ES2422291T3 - Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas - Google Patents
Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicasInfo
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- ES2422291T3 ES2422291T3 ES08845549T ES08845549T ES2422291T3 ES 2422291 T3 ES2422291 T3 ES 2422291T3 ES 08845549 T ES08845549 T ES 08845549T ES 08845549 T ES08845549 T ES 08845549T ES 2422291 T3 ES2422291 T3 ES 2422291T3
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/04—Phosphoric diester hydrolases (3.1.4)
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- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Una meganucleasa monocatenaria recombinante que comprende: una primera subunidad LAGLIDADG que comprende una secuencia polipeptídica que tiene al menos un 85% de identidad de secuencia con los restos 9-151 de una meganucleasa I-CreI de tipo salvaje de SEC IDNº: 1 y que tiene un primer semisitio de reconocimiento; una segunda subunidad LAGLIDADG que comprende un polipéptido que tiene al menos un 85 % deidentidad de secuencia con los restos 9-151 de una meganucleasa I-CreI de tipo salvaje de SEC ID Nº: 1 yque tiene un segundo semisitio de reconocimiento; en la que dichas primera y segunda subunidades LAGLIDADG están unidas covalentemente con unengarce que consiste en cualquiera de las SEC ID Nº: 77-93 o con un engarce que comprende del extremoN terminal al C terminal un primer bucle, una primera hélice α, una primera vuelta, una segunda hélice α yun segundo bucle, y en la que dichas primera y segunda subunidades LAGLIDADG son capaces de actuar juntas parareconocer y escindir una secuencia de ADN no palindrómica que es un híbrido de dicho primer semisitio dereconocimiento y dicho segundo semisitio de reconocimiento.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US124707P | 2007-10-31 | 2007-10-31 | |
PCT/US2008/082072 WO2009059195A2 (en) | 2007-10-31 | 2008-10-31 | Rationally-designed single-chain meganucleases with non-palindromic recognition sequences |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2422291T3 true ES2422291T3 (es) | 2013-09-10 |
Family
ID=40591777
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES13165733.0T Active ES2575412T3 (es) | 2007-10-31 | 2008-10-31 | Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas |
ES08845549T Active ES2422291T3 (es) | 2007-10-31 | 2008-10-31 | Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas |
ES16163925T Active ES2732735T3 (es) | 2007-10-31 | 2008-10-31 | Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES13165733.0T Active ES2575412T3 (es) | 2007-10-31 | 2008-10-31 | Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES16163925T Active ES2732735T3 (es) | 2007-10-31 | 2008-10-31 | Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas |
Country Status (8)
Country | Link |
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US (9) | US8445251B2 (es) |
EP (4) | EP2215223B1 (es) |
JP (6) | JP5761996B2 (es) |
AU (1) | AU2008318430A1 (es) |
CA (1) | CA2704383A1 (es) |
DK (3) | DK3098309T3 (es) |
ES (3) | ES2575412T3 (es) |
WO (1) | WO2009059195A2 (es) |
Families Citing this family (115)
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