ES2674480T3 - Método para preparar rebaudiósido A a partir de esteviósido - Google Patents
Método para preparar rebaudiósido A a partir de esteviósido Download PDFInfo
- Publication number
- ES2674480T3 ES2674480T3 ES13876125.9T ES13876125T ES2674480T3 ES 2674480 T3 ES2674480 T3 ES 2674480T3 ES 13876125 T ES13876125 T ES 13876125T ES 2674480 T3 ES2674480 T3 ES 2674480T3
- Authority
- ES
- Spain
- Prior art keywords
- stevioside
- rebaudioside
- preparing rebaudioside
- preparing
- nucleotide diphosphate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1062—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01013—Sucrose synthase (2.4.1.13)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Abstract
Un método para preparar rebaudiósido A, que comprende: (1) hacer reaccionar sacarosa y nucleótido difosfato en presencia de sacarosa sintasa para preparar nucleótido difosfato al que se ha unido la glucosa; y (2) hacer reaccionar el nucleótido difosfato al cual se ha unido glucosa con esteviósido en presencia de glicosiltransferasa para preparar rebaudiósido A.
Description
11
<210> 3
<211> 808
<212> PRT
<213> Sacarosa sintasa
<400> 3
13
15
18
Claims (1)
-
imagen1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020130022176A KR101404728B1 (ko) | 2013-02-28 | 2013-02-28 | 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법 |
KR20130022176 | 2013-02-28 | ||
PCT/KR2013/011330 WO2014133248A1 (ko) | 2013-02-28 | 2013-12-09 | 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2674480T3 true ES2674480T3 (es) | 2018-07-02 |
Family
ID=51132132
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES13876125.9T Active ES2674480T3 (es) | 2013-02-28 | 2013-12-09 | Método para preparar rebaudiósido A a partir de esteviósido |
Country Status (7)
Country | Link |
---|---|
US (1) | US10472660B2 (es) |
EP (1) | EP2963122B1 (es) |
JP (2) | JP6147370B2 (es) |
KR (1) | KR101404728B1 (es) |
CN (1) | CN105164270B8 (es) |
ES (1) | ES2674480T3 (es) |
WO (1) | WO2014133248A1 (es) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9522929B2 (en) | 2014-05-05 | 2016-12-20 | Conagen Inc. | Non-caloric sweetener |
EP3862426A3 (en) | 2015-03-16 | 2021-11-17 | DSM IP Assets B.V. | Udp-glycosyltransferases |
WO2016168413A1 (en) * | 2015-04-14 | 2016-10-20 | Conagen Inc. | Production of non-caloric sweeteners using engineered whole-cell catalysts |
US20170266524A1 (en) * | 2016-03-18 | 2017-09-21 | James C. Van Loon, III | Method of using lacrosse mesh |
WO2017207484A1 (en) | 2016-05-31 | 2017-12-07 | Universiteit Gent | Mutant sucrose synthases and their uses |
MX2018015517A (es) * | 2016-06-15 | 2019-03-18 | Codexis Inc | Glicosilacion enzimatica de glucosidos de esteviol y otros compuestos con glucosa-1-fosfato. |
CA3027189A1 (en) | 2016-06-15 | 2017-12-21 | Codexis, Inc. | Engineered beta-glucosidases and glucosylation methods |
MY194280A (en) | 2016-08-12 | 2022-11-25 | Amyris Inc | Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides |
US20180223264A1 (en) | 2017-02-03 | 2018-08-09 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
CN108315394A (zh) * | 2018-04-23 | 2018-07-24 | 沈阳师范大学 | 甜高粱蔗糖合成酶基因的表达检测方法及扩增引物 |
WO2019161634A1 (zh) * | 2018-07-19 | 2019-08-29 | 邦泰生物工程(深圳)有限公司 | 莱苞迪甙a的制备方法、莱苞迪甙a制备用酶及应用 |
CN111344399B (zh) * | 2018-11-16 | 2023-03-03 | 邦泰生物工程(深圳)有限公司 | 一种udp-葡萄糖基转移酶突变体、其应用及其制备莱鲍迪苷d的方法 |
KR102303415B1 (ko) * | 2019-06-14 | 2021-09-23 | 씨제이제일제당 (주) | 감미 소재 조성물 및 이의 제조 방법 |
CN111621456A (zh) * | 2020-05-25 | 2020-09-04 | 安徽金禾实业股份有限公司 | 一种液态发酵制备莱鲍迪苷a的方法 |
CN111593062A (zh) * | 2020-05-25 | 2020-08-28 | 安徽金禾实业股份有限公司 | 一种利用枯草芽孢杆菌发酵催化制备莱鲍迪苷a的方法 |
TW202342764A (zh) | 2021-10-19 | 2023-11-01 | 南韓商Cj第一製糖股份有限公司 | 用於製備甜菊糖雙苷d及甜菊糖雙苷m的方法 |
KR102628374B1 (ko) | 2021-10-19 | 2024-01-24 | 씨제이제일제당 주식회사 | 신규한 당전이효소 및 이의 용도 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4219571A (en) * | 1978-06-15 | 1980-08-26 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Process for producing a sweetener |
JPS58149697A (ja) * | 1982-02-27 | 1983-09-06 | Dainippon Ink & Chem Inc | β−1,3グリコシルステビオシドの製造方法 |
JPH11290075A (ja) | 1998-02-26 | 1999-10-26 | Univ Kyoto | 組み換えミュータントシュクロース合成酵素によるシュクロースからのセルロースの合成 |
JP2000245279A (ja) | 1999-03-01 | 2000-09-12 | Mitsui Chemicals Inc | 植物の耐冷性の増強方法 |
US6469947B2 (en) | 1999-06-29 | 2002-10-22 | Hyundai Electronics Co., Ltd. | Semiconductor memory device having regions with independent word lines alternately selected for refresh operation |
KR20040026747A (ko) | 2002-09-26 | 2004-04-01 | 바이오스펙트럼 주식회사 | 미생물을 이용한 리보디오사이드 에이의 제조방법 |
US7884265B2 (en) | 2005-03-04 | 2011-02-08 | Toyoshige Morita | High Rebaudioside-A plant |
FR2887253A1 (fr) | 2005-06-20 | 2006-12-22 | Phosphoenix Sarl | Nouvelles arylphosphines p chirales ortho-fonctionnalisees et derives: leur preparation et utilisation en catalyse asymetrique |
JP2007330112A (ja) | 2006-06-12 | 2007-12-27 | Sanei Gen Ffi Inc | フェノール性化合物のグルコース配糖体の製造方法 |
CA2676220C (en) * | 2007-01-22 | 2016-04-05 | Cargill, Incorporated | Method of producing purified rebaudioside a compositions using solvent/antisolvent crystallization |
US9005444B2 (en) * | 2008-05-13 | 2015-04-14 | Cargill, Incorporated | Separation of rebaudioside A from stevia glycosides using chromatography |
KR101598935B1 (ko) | 2010-04-16 | 2016-03-03 | 씨제이제일제당(주) | 레바우디오사이드 a의 생산 공정에서 발생하는 부산물을 재활용하여 고수득율의 레바우디오사이드 a를 제조하는 방법 |
SG186127A1 (en) | 2010-06-02 | 2013-01-30 | Evolva Nutrition Inc | Recombinant production of steviol glycosides |
CA3128532A1 (en) * | 2011-08-08 | 2013-02-14 | Evolva Sa | Recombinant production of steviol glycosides |
CN102766667B (zh) * | 2012-08-14 | 2014-06-11 | 成都南诺格生物科技有限责任公司 | 甜菊苷转化为莱鲍迪苷e的方法 |
-
2013
- 2013-02-28 KR KR1020130022176A patent/KR101404728B1/ko active IP Right Grant
- 2013-12-09 ES ES13876125.9T patent/ES2674480T3/es active Active
- 2013-12-09 EP EP13876125.9A patent/EP2963122B1/en active Active
- 2013-12-09 CN CN201380073982.0A patent/CN105164270B8/zh active Active
- 2013-12-09 US US14/770,962 patent/US10472660B2/en active Active
- 2013-12-09 WO PCT/KR2013/011330 patent/WO2014133248A1/ko active Application Filing
- 2013-12-09 JP JP2015560087A patent/JP6147370B2/ja active Active
-
2017
- 2017-03-10 JP JP2017046085A patent/JP2017148050A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
JP6147370B2 (ja) | 2017-06-21 |
CN105164270B (zh) | 2019-07-09 |
JP2017148050A (ja) | 2017-08-31 |
CN105164270A (zh) | 2015-12-16 |
US10472660B2 (en) | 2019-11-12 |
WO2014133248A1 (ko) | 2014-09-04 |
EP2963122A1 (en) | 2016-01-06 |
KR101404728B1 (ko) | 2014-06-09 |
EP2963122A4 (en) | 2016-10-12 |
US20160010133A1 (en) | 2016-01-14 |
JP2016508378A (ja) | 2016-03-22 |
EP2963122B1 (en) | 2018-05-30 |
CN105164270B8 (zh) | 2019-12-06 |
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