CN206740772U - A kind of whole blood is quantitatively loaded the kit of detection cardiac muscle three - Google Patents
A kind of whole blood is quantitatively loaded the kit of detection cardiac muscle three Download PDFInfo
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- CN206740772U CN206740772U CN201720566988.7U CN201720566988U CN206740772U CN 206740772 U CN206740772 U CN 206740772U CN 201720566988 U CN201720566988 U CN 201720566988U CN 206740772 U CN206740772 U CN 206740772U
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Abstract
The utility model provides the kit that a kind of whole blood is quantitatively loaded detection cardiac muscle three, including the lid that gets stuck, bottom of getting stuck, and the described lid that gets stuck is positioned at the top at described bottom of getting stuck；It is described get stuck to cover be provided with whole blood loading slot, the bottom of described whole blood loading slot is provided with sample holes, and the side wall of described whole blood loading slot is provided with some excessive sample holes, and the side of described whole blood loading slot is provided with dilution well；The bottom at described bottom of getting stuck is provided with reagent strip screens.The kit that whole blood described in the utility model is quantitatively loaded detection cardiac muscle three is provided with excessive sample hole, water conservancy diversion track, overflow sample groove, the addition volume of blood or whole blood crosses conference and result is interfered between finger, the addition of whole blood is automatically controlled by the setting in sample hole of overflowing, water conservancy diversion track, the sample groove that overflows, remaining whole blood then flows into excessive sample groove, is normally detected so as to not influence reagent strip.
Description
Technical field
The utility model belongs to medical instruments field, and the examination of detection cardiac muscle three is quantitatively loaded more particularly, to a kind of whole blood
Agent box.
Background technology
Cardiovascular and cerebrovascular disease endangers the master of our people's health as our people's growth in the living standard turns into
Reason is wanted, and cardiovascular and cerebrovascular disease and its sequelae cause the decline of people's living standard indirectly.If myocardial infarction is in disease
Shape occur after preceding 1-2 hours in handle in time and correctly, it is possible to make patient out of danger.It is common as the elderly
A kind of acute disease, will be significant to reducing case fatality rate if can accomplish to early diagnose.
Immune colloidal gold technique, mainly by collaurum, latex or other corresponding mass signatures antibody (original), then will
Corresponding antigen (body), which is coated in chromatographic film, forms detecting system.During detection, the grain dissolution of mark is passed through chromatography by sample
Act on film and move, and be coated on antigen on film (body) capture, form detection line, testing result can pass through naked eyes or corresponding
Instrument result of determination.
The domestic and international confirmation method of acute myocardial infarction mainly utilizes characteristic ECG change and laboratory examination hand
Section.Currently as general detection albumen in the world laboratory techniques mainly comprising EUSA (ELISA) and
Radioimmunoassays (RIA).Radioimmunoassays specificity is good, high sensitivity is currently generally acknowledged confirmation experimental method.But its
Repeatability is poor, and non-specific binding rate is higher, and has the harm of radioisotope pollution.And ELISA method is time-consuming longer,
And two methods are required to technical professional and operated.And immune colloidal gold method is due to its outstanding technical characterstic,
Approved extensively by World Medical mechanism.This collaurum project can be used for Clinical screening miocardial infarction disease, fundamentally carry
The recall rate of high miocardial infarction disease, epidemic disease treatment level is improved, the mesh for realize the generaI investigation of major disease basic unit, preventing trouble before it happens
's.
Immune detection colloidal gold strip is a kind of quick, easily detection method, particularly suitable for Site Detection, detection
Sample can be urine, excrement, serum, blood plasma and whole blood etc..Wherein serum and blood plasma are required for after handling whole blood,
It can be tested, manufacturer develops the reagent strip suitable for whole blood at present, in order to preferably adapt to the demand of Site Detection,
It is badly in need of kit of the exploitation with corresponding function.Only need to puncture finger with sterilization needle set in detecting at the scene, it is complete to take out 1 drop
Blood, it is added drop-wise in kit, but during blood between pricking fetching, due to the limitation of various factors, may takes out
Blood flow volume is different, the inconsistent of blood sample addition is thus caused, so as to have impact on the accuracy of testing result.
The content of the invention
In view of this, the utility model is directed to the kit that a kind of whole blood is quantitatively loaded detection cardiac muscle three, operation
Simply, it is easy to use to be quantitatively loaded with whole blood.
To reach above-mentioned purpose, what the technical solution of the utility model was realized in：
A kind of whole blood is quantitatively loaded the kit of detection cardiac muscle three, including the lid that gets stuck, bottom of getting stuck, described lid position of getting stuck
In the top at described bottom of getting stuck；
It is described get stuck to cover be provided with whole blood loading slot, the bottom of described whole blood loading slot is provided with sample holes, described
The side wall of whole blood loading slot be provided with some excessive sample holes, the side of described whole blood loading slot is provided with dilution well；
The bottom at described bottom of getting stuck is provided with reagent strip screens, water conservancy diversion track is provided with described bottom of getting stuck, overflow sample
Groove, guide ring, described excessive sample groove are fixed in the side wall at described bottom of getting stuck, one end of described water conservancy diversion track and excessive sample groove
Connect, the other end is fixed on described guide ring, and described guide ring is located at the lower section of described sample holes.
Further, described water conservancy diversion track inclination is set, and the upper end of described water conservancy diversion track is connected with described guide ring,
Lower end is connected with described excessive sample groove.
Further, the external diameter of the bottom of described whole blood loading slot is more than or equal to the external diameter of described guide ring.
Further, it is described get stuck to cover be provided with form, described form is located at the side of described whole blood loading slot.
Further, described form is located at the top of described reagent strip screens.
The kit that described whole blood is quantitatively loaded detection cardiac muscle three is applied to myocardium three (cTnI, CK-MB, MYO),
Cardiac troponin (cTnI), creatine kinase isozyme (CK-MB), myoglobins (Myo) etc. can be with the reagent strips of whole blood test.
Relative to prior art, whole blood described in the utility model be quantitatively loaded detection cardiac muscle three kit have with
Lower advantage：
(1) whole blood described in the utility model is quantitatively loaded myocardium three kit of detection and is provided with excessive sample hole, water conservancy diversion
Track, overflow sample groove, and the addition volume for referring to a blood or whole blood crosses conference result is interfered, and by sample hole of overflowing, water conservancy diversion track, overflows
The setting of sample groove automatically controls the addition of whole blood, and remaining whole blood then flows into excessive sample groove, is normally detected so as to not influence reagent strip.
(2) whole blood described in the utility model, which is quantitatively loaded myocardium three kit of detection, can make to be applied to low dose entirely
Blood examination is surveyed, and simple to operate, easy to use, can be quantitatively loaded with whole blood.
Brief description of the drawings
Form a part of accompanying drawing of the present utility model to be used for providing further understanding to of the present utility model, this practicality is new
The schematic description and description of type is used to explain the utility model, does not form to improper restriction of the present utility model.
In accompanying drawing：
Fig. 1 is the schematic diagram of the lid that gets stuck described in the utility model embodiment；
Fig. 2 is the schematic diagram at the bottom of getting stuck described in the utility model embodiment；
Fig. 3 a-3d are that the utility model uses result judgement schematic diagram.
Description of reference numerals：
1- gets stuck lid；2- forms；3- whole blood loading slots；4- overflows sample hole；5- sample holes；6- dilution wells；7- gets stuck
Bottom；8- reagent strip screens；9- water conservancy diversion tracks；The excessive sample grooves of 10-；11- guide rings.
Embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the utility model can
To be mutually combined.
In description of the present utility model, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " on ", " under ",
The orientation or position relationship of the instruction such as "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " are
Based on orientation shown in the drawings or position relationship, it is for only for ease of description the utility model and simplifies description, rather than instruction
Or imply that signified device or element must have specific orientation, with specific azimuth configuration and operation, therefore be not understood that
For to limitation of the present utility model.In addition, term " first ", " second " etc. are only used for describing purpose, and it is not intended that instruction
Or imply relative importance or the implicit quantity for indicating indicated technical characteristic.Thus, " first ", " second " etc. are defined
Feature can express or implicitly include one or more this feature.In description of the present utility model, unless separately
It is described, " multiple " are meant that two or more.
, it is necessary to which explanation, unless otherwise clearly defined and limited, term " are pacified in description of the present utility model
Dress ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integratedly
Connection；Can be mechanical connection or electrical connection；Can be joined directly together, can also be indirectly connected by intermediary,
It can be the connection of two element internals.For the ordinary skill in the art, on being understood by concrete condition
State concrete meaning of the term in the utility model.
As shown in Figure 1-2, a kind of whole blood is quantitatively loaded the kit of detection cardiac muscle three, including the lid 1 that gets stuck, bottom 7 of getting stuck,
The described lid 1 that gets stuck is located at the top at described bottom 7 of getting stuck；
Whole blood loading slot 3 is provided with the described lid 1 that gets stuck, the bottom of described whole blood loading slot 3 is provided with sample holes 5,
The side wall of described whole blood loading slot 3 is provided with some excessive sample holes 4, and the side of described whole blood loading slot 3 is provided with dilution
Well 6；
The bottom at described bottom 7 of getting stuck is provided with reagent strip screens 8, be provided with described bottom 7 of getting stuck water conservancy diversion track 9,
Overflow sample groove 10, guide ring 11, and described excessive sample groove 10 is fixed in the side wall at described bottom 7 of getting stuck, described water conservancy diversion track 9
One end connects with excessive sample groove 10, and the other end is fixed on described guide ring 11, and described guide ring 11 is located at described sample introduction
The lower section in hole 5.
Described water conservancy diversion track 9 is obliquely installed, and the upper end of described water conservancy diversion track 9 is connected with described guide ring 11, under
End is connected with described excessive sample groove 10.
The external diameter of the bottom of described whole blood loading slot 3 is more than or equal to the external diameter of described guide ring 11.
Form 2 is provided with the described lid 1 that gets stuck, described form 2 is located at the side of described whole blood loading slot 3.
Described form 2 is located at the top of described reagent strip screens 8.
Liquid is bled into whole blood loading slot 3 when instilling 1, is had 10 microlitres of whole blood samples and is flowed to by sample holes 5 on reagent strip
Used for detection, remaining has more part, and by overflowing, sample hole 4 enters excessive sample groove 10 through guide ring 11, water conservancy diversion guide rail 9, is so protecting
Demonstrate,prove it is easy to operate on the premise of, ensure that sample-adding precision, avoid unnecessary whole blood enter detection reagent bar so as to bring interference.
Describe the utility model in detail below with reference to the accompanying drawings and in conjunction with the embodiments.
In example below, the experimental method of unreceipted actual conditions, generally according to normal condition, or according to manufacturer
Proposed condition.
The preparation of the collaurum of embodiment 1
The heating of 0.02% chlorauric acid solution is boiled, is rapidly added 2% trisodium citrate 2mL；Solution colour is by light blue
Discoloration is dark blue, continuously adds and claret occurs, continues to boil 10min；There is transparent claret, stop heating, continue to stir
Mix to room temperature, obtain colloidal gold solution of the particle diameter in 20nm or so.Electronic Speculum microscopy, it is ensured that the gold grain of preparation makes its size as far as possible
Otherwise consistent and uniform, particle diameter are remake in 20nm or so.
The preparation of colloidal gold pad of the embodiment 2 containing labelled antibody
1.5mL test tubes are taken, add 1mL colloidal gold solutions；Appropriate borate buffer solution is added thereto, and pH is adjusted to 7.6；
10 μ g/mL cTnI labelled antibodies are added, reach minimum protein concentration, are mixed, in quick oscillation 30min on shaker；Add
Enter 10 μ L 10%BSA, mix, vibrate 20min；The 8000rpm in centrifuge, 20min is centrifuged, gently absorbs supernatant；Use 1mL
The loose collaurum precipitation of wash buffer solution resuspensions；Preparing OD (optical density) value at 540nm with fix buffer is
1.2-1.4 colloid gold immune compound；
By above-mentioned colloid gold immune compound and fix buffer according to volume ratio 1:4 ratio mixing, with 2.5 μ l/mm
Amount on glass fibre membrane point sample, form colloidal gold pad, and dry under appropriate conditions, generally 37 DEG C dryings 12 are small
When.
The coating of the nitrocellulose NC films of embodiment 3
Using spray film instrument, 1.0mg/ml cTnI labelled antibodies are uniformly drawn in nitrocellulose NC films with 0.001ml/cm
Upper formation T lines；2.0mg/ml sheep anti mouses polyclonal antibody is uniformly drawn with 0.001ml/cm and forms C on nitrocellulose NC films
Line, and between two lines at intervals of 0.4cm；Drying room is then placed in dry under suitable temperature and humidity.
The assembling of the Test paper of embodiment 4
In the suitable hothouse of condition, the nitre of the coating completion for colloidal gold pad and the step 3 acquisition that step 2 is obtained
Acid cellulose NC films and sample pad and blotting paper are cut into suitable size, are bonded, and control colloidal gold pad is away between T lines
At intervals of 0.4-0.6cm, the interval can be coated with the scribing position, adjustment cut size and bonding portion of NC films by controlling
Size realizes that colloidal gold pad and the bonding portion of NC films are not less than the 1/7 of colloidal gold pad, and colloidal gold pad is bonding with sample pad
Part is the 1/7-1/2 of colloidal gold pad, and the bonding portion of blotting paper and NC films is not less than the 1/10 of blotting paper.It is above-mentioned to be mutually bonded
Each several part can in advance or bonding after be fixed on a loading plate, the loading plate can be plastic plate.Will after the completion of fixation
It is cut to suitable size.The Test paper can with so that be fitted into during foregoing invention mentions.
The Cleaning Principle of embodiment 5 and method
The kit that described whole blood is quantitatively loaded detection cardiac muscle three make use of double antibody in immunochromatographic assays to press from both sides
The principle of heart colour developing, when containing certain density cTnI in whole blood to be measured, after being added into whole blood loading slot 3, wherein 10 is micro-
Rise sample to be entered in sample pad by sample holes 4, unnecessary whole blood sample enters the sample groove 10 that overflows, then in dilution well 6
Whole blood dilution is instilled, whole blood dissolves mouse source cTnI monoclonal antibodies in colloidal gold pad with the liquid in dilution, and
CTnI and the colloid gold label mouse source cTnI monoclonal antibodies are specifically bound, and it is compound to form Ag-Ab-collaurum
Thing, and continue to move to detection line by capillarity.When sample reaches detection line, above-mentioned Ag-Ab-colloidal gold composite
With the cTnI antibody of pairing that is coated in detection line further combined with forming antibody-antigen-antibody-collaurum double antibody folder
Heart compound, and assemble colour developing in detection line.With capillarity further along, when reaching nature controlling line, due to colloid
CTnI monoclonal antibodies are mouses in gold pad, therefore no matter whether contain cTnI in test plasma, are fixed in Quality Control
The sheep anti mouse secondary antibody of line is captured and developed the color, and this colour developing ensure that whole reaction system is correct.
It during detection, blood sample 1 can drip between fetching, tested sample is instilled in whole blood well, is then added in dilution
Whole blood dilution is instilled in sample hole, T lines (lower section lines), the colour developing situation of C lines (top lines) are observed after 20min.C lines show
Color, T line outlets, show that cTnI contents are higher than 500ng/L in sample (see accompanying drawing 3a)；C lines develop the color, and T lines do not develop the color, and show sample
In cTnI levels under 500ng/L (see accompanying drawing 3b)；C lines do not develop the color (see accompanying drawing 3c-3d), show the failure of an experiment, need weight
New test.
Preferred embodiment of the present utility model is the foregoing is only, it is all at this not to limit the utility model
Within the spirit and principle of utility model, any modification, equivalent substitution and improvements made etc., the utility model should be included in
Protection domain within.
Claims (5)
1. a kind of whole blood is quantitatively loaded the kit of detection cardiac muscle three, it is characterised in that：Including lid (1), the bottom of getting stuck of getting stuck
(7), the described lid (1) that gets stuck is located at the top at described bottom of getting stuck (7)；
Whole blood loading slot (3) is provided with the described lid (1) that gets stuck, the bottom of described whole blood loading slot (3) is provided with sample holes
(5), the side wall of described whole blood loading slot (3) is provided with some excessive sample holes (4), the side of described whole blood loading slot (3)
Provided with dilution well (6)；
The bottom at described bottom of getting stuck (7) is provided with reagent strip screens (8), and water conservancy diversion track is provided with described bottom of getting stuck (7)
(9), overflow sample groove (10), guide ring (11), and described excessive sample groove (10) is fixed in the side wall at described bottom of getting stuck (7), described
One end of water conservancy diversion track (9) connect with excessive sample groove (10), the other end is fixed on described guide ring (11), described water conservancy diversion
Ring (11) is located at the lower section of described sample holes (5).
2. whole blood according to claim 1 is quantitatively loaded the kit of detection cardiac muscle three, it is characterised in that：Described leads
Trajectory road (9) is obliquely installed, and the upper end of described water conservancy diversion track (9) is connected with described guide ring (11), lower end with it is described
The sample groove (10) that overflows is connected.
3. whole blood according to claim 1 is quantitatively loaded the kit of detection cardiac muscle three, it is characterised in that：Described is complete
The external diameter of the bottom of blood loading slot (3) is more than or equal to the external diameter of described guide ring (11).
4. whole blood according to claim 1 is quantitatively loaded the kit of detection cardiac muscle three, it is characterised in that：Described card
Form (2) is provided with cap (1), described form (2) is located at the side of described whole blood loading slot (3).
5. whole blood according to claim 4 is quantitatively loaded the kit of detection cardiac muscle three, it is characterised in that：Described regards
Window (2) is located at the top of described reagent strip screens (8).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720566988.7U CN206740772U (en) | 2017-05-19 | 2017-05-19 | A kind of whole blood is quantitatively loaded the kit of detection cardiac muscle three |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720566988.7U CN206740772U (en) | 2017-05-19 | 2017-05-19 | A kind of whole blood is quantitatively loaded the kit of detection cardiac muscle three |
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| Publication Number | Publication Date |
|---|---|
| CN206740772U true CN206740772U (en) | 2017-12-12 |
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| CN201720566988.7U Active CN206740772U (en) | 2017-05-19 | 2017-05-19 | A kind of whole blood is quantitatively loaded the kit of detection cardiac muscle three |
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