CN115413670A - Application of bactericidal composition in preventing and treating citrus resinosis - Google Patents

Application of bactericidal composition in preventing and treating citrus resinosis Download PDF

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CN115413670A
CN115413670A CN202211066295.3A CN202211066295A CN115413670A CN 115413670 A CN115413670 A CN 115413670A CN 202211066295 A CN202211066295 A CN 202211066295A CN 115413670 A CN115413670 A CN 115413670A
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pyraclostrobin
copper
sulfate
citrus
calcium
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张通
曹巧利
冯塔
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Shaanxi Meibang Pharmaceutical Group Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A01N59/20—Copper
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides

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Abstract

The invention relates to an application of a bactericidal composition in preventing and treating citrus resiniferosis, in particular to a bactericidal composition with pyraclostrobin and calcium copper sulfate as effective active ingredients, which is mainly characterized in that: the weight ratio of the pyraclostrobin to the copper calcium sulfate is 1-1 to 8, the composition is prepared into water dispersible granules, and the dosage of effective active ingredients is 500-1166.7 mg/kg.

Description

Application of bactericidal composition in preventing and treating citrus resinosis
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to an application of a bactericidal composition for preventing and treating citrus resiniferosis.
Background
Pyraclostrobin, chemical name N- [2- [ [1- (4-chlorophenyl) pyrazol-3-yl]Oxymethyl radical]Phenyl radical]-N-methoxy carbamic acid methyl ester of formula C 19 H 18 ClN 3 O 4 . Pyraclostrobin is strobilurin fungicide, can finally cause cell death by inhibiting mitochondrial respiration, and has the functions of protection, treatment and leaf penetration and conduction. The pyraclostrobin is mainly used for preventing and controlling various diseases caused by fungi on crops, and has good prevention and control effects on wheat powdery mildew and gibberellic disease. Pyraclostrobin, in addition to having a direct effect on pathogenic bacteria, has the ability to mutagenize physiological phenomena in many crops, especially grains, such as increasingThe absorption of nitrogen promotes the rapid growth of crops, improves the crop yield and achieves the aim of high crop yield.
Copper calcium sulfate, named English, is commonly used in plants to kill fungal diseases, has a certain toxicity, and is not directly contacted with human bodies. However, the amount and usage need to be careful because Cu is contained. It is a broad-spectrum protective bactericide, can be widely applied to fungal diseases on fruit trees and vegetables, and is used for preventing and treating canker, downy mildew and the like.
Citrus resinosis, also known as sand peel disease and stem rot disease, is a disease occurring in citrus caused by infection of citrus aschersonia. The main harm to branches, fruits, leaves and other parts can cause great fruit rot in the process of large-area garden destruction or storage and transportation when serious, and the service life of trees and the commodity value of fruits are influenced.
Citrus resiniferosis is one of the commonly occurring and serious harmful diseases in citrus producing areas in China. The 1 st peak of the disease occurs when the rain is more in 5-6 months. And in 9-10 months, the rainwater is increased again, and the disease is spread again. When suffering from freezing injury and waterlogging injury, the tree vigor is weak and the disease is serious. Extensive management, insufficient fertilizer supply, untimely or improper fertilization, water accumulation in the garden, serious hazards of longhorn beetles, gimeracil beetles and the like, and contribution to morbidity.
The inventor finds that the pyraclostrobin and the copper calcium sulphate which have different action mechanisms are prepared into the water dispersible granule composition according to a certain proportion, so that the citrus resiniferous disease can be effectively prevented, the prevention and treatment cost is reduced, the safety to crops is high,
disclosure of Invention
The invention aims to provide application of a bactericidal composition to control citrus resiniferosis.
The technical scheme of the invention is as follows:
the application of the bactericidal composition for preventing and treating citrus resiniferosis comprises the effective active ingredients of pyraclostrobin and calcium copper sulfate, wherein the weight ratio of pyraclostrobin to calcium copper sulfate is 1-1;
further, the bactericidal composition contains pyraclostrobin, calcium copper sulfate, a synergist, a white carbon black mixture and an auxiliary agent, and is prepared into water dispersible granules;
further, the total weight of the pyraclostrobin and the calcium copper sulfate in the bactericidal composition is 35-77%, and the preferred total weight is 70%;
furthermore, the dosage of the active ingredients of the sterilization composition is 500-1166.7 mg/kg, and the preferred dosage of the active ingredients is 700-1166.7 mg/kg.
Furthermore, the synergist is a mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol or alkoxy modified polytrisiloxane.
Furthermore, the auxiliary agent is selected from one or more of a dispersing agent, a wetting agent, a bonding agent, a defoaming agent, a pH regulator and a filler.
The dispersing agent is selected from one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, methylene dinaphthalene sodium sulfonate, lignin sodium salt or calcium salt, melamine formaldehyde resin, alkyl naphthalene sulfonate, EO-PO block polyether, styrene phenol polyoxyethylene ether phosphoric acid, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, dodecyl polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate, nonylphenol polyoxyethylene ether phosphate, naphthalenesulfonic acid formaldehyde condensate sodium salt block copolymer, comb-type polycarboxylate and sodium polycarboxylate;
the wetting agent is selected from one or more of sodium dodecyl benzene sulfonate, sodium diisobutylnaphthalene sulfonate, sorbitan fatty acid ester, sodium sulfosuccinate octyl ester, alkyl succinic acid sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, tristyrylphenol polyoxypropylene polyoxyethylene block polymer, sodium dodecyl sulfate, styrylphenol formaldehyde resin propylene oxide block polyether, castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkyl glycoside and fatty alcohol polyoxyethylene ether sodium sulfonate;
the binder is selected from one or more of water, starch, polyvinylpyrrolidone, polyethylene glycol, glucose and polyvinyl alcohol;
the pH regulator is selected from one or more of glacial acetic acid, citric acid, light calcium, potassium dihydrogen phosphate and triethanolamine;
the defoaming agent is selected from one or more of organic silicone, fatty alcohol and fatty acid;
the filler is selected from: one or more of white carbon black, calcined kaolin, ammonium sulfate, potassium sulfate, talcum powder, diatomite, starch and urea.
When the bactericidal composition is prepared into water dispersible granules, the bactericidal composition comprises the following components in percentage by weight: 38.9 to 385 grams of pyraclostrobin, 40.8 to 159.5 grams of calcium hydroxide, 183.8 to 718.5 grams of blue vitriod, 10 to 100 grams of synergist, 10 to 200 grams of dispersant, 10 to 100 grams of wetting agent, 1 to 100 grams of binder, 0 to 30 grams of defoamer, 0 to 40 grams of PH regulator and 1000 grams of filler.
The reaction formula of the bactericidal composition is as follows:
Ca(OH) 2 +Cu(SO 4 ) 2 ·5H 2 O→CuSO 4 ·3Cu(OH) 2 ·3CaSO 4 ·nH 2 O
preparing a certain amount of copper sulfate into an aqueous solution for later use, adding calcium hydroxide into a reaction kettle to prepare calcium hydroxide slurry, heating to 50-80 ℃, stirring for 0.5-1 hour, adding the prepared copper sulfate aqueous solution into the calcium hydroxide slurry, stirring for reaction for 1 hour, keeping the temperature for reaction for 4 hours, and obtaining the original copper sulfate calcium drug after the reaction is finished. And starting stirring, sequentially adding pyraclostrobin original drug, a dispersing agent, a wetting agent, a pH regulator, a synergist, a defoaming agent and a filler, shearing at a high speed for 20 minutes, performing 3-stage sanding to prepare a copper calcium sulfate and pyraclostrobin water dispersible granule intermediate, taking a small sample for detection, performing spray granulation after the detection is qualified, and performing secondary drying to prepare a finished product of the pyraclostrobin and copper calcium sulfate water dispersible granule.
Compared with the prior art, the bactericidal composition for preventing and treating citrus resiniferosis has the following beneficial effects: (1) Compared with a single agent, the composition has more remarkable effect on preventing and treating citrus resiniferosis and has obvious synergistic effect; (2) The two compounds with different action mechanisms are mixed, so that the generation of the drug resistance of diseases can be effectively delayed, the safety is good, and the citrus tree insecticide is safe; (3) The bactericidal composition has protection and treatment effects, can basically prevent diseases from occurring when used before the onset of disease, can effectively prevent and treat the diseases when used at the initial stage of the onset of disease, and has good persistence which can reach more than 20 days; (4) The pesticide has more effects, can effectively prevent and treat fungal diseases and bacterial diseases, and effectively reduces the pesticide cost of farmers; (5) Has the advantages of enhancing crop immunity, improving crop stress resistance and promoting crop growth.
Detailed Description
The present invention will be further described with reference to the following examples, which are not intended to limit the invention.
Application embodiment 1
Example 1 pyraclostrobin-copper calcium sulfate Water dispersible granule 70%
102 g of 98% pyraclostrobin original drug, 140 g of calcium hydroxide, 630.3 g of copper sulfate pentahydrate, 25 g of a mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 30 g of alkylphenol polyoxyethylene formaldehyde condensate sulfate, 20 g of alkyl polyoxyethylene ether sulfonate, 30 g of diisobutyl naphthalene sulfonate, 35 g of sorbitan fatty acid ester, 5 g of polyvinylpyrrolidone, 3 g of citric acid, 2 g of silicone defoamer, 30 g of ammonium sulfate and 1000 g of urea are added to prepare the 70% pyraclostrobin-copper sulfate calcium water dispersible granule.
Example 2 pyraclostrobin-copper calcium sulfate Water dispersible granule 70%
102 g of 98% pyraclostrobin original drug, 140 g of calcium hydroxide, 630.3 g of copper sulfate pentahydrate, 20 g of a mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 20 g of EO-PO block polyether, 25 g of styrene phenol polyoxyethylene ether phosphoric acid, 50 g of alkyl sulfate, 8 g of polyethylene glycol, 3 g of glacial acetic acid, 3 g of fatty alcohol, 20 g of white carbon black and 1000 g of starch are added to prepare the 70% pyraclostrobin-copper sulfate calcium water dispersible granule.
Example 3 pyraclostrobin-copper calcium sulfate Water dispersible granule 70%
102 g of 98% pyraclostrobin original drug, 140 g of calcium hydroxide, 630.3 g of copper sulfate pentahydrate, 30 g of alkoxy modified polytrisiloxane, 25 g of alkylphenol polyoxyethylene formaldehyde condensate phosphate, 35 g of sodium methylene dinaphthalene sulfonate, 40 g of triphenylethylphenol polyoxypropylene polyoxyethylene block polymer, 3 g of polyethylene glycol, 8 g of organic silicone defoamer, 10 g of monopotassium phosphate, 30 g of starch and 1000 g of urea are added to prepare the 70% pyraclostrobin-copper sulfate calcium water dispersible granule.
Example 4% pyraclostrobin-copper calcium sulfate Water dispersible granules
112 g of 98% pyraclostrobin original drug, 154 g of calcium hydroxide, 693.3 g of copper sulfate pentahydrate, 2 g of alkoxy modified polytrisiloxane, 30 g of alkylphenol polyoxyethylene ether sulfonate, 25 g of phenethyl phenol polyoxyethylene ether phosphate, 40 g of sodium dodecyl benzene sulfonate, 5 g of fatty alcohol, 2 g of polyvinyl alcohol, 2 g of citric acid and 1000 g of potassium sulfate to prepare the 77% pyraclostrobin-copper sulfate calcium water dispersible granule.
Example 5% pyraclostrobin-copper calcium sulfate Water dispersible granules
102 g of 98% pyraclostrobin original drug, 93.3 g of calcium hydroxide, 420.2 g of copper sulfate pentahydrate, 30 g of alkoxy modified polytrisiloxane, 30 g of alkyl naphthalene formaldehyde condensate sulfonate, 30 g of sodium polycarboxylate, 35 g of phenethyl phenol polyoxyethylene ether phosphate, 10 g of castor oil polyoxyethylene ether, 5 g of polyethylene glycol, 3 g of fatty acid, 30 g of starch and 1000 g of ammonium chloride are added to prepare the 50% pyraclostrobin-copper sulfate calcium water dispersible granule
Example 6% pyraclostrobin-copper calcium sulfate Water dispersible granules
71.4% pyraclostrobin original drug with the concentration of 98%, 130.6 g of calcium hydroxide, 588.2 g of copper sulfate pentahydrate, 50 g of a mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 30 g of sodium lignin salt or calcium salt, 35 g of alkyl naphthalene sulfonate, 40 g of alkylphenol formaldehyde resin polyoxyethylene ether, 3 g of polyvinylpyrrolidone, 5 g of monopotassium phosphate, 0.2 g of silicone defoamer, 20 g of white carbon black and 1000 g of starch, so that the 63% pyraclostrobin-copper sulfate calcium water dispersible granule is prepared.
Example 7% pyraclostrobin-copper calcium sulfate Water dispersible granules
51 g of 98% pyraclostrobin original drug, 70 g of calcium hydroxide, 315.1 g of copper sulfate pentahydrate, 30 g of mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 40 g of alkylphenol polyoxyethylene ether phosphate, 30 g of comb-type polycarboxylate, 50 g of sodium dodecyl sulfate, 30 g of alkyl glycoside, 10 g of glucose, 9 g of organic silicone defoamer, 8 g of citric acid, 40 g of white carbon black, 50 g of urea and 1000 g of potassium sulfate, so as to prepare the 35% pyraclostrobin-copper calcium sulfate water dispersible granule.
Application example two:
toxicity determination of pyraclostrobin and copper calcium sulfate mixture on citrus resinosis
The toxicity of pyraclostrobin and calcium sulphate and the proportion of 1:1, 1: 2, 1:4, 1:6 and 1:8 thereof in mixing on citrus resin pathogen is determined by adopting a drug-containing culture medium method according to the standards of NY/T1156.2-2006 and NY/T1156.6-2006, and the result shows that pyraclostrobin and calcium sulphate are mixed according to the above 5 proportions, and the synergistic effect on citrus resin pathogen is shown. The experimental procedure was as follows:
purpose of 1 test
Toxicity of the pyraclostrobin and the copper calcium sulfate and mixed combination thereof in different proportions on citrus resiniferous pathogens is measured indoors, synergy evaluation is performed, suitability of the pyraclostrobin and the copper calcium sulfate is determined, and scientific basis is provided for research and development of mixed agents of the pyraclostrobin and the copper calcium sulfate.
2 conditions of the test
2.1 test targets
Citrus resinifera (Phomopsis cytopsorella) is preserved and provided by the research center of pesticide bioassay of chemical research institute in Hunan province.
2.2 culture conditions
The culture conditions of the test target and the tested target are that the temperature is 25 +/-5 ℃, and the relative humidity is more than 5% of soil.
2.3 instrumentation
A beaker, a pipette, a measuring cylinder, a culture dish, a volumetric flask, an autoclave, a biochemical incubator and the like.
Design of the experiment
3.1 test Agents
Pyraclostrobin (pyraclosteron) 97% technical; copper calcium sulfate (copper calcium sulfate) 98% technical material.
3.2 drug concentration design and solution formulation
Pyraclostrobin solution: weighing 0.0100g pyraclostrobin 97% original drug in a sterilized small beaker, dissolving with 0.2mL DMF, adding 96.8mL of sterile water containing 0.1% Tween80 emulsifier, stirring well to prepare 100mg/L mother liquor, and then releasing to 50, 10, 5, 1, 0.5 and 0.1mg/L concentrations with sterile water containing 0.1% Tween80 emulsifier for later use;
copper calcium sulfate solution: weighing 0.0400g of 98% original drug of copper calcium sulfate by using a sterilized small beaker, dissolving by using 0.2mL of DMF, adding 94.8mL of sterile water containing 0.1% of Tween80 emulsifier, uniformly stirring to prepare 400mg/L mother solution, and diluting by using sterile water containing 0.1% of Tween80 emulsifier to 200, 100, 50, 25 and 12.5mg/L concentrations for later use;
each mixed solution: respectively taking 5, 3 and 2mL of 50mg/L pyraclostrobin solution, respectively adding the 50mg/L pyraclostrobin solution into 5, 10, 12, 18 and 16mL of 50mg/L copper calcium sulfate solution to prepare 50mg/L pyraclostrobin-copper calcium sulfate 1:1, 1: 2, 1:4, 1:6 and 1:8 solutions, and diluting the solutions into 10, 5, 1, 0.5 and 0.1mg/L concentrations for standby by using sterile water containing 0.1 percent Tween80 emulsifier;
control solution: 0.2mL of DMF plus 99.8mL of sterile water containing 0.1% of Tween80 emulsifier.
4 test method
According to the standard method of biological measurement NY/T1156.2-2006, a drug-containing culture medium method is adopted. 3mL of liquid medicine with each single dose and mixed dose series concentration is taken and added into 27mL of PDA culture medium cooled to 45 ℃ to prepare a medicine-containing culture medium plate with the required final concentration. Then, 6mm diameter hypha blocks were prepared from the edges of colonies of citrus resiniferous germ cultured for 10 days, and transferred to each series of drug-containing culture medium with the hypha facing downward, and the treatment was repeated 4 times. After the treatment, the culture medium is placed in a constant-temperature biochemical incubator at 28 ℃ for culture, the diameter of a bacterial colony is measured 7 days later, and the growth inhibition rate is calculated.
5 data investigation and statistical analysis
5.1 methods of investigation
And (5) measuring and recording the diameter of the disease spots according to the disease condition of the blank control, and calculating the control effect (%).
Figure BDA0003827800200000091
In the formula: i represents the hypha growth inhibition rate; d 0 Indicates the growing diameter of the blank control colony; dt represents the growth diameter of the agent-treated colony.
5.2 method for evaluating synergistic Effect
By referring to indoor bioassay test criteria NY/T1156.6-2006 for pesticides, the synergism of the mixed pesticide is evaluated according to a co-toxicity coefficient method (CTC) of Sun & Johnson (1960), namely CTC is not more than 80 as antagonistic action, 80-straw CTC-straw-type 120 is additive action, and CTC is not less than 120 as synergistic action.
Figure BDA0003827800200000101
Theoretical virulence index (TTI) = virulence index of agent A. Times% virulence index of agent A (%) + virulence index of agent B. RTM.
Figure BDA0003827800200000102
5.3 statistical analysis of data
All experimental data were analyzed using SAS6.12 statistical software. Hyphal growth inhibition rate (%) was calculated from the test data, and virulence regression equation, correlation coefficient (r) and EC50 (95% confidence limit) were calculated.
6 results analysis and discussion
6.1 evaluation of drug efficacy
The toxicity test results of the pyraclostrobin and the calcium copper sulfate mixed and combined in different proportions on the citrus resiniferous diseases are shown in the table 1. As can be seen from Table 1, pyraclostrobin and calcium copper sulfate mixed in the ratio of 1:1, 1: 2, 1:4, 1:6 and 1:8 have synergistic effect on citrus resina citri reticulatae germs, the EC50 values of the pyraclostrobin and calcium copper sulfate mixed in the ratio of 1:1, 1: 2, 1:4, 1:6 and 1:8 are respectively 0.29, 0.41, 0.60, 0.71 and 1.01mg/L, the co-toxicity coefficients of the pyraclostrobin and calcium copper sulfate mixed in the ratio of 1:6 are respectively 136.07, 144.14, 154.25, 177.52 and 153.21, and the mixing synergistic effect of the pyraclostrobin and the calcium copper sulfate mixed in the ratio of 1:6 is better.
TABLE 1 toxicity test results of pyraclostrobin and calcium copper sulfate mixture for citrus resinosis
Figure BDA0003827800200000111
6.2 discussion and conclusions
Indoor bioassay results show that the pyraclostrobin and the copper calcium sulfate are mixed according to the proportion of 1:1, 1: 2, 1:4, 1:6 and 1:8, and the pyraclostrobin and the copper calcium sulfate show synergistic effects on citrus resinifera pathogens, wherein the synergistic effect of 1:6 is the best.
Application example three: example 1 field efficacy experiment for controlling citrus resiniferosis
The test is carried out according to 'pesticide field efficacy test criteria' (III) 2004 'bactericide field efficacy test criteria for preventing and treating citrus resinifera' and 'pesticide field efficacy test standard operating procedure' of plant protection research institute of Guangxi Zhuang autonomous region academy of agricultural sciences 'standard operating procedure for preventing and treating citrus resinifera with bactericide field efficacy test standard operating procedure (SOP-F-079').
Test crop Citrus Tree
Controlling pitch sickness of a subject
1 basic information of the test
1.1 name of the test
The 70% pyraclostrobin-copper calcium sulfate water dispersible granule is used for field drug effect test on citrus resiniferous diseases.
1.2 purpose of the test
The control effect, the applicable dosage and the safety of the 70% pyraclostrobin-copper calcium sulfate water dispersible granule on citrus resinosis provided by the applicant are determined, and a basis is provided for registration and popularization of test medicaments.
2 environmental and facility cultivation conditions
2.1 test site
Guangxi Liuzhou city.
2.2 assay target cases
Citrus resiniferosis (Phomopsis cytopsorella)
2.3 test crops, varieties and growth conditions
The experimental crop is kumquat, and the test variety is oil soaked kumquat. Is a susceptible variety of citrus tree resinosis, and the citrus tree grows well.
3 Experimental design and arrangement
3.1 test dosage and numbering
TABLE 2 test design of test agents
Figure BDA0003827800200000121
3.2 methods of application
3.2.1 drug delivery period and method
The medicine is applied for 3 times in total, and the first application time is 2019, 05 and 15 days; the second application time is 05 and 25 months in 2019; the third application time is 2019, 06 and 04 months.
3.2.2 Capacity of use
Spraying, wherein the water consumption for application is 1125L/hectare.
3.3 investigation methods, time and frequency
3.3.1 investigation time and number of surveys
The test surveys 3 times, and the 1 st survey is a survey of the base number of diseases before application, the citrus reticulata resina (sand skin disease) does not occur yet, belongs to the early stage of the diseases, and the base number of the diseases is 0. The 2 nd investigation time is the investigation control effect on the 10 th day after the second drug application and is carried out on 4 days in 6 months. The 3 rd investigation time is 20 th day after the last application, namely 6 months and 24 days.
3.3.2 methods of investigation
2 plants are investigated in each cell, each plant is sampled according to 5 points in east, west, south and north, 2 branches are investigated in each point, and 4 leaves below the top leaf are investigated in each branch. Leaf (fruit) grading criteria:
no disease spot at level 0;
level 1: the lesion area accounts for less than 5% of the whole leaf area;
grade 3, the lesion area accounts for 6 to 10 percent of the whole leaf area;
grade 5, the lesion spot area accounts for 11 to 25 percent of the whole leaf area;
7 grade, the lesion area accounts for 26 to 50 percent of the whole leaf area;
and 9, stage: the lesion area accounts for more than 50% of the whole leaf area.
3.3.3 method for calculating drug effects
Figure BDA0003827800200000131
Figure BDA0003827800200000132
Note CK 0 -disease index of placebo zone; PT 0-disease index in treatment area.
4 results and analysis
TABLE 3 test result 1 of 70% pyraclostrobin-copper calcium sulfate water dispersible granule for controlling citrus resiniferous diseases
Figure BDA0003827800200000141
The survey results of 10 days after the 2 nd drug show that the average disease index of each drug treatment area is between 1.63 and 2.22, and the average disease index of the clear water control treatment area is 8.02. The control effects of the test agent 70% pyraclostrobin-copper calcium sulfate water dispersible granule 500 mg/kg (effective component, the same below), 700 mg/kg and 1166.7 mg/kg on citrus tree resinosis (sand skin disease) are respectively 74.03%, 76.63% and 79.68%, and the control agent 30% pyraclostrobin suspending agent 200 mg/kg and 77% copper calcium sulfate wettable powder 1540 mg/kg on citrus tree resinosis (sand skin disease) are respectively 73.60% and 72.27%. The control effect of the test medicament 500 mg/kg is not obviously different from that of the control medicament 1 and the control medicament 2; the control effect difference between the test medicament 700 mg/kg and the control medicament 1 is not significant, and the control effect difference between the test medicament 700 mg/kg and the control medicament 2 reaches a significant level of 5%; the control effect of the test medicament 1166.7 mg/kg is higher than that of the two control medicaments, and the control effect difference reaches a significant level of 1%.
The investigation result on the 20 th day after the 3 rd time of application shows that the control effects of the test medicament 70% pyraclostrobin-copper calcium sulfate water dispersible granule on citrus resinopathy (sand skin disease) are respectively 78.20%, 80.07% and 83.53% of the control medicament 70% pyraclostrobin-copper calcium sulfate water dispersible granule, 200 mg/kg of the control medicament 30% pyraclostrobin suspending agent and 1540 mg/kg of 77% copper calcium sulfate wettable powder on citrus resinopathy (sand skin disease), and are respectively 77.23% and 76.27%. The control effect of the test medicament 500 mg/kg is not obviously different from that of the control medicament 1 and the control medicament 2; the control effect difference between the test medicament 700 mg/kg and the control medicament 1 is not significant, and the control effect difference between the test medicament 700 mg/kg and the control medicament 2 reaches a significant level of 5%; the control effect of the test medicament 1166.7 mg/kg is higher than that of the two control medicaments, and the control effect difference reaches a 1% significant level.
Application example four: EXAMPLE 2 efficacy test for controlling Citrus resinifera
The third application example is referred to as specific experimental basis, experimental medicament and dosage and investigation method.
1 test site
San Ming City of Fujian province.
2 test crops, varieties and growth conditions
The citrus tree is a Wenzhou mandarin orange. The growth was good.
3 method of application
3.1 application periods and methods
The test is carried out in 2019, wherein the first application is carried out in 7-month and 15-day, the second application is carried out in 7-month and 22-day, and the third application is carried out in 7-month and 30-day; the application is carried out 3 times in total.
3.2 Capacity of use
The amount of the liquid medicine sprayed per hectare is about 1500 liters.
3.3 investigation time
Resin development was investigated before and 12 days after the 1 st application, respectively.
3.4 calculation method
Disease index = (number of leaf and fruit grains at each stage × representative value at each stage)/(total investigated leaf and fruit number × maximum representative value) x 100
The prevention and treatment effect (%) = [1- (disease index before drug treatment in control area x disease index after drug treatment in control area)/(disease index after drug treatment in control area x disease index before drug treatment in treatment area) ] × 100.
4 results and analysis
TABLE 4 test results of 70% pyraclostrobin-copper calcium sulfate water dispersible granule for preventing and treating citrus resinoid disease (12 days after the 3 rd time)
Figure BDA0003827800200000161
The test medicament 70% pyraclostrobin-copper calcium sulfate water dispersible granule in the embodiment 2 has 1400 times, 1000 times and 600 times of spraying application to the citrus tree resinosis (sand skin disease), the 600 times of prevention effect is superior to that of a control medicament 30% pyraclostrobin suspending agent 1500 times and 77% copper calcium sulfate wettable powder 500 times, the 1000 times of prevention effect is equivalent to that of the control medicament 30% pyraclostrobin suspending agent 1500 times, but is superior to that of the control medicament 77% copper calcium sulfate wettable powder 500 times, and the 1400 times of prevention effect is equivalent to that of the control medicament 77% copper calcium sulfate wettable powder 500 times. The 70% pyraclostrobin-copper calcium sulfate water dispersible granule has no adverse effect on the growth of oranges, and is safe after being used.
Application example five: EXAMPLE 3 efficacy test for controlling Citrus resinifera
The third application example is referred to as specific experimental basis, experimental medicament and dosage, investigation method and calculation method.
1 test site
A science and technology park of high and new agriculture of Jiangxi university of agriculture.
2 test crops, varieties and growth conditions
The citrus tree is a wide-peel citrus wenzhou mandarin orange (tail tension), and the citrus is subjected to flowering and then enters a fruit setting period during the test.
3 method of application
3.1 application periods and methods
The test is carried out on the first application in 5-month and 11-month days in 2019, the second application in 2-month and 6-month and the third application in 1-month and 7-month for 3 times.
3.2 Capacity of use
The amount of the liquid medicine sprayed per hectare is about 1350 liters.
3.3 investigation time
And performing a pharmacodynamic survey on the citrus tree before leaf blades 36 days after the 3 rd application.
4 results and analysis
TABLE 5 test results of 70% pyraclostrobin-copper calcium sulfate water dispersible granules for preventing and treating citrus resiniferous diseases
Figure BDA0003827800200000171
The result shows that the test medicament 70% of pyraclostrobin-copper calcium sulfate water dispersible granule in the embodiment 3 has a good control effect on citrus resinosis (sand skin disease). Before citrus resina disease occurs, namely after citrus is subjected to flower withering, the preparation enters a fruit setting period and is diluted by water by 600-1400 times, the effective component concentration is 500-1166.7 mg/kg of liquid medicine for spray control, then the preparation is applied for 2 times in a citrus young fruit period and a fruit expanding period, the application is performed for 3 times in the test, the leaf control effect investigation is performed 36 days after the 3-time application, the control effect of the test preparation on the young leaf resina disease (the sand skin disease) can reach 78.90-88.09%, and the control effect of the two high-dose treatments is remarkably higher than that of a control preparation 30% pyraclostrobin suspending agent diluted by 1500 times of water, namely the control effect of 200 mg/kg of effective component concentration treatment (79.35%) and that of another control preparation 77% copper calcium sulphate wettable powder preparation diluted by 500 times of water, namely the control effect of 1540 mg/kg of effective component concentration treatment (71.42%). The test medicament is safe to the growth of the citrus trees, has good concurrent effect on citrus canker and scab of the citrus trees, and is worthy of production, popularization and application.
The foregoing is a further detailed description of the invention in connection with specific preferred embodiments and it is not intended to limit the invention to the specific embodiments described. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.

Claims (8)

1. The application of the bactericidal composition in preventing and treating citrus resinoid diseases is characterized in that: the effective active ingredients of the bactericidal composition are pyraclostrobin and calcium copper sulfate, wherein the weight ratio of the pyraclostrobin to the calcium copper sulfate is (1) - (1).
2. Use according to claim 1, characterized in that: the bactericidal composition contains pyraclostrobin, calcium copper sulfate, a synergist, a white carbon black mixture and an auxiliary agent, and is prepared into water dispersible granules.
3. Use according to claim 1 or 2, characterized in that: the weight ratio of the pyraclostrobin to the copper calcium sulfate is 1.
4. Use according to claim 3, characterized in that: the weight ratio of the pyraclostrobin to the copper calcium sulfate is 1.
5. Use according to any one of claims 1 to 4, characterized in that: the total weight of the pyraclostrobin and the copper calcium sulfate in the bactericidal composition is 35% -77%.
6. Use according to claim 5, characterized in that: the total weight of the pyraclostrobin and the copper calcium sulfate in the bactericidal composition is 70%.
7. Use according to claim 2, characterized in that: the auxiliary agent is selected from one or more of dispersing agent, wetting agent, binder, defoaming agent, pH regulator and filler.
8. Use according to any one of claims 1 to 7, characterized in that: the dosage of the effective components of the sterilization composition is 500-1166.7 mg/kg.
CN202211066295.3A 2022-09-01 2022-09-01 Application of bactericidal composition in preventing and treating citrus resinosis Pending CN115413670A (en)

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