EA201491821A1 - Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот - Google Patents
Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислотInfo
- Publication number
- EA201491821A1 EA201491821A1 EA201491821A EA201491821A EA201491821A1 EA 201491821 A1 EA201491821 A1 EA 201491821A1 EA 201491821 A EA201491821 A EA 201491821A EA 201491821 A EA201491821 A EA 201491821A EA 201491821 A1 EA201491821 A1 EA 201491821A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- functional groups
- modified oligonucleotides
- detection
- carbon
- thiol functional
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
- C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Communicable Diseases (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Изобретение относится к модифицированным олигонуклеотидам, имеющим две или более тиольных функциональных группы, которые могут быть зафиксированы на золотой поверхности или на привитой поверхности, в частности на поверхности, включающей по меньшей мере одну углерод-углеродную двойную связь, или углерод-углеродную тройную связь, или галогенацетамидные группы, предпочтительно малеимидные или акриламидные функциональные группы. Изобретение также относится к способу детекции нуклеиновой кислоты в биологическом образце, включающему стадию детекции гибридизации между модифицированным олигонуклеотидом и нуклеиновой кислотой-мишенью, амплифицированной из биологического образца. Изобретение относится, в частности, к способу детекции, генотипирования или секвенирования патогенного организма, предпочтительно вируса.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1253122A FR2989089B1 (fr) | 2012-04-04 | 2012-04-04 | Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques |
PCT/EP2013/057150 WO2013150122A1 (fr) | 2012-04-04 | 2013-04-04 | Oligonucleotides modifies compenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques |
Publications (2)
Publication Number | Publication Date |
---|---|
EA201491821A1 true EA201491821A1 (ru) | 2015-02-27 |
EA029907B1 EA029907B1 (ru) | 2018-05-31 |
Family
ID=48128279
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201491821A EA029907B1 (ru) | 2012-04-04 | 2013-04-04 | Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот |
Country Status (12)
Country | Link |
---|---|
US (2) | US20150232957A1 (ru) |
EP (1) | EP2859008B1 (ru) |
JP (1) | JP6175489B2 (ru) |
KR (1) | KR102186143B1 (ru) |
AU (1) | AU2013244900B2 (ru) |
BR (1) | BR112014024601B1 (ru) |
CA (1) | CA2868926C (ru) |
EA (1) | EA029907B1 (ru) |
ES (1) | ES2624993T3 (ru) |
FR (1) | FR2989089B1 (ru) |
IN (1) | IN2014DN08170A (ru) |
WO (1) | WO2013150122A1 (ru) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3472376A4 (en) | 2016-06-16 | 2019-12-18 | Richard Edward Watts | DIRECTED AND RECORDED COMBINATORY SYNTHESIS OF OLIGONUCLEOTIDES OF CODED PROBE MOLECULES |
EP3619340A4 (en) | 2017-05-02 | 2021-01-20 | Haystack Sciences Corporation | MOLECULES FOR THE VERIFICATION OF OLIGONUCLEOTIDE DIRECTED COMBINATIONAL SYNTHESIS AND METHOD OF MANUFACTURING AND USING THEREOF |
WO2019039403A1 (ja) * | 2017-08-22 | 2019-02-28 | 国立大学法人名古屋大学 | 修飾ポリヌクレオチド |
CN111295444A (zh) * | 2017-09-25 | 2020-06-16 | 哈斯达克科学公司 | 用于寡核苷酸指导的组合化学的多项式编码 |
CA3211098A1 (en) | 2021-03-09 | 2022-09-15 | Chantal Fournier-Wirth | Detection of infectious agent based on recombinase polymerase amplification combined with a magnetic field-enhanced agglutination |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6041183A (ja) * | 1983-08-16 | 1985-03-04 | 三菱電機株式会社 | 入場管理装置 |
JP2899111B2 (ja) * | 1991-07-15 | 1999-06-02 | ラ ホヤ ファーマシューティカル カンパニー | オリゴヌクレオチドの5’末端へ官能基を提供するための修飾された亜リン酸中間体 |
JPH0641183A (ja) * | 1992-07-23 | 1994-02-15 | Mitsubishi Kasei Corp | オリゴヌクレオチド単分子膜 |
DE10163836A1 (de) * | 2001-12-22 | 2003-07-10 | Friz Biochem Gmbh | Multifunktionales Reagenz zur Synthese von thiolmodifizierten Oligomeren |
US7371585B2 (en) * | 2003-12-31 | 2008-05-13 | Genencor International, Inc. | Membranes incorporating recognition moieties |
US7167395B1 (en) | 2005-07-13 | 2007-01-23 | Infineon Technologies Flash Gmbh & Co. Kg | Non-volatile semiconductor memory |
MX2008008237A (es) | 2005-12-23 | 2008-11-06 | Siemens Healthcare Diagnostics | Metodos y reactivos para genotipificar hcv. |
FR2989086B1 (fr) | 2012-04-04 | 2017-03-24 | Centre Nat De La Rech Scient (Cnrs) | Composes thiol et leur utilisation pour la synthese d'oligonucleotides modifies |
-
2012
- 2012-04-04 FR FR1253122A patent/FR2989089B1/fr active Active
-
2013
- 2013-04-04 BR BR112014024601-7A patent/BR112014024601B1/pt active IP Right Grant
- 2013-04-04 AU AU2013244900A patent/AU2013244900B2/en active Active
- 2013-04-04 ES ES13716756.5T patent/ES2624993T3/es active Active
- 2013-04-04 JP JP2015503888A patent/JP6175489B2/ja active Active
- 2013-04-04 CA CA2868926A patent/CA2868926C/fr active Active
- 2013-04-04 US US14/390,748 patent/US20150232957A1/en not_active Abandoned
- 2013-04-04 WO PCT/EP2013/057150 patent/WO2013150122A1/fr active Application Filing
- 2013-04-04 EP EP13716756.5A patent/EP2859008B1/fr active Active
- 2013-04-04 KR KR1020147027987A patent/KR102186143B1/ko active IP Right Grant
- 2013-04-04 IN IN8170DEN2014 patent/IN2014DN08170A/en unknown
- 2013-04-04 EA EA201491821A patent/EA029907B1/ru not_active IP Right Cessation
-
2019
- 2019-02-08 US US16/271,581 patent/US11390643B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
KR102186143B1 (ko) | 2020-12-03 |
US20190376151A1 (en) | 2019-12-12 |
AU2013244900A1 (en) | 2014-10-16 |
US11390643B2 (en) | 2022-07-19 |
BR112014024601A2 (pt) | 2018-04-17 |
FR2989089B1 (fr) | 2020-02-07 |
EP2859008A1 (fr) | 2015-04-15 |
AU2013244900B2 (en) | 2017-01-12 |
BR112014024601B1 (pt) | 2022-05-24 |
ES2624993T3 (es) | 2017-07-18 |
WO2013150122A1 (fr) | 2013-10-10 |
US20150232957A1 (en) | 2015-08-20 |
EA029907B1 (ru) | 2018-05-31 |
FR2989089A1 (fr) | 2013-10-11 |
IN2014DN08170A (ru) | 2015-05-01 |
JP6175489B2 (ja) | 2017-08-02 |
CA2868926A1 (fr) | 2013-10-10 |
CA2868926C (fr) | 2021-05-04 |
EP2859008B1 (fr) | 2017-02-22 |
JP2015514402A (ja) | 2015-05-21 |
KR20150021493A (ko) | 2015-03-02 |
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PD4A | Registration of transfer of a eurasian patent in accordance with the succession in title | ||
MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG TJ TM |