DK1766040T3 - Fremgangsmåder til transformering og ekspressions-screening af filamentøse svampeceller med et DNA-bibliotek - Google Patents
Fremgangsmåder til transformering og ekspressions-screening af filamentøse svampeceller med et DNA-bibliotekInfo
- Publication number
- DK1766040T3 DK1766040T3 DK05786779.8T DK05786779T DK1766040T3 DK 1766040 T3 DK1766040 T3 DK 1766040T3 DK 05786779 T DK05786779 T DK 05786779T DK 1766040 T3 DK1766040 T3 DK 1766040T3
- Authority
- DK
- Denmark
- Prior art keywords
- transforming
- expressing
- screening
- methods
- dna library
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Virology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US57560004P | 2004-05-27 | 2004-05-27 | |
| PCT/US2005/018871 WO2005121351A2 (en) | 2004-05-27 | 2005-05-26 | Methods for transforming and expression screening of filamentous fungal cells with a dna library |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1766040T3 true DK1766040T3 (da) | 2017-11-13 |
Family
ID=35503731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK05786779.8T DK1766040T3 (da) | 2004-05-27 | 2005-05-26 | Fremgangsmåder til transformering og ekspressions-screening af filamentøse svampeceller med et DNA-bibliotek |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9045748B2 (da) |
| EP (1) | EP1766040B1 (da) |
| CN (1) | CN1993475A (da) |
| CA (1) | CA2565888C (da) |
| DK (1) | DK1766040T3 (da) |
| WO (1) | WO2005121351A2 (da) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE503018T1 (de) * | 2006-06-26 | 2011-04-15 | Dsm Ip Assets Bv | Transektion filamentöser pilze mit hohem durchsatz |
| KR101098032B1 (ko) * | 2008-04-22 | 2011-12-23 | 한국생명공학연구원 | 4차 연속 피씨알, 4차 블록 피씨알, 또는 유전자 합성방법을 이용한 균주 특이적 바코드를 포함하는 유전자 적중 이형접합체 분열효모 균주의 제조방법 |
| JP5972793B2 (ja) | 2009-12-23 | 2016-08-17 | アフィニティ バイオサイエンス ピーティーワイ リミテッド | タンパク質ディスプレイ |
| CA2840650C (en) | 2011-06-29 | 2019-08-20 | Affinity Biosciences Pty Ltd | Method of protein display |
| WO2014093275A1 (en) | 2012-12-12 | 2014-06-19 | Danisco Us Inc. | Variants of cellobiohydrolases |
| CN104789555A (zh) * | 2015-04-29 | 2015-07-22 | 江南大学 | 一种基于合成单链dna文库进化基因表达调控元件的方法 |
| WO2019046703A1 (en) | 2017-09-01 | 2019-03-07 | Novozymes A/S | METHODS OF ENHANCING GENOME EDITION IN FUNGI |
| EP3853239A4 (en) * | 2018-09-20 | 2021-12-22 | Perfect Day, Inc. | METHODS AND COMPOSITIONS FOR MANUFACTURING HOMOCARYOTIC FILAMENTOUS FUNGAL CELLS |
| EP3640331A1 (en) * | 2018-10-18 | 2020-04-22 | Basf Se | Method for analyzing a library |
| WO2024023343A1 (en) | 2022-07-28 | 2024-02-01 | Mushlabs Gmbh | Method and system for fungal culture and characteristics prediction of mycelium derived products |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4816405A (en) | 1984-10-24 | 1989-03-28 | The Regents Of The University Of California | Vectors for transformation by ascomycetes |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5364770A (en) * | 1985-08-29 | 1994-11-15 | Genencor International Inc. | Heterologous polypeptides expressed in aspergillus |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| DE69033732T2 (de) | 1990-01-08 | 2001-10-11 | Stratagene, La Jolla | Neue Wirtsorganismen zur Klonierung |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| US5512468A (en) | 1993-11-12 | 1996-04-30 | Stratagene | Process of producing highly transformable bacterial cells and cells produced thereby |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| DK0765394T3 (da) | 1994-06-03 | 2001-12-10 | Novozymes As | Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse |
| AU2705895A (en) | 1994-06-30 | 1996-01-25 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| AU6655496A (en) | 1995-08-11 | 1997-03-12 | Novo Nordisk A/S | Method for preparing polypeptide variants |
| CN1226283A (zh) | 1996-06-28 | 1999-08-18 | 诺沃挪第克公司 | 具有葡聚糖酶活性的重组酶 |
| DE69840382D1 (de) | 1997-03-18 | 2009-02-05 | Novozymes As | Methode zur herstellung einer bibliothek mittels dna-shuffling |
| AU738461B2 (en) | 1997-03-18 | 2001-09-20 | Novozymes A/S | Shuffling of heterologous DNA sequences |
| DE69838106T2 (de) * | 1997-12-22 | 2008-04-03 | Dsm Ip Assets B.V. | Expressionsklonierung in filamentösen pilzen |
| CN1307642A (zh) | 1998-05-27 | 2001-08-08 | 诺沃诺尔迪斯克生物技术有限公司 | 通过改变基因拷贝数制备多肽的方法 |
| US6040184A (en) | 1998-10-09 | 2000-03-21 | Stratagene | Method for more efficient electroporation |
| CA2344619C (en) * | 1998-10-26 | 2012-01-03 | Novozymes A/S | Constructing and screening a dna library of interest in filamentous fungal cells |
| US6723504B1 (en) | 1998-10-28 | 2004-04-20 | Novozymes A/S | Method for generating a gene library |
| US20030054390A1 (en) | 1999-01-19 | 2003-03-20 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6299850B1 (en) * | 1999-03-16 | 2001-10-09 | The United States Of America As Represented By The Department Of Energy | Carbon activation process for increased surface accessibility in electrochemical capacitors |
| CN100510096C (zh) | 1999-03-22 | 2009-07-08 | 诺沃奇梅兹有限公司 | 用于在真菌细胞中表达基因的启动子 |
| AU1783901A (en) | 1999-11-30 | 2001-06-12 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide using a consensus translational initiator sequence |
| KR20020026456A (ko) | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝 |
| WO2002059331A1 (en) | 2001-01-24 | 2002-08-01 | Novozymes A/S | In vivo mutation and recombination in filamentous fungi |
-
2005
- 2005-05-26 EP EP05786779.8A patent/EP1766040B1/en not_active Not-in-force
- 2005-05-26 CA CA2565888A patent/CA2565888C/en not_active Expired - Fee Related
- 2005-05-26 CN CNA2005800255512A patent/CN1993475A/zh active Pending
- 2005-05-26 DK DK05786779.8T patent/DK1766040T3/da active
- 2005-05-26 US US11/140,150 patent/US9045748B2/en not_active Expired - Fee Related
- 2005-05-26 WO PCT/US2005/018871 patent/WO2005121351A2/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| US20060172379A1 (en) | 2006-08-03 |
| EP1766040B1 (en) | 2017-08-09 |
| US9045748B2 (en) | 2015-06-02 |
| WO2005121351A2 (en) | 2005-12-22 |
| CA2565888A1 (en) | 2005-12-22 |
| EP1766040A2 (en) | 2007-03-28 |
| WO2005121351A3 (en) | 2006-04-13 |
| EP1766040A4 (en) | 2008-12-31 |
| CN1993475A (zh) | 2007-07-04 |
| CA2565888C (en) | 2014-10-14 |
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