KR20010073216A - 약 15kDa 및 약 45kDa 살충성 단백질을 코딩하는식물에 최적화된 폴리뉴클레오티드 - Google Patents
약 15kDa 및 약 45kDa 살충성 단백질을 코딩하는식물에 최적화된 폴리뉴클레오티드 Download PDFInfo
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Abstract
Description
계대배양주 | 수탁번호 | 기탁일 |
B.t.PS80JJ1 | NRRL B-18679 | 1990. 7. 17 |
E. coli(NM522)(pMYC2421)(PS80JJ1 14kDa & 45kDa) | NRRL B-21555 | 1996. 3. 28 |
E. coli(NM522)(pMYC2426)(PS80JJ1 14kDa & 45kDa) | NRRL B-21671 | 1997. 3. 26 |
B.t.PS149B1 | NRRL B-21553 | 1996. 4. 8 |
E. coli(NM522)(pMYC2429)(PS149B1 15kDa & 45kDa) | NRRL B-21673 | 1997. 3. 26 |
아미노산 클래스 | 아미노산 예 |
비극성 | Ala, Val, Leu, Ile, Pro, Met, Phe, Trp |
전하를 띠지 않은 극성 | Gly, Ser, Thr, Cys, Tyr, Asn, Gln |
산성 | Asp, Glu |
염기성 | Lys, Arg, His |
Claims (25)
- 서열 2, 서열 5, 및 서열 6으로 구성된 그룹으로부터 선택되는 뉴클레오티드 서열을 갖는 폴리뉴클레오티드.
- 제 1항에 있어서, 상기 뉴클레오티드 서열은 서열 2인 것을 특징으로 하는 폴리뉴클레오티드.
- 제 1항에 있어서, 상기 뉴클레오티드 서열은 서열 5인 것을 특징으로 하는 폴리뉴클레오티드.
- 제 1항에 있어서, 상기 뉴클레오티드 서열은 서열 6인 것을 특징으로 하는 폴리뉴클레오티드.
- 제 1항에 따른 폴리뉴클레오티드를 발현하는 재조합 숙주.
- 제 5항에 있어서, 상기 뉴클레오티드 서열은 서열 2인 것을 특징으로 하는 숙주.
- 제 5항에 있어서, 상기 뉴클레오티드 서열은 서열 5인 것을 특징으로 하는숙주.
- 제 5항에 있어서, 상기 뉴클레오티드 서열은 서열 6인 것을 특징으로 하는 숙주.
- 제 5항에 있어서, 상기 숙주는 식물 세포인 것을 특징으로 하는 숙주.
- 제 5항에 있어서, 상기 숙주는 식물인 것을 특징으로 하는 숙주.
- 제 5항에 있어서, 상기 숙주는 옥수수인 것을 특징으로 하는 숙주.
- 제 5항의 재조합 숙주를 생산하는 방법.
- 제 12항에 있어서, 상기 숙주는 식물 세포인 것을 특징으로 하는 방법.
- 제 12항에 있어서, 상기 숙주는 식물인 것을 특징으로 하는 방법.
- 제 12항에 있어서, 상기 숙주는 옥수수인 것을 특징으로 하는 방법.
- 식물 해충을 구제하기 위한 방법으로서, 상기 방법은 해충을 제 1항에 따른폴리뉴클레오티드에 의해 코딩되는 제 1의 단백질과 접촉시키는 단계를 포함하며, 상기 제 1의 단백질은 상기 폴리뉴클레오티드를 발현하는 재조합 숙주에 의해 생산되는 것을 특징으로 하는 방법.
- 제 16항에 있어서, 상기 숙주는 식물인 것을 특징으로 하는 방법.
- 제 16항에 있어서, 상기 숙주는지아 메이스(Zea mays) 식물인 것을 특징으로 하는 방법.
- 제 18항에 있어서, 상기 뉴클레오티드 서열은 서열 2인 것을 특징으로 하는 방법.
- 제 18항에 있어서, 상기 뉴클레오티드 서열은 서열 5인 것을 특징으로 하는 방법.
- 제 18항에 있어서, 상기 뉴클레오티드 서열은 서열 6인 것을 특징으로 하는 방법.
- 제 19항에 있어서, 상기 방법은 상기 해충을 약 45kDa의 제 2의 단백질과 접촉시키는 단계를 추가로 포함하는 것을 특징으로 하는 방법.
- 제 20항에 있어서, 상기 방법은 상기 해충을 약 45kDa의 제 2의 단백질과 접촉시키는 단계를 추가로 포함하는 것을 특징으로 하는 방법.
- 제 21항에 있어서, 상기 방법은 상기 해충을 약 15kDa의 제 2의 단백질과 접촉시키는 단계를 추가로 포함하는 것을 특징으로 하는 방법.
- 서열 3의 아미노산 서열을 갖는 살충성 단백질.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10535998P | 1998-10-23 | 1998-10-23 | |
US10540898P | 1998-10-23 | 1998-10-23 | |
US60/105,408 | 1998-10-23 | ||
US60/105,359 | 1998-10-23 | ||
PCT/US1999/024647 WO2000024904A1 (en) | 1998-10-23 | 1999-10-21 | PLANT-OPTIMIZED POLYNUCLEOTIDES ENCODING APPROXIMATELY 15 kDa AND APPROXIMATELY 45 kDa PESTICIDAL PROTEINS |
Publications (1)
Publication Number | Publication Date |
---|---|
KR20010073216A true KR20010073216A (ko) | 2001-07-31 |
Family
ID=26802496
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020017004769A KR20010073216A (ko) | 1998-10-23 | 1999-10-21 | 약 15kDa 및 약 45kDa 살충성 단백질을 코딩하는식물에 최적화된 폴리뉴클레오티드 |
Country Status (13)
Country | Link |
---|---|
US (1) | US6340593B1 (ko) |
EP (1) | EP1124967B1 (ko) |
JP (2) | JP4647099B2 (ko) |
KR (1) | KR20010073216A (ko) |
CN (1) | CN1324407A (ko) |
AR (2) | AR020944A1 (ko) |
AT (1) | ATE327333T1 (ko) |
AU (1) | AU768246B2 (ko) |
BR (1) | BR9914746A (ko) |
CA (2) | CA2703161C (ko) |
DE (2) | DE69940962D1 (ko) |
ES (2) | ES2264283T3 (ko) |
WO (1) | WO2000024904A1 (ko) |
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1999
- 1999-10-21 DE DE69940962T patent/DE69940962D1/de not_active Expired - Lifetime
- 1999-10-21 WO PCT/US1999/024647 patent/WO2000024904A1/en not_active Application Discontinuation
- 1999-10-21 ES ES99971037T patent/ES2264283T3/es not_active Expired - Lifetime
- 1999-10-21 BR BR9914746-7A patent/BR9914746A/pt not_active Application Discontinuation
- 1999-10-21 AU AU12181/00A patent/AU768246B2/en not_active Expired
- 1999-10-21 DE DE69931511T patent/DE69931511T2/de not_active Expired - Lifetime
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- 1999-10-21 KR KR1020017004769A patent/KR20010073216A/ko active Search and Examination
- 1999-10-21 AT AT99971037T patent/ATE327333T1/de not_active IP Right Cessation
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Also Published As
Publication number | Publication date |
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DE69940962D1 (de) | 2009-07-16 |
CA2703161A1 (en) | 2000-05-04 |
DE69931511T2 (de) | 2006-09-28 |
AR066201A2 (es) | 2009-08-05 |
CN1324407A (zh) | 2001-11-28 |
JP4912473B2 (ja) | 2012-04-11 |
US6340593B1 (en) | 2002-01-22 |
CA2345905A1 (en) | 2000-05-04 |
BR9914746A (pt) | 2001-10-30 |
JP2010131021A (ja) | 2010-06-17 |
JP4647099B2 (ja) | 2011-03-09 |
JP2002528083A (ja) | 2002-09-03 |
ES2264283T3 (es) | 2006-12-16 |
CA2345905C (en) | 2010-08-03 |
DE69931511D1 (de) | 2006-06-29 |
CA2703161C (en) | 2011-12-20 |
AU1218100A (en) | 2000-05-15 |
EP1124967A1 (en) | 2001-08-22 |
EP1124967B1 (en) | 2006-05-24 |
ES2327948T3 (es) | 2009-11-05 |
AU768246B2 (en) | 2003-12-04 |
ATE327333T1 (de) | 2006-06-15 |
WO2000024904A1 (en) | 2000-05-04 |
AR020944A1 (es) | 2002-06-05 |
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