DE19702754A1 - Laser-scan-microscope filter changer - Google Patents
Laser-scan-microscope filter changerInfo
- Publication number
- DE19702754A1 DE19702754A1 DE1997102754 DE19702754A DE19702754A1 DE 19702754 A1 DE19702754 A1 DE 19702754A1 DE 1997102754 DE1997102754 DE 1997102754 DE 19702754 A DE19702754 A DE 19702754A DE 19702754 A1 DE19702754 A1 DE 19702754A1
- Authority
- DE
- Germany
- Prior art keywords
- filter
- wheel
- filters
- changer according
- microscope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
- G02B26/007—Optical devices or arrangements for the control of light using movable or deformable optical elements the movable or deformable optical element controlling the colour, i.e. a spectral characteristic, of the light
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/006—Filter holders
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Astronomy & Astrophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
Anwendungsgebiet der Erfindung sind Mikroskope, insbesondere Laser-Scan-Mikroskope, beispielsweise gemäß DE 43 23 129 A1. Derartige Systeme enthalten einen oder mehrere Detektionskanäle, in denen auswechselbare Filter zur wellenlängenabhängigen, breit- oder schmalbandigen Detektion angeordnet sind.Field of application of the invention are microscopes, in particular Laser scan microscopes, for example according to DE 43 23 129 A1. Such systems contain one or more Detection channels in which replaceable filters for Wavelength-dependent, broadband or narrowband detection are arranged.
Zur Auswechslung dieser Filter ist es bekannt, motorisch oder von Hand betriebene Filterräder zu verwenden, die verschiedene Filter nacheinander in den Strahlengang einschwenken (Handbook of biological confocal microscopy, Second Edition 1995, Plenum Press, London, New York).To replace these filters it is known to be motor or to use hand-operated filter wheels, the different Swing the filter into the beam path one after the other (Handbook of biological confocal microscopy, Second Edition 1995, Plenum Press, London, New York).
Die Zahl der Filter ist durch das Filterrad vorgegeben. Will man andere Wellenlängen untersuchen, muß man ein neues Filterrad einsetzen.The number of filters is determined by the filter wheel. If you want to examine other wavelengths, you have to find a new one Insert the filter wheel.
Hierdurch ist eine völlige Neujustierung erforderlich.This requires a complete readjustment.
Aufgabe der Erfindung ist es, diesen Nachteil zu vermeiden. Dies erfolgt gemäß der Patentansprüche, indem mindestens eine Position auf dem Filterrad separat auswechselbar gestaltet ist. Hierdurch kann ein einzelner Filter reproduzierbar ausgewechselt werden, d. h. ohne zusätzlichen Justiervorgang. Vorteilhaft ist, daß hierdurch eine Vielzahl von Filtern ohne großen technischen Aufwand in den Detektionsstrahlengang eingesetzt werden kann.The object of the invention is to avoid this disadvantage. This is done according to the claims by at least one Position on the filter wheel is designed separately replaceable. This allows a single filter to be reproduced be replaced, d. H. without additional adjustment. It is advantageous that a large number of filters without great technical effort in the detection beam path can be used.
Bei Ausbildung dieser Position als gegenüber standardmäßigen Filtern standardisierte Auswechselstelle ist dies besonders vorteilhaft möglich.When training this position as compared to standard Filter standardized exchange point this is special advantageously possible.
Durch eine zum Mikroskop gehörende Ansteuereiheit wird das Filterrad motorisch verstellt, bis die Eingabestelle für einen neuen Filter positionsmäßig mit der Auswechselstelle des Filterrades übereinstimmt.This is achieved by a control unit belonging to the microscope Filter wheel adjusted by motor until the entry point for position a new filter with the replacement point of the Filter wheel matches.
Die Auswechslung der Filter erfolgt mit einer Filterfassung gemeinsam, wobei der Filter in der Filterfassung reproduzierbar und vorteilhaft drehbar gehaltert ist und die Filterfassung durch geeignete mechanische und/oder beispielsweise magnetische Hilfsmittel reproduzierbar am Filterrad gehaltert ist.The filters are replaced with a filter holder together, the filter reproducible in the filter holder and is advantageously rotatably supported and the filter holder by suitable mechanical and / or for example magnetic aids reproducibly held on the filter wheel is.
Durch die drehbare Lagerung der Filter, deren Beschichtung meist nicht völlig über ihre Fläche konstant ist, kann bei gleichzeitiger exzentrischer Lichtwegsführung bezüglich des Filtermittelpunktes durch eine Drehung des Filters vorteilhaft die Stelle mit der besten Intensität für die durchgelassenen Wellenlänge oder den Wellenlängenbereich durch Drehung ermittelt werden, was vor allem bei der Untersuchung schwach emittierender Proben eine entscheidende Rolle spielen kann.Due to the rotatable mounting of the filter and its coating is usually not completely constant over its area, can simultaneous eccentric light path with respect to the Filter center point advantageous by rotating the filter the place with the best intensity for the let through Wavelength or the wavelength range by rotation be determined what is weak especially when examining emitting samples can play a crucial role.
Weitere Vorteile und die Funktionsweise der Erfindung werden nachstehend anhand der Zeichnungen näher erläutert.Further advantages and the functioning of the invention will be explained below with reference to the drawings.
Es zeigen:Show it:
Fig. 1 Eine räumliche Darstellung eines in einem Mikroskopgehäuse gehalterten Filterrades, Fig. 1 is a perspective view of a housing retained in a microscope filter wheel,
Fig. 2 Einen Querschnitt durch das Filterrad, Fig. 2 shows a cross section through the filter,
Fig. 3 Eine räumliche Darstellung mit eingeschobener Filterfassung. Fig. 3 A spatial representation with the filter holder inserted.
An einem gerätefesten Träger list ein mittels eines Schrittmotors 2 angetriebenes Filterrad 3 mit einer Drehachse 4 befestigt, das mehrere spektrale Filter 5 aufweist.A filter wheel 3 , which is driven by means of a stepping motor 2 and has an axis of rotation 4 and which has a plurality of spectral filters 5 , is fastened to a device-fixed carrier.
Ein Führungsschacht 6 mit abnehmbaren Verschlußdeckel 7, der die Lichtdichte gewährleistet, dient dem Einschub bzw. der Entnahme von auswechselbaren Filterfassungen 8.A guide shaft 6 with a removable cover 7 , which ensures the light density, is used to insert or remove interchangeable filter holders 8 .
Der Schrittmotor 2 ist mit einem Positionsgeber 9 verbunden, so daß eine eindeutige Erfassung der Stellung des Filterrades 3 ermöglicht wird. The stepper motor 2 is connected to a position sensor 9 , so that a clear detection of the position of the filter wheel 3 is made possible.
Im Filterrad 3 ist eine Aufnahme 10 vorgesehen, die in der Auswechselstellung mit dem Führungsschacht 6 fluchtet, so daß von außen, beispielsweise mittels einer Bedienzange, die in Führungen 11 eingreift, eine Auswechslung der Filterfassung 8 mit eingesetztem Filter erfolgen kann.In the filter wheel 3 , a receptacle 10 is provided which is aligned with the guide shaft 6 in the replacement position, so that the filter holder 8 with the filter inserted can be replaced from the outside, for example by means of operating pliers which engage in guides 11 .
Hierbei wird die Orientierung des Filterrades 3 im Detektionsstrahlengang 12, der hier schematisch durch eine exzentrisch zur Filtermitte verlaufende Strichlinie 12 dargestellt ist, vorteilhaft beibehalten.In this case, the orientation of the filter wheel 3 in the detection beam path 12 , which is represented here schematically by a dashed line 12 running eccentrically to the filter center, is advantageously maintained.
Durch drehbare Anordnung der Filter in der Filterfassung 8 kann bei der Justierung die Filterstelle mit der für die verwendete Wellenlänge höchsten Durchlässigkeit eingestellt werden. Die erfindungsgemäß ausgebildeten Filterräder 3 können auch in mehreren Detektionskanälen vorgesehen sein.Due to the rotatable arrangement of the filters in the filter holder 8 , the filter point with the highest transmission for the wavelength used can be adjusted during the adjustment. The filter wheels 3 designed according to the invention can also be provided in a plurality of detection channels.
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE1997102754 DE19702754B4 (en) | 1997-01-27 | 1997-01-27 | Filter changer for microscopes |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE1997102754 DE19702754B4 (en) | 1997-01-27 | 1997-01-27 | Filter changer for microscopes |
Publications (2)
Publication Number | Publication Date |
---|---|
DE19702754A1 true DE19702754A1 (en) | 1998-07-30 |
DE19702754B4 DE19702754B4 (en) | 2007-10-04 |
Family
ID=7818406
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE1997102754 Expired - Fee Related DE19702754B4 (en) | 1997-01-27 | 1997-01-27 | Filter changer for microscopes |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE19702754B4 (en) |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004031825A1 (en) * | 2002-09-25 | 2004-04-15 | Olympus Biosystems Gmbh | Filter holder |
US6791767B2 (en) | 2002-09-16 | 2004-09-14 | Leica Microsystems, Inc. | Magnetic optical element holder and microscope assembly including same |
EP1460465A1 (en) * | 2003-03-19 | 2004-09-22 | Prodisc Technology Inc. | Colour filter wheel assembly and colour filter wheel thereof |
GB2416261A (en) * | 2005-05-21 | 2006-01-18 | Zeiss Carl Jena Gmbh | Laser scanning microscope with parallel illumination and simultaneous, locally resolved detection |
GB2416440A (en) * | 2004-07-16 | 2006-01-25 | Zeiss Carl Jena Gmbh | Laser scanning microscope with parallel illumination via distributed point like light sources |
US7170694B2 (en) | 2002-11-01 | 2007-01-30 | Leica Microsystems Ag (Schweiz) | Magnetically positioned precision holder for optical components |
WO2007028519A1 (en) * | 2005-09-10 | 2007-03-15 | Carl Zeiss Jena Gmbh | Device for the replacement of optical elements in beam paths |
WO2007134698A1 (en) * | 2006-05-19 | 2007-11-29 | Carl Zeiss Microimaging Gmbh | Microscope with a changing device for optical elements |
DE102009011681A1 (en) | 2009-02-23 | 2010-08-26 | Obrebski, Andreas, Dr. | Changer for optical elements |
CN102313956A (en) * | 2011-08-26 | 2012-01-11 | 深圳市优威视讯科技有限公司 | Optical device automatic switching device and photography equipment |
WO2013026959A1 (en) * | 2011-08-24 | 2013-02-28 | Labrox Oy | Microtiter plate reader apparatus and dynamic filter storage |
DE102012019472A1 (en) | 2012-09-28 | 2014-04-03 | Carl Zeiss Microscopy Gmbh | Optical filter device, in particular for microscopes |
US8786944B2 (en) | 2011-03-25 | 2014-07-22 | Leica Microsystems Cms Gmbh | Device for holding filters for microscopes |
US20150260938A1 (en) * | 2013-01-04 | 2015-09-17 | Cellomics, Inc. | Optical quickmount |
CN111897080A (en) * | 2020-08-03 | 2020-11-06 | 苏州慧导昱控自动化科技有限公司 | Optical special mould |
CN112130269A (en) * | 2020-09-27 | 2020-12-25 | 浙江华疆科技有限公司 | Automatic filter replacing device of exposure machine |
DE102022200950A1 (en) | 2022-01-28 | 2023-08-03 | Carl Zeiss Microscopy Gmbh | Optics changer for feeding a number of optical elements into a beam path and microscope |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102014116648B4 (en) * | 2014-08-20 | 2017-03-30 | Leica Microsystems Cms Gmbh | Microscope with two access openings for fluorescence device |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4323129C2 (en) * | 1992-07-24 | 2002-10-02 | Zeiss Carl | Inverted microscope |
DE4404286C2 (en) * | 1994-02-11 | 1996-01-25 | Leica Mikroskopie & Syst | Fluorescence device for an inverse microscope |
-
1997
- 1997-01-27 DE DE1997102754 patent/DE19702754B4/en not_active Expired - Fee Related
Cited By (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6791767B2 (en) | 2002-09-16 | 2004-09-14 | Leica Microsystems, Inc. | Magnetic optical element holder and microscope assembly including same |
EP1540402A2 (en) * | 2002-09-16 | 2005-06-15 | Leica Microsystems, Inc. | Magnetic optical element holder and microscope assembly including same |
EP1540402A4 (en) * | 2002-09-16 | 2006-10-25 | Leica Microsystems | Magnetic optical element holder and microscope assembly including same |
WO2004031825A1 (en) * | 2002-09-25 | 2004-04-15 | Olympus Biosystems Gmbh | Filter holder |
US7170694B2 (en) | 2002-11-01 | 2007-01-30 | Leica Microsystems Ag (Schweiz) | Magnetically positioned precision holder for optical components |
EP1460465A1 (en) * | 2003-03-19 | 2004-09-22 | Prodisc Technology Inc. | Colour filter wheel assembly and colour filter wheel thereof |
GB2416440A (en) * | 2004-07-16 | 2006-01-25 | Zeiss Carl Jena Gmbh | Laser scanning microscope with parallel illumination via distributed point like light sources |
GB2416261A (en) * | 2005-05-21 | 2006-01-18 | Zeiss Carl Jena Gmbh | Laser scanning microscope with parallel illumination and simultaneous, locally resolved detection |
WO2007028519A1 (en) * | 2005-09-10 | 2007-03-15 | Carl Zeiss Jena Gmbh | Device for the replacement of optical elements in beam paths |
WO2007134698A1 (en) * | 2006-05-19 | 2007-11-29 | Carl Zeiss Microimaging Gmbh | Microscope with a changing device for optical elements |
DE102009011681A1 (en) | 2009-02-23 | 2010-08-26 | Obrebski, Andreas, Dr. | Changer for optical elements |
WO2010094279A2 (en) | 2009-02-23 | 2010-08-26 | Andreas Obrebski | Converter for optical elements |
US8675285B2 (en) | 2009-02-23 | 2014-03-18 | Andreas Obrebski | Changer for optical elements |
US8786944B2 (en) | 2011-03-25 | 2014-07-22 | Leica Microsystems Cms Gmbh | Device for holding filters for microscopes |
WO2013026959A1 (en) * | 2011-08-24 | 2013-02-28 | Labrox Oy | Microtiter plate reader apparatus and dynamic filter storage |
US9354173B2 (en) | 2011-08-24 | 2016-05-31 | Labrox Oy | Microtiter plate reader apparatus and dynamic filter storage |
CN102313956A (en) * | 2011-08-26 | 2012-01-11 | 深圳市优威视讯科技有限公司 | Optical device automatic switching device and photography equipment |
US8988753B2 (en) | 2012-09-28 | 2015-03-24 | Carl Zeiss Microscopy Gmbh | Optical filter device, in particular for microscopes |
DE102012019472A1 (en) | 2012-09-28 | 2014-04-03 | Carl Zeiss Microscopy Gmbh | Optical filter device, in particular for microscopes |
DE102012019472B4 (en) | 2012-09-28 | 2023-05-04 | Carl Zeiss Microscopy Gmbh | Optical filter device, in particular for microscopes, and microscope |
US20150260938A1 (en) * | 2013-01-04 | 2015-09-17 | Cellomics, Inc. | Optical quickmount |
US9400365B2 (en) * | 2013-01-04 | 2016-07-26 | Cellomics, Inc. | Optical quickmount |
CN111897080A (en) * | 2020-08-03 | 2020-11-06 | 苏州慧导昱控自动化科技有限公司 | Optical special mould |
CN112130269A (en) * | 2020-09-27 | 2020-12-25 | 浙江华疆科技有限公司 | Automatic filter replacing device of exposure machine |
CN112130269B (en) * | 2020-09-27 | 2022-12-30 | 浙江华疆科技有限公司 | Automatic filter replacing device of exposure machine |
DE102022200950A1 (en) | 2022-01-28 | 2023-08-03 | Carl Zeiss Microscopy Gmbh | Optics changer for feeding a number of optical elements into a beam path and microscope |
Also Published As
Publication number | Publication date |
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DE19702754B4 (en) | 2007-10-04 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8110 | Request for examination paragraph 44 | ||
8364 | No opposition during term of opposition | ||
R081 | Change of applicant/patentee |
Effective date: 20130206 Free format text: FORMER OWNER: CARL ZEISS JENA GMBH, 07745 JENA, DE Owner name: CARL ZEISS MICROSCOPY GMBH, DE |
|
R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee | ||
R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee |
Effective date: 20140801 |