CN114487461A - TRUST verifying attachment - Google Patents
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- CN114487461A CN114487461A CN202210114163.7A CN202210114163A CN114487461A CN 114487461 A CN114487461 A CN 114487461A CN 202210114163 A CN202210114163 A CN 202210114163A CN 114487461 A CN114487461 A CN 114487461A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/571—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses for venereal disease, e.g. syphilis, gonorrhoea
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G01N2021/8411—Application to online plant, process monitoring
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/20—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
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Abstract
The invention discloses a TRUST (true single station test) testing device, which belongs to the technical field of medical testing equipment and comprises a testing table and an automatic liquid transferring mechanism; a reagent rack, a specimen rack, a gun head rack and a horizontal rotation instrument are arranged on the top surface of the inspection bench; the automatic liquid transfer mechanism comprises an electric liquid transfer device, a position adjusting device and a controller, wherein the position adjusting device is used for driving the electric liquid transfer device to move along the up-down direction, the front-back direction, the left-right direction and the left-right direction; the position adjusting device is arranged on the top surface of the inspection platform and is connected with the electric pipettor; the electric pipettor comprises a shell, a barrel, a pressure adjusting device and a separating device, wherein the barrel is used for connecting the pipette head, the pressure adjusting device is used for adjusting the air pressure in the barrel, and the separating device is used for separating the pipette head; the invention can automatically carry out the inspection operation, and solves the problem that an operator needs to manually add the reagent and move the liquid during the TRUST inspection operation.
Description
Technical Field
The invention relates to the technical field of medical inspection equipment, in particular to a TRUST inspection device.
Background
A toluidine red non-heating serum test (TRUST) is a non-specific treponema pallidum antigen serum test in a syphilis serum test, which uses cardiolipin, lecithin and cholesterol as antigens to check reactogens in serum for a preliminary screening test and a curative effect observation. At present, the TRUST test mainly comprises two detection methods, one is a qualitative test, the other is a quantitative test, the two methods both need operators to add reagents manually, particularly, the quantitative test needs to be diluted by times, and the pipetting operation needs to be carried out repeatedly, so that the workload of the operators is large, and great inconvenience is brought to the operators.
Disclosure of Invention
In view of the above, there is a need for a TRUST test device that solves the problem of requiring an operator to manually add reagents and pipette when performing a TRUST test operation.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a TRUST detection device comprises a detection platform and an automatic liquid-moving mechanism; a reagent rack, a specimen rack, a gun head rack and a horizontal rotation instrument are arranged on the top surface of the inspection table; the sample rack is provided with a plurality of sample grooves for placing samples to be detected, and the gun head rack is provided with a plurality of gun head grooves for placing pipette heads and a recovery groove for recovering the pipette heads; the top surface of the horizontal rotation instrument is provided with a plate groove for placing a TRUST check plate;
the automatic liquid transfer mechanism comprises an electric liquid transfer device, a position adjusting device and a controller, wherein the position adjusting device is used for driving the electric liquid transfer device to move along the up-down direction, the front-back direction, the left-right direction and the front-back direction; the position adjusting device is arranged on the top surface of the inspection platform and is connected with the electric pipettor; the electric pipettor comprises a shell, a gun tube, a pressure adjusting device and a separating device, wherein the gun tube is used for connecting the pipette tip, the pressure adjusting device is used for adjusting the air pressure in the gun tube, and the separating device is used for separating the pipette tip; the shell is connected with the position adjusting device, the gun barrel is fixedly connected with the shell, and the upper end of the gun barrel penetrates through the bottom of the shell; a piston is arranged on the inner side of the upper end of the gun barrel, and a lower limiting block and an upper limiting block are respectively arranged below and above the piston; the pressure adjusting device comprises a pressure chamber, the pressure chamber is connected with a negative pressure pump and a booster pump, and the pressure chamber is communicated with the upper port of the gun barrel; the separation device comprises a lower driving ring, an upper driving ring, a driving rod and a telescopic device; the lower driving ring is movably sleeved at the lower end of the gun barrel, and when the gun barrel is connected with the pipette tip, the pipette tip is close to the lower driving ring; the upper driving ring is positioned in the shell and movably sleeved on the gun barrel, the upper driving ring is connected with the lower driving ring through the driving rod, and the driving rod is movably connected with the shell; the upper driving ring is connected with the telescopic device, and the telescopic direction of the telescopic device is parallel to the length direction of the driving rod; the horizontal rotation instrument, the position adjusting device, the negative pressure pump, the booster pump and the telescopic device are electrically connected with the controller.
Preferably, the position adjusting device comprises a guide rail arranged on the top surface of the inspection bench, a movable frame arranged on the guide rail, a first driving device used for driving the movable frame to move back and forth on the guide rail, a lifting device arranged at the lower part of the movable frame and a second driving device used for driving the lifting device to move left and right on the movable frame; the shell of the electric pipettor is fixedly connected with the bottom of the lifting device; the first driving device, the second driving device and the lifting device are all electrically connected with the controller.
Preferably, the number of the guide rails is two, and the movable frame comprises two vertical rods and a horizontal rod which are vertically arranged; the bottom parts of the two upright posts are respectively connected with the two guide rails in a sliding manner through first sliding blocks, the two ends of the cross rod are respectively fixedly connected with the upper parts of the two upright posts, and the top part of the lifting device is connected with the cross rod in a sliding manner through second sliding blocks.
Preferably, the first driving device and the second driving device are both screw rod motors, and the lifting device and the telescopic device are both electric push rods.
Preferably, a feeding area, a gun head taking and placing area and a reaction area are arranged on the top surface of the inspection table from left to right; the reagent rack and the specimen rack are positioned in the feeding area, the gun head rack is positioned in the gun head taking and placing area, and the horizontal rotation instrument is positioned in the reaction area.
Preferably, the top surface of the level rotator is provided with a plurality of plate grooves.
Preferably, a photographing frame is arranged on the top surface of the inspection table, and the photographing frame is positioned outside the motion track of the position adjusting device; the photographic frame comprises a photographic plate and an L-shaped bracket which are horizontally arranged; one end of the L-shaped bracket is connected with the photographic plate, and the other end of the L-shaped bracket is connected with the inspection bench; the photographic plate is vertically opposite to the horizontal rotation instrument; the bottom surface of the photographic plate is provided with a plurality of high-definition cameras, and the high-definition cameras are in one-to-one correspondence with the plate grooves; the inspection table is also provided with a terminal machine which is electrically connected with the high-definition cameras; the high-definition camera is used for collecting image information after a specimen on the TRUST detection plate is reacted, and sending the image information to the terminal machine, and the terminal machine receives the image information and stores the image information; the terminal machine also compares the received image information with the pre-stored image information and makes result judgment according to the comparison condition.
Preferably, the terminal is a PC, and the controller is a single chip microcomputer or a PLC.
Due to the adoption of the technical scheme, the invention has the following beneficial effects:
1. the table top of the inspection table is provided with the reagent rack, the specimen rack and the gun head rack, and the reagent rack, the specimen rack and the gun head rack are used for positioning and fixing the inspection reagent, the specimen to be inspected and the liquid-transfering gun head, so that the reagent rack is conveniently matched with the automatic liquid-transfering mechanism, the operation of automatically adding the inspection reagent and the specimen to be inspected is realized, the workload of medical personnel is reduced through automatic operation, and the working efficiency is improved. Wherein, the position control device of automatic liquid-transfering mechanism can drive electronic pipettor and remove along upper and lower, preceding, back, left and right direction under the control action of controller to with electronic pipettor automatically regulated to the position of procedure setting, make electronic pipettor and corresponding inspection reagent, wait to examine sample, liquid-transfering gun head relative. The negative pressure pump and the booster pump of electronic pipettor are through the atmospheric pressure that changes the pressure chamber for the inboard atmospheric pressure in barrel upper end changes along with it, and the drive piston moves between last stopper and lower stopper, thereby changes the inside atmospheric pressure of liquid-transfering gun head, realizes that liquid-transfering gun head carries out ration imbibition, flowing back function automatically.
2. The invention can be used for qualitative test and quantitative test of TRUST test, can fully meet the basic requirements of TRUST test, and brings great convenience to operators. During operation, the reagents placed in the reagent rack are as follows: TRUST antigen suspension, positive control serum, negative control serum, and physiological saline. During qualitative test, the electric pipettor and the position regulating device are matched with each other under the control of the controller, 50 microliters of syphilis positive control, 50 microliters of syphilis negative control and 50 microliters of samples to be detected are respectively dripped into three circles of the TRUST test board in sequence, 50 microliters of TRUST antigen suspension is respectively dripped into the three circles, and finally the controller controls horizontal rotation transfer to drive the TRUST test board to rotate for 8 minutes at the speed of 100 revolutions per minute, so that the reaction result can be observed and judged. During quantitative test, the electric pipettor and the position adjusting device are matched with each other under the control of the controller, and 50 microliters of samples to be tested are respectively dripped into two circles of the TRUST test board; then, 50 microliters of physiological saline is respectively dripped into other circles; then, performing multiple dilution on the sample to be detected in one circle by using physiological saline through the automatic liquid transferring function of the automatic liquid transferring mechanism; then, 50 microliters of TRUST antigen suspension is respectively and automatically dripped into each circle; and finally, the controller controls horizontal rotation transfer to drive the TRUST check board to rotate for 8 minutes at the speed of 100 revolutions per minute, and then the reaction result can be observed and judged.
3. The electric pipettor has the function of automatically mounting and dismounting the pipette tip, is convenient for the electric pipettor to automatically replace the pipette tip before transferring different liquids, and avoids polluting reagents or samples. After the electric pipettor finishes pipetting, the controller firstly controls the position adjusting device to move the electric pipettor to the upper part of the recovery groove of the gun head frame; then, the controller controls the telescopic device to act, the upper driving ring pushes the lower driving ring to push out downwards through the driving rod, and therefore the pipette tips are pushed and dropped into the recovery tank; then, the controller controls the position adjusting device to move the electric pipettor to the position above the gun head groove of the gun head frame; and finally, the controller controls the lifting device to descend, so that the lower part of the barrel of the electric pipettor is connected with the pipette tips in the tip grooves, and the mounting operation of the pipette tips can be completed.
4. The test bed is provided with the photographic frame, the photographic frame is provided with the high-definition camera, the high-definition camera is used for collecting image information after a sample on the TRUST test board is reacted and sending the image information to the terminal, and the terminal receives and stores the image information, so that a data storage function is realized, and the subsequent operations of calling, printing and the like on data are facilitated. The terminal machine also compares the received image information with the pre-stored image information and makes result judgment according to the comparison condition. The inspection result is judged through the terminal machine, the workload of operators is further reduced, and the working efficiency of the operators is improved.
Drawings
FIG. 1 is a top view of a TRUST test device provided by an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of an automatic pipetting mechanism provided by an embodiment of the invention;
fig. 3 is a schematic structural diagram of an electric pipette provided in the embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a camera stand according to an embodiment of the present invention;
the main reference symbols in the drawings are as follows:
in the attached drawing, 1-inspection bench, 2-reagent rack, 3-reagent tank, 4-sample rack, 5-sample tank, 6-gun head rack, 7-gun head tank, 8-recovery tank, 9-horizontal rotator, 10-plate tank, 11-guide rail, 12-movable rack, 13-vertical rod, 14-cross rod, 15-first driving device, 16-lifting device, 17-second driving device, 18-electric pipettor, 19-shell, 20-gun tube, 21-piston, 22-lower limiting block, 23-upper limiting block, 24-pressure chamber, 25-negative pressure pump, 26-booster pump, 27-lower driving ring, 28-upper driving ring, 29-driving rod, 30-telescopic device, 31-photographic plate, 32-L-shaped bracket, 33-high-definition camera, 34-terminal machine, 35-TRUST check plate and 36-pipette tip.
The following detailed description will further illustrate the invention in conjunction with the above-described figures.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The drawings are only for purposes of illustration and are not intended to be limiting, certain elements of the drawings may be omitted, enlarged or reduced to better illustrate the embodiments of the present invention, and do not represent the size of the actual product, and it is understood that some well-known structures, elements and descriptions thereof in the drawings may be omitted for persons skilled in the art.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed" and "connected" are to be interpreted broadly, e.g., as a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Examples
As shown in fig. 1 to 4, a TRUST test apparatus includes a test stand 1 and an automatic pipetting mechanism. The top surface of the inspection bench 1 is provided with a reagent rack 2, a specimen rack 4, a gun head rack 6 and a horizontal rotator 9. The reagent rack 2 is provided with four reagent grooves 3 for placing the detection reagents, and the four reagent grooves 3 are respectively used for placing the following reagents: TRUST antigen suspension, positive control serum, negative control serum, and physiological saline.
The specimen holder 4 is provided with a plurality of specimen slots 5 for placing specimens to be detected. The gun head frame 6 is provided with a plurality of gun head grooves 7 for placing the pipette heads 36 and a recovery groove 8 for recovering the pipette heads 36. Pipette tips 36 are inserted into the tip grooves 7 for convenient use, and the recovery grooves 8 are used for recovering the used pipette tips 36. In order to ensure that reagents and specimens are not contaminated, the pipette tips 36 need to be replaced before transferring different liquids, and if liquid B needs to be transferred after liquid A is transferred, the pipette tips 36 need to be replaced before transferring liquid B, and the used pipette tips 36 are recovered in the recovery tank 8.
The top surface of the horizontal rotator 9 is provided with a plate groove 10 for placing the TRUST check plate 35, and the TRUST check plate 35 is fixed and limited by the plate groove 10, so that the position deviation of the TRUST check plate 35 when the TRUST check plate rotates along with the horizontal rotator 9 is avoided. Wherein, the top surface of the horizontal rotation instrument 9 is provided with a plurality of plate grooves 10, and a TRUST check plate 35 can be placed in each plate groove 10, so that a plurality of groups of tests can be simultaneously carried out. In this embodiment, the number of the plate grooves 10 is four.
Specifically, the top surface of the inspection bench 1 is provided with a feeding area, a gun head taking and placing area and a reaction area from left to right, the reagent rack 2 and the specimen rack 4 are located in the feeding area, the gun head rack 6 is located in the gun head taking and placing area, and the horizontal rotator 9 is located in the reaction area. The arrangement is clear in partition, orderly and orderly, and convenient to operate.
The automatic pipetting mechanism comprises an electric pipettor 18, a position adjusting device for driving the electric pipettor 18 to move along the up, down, front, back, left and right directions and a controller. The position adjusting device is installed on the top surface of the inspection station 1 and is connected with the electric pipette 18. By the adjusting action of the position adjusting device, the electric pipette 18 can be moved in the horizontal direction and the vertical direction at will, so that the electric pipette 18 is moved to a proper position.
The table top of the inspection table 1 is provided with the reagent rack 2, the specimen rack 4 and the gun head rack 6, which are used for positioning and fixing the inspection reagent, the specimen to be inspected and the pipette head 36, so that the inspection reagent, the specimen to be inspected and the pipette head can be conveniently matched with an automatic pipetting mechanism, the operation of automatically adding the inspection reagent and the specimen to be inspected is realized, the workload of medical personnel is reduced through automatic operation, and the working efficiency is improved. Because reagent groove 3 of reagent frame 2, sample groove 5 of sample frame 4, rifle head groove 7 and accumulator 8 of rifle head frame 6 and the board groove 10 of horizontal rotation appearance 9 all have fixed position coordinate, position control device can adjust electronic pipettor 18 to corresponding position to with the reagent in reagent groove 3, examine the sample in the sample groove 5, the sample is examined to the liquid-transfering rifle head 36 in rifle head groove 7, the TRUST inspection board 35 in accumulator 8 and the board groove 10 matches, rethread electronic pipettor 18's automatic imbibition and dropping liquid function, realize the automatic function of adding of reagent, sample.
The position adjusting device comprises a guide rail 11 arranged on the top surface of the inspection table 1, a movable frame 12 arranged on the guide rail 11, a first driving device 15 used for driving the movable frame 12 to move back and forth on the guide rail 11, a lifting device 16 arranged at the lower part of the movable frame 12 and a second driving device 17 used for driving the lifting device 16 to move left and right on the movable frame 12; the first driving device 15, the second driving device 17 and the lifting device 16 are all electrically connected with the controller. The number of the guide rails 11 is two, and the movable frame 12 comprises two upright rods 13 which are vertically arranged and a cross rod 14 which is horizontally arranged. The bottoms of the two upright posts 13 are respectively connected with the two guide rails 11 in a sliding manner through first sliding blocks, the two ends of the cross rod 14 are respectively fixedly connected with the upper parts of the two upright posts 13, and the top of the lifting device 16 is connected with the cross rod 14 in a sliding manner through second sliding blocks. In this embodiment, the first driving device 15 and the second driving device 17 are both screw motors, and the lifting device 16 is an electric push rod.
The electric pipettor 18 includes a housing 19, a barrel 20 for connecting a pipette tip 36, a pressure regulating device for regulating the air pressure inside the barrel 20, and a separating device for separating the pipette tip 36. The housing 19 is connected to the position adjusting device, in particular, the housing 19 is fixedly connected to the bottom of the lifting device 16. The barrel 20 is fixedly connected to the outer casing 19, and the upper end thereof penetrates through the bottom of the outer casing 19. A piston 21 is provided inside the upper end of the barrel 20, and a lower stopper 22 and an upper stopper 23 are provided below and above the piston 21, respectively. The pressure regulating means includes a pressure chamber 24, a negative pressure pump 25 and a pressurizing pump 26 are connected to the pressure chamber 24, and the pressure chamber 24 communicates with the upper port of the barrel 20.
The negative pressure pump 25 and the booster pump 26 change the air pressure in the pressure chamber 24, so that the air pressure in the inner side of the upper end of the gun barrel 20 is changed, the piston 21 is driven to move between the upper limiting block 23 and the lower limiting block 22, the internal air pressure of the pipette tip 36 is changed, and the automatic quantitative liquid suction and discharge functions of the pipette tip 36 are realized. In this example, the pipette tip 36 has a pipette tip volume of 50. mu.l per time.
The disengaging means comprise a lower drive ring 27, an upper drive ring 28, a drive rod 29 and a telescopic means 30. The lower drive ring 27 is movably mounted on the lower end of the barrel 20 and the pipette tip 36 is proximate to the lower drive ring 27 when the barrel 20 is connected to the pipette tip 36. An upper drive ring 28 is located within the housing 19 and movably mounted on the barrel 20, the upper drive ring 28 being connected to the lower drive ring 27 by a drive rod 29, the drive rod 29 being movably connected to the housing 19. The upper drive ring 28 is connected to a telescopic device 30, and the telescopic direction of the telescopic device 30 is arranged in parallel with the length direction of the drive rod 29. In this embodiment, the retractable device 30 is an electric push rod. The level rotator 9, the position adjusting device, the negative pressure pump 25, the booster pump 26 and the expansion device 30 are electrically connected to the controller.
The electric pipettor 18 has the function of automatically mounting and dismounting the pipette tip 36, so that the pipette tip can be conveniently and automatically replaced after the electric pipettor 18 transfers different liquids, and the pollution to reagents or samples is avoided. After the electric pipettor 18 finishes pipetting, the controller firstly controls the position adjusting device to move the electric pipettor 18 to the position above the recovery groove 8 of the gun head frame 6; then, the controller controls the telescopic device 30 to operate, the upper driving ring 28 pushes the lower driving ring 27 to push downwards through the driving rod 29, and the pipette tips 36 are pushed down into the recovery tank 8; then, the controller controls the position adjusting device to move the electric pipettor 18 to the position above the gun head groove 7 of the gun head frame 6; finally, the controller controls the lifting device 16 to descend, so that the lower part of the barrel 20 of the electric pipettor 18 is connected with the pipette tip 36 in the tip groove 7, and the tip installation operation can be completed.
In addition, the top surface of the inspection bench 1 is provided with a camera stand, and the camera stand is positioned outside the motion trail of the position adjusting device, so that the influence on the movement of the position adjusting device is avoided. The photographic frame comprises a photographic plate 31 and an L-shaped bracket 32 which are horizontally arranged, one end of the L-shaped bracket 32 is connected with the photographic plate 31, and the other end is connected with the inspection bench 1. The shooting plate 31 is vertically opposite to the horizontal rotation instrument 9, a plurality of high-definition cameras 33 are arranged on the bottom surface of the shooting plate 31, and the high-definition cameras 33 correspond to the plate grooves 10 one by one. A plurality of TRUST check boards 35 are monitored through a plurality of high definition cameras 33 respectively, the consistency of monitoring visual angles is ensured, and the accuracy is improved.
The inspection station 1 is further provided with a terminal machine 34, and the terminal machine 34 is electrically connected with the plurality of high-definition cameras 33. The high-definition camera 33 is used for collecting image information after the sample on the TRUST inspection board 35 is reacted, and sending the image information to the terminal 34, and the terminal 34 receives the image information and stores the image information; the terminal 34 also compares the received image information with the pre-stored image information, and makes a result judgment according to the comparison condition. The terminal 34 is a PC, and the controller is a single chip or a PLC. In this embodiment, the controller is a PLC.
According to the invention, the test bed 1 is provided with the photographic frame, the photographic frame is provided with the high-definition camera 33, the high-definition camera 33 is used for collecting image information after a specimen on the TRUST test board 35 is reacted and sending the image information to the terminal 34, the terminal 34 receives the image information and stores the image information, a data storage function is realized, and the subsequent operations of calling, printing and the like on data are facilitated. The terminal 34 also compares the received image information with the pre-stored image information, and makes a result judgment according to the comparison condition. The terminal machine 34 is used for judging the inspection result, so that the workload of the operator is further reduced, and the working efficiency of the operator is improved.
The invention can be used for qualitative test and quantitative test of TRUST test, can fully meet the basic requirements of TRUST test, and brings great convenience to operators. During operation, the reagent placed in the reagent rack 2 comprises: TRUST antigen suspension, positive control serum, negative control serum, and physiological saline.
During qualitative test, the electric pipettor 18 and the position adjusting device are matched with each other under the control of the controller, 50 microliters of syphilis positive control, 50 microliters of syphilis negative control and 50 microliters of samples to be tested are respectively dripped into three circles of the TRUST test plate 35 in sequence, 50 microliters of TRUST antigen suspension is respectively dripped into the three circles, and finally the controller controls horizontal rotation transfer to drive the TRUST test plate 35 to rotate at the speed of 100 revolutions per minute for 8 minutes, so that the reaction result can be observed and judged.
During quantitative test, the electric pipettor 18 and the position adjusting device are matched with each other under the control of the controller, and 50 microliters of samples to be tested are respectively dripped into two circles of the TRUST test board 35; then, 50 microliters of physiological saline is respectively dripped into other circles (or a specified number of circles); then, performing multiple dilution on the sample to be detected in one circle by using physiological saline through the automatic liquid transferring function of the automatic liquid transferring mechanism; then, 50 microliters of TRUST antigen suspension is respectively and automatically dripped into each circle; and finally, the controller controls horizontal rotation transfer to drive the TRUST check plate 35 to rotate at the speed of 100 revolutions per minute for 8 minutes, and then the reaction result can be observed and judged.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (8)
1. A TRUST verifying attachment, characterized by: comprises a test platform and an automatic liquid-moving mechanism; a reagent rack, a specimen rack, a gun head rack and a horizontal rotation instrument are arranged on the top surface of the inspection table; the sample rack is provided with a plurality of sample grooves for placing samples to be detected, and the gun head rack is provided with a plurality of gun head grooves for placing pipette heads and a recovery groove for recovering the pipette heads; the top surface of the horizontal rotation instrument is provided with a plate groove for placing a TRUST check plate;
the automatic liquid transfer mechanism comprises an electric liquid transfer device, a position adjusting device and a controller, wherein the position adjusting device is used for driving the electric liquid transfer device to move along the up-down direction, the front-back direction, the left-right direction and the front-back direction; the position adjusting device is arranged on the top surface of the inspection platform and is connected with the electric pipettor; the electric pipettor comprises a shell, a gun tube, a pressure adjusting device and a separating device, wherein the gun tube is used for connecting the pipette tip, the pressure adjusting device is used for adjusting the air pressure in the gun tube, and the separating device is used for separating the pipette tip; the shell is connected with the position adjusting device, the gun barrel is fixedly connected with the shell, and the upper end of the gun barrel penetrates through the bottom of the shell; a piston is arranged on the inner side of the upper end of the gun barrel, and a lower limiting block and an upper limiting block are respectively arranged below and above the piston; the pressure adjusting device comprises a pressure chamber, the pressure chamber is connected with a negative pressure pump and a booster pump, and the pressure chamber is communicated with the upper port of the gun barrel; the separation device comprises a lower driving ring, an upper driving ring, a driving rod and a telescopic device; the lower driving ring is movably sleeved at the lower end of the gun barrel, and when the gun barrel is connected with the pipette tip, the pipette tip is close to the lower driving ring; the upper driving ring is positioned in the shell and movably sleeved on the gun barrel, the upper driving ring is connected with the lower driving ring through the driving rod, and the driving rod is movably connected with the shell; the upper driving ring is connected with the telescopic device, and the telescopic direction of the telescopic device is parallel to the length direction of the driving rod; the horizontal rotation instrument, the position adjusting device, the negative pressure pump, the booster pump and the telescopic device are electrically connected with the controller.
2. A TRUST test device according to claim 1, wherein: the position adjusting device comprises a guide rail arranged on the top surface of the inspection bench, a movable frame arranged on the guide rail, a first driving device used for driving the movable frame to move back and forth on the guide rail, a lifting device arranged at the lower part of the movable frame and a second driving device used for driving the lifting device to move left and right on the movable frame; the shell of the electric pipettor is fixedly connected with the bottom of the lifting device; the first driving device, the second driving device and the lifting device are all electrically connected with the controller.
3. An automated syphilis testing device as claimed in claim 1, wherein: the number of the guide rails is two, and the movable frame comprises two vertical rods and a horizontal rod which are vertically arranged; the bottom parts of the two upright posts are respectively connected with the two guide rails in a sliding manner through first sliding blocks, the two ends of the cross rod are respectively fixedly connected with the upper parts of the two upright posts, and the top part of the lifting device is connected with the cross rod in a sliding manner through second sliding blocks.
4. An automated syphilis testing device as claimed in claim 3, wherein: the first driving device and the second driving device are both screw rod motors, and the lifting device and the telescopic device are both electric push rods.
5. A TRUST test device according to claim 1, wherein: a feeding area, a gun head taking and placing area and a reaction area are arranged on the top surface of the inspection table from left to right; the reagent rack and the specimen rack are positioned in the feeding area, the gun head rack is positioned in the gun head taking and placing area, and the horizontal rotation instrument is positioned in the reaction area.
6. A TRUST test device according to claim 1, wherein: the top surface of the horizontal rotation instrument is provided with a plurality of plate grooves.
7. A TRUST test device according to claim 6, wherein: a photographic frame is arranged on the top surface of the inspection table and is positioned outside the motion track of the position adjusting device; the photographic frame comprises a photographic plate and an L-shaped bracket which are horizontally arranged; one end of the L-shaped bracket is connected with the photographic plate, and the other end of the L-shaped bracket is connected with the inspection bench; the photographic plate is vertically opposite to the horizontal rotation instrument; the bottom surface of the photographic plate is provided with a plurality of high-definition cameras, and the high-definition cameras are in one-to-one correspondence with the plate grooves; the inspection table is also provided with a terminal machine which is electrically connected with the high-definition cameras; the high-definition camera is used for collecting image information after a specimen on the TRUST detection plate is reacted, and sending the image information to the terminal machine, and the terminal machine receives the image information and stores the image information; the terminal machine also compares the received image information with the pre-stored image information and makes result judgment according to the comparison condition.
8. A TRUST test device according to claim 7, wherein: the terminal machine is a PC, and the controller is a singlechip or a PLC.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210114163.7A CN114487461A (en) | 2022-01-30 | 2022-01-30 | TRUST verifying attachment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210114163.7A CN114487461A (en) | 2022-01-30 | 2022-01-30 | TRUST verifying attachment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114487461A true CN114487461A (en) | 2022-05-13 |
Family
ID=81478736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210114163.7A Pending CN114487461A (en) | 2022-01-30 | 2022-01-30 | TRUST verifying attachment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114487461A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116027036A (en) * | 2022-10-08 | 2023-04-28 | 合肥金域医学检验实验室有限公司 | Syphilis antibody detection and interpretation equipment and detection and interpretation method thereof |
-
2022
- 2022-01-30 CN CN202210114163.7A patent/CN114487461A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116027036A (en) * | 2022-10-08 | 2023-04-28 | 合肥金域医学检验实验室有限公司 | Syphilis antibody detection and interpretation equipment and detection and interpretation method thereof |
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