MX2010010076A - Metodo para desnaturalizar proteina con enzimas. - Google Patents

Metodo para desnaturalizar proteina con enzimas.

Info

Publication number
MX2010010076A
MX2010010076A MX2010010076A MX2010010076A MX2010010076A MX 2010010076 A MX2010010076 A MX 2010010076A MX 2010010076 A MX2010010076 A MX 2010010076A MX 2010010076 A MX2010010076 A MX 2010010076A MX 2010010076 A MX2010010076 A MX 2010010076A
Authority
MX
Mexico
Prior art keywords
protein
transglutaminase
glutaminase
enzymes
quantitative ratio
Prior art date
Application number
MX2010010076A
Other languages
English (en)
Inventor
Noriko Miwa
Hiroyuki Nakagoshi
Fumiyuki Hirose
Nobuhisa Shimba
Mina Nakamura
Eiichiro Suzuki
Keiichi Yokoyama
Hiroaki Sato
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of MX2010010076A publication Critical patent/MX2010010076A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G01N2333/91074—Aminoacyltransferases (general) (2.3.2)
    • G01N2333/9108—Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
    • G01N2333/91085—Transglutaminases; Factor XIIIq (2.3.2.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Dairy Products (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se proveen un método para desnaturalizar una proteína tratando la proteína con proteína glutaminasa y transglutaminasa; un alimento que contiene una proteína habiendo sido desnaturalizada con estas enzimas; una preparación de enzima para desnaturalizar una proteína que comprende estas enzimas; y un método para establecer la relación cuantitativa óptima de proteína glutaminasa a transglutaminasa las cuales se añadirán a una proteína; una proteína es desnaturalizada añadiendo proteína glutaminasa y transglutaminasa a la proteína sustancialmente en la misma oportunidad, o añadiendo proteína glutaminasa a la proteína antes de que la transglutaminasa actúe sobre la proteína, o controlando la relación cuantitativa de proteína glutaminasa a transglutaminasa, por lo cual se trata una proteína, a un nivel definido; la relación cuantitativa óptima de proteína glutaminasa a transglutaminasa, las cuales se añadirán a una proteína, se determina con base en la intensidad de la señal de RMN de la proteína marcada con amonio marcado con isótopos, o simílares.
MX2010010076A 2008-03-14 2009-03-12 Metodo para desnaturalizar proteina con enzimas. MX2010010076A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2008066765 2008-03-14
JP2008294890 2008-11-18
PCT/JP2009/054792 WO2009113628A1 (ja) 2008-03-14 2009-03-12 酵素によるタンパク質の改質方法

Publications (1)

Publication Number Publication Date
MX2010010076A true MX2010010076A (es) 2010-10-20

Family

ID=41065288

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010010076A MX2010010076A (es) 2008-03-14 2009-03-12 Metodo para desnaturalizar proteina con enzimas.

Country Status (10)

Country Link
EP (1) EP2267146A4 (es)
JP (1) JP5616216B2 (es)
KR (1) KR101577601B1 (es)
CN (1) CN102016057A (es)
BR (1) BRPI0910821B1 (es)
CA (1) CA2717340A1 (es)
MX (1) MX2010010076A (es)
MY (1) MY155718A (es)
TW (1) TW200942616A (es)
WO (1) WO2009113628A1 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100846898B1 (ko) 2005-12-30 2008-07-17 엘지전자 주식회사 영상 디스플레이 기기의 스탠드
JP4856665B2 (ja) * 2008-03-14 2012-01-18 味の素株式会社 麺類の製造法
CN101861909B (zh) * 2010-05-05 2012-09-12 长沙理工大学 一种蛋白质谷氨酰胺酶对大米蛋白和米谷蛋白改性的方法
JP5719752B2 (ja) * 2011-10-28 2015-05-20 森永乳業株式会社 発酵乳の製造方法
CN104736135B (zh) * 2012-10-17 2019-07-09 味之素株式会社 化妆品组合物
CN103263688B (zh) * 2013-05-10 2014-07-30 华东师范大学 一种明胶组合物及其制备方法和应用
CN103478701B (zh) * 2013-09-24 2015-04-22 河南翔宇食品有限公司 一种无淀粉香菇素肠及其加工方法
CN104782878A (zh) * 2015-05-06 2015-07-22 东北农业大学 一种酶法改性乳清蛋白可溶性聚合物的制备方法
WO2017170657A1 (ja) 2016-03-30 2017-10-05 味の素株式会社 ヨーグルトの製造方法
CN106434799A (zh) * 2016-09-28 2017-02-22 华南理工大学 一种蛋白质纳米颗粒及其制备方法
CN106858587A (zh) * 2017-01-18 2017-06-20 华南理工大学 大豆分离蛋白‑姜黄素纳米颗粒结合物及其制备方法
WO2021002436A1 (ja) * 2019-07-03 2021-01-07 味の素株式会社 改質したえんどう豆たん白質の製造方法
EP4130286A4 (en) * 2020-04-03 2024-03-20 Amano Enzyme Inc. PROCESS FOR DEAMIDATION OF A PROTEIN
CN115867144A (zh) * 2020-06-08 2023-03-28 天野酶制品株式会社 蛋白质口感改良
WO2022014542A1 (ja) * 2020-07-13 2022-01-20 天野エンザイム株式会社 植物性タンパク質食品の製造方法
US20220053786A1 (en) * 2020-08-19 2022-02-24 Ajinomoto Co., Inc. Methods for producing stirred yogurt
EP4201216A1 (en) * 2020-08-24 2023-06-28 Amano Enzyme Inc. Vegetable milk treated with protein deamidase
CN114304276A (zh) * 2020-09-30 2022-04-12 天野酶制品株式会社 加工植物性奶的制造方法
US20230416718A1 (en) 2020-11-20 2023-12-28 Amano Enzyme Inc. Thermotolerant protein glutaminase
WO2023085315A1 (ja) * 2021-11-09 2023-05-19 天野エンザイム株式会社 植物性タンパク質含有組成物の消化性向上剤
CN114403280B (zh) * 2022-01-30 2023-06-09 杭州佳嘉乐生物技术有限公司 一种具有多种功能的纳米级牡蛎肽粉的制备方法
CN114836403B (zh) * 2022-04-28 2023-08-25 江南大学 一种利用蛋白质谷氨酰胺酶改善糖酶催化性能的方法
WO2024053745A1 (ja) * 2022-09-09 2024-03-14 天野エンザイム株式会社 加工植物性タンパク質含有組成物の製造方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58149645A (ja) 1982-03-01 1983-09-06 Ajinomoto Co Inc ゲル化物の製造法
JPH0665280B2 (ja) 1987-03-04 1994-08-24 味の素株式会社 タンパクゲル化剤及びそれを用いるタンパクのゲル化方法
JPH01300889A (ja) 1988-05-30 1989-12-05 Ajinomoto Co Inc 形質転換体及びこれを用いるＭＴＧａｓｅの製造法
JP3364972B2 (ja) 1992-01-14 2003-01-08 味の素株式会社 魚由来トランスグルタミナーゼ遺伝子
JPH0723737A (ja) 1993-07-06 1995-01-27 Nippon Oil & Fats Co Ltd 液状飲食用組成物およびその製造方法
JP3609648B2 (ja) * 1998-06-04 2005-01-12 天野エンザイム株式会社 新規蛋白質脱アミド酵素、それをコードする遺伝子、その製造法並びにその用途
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DK1106696T3 (da) 1999-12-03 2005-08-01 Amano Enzyme Inc Proteindeamiderende enzym, mikroorganisme producerende dette, gen kodende derfor, fremgangsmåde til fremstilling af enzymet og anvendelse deraf
JP4596207B2 (ja) 2000-05-15 2010-12-08 味の素株式会社 酵素によるタンパク質の同位体標識法
US6762041B2 (en) * 2000-05-15 2004-07-13 Ajinomoto Co., Inc. Method for isotope labeling of protein with enzyme
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MY148140A (en) * 2005-01-13 2013-02-28 Amano Enzyme Inc A processed meat product or a fish paste product and method for producing the same

Also Published As

Publication number Publication date
KR101577601B1 (ko) 2015-12-15
EP2267146A1 (en) 2010-12-29
CA2717340A1 (en) 2009-09-17
MY155718A (en) 2015-11-30
BRPI0910821A2 (pt) 2015-08-18
JP5616216B2 (ja) 2014-10-29
BRPI0910821A8 (pt) 2017-08-08
EP2267146A4 (en) 2011-05-04
KR20100127824A (ko) 2010-12-06
CN102016057A (zh) 2011-04-13
WO2009113628A1 (ja) 2009-09-17
TW200942616A (en) 2009-10-16
JPWO2009113628A1 (ja) 2011-07-21
BRPI0910821B1 (pt) 2018-07-10

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