KR100983791B1 - Composition of feed additive for livestock - Google Patents
Composition of feed additive for livestock Download PDFInfo
- Publication number
- KR100983791B1 KR100983791B1 KR1020100055234A KR20100055234A KR100983791B1 KR 100983791 B1 KR100983791 B1 KR 100983791B1 KR 1020100055234 A KR1020100055234 A KR 1020100055234A KR 20100055234 A KR20100055234 A KR 20100055234A KR 100983791 B1 KR100983791 B1 KR 100983791B1
- Authority
- KR
- South Korea
- Prior art keywords
- parts
- weight
- livestock
- feed additive
- weight parts
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Inorganic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
Abstract
Description
본 발명은 가축용 사료첨가제 조성물에 관한 것으로, 보다 상세하게는 회향, 도토리, 자단, 계피 및 감초에서 나오는 껍질 및 줄기와 같은 한방부산물과 맥반석, 게르마늄 및 세리사이트의 광물질을 분말화하여 축산업 분야에서 사용되는 통상의 제조사료에 배합하여 사용할 수 있는 가축용 사료첨가제 조성물에 관한 것이다. 본 발명에 따른 사료첨가제는 가축과 그 육제품을 섭취하여 해를 끼칠 수 있는 항생제의 첨가 없이도 육질을 향상시킬 수 있는 가축용 사료첨가제 조성물을 제공하는 것을 특징으로 한다. The present invention relates to a feed additive composition for livestock, and more particularly, in the livestock industry by powdering the minerals of herbal by-products such as shells and stems from fennel, acorns, rosewood, cinnamon and licorice and ganban stone, germanium and sericite It relates to a livestock feed additive composition that can be used in combination with a conventional manufacturer's feed used. The feed additive according to the present invention is characterized by providing a feed additive composition for livestock which can improve meat quality without the addition of antibiotics, which can harm livestock and meat products thereof.
종래 축산가에서는 사료에 항생제나 항균제 등의 다양한 첨가물을 투입하여 건강식 육제품을 생산하기 위해 노력했으나, 사료에 투입된 항생제는 최소 60일 이상이 지나야 완전히 소화배출이 가능하나 이러한 휴약기간 전에 출하 식용될 경우 가축내의 잔여 항생제로 인하여 그 육제품을 섭취한 인체의 면역력을 저하시킬 수 있는 문제점이 있었다. In the past, livestock farmers tried to produce healthy meat products by adding various additives such as antibiotics or antimicrobial agents to the feed, but antibiotics added to the feed can be completely digested and discharged before at least 60 days. Due to the residual antibiotics in the livestock, there was a problem that can reduce the immune system of the human body ingested the meat product.
또한 항생제에 대한 내성을 갖는 세균에서 내성을 함유한 유전물질인 플라스미드의 접합이라는 전달에 의해 다른 세균으로 전이될 수 있으며, 이는 살모넬라균과 식중독균 등의 발생을 유발시킬 수 있는 치명적인 문제로 작용하게 된다. 이러한 이유들로 인해 최근에는 육제품 내의 항생제 잔류와 함께 항생제 내성균에 대한 문제가 제기되면서 항생제 대체 및 세균발생 억제력을 보유하는 물질과 항생제 대체물질로서의 가축 체내 면역기능을 활성화시키는 물질에 대한 연구가 활발히 진행되고 있다.In addition, the transfer of plasmid, which is a genetic material containing resistance, to bacteria that are resistant to antibiotics can be transferred to other bacteria, which is a fatal problem that can cause the occurrence of Salmonella and food poisoning bacteria. . For these reasons, in recent years, the issue of antibiotic-resistant bacteria with antibiotic residues in meat products has been raised, and researches on substances that have antibiotic replacement and bacteriostatic activity and substances that activate the immune system in animals as antibiotic substitutes have been actively conducted. It's going on.
그 중의 한 예로 사료에 광물질을 첨가하는 연구를 볼 수 있는데, 육계사료내에 규산염 광물질을 첨가하여 사육했을 경우 성장개선효과 및 육질개선의 효과에 대해 보고하고 있으며(『사료 내 규산염 광물질 첨가 급여가 육계 생산성과 장내 균총에 미치는 영향』-안승민외 7명, 한국가금학회지 제33권 제1호(2006.03) pp.25-32), 황토광물 중의 대표적인 일라이트를 사료에 첨가하여 돼지에 급여했을 경우 돈육질이 향상된 연구결과(『황토성분(illite) 첨가 급여가 비육돈의 육질에 미치는 영향』-김천제, 한국축산식품학회지 제20권 제2호(2000.06) pp.152-158)도 보고되고 있다.One example is the study of adding minerals to feed, and reports on the effects of growth and meat improvement when broilers are fed with silicate minerals in broiler feeds. Productivity and Influence on Intestinal Bacterium ”-Ahn Seung-min et al., The Korean Society of Poultry Science, Vol. 33, No. 1 (2006.03) pp.25-32). The results of the study on the improvement of meat quality (『Influence of illite supplementation on the meat quality of finishing pigs』 -Kim Cheon-je, Korean Society for Food Science and Nutrition Vol. 20 No. 2 (2000.06) pp.152-158) have been reported.
또 하나의 예로 사료에 한방부산물을 첨가하여 육계품질에 미치는 영향에 관한 연구(『항생제 대체를 위한 비타민 E 및 한방부산물을 포함한 허브추출물 및 효소제 복합처리가 거세돈의 혈액콜레스테롤 및 식육의 품질에 미치는 영향』-강석남외 5명, 한국축산식품학회지 제27권 제1호(2007.03) pp.87-94) 도 보고된 바가 있는데, 이 연구결과에 따르면 한방부산물을 사료에 첨가하여 돼지에 급여한 후 항생물질에 대해 조사한 바, 천연식물 및 한약재에 포함된 다양한 phenolic화합물, alaloids류, flavonoids, glycosides, tannins, spoins 등을 함유하여 이들이 항생기능을 발휘함으로써 항생제 대체물질로의 이용가능성을 제시하고 있다.Another study on the effect of dietary herbal by-products on broiler quality (`` Herbal Extract and Enzyme Combination Treatment with Antibiotics for Vitamin E and Herbal By-products on the Quality of Blood Cholesterol and Meat of Impacts] -Kang Seok-Nam and 5 others, Journal of Korean Society for Food Science of Animal Resources, Vol. 27, No. 1 (2007.03) pp.87-94). According to the results of this study, Korean herbal by-products were added to feed and fed to pigs. Investigating antibiotics, it contains various phenolic compounds, alaloids, flavonoids, glycosides, tannins, spoins, etc. in natural plants and herbal medicines, suggesting the possibility of using them as an alternative to antibiotics.
상기에서 예시한 선행문헌의 문제점에 착안하여 본 발명을 안출한 것으로서, 한방부산물과 광물질을 분말화하여 축산업 분야에서 사용되는 통상의 기초사료에 배합하여 사용함으로써 항생제 내성 세균의 발생을 억제하면서도 육질개선 효과가 있는 가축용 사료첨가제 조성물을 제공하는 것을 목적으로 한다. The present invention has been devised in view of the problems of the prior arts exemplified above, and it improves meat quality while suppressing the occurrence of antibiotic resistant bacteria by blending herbal by-products and minerals and using them in common basic feeds used in the livestock industry. It is an object to provide a feed additive composition for livestock having an effect.
상기 목적을 달성하기 위해 본 발명에 따른 가축용 사료첨가제 조성물은 회향 100 중량부에 대해 도토리 90 내지 95 중량부, 계피 45 내지 50 중량부, 자단 15 내지 20 중량부, 감초 15 내지 20 중량부, 세리사이트 30 내지 35 중량부, 맥반석 5 내지 10 중량부 및 게르마늄 5 내지 10 중량부를 포함하여 이루어지며, 한방부산물인 회향, 도토리, 자단, 계피 및 감초에서 얻어지는 껍질 및 줄기는 100∼150mesh의 입도크기로 분쇄하고, 광물질인 세리사이트, 맥반석 및 게르마늄은 1100∼1300℃의 고온에서 소성시켜 300∼330mesh의 입도크기로 분쇄하여 구성하는 것을 특징으로 한다.Animal feed additive composition according to the present invention for achieving the above object is 90 to 95 parts by weight of acorn, 45 to 50 parts by weight of cinnamon, 15 to 20 parts by weight of rosewood 15 to 20 parts by weight, based on 100 parts by weight of fennel, It consists of 30 to 35 parts by weight of sericite, 5 to 10 parts by weight of ganban stone and 5 to 10 parts by weight of germanium. The skin and stem obtained from the herbal by-products of fennel, acorn, rosewood, cinnamon and licorice have a particle size of 100 to 150 mesh. Crushite, minerals sericite, elvan and germanium are calcined at a high temperature of 1100 ~ 1300 ℃ and pulverized to a particle size of 300 ~ 330mesh.
본 발명의 가축용 사료첨가제 조성물은 통상의 가축용 기초사료 100 중량부에 대해 5 내지 15 중량부를 첨가하여 사용할 수 있으며, 본 발명에서 채용하는 통상의 가축용 기초사료는 시중에서 판매되고 있는 가축용 기초사료로서 조단백질 14.00%, 라이신 0.70%, 메티오닌 0.23%, 칼슘 0.60%, 인 0.50% 이상의 성분을 갖는 기초사료가 적당하며 시판되는 통상의 가축용 기초사료이면 어느 것을 사용해도 무방하다.The feed additive composition for livestock of the present invention may be used by adding 5 to 15 parts by weight with respect to 100 parts by weight of conventional livestock feed, and the general feed for livestock employed in the present invention is used for livestock sold in the market. As a basic feed, a basic feed having a composition of 14.00% crude protein, 0.70% lysine, 0.23% methionine, 0.60% calcium, and 0.50% phosphorus is suitable, and any commercially available basic animal feed may be used.
본 발명에 따른 가축용 사료첨가제 조성물은 항생제의 첨가 없이도 영양이 우수하면서도 육질을 개선시킬 수 있는 효과를 제공한다.The feed additive composition for livestock according to the present invention provides the effect of improving the meat quality while providing excellent nutrition without the addition of antibiotics.
이하 본 발명을 실시예와 비교예를 들어 구체적으로 설명하고자 하나, 본 발명의 범위가 하기 실시예에 의해 한정되는 것은 아니다.
Hereinafter, the present invention will be described in detail with reference to Examples and Comparative Examples, but the scope of the present invention is not limited by the following Examples.
[실시예][Example]
실시예 1Example 1
회향, 도토리, 자단, 계피 및 감초의 껍질과 줄기를 4∼5cm 크기로 절단하여 열풍건조기에 투입하여 90℃에서 24시간 건조시킨 후, 150mesh의 입도크기로 분쇄하여 분말화하였다.The skins and stems of fennel, acorn, rosewood, cinnamon and licorice were cut into 4-5 cm in size, put into a hot air dryer, dried at 90 ° C. for 24 hours, and then pulverized to a size of 150 mesh to be powdered.
세리사이트, 맥반석 및 게르마늄을 1300℃의 고온에서 소성시켜 300mesh의 입도크기로 분쇄하여 분말화하였다.Sericite, elvan and germanium were calcined at a high temperature of 1300 ° C. and pulverized to a particle size of 300 mesh to be powdered.
회향 100 중량부에 대해 도토리 95 중량부, 계피 45 중량부, 자단 15 중량부, 감초 15 중량부, 세리사이트 30 중량부, 맥반석 5 중량부 및 게르마늄 5 중량부를 혼합하여 가축용 사료첨가제 조성물을 제조하였다.95 parts by weight of acorns, 45 parts by weight of cinnamon, 15 parts by weight of rosewood, 15 parts by weight of licorice, 30 parts by weight of sericite, 5 parts by weight of ganban stone and 5 parts by weight of germanium to prepare a feed additive composition for livestock It was.
시판되고 있는 통상의 가축용 기초사료(국내 한양사료공업사 제조. 제품명 SEW -조단백질 14.00%, 라이신 0.70%, 메티오닌 0.23%, 칼슘 0.60%, 인 0.50% 포함) 100 중량부에 대해 상기 사료첨가제 조성물 10 중량부를 첨가하여 돼지 급여용 사료를 완성하였다.
The feed additive composition 10 based on 100 parts by weight of conventional commercial feed for domestic livestock (manufactured by Hanyang Feed, Inc., product name: SEW-crude protein 14.00%, lysine 0.70%, methionine 0.23%, calcium 0.60%, phosphorus 0.50%) The parts were added to complete the pig feed.
상기 실시예1과 비교하기 위한 대조군으로 비교예1과 비교예2를 아래와 같이 준비하였다.
Comparative Example 1 and Comparative Example 2 were prepared as follows as a control for comparing with Example 1.
[비교예][Comparative Example]
비교예1Comparative Example 1
실시예1에서 사용한 시판되고 있는 통상의 가축용 기초사료(국내 한양사료공업사 제조. 제품명 SEW -조단백질 14.00%, 라이신 0.70%, 메티오닌 0.23%, 칼슘 0.60%, 인 0.50% 포함)
Commercial commercial feed for livestock used in Example 1 (manufactured by Hanyang Feed Industry Co., Ltd., product name: SEW-crude protein 14.00%, lysine 0.70%, methionine 0.23%, calcium 0.60%, phosphorus 0.50%)
비교예2Comparative Example 2
실시예1에서 사용한 시판되고 있는 통상의 가축용 기초사료(국내 한양사료공업사 제조. 제품명 SEW -조단백질 14.00%, 라이신 0.70%, 메티오닌 0.23%, 칼슘 0.60%, 인 0.50% 포함) 100 중량부에 대해 flavomycin 항생물질 0.5 중량부를 첨가하여 제조한 돼지 급여용 사료.
For 100 parts by weight of a commercial livestock basic feed (manufactured by Hanyang Feed Industry Co., Ltd., product name SEW-crude protein 14.00%, lysine 0.70%, methionine 0.23%, calcium 0.60%, phosphorus 0.50%) used in Example 1 Feed for pigs prepared with 0.5 parts by weight of flavomycin antibiotics.
[실험예][Experimental Example]
상기 실시예1과 비교예1 및 2를 초기 평균 무게가 50.48kg인 총 40두의 3원 교잡종 거세돼지(Landrace x Yorkshire x Duroc)를 69일간 급여하여 사양한 후 혈액을 채취하여 실험예1의 혈중콜레스테롤 분석에 사용하였으며, 도축 24시간 이후에 -20℃에서 실험 전까지 냉동보관 이후에 실험예2, 3 및 4의 시료로 사용하였다.
Example 1 and Comparative Examples 1 and 2 were fed by feeding a total of 40 headed three-way hybrid castrated pigs (Landrace x Yorkshire x Duroc) having an initial average weight of 50.48 kg for 69 days, and then collecting blood. It was used for blood cholesterol analysis, and used as a sample of Experimental Examples 2, 3, and 4 after freezing storage until the experiment at -20 ℃ after 24 hours of slaughter.
실험예1 혈중콜레스테롤 분석Experimental Example 1 Analysis of Blood Cholesterol
1mL 혈액을 2,000rpm으로 20분간 원심분리하여 혈청을 분리하여 실험에 이용하였으며, 분리한 혈청은 -20℃에서 보관하였다가 상업적 kit(Total Cholesterol E-Test Wako, Wako Pure Chemical, Osaka, Japan)를 이용하여 HDL-콜레스테롤 및 LDL-콜레스테롤을 분석하여 아래 [표1]에 나타내었다. HDL-콜레스테롤의 경우, 실시예1에서 함량이 가장 높게 나왔으며, LDL-콜레스테롤의 경우 비교예1에서 함량이 더 높게 나타났다. 통상적으로 HDL-콜레스테롤은 높고, LDL-콜레스테롤의 함량이 낮을수록 우수한 육질로 판단하고 있는데, 실시예1의 경우 돈육의 육질개선에 영향을 끼쳤음을 확인할 수 있었다.1mL blood was centrifuged at 2,000rpm for 20 minutes to separate serum and used for experiment. The separated serum was stored at -20 ℃ and commercial kit (Total Cholesterol E-Test Wako, Wako Pure Chemical, Osaka, Japan) was used. HDL-cholesterol and LDL-cholesterol were analyzed using Table 1 below. In case of HDL-cholesterol, the content was highest in Example 1, and in case of LDL-cholesterol, the content was higher in Comparative Example 1. In general, the higher the HDL-cholesterol, the lower the content of LDL-cholesterol is judged to be excellent meat quality, in the case of Example 1 it was confirmed that it affected the meat quality of pork.
LDL-CholesterolHDL-Cholesterol
LDL-Cholesterol
67.11±0.3369.48 ± 0.82
67.11 ± 0.33
148.53±0.8233.15 ± 0.21
148.53 ± 0.82
112.35±4.5941.01 ± 1.00
112.35 ± 4.59
실험예2 근육내 비타민E 함량Experimental Example 2 Vitamin E Content in Muscle
비타민E의 분석은 Buttriss와 Diplock의 방법을 변형하여 실시하였다. 2g의 돈육등심을 9.0mL 1.2% KCL(w/v)로 혼합후 균질화하였다. 2mL 균질액을 취하여 2.0mL 1.0% pyrogallol(in absolute ethanol)과 혼합하였다. 이후 0.5mL 50% KOH(w/v)을 혼합하고 70℃ 수조에서 30분간 가열하여 saponification을 하였다. 이후 ice에서 냉각하고 0.001% BHT를 함유한 4mL hexane로 2회 이상 회수하였다. 회수 이후 질소를 이용해 flushing하였고 다시 1.0mL hexane에 녹여 분석을 위한 vial에 넣어 2010pump(Jasco Europe S.R.L., Como, Italy)가 장착된 HPLC(Jasco Europ S.R.L., Como, Italy) system을 이용하여 분석하였다. HPLC의 분석조건은 UV 292nm, 이동상 methanol:water(97:3 v/v), 컬럼 C18 column(Hewlett Packard RP18)), 그리고 유속 1.0mL/min였다. 하기 [표2]를 보면, 실시예1의 비타민E 함량이 비교예1보다 5배 이상 높게 그리고 비교예2보다는 3배 정도 넘게 높게 나타났다.The analysis of vitamin E was carried out by modifying the Buttriss and Diplock methods. 2 g of pork sirloin was homogenized after mixing with 9.0 mL 1.2% KCL (w / v). A 2 mL homogenate was taken and mixed with 2.0 mL 1.0% pyrogallol (in absolute ethanol). Then 0.5mL 50% KOH (w / v) was mixed and heated for 30 minutes in a 70 ℃ water bath was saponification. Thereafter, the mixture was cooled on ice and recovered two more times with 4mL hexane containing 0.001% BHT. After recovery, the mixture was flushed with nitrogen, dissolved in 1.0 mL hexane, and put into a vial for analysis, using an HPLC (Jasco Europ S.R.L., Como, Italy) system equipped with a 2010pump (Jasco Europe S.R.L., Como, Italy). HPLC analysis conditions were UV 292 nm, mobile phase methanol: water (97: 3 v / v), column C18 column (Hewlett Packard RP18), and flow rate 1.0 mL / min. In the following [Table 2], the vitamin E content of Example 1 was more than five times higher than Comparative Example 1 and more than three times higher than Comparative Example 2.
실험예 3 지방 산패도(TBARS) 및 단백질 변패도(VBN)Experimental Example 3 Fatty Acid Saturation (TBARS) and Protein Morphology (VBN)
지방 산패도(TBARS)는 돈육 냉동저장 7일차에 Witte Krause방법에 의해 측정하여 mg malonaldehyde/kg으로 표시하였으며, 단백질 변패도(VBN)는 Conway방법에 따라 분석하여 아래 [표3]에 나타내었다. 실시예1에서는 0.04mg MA/kg의 TBARS 및 7.62 mg%의 VBN을 가져 비교예1 및 비교예2에 비해 신선도 및 저장성이 우수함을 확인할 수 있었다.Fatty acidity (TBARS) was measured in mg malonaldehyde / kg by the Witte Krause method on the 7th day of freezing storage of pork, and protein variability (VBN) was analyzed according to Conway method and is shown in [Table 3] below. In Example 1 it was confirmed that the TBARS of 0.04 mg MA / kg and VBN of 7.62 mg% has excellent freshness and storage capacity compared to Comparative Examples 1 and 2.
VBN(mg%)TBARS (mg MA / kg)
VBN (mg%)
7.62±0.130.04 ± 0.01
7.62 ± 0.13
22.23±0.081.18 ± 0.10
22.23 ± 0.08
15.07±0.660.51 ± 0.05
15.07 ± 0.66
실험예 4 잔류항생물질 분석Experimental Example 4 Residual Antibiotic Analysis
Tetracyline계열의 항생물질 분석Tetracyline family antibiotic analysis
시료 튜브에 1g의 돈육 등심 시료와 0.1g Oxalic acid와 0.1g EDTA를 넣고 균질화 시켰다. 10분간 소니케이트를 실시한 이후 2분간 voltex를 실시하여 얻은 추출물은 다시 5mL acetonitrile/acetate(2:1)와 균질화하였다. 1g of pork fillet sample, 0.1g oxalic acid and 0.1g EDTA were added to the sample tube and homogenized. After extracting for 10 minutes by sonicating for 2 minutes, the extract was homogenized with 5 mL acetonitrile / acetate (2: 1).
균질화 이후 5,000rpm에서 20분간 원심분리하고 상층액을 취해 질소로 flushing하였다. 위의 과정을 3회 반복 회수하였고, 이후 2mL 이동상을 첨가하고 2,000rmp에서 2분간 원심분리 이후 상층액을 0.45μm filter로 필터하여 2010pump(Jasco Europe S.R.L., Como, Italy)가 장착된 HPLC(Jasco Europ S.R.L., Como, Italy) system을 이용하여 분석하였다. HPLC의 분석조건은 UV 360nm, 이동상 0.01M oxalic acid/acetonitrile(85/15), 컬럼 Xterra C18 column(30℃), 그리고 유속 1.0mL/min였다. 항생물질의 표준폼은 sulfamethazine, sulfamerazine, sulfadimethoxine, sulfamonomethoxine 그리고 sulfaquinoxaline이었다.
After homogenization, centrifugation was performed at 5,000 rpm for 20 minutes, and the supernatant was taken out and flushed with nitrogen. The above procedure was repeated three times, and then a 2 mL mobile phase was added, followed by centrifugation at 2,000 rmp for 2 minutes, and the supernatant was filtered with a 0.45 μm filter, which was equipped with an HPLC (Jasco Europ, Jasco Europe SRL, Como, Italy). SRL, Como, Italy) system. HPLC analysis conditions were UV 360nm, mobile phase 0.01M oxalic acid / acetonitrile (85/15), column Xterra C18 column (30 ° C), and flow rate 1.0mL / min. Standard forms of antibiotics were sulfamethazine, sulfamerazine, sulfadimethoxine, sulfamonomethoxine and sulfaquinoxaline.
Sulfa계열의 항생물질 분석Antibiotic Analysis of Sulfa Family
시료 튜브에 1g의 돈육 등심 시료와 1mL 0.1% KH2PO4를 섞은 이후 5분간 균질화 이후 8mL acetonitril로 30분간 균질화하였다. 균질화 이후 4,000rpm에서 15분간 원심분리 이후 상층액을 취했고 위의 과정을 3회 반복 회수하였다. 회수한 상층액은 acetonitrile로 포화시킨 15mL hexane과 혼합하여 질소로 flushing 이후 추출물을 1mL 50% acetonitrile과 1mL 증류수에 녹인 후 12,000rpm에서 30분간 원심분리하였다. 원심분리 후 상층액을 0.45μm filter로 필터하여 2010pump(Jasco Europe S.R.L., Como, Italy)가 장착된 HPLC(Jasco Europ S.R.L., Como, Italy) system을 이용하여 분석하였다. HPLC의 분석조건은 UV 270nm, 이동상 0.1% K2HPO4(pH 3.5)/acetonitrile(84/16), 컬럼 Novapec C18 column(30℃), 그리고 유속 1.0mL/min였다. 항생물질의 표준폼은 tetracycline, oxytetracycline, chlortetracycline 그리고 ampicillim이었다.
After mixing 1 g of pork fillet sample and 1 mL 0.1% KH 2 PO 4 in the sample tube, the mixture was homogenized for 5 minutes and then homogenized with 8 mL acetonitril for 30 minutes. After homogenization, the supernatant was taken after centrifugation at 4,000 rpm for 15 minutes, and the above procedure was recovered three times. The recovered supernatant was mixed with 15 mL hexane saturated with acetonitrile, flushed with nitrogen, dissolved in 1 mL 50% acetonitrile and 1 mL distilled water, and then centrifuged at 12,000 rpm for 30 minutes. After centrifugation, the supernatant was filtered with a 0.45 μm filter and analyzed using an HPLC (Jasco Europ SRL, Como, Italy) system equipped with a 2010 pump (Jasco Europe SRL, Como, Italy). HPLC analysis conditions were UV 270 nm, mobile phase 0.1% K 2 HPO 4 (pH 3.5) / acetonitrile (84/16), column Novapec C18 column (30 ° C.), and flow rate 1.0 mL / min. Standard forms of antibiotics were tetracycline, oxytetracycline, chlortetracycline and ampicillim.
상기 실시예1과 비교예1 및 2를 대상으로 항생물질(antibiotics)에 대한 잔류측정값을 아래 [표4]에 나타내었다. 국내 항생물질의 잔류허용기준은 0.1ppm 이하이며, 비교예2에서는 sulfa-계열의 항생물질은 검출되지 않았으나 tetracyline계열의 항생물질인 Oxytetracycline과 Chlortetracycline에서 국내 허용기준범위인 0.06±0.01과 0.08±0.01ppm이 검출되었고, 실험예1 및 비교예1에서는 어떠한 항생물질도 검출되지 않았다. Residual measurement values for antibiotics (antibiotics) for Example 1 and Comparative Examples 1 and 2 are shown in Table 4 below. The residual limit of antibiotics in Korea is 0.1ppm or less. In Comparative Example 2, sulfa-based antibiotics were not detected, but tetracyline-based antibiotics Oxytetracycline and Chlortetracycline, 0.06 ± 0.01 and 0.08 ± 0.01ppm Was detected, and no antibiotics were detected in Experimental Example 1 and Comparative Example 1.
Sulfamerazine
Sulfadimethoxine
Sulfamonomethoxine
Sulfaquinoxaline
Tetracycline
Oxytetracycline
Chlortetracycline
AmpicillinSulfamethazine
Sulfamerazine
Sulfadimethoxine
Sulfamonomethoxine
Sulfaquinoxaline
Tetracycline
Oxytetracycline
Chlortetracycline
Ampicillin
nd
nd
nd
nd
nd
nd
nd
ndnd 1)
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
ndnd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
0.06±0.01
0.08±0.01
ndnd
nd
nd
nd
nd
nd
0.06 ± 0.01
0.08 ± 0.01
nd
Not detectNot detect
Claims (5)
90 to 95 parts by weight of acorns, 45 to 50 parts by weight of cinnamon, 15 to 20 parts by weight of rosewood, 15 to 20 parts by weight of licorice, 30 to 35 parts by weight of sericite, 5 to 10 parts by weight of gantherite and germanium Livestock feed additive composition comprising 5 to 10 parts by weight.
The feed additive composition for livestock according to claim 1, wherein the sericite, elvan and germanium are calcined at a temperature of 1100 to 1300 ° C.
The animal feed additive composition according to claim 1, wherein the fennel, acorn, rosewood, cinnamon and licorice are pulverized to a particle size of 100 to 150 mesh.
The livestock feed additive composition according to claim 2, wherein the sericite, elvan and germanium are pulverized to a particle size of 300 to 330 mesh.
The animal feed additive composition according to any one of claims 1 to 4, wherein the animal feed additive composition is used by adding 5 to 15 parts by weight with respect to 100 parts by weight of a general animal feed. .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020100055234A KR100983791B1 (en) | 2010-06-11 | 2010-06-11 | Composition of feed additive for livestock |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020100055234A KR100983791B1 (en) | 2010-06-11 | 2010-06-11 | Composition of feed additive for livestock |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR100983791B1 true KR100983791B1 (en) | 2010-09-28 |
Family
ID=43010401
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020100055234A KR100983791B1 (en) | 2010-06-11 | 2010-06-11 | Composition of feed additive for livestock |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR100983791B1 (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101165933B1 (en) | 2011-12-20 | 2012-07-19 | 박시내 | Feed composition for pig breeding comprising cinnammons |
| KR20170131999A (en) | 2016-05-23 | 2017-12-01 | 농업회사법인 주식회사 산드레 | Pig feed composition including the Acorn, a breeding method using the same and pork obtained therefrom |
| KR101807800B1 (en) | 2017-04-27 | 2017-12-13 | 주식회사 바이오테크 | Additives for stock feed comprising |
| KR20200103230A (en) | 2019-02-22 | 2020-09-02 | 박대현 | Method for the manufacture of probiotics, feed additive for herbivores with the main ingredient of [jangdae], a new kind of Kenaf |
| KR20220005262A (en) | 2020-07-06 | 2022-01-13 | 진강중 | Feed composition for livestock containing sericite |
| KR20220005263A (en) | 2020-07-06 | 2022-01-13 | 진강중 | Feed composition for comoanion animals containing sericite |
| KR102389589B1 (en) | 2021-09-29 | 2022-04-22 | 진지영 | Antifungal composition comprising spirits extract of Lespedeza cuneata and process for preparing the same |
| KR20230054016A (en) | 2021-10-15 | 2023-04-24 | 진지영 | Antiseptic composition and process for preparing the same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020024176A (en) * | 2002-01-30 | 2002-03-29 | 손영석 | Manufacturing method of feed containing substance becoming medicine |
| KR100330751B1 (en) | 1999-06-10 | 2002-04-03 | 박준호 | The chicken feed manufacture method which uses re- Chinese medicine and feed use method |
| KR100914085B1 (en) | 2007-07-27 | 2009-08-27 | 정명철 | Method of making freshwater snail feed with snail shell |
-
2010
- 2010-06-11 KR KR1020100055234A patent/KR100983791B1/en not_active IP Right Cessation
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100330751B1 (en) | 1999-06-10 | 2002-04-03 | 박준호 | The chicken feed manufacture method which uses re- Chinese medicine and feed use method |
| KR20020024176A (en) * | 2002-01-30 | 2002-03-29 | 손영석 | Manufacturing method of feed containing substance becoming medicine |
| KR100914085B1 (en) | 2007-07-27 | 2009-08-27 | 정명철 | Method of making freshwater snail feed with snail shell |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101165933B1 (en) | 2011-12-20 | 2012-07-19 | 박시내 | Feed composition for pig breeding comprising cinnammons |
| KR20170131999A (en) | 2016-05-23 | 2017-12-01 | 농업회사법인 주식회사 산드레 | Pig feed composition including the Acorn, a breeding method using the same and pork obtained therefrom |
| KR101807800B1 (en) | 2017-04-27 | 2017-12-13 | 주식회사 바이오테크 | Additives for stock feed comprising |
| KR20200103230A (en) | 2019-02-22 | 2020-09-02 | 박대현 | Method for the manufacture of probiotics, feed additive for herbivores with the main ingredient of [jangdae], a new kind of Kenaf |
| KR20220005262A (en) | 2020-07-06 | 2022-01-13 | 진강중 | Feed composition for livestock containing sericite |
| KR20220005263A (en) | 2020-07-06 | 2022-01-13 | 진강중 | Feed composition for comoanion animals containing sericite |
| KR102389589B1 (en) | 2021-09-29 | 2022-04-22 | 진지영 | Antifungal composition comprising spirits extract of Lespedeza cuneata and process for preparing the same |
| KR20230054016A (en) | 2021-10-15 | 2023-04-24 | 진지영 | Antiseptic composition and process for preparing the same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100983791B1 (en) | Composition of feed additive for livestock | |
| CN101095460B (en) | Tea polyphenol composite antioxidant for feedstuff and method for preparing the same | |
| Yigit et al. | Effects of cellulase addition to canola meal in tilapia (Oreochromis niloticus L.) diets | |
| JP6462887B2 (en) | A drinking agent and a feed composition having a function of removing malodor from pet urine, comprising a mixture of natural extracts as an active ingredient | |
| RU2356240C2 (en) | Fodder and supplement containing alkaloid | |
| Kesbiç et al. | Structural and chemical changes of grape seed extract after thermal processing and its use in rainbow trout (Oncorhynchus mykiss) diets as an organic feed supplement | |
| Hamady et al. | Effect of Pomegranate peel extract as natural growth promoter on the productive performance and intestinal microbiota of broiler chickens | |
| Agubosi et al. | Influence of dietary inclusion of Sunflower (Helianthus annus) oil on growth performance and oxidative status of broiler chicks | |
| EP2385765A1 (en) | Additive to food or drinking water containing botanical antioxidants together with probiotic microorganisms | |
| Abdulsalam et al. | Performance of broiler chickens fed on Moringa oleifera leaf meal supplemented poultry feed | |
| Kim et al. | Effect of fermented ginkgo biloba and camelia sinensis-based probiotics on growth performance, immunity and caecal microbiology in broilers | |
| Essien et al. | Effect of Aridan (Tetrapleura tetraptera) powder as feed additive on the performance and nutrient digestibility of broiler chickens | |
| KR101151879B1 (en) | Functional feed comprising mulberry leaves, and a method for preparing the same | |
| KR101168632B1 (en) | A feed composition | |
| Uushona et al. | Ruminant meat production and quality enhancement, nematode suppression and greenhouse gas emission mitigation: A sustainable paradigm for valorisation of Acacia leaves | |
| AYDIN et al. | The effects of thyme oil and black cumin oil in broiler feeding on growth performance, intestinal histomorphology, and cecal volatile fatty acids | |
| Ahmad et al. | Effects of replacing antibiotic growth promoters (AGPS) with botanical extracts and oils in feed of laying hens on production, performance and some microbial counts in feces. | |
| KR20150014150A (en) | Feed for livestock comprising allium hookeri extract or its fermented product and use thereof | |
| Rajaian et al. | Satureja hortensis as a growth promoter in broiler chickens | |
| KR101273276B1 (en) | Method for producing feed for livestock adding curcumin and feed for livestock produced by the same | |
| KR20180114118A (en) | Functional food | |
| Cruz Villeda | Effect of dietary essential oils supplementation on growth performance, protein digestibility and digestive enzymes in juvenile gilthead seabream fed a low fishmeal diet | |
| KR100680308B1 (en) | The manufacturing method of feed additives using mugwort or dioscoreaceae rhizoma added licorice extract, the feed additives therefrom and hanwoo beef produced by the said feed additives | |
| JP2008509097A (en) | Use of Button genus and its extract with growth promoting activity in veterinary and animal husbandry | |
| KR102321162B1 (en) | A feed supplement composition for reducing zinc oxide of feed comprising Phellodendron Cortex extract as an effective ingredient |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| A302 | Request for accelerated examination | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant | ||
| LAPS | Lapse due to unpaid annual fee |