ES2674480T3 - Método para preparar rebaudiósido A a partir de esteviósido - Google Patents

Método para preparar rebaudiósido A a partir de esteviósido Download PDF

Info

Publication number
ES2674480T3
ES2674480T3 ES13876125.9T ES13876125T ES2674480T3 ES 2674480 T3 ES2674480 T3 ES 2674480T3 ES 13876125 T ES13876125 T ES 13876125T ES 2674480 T3 ES2674480 T3 ES 2674480T3
Authority
ES
Spain
Prior art keywords
stevioside
rebaudioside
preparing rebaudioside
preparing
nucleotide diphosphate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES13876125.9T
Other languages
English (en)
Inventor
Sung Hee Park
Jung Eun Kim
Ran Young Yoon
Young Ho Hong
Seong Bo Kim
Seung Won Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CJ CheilJedang Corp
Original Assignee
CJ CheilJedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CJ CheilJedang Corp filed Critical CJ CheilJedang Corp
Application granted granted Critical
Publication of ES2674480T3 publication Critical patent/ES2674480T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

Un método para preparar rebaudiósido A, que comprende: (1) hacer reaccionar sacarosa y nucleótido difosfato en presencia de sacarosa sintasa para preparar nucleótido difosfato al que se ha unido la glucosa; y (2) hacer reaccionar el nucleótido difosfato al cual se ha unido glucosa con esteviósido en presencia de glicosiltransferasa para preparar rebaudiósido A.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
imagen9
imagen10
11
imagen11
imagen12
<210> 3
<211> 808
<212> PRT
<213> Sacarosa sintasa
<400> 3
13
imagen13
imagen14
15
imagen15
imagen16
imagen17
18

Claims (1)

  1. imagen1
ES13876125.9T 2013-02-28 2013-12-09 Método para preparar rebaudiósido A a partir de esteviósido Active ES2674480T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020130022176A KR101404728B1 (ko) 2013-02-28 2013-02-28 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법
KR20130022176 2013-02-28
PCT/KR2013/011330 WO2014133248A1 (ko) 2013-02-28 2013-12-09 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법

Publications (1)

Publication Number Publication Date
ES2674480T3 true ES2674480T3 (es) 2018-07-02

Family

ID=51132132

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13876125.9T Active ES2674480T3 (es) 2013-02-28 2013-12-09 Método para preparar rebaudiósido A a partir de esteviósido

Country Status (7)

Country Link
US (1) US10472660B2 (es)
EP (1) EP2963122B1 (es)
JP (2) JP6147370B2 (es)
KR (1) KR101404728B1 (es)
CN (1) CN105164270B8 (es)
ES (1) ES2674480T3 (es)
WO (1) WO2014133248A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
EP3862426A3 (en) 2015-03-16 2021-11-17 DSM IP Assets B.V. Udp-glycosyltransferases
WO2016168413A1 (en) * 2015-04-14 2016-10-20 Conagen Inc. Production of non-caloric sweeteners using engineered whole-cell catalysts
US20170266524A1 (en) * 2016-03-18 2017-09-21 James C. Van Loon, III Method of using lacrosse mesh
WO2017207484A1 (en) 2016-05-31 2017-12-07 Universiteit Gent Mutant sucrose synthases and their uses
MX2018015517A (es) * 2016-06-15 2019-03-18 Codexis Inc Glicosilacion enzimatica de glucosidos de esteviol y otros compuestos con glucosa-1-fosfato.
CA3027189A1 (en) 2016-06-15 2017-12-21 Codexis, Inc. Engineered beta-glucosidases and glucosylation methods
MY194280A (en) 2016-08-12 2022-11-25 Amyris Inc Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides
US20180223264A1 (en) 2017-02-03 2018-08-09 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
CN108315394A (zh) * 2018-04-23 2018-07-24 沈阳师范大学 甜高粱蔗糖合成酶基因的表达检测方法及扩增引物
WO2019161634A1 (zh) * 2018-07-19 2019-08-29 邦泰生物工程(深圳)有限公司 莱苞迪甙a的制备方法、莱苞迪甙a制备用酶及应用
CN111344399B (zh) * 2018-11-16 2023-03-03 邦泰生物工程(深圳)有限公司 一种udp-葡萄糖基转移酶突变体、其应用及其制备莱鲍迪苷d的方法
KR102303415B1 (ko) * 2019-06-14 2021-09-23 씨제이제일제당 (주) 감미 소재 조성물 및 이의 제조 방법
CN111621456A (zh) * 2020-05-25 2020-09-04 安徽金禾实业股份有限公司 一种液态发酵制备莱鲍迪苷a的方法
CN111593062A (zh) * 2020-05-25 2020-08-28 安徽金禾实业股份有限公司 一种利用枯草芽孢杆菌发酵催化制备莱鲍迪苷a的方法
TW202342764A (zh) 2021-10-19 2023-11-01 南韓商Ｃｊ第一製糖股份有限公司 用於製備甜菊糖雙苷d及甜菊糖雙苷m的方法
KR102628374B1 (ko) 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4219571A (en) * 1978-06-15 1980-08-26 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Process for producing a sweetener
JPS58149697A (ja) * 1982-02-27 1983-09-06 Dainippon Ink & Chem Inc β−１，３グリコシルステビオシドの製造方法
JPH11290075A (ja) 1998-02-26 1999-10-26 Univ Kyoto 組み換えミュータントシュクロース合成酵素によるシュクロースからのセルロースの合成
JP2000245279A (ja) 1999-03-01 2000-09-12 Mitsui Chemicals Inc 植物の耐冷性の増強方法
US6469947B2 (en) 1999-06-29 2002-10-22 Hyundai Electronics Co., Ltd. Semiconductor memory device having regions with independent word lines alternately selected for refresh operation
KR20040026747A (ko) 2002-09-26 2004-04-01 바이오스펙트럼 주식회사 미생물을 이용한 리보디오사이드 에이의 제조방법
US7884265B2 (en) 2005-03-04 2011-02-08 Toyoshige Morita High Rebaudioside-A plant
FR2887253A1 (fr) 2005-06-20 2006-12-22 Phosphoenix Sarl Nouvelles arylphosphines p chirales ortho-fonctionnalisees et derives: leur preparation et utilisation en catalyse asymetrique
JP2007330112A (ja) 2006-06-12 2007-12-27 Sanei Gen Ffi Inc フェノール性化合物のグルコース配糖体の製造方法
CA2676220C (en) * 2007-01-22 2016-04-05 Cargill, Incorporated Method of producing purified rebaudioside a compositions using solvent/antisolvent crystallization
US9005444B2 (en) * 2008-05-13 2015-04-14 Cargill, Incorporated Separation of rebaudioside A from stevia glycosides using chromatography
KR101598935B1 (ko) 2010-04-16 2016-03-03 씨제이제일제당(주) 레바우디오사이드 ａ의 생산 공정에서 발생하는 부산물을 재활용하여 고수득율의 레바우디오사이드 ａ를 제조하는 방법
SG186127A1 (en) 2010-06-02 2013-01-30 Evolva Nutrition Inc Recombinant production of steviol glycosides
CA3128532A1 (en) * 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
CN102766667B (zh) * 2012-08-14 2014-06-11 成都南诺格生物科技有限责任公司 甜菊苷转化为莱鲍迪苷e的方法

Also Published As

Publication number Publication date
JP6147370B2 (ja) 2017-06-21
CN105164270B (zh) 2019-07-09
JP2017148050A (ja) 2017-08-31
CN105164270A (zh) 2015-12-16
US10472660B2 (en) 2019-11-12
WO2014133248A1 (ko) 2014-09-04
EP2963122A1 (en) 2016-01-06
KR101404728B1 (ko) 2014-06-09
EP2963122A4 (en) 2016-10-12
US20160010133A1 (en) 2016-01-14
JP2016508378A (ja) 2016-03-22
EP2963122B1 (en) 2018-05-30
CN105164270B8 (zh) 2019-12-06

Similar Documents

Publication Publication Date Title
ES2674480T3 (es) Método para preparar rebaudiósido A a partir de esteviósido
AR100277A1 (es) Edulcorante no calórico
BR112016012883A2 (pt) Métodos e composições para aprimorar o rendimento de milho
CL2016001366A1 (es) Presentación de antígeno de cáncer mejorado a células que presentan antígeno por la descomposición eléctrica de membrana por radiofrecuencia (rf-emb) como un mecanismo adyuvante para la inmunoterapia.
EA202092987A3 (ru) Липид, содержащий докозапентаеновую кислоту
WO2015089511A3 (en) Modified nucleic acid molecules and uses thereof
BR112014009066B8 (pt) molécula de ácido nucleico, método para a sintetização de uma molécula de dna de triciclo-fosforotioato e composição farmacêutica
LT2940030T (lt) P1,p4-di (uridino 5`-) tetrafosfato gamybos būdas
BR112012005591A2 (pt) Geração de sementes oleaginosas produzindo alto poliidroxibutirato.
WO2014153188A3 (en) High efficiency, small volume nucleic acid synthesis
GB201214580D0 (en) Benzocyanine compounds
AR079704A1 (es) Anticuerpos anti-bv8 y sus usos
ZA201509055B (en) 4&#39;-azido, 3&#39;-deoxy-3&#39;-fluoro substituted nucleoside derivatives
CO2017000010A2 (es) Proceso para la preparacion de ácidos 3-hidroxipicolínicos
GB2495884A (en) A method of increasing the effect of an activated-potentiated form of an antibody
IT1397886B1 (it) Procedimento per la produzione di componenti di carburanti per autotrazione.
EA201690245A1 (ru) Оптимизированный способ получения композиции, содержащей изомальтулозу
WO2015063549A9 (en) A method of preparing fermentable sugars from biomass resources
WO2012151142A3 (en) Flash and glow 1,2-dioxetanes
PH12014501367A1 (en) Processes for making magnolol and derivatives thereof
BR112014007818A2 (pt) co-cultura sintrófica de micro-organismos anaeróbicos para a produção de n-butanol a partir de gás de síntese
BR112014015031A2 (pt) processo para fabricação de análogos de magnolol
ME02217B (me) Postupak za enzimsku sintezu (7S)- 3,4-dimetoksibiciklo[4.2.0]okta-1,3,5-trien-7-karboksilne kiseline i upotreba za sintezu ivabradina i njegovih soli
ES2405298B1 (es) Procedimiento de fraccionamiento de biomasa lignocelulósica.
IT1396107B1 (it) Supporto ambidestro per la realizzazione di infissi dotati di alette spioventi.