CN220317818U - Anaerobic bacteria cultivates bucket - Google Patents
Anaerobic bacteria cultivates bucket Download PDFInfo
- Publication number
- CN220317818U CN220317818U CN202321803140.3U CN202321803140U CN220317818U CN 220317818 U CN220317818 U CN 220317818U CN 202321803140 U CN202321803140 U CN 202321803140U CN 220317818 U CN220317818 U CN 220317818U
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- China
- Prior art keywords
- annular
- layer glass
- sealing cover
- anaerobic bacteria
- staving
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- 241001148471 unidentified anaerobic bacterium Species 0.000 title claims abstract description 27
- 238000007789 sealing Methods 0.000 claims abstract description 30
- 238000010438 heat treatment Methods 0.000 claims abstract description 24
- 239000011521 glass Substances 0.000 claims description 38
- 239000012780 transparent material Substances 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000010030 laminating Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000004321 preservation Methods 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 28
- 238000009413 insulation Methods 0.000 description 5
- 239000012774 insulation material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
An anaerobic bacteria culturing barrel belongs to the technical field of microorganism laboratory devices and comprises a barrel body, a sealing cover, a controller, a heating mechanism, a temperature sensor, a gas pressure sensor, a gas concentration sensor, an annular bracket and a limiting groove. The utility model discloses a set up heating mechanism, temperature sensor, gas pressure sensor, gas concentration sensor, controller, temperature, gas pressure, the gas concentration in the rational control staving to provide good culture environment for anaerobe, be favorable to improving cultivation efficiency and cultivation quality, simultaneously, the utility model discloses a rationally set up annular bracket, under the circumstances of make full use of staving inner space, can make the experimenter in time observe the culture state of anaerobe in the culture dish.
Description
Technical Field
The utility model belongs to the technical field of microorganism laboratory device, concretely relates to anaerobic bacteria cultivates bucket.
Background
The anaerobic bacteria cultivation barrel in the laboratory is simple in structure and comprises a sealed glass tank, a clamping seat is arranged in the center of the glass tank and used for clamping mutually overlapped culture dishes, and a culture medium for inoculating anaerobic bacteria is arranged in the culture dishes. An anaerobic gas generating bag is arranged on the periphery of the clamping seat, and oxygen in the glass tank is absorbed by the anaerobic gas generating bag to generate gas suitable for culturing anaerobic bacteria, so that a gas environment suitable for culturing anaerobic bacteria is formed in the glass tank. However, this incubator has the following significant drawbacks:
1. the culture dishes are mutually overlapped in the clamping seat, and experimental staff cannot observe the growth condition of anaerobic bacterial colonies in the shielded culture dishes in real time.
2. The culture barrel has no temperature control function, and the whole device is placed into the culture box to keep constant temperature so as to smoothly complete the culture of anaerobic bacteria.
Disclosure of Invention
In order to overcome the defects in the prior art, the utility model discloses an anaerobic bacteria cultivation barrel, which aims to solve the problems in the background technology parts 1 and 2.
In order to achieve the above purpose, the technical scheme of the utility model is:
the utility model provides an anaerobic bacteria cultivates bucket, includes staving, sealed lid, controller, the staving be equipped with double glazing lateral wall, be equipped with heating mechanism and temperature sensor in the staving, the controller locate the staving outside and be connected with power, temperature sensor, heating mechanism electricity respectively through the wire, the staving in be equipped with a plurality of superimposed annular brackets each other, annular bracket include along the annular bottom plate that the level set up, locate annular bottom plate upper surface outer fringe and along the annular curb plate of vertically upwards extending, the surface and the staving internal surface clearance fit of annular curb plate, evenly distributed has a plurality of spacing grooves on annular bottom plate upper surface, the spacing groove be used for putting transparent culture dish, the top of annular curb plate offset with the lower surface outer fringe of the adjacent annular bottom plate of top, annular curb plate be transparent material and make, the height of annular curb plate satisfies: the culture form of anaerobic bacteria in the culture dish can be seen through the double-layer glass side wall.
Preferably, the side wall of the double-layer glass is of a cylindrical structure and comprises inner-layer glass positioned at an inner layer and outer-layer glass positioned at an outer layer, vacuum pumping is carried out between the inner-layer glass and the outer-layer glass, the top ends of the inner-layer glass and the outer-layer glass are fixedly connected with a fixing ring together, the inner surface of the fixing ring is coplanar with the inner surface of the inner-layer glass, the fixing ring is provided with external threads, and the external threads are in threaded connection with a sealing cover; the bottom of the side wall of the double-layer glass is connected with a base.
Preferably, the heating mechanism comprises a sleeve which is coaxially and fixedly connected with the bottom end of the sealing cover, a heating rod is coaxially arranged in the sleeve, the top end of the heating rod is fixedly connected with the bottom end of the sealing cover through a connecting seat, and a plurality of heat penetrating holes are uniformly distributed on the side wall of the sleeve.
Preferably, the controller is arranged at the center of the top end of the sealing cover and is electrically connected with the heating rod through a wire, and the inner edge of the annular bottom plate is in clearance fit with the outer surface of the sleeve.
Preferably, the sealing cover is also penetrated with an air inlet pipe and an air outlet pipe, and the pipe bodies of the air inlet pipe and the air outlet pipe above the sealing cover are respectively provided with a regulating valve.
Preferably, a temperature sensor and a gas concentration sensor are arranged at the bottom end of the sealing cover, and the gas concentration sensor is electrically connected with the controller through a wire.
Preferably, the bottom end of the sealing cover is also provided with a gas pressure sensor, and the gas pressure sensor is electrically connected with the controller through a wire.
Preferably, the upper surface of the base is also provided with an insulation board, the upper surface of the insulation board is attached to the lower surface of the annular bottom plate at the bottommost layer, and the sealing cover is provided with an insulation material layer.
The beneficial effect of this novel anaerobic bacteria cultivation bucket is:
the utility model discloses a set up heating mechanism, temperature sensor, gas pressure sensor, gas concentration sensor, controller, temperature, gas pressure, the gas concentration in the rational control staving to provide good culture environment for anaerobe, be favorable to improving cultivation efficiency and cultivation quality, simultaneously, the utility model discloses a rationally set up annular bracket, under the circumstances of make full use of staving inner space, can make the experimenter in time observe the culture state of anaerobe in the culture dish.
Drawings
FIG. 1, a schematic cross-sectional view of the present new;
FIG. 2 is a schematic cross-sectional view of the novel A-A;
1. a base; 2. a double glass sidewall; 2-1, outer layer glass; 2-2, inner layer glass; 3. a thermal insulation board; 4. a sleeve; 5. a heat-permeable hole; 6. a heating rod; 7. a culture dish; 8. an annular side plate; 9. an annular bottom plate; 10. sealing cover; 11. an air inlet pipe; 12. an exhaust pipe; 13. a controller; 14. and a limit groove.
Detailed Description
The following description is of the preferred embodiments of the present utility model and is not intended to limit the scope of the utility model, but is intended to cover any modifications, equivalents, improvements, etc. within the spirit and principles of the present utility model.
In this embodiment, an anaerobic bacteria culture barrel, as shown in fig. 1 and 2, the anaerobic bacteria culture barrel comprises a barrel body, a sealing cover 10 and a controller 13, wherein the barrel body is provided with a double-layer glass side wall 2, a heating mechanism and a temperature sensor (not labeled in the figure) are arranged in the barrel body, the controller 13 is arranged outside the barrel body and is electrically connected with a power supply, the temperature sensor and the heating mechanism respectively through wires, a plurality of mutually overlapped annular brackets are arranged in the barrel body, each annular bracket comprises an annular bottom plate 9 which is horizontally arranged, an annular side plate 8 which is arranged at the outer edge of the upper surface of the annular bottom plate 9 and extends upwards in the longitudinal direction, a plurality of limiting grooves 14 are uniformly distributed on the upper surface of the annular bottom plate 9, the limiting grooves 14 are used for placing transparent culture dishes 7, the top end of the annular side plate 8 is propped against the outer edge of the lower surface of the annular bottom plate 9 adjacent to the upper side, the annular side plate 8 is made of transparent materials, and the height of the annular side plate 8 is as follows: the culture form of anaerobic bacteria in the culture dish can be seen through the double-layer glass side wall 2.
In this embodiment, as shown in fig. 1 and 2, the side wall 2 of the double-layer glass has a cylindrical structure, and includes an inner layer glass 2-2 positioned in an inner layer and an outer layer glass 2-1 positioned in an outer layer, vacuum pumping is performed between the inner layer glass and the outer layer glass, top ends of the inner layer glass and the outer layer glass are fixedly connected with a fixing ring (not labeled in the figure), an inner surface of the fixing ring is coplanar with an inner surface of the inner layer glass 2-2, the fixing ring is provided with an external thread, and the external thread is screwed with the sealing cover 10; the bottom of the double-layer glass side wall 2 is connected with a base 1.
In this embodiment, as shown in fig. 1 and 2, the heating mechanism includes a sleeve 4 coaxially and fixedly connected to the bottom end of the sealing cover 10, a heating rod 6 is coaxially disposed in the sleeve 4, the top end of the heating rod 6 is fixedly connected to the bottom end of the sealing cover 10 through a connecting seat, and a plurality of diathermic holes 5 are uniformly distributed on the side wall of the sleeve 4.
In this embodiment, as shown in fig. 1 and 2, the controller 13 is disposed at the center of the top end of the sealing cover 10 and is electrically connected to the heating rod 6 through a wire, and the inner edge of the annular bottom plate 9 is in clearance fit with the outer surface of the sleeve 4.
In this embodiment, as shown in fig. 1 and 2, the sealing cover 10 further penetrates through an air inlet pipe 11 and an air outlet pipe 12, and adjusting valves (not labeled in the drawings) are respectively arranged on pipe bodies of the air inlet pipe 11 and the air outlet pipe 12 above the sealing cover 10.
In this embodiment, as shown in fig. 1 and 2, a temperature sensor (not labeled in the drawing) and a gas concentration sensor (not labeled in the drawing, for detecting the concentration of the anaerobic bacteria suitable for the gas) are disposed at the bottom end of the sealing cover 10, and the gas concentration sensor is electrically connected with the controller 13 through a wire.
In this embodiment, as shown in fig. 1 and 2, a gas pressure sensor (not labeled in the drawing) is further disposed at the bottom end of the sealing cover 10, and the gas pressure sensor is electrically connected to the controller 13 through a wire. The purpose of setting up gas pressure sensor is to detect the gas pressure in the staving, and the gas pressure in the adjustable staving of through the governing valve on the blast pipe is located invariable scope.
In this embodiment, as shown in fig. 1 and 2, the upper surface of the base is further provided with an insulation board 3, the upper surface of the insulation board 3 is attached to the lower surface of the annular bottom board at the bottommost layer, and the sealing cover is provided with an insulation material layer (not shown in the figure, and is of a common structure).
The utility model discloses during the use, open sealed lid, with each annular bracket that puts the culture dish in proper order stack in the staving, then insert the sleeve, screw sealed lid, connect the power, connect evacuating device through the blast pipe, with the interior evacuation of staving, then through the gas that the intake pipe input is fit for anaerobic bacteria and cultivate, detect gas concentration through gas concentration sensor, detect gas pressure through gas pressure sensor, in this process, if surpass concentration and the pressure of settlement, the controller is through the appropriate partial gas of discharging of governing valve of control blast pipe, when reaching the gas concentration and the gas pressure of settlement, 2 governing valves close. In the culture process, the temperature in the barrel body is raised through the heating rod, heating is stopped after the temperature reaches the set temperature, and the barrel body is heated again after the temperature is lower than the set temperature, so that the temperature in the barrel body is kept constant. After the anaerobic bacteria in the culture dish are observed to be cultured, the sealing cover can be opened, and the culture dish is taken out in sequence.
Claims (8)
1. An anaerobic bacteria culture barrel is characterized in that: including staving, sealed lid, controller, the staving be equipped with double glazing lateral wall, be equipped with heating mechanism and temperature sensor in the staving, the controller locate the staving outside and be connected with power, temperature sensor, heating mechanism electricity respectively through the wire, the staving in be equipped with a plurality of mutual superimposed annular brackets, annular bracket include along the annular bottom plate that the level set up, locate annular bottom plate upper surface outer fringe and along the annular curb plate of vertically upwards extending, the surface and the staving internal surface clearance fit of annular curb plate, evenly distributed have a plurality of spacing grooves on annular bottom plate upper surface, the spacing groove be used for putting transparent culture dish, the top of annular curb plate offset with the lower surface outer fringe of the adjacent annular bottom plate of top, annular curb plate be transparent material and make, the height of annular curb plate satisfies: the culture form of anaerobic bacteria in the culture dish can be seen through the double-layer glass side wall.
2. An anaerobic bacteria cultivation vat according to claim 1, wherein: the double-layer glass side wall is of a cylindrical structure and comprises inner-layer glass positioned at an inner layer and outer-layer glass positioned at an outer layer, vacuumizing treatment is carried out between the inner-layer glass and the outer-layer glass, the top ends of the inner-layer glass and the outer-layer glass are fixedly connected with a fixing ring together, the inner surface of the fixing ring is coplanar with the inner surface of the inner-layer glass, the fixing ring is provided with external threads, and the external threads are in threaded connection with a sealing cover; the bottom of the side wall of the double-layer glass is connected with a base.
3. An anaerobic bacteria cultivation vat according to claim 2, wherein: the heating mechanism comprises a sleeve which is coaxially and fixedly connected to the bottom end of the sealing cover, a heating rod is coaxially arranged in the sleeve, the top end of the heating rod is fixedly connected with the bottom end of the sealing cover through a connecting seat, and a plurality of heat penetrating holes are uniformly distributed on the side wall of the sleeve.
4. An anaerobic bacteria culture vat according to claim 3, wherein: the controller is arranged at the center of the top end of the sealing cover and is electrically connected with the heating rod through a wire, and the inner edge of the annular bottom plate is in clearance fit with the outer surface of the sleeve.
5. An anaerobic bacteria cultivation vat according to claim 4, wherein: the sealing cover also penetrates through the air inlet pipe and the air outlet pipe, and the air inlet pipe and the air outlet pipe are respectively provided with a regulating valve on the pipe body above the sealing cover.
6. An anaerobic bacteria cultivation vat according to claim 5, wherein: the bottom end of the sealing cover is provided with a temperature sensor and a gas concentration sensor, and the gas concentration sensor is electrically connected with the controller through a wire.
7. An anaerobic culture vat according to claim 6, wherein: the bottom end of the sealing cover is also provided with a gas pressure sensor, and the gas pressure sensor is electrically connected with the controller through a wire.
8. An anaerobic bacteria cultivation vat according to claim 7, wherein: the base upper surface still be equipped with the heated board, heated board upper surface and the laminating of the annular bottom plate lower surface of bottommost, sealed lid be equipped with the heat preservation material layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321803140.3U CN220317818U (en) | 2023-07-11 | 2023-07-11 | Anaerobic bacteria cultivates bucket |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321803140.3U CN220317818U (en) | 2023-07-11 | 2023-07-11 | Anaerobic bacteria cultivates bucket |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220317818U true CN220317818U (en) | 2024-01-09 |
Family
ID=89412066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321803140.3U Active CN220317818U (en) | 2023-07-11 | 2023-07-11 | Anaerobic bacteria cultivates bucket |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220317818U (en) |
-
2023
- 2023-07-11 CN CN202321803140.3U patent/CN220317818U/en active Active
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