CN1196712C - Human like collagen and production method thereof - Google Patents
Human like collagen and production method thereof Download PDFInfo
- Publication number
- CN1196712C CN1196712C CN01106757.8A CN01106757A CN1196712C CN 1196712 C CN1196712 C CN 1196712C CN 01106757 A CN01106757 A CN 01106757A CN 1196712 C CN1196712 C CN 1196712C
- Authority
- CN
- China
- Prior art keywords
- gap
- gpp
- gpa
- gsa
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010035532 Collagen Proteins 0.000 title claims abstract description 77
- 102000008186 Collagen Human genes 0.000 title claims abstract description 76
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 17
- 229920001436 collagen Polymers 0.000 title claims description 44
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 12
- 238000007670 refining Methods 0.000 claims abstract description 3
- GVVPGTZRZFNKDS-JXMROGBWSA-N geranyl diphosphate Chemical compound CC(C)=CCC\C(C)=C\CO[P@](O)(=O)OP(O)(O)=O GVVPGTZRZFNKDS-JXMROGBWSA-N 0.000 claims description 174
- 108020004414 DNA Proteins 0.000 claims description 22
- 238000013016 damping Methods 0.000 claims description 13
- 239000012530 fluid Substances 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- 238000000746 purification Methods 0.000 claims description 6
- 230000001939 inductive effect Effects 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 239000012634 fragment Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 239000013522 chelant Substances 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 230000000968 intestinal effect Effects 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 230000008521 reorganization Effects 0.000 claims description 3
- 238000011218 seed culture Methods 0.000 claims description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical group C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 2
- 108020004511 Recombinant DNA Proteins 0.000 claims description 2
- 239000003456 ion exchange resin Substances 0.000 claims description 2
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 2
- 229910052751 metal Inorganic materials 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 2
- 239000008351 acetate buffer Substances 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 12
- 102000004169 proteins and genes Human genes 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 7
- 230000008859 change Effects 0.000 abstract description 6
- 238000000576 coating method Methods 0.000 abstract description 3
- 239000011248 coating agent Substances 0.000 abstract description 2
- 238000010353 genetic engineering Methods 0.000 abstract description 2
- 210000000056 organ Anatomy 0.000 abstract description 2
- 238000005215 recombination Methods 0.000 abstract description 2
- 230000006798 recombination Effects 0.000 abstract description 2
- 229910052709 silver Inorganic materials 0.000 abstract description 2
- 239000004332 silver Substances 0.000 abstract description 2
- -1 silver halide Chemical class 0.000 abstract description 2
- 239000011247 coating layer Substances 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 241000588724 Escherichia coli Species 0.000 description 6
- 229920000936 Agarose Polymers 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000003277 amino acid sequence analysis Methods 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000010195 expression analysis Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000003119 immunoblot Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 235000011073 invertase Nutrition 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 210000002826 placenta Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- BZQFBWGGLXLEPQ-UHFFFAOYSA-N O-phosphoryl-L-serine Natural products OC(=O)C(N)COP(O)(O)=O BZQFBWGGLXLEPQ-UHFFFAOYSA-N 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000012084 conversion product Substances 0.000 description 1
- 229950006137 dexfosfoserine Drugs 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 102000034240 fibrous proteins Human genes 0.000 description 1
- 108091005899 fibrous proteins Proteins 0.000 description 1
- 239000007888 film coating Substances 0.000 description 1
- 238000009501 film coating Methods 0.000 description 1
- 229940009493 gel-one Drugs 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000011654 magnesium acetate Substances 0.000 description 1
- 229940069446 magnesium acetate Drugs 0.000 description 1
- 235000011285 magnesium acetate Nutrition 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 1
- 210000005059 placental tissue Anatomy 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 229960004249 sodium acetate Drugs 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (6)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN01106757.8A CN1196712C (en) | 2001-02-21 | 2001-02-21 | Human like collagen and production method thereof |
PCT/CN2002/000424 WO2003106494A1 (en) | 2001-02-21 | 2002-06-14 | A human collagen-like protein and the method of producing it |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN01106757.8A CN1196712C (en) | 2001-02-21 | 2001-02-21 | Human like collagen and production method thereof |
PCT/CN2002/000424 WO2003106494A1 (en) | 2001-02-21 | 2002-06-14 | A human collagen-like protein and the method of producing it |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1371919A CN1371919A (en) | 2002-10-02 |
CN1196712C true CN1196712C (en) | 2005-04-13 |
Family
ID=32231731
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN01106757.8A Expired - Lifetime CN1196712C (en) | 2001-02-21 | 2001-02-21 | Human like collagen and production method thereof |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN1196712C (en) |
WO (1) | WO2003106494A1 (en) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100366302C (en) | 2006-09-14 | 2008-02-06 | 清华大学 | Mineralized polypeptide material in use for repairing bones, and preparation method |
CN101200718B (en) * | 2006-12-11 | 2013-04-17 | 南京理工高新技术发展有限公司 | Human-like collagen gene, tandem gene in same direction and with different repeat numbers, recombinant plasmid having tandem gene and preparation method |
CN102061296A (en) * | 2010-08-20 | 2011-05-18 | 吉林农业大学 | Preparation method of water-soluble human collagen VI polypeptide |
CN102020716B (en) * | 2010-10-29 | 2012-03-07 | 陕西东大生化科技有限责任公司 | Gene recombination human collagen fusion peptide segment, preparation method and application thereof |
CN102351954B (en) * | 2011-10-26 | 2013-12-18 | 陕西巨子生物技术有限公司 | Recombinant collagen |
CN102492032B (en) * | 2011-11-16 | 2014-05-21 | 陕西巨子生物技术有限公司 | Human-like collagen and injectable human-like collagen soft-tissue filling material |
CN102492033A (en) * | 2011-11-16 | 2012-06-13 | 陕西巨子生物技术有限公司 | Human-like collagen and human-like collagen composite sodium hyaluronate hydrogel |
CN105709267B (en) * | 2015-12-04 | 2018-10-12 | 陕西巨子生物技术有限公司 | A kind of human-like collagen cell migration gel |
CN105709266B (en) * | 2015-12-04 | 2018-10-12 | 陕西巨子生物技术有限公司 | A kind of human-like collagen scar reparation Silica hydrogel |
JP2019510517A (en) | 2016-03-29 | 2019-04-18 | ジェルター, インコーポレイテッド | Expression of proteins in Gram-negative bacteria with periplasmic volume to intracytoplasmic volume ratios between 0.5: 1 and 10: 1 |
CN106540314B (en) * | 2016-11-25 | 2019-10-08 | 陕西巨子生物技术有限公司 | A kind of human-like collagen nasal membrane reparation gel |
CN107033238B (en) * | 2017-05-03 | 2020-09-01 | 河南***生物药业有限公司 | Purification method and preparation method of recombinant human III-type collagen |
CN109608551A (en) * | 2018-12-04 | 2019-04-12 | 西北大学 | A kind of HLC-BMP fusion protein and preparation method thereof |
CN111333715B (en) * | 2020-04-23 | 2021-04-13 | 江南大学 | Preparation method of type I collagen fiber |
US11639377B2 (en) | 2020-04-23 | 2023-05-02 | Jiangnan University | Preparation of type I collagen-like fiber and method for regulating and controlling the D-periodic of fiber thereof |
CN111375088B (en) * | 2020-04-29 | 2022-06-14 | 陕西巨子生物技术有限公司 | Double-layer osteochondral tissue repair scaffold and preparation method thereof |
CN111467560B (en) * | 2020-05-27 | 2022-08-05 | 陕西巨子生物技术有限公司 | Medical hemostatic dressing, preparation method and application thereof |
CN112316214B (en) * | 2020-11-03 | 2022-09-30 | 陕西巨子生物技术有限公司 | Injectable hydrogel of recombinant collagen and preparation method thereof |
CN114539389B (en) * | 2022-02-22 | 2023-01-31 | 陕西巨子生物技术有限公司 | Recombinant collagen and application thereof |
CN117229390A (en) * | 2023-09-20 | 2023-12-15 | 山东义才和锐生物技术有限公司 | Preparation method of recombinant III type humanized collagen |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995031473A1 (en) * | 1994-05-11 | 1995-11-23 | Organogenesis Inc. | Collagen from cell cultures |
EP0859838B1 (en) * | 1995-08-31 | 2003-06-04 | The Victoria University Of Manchester | Novel procollagens |
-
2001
- 2001-02-21 CN CN01106757.8A patent/CN1196712C/en not_active Expired - Lifetime
-
2002
- 2002-06-14 WO PCT/CN2002/000424 patent/WO2003106494A1/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
WO2003106494A1 (en) | 2003-12-24 |
CN1371919A (en) | 2002-10-02 |
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Legal Events
Date | Code | Title | Description |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20021002 Assignee: XI'AN GIANT BIOGENE TECHNOLOGY CO., LTD. Assignor: Fan Daidi Contract record no.: 2010610000010 Denomination of invention: Human like collagen and production method thereof Granted publication date: 20050413 License type: Exclusive License Record date: 20100409 |
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TR01 | Transfer of patent right |
Effective date of registration: 20170622 Address after: 710077 Xi'an high tech Zone, Jin Industrial Road, No., No. C Venture Park, No. 20, Patentee after: XI'AN GIANT BIOGENE TECHNOLOGY CO., LTD. Address before: 710075 Department of chemical engineering, Northwestern University, Shaanxi, Xi'an Patentee before: Fan Daidi |
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TR01 | Transfer of patent right | ||
CX01 | Expiry of patent term |
Granted publication date: 20050413 |
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CX01 | Expiry of patent term |