CN203132889U - Novel tissue liquid nitrogen clamp favorable to biological tissue preservation - Google Patents
Novel tissue liquid nitrogen clamp favorable to biological tissue preservation Download PDFInfo
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- CN203132889U CN203132889U CN 201220635988 CN201220635988U CN203132889U CN 203132889 U CN203132889 U CN 203132889U CN 201220635988 CN201220635988 CN 201220635988 CN 201220635988 U CN201220635988 U CN 201220635988U CN 203132889 U CN203132889 U CN 203132889U
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- liquid nitrogen
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- pincers
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Abstract
The utility model provides a novel tissue liquid nitrogen clamp favorable to biological tissue preservation. Particularly, in a preparation method for rapidly and effectively freezing a tissue sample, the problem that the quick-freeze effect is poor due to irregularity in the process of rapidly freezing tissue can be solved, the rapidly frozen tissue can be segmented into tissue blocks with experiment required sizes, the subsequent experimental material drawing is convenient, and the experimental efficiency and conclusion accuracy are improved. The liquid nitrogen clamp mainly consists of a pair of bent hemostatic forceps and metal sheets. The clamp is suitable for various tissue extraction in basic medical experiment research, and particularly, the protection on degradable proteins in the tissue is improved.
Description
Technical field
The utility model relates to a kind of experiment apparatus for preparing animal tissue's sample, especially can be when tissue extraction prepares in the preclinical medicine experimental study protection of commute protein degradation.
Background technology
At present, the main path of preclinical medicine experimental study all is by making up the animal model anthropomorphic dummy in the pathological state of body, extracting the Mechanism Study that respective organization is correlated with subsequently; Therefore guarantee that sample is fresh and prevent that protein degradation is most important in the tissue.
Tissue extraction method majority in the past is that organizing of extraction is placed on earlier on ice, also or directly immerses liquid nitrogen, is transferred to-80 ℃ of refrigerators afterwards and preserves.Different owing to the tissue samples size shape in this process, the same piece each several part chilling rate of organizing is inconsistent, not freezing regional cellular metabolism does not simultaneously stop fully, cause that uncontrollable variable effect histone kinase activity takes place for protein level and decorating state in the cell, the experimental design factor is complicated, finally influences follow-up related mechanism research.In addition, the sample that adopts tissue extraction preparation method in the past to preserve, need thaw earlier during use, freezing preservation again after the required big small sample of clip again, so multigelation has greatly destroyed protein content and decorating state in the tissue samples, also make experimental study lack preciseness, experimental data is affected greatly, and inevitably cause the waste of animal used as test and experimental resources.
Summary of the invention
In order to overcome the defective that existing tissue extraction mode occurs in preclinical medicine experimental study different phase, the utility model is by laboratory elbow hemostatic forceps commonly used is transformed, be processed into a kind of novel liquid nitrogen pincers of organizing that biological tissue preserves that are beneficial to, these liquid nitrogen pincers can become a kind of method of sample preparation of effective freezing tissue fast, can not only solve when extracting before the quick-frozen because of the differ problem of the quick-frozen weak effect that causes of rule, and can take the quick-frozen tissue easily, with the piece of organizing of the required size of tissue segmentation one-tenth experiment, the convenient use and raising conventional efficient and conclusion accuracy in follow-up test.
A kind of novel liquid nitrogen pincers of organizing that are beneficial to biological tissue's preservation comprise elbow hemostatic forceps and sheet metal two parts composition, it is characterized in that:
A. described liquid nitrogen pincers adopt operating theater instruments elbow hemostatic forceps as the liquid nitrogen pliers grip;
B. be starting material with the sheet metal, become thickness 2mm ± 1mm through cutting polishing, the circle of diameter 1.5cm ± 1cm, as with the specific purpose tool of organizing direct surface in contact.
This sheet metal be thickness should control more than 1.5mm and sheet thicknesses even, organize each several part stressed basic identical when guaranteeing to use, extract organize stressed can be even.
This elbow hemostatic forceps front end is that firm welding is connected with the sheet metal connected mode, and tie point does not influence the smooth smooth to organizing harmless method of sheet metal and tissue contact surface.
This liquid nitrogen pincers core component adopts the good conductor (being common metal species) of heat, and precooling in liquid nitrogen in advance during use treats that liquid nitrogen pincers sheet metal cools off gripping tissue afterwards fully.
It is the elbow hemostatic forceps of 18cm full-depth tooth that these liquid nitrogen pincers should adopt specification, and namely the suitable length handle can be guaranteed also to organize piece folder flakiness in the operating process rapidly easily, and effectively prevents from extracting liquid nitrogen frostbite experiment operator in the organizational process.
Adopt liquid nitrogen clamp after the above-mentioned precooling to get and organize after the quick-frozen moulding, immediately the hemostatic forceps handle is transferred to closed-bite maloccllusion shelves, and the sheet metal that will accompany tissue immerses in the liquid nitrogen further cooling 1-2 minute to guarantee the organizing piece each several part to cool off fully.
Adopt this typing of liquid nitrogen pincers and complete cooled tissue, the required size of experiment that is divided into that can be easy and convenient is organized piece, and sealing is stored in-80 ℃ of refrigerators after the packing; Need only get the piece of organizing of required size during subsequent experimental, avoid remaining tissue because multigelation influences tissue activity.
The beneficial effects of the utility model are:
The utility model has guaranteed content and the decorating state of albumen in the tissue samples; Can greatly improve experimental data, and save the waste of animal used as test and experimental resources.In addition, the utility model adopts the common elbow hemostatic forceps in laboratory as starting material, and is economical and practical.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1 shows the utility model liquid nitrogen pincers full figure.
Wherein 1 is sheet metal, and 2 is the elbow hemostatic forceps
Fig. 2 shows each position structural representation of the utility model.
Embodiment
The utility model is implemented in the following manner:
Extract tissue samples and earlier hemostatic forceps was immersed the thoroughly pre-cold standby of liquid nitrogen in preceding 5 minutes;
Treat that the animal surgery processing finishes, cut the required piece of organizing rapidly, place between the liquid nitrogen pincers two metal sheets, flatten tissue, and with the hemostatic forceps handle as far as possible folder to closed-bite maloccllusion shelves to enlarge tissue and sheet metal contact area, accelerate tissue cooling and attenuation, the sheet metal that will accompany tissue subsequently immerses again that further cooling 1-2 minute (proper extension cooling time when the tissue piece is bigger than normal) is freezing fully to guarantee organizing each several part in the liquid nitrogen;
This moment, the piece of organizing fully freezing and that become thin slice was subjected to external force very easily broken, can cut apart tissue according to the required size of subsequent experimental in the operating process, airtight being stored in-80 ℃ refrigerator;
In subsequent experimental research, get as required and organize piece, and need not sample is melted.
Claims (5)
1. one kind is beneficial to the novel liquid nitrogen pincers of organizing that biological tissue preserves, and comprises elbow hemostatic forceps (2) and sheet metal (1) two parts composition, it is characterized in that:
A. described liquid nitrogen pincers adopt operating theater instruments elbow hemostatic forceps (2) as the liquid nitrogen pliers grip;
B. be starting material with sheet metal (1), become thickness 2mm ± 1mm through cutting polishing, the circle of diameter 1.5cm ± 1cm, as with the specific purpose tool of organizing direct surface in contact.
2. the novel liquid nitrogen of organizing that is beneficial to biological tissue's preservation according to claim 1 clamps, it is characterized in that: this sheet metal (1) be thickness should control more than 1.5mm and sheet thicknesses even, organize each several part stressed basic identical when guaranteeing to use, extract organize stressed can be even.
3. the novel liquid nitrogen of organizing that is beneficial to biological tissue's preservation according to claim 1 clamps, and it is characterized in that: this elbow hemostatic forceps (2) front end is that firm welding is connected with sheet metal (1) connected mode.
4. the novel liquid nitrogen pincers of organizing that are beneficial to biological tissue's preservation according to claim 1 is characterized in that: the good conductor of this liquid nitrogen pincers core component employing heat.
5. the novel liquid nitrogen of organizing that is beneficial to biological tissue's preservation according to claim 4 clamps, and it is characterized in that: it is the elbow hemostatic forceps of 18cm full-depth tooth that these liquid nitrogen pincers should adopt specification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220635988 CN203132889U (en) | 2012-11-27 | 2012-11-27 | Novel tissue liquid nitrogen clamp favorable to biological tissue preservation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220635988 CN203132889U (en) | 2012-11-27 | 2012-11-27 | Novel tissue liquid nitrogen clamp favorable to biological tissue preservation |
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| Publication Number | Publication Date |
|---|---|
| CN203132889U true CN203132889U (en) | 2013-08-14 |
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| CN 201220635988 Expired - Fee Related CN203132889U (en) | 2012-11-27 | 2012-11-27 | Novel tissue liquid nitrogen clamp favorable to biological tissue preservation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105973635A (en) * | 2016-06-29 | 2016-09-28 | 廖丹丹 | Mobile device used in preparation process of biological tissue sample slices |
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2012
- 2012-11-27 CN CN 201220635988 patent/CN203132889U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105973635A (en) * | 2016-06-29 | 2016-09-28 | 廖丹丹 | Mobile device used in preparation process of biological tissue sample slices |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130814 Termination date: 20141127 |
|
| EXPY | Termination of patent right or utility model |