2004, vol. 5, pp. 522-531. |
Houbaviy HB et al., Embryonic Cell-Specific MicroRNAs, Developmental Cell, 2003, 5, 351-358. |
Huber B E et al., Retroviral-mediated gene therapy for the treatment of hepatocellular carcinoma: An innovative approach for cancer therapy, Proc. Natl. Acad. Sci. USA, 1991, vol. 88, p. 8039-8043. |
Iorio, M. V., Ferracin, M., Liu, C. G., Veronese, A., Spizzo, R., Sabbioni, S., Magri, E., Pedriali, M., Fabbri, M., Campiglio, M., et al. (2005). MicroRNA gene expression deregulation in human breast cancer. Cancer Res 65, 7065-7070. |
Japanese Office Action dated Nov. 26, 2015 issued in JP Application No. 2008-512952. |
Johnson, Steven M., et al., "The time of appearance of C. elegans let-7 micorRNA is transcriptionally controlled utilizing a temporal regulatory element in its promoter," Developmental Biology (2003) vol. 259, pp. 364-379. |
Kasashima et al., "Altered expression profiles of microRNAs during TPA-induced differentiation of HL-60 cells," Biochem Biophys Res Commun., vol. 322(2), p. 403-10 (Sep. 2004). |
Kay M, State-of-the-art gene-based therapies: the road ahead, Nat Rev Genet May 2011, vol. 12, p. 316-328. |
Kay, M.A., MicroRNAs outwit immune limitations in gene therapy, Blood, Dec. 15, 2007, vol. 110, No. 13, pp. 4136-4137. |
Kay, M.A., State-of-the-art gene-base therapies: the road ahead, Nature Reviews Genetics, Apr. 6, 2011 (Advance Online publication). |
Kelly, E.J., et al., Engineering microRNA responsiveness to decrease virus pathogenicity, Nature Medicine, Nov. 2008, vol. 14, No. 11, pp. 1278-1283. |
Kelly, E.J., et al., MicroRNAs and the Regulation of Vector Tropism, Molecular Therapy, 2009, vol. 17, No. 3, pp. 409-416. |
Kootstra, N.A., et al., Efficient Production of Human FVIII in Hemophilic Mice Using Lentiviral Vectors, Molecular Therapy, 2003, vol. 7, No. 5, pp. 623-631. |
Krichevsky et al, A microRNA array reveals extensive regulation of microRNAs during brain development, RNA (2003), 9:1274-1281. |
Lagos-Quintana et al., "Identification of tissue-specific microRNAs from mouse," Curr. Biol. vol. 12(9), p. 735-9 (Apr. 30, 2002). |
Lai, E.C., et al. "Pervasive regulation of Drosophila Notch target genes by GY-box-, Brd-box-, and K-box-class microRNAs," Genes Dev. (2005) vol. 19 pp. 1067-1080. |
LoDuca P et al., Hepatic Gene Transfer as a Means of Tolerance Induction to Transgene Products, Curr Gene Ther, Apr. 2009, vol. 9, No. 2, p. 104-114. |
Mansfield JH et al., MicroRNA-responsive "sensor" transgenes uncover Hox-like and other develomentally regulated patterns of vertebrate microRNA expression, Nature Genetics, 2004, 36(10):1079-83 Epub, erratum in Nat Genet (2004) 36(11):1238. |
Mansfield, J. H., et al., "Micro-RNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patters of vertebrate microRNA expression," Nat. Genetics (2004) vol. 36, pp. 1079-1083. |
Mansfield, J. H., et al., "Micro-RNA-responsive ‘sensor’ transgenes uncover Hox-like and other developmentally regulated patters of vertebrate microRNA expression," Nat. Genetics (2004) vol. 36, pp. 1079-1083. |
Mansfield, J. H., Harfe, B. D., Nissen, R., Obenauer, J., Srineel, J., Chaudhuri, A., Farzan-Kashani, R., Zuker, M., Pasquinelli, A. E., Ruvkun, G., et al. (2004). MicroRNA-responsive ‘sensor’ transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nat Genet 36, 1079-1083. Epub, erratum in Nat Genet (2004) 36(11):1238. |
Mansfield, J. H., Harfe, B. D., Nissen, R., Obenauer, J., Srineel, J., Chaudhuri, A., Farzan-Kashani, R., Zuker, M., Pasquinelli, A. E., Ruvkun, G., et al. (2004). MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nat Genet 36, 1079-1083. Epub, erratum in Nat Genet (2004) 36(11):1238. |
Mansfield, Jennifer H., et al., "MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression," Nature Genetics (2004) vol. 36:10, pp. 1079-1083. |
Mansfield, Jennifer H., et al., "MicroRNA-responsive ‘sensor’ transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression," Nature Genetics (2004) vol. 36:10, pp. 1079-1083. |
Marquez, R.T., et al., Advances in MicroRNAs: Implications for Gene Therapists, Human Gene Therapy, Jan. 2008, vol. 19, p. 27-37. |
Mátrai, J. et al. (2011) Hepatology 53: 1696-1707. |
Mátrai, J., et al., Recent Advances in Lentiviral Vector Development and Applications, Molecular Therapy, Mar. 2010, vol. 18, No. 3, pp. 477-490. |
McManus, et al., "Gene silencing in mammals by small interfering RNAs", Nature Reviews, Oct 2002, vol. 3, p. 737-747. |
Metzler M et al, High Expression of Precursor MicroRNA-155/BIC RNA in Children with Burkitt Lymphoma, Genes Chromosomes Cancer (2004) 39:167-169. |
Michael et al., "Reduced accumulation of specific microRNAs in colorectal neoplasia," Mol. Cancer Res., vol. 1(12), p. 882-91 (Oct. 2003). |
Mingozzi, F., Liu, Y. L., Dobrzynski, E., Kaufhold, A., Liu, J. H., Wang, Y., Arruda, V. R., High, K. A., and Herzog, R. W. (2003). Induction of immune tolerance to coagulation factor Ix antigen by in vivo hepatic gene transfer. J Clin Invest 111, 1347-1356. |
Mitta B et al.., Advanced modular self-inactivating lentiviral expression vectors for multigene interventions in mammalian cells and in vivo transduction, Nucleic Acids Research, 2002 vol. 30, n0. 21 e113, 1-18. |
Mochizuki et al., "Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymena," Cell, vol. 110(6), p. 689-99 (Sep. 2002). |
Odom, G.L., et al., Viral-mediated gene therapy for the muscular dystrophies: Success, limitations and recent advances, Biochimica et Biophysica Acta, 2007, vol. 1772, pp. 243-262. |
Opalinska et al. (Nature Rev., vol. 1: pp. 503-514 (2002). * |
Perez, J.T., et al., Micro-RNA-mediated species-specific attenuation of influenza A virus, Nature Biotechnology, Jun. 2009, vol. 27, No. 6, pp. 572-578. |
Pfeifer A et al., Transgenesis by lentivral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos, Proc. Natl. Acad. Sci., 2002, vol. 99, No. 4, p. 2140-2145. |
Pierce, G.F,. et al., Gene therapy, bioengineered clotting factors and novel technologies for haemophilia treatment, Journal of Thrombosis and Haemostasis, 2007, vol. 5, pp. 901-906. |
Qasim, W., et al., RISC control for gene therapy, Nature Biotechnology, Jun. 2006, vol. 24, No. 6, pp. 661-662. |
Qiao et al, Gene Therapy (2011) 18, 403-410. |
Reinhart, B. J., Slack, F. J., Basson, M., Pasquinelli, A. E., Bettinger, J. C., Rougvie, A. E., Horvitz, H. R., and Ruvkun, G. (2000). The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 403, 901-906. |
Robbins P D et al., Viral Vectors for Gene Therapy, Pharmacol Ther., 1998, vol. 80, No. 1, p. 35-47. |
Saenz DT, Loewen N, Peretz M, Whitwam T, Barraza R, Howell KG, Holmes JM, Good M, Poeschla EM: Unintegrated lentivirus DNA persistence and accessibility to expressionin nondividing cells: Analysis with class I inegrase mutants, J Virol, 2004, vol. 78, No. 6, p. 2906-2920. |
Sandrin, V., Russell, S. J., and Cosset, F. L. (2003). Targeting retroviral and lentiviral vectors. Curr Top Microbiol Immunol 281, 137-178. |
Selbach M et al., Widespread changes in protein synthesis induced by microRNA, Nature, 2008, vol. 455, p. 58-63. |
Sempere et al., Genome Biology 2004, 5:R13. |
Sempere, L. F., Freemantle, S., Pitha-Rowe, I., Moss, E., Dmitrovsky, E., and Ambros, V. (2004). Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation. Genome Biol 5, R13. |
Smirnova L et al., Regulation of miRNA expression during neural cell specification, Eur J Neurosci, Mar. 2005, vol. 21 pp. 1469-1477. |
Song J et al., Gene Silencing in Androgen-responsive prostate Cancer Cells from Tissue-Specific Prostate-Specific Antigen Promoter, Cancer Research, 2004, vol. 64, p. 7661-7663. |
Stark A et al., Identification of Drosophila MicroRNA Targets, PloS Biol., Oct. 13, 2003, vol. 1, issue 3, p. 397-409. |
Stöven S et al., Caspase-mediated processing of the Drosophila NF-κb factor Relish, Proc Natl Arad Sci USA, Mar. 2003, vol. 100, No. 17, pp. 9779-9784. |
Tenoever BR, RNA viruses and the host microRNA machinery, Nat Rev Microbiol Mar. 2013, vol. 11, p. 169-180. |
Thomas, C. E., Ehrhardt, A., and Kay, M. A. (2003). Progress and problems with the use of viral vectors for gene therapy. Nat Rev Genet 4, 346-358. |
Verma et al. (Annu. Rev. Biochem (2005) 74:71-38). * |
Verma, I. M., and Weitzman, M. D. (2005). Gene Therapy: Twenty-First Century Medicine. Annu Rev Biochem 74, 711-738. |
Xie, J., et al., MicroRNA-regulated, Systemically Delivered rAAV9: A Step Closer to CNS-restricted Transgene Expression, Mar. 2011, vol. 19, No. 3, pp. 526-535. |
Yanez-Munoz RJ, Balaggan KS, MacNeil A, Howe SJ, Schmidt M, Smith AJ, Buch P, MacLaren RE, Anderson PN, Barker SE, Duran Y et al., Effective gene therapy with nonineegrating lentiviral vectors, Nat Med, 2006, nol. 12, No. 3, p. 348-353. |
Zahler M H et al., The application of a lentiviral vector for gene transfer in fetal human hepatocytes, Journal of Gene Medicine, 2000, vol. 2, p. 186-193. |
Zeng, Y., Wagner, E. J., and Cullen, B. R. (2002). Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol Cell 9, 1327-1333. |
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