CN217809433U - Optical system of PCR (polymerase chain reaction) fluorescence detector and PCR fluorescence detector - Google Patents
Optical system of PCR (polymerase chain reaction) fluorescence detector and PCR fluorescence detector Download PDFInfo
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- 230000003287 optical effect Effects 0.000 title claims abstract description 69
- 238000003752 polymerase chain reaction Methods 0.000 title description 53
- 238000001514 detection method Methods 0.000 claims abstract description 61
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- 230000005284 excitation Effects 0.000 claims description 10
- 239000000758 substrate Substances 0.000 claims description 10
- 239000000975 dye Substances 0.000 description 11
- 239000000463 material Substances 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 3
- 230000004543 DNA replication Effects 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 150000007523 nucleic acids Chemical group 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 239000012099 Alexa Fluor family Substances 0.000 description 1
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 1
- 229910005540 GaP Inorganic materials 0.000 description 1
- 229910052581 Si3N4 Inorganic materials 0.000 description 1
- IVHDZUFNZLETBM-IWSIBTJSSA-N acridine red 3B Chemical compound [Cl-].C1=C\C(=[NH+]/C)C=C2OC3=CC(NC)=CC=C3C=C21 IVHDZUFNZLETBM-IWSIBTJSSA-N 0.000 description 1
- 229910021417 amorphous silicon Inorganic materials 0.000 description 1
- 239000005331 crown glasses (windows) Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000005308 flint glass Substances 0.000 description 1
- HZXMRANICFIONG-UHFFFAOYSA-N gallium phosphide Chemical compound [Ga]#P HZXMRANICFIONG-UHFFFAOYSA-N 0.000 description 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
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Abstract
The utility model provides an optical system of a PCR fluorescence detector and the PCR fluorescence detector, at least two lasers, collimation super lens, transflective element, detection sample and a plurality of receiving elements are arranged in the optical system of the PCR fluorescence detector, and because the collimation super lens is used, the optical element matched with each laser is not required to be arranged, thereby greatly reducing the complexity of the optical system used by the PCR detector; moreover, when a laser is required to be added to emit light with a new wavelength to excite a detection sample, only one laser which emits light with different wavelengths is required to be added to one side of the collimating metalens, and an optical element matched with the newly added laser is not required to be designed, so that the complexity of an optical system used by the PCR detector is reduced.
Description
Technical Field
The utility model relates to a super application of surface field particularly, relates to an optical system and PCR fluorescence detector of Polymerase Chain Reaction (PCR) fluorescence detector.
Background
Currently, PCR is a nucleic acid amplification technique. The technology simulates the natural DNA replication process in vitro, improves the DNA concentration in the detection liquid, and obtains the concentration of micro-droplets containing specific DNA through dye dyeing, fluorescence excitation, fluorescence signal detection and analysis, thereby realizing the detection function of related substances.
The existing PCR detector adopts a confocal optical system, wherein different light sources respectively form a plurality of sets of excitation sources with matched optical elements, and emit a plurality of excitation lights with stable intensity to enter a sample, so that the sample dyed by a dye is excited to generate a plurality of fluorescence, and a plurality of sets of different receiving systems are utilized to receive the fluorescence with different wavelengths in the plurality of fluorescence, thereby leading the optical system used by the PCR detector to be very complex.
SUMMERY OF THE UTILITY MODEL
In order to solve the above problem, an object of the embodiments of the present invention is to provide an optical system of a PCR fluorescence detector and a PCR fluorescence detector.
In a first aspect, an embodiment of the present invention provides an optical system of a PCR fluorescence detector, for exciting a detection sample to emit fluorescence, including: at least two lasers, a collimating metalens, a transflector element, and a plurality of receiving elements;
each laser in the at least two lasers respectively emits light rays with different wavelengths;
the collimating super lens collimates each light ray with different wavelength emitted by each laser, and each light ray with different wavelength collimated by the collimating super lens is incident to the transflective element;
the transflective element reflects each incident light ray with different wavelength, irradiates each light ray with different wavelength onto the detection sample, and excites the detection sample, so that the excited detection sample emits a plurality of fluorescence to the transflective element;
the transflective element transmits a plurality of fluorescent lights so that the plurality of fluorescent lights are respectively incident on each receiving element of the plurality of receiving elements;
each of the receiving elements receives different fluorescence from among the plurality of fluorescence.
In a second aspect, the embodiments of the present invention further provide a PCR fluorescence detector, including: the optical system of the PCR fluorescence detector according to the first aspect.
In the embodiment of the present invention, in the scheme provided by the above-mentioned first aspect to the second aspect, through setting up at least two lasers, collimation super lens, transflective element, detection sample and a plurality of receiving elements in the optical system of the PCR fluorescence detector, the light with different wavelengths respectively emitted by each laser in at least two lasers is collimated through the collimation super lens, the light with different wavelengths is incident on the transflective element by the plurality of collimated lights of the collimation super lens, the light with different wavelengths is irradiated on the detection sample by the plurality of reflected lights of the transflective element, the detection sample is excited, so that the detection sample after being excited emits a plurality of fluorescence to the transflective element; the transflective element transmits a plurality of fluorescent lights to enable the plurality of fluorescent lights to be respectively incident on each receiving element of the plurality of receiving elements, and compared with a PCR detector adopting a confocal optical system in the related art, the collimating super lens is used, so that an optical element matched with each laser is not required to be arranged, and the complexity of the optical system used by the PCR detector is greatly reduced; moreover, when a laser is required to be added to emit light with a new wavelength to excite a detection sample, only one laser is required to be added on one side of the collimating metalens, and an optical element matched with the newly added laser is not required to be designed, so that the use complexity of an optical system used by the PCR detector is reduced, and the reusability of the optical system used by the PCR detector is increased.
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram illustrating an optical system of a PCR fluorescence detector according to an embodiment of the present invention;
fig. 2 is a schematic diagram illustrating a periodic arrangement of nanostructures on a substrate in an optical system of a PCR fluorescence detector provided in an embodiment of the present invention;
fig. 3 is a schematic diagram illustrating a sample spot in a light path represented by light emitted by a simulated laser in an optical system of a PCR fluorescence detector provided in an embodiment of the present invention;
fig. 4 is a schematic diagram illustrating a light spot of fluorescence having a second characteristic received at a simulated receiving element in an optical system of a PCR fluorescence detector provided in an embodiment of the present invention.
Detailed Description
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Currently, PCR is a nucleic acid amplification technique. The technology simulates the natural DNA replication process in vitro, improves the DNA concentration in the detection liquid, and obtains the concentration of micro-droplets containing specific DNA through dye dyeing, fluorescence excitation, fluorescence signal detection and analysis, thereby realizing the detection function of related substances.
The existing PCR detector adopts a confocal optical system, wherein different light sources respectively form a plurality of sets of excitation sources with matched optical elements, and a plurality of excitation light beams are emitted at stable intensity and enter a sample, so that the sample dyed by the dye is excited to generate a plurality of fluorescence, and a plurality of sets of different receiving systems are used for receiving the fluorescence with different wavelengths in the plurality of fluorescence, thereby leading the optical system used by the PCR detector to be very complex.
Based on this, this embodiment provides an optical system of a PCR fluorescence detector and a PCR fluorescence detector, in which at least two lasers, a collimating metalens, a transflective element, a detection sample and a plurality of receiving elements are disposed in the optical system of the PCR fluorescence detector, a plurality of lights with different wavelengths respectively emitted by each of the at least two lasers are collimated by the collimating metalens, the plurality of lights with different wavelengths collimated by the collimating metalens are incident on the transflective element, the plurality of lights with different wavelengths reflected by the transflective element are irradiated onto the detection sample, and the detection sample is excited, so that the detection sample after being excited emits a plurality of fluorescence with a second characteristic to the transflective element; the transflective element transmits the plurality of fluorescent lights with the second characteristic, so that the plurality of fluorescent lights with the second characteristic respectively enter each receiving element of the plurality of receiving elements, and due to the fact that the collimating super lens is used, an optical element matched with each laser is not needed to be arranged, and complexity of an optical system used by the PCR detector is greatly reduced.
In order to make the aforementioned objects, features and advantages of the present application more comprehensible, the present application is described in further detail with reference to the accompanying drawings and the detailed description.
Example 1
Referring to fig. 1, a schematic structural diagram of an optical system of a PCR fluorescence detector is provided, in this embodiment, an optical system of a PCR fluorescence detector is provided, for exciting a detection sample 106 to emit fluorescence, and includes: at least two lasers 100, a collimating metalens 102, a transflector element 104 and a plurality of receiving elements 108.
In one embodiment, the receiving element is a photodiode.
Each of the at least two lasers emits light having a different wavelength.
The collimating super lens collimates a plurality of light rays with different wavelengths respectively emitted by the lasers, and the plurality of light rays with different wavelengths collimated by the collimating super lens are incident to the transflective element.
Optionally, in order to make the collimating metalens better collimate the light emitted by each laser with different wavelengths, the lasers are located on the focal plane of the collimating metalens.
The transflective element reflects the incident light rays with different wavelengths, irradiates the light rays with different wavelengths onto the detection sample, and excites the detection sample, so that the excited detection sample emits a plurality of fluorescence with second characteristics to the transflective element.
The test sample is coated with a plurality of dyes. For different dyes in a plurality of dyes, light rays with different wavelengths are required to be irradiated for excitation so as to emit different fluorescence. The different fluorescent light has different wavelengths.
In one embodiment, the first fluorescent dye coated on the sample is detected, and may be selected from Acridine Red (ethanol), which has an excitation wavelength of 552 nanometers (nm) and an emission wavelength of 584nm; the second dye coated on the detection sample is Alexa Fluor fluorescent dye, the excitation wavelength of the second dye is 561nm, and the emission wavelength of the second dye is 572nm.
Therefore, for the optical system of the twin laser of the PCR fluorescence detector, a first laser can be provided for emitting light with a wavelength of 552nm to irradiate the detection sample, so as to excite the detection sample to emit fluorescence with a wavelength of 584nm; a second laser can be arranged for emitting light with the wavelength of 561nm to irradiate the detection sample, so that the detection sample is excited to emit fluorescence with the wavelength of 572nm.
The transflective element transmits the plurality of fluorescent lights having the second characteristic, so that the plurality of fluorescent lights having the second characteristic are incident on each of the plurality of receiving elements.
The transflective element is a beam splitter.
The receiving elements receive only the fluorescence having the same second characteristic.
In order to collimate incident light, in the optical system of the PCR fluorescence detector provided in this embodiment, the modulation phase of the collimating super lens when collimating light satisfies the public
Formula 1:
wherein,
the modulation phase of the collimation superlens when collimating the light emitted by the ith laser in the at least two lasers is represented;
representing the wave front phase distribution of the light emitted by the laser when the light propagates to the surface of the collimating superlens; r is 1 The distance from any point on the collimating super lens to the center of the collimating super lens is represented; theta i Representing the angle between the optical axis of the ith laser and the normal of the collimating superlens.
The sizes of the parameters appearing on the right side of the middle number in the above formula 1 are all preset numerical values.
If the optical system of the PCR fluorescence detector is the optical system of the twin laser, then i =1 and 2; theta 1 ＝30°；θ 2 ＝0°。
θ 1 By =30 ° is meant that the angle between the optical axis of the 1 st laser and the normal of the collimating superlens is 30 degrees.
θ 2 =0 ° indicates that the angle between the optical axis of the 1 st laser and the normal of the collimating superlens is 0 degrees.
Further, in order to make the plurality of light beams with different wavelengths reflected by the transflective element converge on the surface of the detection sample well, the optical system of the PCR fluorescence detector provided in this embodiment further includes: a focusing superlens 110.
The focusing super lens is arranged between the transflective element and the detection sample.
In order to better converge the plurality of light rays with different wavelengths reflected by the transflective element to the surface of the detection sample, the detection sample may be disposed on the focal plane of the focusing superlens.
The focusing super lens can converge the light rays with different wavelengths reflected by the transflective element, and the converged light rays with different wavelengths are incident on the detection sample to excite the detection sample.
The step of exciting the detection sample refers to exciting a dye coated on the surface of the detection sample.
In order to converge the incident light, in the optical system of the PCR fluorescence detector provided in this embodiment, the adjustment phase of the focusing superlens when the light converges satisfies the following formula 2:
wherein,
the modulation phase of the focusing super lens when converging the light emitted by the ith laser is represented; lambda [ alpha ] i Represents the wavelength of the light emitted by the ith laser; r is a radical of hydrogen 2 The distance from any point on the focusing super lens to the center of the focusing super lens is represented; f. of 2 Indicating the focal length of the focusing superlens.
The sizes of the parameters appearing on the right side of the middle mark in the above formula 2 are all preset numerical values.
When the receiving elements receive only fluorescence having the same wavelength, the description will be given of the case where each receiving element receives only fluorescence having a single wavelength. In order to better feed back the fluorescence with a single wavelength to the receiving element for receiving the fluorescence, the optical system of the PCR fluorescence detector proposed in this embodiment further includes: a beam splitting superlens 112.
The beam splitting superlens is arranged between the transflective element and each receiving element in the plurality of receiving elements.
In order to accurately feed back the fluorescence of a single wavelength to the receiving elements receiving the fluorescence, each receiving element may be disposed on the focal plane of the beam splitting superlens.
The beam splitting superlens can perform phase modulation on the different fluorescent lights transmitted by the transflective element, and converge each fluorescent light with different wavelengths in the different fluorescent lights to a corresponding receiving element at a specific angle according to the wavelength.
The fluorescence is converged to a corresponding receiving element at a specific angle according to the wavelength, namely the result of phase modulation on the fluorescence by the beam splitting superlens according to the wavelength of the fluorescence.
Specifically, the modulation phase of the spectroscopic superlens for splitting fluorescence light satisfies formula 3:
wherein,
the phase position of the fluorescence emitted by the detection sample after being excited by the light emitted by the ith laser is modulated by the light splitting super lens; k is a radical of 0 Represents the wave number in vacuum; r is 3 The distance from any point on the beam splitting super lens to the center of the focusing super lens is represented; f. of 3 Represents the focal length of the beam splitting superlens; theta.theta. si Indicating the exit angle of the detection sample after the fluorescence emitted by the ith laser after being excited by the light emitted by the ith laser is split.
If the optical system of the PCR fluorescence detector is the optical system of the twin laser, then i =1 and 2; then, theta s1 ＝30°；θ s2 ＝0°。
θ s1 The number of the detection samples is =30 ° and represents fluorescence emitted after the detection samples are excited by light emitted by the 1 st laser, and the exit angle after the detection samples are split by the splitting superlens is 30 °.
θ s2 Table of =0 degThe emission angle of the fluorescence emitted by the sample after being excited by the light emitted by the 2 nd laser after being split by the splitting superlens is 0 degree.
The sizes of the parameters appearing on the right side of the middle number in the above formula 3 are all preset numerical values.
The collimating super lens, the focusing super lens and the beam splitting super lens respectively comprise: a substrate and a nanostructure disposed on the substrate.
The substrate is a visible light wave band transparent material; preferably, the substrate materials, include, but are not limited to: quartz glass, crown glass, flint glass. The working wave bands of the collimating super lens, the focusing super lens and the light splitting super lens are visible light (the wavelength of the visible light is 380nm to 760 nm).
Referring to the schematic diagram of the arrangement of the nanostructures on the substrate according to the period shown in fig. 2, optionally, the arrangement period of the nanostructures on the substrate is 200nm to 1500nm; the periodic center or apex has nanostructures. The nanostructure material is an application band transparent material, and the optional materials include but are not limited to: titanium oxide, silicon nitride, gallium phosphide, aluminum oxide, hydrogenated amorphous silicon. The nano-structures can be filled with air or other materials transparent in the application wave band, and it should be noted that the absolute value of the difference between the refractive index of the filling material between the nano-materials and the refractive index of the nano-structures is greater than or equal to 0.5.
The nanostructure is a polarization independent structure, such as a nanocylinder and a nanocylinder, which applies a propagation phase to incident light, thereby modulating the phase of the incident light.
In one embodiment, if the optical system of the PCR fluorescence detector is the optical system of the dual laser, the collimating metalens, the focusing metalens, and the beam splitting metalens are designed and selected as follows: selecting a nanometer cylinder as the nanometer structure; the material of the nano structure is titanium dioxide, and the material selected for the substrate is silicon dioxide. The period of the nano cylinder is 450nm, and the height of the nano cylinder is 1200nm. Focal length f of light splitting super lens 2 And focal length f of the focusing superlens 3 Are set to 500 μm.
The optical system of the PCR fluorescence detector provided by the embodiment of the application designs the angle/wavelength multiplexing super lens, can integrate the collimation light path and the fluorescence collection light path of multiple paths of light rays onto one super lens respectively, and has the advantages of simple system, miniaturization, light weight, low cost and the like.
The embodiment also provides a PCR fluorescence detector, which uses the optical system of the PCR fluorescence detector. Referring to the sample light spot in the light path shown by the light emitted by the simulated laser shown in fig. 3 and the light spot of the fluorescence with the second characteristic received by the simulated receiving element shown in fig. 4, it can be seen that the light spot of the fluorescence detected by the receiving element can substantially reduce the light emitted by the detection sample, the collimation degree of the fluorescence is high, and the energy distribution of the fluorescence received by the receiving element is concentrated and uniform, so that it can be ensured that the input signal obtained by photoelectrically converting the fluorescence by the receiving element is very stable, and the input signal transmitted to the detection circuit connected to the receiving element is very stable, so that the detection circuit can obtain a stable detection result for the detection sample.
In summary, the present embodiment provides an optical system of a PCR fluorescence detector and a PCR fluorescence detector, in which at least two lasers, a collimating metalens, a transflective element, a detection sample and a plurality of receiving elements are disposed in the optical system of the PCR fluorescence detector, a plurality of lights with different wavelengths respectively emitted by each of the at least two lasers are collimated by the collimating metalens, the plurality of lights with different wavelengths collimated by the collimating metalens are incident on the transflective element, the plurality of lights with different wavelengths reflected by the transflective element are irradiated onto the detection sample, and the detection sample is excited, so that the excited detection sample emits a plurality of fluorescence to the transflective element; the transflective element transmits a plurality of fluorescent lights to enable the plurality of fluorescent lights to respectively enter each receiving element of the plurality of receiving elements, and compared with a PCR detector adopting a confocal optical system in the related art, due to the fact that the collimating superlens is used, an optical element matched with each laser is not needed to be arranged, and complexity of the optical system used by the PCR detector is greatly reduced; moreover, when a laser is required to be added to emit light with a new wavelength to excite a detection sample, only one laser is required to be added on one side of the collimating metalens, and an optical element matched with the newly added laser is not required to be designed, so that the use complexity of an optical system used by the PCR detector is reduced, and the reusability of the optical system used by the PCR detector is increased.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present invention, and all should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (9)
1. An optical system of a PCR fluorescence detector for exciting a detection sample to emit fluorescence, comprising: at least two lasers, a collimating metalens, a transflector element, and a plurality of receiving elements;
each laser in the at least two lasers respectively emits light rays with different wavelengths;
the collimating super lens collimates each light ray with different wavelength emitted by each laser, and each light ray with different wavelength collimated by the collimating super lens is incident to the transflective element;
the transflective element reflects each incident light ray with different wavelength, irradiates each light ray with different wavelength on the detection sample, and excites the detection sample, so that the excited detection sample emits a plurality of fluorescence to the transflective element;
the transflective element transmits a plurality of fluorescent lights so that the plurality of fluorescent lights are respectively incident on each receiving element of the plurality of receiving elements;
each of the receiving elements receives different fluorescence from among the plurality of fluorescence.
2. The optical system of the PCR fluorescence detector of claim 1, wherein the different fluorescence has different wavelengths.
3. The optical system of the PCR fluorescence detector of claim 2, wherein the modulation phase of the collimating metalens when collimating the light satisfies the following formula:
wherein,
the modulation phase of the collimation superlens when collimating the light emitted by the ith laser in the at least two lasers is represented;
representing the wave front phase distribution of the light emitted by the laser when the light propagates to the surface of the collimating superlens; r is 1 The distance from any point on the collimating super lens to the center of the collimating super lens is represented; theta.theta. i Representing the angle between the optical axis of the ith laser and the normal of the collimating superlens.
4. The optical system of the PCR fluorescence detector of claim 2, further comprising: a focusing superlens;
the focusing super lens is arranged between the transflective element and the detection sample;
the focusing super lens can converge the light rays with different wavelengths reflected by the transflective element, and the converged light rays with different wavelengths are incident on the detection sample to excite the detection sample.
5. The optical system of the PCR fluorescence detector of claim 4, wherein the modulation phase when the focusing super lens converges the light satisfies the following formula:
wherein,
the modulation phase of the focusing super lens when converging the light emitted by the ith laser is represented; lambda [ alpha ] i Represents the wavelength of the light emitted by the ith laser; r is 2 The distance from any point on the focusing super lens to the center of the focusing super lens is represented; f. of 2 Indicating the focal length of the focusing superlens.
6. The optical system of the PCR fluorescence detector of claim 4, further comprising: a beam splitting superlens;
the beam splitting super lens is arranged between the transflective element and each receiving element in the plurality of receiving elements;
the beam splitting super lens can perform phase modulation on the different fluorescent lights transmitted by the transflective element, and converge each fluorescent light with different wavelengths in the different fluorescent lights onto a corresponding receiving element at a specific angle according to the wavelength;
the modulation phase of the light splitting super lens for light splitting of the fluorescence meets the following formula:
wherein,
indicating fluorescence emitted by the sample being examined after excitation by light emitted by the ith laserThe phase of the light modulated by the beam splitting superlens; k is a radical of 0 Represents the wave number in vacuum; r is 3 The distance from any point on the beam splitting super lens to the center of the focusing super lens is represented; f. of 3 Represents the focal length of the beam splitting superlens; theta si Indicating the exit angle of the detection sample after the fluorescence emitted by the ith laser after being excited by the light emitted by the ith laser is split.
7. The optical system of the PCR fluorescence detector of claim 6, wherein the collimating metalens, the focusing metalens, and the beam splitting metalens respectively comprise: a substrate and a nanostructure disposed on the substrate.
8. The optical system of the PCR fluorescence detector of claim 1, wherein the receiving element is a photodiode.
9. A PCR fluorescence detector, comprising: the optical system of the PCR fluorescence detector according to any one of claims 1 to 8.
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Cited By (3)
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| US11927769B2 (en) | 2022-03-31 | 2024-03-12 | Metalenz, Inc. | Polarization sorting metasurface microlens array device |
| US11978752B2 (en) | 2019-07-26 | 2024-05-07 | Metalenz, Inc. | Aperture-metasurface and hybrid refractive-metasurface imaging systems |
| US11988844B2 (en) | 2017-08-31 | 2024-05-21 | Metalenz, Inc. | Transmissive metasurface lens integration |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11988844B2 (en) | 2017-08-31 | 2024-05-21 | Metalenz, Inc. | Transmissive metasurface lens integration |
| US11978752B2 (en) | 2019-07-26 | 2024-05-07 | Metalenz, Inc. | Aperture-metasurface and hybrid refractive-metasurface imaging systems |
| US11927769B2 (en) | 2022-03-31 | 2024-03-12 | Metalenz, Inc. | Polarization sorting metasurface microlens array device |
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