EP2399128A1 - Diagnosegerät - Google Patents
DiagnosegerätInfo
- Publication number
- EP2399128A1 EP2399128A1 EP10704554A EP10704554A EP2399128A1 EP 2399128 A1 EP2399128 A1 EP 2399128A1 EP 10704554 A EP10704554 A EP 10704554A EP 10704554 A EP10704554 A EP 10704554A EP 2399128 A1 EP2399128 A1 EP 2399128A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- electrode
- diagnostic device
- voltage
- nutrient solution
- electrical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48735—Investigating suspensions of cells, e.g. measuring microbe concentration
Definitions
- the invention relates to a diagnostic device.
- Such a diagnostic device is used for example for the detection of Helicobacter pylori.
- a common cause of discomfort in the upper gastrointestinal tract is a bacterial infestation of its organs.
- Helicobacter pylori infestation is believed to be responsible for a whole range of gastric disorders associated with increased gastric acid secretion. These include, for example, the Type B
- Gastritis in about 75% of gastric ulcers and almost all twelve-finger gut ulcers.
- the examination of the hollow organs of the gastrointestinal tract for colonization with bacteria, in particular colonization with Helicobacter pylori is therefore an important part of the diagnosis of gastric diseases.
- Helicobacter pylori is detected via a breath test in which a patient is administered a C-13 masked urea. The C-13 masked CO 2 resulting from the cleavage of urea (CO (NH 2 ) 2 ) into ammonia (NH 3 ) and carbon dioxide (CO 2 ) is detected in the exhaled air.
- CO (NH 2 ) 2 cleavage of urea
- NH 3 ammonia
- CO 2 carbon dioxide
- Other methods of detecting Helicobacter pylori are indicative of typical blood levels, such as pepsinogen or gastrin. However, such methods are complicated and only partially reliable.
- Another test for Helicobacter pylori is the detection of Helicobacter pylori antigen in stool.
- stomach for colonization with Helicobacter pylori Another possibility to examine the stomach for colonization with Helicobacter pylori is the so-called gastroscopy ("gastroscopy").
- gastroscopy During such tant ⁇ monitoring the gastroenterologist takes by means of a biopsy, a Tissue sample (biopsy) from the gastric mucosa, to examine these immediately or at a later date for infection with Helicobacter pylori.
- a well-known examination procedure for the tissue sample is, for example, the Helicobacter urease test (HU test, HUT for short).
- the biopsy is placed in a test medium (measuring solution), which consists of a nutrient solution for this bacterium, urea and an indicator (litmus).
- the bacterium splits the urea (CO (NH2) 2) by urease into ammonia (NH3) and carbon dioxide (CO2). • The ammonia then colors the indicator red. The test result can be seen after a few minutes. The onset of color change from yellow to red is not clearly identifiable under unfavorable conditions.
- An alternative to a gastroscopy performed by means of a flexible endoscope is the use of a so-called endoscopy capsule.
- Such an endoscopy capsule which is also referred to as a capsule endoscope or endocapsule, is designed as a passive endocapsule or as a navigable endocapsule.
- a passive endoscopy capsule moves through the patient's intestine due to peristalsis.
- a navigable endocapsule is known, for example, from the patent with the publication number DE 101 42 253 C1 and from the corresponding patent application with the publication number US 2003/0060702 A1, where it is referred to as "endo-robot” or "endo-robot".
- the endo-robot known from these publications can be navigated in a hollow organ (eg gastrointestinal tract) of a patient by means of a magnetic field which is generated by an external (ie arranged outside the patient) magnet system (coil system).
- An integrated system for position control which includes a position measurement of the endo-robot and an automatic control of the magnetic field or the coil currents, can automatically detect and compensate for changes in the position of the endorobot in the hollow organ of the patient.
- endorobot can be targeted in desired regions of the hollow organ to be navigated.
- This type of capsule endoscopy is therefore also referred to as MGCE (Magnetically Guided Capsule Endoscopy - magnetically guided capsule endoscopy).
- Object of the present invention is to provide a diagnostic device with which a fresh tissue sample can be examined in a very short time for Helicobacter pylori.
- the diagnostic device comprises a first electrode (reference electrode) made of a noble metal, which is not attacked by acid (eg hydrochloric acid, phosphoric acid, sulfuric acid, gastric acid), and a second electrode (measuring electrode) made of silver, wherein the first electrode and the second electrode at least partially immersed in a container which is filled with a nutrient solution (measuring solution) and in which a tissue sample can be introduced, and wherein between the first electrode and the second electrode, an electrical voltage can be applied and in the presence of ammonia between the first electrode and the second electrode is a change in electrical size measurable.
- a first electrode reference electrode
- a noble metal which is not attacked by acid
- a second electrode measuring electrode
- the electrical voltage between the first electrode and the second electrode is equal to zero.
- no current flows between the first electrode and the second electrode.
- the potential that is to say without current, is thus advantageously measured. As a result, there is hardly any ion migration in the acidic nutrient solution.
- the electrical voltage between the first electrode and the second electrode an alternating voltage with a variably predeterminable frequency spectrum.
- the ions migrate to the associated electrodes, the cations (eg ammonium NH 4 + ) to the cathode and the anions (eg chloride Cl " ) to the anode in the diagnostic device according to claim 3, a complete charging of the first electrode (reference electrode) and a complete charging of the second electrode (measuring electrode) reliably prevented because at a sufficiently high frequency, the migration speed of the ions in the acidic nutrient solution (measurement solution) is almost zero.
- the second electrode which according to the invention consists of silver (Ag), cyclically switches between destruction and a structure of the silver chloride layer (AgCl). Both the destruction of the silver chloride layer and its structure can be measured, for example via an impedance measurement and compared cyclically.
- the measurable potential differences and phase differences are characteristic of the presence of a urease activity, which can be concluded with a very high degree of certainty for the presence of Helicobacter pylori.
- the frequency spectrum of the alternating voltage is modulated. This results in increased stability of the AC voltage, which increases the measurement accuracy and reduces the measurement time.
- the electrical voltage between the first electrode and the second electrode is an applicable for a predetermined time DC voltage.
- the predeterminable time for which a voltage can be applied by the user between the first electrode and the second electrode, can be between zero seconds and permanently.
- zer selected electrical voltage here may be zero volts or higher.
- a time of zero seconds or a voltage of zero volts is a passive measurement. In the case of deviating values, an active measurement is available.
- electrical quantities e.g. Potentials, electrical currents or electrical resistances or their changes or variables derived from the electrical quantities (for example electrical conductivity) or their changes are measured.
- the second electrode (measuring electrode) made of silver (Ag) in the diagnostic apparatus according to claim 1 must be etched by hydrochloric acid (HCl). This can - but does not have to be - done for the first time before the delivery of the diagnostic device or the second electrode. However, it is also possible for the user to carry out the first-time HCl etching himself or to apply a corresponding silver chloride layer by means of a suitable electrolytic process. After its HCl etching or after its electrolytic deposition, the second electrode has on its surface a coating of silver chloride (AgCl) and is thus activated for the measurement for the detection of Helicobacter pylori.
- HCl hydrochloric acid
- the diagnostic device enables easy control or simple regulation of the sensor or its first electrode (reference electrode) and / or its second electrode (measuring electrode), e.g. through a baseline correction. Furthermore, after each examination, a reproducible regeneration of the second electrode, i. Elimination of damage caused by ammonia in the silver chloride layer, possible.
- the sensitivity of the sensor or its first electrode and / or its second electrode can be adjusted in a simple manner in the diagnostic device according to the invention. Sensitivity adjustment can be made before and during analysis for Helicobacter pylori.
- platinum (Pt) and gold (Au) come into question.
- the nutrient solution is an acidic nutrient solution, in particular a salt-containing nutrient solution.
- a buffered nutrient solution is particularly preferred.
- urea is added to the acidic nutrient solution.
- a tissue sample taken from the gastrointestinal tract is now introduced into the salt-saponified nutrient solution (pH value similar to that of the stomach), an infestation of the tissue sample with Helicobacter pylori can be detected by the detection of ammonia (NH 3 ).
- Ammonia is produced by the Helicobacter pylori bacteria by a breakdown of urea by urease in order to protect against the acidic environment of the gastrointestinal tract, in particular the high acid concentration in the stomach.
- the second electrode (measuring electrode) made of silver (Ag) in the diagnostic apparatus according to claim 1 must be etched by hydrochloric acid (HCl). After its HCl etching, the second electrode has on its surface a coating of silver chloride (AgCl) and is thus activated for the measurement for the detection of Helicobacter pylori. Activation of the second electrode is based on the following chemical reaction: Ag + HCl ⁇ AgCl + H + + + e "
- ammonia (NH 3 ) under normal circumstances in a hollow organ of the gastrointestinal tract, such as the stomach, due to the following neutralization reaction (formation of an ammonium cation by protonation of ammonia)
- H + hydrogen nucleus
- the salt AgCl (silver chloride) is split by ammonia into the silver-diamine complex [Ag (NH 3 ) 2] + and in chlorine Cl " .
- [Ag (NH 3 ) 2 ] + is excellently soluble in water as a cation
- the diagnostic device according to the invention is either an electrical voltage of zero (claim 2) or there is an electrical alternating voltage with a Alternatively, a DC voltage may be applied between the first electrode and the second electrode for a predeterminable time (claim 4) . In all cases, there is hardly any migration of ions in the nutrient solution (migration speed of the cations and anions is almost zero) zero).
- the electrical quantity measured between the first electrode (reference electrode) and the second electrode (measuring electrode) is logged, displayed and - if desired - transmitted to an evaluation.
- a (automated) comparison of the measured value with predefined values a possible infiltration of the gastric mucosa onto Helicobacter pylori can be reliably displayed.
- the container and the electrodes are first subjected to disinfection with a cleaning solution (for example, ethanol or isopropanol), followed by rinsing with rinsing solution (hydrochloric acid or a mixture of hydrochloric acid and urea).
- a cleaning solution for example, ethanol or isopropanol
- rinsing solution hydrochloric acid or a mixture of hydrochloric acid and urea
- the diagnostic device according to the invention can thus be used again after a refilling of the container with a nutrient solution, preferably with an acidic nutrient solution, in particular with a buffered nutrient solution, and after any necessary recalibration for the detection of Helicobacter pylori.
- a calibration of the diagnostic device can be done for example by a dosage of synthetic ammonia.
- the diagnostic device according to the invention thus allows a very rapid examination of removed tissue samples for Helicobacter pylori.
- Both the first electrode (reference electrode) and the second electrode (measuring electrode) may be formed as separate rod-shaped or planar electrodes which are at least partially immersed in the nutrient solution.
- the first electrode is integrated in a wall of the container or formed by a wall of the container.
- the second electrode is formed from the bottom of the container as a further structural simplification. A tissue sample taken from the patient can then be placed anywhere in the nutrient solution within the container. A direct positioning on the second electrode is therefore not necessary.
- the sole FIGURE shows a diagnostic device 1, which comprises a container 2, which is filled with an acidic, preferably buffered nutrient solution 3 (measuring solution).
- a nutrient solution 3 (measuring solution).
- the nutrient solution 3 urea is added.
- the diagnostic device 1 further comprises a first electrode 4 (reference electrode) made of a noble metal which is not vulnerable to hydrochloric acid, and a second electrode 5 (measuring electrode) made of silver (Ag).
- the second electrode 5 has on its surface a silver chloride layer (AgCl layer) and is thus activated for the measurement for the detection of Helicobacter pylori.
- platinum (Pt) and gold (Au) come into question.
- the first electrode 4 and the second electrode 5 are at least partially immersed in the container 2.
- a tissue sample 6 (biopsy) is introduced, which was removed by means of a biopsy of the gastric mucosa.
- a voltage U can be applied for a predeterminable time, whereby, when ammonia is present, a change in an electrical quantity, e.g., between the first electrode 4 and the second electrode 5 occurs. Potential, electrical current or electrical resistance, is measurable.
- the first electrode 4 (reference electrode) is integrated in a wall 7 of the container 2 and the second electrode 5 (measuring electrode) is formed from the bottom 8 of the container 2.
- the illustrated embodiment of the diagnostic device according to the invention is designed to be structurally particularly simple. The tissue sample 6 removed from the patient can then be introduced into the acidic nutrient solution 3 within the container 2 in an advantageous manner. A direct positioning of the tissue sample 6 on the second electrode 5 is therefore not necessary.
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
Description
Claims
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE200910009292 DE102009009292A1 (de) | 2009-02-17 | 2009-02-17 | Diagnosegerät |
DE200910023035 DE102009023035A1 (de) | 2009-05-28 | 2009-05-28 | Diagnosegerät |
DE102010006972A DE102010006972A1 (de) | 2010-02-05 | 2010-02-05 | Diagnosegerät |
PCT/EP2010/051851 WO2010094649A1 (de) | 2009-02-17 | 2010-02-15 | Diagnosegerät |
Publications (1)
Publication Number | Publication Date |
---|---|
EP2399128A1 true EP2399128A1 (de) | 2011-12-28 |
Family
ID=42125020
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP10704554A Withdrawn EP2399128A1 (de) | 2009-02-17 | 2010-02-15 | Diagnosegerät |
Country Status (7)
Country | Link |
---|---|
US (1) | US20120077258A1 (de) |
EP (1) | EP2399128A1 (de) |
JP (1) | JP5372179B2 (de) |
CN (1) | CN102317774B (de) |
BR (1) | BRPI1008706A2 (de) |
MX (1) | MX2011008678A (de) |
WO (1) | WO2010094649A1 (de) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015050234A1 (ja) * | 2013-10-02 | 2015-04-09 | 味の素株式会社 | アンモニア制御装置およびアンモニア制御方法 |
DE102013227086A1 (de) | 2013-12-23 | 2015-06-25 | Siemens Aktiengesellschaft | Biopsiezange |
WO2015180747A1 (de) | 2014-05-26 | 2015-12-03 | Siemens Aktiengesellschaft | Messvorrichtung zur ammoniakdetektion in magensaft |
WO2015180748A1 (de) | 2014-05-26 | 2015-12-03 | Siemens Aktiengesellschaft | Messvorrichtung zur ammoniakdetektion in magensaft |
WO2016000756A1 (de) | 2014-07-01 | 2016-01-07 | Siemens Aktiengesellschaft | Messvorrichtung zur ammoniakdetektion in magensaft |
WO2016037662A1 (en) | 2014-09-12 | 2016-03-17 | Siemens Aktiengesellschaft | A helicobacter pylori sensor with ph sensor |
CN111857858A (zh) * | 2019-04-30 | 2020-10-30 | 上海掌门科技有限公司 | 用于处理信息的方法和设备 |
US20220214302A1 (en) * | 2019-05-20 | 2022-07-07 | National Institute For Materials Science | Detection apparatus and data collection method |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0764398B2 (ja) * | 1990-04-13 | 1995-07-12 | 株式会社タツノ・メカトロニクス | ポンプ装置のバイパス弁 |
JPH07184686A (ja) * | 1993-12-28 | 1995-07-25 | Nec Corp | 細胞活性測定方法 |
US5593851A (en) * | 1994-04-01 | 1997-01-14 | Chek-Med Systems, Inc. | Test kid for the rapid detection of helicobacter pylori in gastric biopsy tissue |
JPH07289289A (ja) * | 1994-04-27 | 1995-11-07 | Gastec:Kk | ヘリコバクター・ピロリの簡易検査法及び検査器具 |
JPH08242885A (ja) * | 1995-03-07 | 1996-09-24 | Nec Corp | 細胞活性測定方法 |
JPH09206095A (ja) * | 1996-02-06 | 1997-08-12 | Chem Kiki Kk | ヘリコバクター・ピロリの存否確認方法及び装置 |
US6107080A (en) * | 1996-04-25 | 2000-08-22 | Mcgill University | Biosensor device and method |
JPH11318490A (ja) * | 1998-05-19 | 1999-11-24 | Nippon Koden Corp | ヘリコバクターピロリの検出方法 |
JP2001004581A (ja) * | 1999-06-24 | 2001-01-12 | Sentan Kagaku Gijutsu Incubation Center:Kk | 微小参照電極 |
JP3594230B2 (ja) * | 1999-10-14 | 2004-11-24 | 日本電信電話株式会社 | 電気化学検出器及び検出方法 |
WO2002042766A2 (en) * | 2000-10-26 | 2002-05-30 | University Of Connecticut | A system and method for investigating the effect of chemical and other factors on cell movement |
DE10142253C1 (de) | 2001-08-29 | 2003-04-24 | Siemens Ag | Endoroboter |
JP4230430B2 (ja) * | 2003-09-25 | 2009-02-25 | 富士通株式会社 | 被検体評価装置および被検体評価方法 |
JP2007003408A (ja) * | 2005-06-24 | 2007-01-11 | Kyushu Institute Of Technology | 細胞バイオセンサ |
EP1785085A1 (de) * | 2005-11-12 | 2007-05-16 | Roche Diagnostics GmbH | Implantierbares Elektrodensystem, Verfahren und Vorrichtung zum Messen einer Analytkonzentration in einem menschlichen oder tierischen Körper |
WO2008054611A2 (en) | 2006-10-04 | 2008-05-08 | President And Fellows Of Harvard College | Engineered conductive polymer films to mediate biochemical interactions |
-
2010
- 2010-02-15 BR BRPI1008706A patent/BRPI1008706A2/pt not_active IP Right Cessation
- 2010-02-15 MX MX2011008678A patent/MX2011008678A/es active IP Right Grant
- 2010-02-15 WO PCT/EP2010/051851 patent/WO2010094649A1/de active Application Filing
- 2010-02-15 JP JP2011549586A patent/JP5372179B2/ja not_active Expired - Fee Related
- 2010-02-15 US US13/146,701 patent/US20120077258A1/en not_active Abandoned
- 2010-02-15 EP EP10704554A patent/EP2399128A1/de not_active Withdrawn
- 2010-02-15 CN CN201080008118.9A patent/CN102317774B/zh not_active Expired - Fee Related
Non-Patent Citations (1)
Title |
---|
See references of WO2010094649A1 * |
Also Published As
Publication number | Publication date |
---|---|
US20120077258A1 (en) | 2012-03-29 |
BRPI1008706A2 (pt) | 2016-03-08 |
JP2012518161A (ja) | 2012-08-09 |
MX2011008678A (es) | 2011-09-08 |
CN102317774A (zh) | 2012-01-11 |
JP5372179B2 (ja) | 2013-12-18 |
WO2010094649A1 (de) | 2010-08-26 |
CN102317774B (zh) | 2014-10-08 |
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