CN117598926A - Skin barrier repair composition containing recombinant collagen and application thereof - Google Patents
Skin barrier repair composition containing recombinant collagen and application thereof Download PDFInfo
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- CN117598926A CN117598926A CN202311625054.2A CN202311625054A CN117598926A CN 117598926 A CN117598926 A CN 117598926A CN 202311625054 A CN202311625054 A CN 202311625054A CN 117598926 A CN117598926 A CN 117598926A
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- skin barrier
- barrier repair
- recombinant collagen
- skin
- emulsion
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- 239000005017 polysaccharide Substances 0.000 description 1
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- 239000008213 purified water Substances 0.000 description 1
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- 230000001105 regulatory effect Effects 0.000 description 1
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- 238000012216 screening Methods 0.000 description 1
- 210000002374 sebum Anatomy 0.000 description 1
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- 230000036620 skin dryness Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4953—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom containing pyrimidine ring derivatives, e.g. minoxidil
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
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- Mycology (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to a skin barrier repair composition containing recombinant collagen and application thereof, wherein the skin barrier repair composition comprises recombinant collagen, conopeptide, schizophyllan and ectoin. The invention creatively combines the four components of the recombinant collagen, the conopeptide, the schizophyllan and the ectoin, which all-directionally improve the problem of skin barrier damage from different dimensions and strengthen the skin barrier, and the four components complement each other to have potential mutual promotion effects, thus having synergistic effect on the effects and being capable of effectively improving the comprehensive problem of skin caused by the skin barrier damage.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and relates to a skin barrier repair composition containing recombinant collagen and application thereof.
Background
Healthy skin can effectively retain moisture, thereby maintaining a good elastic and shiny appearance of the skin. However, the skin is affected by living environment and physiological conditions, and the skin may have the problems of sensitivity, dryness, desquamation, excessive secretion of grease, color spots, loose skin aging, red blood streaks and the like, and the most main reason is that the skin barrier is damaged. The skin barrier is a protective structure which is jointly constructed by the cuticle and the sebum membrane, can buffer physical and chemical stimulation, effectively inhibit bacterial growth, prevent damage of bacteria, viruses and fungi to skin, and can lock moisture and prevent moisture evaporation, thereby realizing the function of maintaining moisture. The skin barrier is damaged, which causes the skin to be eroded by bacteria, viruses and fungi, and also causes the moisture in the skin to evaporate unscrupulously, so that various problems such as susceptibility to sensitization, xerosis cutis, loose skin aging and the like occur on the skin.
At present, the common means for repairing skin barriers is to fully supplement water, but most of water in the traditional cosmetics only exists on the surface layer of the skin, the evaporation rate of the water on the surface layer of the skin is far greater than the absorption rate, the water quantity which can be absorbed by the skin is limited, the skin barriers are slowly repaired, the time consumption is long, and the simple water supplement has no effective improvement effect on skin sensitivity problems and skin relaxation and aging.
CN116172934a discloses a composition with skin barrier repair function and moisturizing function and application thereof, comprising the following components in parts by mass: 0.1-20 parts of nutgrass galingale rhizome extract, 0.3-20 parts of boswellia serrata extract, 0.2-20 parts of myrrh leaf cell extract, 1-30 parts of glycerin and 10-90 parts of deionized water. The composition has obvious effects of relieving skin dryness, recovering skin epidermis barrier and improving skin moisture content. However, the composition has a slow and long time-consuming effect on skin barrier repair, and cannot effectively improve the skin comprehensive problem caused by the damage of the skin barrier.
CN109394595a discloses a mask essence with deep water supplementing and barrier repairing functions, wherein the formula comprises the following components in parts by weight: 10-97%, purified water: 0-90%, allantoin: 0.1-0.2%, polyol: 1-8%, polyglycerol humectant: 0.5-2%, disodium EDTA: 0.02-0.1%, and umarones: 0.3-0.6%,1, 2-hexanediol: 0.3-0.6%, carbomer: 0.1-0.2%, arginine: 0.1-0.2%, sodium hyaluronate: 0.01-0.1%, hydroxyethyl cellulose: 0.01-0.1%, hydroxylated lecithin: 0.1-0.2%; has deep water supplementing effect, and barrier repairing effect. However, the product has a slow and long time-consuming effect on repairing skin barriers, and cannot effectively improve the comprehensive problem of skin caused by damaged skin barriers.
Therefore, it is highly desirable to develop a product that can effectively ameliorate the skin integration problem caused by damaged skin barrier.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a skin barrier repair composition containing recombinant collagen and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a skin barrier repair composition comprising recombinant collagen, conopeptides, schizophyllan and ectofactor.
The conopeptide has the effects of accelerating local blood circulation and local metabolism, stimulating the activity of skin, enhancing the activity of skin cells, delaying skin aging, and obtaining excellent skin care effect due to rich nutrition of the components. The schizophyllan polysaccharide can activate the activities of skin immune cells and other tissue cells, improve the resistance and repair capability of the whole skin tissue, and has good skin moisturizing function. The ectoin has potential in the aspects of anti-aging, ultraviolet resistance, oxidation resistance, moisture preservation, inflammation inhibition and the like, can improve the immune protection capability of skin cells, and has better antiallergic and anti-inflammatory effects; the cell repair capability is improved, so that the skin can effectively resist invasion of microorganisms and allergens, has good protection and repair effects on the skin, and accelerates recovery of skin health. In the aspect of ultraviolet resistance, the ectoin can repair cell DNA damage caused by ultraviolet rays, can effectively reduce the generation of sunburn cells and prevent the cell membrane structure from being damaged. The collagen can timely supplement collagen for skin, strengthen skin barrier, resist environmental injury, and keep skin in a healthy state.
The invention creatively combines the four components of the recombinant collagen, the conopeptide, the schizophyllan and the ectoin, which all-directionally improve the problem of skin barrier damage from different dimensions and strengthen the skin barrier, and the four components complement each other to have potential mutual promotion effects, thus having synergistic effect on the effects and being capable of effectively improving the comprehensive problem of skin caused by the skin barrier damage.
Preferably, the skin barrier repair composition comprises, by mass, 0.01-2 parts of recombinant collagen, 0.1-3 parts of conopeptide, 0.1-0.5 part of schizophyllan and 0.1-1 part of ectoin.
Based on the potential interactions between the above components, when the above four components are combined in a specific mass ratio relationship, they have a better effect in strengthening the skin barrier and improving the skin integration problem caused by the damage of the skin barrier.
The weight portion of the recombinant collagen can be selected from 0.01 portion, 0.03 portion, 0.05 portion, 0.08 portion, 0.1 portion, 0.2 portion, 0.4 portion, 0.5 portion, 0.8 portion, 1 portion, 1.2 portion, 1.4 portion, 1.5 portion, 1.8 portion, 2 portion and the like; the mass parts of the conopeptide can be selected from 0.1 part, 0.3 part, 0.5 part, 0.8 part, 1 part, 1.2 parts, 1.4 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.5 parts, 2.8 parts, 3 parts and the like; the schizophyllan is selected from 0.1 part, 0.15 part, 0.2 part, 0.25 part, 0.3 part, 0.35 part, 0.4 part, 0.45 part, 0.5 part and the like by mass part; the mass parts of the ectoine can be selected from 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like. Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the nucleotide sequence encoding the recombinant collagen is the sequence shown in SEQ ID NO. 1.
Compared with other sources of collagen or recombinant collagen, the recombinant collagen from the specific source has better effect in strengthening skin barrier and improving skin comprehensive problems caused by skin barrier damage.
SEQ ID NO:1:
GGGCCTCAAGGTATTGCTGGACAGCGTGGTGTGGTCGGCCTGCCTGGTCAGAGAGGAGAGAGAGGCTTCCCTGGTCTTCCTGGCCCCTCTGGTGAACCTGGCAAACAAGGTCCCTCTGGAGCAAGTGGTGAACGTGGTCCCCCTGGTCCCATGGGCCCCCCTGGATTGGCTGGACCCCCTGGTGAATCTGGACGTGAGGGGGCTCCTGGTGCCGAAGGTTCCCCTGGACGAGACGGTTCTCCTGGCGCCAAGGGTGACCGTGGTGAGACCGGCCCCGCTGGACCCCCTGGTGCTCCTGGTGCTCCTGGTGCCCCTGGCCCCGTTGGCCCTGCTGGCAAGAGTGGTGATCGTGGTGAGACTGGTCCTGCTGGTCCCGCCGGAGAACGAGGTGGCCCTGGAGGACCTGGCCCTCAGGGTCCTCCTGGAAAGAATGGTGAAACTGGACCTCAGGGACCCCCAGGGCCTACTGGGCCTGGTGGTGACAAAGGAGACACAGGACCCCCTGGTCCACAAGGATTACAAGGCTTGCCTGGTACAGGTGGTCCTCCAGGAGAAAATGGAAAACCTGGGGAACCAGGTCCAAAGGGTGATGCCGGTGCACCTGGAGCTCCAGGAGGCAAGGGTGATGCTGGTGCCCCTGGTGAACGTGGACCTCCTGGATTGGCAGGGGCCCCAGGACTTAGAGGTGGAGCTGGTCCCCCTGGTCCCGAAGGAGGAAAGGGTGCTGCTGGTCCTCCTGGGCCACCTGGTGCTGCTGGTACTCCTGGTCTGCAAGGAATGCCTGGACCTGGTCCTTGCTGTGGTGGTTAA。
In a second aspect, the present invention provides the use of a skin barrier repair composition comprising recombinant collagen according to the first aspect for the preparation of a cosmetic.
Preferably, the skin barrier repair composition comprising recombinant collagen is present in the cosmetic in an amount of 0.1-15% by mass, e.g. 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 10%, 11%, 12%, 13%, 15% etc. Other specific point values within the numerical range can be selected, and will not be described in detail herein.
In a third aspect, the invention provides an emulsion for strengthening skin barrier repair, wherein the emulsion comprises the following preparation raw materials in percentage by mass: the skin barrier repair composition comprising recombinant collagen according to the first aspect comprises 0.1-15%, oil phase 1-20%, emulsifier 1-10% and water 50-90%.
The mass percentage of the skin barrier repair composition may be selected to be 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 10%, 11%, 12%, 13%, 15%, etc.; the mass percentage of the oil phase can be selected to be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 15%, 18%, 20% and the like; the mass percentage of the emulsifier can be selected to be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10% and the like; the water may be selected to be 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% by mass, etc. Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the oil phase comprises any one or a combination of at least two of glycerol caprylate, squalane, olive oil, white pool seed oil, jojoba oil, shea butter, sweet almond oil, grape seed oil, caprylic/capric triglyceride, hydrogenated cocoglyceride, phytosterol isostearate, isononyl isononanoate, arachidyl alcohol, vitamin a, vitamin E or vitamin F. Preferably, a combination of white pool flower seed oil, sweet almond oil, grape seed oil and vitamin E.
Preferably, the emulsifier comprises any one or a combination of at least two of glycerol stearate, cetostearyl alcohol, sodium stearoyl lactylate, glycerol stearate citrate, cholesterol, cetostearyl alcohol oleyl oleate, sorbitan stearate, polyglycerol-10 oleate or polysorbate-60.
Preferably, the preparation raw materials of the emulsion further comprise, in mass percent: 1-15% of plant source conditioning agent, 0.1-2% of pH regulator, 0.01-0.5% of chelating agent, 0.05-0.5% of antioxidant and 0.1-0.5% of aromatic agent or the combination of at least two.
The mass percentage of the plant source conditioning agent can be selected to be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 10%, 11%, 12%, 13%, 15% and the like; the mass percentage of the pH regulator can be selected to be 0.1%, 0.3%, 0.5%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2% and the like; the mass percentage of the chelating agent can be selected to be 0.01%, 0.03%, 0.05%, 0.08%, 0.1%, 0.15%, 0.2%, 0.3%, 0.4%, 0.5% and the like; the antioxidant may be selected from 0.05%, 0.08%, 0.1%, 0.15%, 0.2%, 0.3%, 0.4%, 0.5%, etc.; the mass percentage of the aromatic agent can be selected to be 0.1%, 0.15%, 0.2%, 0.3%, 0.4%, 0.5% and the like; other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the plant source conditioner comprises witch hazel extract and/or erigeron breviscapus extract.
The invention also creatively discovers that when the witch hazel extract and/or the erigeron breviscapus extract, especially the combination of the witch hazel extract and the erigeron breviscapus extract, are further added into the product, the effect of the product in the aspect of barrier repair can be further improved. Wherein Hamamelis mollis extract and erigeron breviscapus extract have synergistic effect in exerting the above effects.
In a fourth aspect, the present invention provides a method for preparing an emulsion for enhancing skin barrier repair according to the third aspect, the method comprising the steps of:
(1) Mixing water and skin barrier repair composition at 25-40deg.C, homogenizing to obtain water phase; mixing the oil phase with emulsifier at 35-50deg.C, and homogenizing to obtain oil phase;
(2) Mixing and homogenizing the water phase, the oil phase and the rest preparation raw materials at 25-40 ℃ to obtain the emulsion for reinforcing skin barrier repair.
The preparation process of the emulsion for reinforcing skin barrier repair is simple and easy to operate, and is very suitable for industrial production.
The specific point value of 25-40deg.C can be selected from 25deg.C, 30deg.C, 35deg.C, 40deg.C, etc.; the specific point value of 35-50deg.C can be 35 deg.C, 40 deg.C, 45 deg.C, 50 deg.C, etc.; other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Compared with the prior art, the invention has the following beneficial effects:
the invention creatively combines the four components of the recombinant collagen, the conopeptide, the schizophyllan and the ectoin, which all-directionally improve the problem of skin barrier damage from different dimensions and strengthen the skin barrier, and the four components complement each other to have potential mutual promotion effects, thus having synergistic effect on the effects and being capable of effectively improving the comprehensive problem of skin caused by the skin barrier damage.
Detailed Description
In order to further describe the technical means adopted by the present invention and the effects thereof, the following describes the technical scheme of the present invention in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
The mass parts of each component in the composition product referred to in the following are calculated according to the actual content of the effective components in the raw materials sold in the market.
The Hamamelis extract referred to in the following is derived from the product of Watershiz Biotechnology Inc., lanzhou; the erigeron breviscapus extract is derived from the product of Waterson Biotechnology Co., ltd; schizophyllan is derived from products of the biotechnology company, volterras, lanzhou. Other raw materials are all common raw materials in the field and are all purchased through a commercial way.
The recombinant collagen referred to in the following was prepared using the product prepared in preparation examples in which pET-28a (+) vector was purchased from Michelin; BL21 (DE 3) chemocompetent cells were commercially available from Merck under the designation CMC0014-4X40 UL.
Preparation example
The preparation example provides recombinant collagen freeze-dried powder, and the preparation method is carried out by referring to the method disclosed in the preparation example 1 in ZL202310123476.3, and specifically comprises the following steps:
(1) Constructing a recombinant plasmid: according to the nucleotide sequence shown in SEQ ID NO. 1, selecting a pET-28a (+) vector suitable for an expression vector escherichia coli host, and sending the pET-28a (+) vector to a sequencing company for synthesis to prepare a recombinant collagen expression vector pET-28a-1.
(2) Recombinant fermentation strain construction: transferring the pET-28a-1 expression vector into competent cells of escherichia coli (BL 21 (DE 3)) by using a chemical conversion method, screening positive clones in plate colonies after conversion by agarose gel electrophoresis, transferring the screened positive clones to 2mL of LB culture medium, culturing for 12 hours at 37 ℃ and 200rpm, diluting the cultured bacterial liquid by 1 time with glycerol with the volume fraction of 50%, packaging into a freezing tube, and freezing at the temperature of minus 80 ℃ to obtain the recombinant collagen fermentation strain.
(3) Fermenting and culturing recombinant fermentation strains: 10 mu L of recombinant collagen fermentation broth is coated on a solid LB plate, cultured for 12 hours in a 37 ℃ incubator, selected and inoculated in 50mL of LB liquid medium containing 50 mu g/mL kanamycin content, placed in a shaking table for culture at 200rpm for 14 hours at 37 ℃, then inoculated in 200mL of LB liquid medium containing 50 mu g/mL kanamycin content at a ratio of 1:100, cultured until the bacterial broth OD=0.6 at 37 ℃, added with IPTG to achieve the final concentration of 1mM, continuously cultured for 4 hours, then centrifuged at 4000rpm for 15 minutes, and the bacterial cells are collected.
(4) Purifying recombinant collagen: the bacterial cells are subjected to ultrasonic disruption and centrifugation, the precipitate is washed by using a TritonX-100 washing solution, the supernatant is removed by centrifugation again, 6M guanidine hydrochloride is added into the precipitate, the pH is regulated to 8, after the precipitate is fully dissolved, the solution is subjected to nickel affinity chromatography resin, 20mM, 50mM, 100mM, 200mM and 500mM concentration gradient imidazole solution is used for eluting in sequence, eluent is concentrated by using a protein concentration column, the concentrated solution is collected and dialyzed overnight (dialysis bag, RC film, 14KD and the flat width is 44mM (Producer F600112)), then the protein concentration is detected by using a BCA kit, and the solution is uniformly mixed with the mannitol aqueous solution according to the proportion of 0.5mL of 5% mannitol aqueous solution by volume concentration per 5mg protein, and then the solution is pre-frozen at-40 ℃ for 10min by using a freeze dryer, and finally freeze-dried at-35 ℃ to obtain the recombinant collagen freeze-dried powder.
Example 1
The embodiment provides a skin barrier repair composition, which comprises the following components in parts by mass: 0.1 part of recombinant collagen, 0.5 part of conopeptide, 0.3 part of schizophyllan and 0.2 part of ectoin. The components are physically and evenly mixed according to the mass ratio.
Example 2
The embodiment provides a skin barrier repair composition, which comprises the following components in parts by mass: 0.05 part of recombinant collagen, 1 part of conopeptide, 0.1 part of schizophyllan and 0.5 part of ectoin. The components are physically and evenly mixed according to the mass ratio.
Example 3
The embodiment provides a skin barrier repair composition, which comprises the following components in parts by mass: 0.1 part of recombinant collagen, 0.2 part of conopeptide, 0.5 part of schizophyllan and 0.2 part of ectoin. The components are physically and evenly mixed according to the mass ratio.
Comparative example 1
This comparative example provides a composition which differs from example 1 only in that it does not contain recombinant collagen, and its reduced mass is apportioned to the mass of conopeptide, schizophyllan and ectofactor, the other components and contents remaining unchanged. The components are physically and evenly mixed according to the mass ratio.
Comparative example 2
This comparative example provides a composition differing from example 1 only in that no conopeptides were included, and their reduced mass was apportioned to the mass of recombinant collagen, schizophyllan and ectofactor, with the other components and amounts remaining unchanged. The components are physically and evenly mixed according to the mass ratio.
Comparative example 3
This comparative example provides a composition which differs from example 1 only in that it does not contain schizophyllan, and its reduced mass is apportioned to the mass of conopeptide, recombinant collagen and ectoin, the other components and contents remaining unchanged. The components are physically and evenly mixed according to the mass ratio.
Comparative example 4
This comparative example provides a composition differing from example 1 only in that it does not contain ectoin, and its reduced mass is apportioned to the mass of conopeptides, schizophyllan and recombinant collagen, with the other components and contents remaining unchanged. The components are physically and evenly mixed according to the mass ratio.
Application example 1
The application example provides an emulsion for strengthening skin barrier repair, which comprises the following components:
the preparation method comprises the following steps:
(1) Mixing water and skin barrier repair composition at 30deg.C, homogenizing to obtain water phase; mixing the oil phase with the emulsifier at 40 ℃ and homogenizing to obtain an oil phase;
(2) And mixing and homogenizing the water phase, the oil phase and the rest preparation raw materials at 40 ℃ to obtain the emulsion for reinforcing skin barrier repair.
Application examples 2 to 3
The present application example provides two emulsions for enhancing skin barrier repair, the formulation of which differs from that of application example 1 only in that the skin barrier repair composition of example 1 is replaced with the skin barrier repair compositions prepared in examples 2 to 3 in equal amounts, and the other components and contents remain unchanged. The preparation method is reference application example 1.
Application example 4
The application example provides an emulsion for strengthening skin barrier repair, the formula of the emulsion is different from that of application example 1 only in that white pond flower seed oil is lacked, the reduced mass of the emulsion is distributed to the mass of sweet almond oil and grape seed oil in proportion, and other components and contents are kept unchanged. The preparation method is reference application example 1.
Application example 5
The application example provides an emulsion for strengthening skin barrier repair, the formula of the emulsion is different from that of application example 1 only in that sweet almond oil is lacked, the reduced mass of the emulsion is distributed to the mass of the white pool flower seed oil and the grape seed oil in proportion, and other components and contents are kept unchanged. The preparation method is reference application example 1.
Application example 6
The application example provides an emulsion for strengthening skin barrier repair, the formula of the emulsion is different from that of application example 1 only in that grape seed oil is lacked, the reduced mass of the emulsion is distributed to the mass of the white pond flower seed oil and the sweet almond oil in proportion, and other components and contents are kept unchanged. The preparation method is reference application example 1.
Comparative application examples 1 to 4
Comparative examples 1-4 provided four emulsions for enhancing skin barrier repair, the formulation of which differed from that of example 1 only in that the skin barrier repair composition of example 1 was replaced with the skin barrier repair composition of comparative examples 1-4 in equal amounts, with the other components and amounts remaining unchanged. The preparation method is reference application example 1.
Comparative application example 5
Comparative application example 5 provides an emulsion whose formulation differs from application example 1 only in the absence of the skin barrier repair composition, whose reduced quality is complemented by water, the other components and the content remaining unchanged.
Application example 7
The application example provides an emulsion for strengthening skin barrier repair, which comprises the following components:
the preparation method comprises the following steps:
(1) Mixing water and skin barrier repair composition at 30deg.C, homogenizing to obtain water phase; mixing the oil phase with the emulsifier at 40 ℃ and homogenizing to obtain an oil phase;
(2) Mixing the aqueous phase and the oil phase with the plant source conditioner and the rest of the preparation raw materials at 40 ℃ for homogenizing to obtain the emulsion for reinforcing skin barrier repair.
Application example 8
The application example provides an emulsion for strengthening skin barrier repair, and the formula of the emulsion is different from that of application example 4 only in that the witch hazel extract is lacking, the mass percentage of the erigeron breviscapus extract is 8%, and other components and contents are kept unchanged.
Application example 9
The application example provides an emulsion for strengthening skin barrier repair, and the formula of the emulsion is different from that of application example 4 only in that the emulsion lacks erigeron breviscapus extract, the mass percentage of the witch hazel extract is 8%, and other components and contents are kept unchanged.
Test example 1
Safety evaluation:
placing the products prepared in application examples 1-9 and comparative application examples 1-5 in a patch tester, attaching the patch tester with the test object to the forearm flexor side of the tested person (20), and uniformly applying the patch to the skin by lightly pressing the palm for 24 hours; skin irritation and sensitization were observed as shown in Table 1 below 30min, 24h, 48h after removal of the subject plaque tester, respectively, and the observations were recorded.
TABLE 1
The results show that the products prepared in application examples 1-9 and comparative application examples 1-5 all have a rating of 0, which indicates that the skin barrier repair composition according to the present invention and the emulsion prepared therefrom are safe and non-irritating to the skin.
Test example 2
And (3) moisture preservation effect evaluation:
the instrument was the german model courage+ Khazaka electronic: derma UnitSSC, test temperature 25℃and humidity 60%. 150 skin-healthy 18-40 year old volunteers were selected, each half of men and women, randomly divided into 15 groups of 5 men and 5 women, using the products provided in application examples 1 to 9 and comparative application examples 1 to 5, respectively, and the blank group did not use any product. Each subject was tested for skin moisture content (in terms of 100% prior to use) three times before use and 5 hours after use, and after averaging, each group of averages was calculated with the decimal place remaining. The results are shown in Table 2.
TABLE 2
As can be seen from the data in table 2, the composition and the cosmetic prepared therefrom according to the present invention can repair skin barrier, prevent evaporation of water, help to strengthen and restore skin moisturizing state, and have remarkable synergistic effect on the above effects by recombinant collagen, conopeptide, schizophyllan and ectoin, and at the same time, the above effects of the product can be further improved by adding erigeron breviscapus extract and witch hazel extract.
Test example 3
Antiallergic performance evaluation:
the hyaluronidase in vitro inhibition assay Elson-Morgan was used. The sample solution group was prepared by taking 0.1mL of 0.25mmol/L CaCl 2 The solution and 0.5mL hyaluronidase solution are incubated for 20min at 37 ℃; 0.5mL of 10% diluted aqueous solution of the products of examples 1-3 and comparative examples 1-4 was added respectively, and incubation was continued for 20min at 37 ℃; adding 0.5mL sodium hyaluronate solution, maintaining the temperature at 37 ℃ for 30min, and standing at normal temperature for 5min; adding 0.1mL of 0.4mol/L NaOH solution and 0.5mL of acetylacetone solution, placing in a boiling water bath, heating for 15min, and immediately cooling with ice water for 5min; 1.0mL of the Escherichia reagent was added and diluted with 3.0mL of absolute ethanol, and the mixture was left for 20 minutes to develop a color, and the absorbance was measured by a spectrophotometer. The calculation formula is as follows: hyaluronidase inhibition ratio (%) = [ ((a-B) - (C-D))/a-B]X 100%, wherein: a represents the OD of the control solution (hyaluronidase + deionized water + sodium hyaluronate), B represents the OD of the control blank solution (acetate buffer + deionized water + acetate buffer), and C represents (hyaluronic acid)The OD value of the sample solution of the acid enzyme + sample + sodium hyaluronate), D represents the OD value of the sample blank solution of the (acetate buffer + sample + acetate buffer).
Each group of samples was tested in triplicate, and after averaging, the average value for each group was calculated, leaving the decimal place behind. The results are shown in Table 3.
TABLE 3 Table 3
Group of | Hyaluronidase inhibition (%) |
Example 1 | 68.4 |
Example 2 | 66.7 |
Example 3 | 63.9 |
Comparative example 1 | 66.8 |
Comparative example 2 | 68.0 |
Comparative example 3 | 57.3 |
Comparative example 4 | 51.2 |
As can be seen from the data in table 3, the composition according to the present invention has excellent hyaluronidase inhibiting effect, can pacify inflammatory reaction and other uncomfortable symptoms of skin, maintains stability and health of skin, and has remarkable synergistic effect of recombinant collagen, conopeptide, schizophyllan and exendin on the above effects.
Test example 4
Evaluation of anti-aging effect:
the instrument is a German Courage+Khazaka skin elasticity tester MPA-580, the test temperature is 25 ℃, and the humidity is 60%. Selecting 150 volunteers 18-40 years old with healthy skin, randomly dividing the volunteers into 15 groups of 5 men and 5 women, marking measuring areas on the inner sides of the forearms of the two hands of the subject, and performing experimental areas at least 3cm multiplied by 3cm, wherein the interval between each measuring area is at least 1cm. The products provided in application examples 1 to 9 and comparative application examples 1 to 5 were used, respectively, and the control group did not use any product. The 14 groups of samples are measured according to 2ml/cm 2 Single coating was performed (blank set is pure water). The test specimens were uniformly coated onto the test area using latex gloves and gently tapped until absorbed. Once daily, and after two weeks of consistent use, the results were determined. And vertically tapping the probe to the region to be tested to start testing, and removing the probe until the testing cycle is finished. Three points are taken for each region to be measured, and after the average value is taken, each group of average values are calculated. The results are shown in Table 4.
TABLE 4 Table 4
As can be seen from the data in table 4, the composition and the cosmetic prepared therefrom according to the present invention can repair skin barrier, prevent evaporation of water, help to strengthen and restore skin moisturizing state, and have remarkable synergistic effect on the above effects by recombinant collagen, conopeptide, schizophyllan and ectoin, and at the same time, the above effects of the product can be further improved by adding erigeron breviscapus extract and witch hazel extract.
The applicant states that the technical solution of the present invention is illustrated by the above embodiments, but the present invention is not limited to the above embodiments, i.e. it does not mean that the present invention must be implemented by the above embodiments. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Claims (10)
1. A skin barrier repair composition comprising recombinant collagen, wherein the skin barrier repair composition comprises recombinant collagen, conopeptides, schizophyllan and ectofactor.
2. The skin barrier repair composition containing recombinant collagen according to claim 1, wherein the skin barrier repair composition comprises, in parts by mass, 0.01-2 parts of recombinant collagen, 0.1-3 parts of conopeptide, 0.1-0.5 part of schizophyllan and 0.1-1 part of ectoin.
3. The skin barrier repair composition containing recombinant collagen according to claim 1 or 2, wherein the nucleotide sequence encoding the recombinant collagen is the sequence shown in SEQ ID No. 1.
4. Use of the skin barrier repair composition containing recombinant collagen according to any one of claims 1 to 3 in the preparation of a cosmetic.
5. The use according to claim 4, wherein the skin barrier repair composition comprising recombinant collagen is present in the cosmetic in an amount of 0.1 to 15% by mass.
6. An emulsion for strengthening skin barrier repair is characterized by comprising the following preparation raw materials in percentage by mass: the skin barrier repair composition containing recombinant collagen according to any one of claims 1 to 3, 0.1 to 15%, an oil phase 1 to 20%, an emulsifier 1 to 10% and water 50 to 90%.
7. The skin barrier repair enhancing emulsion of claim 6, wherein the oil phase comprises any one or a combination of at least two of glycerol caprylate, squalane, olive oil, white pool seed oil, jojoba oil, shea butter, sweet almond oil, grape seed oil, caprylic/capric triglyceride, hydrogenated cocoglyceride, phytosterol isostearate, isononyl isononanoate, arachidyl alcohol, vitamin a, vitamin E, or vitamin F; preferably, a combination of white pool flower seed oil, sweet almond oil, grape seed oil and vitamin E;
preferably, the emulsifier comprises any one or a combination of at least two of glycerol stearate, cetostearyl alcohol, sodium stearoyl lactylate, glycerol stearate citrate, cholesterol, cetostearyl alcohol oleyl oleate, sorbitan stearate, polyglycerol-10 oleate or polysorbate-60.
8. The skin barrier repair enhancing emulsion according to claim 6 or 7, wherein the emulsion is prepared from the following raw materials in mass percent: 1-15% of plant source conditioning agent, 0.1-2% of pH regulator, 0.01-0.5% of chelating agent, 0.05-0.5% of antioxidant and 0.1-0.5% of aromatic agent or the combination of at least two.
9. The emulsion for enhancing skin barrier repair of claim 8, wherein the plant-derived conditioning agent comprises witch hazel extract and/or erigeron breviscapus extract.
10. A method of preparing an emulsion for enhancing skin barrier repair according to any one of claims 6 to 9, wherein the method of preparing comprises the steps of:
(1) Mixing water and skin barrier repair composition at 25-40deg.C, homogenizing to obtain water phase; mixing the oil phase with emulsifier at 35-50deg.C, and homogenizing to obtain oil phase;
(2) Mixing and homogenizing the water phase, the oil phase and the rest preparation raw materials at 25-40 ℃ to obtain the emulsion for reinforcing skin barrier repair.
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