ES2422291T3 - Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas - Google Patents

Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas

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ES2422291T3
ES2422291T3 ES08845549T ES08845549T ES2422291T3 ES 2422291 T3 ES2422291 T3 ES 2422291T3 ES 08845549 T ES08845549 T ES 08845549T ES 08845549 T ES08845549 T ES 08845549T ES 2422291 T3 ES2422291 T3 ES 2422291T3
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semisite
recognition
seq
recognition sequences
laglidadg
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James Jefferson Smith
Derek Jantz
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Precision Biosciences Inc
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Precision Biosciences Inc
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Abstract

Una meganucleasa monocatenaria recombinante que comprende: una primera subunidad LAGLIDADG que comprende una secuencia polipeptídica que tiene al menos un 85% de identidad de secuencia con los restos 9-151 de una meganucleasa I-CreI de tipo salvaje de SEC IDNº: 1 y que tiene un primer semisitio de reconocimiento; una segunda subunidad LAGLIDADG que comprende un polipéptido que tiene al menos un 85 % deidentidad de secuencia con los restos 9-151 de una meganucleasa I-CreI de tipo salvaje de SEC ID Nº: 1 yque tiene un segundo semisitio de reconocimiento; en la que dichas primera y segunda subunidades LAGLIDADG están unidas covalentemente con unengarce que consiste en cualquiera de las SEC ID Nº: 77-93 o con un engarce que comprende del extremoN terminal al C terminal un primer bucle, una primera hélice α, una primera vuelta, una segunda hélice α yun segundo bucle, y en la que dichas primera y segunda subunidades LAGLIDADG son capaces de actuar juntas parareconocer y escindir una secuencia de ADN no palindrómica que es un híbrido de dicho primer semisitio dereconocimiento y dicho segundo semisitio de reconocimiento.
ES08845549T 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas Active ES2422291T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US124707P 2007-10-31 2007-10-31
PCT/US2008/082072 WO2009059195A2 (en) 2007-10-31 2008-10-31 Rationally-designed single-chain meganucleases with non-palindromic recognition sequences

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ES2422291T3 true ES2422291T3 (es) 2013-09-10

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ES13165733.0T Active ES2575412T3 (es) 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas
ES08845549T Active ES2422291T3 (es) 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas
ES16163925T Active ES2732735T3 (es) 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas

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US (9) US8445251B2 (es)
EP (4) EP2215223B1 (es)
JP (6) JP5761996B2 (es)
AU (1) AU2008318430A1 (es)
CA (1) CA2704383A1 (es)
DK (3) DK3098309T3 (es)
ES (3) ES2575412T3 (es)
WO (1) WO2009059195A2 (es)

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