CN1748569A - Method for preparing calcium hyauronate - Google Patents
Method for preparing calcium hyauronate Download PDFInfo
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- CN1748569A CN1748569A CNA2005101173009A CN200510117300A CN1748569A CN 1748569 A CN1748569 A CN 1748569A CN A2005101173009 A CNA2005101173009 A CN A2005101173009A CN 200510117300 A CN200510117300 A CN 200510117300A CN 1748569 A CN1748569 A CN 1748569A
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- calcium
- hyauronate
- alcoholic solution
- salt
- solution
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Abstract
The present invention provides the preparation process of calcium hyauronate. Inside alcohol solution, sodium hyauronate and calcium salt are made to produce direct ion exchange to calcium hyauronate. The present invention has simple technological process, less apparatus needed and low cost.
Description
Technical field
The invention belongs to field of health care food, relate to a kind of preparation method of calcium hyauronate.
Background technology
Calcium hyauronate is hyaluronic calcium salt, the polysaccharide that hyaluronic acid (hyaluronic acid) is made up of the disaccharide recurring unit of glucuronic acid and aminoglucose, be present in many connective tissues such as skin, vitreum, umbilical cord, cartilage, knuckle synovia, and play therein preserve moisture, physiological actions such as nutrition, reparation and pre-antisitic defect.Calcium hyauronate can be used as capsule or solution, and hyaluronic acid has good cosmetic result, and calcium hyauronate is again good calcium tonic, therefore as health food, not only has cosmetic result but also have the good health care effect.
Traditional preparation technology is a spent ion exchange resin, and with the Sodium Hyaluronate dissolving, upper prop changes into calcium hyauronate by ion-exchange with Sodium Hyaluronate, again by organic solvent deposit, and dehydration, vacuum drying.The method yield is low, and the production cycle is long, complex procedures, and use equipment is many, and the present invention only finishes at dehydrating step, and the yield height, use equipment is few, and technology is simple and easy to do.
Summary of the invention
The invention provides a kind of preparation method of calcium hyauronate, be with the Sodium Hyaluronate pressed powder in the alcoholic solution of calcium salt by the method for ion-exchange, directly change into calcium hyauronate.
Organic solvent of the present invention comprises methyl alcohol and ethanol.
Calcium salt of the present invention, for calcium chloride, calcium sulfate, calcium acetate, calcium nitrate etc. can be dissolved in the inorganic or organic zinc salt of little molecule of alcohol-aqueous solution, wide material sources are easy to get.
Its concentration of alcohol-aqueous solution of the present invention is 40%～100% (volume fraction)
This invention is solid powdery product, calcium content 3%～12%.
The preparation technology of calcium hyauronate is in this invention: A. is soaked into Sodium Hyaluronate in the alcoholic solution that contains calcium salt, stirs to be beneficial to the speeding-up ion exchange, filters, and removes alcoholic solution.B. repeat A step 2～10 time, obtain the wet solid of calcium hyauronate.C. soak the wet solid of washing calcium hyauronate with the alcoholic solution that does not contain calcium salt.D. the dry calcium hyauronate that gets.
The specific embodiment of the invention:
Following examples are for illustrating in greater detail the present invention, are not restriction the present invention.
Embodiment 1
With Sodium Hyaluronate 180 grams, stir, add 2 liters of ethanolic solutions (concentration of alcohol 92%) that contain 1.5% calcium sulfate, open stirring, after 12 hours, stop to stir, the alcohol supernatant inclines, repeat displacement step 8 times, wash 5 times with 95% ethanol afterwards, to alcoholic strength 95%, centrifugal, remove ethanol, put the vacuum drying chamber drying, to moisture 7%.
Embodiment 2
With Sodium Hyaluronate 230 grams, stir, add 2.5 liters of methyl alcohol (methanol concentration 90%) that contain 3% calcium chloride, open stirring, after 12 hours, stop to stir, the methyl alcohol supernatant inclines, adding 2 liters of methyl alcohol that contain calcium chloride again repeats to replace 8 times, with analyzing pure methanol dehydration 5 times, centrifugal afterwards, remove methyl alcohol, put the vacuum drying chamber drying, to moisture 7%.
Embodiment 3
With Sodium Hyaluronate 200 grams, stir, add 2 liters of ethanol (concentration of alcohol 90%) that contain 3% calcium acetate, open stirring, after 12 hours, stop to stir the ethanol that inclines supernatant, adding 2 liters of ethanol that contain calcium acetate again repeats to replace 8 times, use 90% ethanol dehydration 5 times afterwards, centrifugal, remove ethanol, put the vacuum drying chamber drying, to moisture 7%.
Claims (6)
- The preparation method of 1 one kinds of calcium hyauronates.
- Calcium hyauronate described in 2 claims 1 is called glass acid calcium again.
- Calcium hyauronate described in 3 claims 1] the preparation method, it is characterized in that with the Sodium Hyaluronate pressed powder in the alcoholic solution of calcium salt by the method for ion-exchange, directly change into calcium hyauronate.
- Calcium salt described in 4 claims 3, for calcium chloride, calcium sulfate, calcium acetate, calcium nitrate etc. can be dissolved in the inorganic or organic calcium salt of little molecule of alcoholic solution, the concentration of calcium salt in alcoholic solution is 1%～15%.
- Alcoholic solution is the aqueous solution of methyl alcohol or ethanol described in 5 claims 3 or 4, and its solution concentration is 40%～100% (volume fraction).
- 6 this invent and be solid powdery product, calcium content 3%～12%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101173009A CN1326883C (en) | 2005-11-04 | 2005-11-04 | Method for preparing calcium hyauronate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101173009A CN1326883C (en) | 2005-11-04 | 2005-11-04 | Method for preparing calcium hyauronate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1748569A true CN1748569A (en) | 2006-03-22 |
| CN1326883C CN1326883C (en) | 2007-07-18 |
Family
ID=36604370
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101173009A Active CN1326883C (en) | 2005-11-04 | 2005-11-04 | Method for preparing calcium hyauronate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1326883C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831004A (en) * | 2010-03-24 | 2010-09-15 | 章云 | Hyaluronic acid strontium salt compounds, preparation method thereof and application in medicine |
| CN102834417A (en) * | 2010-04-14 | 2012-12-19 | 丘比株式会社 | Process for producing hyaluronic acid metal salt, process for producing cosmetic containing hyaluronic acid metal salt, and zinc hyaluronate and process for producing same |
| WO2023125789A1 (en) * | 2021-12-31 | 2023-07-06 | 华熙生物科技股份有限公司 | Calcium hyaluronate, and preparation method therefor and use thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0622560B2 (en) * | 1989-04-11 | 1994-03-30 | 株式会社紀文食品 | Method for producing hyaluronate sheet |
| DK1526859T3 (en) * | 2002-08-07 | 2013-02-04 | Medidom Lab | Process for preparing a sterile high molecular weight hyaluronic acid preparation |
| CN1537868A (en) * | 2003-04-18 | 2004-10-20 | 上海建华精细生物制品有限公司 | Preparation method of sodium hyaluronic acid |
| CN1563108A (en) * | 2004-04-13 | 2005-01-12 | 阮春学 | Method for preparing transparent calcium hyaIuronate in low molecular weight |
-
2005
- 2005-11-04 CN CNB2005101173009A patent/CN1326883C/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831004A (en) * | 2010-03-24 | 2010-09-15 | 章云 | Hyaluronic acid strontium salt compounds, preparation method thereof and application in medicine |
| CN102834417A (en) * | 2010-04-14 | 2012-12-19 | 丘比株式会社 | Process for producing hyaluronic acid metal salt, process for producing cosmetic containing hyaluronic acid metal salt, and zinc hyaluronate and process for producing same |
| CN102834417B (en) * | 2010-04-14 | 2015-11-25 | 丘比株式会社 | The manufacture method of hyaluronic acid metal salt, the manufacture method comprising the makeup of hyaluronic acid metal salt and zinc hyaluronate and manufacture method thereof |
| WO2023125789A1 (en) * | 2021-12-31 | 2023-07-06 | 华熙生物科技股份有限公司 | Calcium hyaluronate, and preparation method therefor and use thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1326883C (en) | 2007-07-18 |
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Owner name: SHANDONG FREDA BIOPHARM CO., LTD. Free format text: FORMER NAME: FURUIDA BIOCHEMICAL CO., LTD., SHANDONG |
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Address after: 250014 No. 264 mountain road, Lixia District, Shandong, Ji'nan Patentee after: Shandong Freda Biopharm Co., Ltd. Address before: 250014 No. 264 mountain road, Lixia District, Shandong, Ji'nan Patentee before: Furuida Biological Chemical Co., Ltd., Shandong |
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