ES2273447T3 - Plantas transgenicas resistentes a insectos y procedimientos para mejorar la actividad frente a insectos. - Google Patents

Plantas transgenicas resistentes a insectos y procedimientos para mejorar la actividad frente a insectos. Download PDF

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Publication number
ES2273447T3
ES2273447T3 ES98964762T ES98964762T ES2273447T3 ES 2273447 T3 ES2273447 T3 ES 2273447T3 ES 98964762 T ES98964762 T ES 98964762T ES 98964762 T ES98964762 T ES 98964762T ES 2273447 T3 ES2273447 T3 ES 2273447T3
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ES
Spain
Prior art keywords
replaced
asparagine
arginine
valine
proline
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98964762T
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English (en)
Inventor
Leigh English
Susan M Brussock
Thomas M Malvar
James W Bryson
Caroline A Kulesza
Frederick S Walters
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Monsanto Technology LLC
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Monsanto Technology LLC
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Publication date
Priority claimed from US08/993,170 external-priority patent/US6063597A/en
Priority claimed from US08/996,441 external-priority patent/US6023013A/en
Priority claimed from US08/993,722 external-priority patent/US6060594A/en
Priority claimed from US08/993,775 external-priority patent/US6077824A/en
Application filed by Monsanto Technology LLC filed Critical Monsanto Technology LLC
Application granted granted Critical
Publication of ES2273447T3 publication Critical patent/ES2273447T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Un polipéptido Cry3Bb de B. thuringiensis modificado que comprende de uno a cinco cambios de aminoácidos en la secuencia principal del polipéptido Cry3Bb de la SEC DE ID Nº: 98, en la que dichos cambios de aminoácidos se seleccionan entre: Asp103 se sustituye por ácido glutámico; Thr154 se sustituye por glicina o fenilalanina; Pro155 se sustituye por histidina; Leu156 se sustituye por histidina; Leu158 se sustituye por arginina; Ser160 se sustituye por asparagina; Lys161 se sustituye por prolina; Arg162 se sustituye por histidina; Asp165 se sustituye por glicina; Lys189 se sustituye por glicina; Ser223 se sustituye por prolina; Tyr230 se sustituye por leucina o serina; His231 se sustituye por arginina, asparagina, serina o treonina; Thr241 se sustituye por serina; Tyr287 se sustituye por fenilalanina; Asp288 se sustituye por asparagina; Ile289 se sustituye por treonina o valina; Arg290 se sustituye por asparagina, leucina o valina; Leu291 se sustituye por arginina; Tyr292 se sustituye por fenilalanina; Ser293 se sustituye por arginina o prolina; Phe305 se sustituye por serina; Ser311 se sustituye por alanina, isoleucina, leucina, o treonina; Leu312 se sustituye por prolina o valina; Asn313 se sustituye por arginina, histidina, treonina o valina; Thr314 se sustituye por asparagina; Leu315 se sustituye por prolina; Gln316 se sustituye por ácido aspártico, leucina, metionina, o triptófano; Glu317 se sustituye por alanina, asparagina, lisina o valina; Tyr318 se sustituye por cisteína; Gln348 se sustituye por arginina; Val365 se sustituye por alanina; y Ala104 se suprime.
ES98964762T 1997-12-18 1998-12-17 Plantas transgenicas resistentes a insectos y procedimientos para mejorar la actividad frente a insectos. Expired - Lifetime ES2273447T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/993,170 US6063597A (en) 1997-12-18 1997-12-18 Polypeptide compositions toxic to coleopteran insects
US08/996,441 US6023013A (en) 1997-12-18 1997-12-18 Insect-resistant transgenic plants
US08/993,722 US6060594A (en) 1997-12-18 1997-12-18 Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins
US08/993,775 US6077824A (en) 1997-12-18 1997-12-18 Methods for improving the activity of δ-endotoxins against insect pests

Publications (1)

Publication Number Publication Date
ES2273447T3 true ES2273447T3 (es) 2007-05-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES98964762T Expired - Lifetime ES2273447T3 (es) 1997-12-18 1998-12-17 Plantas transgenicas resistentes a insectos y procedimientos para mejorar la actividad frente a insectos.

Country Status (13)

Country Link
EP (3) EP1801220B1 (es)
CN (1) CN100340666C (es)
AR (3) AR014144A1 (es)
AT (3) ATE544857T1 (es)
AU (1) AU2001399A (es)
BR (1) BR9814294B1 (es)
CA (1) CA2314429C (es)
CY (1) CY1106323T1 (es)
DE (1) DE69835538T2 (es)
DK (1) DK1040192T3 (es)
ES (1) ES2273447T3 (es)
PT (1) PT1040192E (es)
WO (1) WO1999031248A1 (es)

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AR064767A2 (es) 2009-04-22
AR023762A2 (es) 2002-09-04
EP1040192A1 (en) 2000-10-04
DE69835538D1 (de) 2006-09-21
BR9814294A (pt) 2001-10-23
EP1801220A2 (en) 2007-06-27
ATE555123T1 (de) 2012-05-15
EP1801220A3 (en) 2007-08-22
EP1040192B1 (en) 2006-08-09
DK1040192T3 (da) 2006-12-18
EP1749834B1 (en) 2012-04-25
ATE544857T1 (de) 2012-02-15
CA2314429C (en) 2010-03-02
DE69835538T2 (de) 2008-01-03
WO1999031248A9 (en) 1999-09-16
CN1286728A (zh) 2001-03-07
BR9814294B1 (pt) 2011-10-18
CY1106323T1 (el) 2011-10-12
EP1749834A1 (en) 2007-02-07
WO1999031248A1 (en) 1999-06-24
CA2314429A1 (en) 1999-06-24
PT1040192E (pt) 2006-12-29
ATE335822T1 (de) 2006-09-15
AR014144A1 (es) 2001-02-07
EP1801220B1 (en) 2012-02-08
AU2001399A (en) 1999-07-05
CN100340666C (zh) 2007-10-03

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