JP4625698B2 - 循環マクロファージの決定および/または分類方法ならびにその方法を実施するための分析装置 - Google Patents
循環マクロファージの決定および/または分類方法ならびにその方法を実施するための分析装置 Download PDFInfo
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- JP4625698B2 JP4625698B2 JP2004548880A JP2004548880A JP4625698B2 JP 4625698 B2 JP4625698 B2 JP 4625698B2 JP 2004548880 A JP2004548880 A JP 2004548880A JP 2004548880 A JP2004548880 A JP 2004548880A JP 4625698 B2 JP4625698 B2 JP 4625698B2
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- cells
- macrophages
- antigen
- prostate
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57434—Specifically defined cancers of prostate
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physiology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Ecology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
PSA抗体Ab-1(クローンER-PRS)
パンサイトケラチン-FITC
上皮膜抗原(クローンE29)
アイソタイプコントロールIgG1(クローンDAK-GO1)
二次抗体FITCヤギ抗マウス(DAKO)。
Claims (5)
- 循環マクロファージの特徴を決定し、かつ/または分類する方法であって、
・採取した全血試料を、勾配遠心分離を行ってマクロファージを単離する工程、
・該マクロファージ細胞を穿孔処理する工程、
・該細胞を、前立腺特異抗原(PSA)、サイトケラチンおよび/または上皮膜抗原と、抗原抗体反応する少なくとも1つの選択された抗体で、細胞内染色する工程、および
・該前処理済み細胞のフローサイトメトリー分析を行い、続いて複数の細胞の統計学的評価を行う工程、を含む方法。 - フローサイトメトリーを実施した後にアイソタイプコントロールと染色のヒストグラム分析を行うことを特徴とする、請求項1の方法。
- 散在性前立腺腫瘍の貧食によって取り込まれた組織細胞の一部を人体外で検出するための請求項1または2に記載の方法。
- 貧食によって取り込まれた前記物質が前立腺関連であるかどうかを、前記マクロファージにおける前立腺特異抗原(PSA)の前記染色によって決定することを特徴とする、請求項3の方法。
- 請求項1に記載の循環マクロファージの特徴を決定し、かつ/または分類する方法を実施するための分析装置であって、
採取した血液をヘパリン処理するための手段、
マクロファージを単離するための勾配遠心分離器、
該マクロファージ細胞を穿孔処理する細胞穿孔手段、
前記前処理済み細胞を前立腺特異抗原(PSA)、サイトケラチンおよび/または上皮膜抗原と、抗原抗体反応する少なくとも1つの選択された抗体で、細胞内染色するための機器、および
腫瘍の初期診断を目的として単離および前処理済み細胞の細胞内構造を決定するためのコンピュータ支援評価ユニットを装備したフローサイトメーターを備える分析装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10228548 | 2002-06-26 | ||
DE10230893A DE10230893A1 (de) | 2002-06-26 | 2002-07-09 | Verfahren zur Eigenschaftsbestimmung und/oder Klassifikation von zirkulierenden Macrophagen und/oder peripheren blutmononuclearen Zellen sowie Analyseanordnung zur Durchführung des Verfahrens |
PCT/EP2003/005763 WO2004003548A1 (de) | 2002-06-26 | 2003-06-02 | Verfahren zur eigenschaftsbestimmung und/oder klassifikation von zirkulierenden macrophagen sowie analyseanordnung zur durchführung des verfahrens |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2005531785A JP2005531785A (ja) | 2005-10-20 |
JP4625698B2 true JP4625698B2 (ja) | 2011-02-02 |
Family
ID=30001475
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2004548880A Expired - Fee Related JP4625698B2 (ja) | 2002-06-26 | 2003-06-02 | 循環マクロファージの決定および/または分類方法ならびにその方法を実施するための分析装置 |
Country Status (7)
Country | Link |
---|---|
US (1) | US20060094067A1 (ja) |
EP (1) | EP1516182B1 (ja) |
JP (1) | JP4625698B2 (ja) |
CN (1) | CN1668922A (ja) |
AU (1) | AU2003238193A1 (ja) |
CA (1) | CA2491221C (ja) |
WO (1) | WO2004003548A1 (ja) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090056495A1 (en) * | 2007-08-27 | 2009-03-05 | Todd Bischoff | Bicycle handlebar assembly |
ES2663662T3 (es) * | 2008-01-18 | 2018-04-16 | President And Fellows Of Harvard College | Métodos para detectar distintivos de enfermedades o afecciones en fluidos corporales |
JP2011530077A (ja) * | 2008-08-04 | 2011-12-15 | シンメッド リサーチ ゲーエムベーハー | 組織から循環系に再循環させられる血中マクロファージによって組織から細胞内に吸収される分子マーカーを特徴づける、具体的には定量化する、ための方法 |
WO2012012717A1 (en) | 2010-07-23 | 2012-01-26 | President And Fellows Of Harvard College | Methods of detecting prenatal or pregnancy-related diseases or conditions |
SG10201505724SA (en) * | 2010-07-23 | 2015-09-29 | Harvard College | Methods of detecting diseases or conditions using phagocytic cells |
US20130184178A1 (en) | 2010-07-23 | 2013-07-18 | President And Fellows Of Harvard College | Methods of Detecting Autoimmune or Immune-Related Diseases or Conditions |
EP2495567A1 (en) | 2011-03-04 | 2012-09-05 | Erasmus University Medical Center Rotterdam | Methods and means for monitoring disruption of tissue homeostasis in the total body |
EP2817606B1 (en) * | 2012-02-24 | 2021-06-02 | Instrunor AS | Instrument for pretreating cell samples |
WO2014164366A1 (en) | 2013-03-09 | 2014-10-09 | Harry Stylli | Methods of detecting cancer |
CN107192656A (zh) * | 2017-04-06 | 2017-09-22 | 大连工业大学 | 一种基于流式细胞仪的刺参体腔细胞吞噬率检测方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH11118792A (ja) * | 1997-10-17 | 1999-04-30 | Ajinomoto Co Inc | マクロファージの分類方法、免疫性疾患等の検定方法及び免疫性疾患治療剤のスクリーニング方法 |
DE69912453T2 (de) * | 1998-08-27 | 2004-07-22 | Mayo Foundation For Medical Education And Research, Rochester | Verfahren und materialien zur diagnose instabiler angina |
DE19850049A1 (de) * | 1998-10-30 | 2000-05-11 | Inst Diabetes Gerhardt Katsch | Verfahren zur Bestimmung intrazellulärer Zytokine der Immunzellen in biologischen Körperflüssigkeit und Organ- oder Gewebeproben mittels Durchflußzytometrie |
-
2003
- 2003-06-02 JP JP2004548880A patent/JP4625698B2/ja not_active Expired - Fee Related
- 2003-06-02 AU AU2003238193A patent/AU2003238193A1/en not_active Abandoned
- 2003-06-02 EP EP03735519A patent/EP1516182B1/de not_active Expired - Lifetime
- 2003-06-02 CA CA2491221A patent/CA2491221C/en not_active Expired - Lifetime
- 2003-06-02 WO PCT/EP2003/005763 patent/WO2004003548A1/de active Application Filing
- 2003-06-02 CN CNA038167026A patent/CN1668922A/zh active Pending
- 2003-06-02 US US10/519,174 patent/US20060094067A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2004003548A1 (de) | 2004-01-08 |
JP2005531785A (ja) | 2005-10-20 |
CN1668922A (zh) | 2005-09-14 |
US20060094067A1 (en) | 2006-05-04 |
EP1516182B1 (de) | 2010-08-25 |
CA2491221C (en) | 2013-11-12 |
EP1516182A1 (de) | 2005-03-23 |
CA2491221A1 (en) | 2004-01-08 |
AU2003238193A1 (en) | 2004-01-19 |
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