FR2844523B1 - METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER - Google Patents
METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATERInfo
- Publication number
- FR2844523B1 FR2844523B1 FR0306905A FR0306905A FR2844523B1 FR 2844523 B1 FR2844523 B1 FR 2844523B1 FR 0306905 A FR0306905 A FR 0306905A FR 0306905 A FR0306905 A FR 0306905A FR 2844523 B1 FR2844523 B1 FR 2844523B1
- Authority
- FR
- France
- Prior art keywords
- detection
- water
- complex mixture
- identification
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un procédé pour la détection, l'identification et, le cas échéant, la quantification des bactéries contenues dans un mélange complexe ou dans de l'eau, ledit procédé comprenant une étape d'amplifications multiples, simultanées mais séparées dans l'espace, de séquences d'acides nucléiques cibles spécifiques et exclusives. La présente invention a en outre pour objets des couples d'amorces pour amplification spécifiques et exclusifs, utiles à la mise en oeuvre dudit procédé, ainsi que des sondes nucléotidiques pour l'amplification en temps réel ou la détection de séquences d'acides nucléiques spécifiques et exclusives des bactéries contenues dans un mélange complexe ou dans de l'eau. L'invention concerne enfin des outils de biologie moléculaire, à savoir un kit d'analyse et des micro-réseaux ou puces à acides nucléiques pour la détection, l'identification et, le cas échéant, la quantification des bactéries contenues dans un mélange complexe ou dans de l'eau.The invention relates to a method for the detection, identification and, where appropriate, quantification of bacteria contained in a complex mixture or in water, said method comprising a step of multiple amplifications, simultaneous but separate in the space, specific and exclusive target nucleic acid sequences. The present invention also relates to specific and exclusive pairs of primers for amplification, useful for the implementation of said method, as well as nucleotide probes for the amplification in real time or the detection of specific nucleic acid sequences. and exclusive of bacteria contained in a complex mixture or in water. The invention finally relates to molecular biology tools, namely an analysis kit and microarrays or nucleic acid chips for the detection, identification and, where appropriate, quantification of bacteria contained in a complex mixture. or in water.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0306905A FR2844523B1 (en) | 2002-09-13 | 2003-06-06 | METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER |
AU2003282157A AU2003282157A1 (en) | 2002-09-13 | 2003-09-12 | Method and nucleotide sequences for the detection and identification of micro-organisms in a complex mixture or water |
PCT/FR2003/002709 WO2004024944A2 (en) | 2002-09-13 | 2003-09-12 | Method and nucleotide sequences for the detection and identification of micro-organisms in a complex mixture or water |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0211400A FR2844522A1 (en) | 2002-09-13 | 2002-09-13 | Multiplex detection and identification of bacteria in complex mixtures, useful for testing food and environmental samples, by simultaneous, but spatially resolved amplification |
FR0306905A FR2844523B1 (en) | 2002-09-13 | 2003-06-06 | METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2844523A1 FR2844523A1 (en) | 2004-03-19 |
FR2844523B1 true FR2844523B1 (en) | 2011-01-14 |
Family
ID=31947976
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR0306905A Expired - Lifetime FR2844523B1 (en) | 2002-09-13 | 2003-06-06 | METHOD AND NUCLEOTIDE SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF MICROORGANISMS IN A COMPLEX MIXTURE OR IN WATER |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2003282157A1 (en) |
FR (1) | FR2844523B1 (en) |
WO (1) | WO2004024944A2 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BRPI0710889A2 (en) * | 2006-04-24 | 2011-08-16 | Sigma Alimentos Sa De Cv | method for multiple and simultaneous detection and quantification of pathogens by real time polymerase chain reaction |
EP1862557B1 (en) * | 2006-05-29 | 2011-05-18 | Bio-Rad Pasteur | Real-time multiplex detection of three bacterial species responsible for sexually-transmitted diseases |
US9175353B2 (en) | 2008-11-14 | 2015-11-03 | Gen-Probe Incorporated | Compositions, kits and methods for detection of campylobacter nucleic acid |
FR2970264B1 (en) | 2011-01-12 | 2014-09-05 | Ct Scient Tech Batiment Cstb | METHOD AND FRAGMENTS OF NUCLEOTIDES FOR DETECTION AND / OR QUANTIFICATION OF LEGIONELLA HOSTS, KIT THEREFOR |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2812306B1 (en) * | 2000-07-28 | 2005-01-14 | Gabriel Festoc | POLYMERSIS CHAIN AMPLIFICATION SYSTEM OF TARGET NUCLEIC SEQUENCES |
-
2003
- 2003-06-06 FR FR0306905A patent/FR2844523B1/en not_active Expired - Lifetime
- 2003-09-12 WO PCT/FR2003/002709 patent/WO2004024944A2/en not_active Application Discontinuation
- 2003-09-12 AU AU2003282157A patent/AU2003282157A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
FR2844523A1 (en) | 2004-03-19 |
AU2003282157A1 (en) | 2004-04-30 |
WO2004024944A3 (en) | 2004-09-16 |
WO2004024944A2 (en) | 2004-03-25 |
AU2003282157A8 (en) | 2004-04-30 |
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Legal Events
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PLFP | Fee payment |
Year of fee payment: 14 |
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PLFP | Fee payment |
Year of fee payment: 15 |