CN1615726A - Green fodder additive specially for high quality meat chicken - Google Patents
Green fodder additive specially for high quality meat chicken Download PDFInfo
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- CN1615726A CN1615726A CNA2003101085969A CN200310108596A CN1615726A CN 1615726 A CN1615726 A CN 1615726A CN A2003101085969 A CNA2003101085969 A CN A2003101085969A CN 200310108596 A CN200310108596 A CN 200310108596A CN 1615726 A CN1615726 A CN 1615726A
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Abstract
The present invention relates to green additive specially for high quality meat chicken fodder, relates to fodder additive prepared with microbes, and aims at replacing the additive for antibiotic in fodder resulting in lowered chicken immunity and antibiotic residue. The additive includes probiotics and photosynthetic bacteria in the concentration of 1E6-1E10/ml, and the probiotics are one or several of bifidobacterium, faecal streptococci, Bcillus subtilis and licheniformis bacillus. The present invention is mainly used in fodder industry.
Description
Technical field
The present invention relates to feed addictive, especially a kind of feed addictive that utilizes the microorganism preparation.
Background technology
For satisfying people's demand growing to meat product, the aquaculture of recent two decades China progressively to industrialization, grouping of the world economy producer to development, thereby promoted the rapid formation of feed industry.China's feed annual production is 5.2 hundreds of millions tons (2000) at present, accounts for world's total amount 1/10th, becomes another production of fodder big country after the U.S..Green feed is meant and can not uses in the feed or do not contain harmful antibiotic, the core of feed is a feed addictive, additives function can be divided into nutrition, health care, improves feed quality, increase aspects such as appetite, wherein human body is had a direct impact and what endanger is antibiotic in the health care additive.At the medium-term and long-term antibiotic that uses of animal feed, suppressed beneficial microbe, reduced the immunity of animal body, certain bacterium of animal body is developed immunity to drugs; And at its product---residual in meat, egg, the milk, brought hidden danger to food security and health.Country clearly stipulates antibiotic in the additive: 1. antibiotic that bans use of such as chloramphenicol; 2. the antibiotic such as the terramycin of limiting the quantity of and using.Green feed is as a kind of new industry, it is the main trend of new century modern agricultural development, exploitation and popularization substitute antibiotic green feed additive, be that the green ecological aquaculture is subjected to more and more that people pay attention to and pays close attention to, the insider thinks that microorganism formulation is a feeding antibiotic substitute preferably from now on.Probiotic is a kind of work, can effectively promote body to regulate the microorganism formulation of microecological balance in the enteron aisle, the key technology of development probiotic is the good production flora of screening, makes active bacteria formulation through artificial the cultivation, is used for animal then and brings into play its intrinsic physiological action.The quality of bacterial classification is directly connected to effect and products thereof quality.Drugs approved by FDA is as existing 43 kinds of the microorganism directly fed, and wherein lactic acid bacteria is 28 kinds, 5 kinds of bacillus, 4 kinds of bacteroids, 2 kinds of Aspergillus, 2 kinds in saccharomycete etc.China Ministry of Agriculture announces that the feed level microbe additive that allows to use has 12 kinds, is divided into lactic acid bacteria class, gemma mushroom and budding fungus.Probiotic is existing many application in poultry feed: 1.Broman etc. add probiotic in the fryer grain ration, broiler weightening finish 5%; 2. Yan's oecoeclades falcata etc. (1996) adds bacillus subtilis in the meat chick fodder, and Bacillus acidi lactici quantity increases in the chicken enteron aisle, and salmonella quantity reduces, and feed conversion rate improves.
Summary of the invention
The purpose of this invention is to provide a kind of meat high-quality chicken special-purpose non-toxic feed addictive, use antibiotic in order to solve in the existing broiler fodder, bacterium in the chicken body is developed immunity to drugs and reduced the immunity of chicken body, and the food security that antibiotic residue causes in the chicken, problem such as be detrimental to health.
The object of the present invention is achieved like this: high quality meat chicken special-purpose non-toxic feed addictive of the present invention, and use acid probio early stage, and the later stage is used alkaline photosynthetic bacteria; The concentration of probio and photosynthetic bacteria is every milliliter 10
8～10
10Bacterial strain.
Wherein probio is one or more of Bifidobacterium Bifidum, streptococcus fecalis, bacillus subtilis and bacillus licheniformis; Photosynthetic bacteria is defined as two to three bacterial strains at present, and relevant title is still unnamed, and usefulness is the Mixed Microbes of two bacterial strains wherein at present.
Wherein above-mentioned probio and photosynthetic bacteria provide bacterial classification by the academy of agricultural sciences, Shanghai City, and are preserved by this unit.Preserving number is: Bifidobacterium Bifidum SNC99-03, streptococcus fecalis SNC99-05, bacillus subtilis SNC99-06, bacillus licheniformis SNC99-07, photosynthetic bacteria numbering SNC98-09.
The acid probio that the present invention selects for use is by the separation and purification of intact animal enteron aisle, observes through colony shape, and biochemical identification, electron microscopic observation and animal toxicity test etc. are the normal beneficial bacteriums of animal and bird intestines, it has the obvious suppression effect to Escherichia coli.In earlier stage use probio at growth of meat chicken, compare test with using antibiotic, photosynthetic bacteria, result of the test proof probiotic effect is best, and the fryer death rate is zero.
The alkaline photosynthetic bacteria that the present invention selects for use is the photosynthetic bacteria that is widely used in livestock and poultry and aquatic feeds both at home and abroad, it plays an important role in natural carbon, ammonia, sulfur cycle, its thalline is nontoxic, nutritious, protein content contains multivitamin up to 60～65%, as solatene, VB family, folic acid, biotin etc., having anti-disease substance, promote growth factor and ubiquinone, also is a kind of natural colouring matter.Use photosynthetic bacteria in the growth of meat chicken later stage, compare test with using antibiotic, probio, aspects such as result of the test proof feedstuff-meat ratio, pigmentation, death rate all are better than the antibiotic and the probio that contrast.
The manufacture method of acid probio of the present invention is as follows:
(1) be that glucose 1.5%～0.5%, animal blood 7%～2%, peptone 3%～1%, surplus are that the weight proportion of water is made nutrient solution with the percentage by weight;
(2) above-mentioned probio Mixed Microbes is inoculated in this nutrient solution;
(3) cultivation temperature is 30～34 ℃, incubation time 20～28 hours.
The manufacture method of alkaline photosynthetic bacteria of the present invention is as follows:
(1) be that glucose 1.5%～0.5%, animal blood 8%～3%, surplus are that the weight proportion of water is made nutrient solution with the percentage by weight;
(2) the photosynthetic bacterial classification of above-mentioned alkalescence is inoculated in this nutrient solution;
(3) the control cultivation temperature is 30～35 ℃, cultivates illumination from liquid level 15～25 luxs, incubation time 10～16 days.
Compared with prior art, the present invention has following advantage:
1. the feed of antibiotic-free additive raises fryer and does not contain antibiotic residue, has ensured food security and health;
The full phase survival rate of fryer with improve with weightening finish, the production cost of fryer is descended;
3. the trunk dressing percentage of fryer improves, and the meat color and luster is attractive in appearance, and das Beinfleisch is tender, bright, and brisket does not have tart flavour, features good taste.
The specific embodiment
With embodiment the present invention is further described below, but the present invention is not limited to these embodiment.Any change that those skilled in the art are done under the enlightenment of disclosure of the present invention also all drops in the scope of claim protection of the present invention.Concentration among the embodiment is weight percent concentration.
Embodiment 1
(1) be that the weight proportion of water is made nutrient solution with glucose 1.5%, animal blood 7%, peptone 3%, surplus;
(2) be that main mixing probio is inoculated in this nutrient solution with Bifidobacterium Bifidum;
(3) cultivation temperature is 30 ℃, incubation time 20 hours.
Embodiment 2
(1) be that the weight proportion of water is made nutrient solution with glucose 1%, animal blood 5%, peptone 2%, surplus;
(2) be that main mixing probio is inoculated in this nutrient solution with streptococcus fecalis;
(3) the control cultivation temperature is 32 ℃, incubation time 24 hours.
Embodiment 3
(1) be that the weight proportion of water is made nutrient solution with glucose 0.5%, animal blood 2%, peptone 1%, surplus;
(2) be that main mixing probio is inoculated in this nutrient solution with bacillus licheniformis;
(3) the control cultivation temperature is 34 ℃, incubation time 28 hours.
Embodiment 4
(1) be that the weight proportion of water is made nutrient solution with glucose 1.5%, animal blood 8%, surplus;
(2) the photosynthetic bacterial classification of alkalescence is inoculated in this nutrient solution;
(3) cultivation temperature is 30 ℃, cultivates illumination from liquid level 15 luxs, incubation time 10 days.
Embodiment 5
(1) be that the weight proportion of water is made nutrient solution with glucose 1%, animal blood 5%, surplus;
(2) the photosynthetic bacterial classification of alkalescence is inoculated in this nutrient solution;
(3) cultivation temperature is 32 ℃, cultivates illumination from liquid level 20 luxs, incubation time 14 days.
Embodiment 6
(1) be that the weight proportion of water is made nutrient solution with glucose 0.5%, animal blood 3%, surplus;
(2) the photosynthetic bacterial classification bacterium of alkalescence is inoculated in this nutrient solution;
(3) cultivation temperature is 35 ℃, cultivates illumination from liquid level 25 luxs, incubation time 16 days.
Embodiment 7
Be sample test in probio of the present invention, the supporting additive of photosynthetic bacteria, (use photosynthetic bacteria with probio, later stage early stage).Feeding experiment is divided two batches, and 1000 of the every batch of chickens are raised three months (91 days), and test group is established two groups of drinking-water, spices, and the test material adds probio, the supporting additive of photosynthetic bacteria but added with antibiotic not.Control group is 185,482 and 483 3 sections system material of market, Shanghai fryer commercial feed.Result of the test is as follows:
1. survive and increase weight: full phase survival rate test group is 97%, improves 2% than control group 95%, and the weight increment test group increases by 5% than control group.
2. trunk and meat: slaughter determining, dressing percentage is higher than control group for two groups, is respectively 2.9% and 3.3%, and complete clean thorax dressing percentage is tested one group and is lower than 3.3%, two group of contrast and is higher than contrast 1.5%.It is attractive in appearance that meat is tested one group of color and luster, and das Beinfleisch is obvious every, delicate flavour, and brisket does not have tart flavour, and it is all better than one group and contrast to test two groups of chicken tastes.
3. residual quantity detects: get five of chickens in the test group, butcher and get chest, das Beinfleisch 300 grams, liver is whole, the positive chicken of control group is established three groups, be respectively terramycin, aureomycin and chloramphenicol, every group of 5 chickens, feed is fed the various antibiotic of 100PPM, butcher simultaneously with the test group chicken after five days, send residual quantity to detect.Test group muscle, internal organ all do not detect terramycin, aureomycin and residual chloromycetin, and positive chicken detects terramycin, aureomycin and residual chloromycetin respectively.
Claims (5)
1. a high quality meat chicken special-purpose non-toxic feed addictive is characterized in that: comprising acid probio or alkaline photosynthetic bacteria; Concentration is every milliliter 10
8～10
10Bacterial strain.
2. high quality meat chicken special-purpose non-toxic feed addictive as claimed in claim 1 is characterized in that: wherein probio is one or more in Bifidobacterium Bifidum, streptococcus fecalis, bacillus subtilis and the bacillus licheniformis.
3. the manufacture method of high quality meat chicken special-purpose non-toxic feed addictive as claimed in claim 1 is characterized in that: its mid-early stage is acid probio, and the later stage is alkaline photosynthetic bacteria.
4. the manufacture method of high quality meat chicken special-purpose non-toxic feed addictive according to claim 1, wherein additive is a probio, it is characterized in that comprising the steps:
(1) be that glucose 1.5%～0.5%, animal blood 7%～2%, peptone 3%～1%, surplus are that the weight proportion of water is made nutrient solution with the percentage by weight;
(2) above-mentioned probio Mixed Microbes is inoculated in this nutrient solution;
(3) the control cultivation temperature is 30～34 ℃, incubation time 20～28 hours.
5. the manufacture method of high quality meat chicken special-purpose non-toxic feed addictive as claimed in claim 1, wherein additive is a photosynthetic bacteria, it is characterized in that comprising the steps:
(1) be that glucose 1.5%～0.5%, animal blood 8%～3%, surplus are that the weight proportion of water is made nutrient solution with the percentage by weight;
(2) the photosynthetic bacterial classification of above-mentioned alkalescence is inoculated in this nutrient solution;
(3) cultivation temperature is 30～35 ℃, cultivates illumination from liquid level 15～25 luxs, incubation time 10～16 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101085969A CN1615726A (en) | 2003-11-13 | 2003-11-13 | Green fodder additive specially for high quality meat chicken |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101085969A CN1615726A (en) | 2003-11-13 | 2003-11-13 | Green fodder additive specially for high quality meat chicken |
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| Publication Number | Publication Date |
|---|---|
| CN1615726A true CN1615726A (en) | 2005-05-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2003101085969A Pending CN1615726A (en) | 2003-11-13 | 2003-11-13 | Green fodder additive specially for high quality meat chicken |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210413A (en) * | 2011-06-27 | 2011-10-12 | 陕西大秦汉集团有限公司 | Dorking microorganism viable bacteria fermentation concentrated feed and production method thereof |
| CN102379365A (en) * | 2011-09-29 | 2012-03-21 | 滨州市正元畜牧发展有限公司 | Non-resistance cultivation method with joint application of padding strain, feed strain and disinfectant bacterium |
| CN101606581B (en) * | 2008-06-16 | 2012-05-30 | 灵宝上洋科技有限责任公司 | Green feed additive and preparation method thereof |
-
2003
- 2003-11-13 CN CNA2003101085969A patent/CN1615726A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101606581B (en) * | 2008-06-16 | 2012-05-30 | 灵宝上洋科技有限责任公司 | Green feed additive and preparation method thereof |
| CN102210413A (en) * | 2011-06-27 | 2011-10-12 | 陕西大秦汉集团有限公司 | Dorking microorganism viable bacteria fermentation concentrated feed and production method thereof |
| CN102210413B (en) * | 2011-06-27 | 2012-08-22 | 陕西大秦汉集团有限公司 | Dorking microorganism viable bacteria fermentation concentrated feed and production method thereof |
| CN102379365A (en) * | 2011-09-29 | 2012-03-21 | 滨州市正元畜牧发展有限公司 | Non-resistance cultivation method with joint application of padding strain, feed strain and disinfectant bacterium |
| CN102379365B (en) * | 2011-09-29 | 2013-07-10 | 滨州市正元畜牧发展有限公司 | Non-resistance cultivation method with joint application of padding strain, feed strain and disinfectant bacterium |
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