CN1963514A - Test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis - Google Patents
Test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis Download PDFInfo
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- CN1963514A CN1963514A CN 200510086852 CN200510086852A CN1963514A CN 1963514 A CN1963514 A CN 1963514A CN 200510086852 CN200510086852 CN 200510086852 CN 200510086852 A CN200510086852 A CN 200510086852A CN 1963514 A CN1963514 A CN 1963514A
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- antibody
- capsular
- test strips
- anthrax
- bacillus anthracis
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Abstract
This invention provides one glue gold test bar to test anthrax bacillus antibody, which covers anthrax bacillus antigen and IgG on NC film and combines glue gold label capsular antigen single clone antibody and applies film analysis antibody clamper method. This invention is used to test label anthrax bacillus antigen as assistant function for anthrax affection.
Description
Technical field
The invention belongs to field of biological detection, be specifically related to a kind of test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis and application thereof.
Background technology
Anthrax (anthrax) is a kind of infecting both domestic animals and human acute infectious disease that is caused by Bacillus anthracis (Bacillus anthrax).Herdsman, peasant, fur and the worker that butchers are easily infected.
Since the eighties, at anthrax laboratory diagnosis technical elements significant progress has been arranged, antibody detection method commonly used has: ELISA method, enzyme mark-SPA method, PCR method, fluorescent immune method etc.
But these detection techniques and actual demand still have many gaps, mainly show: need special instrument and equipment, and require the professional and technical personnel to detect in the laboratory of specialty, and complicated operation, length consuming time is unwell to on-the-spot instant the detection.
Summary of the invention
(1) technical matters that will solve
The purpose of this invention is to provide a kind of test strips that detects the Bacillus anthracis capsular antigen easily and efficiently, the inventor is by wrapping simultaneously on nitrocellulose membrane (NC film) by anti-capsular antigen antibody (monoclonal antibody or polyclonal antibody) and anti-mouse IgG two bands, and the anti-capsular antigen monoclonal antibody of application association colloid gold mark, realized having finished the present invention with the purpose of test strips fast detecting Bacillus anthracis capsular antigen.Overcome prior art complicated operation, length consuming time, needed specific apparatus and professional operating personnel's deficiency.
(2) technical scheme
The invention provides a kind of test strip, it is characterized in that, it comprises:
(1) bag is by the nitrocellulose membrane of anthrax capsular antibody (monoclonal antibody or polyclonal antibody) and anti-mouse IgG two bands of Quality Control;
(2) contain the glass fibre membrane of colloid gold label anthrax pod membrane monoclonal antibody.
Preferred test strips of the present invention, it also comprises:
(1) reaction holder;
(2) adsorptive pads;
(3) golden labeling antibody diaphragm.
Wherein react the preferred PVC plate of holder; The preferred filter paper for oil of adsorptive pads; Gold labeling antibody diaphragm has absorbent function simultaneously, preferably uses polyester film, glass fibre or filter paper fibre.
The invention also discloses the application of described test strips in detecting the Bacillus anthracis capsular antigen.
Detection sample of the present invention can be: the secretion of skin lesion, phlegm, vomitus, excreta, blood, cerebrospinal fluid.
Technical scheme of the present invention is: the Bacillus anthracis capsular antibody (monoclonal antibody or how anti-) and the anti-mouse IgG difference solid phase (NC film) on nitrocellulose membrane that adopt purifying, the pod membrane monoclonal antibody of association colloid gold mark is used rete and is analysed Bacillus anthracis in the principle detection sample of double antibody sandwich method.
(3) beneficial effect
The invention has the beneficial effects as follows: detect the Bacillus anthracis capsular antigen with test strips, the positive evidence that can provide anthrax to infect plays the auxiliary detection effect to the diagnosis of anthrax.This method is easy, quick, accurate, does not need special instruments and equipment, does not need professional training, and testing result is clear easily to be distinguished, simple to operate, is easy to promote, and is fit to the instant detection and the large-scale epidemiology survey of grass-roots units such as hospital.
Description of drawings
Fig. 1: the front schematic view of A test strips of the present invention;
The side schematic view of B test strips of the present invention;
1: adsorptive pads;
2: (T: bag is by Bacillus anthracis capsular antibody band for nitrocellulose membrane; C: bag is by the Quality Control band of anti-mouse IgG);
3: the glass fibre membrane that contains the capsular antigen monoclonal antibody of colloid gold label;
4: golden labeling antibody diaphragm;
5: the reaction holder.
Fig. 2: testing result synoptic diagram;
Be followed successively by from left to right: two line colour developings of T, C are positive; Line colour developing of C is negative; Two lines of T, C do not develop the color for invalid.
Embodiment
The reaction holder is 6.5cm * 0.4cm PCV plate; Adsorptive pads is the filter paper for oil of 2cm * 0.4cm; The nitrocellulose membrane of 2cm * 0.4cm wraps successively by anti-mouse IgG, Bacillus anthracis capsular antibody; The glass fibre membrane that contains the capsular antigen monoclonal antibody of 0.4cm * 0.4cm colloid gold label; Gold labeling antibody diaphragm is the polyester film of 2.5cm * 0.4cm; Promptly formed test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis.
The reaction holder is 6.5cm * 0.4cm PCV plate; Adsorptive pads is the filter paper for oil of 2cm * 0.4cm; The nitrocellulose membrane of 2cm * 0.4cm wraps successively by anti-mouse IgG, Bacillus anthracis capsular antibody; The glass fibre membrane that contains the capsular antigen monoclonal antibody of 0.4cm * 0.4cm colloid gold label; Gold labeling antibody diaphragm is the glass fibre of 2.5cm * 0.4cm; Promptly formed test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis.
The reaction holder is 6.5cm * 0.4cm PCV plate; Adsorptive pads is the filter paper for oil of 2cm * 0.4cm; The nitrocellulose membrane of 2cm * 0.4cm wraps successively by anti-mouse IgG, Bacillus anthracis capsular antibody; The glass fibre membrane that contains the capsular antigen monoclonal antibody of 0.4cm * 0.4cm colloid gold label; Gold labeling antibody diaphragm is the filter paper fibre of 2.5cm * 0.4cm; Promptly formed test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis.
Embodiment 4 detection methods (referring to Fig. 2)
Relevant regulations according to the detection of anthrax diagnostic criteria national standard (GB17015-1997) laboratory, leave and take the secretion of skin lesion, put into the bottle that cell pyrolysis liquid is housed, embodiment 1 test strips " 4 " is located to insert bottle interior (can take out test strips after observation finishes fruit), liquid in the sample picks up up, 10-15 minute sentence read result:
As containing the Bacillus anthracis capsular antigen in the sample, then with test strips on the capsular antigen monoclonal antibody of colloid gold label form corresponding compound, up be coated on nitrocellulose membrane on capsular antibody combine, assemble to form red lines, promptly locate to form red stripes at " T ".
No matter whether contain corresponding antigen, the monoclonal antibody of colloid gold label continues upwards to creep and the anti-mouse IgG that is coated on the film forms the red precipitate line, promptly locates to form red Quality Control band at " C ".This line does not occur, and illustrates that test strips lost efficacy.
Experimental example: clinical detection result
The secretion of 70 routine patient suspecteds' skin lesion is compared with present application ELISA method (T1) after testing, 16 routine T, the colour developing of C two bands, and concrete outcome sees the following form.
ELISA method (T1) and test strips method (T2) contrast experiment statistical form
| Positive | Negative | |
| ?T1 | ?17 | ?53 |
| ?T2 | ?16 | ?54 |
| Sensitivity 94% | Specificity 100% |
Claims (7)
1, a kind of test strip is characterized in that, it comprises:
(1) bag is by the nitrocellulose membrane of anthrax capsular antibody and anti-mouse IgG two bands of Quality Control;
(2) contain the glass fibre membrane of colloid gold label anthrax pod membrane monoclonal antibody.
2, according to the described test strips of claim 1, the anthrax capsular antibody that is coated on the nitrocellulose membrane is monoclonal antibody or polyclonal antibody.
3, according to the described test strips of claim 1, it also comprises:
(1) reaction holder;
(2) adsorptive pads;
(3) golden labeling antibody diaphragm.
4,, wherein react holder and select the PVC plate for use according to the described test strips of claim 3.
5, according to the described test strips of claim 3, wherein adsorptive pads is selected filter paper for oil for use.
6, according to the described test strips of claim 3, wherein golden labeling antibody diaphragm is selected polyester film, glass fibre or filter paper fibre for use.
7, the application of the arbitrary described test strips of claim 1-6 in detecting the Bacillus anthracis capsular antigen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510086852 CN1963514A (en) | 2005-11-10 | 2005-11-10 | Test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510086852 CN1963514A (en) | 2005-11-10 | 2005-11-10 | Test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1963514A true CN1963514A (en) | 2007-05-16 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510086852 Pending CN1963514A (en) | 2005-11-10 | 2005-11-10 | Test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1963514A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102353776A (en) * | 2011-06-07 | 2012-02-15 | 中国科学院武汉病毒研究所 | Magnetic immunochromatographic test strip for quantitative test of anthrax spores and preparation method thereof |
| CN102353787A (en) * | 2011-09-27 | 2012-02-15 | 山东莱博生物科技有限公司 | Colloidal gold test paper strip for semiquantitatively detecting concentration of GFAP (Glial Fibrillary Acidic Protein) in human serum |
-
2005
- 2005-11-10 CN CN 200510086852 patent/CN1963514A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102353776A (en) * | 2011-06-07 | 2012-02-15 | 中国科学院武汉病毒研究所 | Magnetic immunochromatographic test strip for quantitative test of anthrax spores and preparation method thereof |
| CN102353787A (en) * | 2011-09-27 | 2012-02-15 | 山东莱博生物科技有限公司 | Colloidal gold test paper strip for semiquantitatively detecting concentration of GFAP (Glial Fibrillary Acidic Protein) in human serum |
| CN102353787B (en) * | 2011-09-27 | 2013-03-06 | 山东莱博生物科技有限公司 | Colloidal gold test paper strip for semiquantitatively detecting concentration of GFAP (Glial Fibrillary Acidic Protein) in human serum |
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Open date: 20070516 |