CN206038692U - Modified colloidal gold detect reagent board - Google Patents
Modified colloidal gold detect reagent board Download PDFInfo
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- CN206038692U CN206038692U CN201621084738.1U CN201621084738U CN206038692U CN 206038692 U CN206038692 U CN 206038692U CN 201621084738 U CN201621084738 U CN 201621084738U CN 206038692 U CN206038692 U CN 206038692U
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Abstract
The utility model relates to a modified colloidal gold detect reagent board, including reagent strips and reagent board, the reagent board divide into apron and bottom plate, reagent strips includes sample pad, marking pad, the pad that absorbs water, detect membrane and liner, it has detection line and matter accuse line, its characterized in that to detect epimembranal peridium: be provided with sample groove, application of sample hole on the apron and detect the window, the apron inboard still be provided with down and keep off muscle and last fender muscle, lower fender muscle be located the sample groove and detect the window between, it is located the top of detecting the window to keep off the muscle on, set gradually down bayonet socket, bed hedgehopping arch and gone up the bayonet socket from supreme down on the medial surface of bottom plate. The structural design of reagent board is simple reasonable, and reagent strips has obtained fine fixedly in the reagent board, keeps off the muscle with the bed hedgehopping arch is mutually supported down, make the sample median antigen can and marking pad on antibody fully react to reach better detection effect.
Description
Technical field
This utility model is related to a kind of improved gold-immunochromatographyreagent reagent for assay plate, main anti-by the various antigens of colloid gold label
Body detected to various diseases, belongs to vitro diagnostic techniques field.
Background technology
Colloidal gold immunochromatographimethod technology is that twentieth century eight, the nineties develops on the basis of enzyme-linked immune analytic method
New tachysynthesises detection technique.The principle of colloidal gold immunochromatographimethod technology be with nitrocellulose filter as solid phase carrier, with
Used as trace labelling thing, and antibodies, under the percolation of microporous membrane or capillarity, using antigen-antibody for gold colloidal
The distinctive color of high degree of specificity and gold colloidal of reaction carries out spike to gold labeling antibody and antigen or two anti-combinations, shows meat
The visible red stripes of eye or speckle, so that realize the qualitative or quantitative analysis to determinand.
The technical operation is simple and quick, result easily judges, safety non-pollution many advantages, such as make its countries in the world should
With quite varied, become the tendency method of external quick diagnosis field development.At present, colloidal gold chromatographic detection cartoon often will inspection
Survey card to be placed in box body, after sample-adding, the flowing velocity of sample need to be controlled to control the response time, so as to reach preferably detection
Effect.
Utility model content
The purpose of this utility model is to provide a kind of structure design more reasonable, Detection results preferably improved gold colloidal
Detectable plate.
The technical scheme in the invention for solving the above technical problem is：The improved gold-immunochromatographyreagent reagent for assay plate includes
Reagent strip and agent plate, described reagent strip are placed in agent plate, and described agent plate is divided into cover plate and base plate, described examination
Agent bar includes sample pad, label pad, adsorptive pads, detection film and liner, is coated with detection line and nature controlling line on described detection film,
It is characterized in that：Sample slot, well and detection form is provided with described cover plate, is additionally provided with down on the inside of described cover plate
Block reinforcement and upper block reinforcement, described lower block reinforcement are located between sample slot and detection form, and described upper block reinforcement is located at detection form
Top；It is disposed with bayonet, padded raised and upper latch notch on the medial surface of the base plate from bottom to up, the reagent strip
Bottom is placed under bayonet, and the top of the reagent strip is placed in upper latch notch.The inner side width of the under bayonet and upper latch notch
Degree makes reagent strip just snap in and be fixed equal to the width of reagent strip；Described lower block reinforcement and upper block reinforcement can also play solid
Determine the effect of reagent strip.
It is stained with sample pad, label pad, detection film and suction on the upper surface of liner described in the utility model from bottom to up successively
Water cushion；The lower end of the sample pad is concordant with the lower end of liner, and the top of the sample pad is overlapped on the bottom of label pad
And have the lap of 3 ± 1mm；The top of the label pad is overlapped on the bottom of detection film and has the overlapping portion of 2 ± 1mm
Point；The bottom of the adsorptive pads is overlapped on the top of detection film and has the lap of 3 ± 1mm, the adsorptive pads it is upper
End is concordant with the upper end of liner；Described nature controlling line is in the top of detection line.
The preferably described padded raised position for being located at correspondence markings pad on the inside of base plate of this utility model.The padded projection
The region residing for the sample pad and label pad of reagent strip is made to form a low-angle slope, the upper end of label pad is pushed up for slope, can
Slow down the flow velocity that sample flows through label pad, the antigen in sample is more fully reacted with the antibody in label pad, reach more
Good Detection results.
The preferably described lower block reinforcement of this utility model has two, and one is pressed on the nearly upper end of sample pad, another pressure
On the nearly upper end of label pad.Described lower block reinforcement is used for pushing down the nearly upper and lower end of label pad, so as to control the flow velocity of sample,
The antigen in sample is fully reacted with the antibody in label pad, reach more preferable Detection results.In practical application, it is described under
Block reinforcement can be the spot-like projections of raised, corrugated projection of strip etc., or ordered arrangement.
Upper block reinforcement described in the utility model has two, and one is pressed on the nearly upper end of adsorptive pads, and another is pressed in suction
On the nearly lower end of water cushion.Upper block reinforcement is mainly used in pushing down adsorptive pads, whole reagent strip is preferably fixed.Practical application
In, described upper block reinforcement can be the spot-like projections of raised, corrugated projection of strip etc., or ordered arrangement.
Bail is provided with the inside of cover plate described in the utility model, is provided with hole clipping, described card on the inside of the base plate
Hole is blind hole；Described bail is snapped in hole clipping, and bail and hole clipping cooperate.The size phase interworking of the bail and hole clipping
Close, bail can be just snapped in hole clipping, and the quantity and position of bail and hole clipping is corresponded.
Bail described in the utility model and hole clipping respectively have six.Six described bails or hole clipping are arranged at agent plate
Four angles and the both sides at middle part；The bail is snapped in hole clipping one by one, covers described cover plate on base plate and fixed.
Material of the material of the preferably described detection film of this utility model for nitrocellulose filter, the sample pad and label pad
For glass fibre, the material of the adsorptive pads is absorbent filter.
The preferably described well of this utility model is circular hole, and its a diameter of 3 ± 1mm；Described sample slot is inside
The round table-like structure of side depression.Described well is designed to small sircle hole can also be played a part of to slow down sample flow rate.
Buffer system, protected protein and wetting agent has been anticipated in sample pad described in the utility model, can make sample
It is fully absorbed and antigenic component is not destroyed；It is coated with the first antibody of colloid gold label in described label pad in advance,
Can combine with the corresponding antigens in sample；Described detection line for detection film on coated second antibody, also can with sample in
Corresponding antigens combine.After antigen in sample is combined with corresponding first and second antibody, detection line will show
The color of gold colloidal is positive, as in sample without antigen to be detected if do not develop the color and be negative, be exactly more than conventional
The Cleaning Principle of double-antibody method.Described nature controlling line be detect film on coated anti-colloid gold label antibody, can with it is unnecessary
The antibody of colloid gold label combines and develops the color, if nature controlling line does not develop the color, the antibody of colloid gold label has failed, detection knot
It is really insincere.Generally, the antibody of colloid gold label is Mus IgG, and the antibody of anti-colloid gold label is sheep anti-mouse igg.
This utility model has advantages below and effect：The structure design advantages of simple of agent plate, reagent strip is in agent plate
In obtained good fixation, lower block reinforcement and padded projection cooperate, and enable the antibody in antigen in sample and label pad
Fully react, so as to reach more preferable Detection results.
Description of the drawings
Fig. 1 is overall structure diagram of the present utility model；
Fig. 2 is the structural representation side view of this utility model reagent strip；
Fig. 3 is the structural representation top view of this utility model reagent strip；
Fig. 4 is the inside structure schematic diagram of this utility model agent plate cover plate；
Fig. 5 is the inside structure schematic diagram of this utility model agent plate base plate.
In figure：Reagent strip 1, sample pad 11, label pad 12, adsorptive pads 13, detection film 14, liner 15, detection line 16, Quality Control
Line 17,1 width W of reagent strip, 11 length L1 of sample pad, 12 length L2 of label pad, 13 length L3 of adsorptive pads, detection 14 length of film
L4,17 space D of detection line 16 and nature controlling line；Agent plate 2, cover plate 21, base plate 22, well 211, sample slot 212, detection form
213rd, lower block reinforcement 214, upper block reinforcement 215, bail 216, under bayonet 221, padded raised 222, upper latch notch 223, hole clipping 224, two
Lower 214 spacing d of block reinforcement.
Specific embodiment
The utility model is described in further detail below in conjunction with the accompanying drawings and by embodiment.
Embodiment one, Procalcitonin.（PCT）Gold-immunochromatographyreagent reagent for assay plate.
Referring to Fig. 1-5, the present embodiment includes：Reagent strip 1, sample pad 11, label pad 12, adsorptive pads 13, detection film 14, lining
Pad 15, detection line 16, nature controlling line 17, agent plate 2, cover plate 21, base plate 22, well 211, sample slot 212, detection form 213,
Two lower block reinforcements 214, two, 215, six bails 216 of upper block reinforcement, under bayonet 221, padded raised 222, upper latch notch 223, six
Hole clipping 224.
Referring to Fig. 1-3, in the present embodiment, reagent strip 1 includes sample pad 11, label pad 12, adsorptive pads 13, detection film 14
With liner 15, on detection film 14, detection line 16 and nature controlling line 17 is coated with；It is stained with successively on the upper surface of liner 15 from bottom to up
Sample pad 11, label pad 12, detection film 14 and adsorptive pads 13；The lower end of sample pad 11 is concordant with the lower end of liner 15, sample pad
11 top is overlapped on the bottom of label pad 12 and has the lap of 3mm；The top of label pad 12 is overlapped on detection film
On 14 bottom and there is the lap of 2mm；The bottom of adsorptive pads 13 is overlapped on the top of detection film and has the weight of 3mm
Folded part, the upper end of adsorptive pads 13 are concordant with the upper end of liner 15；Nature controlling line 17 is in the top of detection line 16, detection line 16 and matter
17 space D of control line is 5mm；1 width W of reagent strip is 4mm, and 11 length L1 of sample pad is 17mm, and 12 length L2 of label pad is 9mm,
13 length L3 of adsorptive pads is 17mm, and 14 length L4 of detection film is 25mm.
Referring to Fig. 1,4 and 5, in the present embodiment, 2 points of agent plate is cover plate 21 and base plate 22；Sample is provided with cover plate 21
This groove 212, well 211 and detection form 213, manhole of the well 211 for diameter 3mm, from terms of outside, sample slot
212 is the groove of the round table-like structure to inner side depression；Two lower block reinforcements 214 and two upper block reinforcements are additionally provided with the inside of cover plate 21
215, two lower block reinforcements 214 are located between sample slot 212 and detection form 213, and two upper block reinforcements 215 are located at detection form 213
Top, two 214 spacing d of lower block reinforcement are 6mm, and it is raised that two lower block reinforcements 214 and two upper block reinforcements 215 are strip；Bottom
It is disposed with bayonet 221, padded raised 222 and upper latch notch 223 on the medial surface of plate 22 from bottom to up, under bayonet 221 is
Gate side's bayonet socket, padded raised 222 is that gate side is raised, and upper latch notch 223 is made up of two parallel vertical strip projections.
In the present embodiment, the bottom of reagent strip 1 is placed under bayonet 221, and the top of reagent strip 1 is placed on upper latch notch
In 223, the inboard width of under bayonet 221 and upper latch notch 223 is equal to the width of reagent strip 1, i.e. under bayonet 221 and upper latch notch 223
Inboard width be 4mm, reagent strip 1 is just snapped in and is fixed；Padded raised 222 marks for being placed exactly in reagent strip 1
Below note pad 12, will be label pad 12 padded, make sample pad 11 and the region residing for label pad 12 formed one it is low-angle tiltedly
Slope, the upper end of label pad 12 are slope top, can slow down the flow velocity that sample flows through label pad 12, make the antigen in sample and label pad 12
On antibody more fully can react.Six bails 216 are arranged at four angles and the both sides at middle part of 21 medial surface of cover plate, six
Hole clipping 224 is arranged at four angles and the both sides at middle part of base plate medial surface, and six bails 216 are snapped in corresponding hole clipping 224 one by one,
Cover cover plate 21 on base plate 22 and fixed.Two lower block reinforcements 214 of cover plate 21 are press respectively against nearly upper end and the mark of sample pad 11
On the nearly upper end of note pad 12, two upper block reinforcements 215 of cover plate 21 are press respectively against on the nearly upper end and nearly lower end of adsorptive pads 13；
Two lower block reinforcements 214 and padded raised 222 synergy, slow down the flow velocity that sample flows through label pad 12, make the antigen in sample
More fully can react with the antibody in label pad 12, so as to reach more preferable Detection results.
In the present embodiment, the material of detection film 14 for the material of nitrocellulose filter, sample pad 11 and label pad 12 is
Glass fibre, the material of adsorptive pads 13 is absorbent filter；It is coated with the anti-calcitonin of Mus of colloid gold label in label pad 12 in advance
Former monoclonal antibody, detection line 16 are coated with the anti-Procalcitonin monoclonal antibody of another kind of Mus, are coated with sheep anti mouse on nature controlling line
IgG。
The course of work of the present utility model is as follows：
The agent plate for assembling is positioned in clean water flat surface, is dripped 3 in 211 upper vertical of well（120-
150 ul）Sample is instilled in well 211, and sample can be slightly stopped in sample slot 212, slowly penetrate sample pad 11, upwards
Flowing, through label pad 12, detection film 14, is finally absorbed by adsorptive pads 13.After sample-adding, 20-30 minute internal references colorimetric is sticked into
Row result interpretation, if containing Procalcitonin. in sample, detection line 16 will show the color of gold colloidal, without drop calcium such as in sample
Plain principle detection line 16 does not develop the color, by the shade of comparison and detection line 16 from reference to the different colour bands on colorimetric card determining
Procalcitonin. concentration range.It is null result if nature controlling line 17 does not develop the color.
The part being not described in this specification adopts structure known in those skilled in the art and principle,
For prior art.By foregoing description, those skilled in the art can implement.
Furthermore, it is necessary to illustrate, the specific embodiment described in this specification, as long as its part does not clearly state tool
Body specification, then the part can be any specification being adapted with its function；Meanwhile, the title taken by part can not also
Together.The equivalent or simple change done by all constructions described according to this utility model design, feature and principle, is included in practical
In the protection domain of new patent.
Claims (9)
1. a kind of improved gold-immunochromatographyreagent reagent for assay plate, including reagent strip and agent plate, described reagent strip is placed on agent plate
Interior, described agent plate is divided into cover plate and base plate, and described reagent strip includes sample pad, label pad, adsorptive pads, detection film and lining
Pad, is coated with detection line and nature controlling line on described detection film, it is characterised in that：Sample slot is provided with described cover plate, added
Sample hole and detection form, are additionally provided with lower block reinforcement and upper block reinforcement on the inside of described cover plate, described lower block reinforcement be located at sample slot and
Between detection form, described upper block reinforcement is located at the top of detection form；Set successively on the medial surface of the base plate from bottom to up
Under bayonet, padded raised and upper latch notch is equipped with, the bottom of the reagent strip is placed under bayonet, and the top of the reagent strip is put
Put in upper latch notch.
2. improved gold-immunochromatographyreagent reagent for assay plate according to claim 1, it is characterised in that：On the upper surface of the liner
It is stained with sample pad, label pad, detection film and adsorptive pads from bottom to up successively；Hold level with both hands under the lower end of the sample pad and liner
Together, the top of the sample pad is overlapped on the bottom of label pad and has the lap of 3 ± 1mm；The label pad it is upper
Portion is overlapped on the bottom of detection film and has the lap of 2 ± 1mm；The bottom of the adsorptive pads is overlapped on the upper of detection film
On portion and there is the lap of 3 ± 1mm, the upper end of the adsorptive pads is concordant with the upper end of liner；Described nature controlling line is in inspection
The top of survey line.
3. improved gold-immunochromatographyreagent reagent for assay plate according to claim 1, it is characterised in that：Described padded projection is located at
The position of correspondence markings pad on the inside of base plate.
4. improved gold-immunochromatographyreagent reagent for assay plate according to claim 1, it is characterised in that：Described lower block reinforcement has two
Bar, one is pressed on the nearly upper end of sample pad, and another is pressed on the nearly upper end of label pad.
5. improved gold-immunochromatographyreagent reagent for assay plate according to claim 1, it is characterised in that：Described upper block reinforcement has two
Bar, one is pressed on the nearly upper end of adsorptive pads, and another is pressed on the nearly lower end of adsorptive pads.
6. improved gold-immunochromatographyreagent reagent for assay plate according to claim 1, it is characterised in that：The inner side of the cover plate is arranged
There is bail, on the inside of the base plate, be provided with hole clipping, described hole clipping is blind hole；Described bail is snapped in hole clipping, and bail
Cooperate with hole clipping.
7. improved gold-immunochromatographyreagent reagent for assay plate according to claim 6, it is characterised in that：Described bail and hole clipping are each
There are six.
8. improved gold-immunochromatographyreagent reagent for assay plate according to claim 1, it is characterised in that：It is described detection film material be
The material of nitrocellulose filter, the sample pad and label pad is glass fibre, and the material of the adsorptive pads is absorbent filter.
9. improved gold-immunochromatographyreagent reagent for assay plate according to claim 1, it is characterised in that：Described well is circle
Hole, and its a diameter of 3 ± 1mm；Described sample slot is the round table-like structure to inner side depression.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621084738.1U CN206038692U (en) | 2016-09-28 | 2016-09-28 | Modified colloidal gold detect reagent board |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621084738.1U CN206038692U (en) | 2016-09-28 | 2016-09-28 | Modified colloidal gold detect reagent board |
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| Publication Number | Publication Date |
|---|---|
| CN206038692U true CN206038692U (en) | 2017-03-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621084738.1U Active CN206038692U (en) | 2016-09-28 | 2016-09-28 | Modified colloidal gold detect reagent board |
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| CN (1) | CN206038692U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107589268A (en) * | 2017-11-01 | 2018-01-16 | 杭州微瑞科技有限公司 | CDV antibody Quantitative detection card and application method |
| CN107589269A (en) * | 2017-11-01 | 2018-01-16 | 中国农业科学院兰州兽医研究所 | Detection card for A type antibodies against foot-and-mouth disease virus in Quantitative detection serum |
-
2016
- 2016-09-28 CN CN201621084738.1U patent/CN206038692U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107589268A (en) * | 2017-11-01 | 2018-01-16 | 杭州微瑞科技有限公司 | CDV antibody Quantitative detection card and application method |
| CN107589269A (en) * | 2017-11-01 | 2018-01-16 | 中国农业科学院兰州兽医研究所 | Detection card for A type antibodies against foot-and-mouth disease virus in Quantitative detection serum |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Modified colloidal gold detect reagent board Effective date of registration: 20200624 Granted publication date: 20170322 Pledgee: Hangzhou High-tech Financing Guarantee Co.,Ltd. Pledgor: HANGZHOU WATSON BIOTECH Inc. Registration number: Y2020330000396 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220314 Granted publication date: 20170322 Pledgee: Hangzhou High-tech Financing Guarantee Co.,Ltd. Pledgor: HANGZHOU WATSON BIOTECH Inc. Registration number: Y2020330000396 |