JP2009115542A - 流路構造、これを備えた流路基板及び流体制御方法 - Google Patents
流路構造、これを備えた流路基板及び流体制御方法 Download PDFInfo
- Publication number
- JP2009115542A JP2009115542A JP2007287337A JP2007287337A JP2009115542A JP 2009115542 A JP2009115542 A JP 2009115542A JP 2007287337 A JP2007287337 A JP 2007287337A JP 2007287337 A JP2007287337 A JP 2007287337A JP 2009115542 A JP2009115542 A JP 2009115542A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- flow path
- flow
- fluid
- axis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012530 fluid Substances 0.000 title claims abstract description 88
- 239000000758 substrate Substances 0.000 title claims description 59
- 238000000034 method Methods 0.000 title claims description 37
- 238000007599 discharging Methods 0.000 claims abstract description 6
- 239000011324 bead Substances 0.000 claims description 23
- 238000005452 bending Methods 0.000 claims description 13
- 239000000523 sample Substances 0.000 description 132
- 238000004458 analytical method Methods 0.000 description 23
- 238000004519 manufacturing process Methods 0.000 description 20
- 239000007788 liquid Substances 0.000 description 16
- 238000005259 measurement Methods 0.000 description 16
- 239000012488 sample solution Substances 0.000 description 16
- 238000004088 simulation Methods 0.000 description 11
- 238000000465 moulding Methods 0.000 description 9
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 8
- 238000001746 injection moulding Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 238000009826 distribution Methods 0.000 description 6
- 230000000704 physical effect Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 238000000684 flow cytometry Methods 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 229920005989 resin Polymers 0.000 description 4
- 239000011347 resin Substances 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000696 magnetic material Substances 0.000 description 2
- 230000005415 magnetization Effects 0.000 description 2
- 239000012508 resin bead Substances 0.000 description 2
- 238000007493 shaping process Methods 0.000 description 2
- 238000004611 spectroscopical analysis Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 1
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 238000002509 fluorescent in situ hybridization Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 238000004452 microanalysis Methods 0.000 description 1
- 239000002088 nanocapsule Substances 0.000 description 1
- 239000007908 nanoemulsion Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229920005992 thermoplastic resin Polymers 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/40—Static mixers
- B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
- B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
- B01F25/432—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction with means for dividing the material flow into separate sub-flows and for repositioning and recombining these sub-flows; Cross-mixing, e.g. conducting the outer layer of the material nearer to the axis of the tube or vice-versa
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
- B01F33/3011—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00851—Additional features
- B01J2219/00858—Aspects relating to the size of the reactor
- B01J2219/0086—Dimensions of the flow channels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00889—Mixing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00925—Irradiation
- B01J2219/0093—Electric or magnetic energy
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00925—Irradiation
- B01J2219/00934—Electromagnetic waves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0636—Focussing flows, e.g. to laminate flows
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0874—Three dimensional network
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/0318—Processes
- Y10T137/0391—Affecting flow by the addition of material or energy
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/8593—Systems
- Y10T137/87571—Multiple inlet with single outlet
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
【解決手段】試料を導入する第1の導入部(11)と、前記試料を挟み込むための流体を導入する第2の導入部(13,17)と、前記試料を排出する排出部(12)と、を少なくとも備えた流路構造であり、前記試料の導入方向をX方向とすると、Y軸周りに略90度に流路が曲折する曲折部(15)と、X軸周りに略90度に流路が曲折する曲折部(16)と、を少なくとも備える流路構造(1)とすること。
【選択図】図1
Description
次に、前記試料の搬送方向に対して前記流路を断面視した形状は、略同じ形状で維持されている流路構造を提供する。断面視した形状が流路内において略同じ形状であることで、試料が流路中で詰まる現象を効果的に防止できる。
また、本発明は、この流路構造を備えた流路基板を提供する。
更に、本発明は、前記試料を流体で挟み込んで流路中を搬送させる際に、順不動で、下記(1)前記試料の導入方向をX方向とすると、前記試料をY軸周りに略90度曲折させる工程と、(2)前記試料をX軸周りに略90度曲折させる工程と、を少なくとも行う流体制御方法を提供する。
そして、本発明は、前記試料又は前記流体の流体条件を制御することで、前記流路中における前記試料の位置制御を行なう流体制御方法を提供する。前記流量を制御することで、流路中を流れる前記試料の位置を制御できる。その結果、より高精度の流体制御が可能となる。
また、本発明は、前記試料は細胞及び/又はビーズを含み、前記細胞及び/又はビーズの前記流路中の位置情報を、前記流路中の所定位置において検出し、該位置情報に基づいて前記位置制御を行なう流体制御方法を提供する。細胞やビーズの位置情報を検出することで、より正確な位置制御を行なうことができる。
図9に示す流路構造のモデルについてコンピュータ解析を行なった。図9は同流路構造のモデルの概略図である。図9の(1)は、流路構造を上面視した状態を示す。図9の(2)は流路構造を右側方から側面視した状態を示す。この図9の流路構造は、3箇所の曲折部を有している(例えば、図1等参照)。なお、図9に示す大きさや形状はシミュレーションの一例として採用したものであり、本発明の流路構造のサイズ等について限定するものではない(以下同様)。
図12に示す流路構造のモデルについてコンピュータ解析を行なった。図12は同流路構造のモデルの概略図である。図12の(1)は、流路構造を上面視した状態を示す。図12の(2)は流路構造を右側方から側面視した状態を示す。図12の流路構造は、3箇所の曲折部を有している。なお、図12に示す大きさや形状はシミュレーションの一例として採用したものであり、本発明の流路構造のサイズ等について限定するものではない。
図14に示す流路構造のモデルについてもコンピュータ解析を行なった。図14の流路構造は、流路の接続部分が、Y方向とX軸方向にそれぞれ0.1mmの余長(つきだし長)を有して接合している(図14の矢印参照)。これ以外は、図12と同様の構造である。
その結果、実施例3でも、3箇所の曲折部を経由したサンプル流は、3次元的にみても均一な流速分布を示し、かつ流路内の中心部分に集束して流れていることが示された。従って、一定の貼り合わせ誤差が生じた場合であっても、良好なサンプル流形状を確保できることが示唆された。
更に、流路構造の曲折部の構造について検討すべく、曲折部の連結構造について比較実験を行った。図15に示す種々の連結構造(1)〜(4)において、今までと同様の条件で流体シミュレーションを行い、これらの連結構造が及ぼす流体への影響を検討した。
連結構造2は、実施例3でシミュレーションした流路構造の曲折部分である。
連結構造3は、流路の接続部分が、Y方向とX軸方向にそれぞれ0.1mmの余長(つきだし長)を有して接合している。即ち、流路が屈曲していく方向と逆方向に所定の余長を設けている。これ以外は、実施例3のモデルと同様の構造である。
連結構造4は、流路の接続部分について、流路を屈曲させる間にH=0.6mmの中継路を設けている。これ以外は、実施例3のモデルと同様の構造である。
これらのシミュレーション結果を図15に示し、その比較結果を表1に示す。
11,21,31 第1導入部
12,22,32 排出部
13,17,23,24,28,29,33,36 第2導入部
14,15,16,25,26,27,34,35 曲折部
Claims (7)
- 試料を導入する第1の導入部と、
前記試料を挟み込むための流体を導入する第2の導入部と、
前記試料を排出する排出部と、を少なくとも備えた流路構造であり、
前記試料の導入方向をX方向とすると、
Y軸周りに略90度に流路が曲折する曲折部と、
X軸周りに略90度に流路が曲折する曲折部と、を少なくとも備える流路構造。 - 更に、Z軸周りに略90度に流路が曲折する曲折部を、流路中に少なくとも備えることを特徴とする請求項1記載の流路構造。
- 前記試料の搬送方向に対して前記流路を断面視した形状は、略同じ形状で維持されていることを特徴とする請求項1記載の流路構造。
- 請求項1記載の流路構造を備えた流路基板。
- 前記試料を流体で挟み込んで流路中を搬送させる際に、順不同で、下記(1)工程と、(2)工程と、を少なくとも行う流体制御方法。
(1)前記試料の導入方向をX方向とすると、前記試料をY軸周りに略90度曲折させる工程、
(2)前記試料をX軸周りに略90度曲折させる工程。 - 前記試料又は前記流体の流体条件を制御することで、前記流路中における前記試料の位置制御を行なうことを特徴とする請求項5記載の流体制御方法。
- 前記試料は細胞及び/又はビーズを含み、前記細胞及び/又はビーズの前記流路中における位置情報を、前記流路中の所定位置において検出し、該位置情報に基づいて前記試料の位置制御を行なうことを特徴とする請求項6記載の流体制御方法。
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007287337A JP4509167B2 (ja) | 2007-11-05 | 2007-11-05 | 流路構造、これを備えた流路基板及び流体制御方法 |
US12/264,881 US20090114285A1 (en) | 2007-11-05 | 2008-11-04 | Flow channel structure, flow channel board having the same, and fluid control method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007287337A JP4509167B2 (ja) | 2007-11-05 | 2007-11-05 | 流路構造、これを備えた流路基板及び流体制御方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2009115542A true JP2009115542A (ja) | 2009-05-28 |
JP4509167B2 JP4509167B2 (ja) | 2010-07-21 |
Family
ID=40586914
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007287337A Expired - Fee Related JP4509167B2 (ja) | 2007-11-05 | 2007-11-05 | 流路構造、これを備えた流路基板及び流体制御方法 |
Country Status (2)
Country | Link |
---|---|
US (1) | US20090114285A1 (ja) |
JP (1) | JP4509167B2 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009162643A (ja) * | 2008-01-08 | 2009-07-23 | Sony Corp | 微小粒子の送流方法及び分析方法、並びに微小粒子分析用基板 |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11243494B2 (en) | 2002-07-31 | 2022-02-08 | Abs Global, Inc. | Multiple laminar flow-based particle and cellular separation with laser steering |
WO2010009247A2 (en) * | 2008-07-18 | 2010-01-21 | 3M Innovative Properties Company | Y-cross mixers and fluid systems including the same |
USD869308S1 (en) | 2010-04-29 | 2019-12-10 | Sony Corporation | Micro flow channel chip |
USD673287S1 (en) | 2010-11-24 | 2012-12-25 | Sony Corporation | Micro flow channel chip |
USD673286S1 (en) | 2010-04-29 | 2012-12-25 | Sony Corporation | Micro flow channel chip |
US10908066B2 (en) | 2010-11-16 | 2021-02-02 | 1087 Systems, Inc. | Use of vibrational spectroscopy for microfluidic liquid measurement |
JP5547120B2 (ja) * | 2011-03-18 | 2014-07-09 | 株式会社神戸製鋼所 | 流路構造体、流体の混合方法、抽出方法及び反応方法 |
WO2012149185A2 (en) * | 2011-04-28 | 2012-11-01 | The Government Of The United States Of America As Represented By The Secretary Of The Navy | Method of changing fluid flow by using an optical beam |
US8961904B2 (en) | 2013-07-16 | 2015-02-24 | Premium Genetics (Uk) Ltd. | Microfluidic chip |
US11796449B2 (en) | 2013-10-30 | 2023-10-24 | Abs Global, Inc. | Microfluidic system and method with focused energy apparatus |
CA2929275C (en) | 2013-10-30 | 2022-03-08 | Premium Genetics (Uk) Ltd. | Microfluidic system and method with focused energy apparatus |
US20170181979A1 (en) * | 2014-03-31 | 2017-06-29 | Shimadzu Corporation | Process for producing nanoparticles |
US10180388B2 (en) | 2015-02-19 | 2019-01-15 | 1087 Systems, Inc. | Scanning infrared measurement system |
US10035887B2 (en) * | 2015-08-19 | 2018-07-31 | Shimadzu Corporation | Manufacturing method for nanoparticle |
CN108603827A (zh) * | 2015-09-11 | 2018-09-28 | 莱布尼茨光电技术研究所 | 用于个体化的患者血液分析的装置 |
DE102015115343B4 (de) * | 2015-09-11 | 2017-10-26 | Leibniz-Institut für Photonische Technologien e. V. | Anordnung und Verfahren für die Fluidrotation |
BR112020023607A2 (pt) | 2018-05-23 | 2021-02-17 | Abs Global, Inc. | sistemas e métodos para focalização de partículas em microcanais |
CN117413819A (zh) | 2019-04-18 | 2024-01-19 | 艾步思国际有限责任公司 | 用于连续添加冷冻保护剂的***和工艺 |
US11628439B2 (en) | 2020-01-13 | 2023-04-18 | Abs Global, Inc. | Single-sheath microfluidic chip |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004509335A (ja) * | 2000-09-18 | 2004-03-25 | ユニバーシティ オブ ワシントン | 複数のフローストリーム間の流体界面の回転操作のための微小流体デバイス |
JP2004167607A (ja) * | 2002-11-15 | 2004-06-17 | Tama Tlo Kk | マイクロ流体素子とその製造方法 |
JP2007148981A (ja) * | 2005-11-30 | 2007-06-14 | Univ Waseda | 微粒子分別マイクロシステムおよび微粒子分別方法 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999060397A1 (en) * | 1998-05-18 | 1999-11-25 | University Of Washington | Liquid analysis cartridge |
EP1125105A2 (en) * | 1998-11-05 | 2001-08-22 | ChemoMetec A/S | A method for the assessment of particles and a system and a device for use in the method |
US6561208B1 (en) * | 2000-04-14 | 2003-05-13 | Nanostream, Inc. | Fluidic impedances in microfluidic system |
US6645432B1 (en) * | 2000-05-25 | 2003-11-11 | President & Fellows Of Harvard College | Microfluidic systems including three-dimensionally arrayed channel networks |
US6890093B2 (en) * | 2000-08-07 | 2005-05-10 | Nanostream, Inc. | Multi-stream microfludic mixers |
US7312085B2 (en) * | 2002-04-01 | 2007-12-25 | Fluidigm Corporation | Microfluidic particle-analysis systems |
AU2003224817B2 (en) * | 2002-04-01 | 2008-11-06 | Fluidigm Corporation | Microfluidic particle-analysis systems |
JP4341372B2 (ja) * | 2003-10-30 | 2009-10-07 | コニカミノルタホールディングス株式会社 | 液体の混合方法および混合装置ならびに混合システム |
US20060270021A1 (en) * | 2004-06-07 | 2006-11-30 | Shuichi Takayama | Integrated microfluidic sperm isolation and insemination device |
US7445027B2 (en) * | 2006-03-14 | 2008-11-04 | The Board Of Trustees Of The University Of Illinois | Multilayer microfluidic-nanofluidic device |
US20090034359A1 (en) * | 2007-04-04 | 2009-02-05 | The Regents Of The University Of California | Stopped flow, quenched flow and continuous flow reaction method and apparatus |
US8120770B2 (en) * | 2007-09-10 | 2012-02-21 | The Penn State Research Foundation | Three-dimensional (3D) hydrodynamic focusing using a microfluidic device |
-
2007
- 2007-11-05 JP JP2007287337A patent/JP4509167B2/ja not_active Expired - Fee Related
-
2008
- 2008-11-04 US US12/264,881 patent/US20090114285A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004509335A (ja) * | 2000-09-18 | 2004-03-25 | ユニバーシティ オブ ワシントン | 複数のフローストリーム間の流体界面の回転操作のための微小流体デバイス |
JP2004167607A (ja) * | 2002-11-15 | 2004-06-17 | Tama Tlo Kk | マイクロ流体素子とその製造方法 |
JP2007148981A (ja) * | 2005-11-30 | 2007-06-14 | Univ Waseda | 微粒子分別マイクロシステムおよび微粒子分別方法 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009162643A (ja) * | 2008-01-08 | 2009-07-23 | Sony Corp | 微小粒子の送流方法及び分析方法、並びに微小粒子分析用基板 |
Also Published As
Publication number | Publication date |
---|---|
JP4509167B2 (ja) | 2010-07-21 |
US20090114285A1 (en) | 2009-05-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4509167B2 (ja) | 流路構造、これを備えた流路基板及び流体制御方法 | |
US8941826B2 (en) | Three-dimensional (3D) hydrodynamic focusing using a microfluidic device | |
CN103191791B (zh) | 生物微粒高通量分选和计数检测的集成芯片***及应用 | |
Shrirao et al. | Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification | |
Grenvall et al. | Two-dimensional acoustic particle focusing enables sheathless chip Coulter counter with planar electrode configuration | |
Barat et al. | Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer | |
JP6396911B2 (ja) | 粒子を選別するためのシステム、装置、および、方法 | |
Chiu et al. | Universally applicable three-dimensional hydrodynamic microfluidic flow focusing | |
JP4661942B2 (ja) | マイクロチップとその流路構造 | |
CN102087197B (zh) | 全功能血液分析仪器中库尔特微孔的共轴照明方法及其分析仪器 | |
JP2011145185A (ja) | 流路構造、マイクロチップ及び送流方法 | |
JP2009109197A (ja) | 微小粒子の測定方法 | |
KR20130045236A (ko) | 마이크로칩 및 미립자 분석 장치 | |
CN104111219A (zh) | 粒子分析装置及其制造方法 | |
Zhang et al. | Design of a single-layer microchannel for continuous sheathless single-stream particle inertial focusing | |
WO2016009467A1 (ja) | マルチチャンネル分析装置 | |
WO2014132876A1 (ja) | マイクロチップを用いた光分析方法および光分析装置、ならびに光分析装置用マイクロチップおよび光分析用処理装置 | |
Ota et al. | Enhancement in acoustic focusing of micro and nanoparticles by thinning a microfluidic device | |
JP2003302330A (ja) | 平板状フローセル装置 | |
JP5157629B2 (ja) | 流路基板 | |
Marcali et al. | Impedimetric detection and lumped element modelling of a hemagglutination assay in microdroplets | |
WO2014038399A1 (ja) | 測定用器具および測定装置 | |
JP5316530B2 (ja) | マイクロチップとその流路構造 | |
JP2012098246A (ja) | 表面プラズモン共鳴測定用マイクロチップ及び表面プラズモン共鳴測定装置 | |
JP2009174995A (ja) | 流路構造、流路基板並びに流体の制御方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20090730 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20090811 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20091104 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20091225 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20100126 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100326 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20100414 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130514 Year of fee payment: 3 |
|
R151 | Written notification of patent or utility model registration |
Ref document number: 4509167 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R151 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20100427 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130514 Year of fee payment: 3 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |