CN114258884B - Land-based intensive culture pond and culture method for sipunculus nudus - Google Patents

Abstract

The invention discloses a land-based intensive culture pond for a delicious euglena, which is divided into a water quantity control area and a culture area; a culture cave bed is movably arranged in the culture area; the culture cave bed comprises a culture rack and substrate cups, wherein the culture rack is provided with a plurality of substrate cups; wherein the water quantity control area supplies water quantitatively to the cultivation area; and the cultivation area is provided with drainage. And a cultivation method adopting the land-based intensive cultivation pool for the sipunculus nudus. The survival rate of the cultured delicious sipunculus nudus is more than 82.8%, the growth speed is high, the culture time is shortened by more than 1/3, the collection is convenient, the labor force is reduced, the economic benefit of the culture of the delicious sipunculus nudus is improved, the uncontrollable condition of the culture of the delicious sipunculus nudus on the beach is changed, the automation and industrialization of the culture of the delicious sipunculus nudus are realized, and the method has a very good application prospect.

Description

Land-based intensive culture pond and culture method for sipunculus nudus

Technical Field

The invention relates to a land-based intensive culture pond for a pungent sipunculus nudus and a culture method.

Background

Delicious euglenaPhascolosoma esculenta) Also commonly called as diced mud, mud worms, soil nails, soil shoots and the like, belonging to the phylum Sipunculus zoon, class Sipunculus, family Sipunculaceae, class Sipunculus grapunculusThe body is long and cylindrical, the kissing part is slender like a nail, the epidermis is distributed with tiny mastoid, the skin mainly inhabits in the intertidal zone, and the living is in the camp and living, and the food is eaten by organic chips, benthic diatoms and the like. The mud Ding Rouzhi is crisp and sweet, delicious in taste and rich in nutrition, contains various amino acids and trace elements required by a human body and taurine for regulating the functions of the human body, is commonly used for cooking porridge and soup, nourishing the body and is called as a 'ginseng in sea', and is a famous seafood. Therefore, the development of the proliferation and cultivation of the delicious sipunculus nudus has important practical significance.

At present, the culture land for the delicious sipunculus nudus is mainly prepared by ditching and ridging coastal beach, ponds, salty acid fields and the like, and then culturing the delicious sipunculus nudus on the ridge surface, but the culture environment of the culture land is difficult to manually regulate and control, so that the yield obtained by culturing is relatively low.

The cultivation method adopted on the cultivation land comprises the following steps: and (3) putting 800-1000 of the sarcandra glabra fries per kg on the ridge surface for cultivation, wherein the cultivation period is 1.5 years, and the cultivation survival rate is 50-65%.

During the cultivation process, some problems of the cultivation land are found:

(1) Because the delicious euglena camping living in a hole can grow well without being destroyed, the problems of dry dew, immersion and soil texture are related, and the areas of the beach, the pond and the salty acid field which meet the cultivation conditions are limited;

(2) The cultivation modes of ditching and ridging on the cultivation ground are greatly affected by weather and waves, the cultivation environment is not well controlled, and the hole of the delicious euglena is often damaged;

(3) When bait is thrown, the bait is lost along with water in a large amount due to the flowing of water in a farm or too deep water;

(4) The environment enemies such as beach, pond and salty acid field are more, and the survival rate of the culture of the delicious euglena is low;

(5) During harvesting, only hoe and shoveling modes can be adopted, so that the workload is high, and the damage to the delicious euglena can not be avoided, and the yield and the benefit are affected.

Therefore, the development of the culture industry of the sipunculus nudus is restricted by the existing artificial culture of the sipunculus nudus, a set of technology for carrying out system transformation and culture management on culture facilities is to be explored, the growth speed and survival rate of the sipunculus nudus are improved, and the method has important economic significance for the culture industry of the sipunculus nudus.

Disclosure of Invention

The invention aims at overcoming the defects of the prior art and provides a land-based intensive culture pond and a culture method for the delicious sipunculus nudus.

In order to achieve the above object of the present invention, the following technical scheme is adopted:

an intensive land-based culture pond for the sipunculus nudus, wherein the culture pond is divided into a water quantity control area and a culture area; a culture cave bed is movably arranged in the culture area; the culture cave bed comprises a culture rack and substrate cups, wherein the culture rack is provided with a plurality of substrate cups; wherein the water quantity control area supplies water quantitatively to the cultivation area; and the cultivation area is provided with drainage.

Further, the invention relates to an intensive land-based culture pond for the sipunculus nudus, which also comprises a second liquid level meter, a water drain valve, a drain valve and a controller; the water quantity control area is connected with the cultivation area through a water drain valve; the cultivation area is provided with a second liquid level meter and a drain valve; the controller is arranged in the water quantity control area or the cultivation area; and the second liquid level meter, the water drain valve and the water drain valve are all electrically connected with the controller.

Further, the invention relates to a land-based intensive culture pond for the sipunculus nudus, which also comprises a mud guard; the mud guard separates the cultivation area into a drainage area and a cultivation cave bed area, and the drainage valve is arranged in the drainage area; wherein, the distance between the mud guard and the drain valve is 10-12 cm, and the height is 5-7 cm.

Further, the invention relates to an intensive land-based culture pond for the sipunculus nudus, which further comprises a first liquid level meter, a water inlet pipe and a water inlet valve; the water quantity control area is provided with a first liquid level meter and a water inlet pipe; the water inlet pipe is provided with a water inlet valve; wherein, first level gauge, water intaking valve all are connected with the controller electricity.

The method for cultivating the delicious euglena by utilizing the land-based intensive cultivation pool for the delicious euglena comprises the following steps of;

(1) And (3) a culture pond: building a long box type cultivation pond with the width of 1.0-1.5 m and the depth of 40-50 cm, wherein the area of the pond is 1-100 square meters;

(2) Culture substrate: the dry dew is prepared from coarse sand, medium sand, fine powder sand and organic matters, and the humidity is kept at 25-45% per day;

(3) And (3) arranging a culture pond: adopting a land leveling arrangement method, wherein the distance between the culture ponds is 30-50 cm; or, adopting shelf type arrangement, wherein the superposition distance between the culture ponds is 60-80 cm;

(4) Preparation before stocking: filling the culture substrate in the step (2) into each substrate cup of the culture cave bed, compacting, putting the culture cave bed into a culture area, supplying water to the culture area from a water quantity control area, and adjusting water inlet and drainage operation by setting the water level to be controlled by submerging the culture cave bed by more than 10 cm;

(5) And (5) stocking seedlings: the seedlings with light color or gray black body color, neat specification, no damage to body surface, strong vitality and free osculating and stretching are selected for stocking, the stocking specification is 800-900 tails/-kg (the number of the delicious euglena is per 1kg, the same shall apply below), the stocking density is 1 tail/cup, and the seedling is placed by adopting the methods of draining water, digging out the substrate, placing the seedling in the substrate cup and covering the substrate;

(6) And (3) cultivation management: the sea tide phenomenon is imitated, the water is fed and discharged for 1 time every day, the immersion time of the culture cave bed is 12-16 hours, and the dry exposure time is 8-12 hours; feeding algae, fish meal or bean pulp powder for 1 time every day; culturing to 200-260 tails/kg, taking out the breeding hole bed from the culture area, turning upside down, pouring out the matrix, and harvesting the delicious euglena.

Further, the water quantity control area of the culture pond in the step (1) occupies 1/10-1/4 of the area of the culture pond, and is reserved to the required water level; a plurality of substrate cups are arranged on the culture cave bed in the culture area, and holes with the diameter of 10-12 mm are formed in the cup bottoms.

Further, the culture medium in the step (2) is mainly prepared from the following raw materials in parts by weight: 15-20 parts of coarse sand with the grain size of 0.5-2 mm, 15-25 parts of medium sand with the grain size of 0.25-0.5 mm, 30-50 parts of fine sand with the grain size of 0.016-0.25 mm, 5-20 parts of fine powder sand with the grain size of 0.004-0.016 mm and 5-10 parts of organic matters.

Further, in the step (4), the control water level is adjusted to perform water feeding and discharging operations: when water is fed, the cultivation area stops draining water outwards, the water quantity control area supplies water to the cultivation area, the water level in the cultivation area rises and submerges the cultivation cave bed, the cultivation cave bed is submerged to a set control water level, and the water quantity control area stops supplying water to the cultivation area; then, the cultivation area drains outwards, and the water level drops to expose the cultivation cave bed.

Further, the method of draining water, digging up the substrate, placing seedlings in a substrate cup and covering the substrate in the step (5): draining pond water in a culture area, digging 1/4-1/3 of matrixes in a matrix cup, then placing the matrixes in a matrix cup of the delicious sipunculus nudus Miao Yu according to 1 tail/cup, covering the digging matrixes back and covering the matrixes with the delicious sipunculus nudus seedlings, observing for 2-3 days, and after the delicious sipunculus nudus seedlings form a cave in the matrix cup, feeding water to the culture area by a water quantity control area to a set water level; during this period, the artemia salina seedlings were replaced if they were to drill out the substrate cup.

Further, the managing of the cultivation in the step (6) includes the following steps:

(1) and (3) water inlet and drainage: according to seasons, weather and growth conditions of the delicious euglena, simulating ocean tide phenomenon, feeding and draining water 1 time a day, wherein the culture water level in spring and winter is 10 cm above the culture cave bed, the culture water level in summer, autumn or rainy days is 20cm above the culture cave bed, the immersion time of the culture cave bed is 12-16 hours, the dry dew time is 8-12 hours, and the soil humidity is kept 25-45% per day;

(2) feeding: algae, fish meal or bean pulp powder is fed for 1 time every day, and the feeding method comprises the following steps: before water enters the cultivation area, algae, fish meal or bean pulp powder is put in a water quantity control area, and when water enters the cultivation area from the water quantity control area, the algae, the fish meal or the bean pulp powder enters the cultivation area through water flowing;

(3) and (3) patrol: checking for weather and facility operation conditions, checking for ingestion, growth and disease conditions of the delicious sipunculus nudus, making shading and storm prevention measures, controlling the salinity of the water for cultivation to be 7-30 per mill, the PH value to be 7.5-8.5, the dissolved oxygen to be more than 5 per liter, and keeping the humidity of the matrix dry dew to be 25-45 per day; measuring body length and body weight 1 time every 30 days to adjust bait feeding scheme;

(4) harvesting: culturing the delicious sipunculus nudus to 200-260 tails/kg, draining pool water, taking out the culture cave bed from the culture area, turning upside down, pouring out the matrix, digging out and dispersing the matrix, and searching for all the collected delicious sipunculus nudus; after harvesting, the substrate is exposed for more than 15 days and then returned to the substrate cup.

Compared with the prior art, the invention has the advantages that:

1. the culture pond can realize land-based culture of the delicious euglena, a plurality of culture ponds can be arranged on a flat ground or vertically overlapped and installed on a shelf, the culture pond is divided into a water quantity control area and a culture area, the water quantity control area is matched with the culture area to realize the ocean tide-like phenomenon, the environment of the culture area can be adapted to the delicious euglena, and the growth and development of the delicious euglena are promoted.

2. The method has the advantages of simple construction and installation of the culture pond, convenient maintenance, accurate and easily controlled culture feeding and water quality management, high growth speed, shortening of the culture time by more than 1/3, convenient collection and capture, labor force reduction and improvement of the economic benefit of the culture of the delicious euglena.

The method can prevent the organisms of the sipunculus nudus from damaging the fish, the shrimp, the crab, the bird and the like from entering the culture pond, the dry dew, the immersion time and the soil humidity of the culture field can be regulated and controlled to be optimal according to the culture condition, and compared with the beach, pond and salty acid field culture method, the culture environment is stable, the normal growth of the sipunculus nudus is facilitated, the survival rate of the sipunculus nudus is more than 82.8 percent, and is more than 17 percent higher than that of the prior culture technology.

4. According to the method, the inhabited soil of the delicious euglena is analyzed, and the unified culture medium is optimally prepared according to the influence of the dry exposure and immersion time on the cavern of the delicious euglena, so that the inhabitation and ingestion of the delicious euglena are facilitated, the influence of different soil textures and different humidity is avoided, and the specifications of the cultured delicious euglena are tidy.

5. The method adopts the mode of harvesting the delicious euglena by pouring the matrix, changes the traditional harvesting mode of digging by using tools such as a hoe, a shovel and the like, avoids the death injury of the delicious euglena, and improves the cultivation benefit.

6. The method expands the culture space of the sipunculus nudus, enables the sipunculus nudus culture to be moved from beach and pond to land-based culture, provides a technical method for realizing automation and industrialization of the sipunculus nudus culture, and has a very good application prospect.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. Like elements or portions are generally identified by like reference numerals throughout the several figures. In the drawings, elements or portions thereof are not necessarily drawn to scale.

FIG. 1 is a schematic view of a culture pond according to the present invention;

FIG. 2 is a schematic diagram of a cultivation structure according to the present invention;

the names and serial numbers of the components in the figure: 1-water quantity control area, 2-first liquid level meter, 3-inlet tube, 4-inlet valve, 5-water drain valve, 6-cultivation area, 7-cultivation cave bed, 71-cultivation frame, 72-matrix cup, 8-second liquid level meter, 9-drainage area, 10-drain valve, 11-fender, 12-controller.

Detailed Description

In order to make the technical solutions of the present application better understood by those skilled in the art, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings and examples, and it is apparent that the described examples are only a part of examples of the present application, and all other examples obtained by those skilled in the art without making any inventive effort are intended to be within the scope of protection of the present application.

Example 1:

as shown in fig. 1, an intensive culture pond of the land-based of the pungent glomerata, wherein the culture pond is divided into a water quantity control area 1 and a culture area 6; a culture cave bed 7 is movably arranged in the culture area 6; the culture cave bed 7 comprises a culture rack 71 and substrate cups 72, wherein the culture rack 71 is provided with a plurality of substrate cups 72; wherein, the water quantity control area 1 supplies water quantitatively to the cultivation area 6; the cultivation area 6 is provided with drainage.

The substrate cup can be a closed hollow square body with the following specifications: the upper opening is 50 mm multiplied by 50 mm, the bottom is 30 mm multiplied by 30 mm, and the height is 150 mm; the upper opening is 60 mm multiplied by 60 mm, the bottom is 30 mm multiplied by 30 mm, and the height is 150 mm; the upper opening is 50 mm×60 mm, the bottom is 30 mm×30 mm, the height is 150 mm, or the upper opening is 55 mm×55 mm, the bottom is 30 mm×30 mm, the height is 150 mm, etc.

The substrate cup can also be in a cylindrical structure, and has an upper opening phi of 50 mm, a bottom phi of 30 mm, a height of 150 mm, an upper opening phi of 55 mm, a bottom phi of 30 mm, a height of 150 mm or an upper opening phi of 60 mm, a bottom phi of 30 mm, a height of 150 mm and the like.

The working mode is as follows:

the water quantity control area stores water to a required water level in advance, so that the water quantity control area can supply water to the cultivation area in time.

The water quantity control area and the culture area are combined, so that the ocean tide imitating phenomenon can be realized conveniently. The water quantity control area supplies water to the cultivation area, after the cultivation cave bed is submerged to the set water level, the water supply is stopped, the cultivation cave bed is submerged and soaked by the water, when the cultivation cave bed is soaked to the required time, the water in the cultivation area can be discharged outwards, the water is discharged to the cultivation cave bed to expose the water surface, the water is kept dry and exposed to the required set time, then the water quantity control area supplies water to the cultivation area, after the cultivation cave bed is submerged to the set water level, the water supply is stopped, and the cultivation cave bed is re-soaked in the water, so that the circulation is repeated.

The culture pond can enable the land-based intensive culture of the delicious sipunculus nudus, overcomes the defects of the prior art, is accurate and easy to control in water quality management, is high in growth speed, shortens the culture time by more than 1/3, is convenient to collect and reduce labor force, and improves the economic benefit of the culture of the delicious sipunculus nudus.

Example 2:

compared with example 1, the difference is that: in order to realize water drainage and water discharge conveniently, a second liquid level meter 8, a water drain valve 5, a drain valve 10 and a controller 12 are added.

The water quantity control area 1 is connected with the cultivation area 6 through a water drain valve 15; the culture area 6 is provided with a second liquid level meter 8 and a drain valve 10; the controller 12 is arranged in the water quantity control area 1 or the cultivation area 6; wherein, the second liquid level meter 8, the water drain valve 5 and the water drain valve 10 are all electrically connected with the controller 12.

The working mode is as follows:

the controller 12 sets the soaking time and the dry dew time of the cultivation area, and the second liquid level meter 8 monitors the water level of the cultivation area, and can set the soaking water level and the dry dew water level. The controller coordinates and controls the water drain valve 5 and the water drain valve 10 to work.

The water in the water discharge valve 5 is supplied to the culture area through the water discharge valve 5, and when the water level in the culture area rises to the soaking water level set by the second liquid level meter, the second liquid level meter sends a signal to the controller, and the controller controls the water discharge valve to stop supplying water according to the signal.

The soaking time reaches the soaking time set by the controller, the controller controls the drain valve to work, the drain valve discharges water in the cultivation area outwards, and when the discharge water level reaches the dry dew water level set by the second liquid level meter, the second liquid level meter sends a signal to the controller, and the controller controls the drain valve to be closed according to the signal.

The dry exposure time reaches the dry exposure time set by the controller, the controller controls the water drain valve to work, water in the water quantity control area is supplied to the cultivation area through the water drain valve 5, and when the water level of the cultivation area rises to the soaking water level set by the second liquid level meter, the second liquid level meter sends a signal to the controller, and the controller controls the water drain valve to stop supplying water according to the signal. The cycle is repeated, thereby realizing the ocean tide-like phenomenon.

Example 3:

compared with the embodiment 1 or 2, the difference is that: in order to prevent the substrate from being discharged outwards following it, a mud flap 11 is added.

The mud guard 11 separates the cultivation area 6 into a drainage area and a cultivation cave bed area, and the drainage valve 10 is arranged in the drainage area; wherein, the distance between the mud guard and the drain valve 10 is 10-12 cm, and the height is 5-7 cm.

The distance between the mud guard and the drain valve 10 is 10, 11 or 12cm, and the height is 5, 6 or 7cm.

Example 4:

compared with example 1, the difference is that: in order to facilitate monitoring the water level and supplementing water in the water quantity control area, a first liquid level meter 2, a water inlet pipe 3 and a water inlet valve 4 are additionally arranged.

The water quantity control area 1 is provided with a first liquid level meter 2 and a water inlet pipe 3; the water inlet pipe 3 is provided with a water inlet valve 4; wherein, the first liquid level meter 2 and the water inlet valve 4 are electrically connected with the controller 12.

The working mode is as follows:

the first level gauge 2 is used for monitoring the water level in the water control area, and is set with a highest water level value and a lowest water level value.

When the water level of the water quantity control area is reduced to the lowest water level value set by the first liquid level meter, the first liquid level meter sends a signal to the controller, the controller controls the water inlet valve to work according to the signal, and the water inlet pipe supplements water to the water quantity control area;

when the water level of the water quantity control area rises to the set highest water level value of the first liquid level meter, the first liquid level meter sends a signal to the controller, the controller controls the water inlet valve to close according to the signal, and the water inlet pipe stops supplementing water to the water quantity control area.

The invention can conveniently monitor the water level of the water quantity control area, further can timely supplement water, is convenient for the water quantity control area to be kept within the set water level range, and can timely supply water when the water is needed in the cultivation area.

Example 5:

a method of breeding a sucking euglena using the sucking euglena land-based intensive culture pond of any one of embodiments 1 to 4, the method comprising the steps of;

(1) And (3) a culture pond: building a long box type cultivation pond with the width of 1.0-1.5 m and the depth of 40-50 cm, wherein the area of the pond is 1-100 square meters; the water quantity control area of the culture pond occupies 1/10-1/4 of the area of the culture pond, and is reserved to the required water level; a plurality of substrate cups are arranged on a culture cave bed in the culture area, and holes with the diameter of 10-12 mm are formed in the cup bottoms;

the water quantity control area occupies 1/10, 1/9, 1/8, 1/7, 1/6, 1/5 or 1/4 of the area of the culture pond;

the pore diameter of the pore formed at the bottom of the substrate cup is commonly 10, 11 or 12mm, etc., so that excretory substances of the sipunculus nudus can be conveniently exuded from the pore;

(2) Culture substrate: the dry dew is prepared from coarse sand, medium sand, fine powder sand and organic matters, and the humidity is kept at 25-45% per day; the culture medium is mainly prepared from the following raw materials in parts by weight: 15 parts of coarse sand with the grain size of 0.5-2 mm, 25 parts of middle sand with the grain size of 0.25-0.5 mm, 40 parts of fine sand with the grain size of 0.016-0.25 mm, 10 parts of fine sand with the grain size of 0.004-0.016 mm and 10 parts of organic matter;

(3) And (3) arranging a culture pond: adopting a land leveling arrangement method, wherein the distance between the culture ponds is 30-50 cm; the distance between the culture ponds is commonly 30 cm, 40 cm or 50 cm;

(4) Preparation before stocking: filling the culture substrate in the step (2) into each substrate cup of the culture cave bed, compacting, putting the culture cave bed into a culture area, supplying water to the culture area from a water quantity control area, and adjusting water inlet and drainage operation by setting the water level to be controlled by submerging the culture cave bed by more than 10 cm; the control water level is adjusted to enter and drain water to run: when water is fed, the cultivation area stops draining water outwards, the water quantity control area supplies water to the cultivation area, the water level in the cultivation area rises and submerges the cultivation cave bed, the cultivation cave bed is submerged to a set control water level, and the water quantity control area stops supplying water to the cultivation area; then, the water is discharged outwards from the cultivation area, and the water level is reduced until the cultivation cave bed is exposed;

the water level for controlling the water level adjustment water inlet and outlet operation can be set to be 10, 12, 15, 18, 20, 25, 30 or 35 cm;

(5) And (5) stocking seedlings: selecting seedlings which are light in body color, dark in gray, neat in specification, free of body surface injury, strong in vitality and free of kissing and stretching, stocking the seedlings with the stocking specification of 800 tails/kg and stocking density of 1 tail/cup, draining water, digging out a substrate, placing the seedlings in the substrate cup, and covering the substrate; the method comprises the following steps: draining pond water in a culture area, digging 1/4-1/3 of matrixes in a matrix cup, then placing the matrixes in a matrix cup of the delicious sipunculus nudus Miao Yu according to 1 tail/cup, covering the digging matrixes back and covering the matrixes with the delicious sipunculus nudus seedlings, observing for 2-3 days, and after the delicious sipunculus nudus seedlings form a cave in the matrix cup, feeding water to the culture area by a water quantity control area to a set water level; during the period, the delicious euglena seedlings need to be replaced when the substrate cup is drilled;

(6) And (3) cultivation management:

(1) and (3) water inlet and drainage: according to seasons, weather and growth conditions of the delicious euglena, simulating ocean tide phenomenon, feeding and draining water 1 time a day, wherein the culture water level in spring and winter is more than 10 cm higher than that of a culture cave bed, the culture water level in summer, autumn or rainy days is more than 20cm higher than that of the culture cave bed, the immersion time of the culture cave bed is 12 hours, the dry dew time is 12 hours, and the soil humidity is kept to be 25-45% per day;

the water level for spring and winter cultivation is 10, 12, 14, 15, 18 or 20cm higher than that of the cultivation cave bed;

the culture water level in summer, autumn or rainy days is 20, 25, 28, 30 or 35cm higher than that of the culture cave bed;

the humidity of the soil is usually 25, 30, 35, 40 or 45% per day, etc. when the dry dew is kept;

(2) feeding: algae, fish meal or bean pulp powder is fed for 1 time every day, and the feeding method comprises the following steps: before water enters the cultivation area, algae, fish meal or bean pulp powder is put in a water quantity control area, and when water enters the cultivation area from the water quantity control area, the algae, the fish meal or the bean pulp powder enters the cultivation area through water flowing;

(3) and (3) patrol: checking for weather and facility operation conditions, checking for ingestion, growth and disease conditions of the delicious sipunculus nudus, making shading and storm prevention measures, controlling the salinity of the water for cultivation to be 7-30 per mill, the PH value to be 7.5-8.5, the dissolved oxygen to be more than 5 per liter, and keeping the humidity of the matrix dry dew to be 25-45 per day; measuring the body length and the body weight for 1 time every 30 days to adjust the feeding scheme;

the salinity of the water for cultivation is controlled to be 7 per mill, 10 per mill, 15 per mill, 18 per mill, 19 per mill, 20 per mill, 25 per mill, 28 per mill, 30 per mill or the like;

the pH value can be 7.5, 8 or 8.5;

the substrate dry dew retention humidity is typically 25%, 30%, 32%, 35%, 38%, 40%, 42%, 45% or the like;

(4) harvesting: culturing the delicious euglena with the stocking specification of 800 tails/kg for 12 months and 6 days in 2020, harvesting the delicious euglena to 2021 for 11 months and 30 days, culturing for 359 days, namely draining pond water, taking out a breeding hole bed from a culturing area, turning upside down, pouring out a matrix, digging out and dispersing the matrix, and searching for all the harvested delicious euglena; after harvesting, the substrate was exposed to the sun for 15 days and then returned to the substrate cup.

The following examples differ from example 5 in that: the different amounts of each component in the culture matrix, the stocking specification, the immersion time of the culture cave bed, the dry exposure time of the culture cave bed and the different culture times are shown in the following table.

TABLE 1 different amounts of raw materials of culture substrate, stocking specifications, submerged culture cave bed and dry dew time in the invention

Name of the name

Coarse sand

Middle sand

Fine sand

Fine silt

Organic matter

Stocking specification (Tail/kg)

Infusion of cultivating cave bedNo (h)

Cultivation cave bed dry dew (h)

Example 6

15 parts of

25 parts of

40 parts of

10 parts of

7.5 parts

800

12

12

Example 7

18 parts of

22 parts of

45 parts of

5 parts of

10 parts of

850

13

11

Example 8

20 parts of

20 parts of

35 parts of

15 parts of

10 parts of

800

14

10

Example 9

20 parts of

15 parts of

35 parts of

20 parts of

10 parts of

800

15

9

Example 10

15 parts of

15 parts of

50 parts of

15 parts of

5 parts of

900

16

8

Example 11

22 parts of

18 parts of

30 parts of

20 parts of

10 parts of

800

12

12

Note that: the grain size of coarse sand is 0.5-2 mm, the grain size of medium sand is 0.25-0.5 mm, the grain size of fine sand is 0.016-0.25 mm, the grain size of fine powder sand is 0.004-0.016 mm,

example 6: culturing the delicious euglena with the stocking specification of 800 tails/kg for 18 days in 12 months in 2020, harvesting from 2021 to 11 months for 30 days, culturing for 347 days, draining pond water, taking out the culture cave bed from the culture area, turning upside down, pouring out the matrix, digging out and dispersing the matrix, and searching for all the harvested delicious euglena; after harvesting, the substrate was exposed to the sun for 15 days and then returned to the substrate cup.

Example 7: culturing the delicious euglena with the stocking specification of 850 ends/kg for 18 days in 12 months in 2020, harvesting from 2021 to 11 months for 30 days, culturing for 347 days, draining pond water, taking out the culture cave bed from the culture area, turning upside down, pouring out the matrix, digging out and dispersing the matrix, and searching for all the harvested delicious euglena; after harvesting, the substrate was exposed to the sun for 30 days and then returned to the substrate cup.

Example 8: culturing the delicious euglena with the stocking specification of 800 tails/kg for 18 days in 12 months in 2020, harvesting from 2021 to 11 months for 30 days, culturing for 347 days, draining pond water, taking out the culture cave bed from the culture area, turning upside down, pouring out the matrix, digging out and dispersing the matrix, and searching for all the harvested delicious euglena; after harvesting, the substrate was exposed to the sun for 25 days and then returned to the substrate cup.

Example 9: culturing the delicious euglena with the stocking specification of 800 tails/kg for 18 days in 2021 and harvesting from 2021 to 11 months for 30 days, culturing for 316 days, namely draining pond water, taking out a culturing cave bed from a culturing area, turning upside down, pouring out a matrix, digging out and dispersing the matrix, and searching for all the harvested delicious euglena; after harvesting, the substrate was exposed to the sun for 18 days and then returned to the substrate cup.

Example 10: culturing the delicious euglena with the stocking specification of 800 tails/kg for 18 days in 2021 and harvesting from 2021 to 11 months for 30 days, culturing for 316 days, namely draining pond water, taking out a culturing cave bed from a culturing area, turning upside down, pouring out a matrix, digging out and dispersing the matrix, and searching for all the harvested delicious euglena; after harvesting, the substrate was exposed to the sun for 20 days and then returned to the substrate cup.

Example 11: culturing the delicious euglena with the stocking specification of 800 tails/kg for 18 days in 2021 and harvesting from 2021 to 11 months for 30 days, culturing for 316 days, namely draining pond water, taking out a culturing cave bed from a culturing area, turning upside down, pouring out a matrix, digging out and dispersing the matrix, and searching for all the harvested delicious euglena; after harvesting, the substrate was exposed to the sun for 15 days and then returned to the substrate cup.

Example 12: compared with example 5, the difference is that: the culture ponds are arranged in a goods shelf mode, and the superposition distance between the culture ponds is 60-80 cm; when the shelf type arrangement is adopted, the superposition distance between the culture ponds is 60, 70 or 80 cm.

The organic matters of the invention comprise fermented peanut bran, rice bran, bean hull and the like. The weight ratio of the fermented peanut bran, rice bran and bean hull is 1-3: 2 to 4:1 to 4; the weight ratio of the fermented peanut bran, rice bran and bean hull is generally 1:2: 1. 2:3:2.5 or 3: 4: 4, etc.

Comparative examples:

comparative example 1: the comparison example 1 is different from the invention in that the cultivation field of the comparison example 1 is a salty acid field, furrows are dug in the field by 30-50 cm, ridges with the width of 1-2 m are built, the delicious sipunculus nudus seedlings are put on the ridge surfaces for cultivation, the stocking density is 400-500 feet per square meter, the sea tide expansion and damping rules are simulated by dry exposure and immersion, water pumping regulation and control are carried out, and the stocking specification and daily management measures are the same as those of the examples 5-11. The method of the comparative example is used for cultivating the delicious euglena for 359 days, and the average specification of 309 tails/kg is harvested, and the survival rate is 62.3%.

Comparative example 2: the comparison example 2 is different from the invention in that the cultivation field of the comparison example 2 is an old shrimp pond, furrows are dug at the bottom of the pond for 30-50 cm, ridges with the width of 1-2 m are built, the delicious sipunculus nudus fries are put on the ridge surface for cultivation, the stocking density is 400-500 feet per square meter, the dry dew and the immersion utilize ocean tide to expand and damp, and the stocking specification and daily management measures are the same as those of the examples 5-11 through the regulation and control of the water gate of the shrimp pond. The method of the comparative example is used for cultivating the delicious euglena for 359 days, and the average specification of 324 tails/kg is harvested, and the survival rate is 57.8%.

Comparative example 3: the difference between the comparative example 3 and the invention is that the cultivation field of the comparative example 3 is a beach with natural and delicious sipunculus nudus growth, furrows are dug for 30-50 cm, ridges with the width of 1-2 m are built on the beach, and the delicious sipunculus nudus seedlings are put on the ridge surface for cultivation, the stocking density is 400-500 per square meter, the dry exposure and the immersion are automatically regulated by ocean tides, and the stocking specification and daily management measures are the same as those of the examples 5-11. The method of the comparative example is used for culturing the delicious euglena for 347 days, and the average specification of 442 tails/kg is harvested, and the survival rate is 46.5%.

The cultivation time, survival rate and average harvest specification of the cultivation of the pungent sipunculus nudus in the present invention and comparative examples 1, 2 and 3 are shown in table 2.

TABLE 2 cultivation time, survival rate and average harvesting Specification for the cultivation of the delicious Phascophyllum inophyllum

Project	Cultivation time (d)	Survival rate (%)	Average specification (Tail/Kg)	Average body weight (g/tail)
					Example 5	359	82.8	201	5.0
Example 6	347	85.9	231	4.3
					Example 7	347	87.2	216	4.6
Example 8	347	85.3	248	4.0
					Example 9	316	84.1	227	4.4
Example 10	316	88.6	256	3.9
					Example 11	316	84.7	236	4.2
Comparative example 1 (salty sour field)	359	62.3	309	3.2
					Comparative example 2 (old shrimp pond)	359	57.8	324	3.1
Comparative example 3 (beach)	347	46.5	442	2.3

As can be seen from Table 2, the cultivation method of the invention provides the cultivation method of the delicious sipunculus nudus, which is used for cultivating the delicious sipunculus nudus for 316-359 days (about 10-12 months), and the harvesting specification is 201-256 (about 200-260 tails/kg), and the cultivation survival rate is more than 82.8%.

Compared with the data of the comparative example 1, the survival rate of the cultured delicious euglena is improved by 20.5%, and the average weight is increased by 1.8g. The method is superior to the culture method used in the comparative example, the facility can prevent the organisms such as fish, shrimp, crab, bird and the like from damaging the delicious sipunculus similis from entering the culture pond, and can ensure that the delicious sipunculus similis grows well, and the culture survival rate and the growth speed are higher than those of the comparative example.

From the data of comparative example 2, comparative example 2 showed only 57.8% of the survival rate of the tasty euglena, and the average body weight was also lower than that of the present invention. The method shows that the culture environment of the sipunculus nudus cultured in the pond is not well controlled, and is influenced by weather and harmful organisms in the pond, so that the growth and development of the sipunculus nudus are influenced, and the survival rate and the individual specification are reduced.

From the data of comparative example 3, the survival rate and average body weight of the tasty euglena of comparative example 3 were much lower than that of the present invention. The method shows that the method is greatly affected by weather, waves, harmful organisms and the like when the method is used for cultivating the delicious sipunculus nudus in uncontrollable beach, and is easy to cause low cultivation survival rate of the delicious sipunculus nudus.

The method for cultivating the delicious euglena has the advantages of high survival rate, regular specification, high growth speed, convenient cultivation and management and low cost, can realize the large-scale cultivation of the delicious euglena, and has good application prospect.

It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being within the scope of the invention, obvious variations or modifications may be made thereto.

Claims (6)

1. A method for cultivating delicious euglena is characterized in that: the method comprises the following steps;

(1) And (3) a culture pond: building a long box type cultivation pond with the width of 1.0-1.5 m and the depth of 40-50 cm, wherein the area of the pond is 1-100 square meters;

the culture pond is divided into a water quantity control area (1) and a culture area (6); a culture cave bed (7) is movably arranged in the culture area (6); the culture cave bed (7) comprises a culture rack (71) and substrate cups (72), wherein the culture rack (71) is provided with a plurality of substrate cups (72); wherein the water quantity control area (1) supplies water quantitatively to the cultivation area (6); the cultivation area (6) is provided with drainage water;

(2) Culture substrate: the dry dew is prepared from coarse sand, medium sand, fine powder sand and organic matters, and the humidity is kept at 25-45% per day;

(3) And (3) arranging a culture pond: adopting a land leveling arrangement method, wherein the distance between the culture ponds is 30-50 cm; or, adopting shelf type arrangement, wherein the superposition distance between the culture ponds is 60-80 cm;

(4) Preparation before stocking: filling the culture substrate in the step (2) into each substrate cup of the culture cave bed, compacting, putting the culture cave bed into a culture area, supplying water to the culture area from a water quantity control area, and adjusting water inlet and drainage operation by setting the water level to be controlled by submerging the culture cave bed by more than 10 cm;

(5) And (5) stocking seedlings: selecting seedlings which are light in body color, dark in gray, neat in specification, free of body surface injury, strong in vitality and free of kissing and stretching, stocking the seedlings with stocking specification of 800-900 tails/kg and stocking density of 1 tail/cup, and stocking the seedlings by adopting a method of draining water, digging out a substrate, stocking the seedlings in a substrate cup and covering the substrate;

(6) And (3) cultivation management: the sea tide phenomenon is imitated, the water is fed and discharged for 1 time every day, the immersion time of the culture cave bed is 12-16 hours, and the dry exposure time is 8-12 hours; feeding algae, fish meal or bean pulp powder for 1 time every day; culturing to 200-260 tails/kg, taking out the breeding hole bed from the culture area, turning upside down, pouring out the matrix, and harvesting the delicious euglena;

the water quantity control area of the culture pond in the step (1) occupies 1/10-1/4 of the area of the culture pond, and is reserved to the required water level; a plurality of substrate cups are arranged on a culture cave bed in the culture area, and holes with the diameter of 10-12 mm are formed in the cup bottoms;

the culture medium in the step (2) is mainly prepared from the following raw materials in parts by weight: 15-20 parts of coarse sand with the grain size of 0.5-2 mm, 15-25 parts of medium sand with the grain size of 0.25-0.5 mm, 30-50 parts of fine sand with the grain size of 0.016-0.25 mm, 5-20 parts of fine powder sand with the grain size of 0.004-0.016 mm and 5-10 parts of organic matters;

the method for draining water, digging up the substrate, placing seedlings in a substrate cup and covering the substrate in the step (5): draining pond water in a culture area, digging 1/4-1/3 of matrixes in a matrix cup, then placing the matrixes in a matrix cup of the delicious sipunculus nudus Miao Yu according to 1 tail/cup, covering the digging matrixes back and covering the matrixes with the delicious sipunculus nudus seedlings, observing for 2-3 days, and after the delicious sipunculus nudus seedlings form a cave in the matrix cup, feeding water to the culture area by a water quantity control area to a set water level; during this period, the artemia salina seedlings were replaced if they were to drill out the substrate cup.

2. The method for breeding the delicious euglena according to claim 1, wherein: in the step (4), the control water level is used for debugging water inlet and drainage operation: when water is fed, the cultivation area stops draining water outwards, the water quantity control area supplies water to the cultivation area, the water level in the cultivation area rises and submerges the cultivation cave bed, the cultivation cave bed is submerged to a set control water level, and the water quantity control area stops supplying water to the cultivation area; then, the cultivation area drains outwards, and the water level drops to expose the cultivation cave bed.

3. The method for breeding the delicious euglena according to claim 1, wherein: the managing of the cultivation in the step (6) comprises the following steps:

(1) and (3) water inlet and drainage: according to seasons, weather and growth conditions of the delicious euglena, simulating ocean tide phenomenon, feeding and draining water 1 time a day, wherein the culture water level in spring and winter is 10 cm above the culture cave bed, the culture water level in summer, autumn or rainy days is 20cm above the culture cave bed, the immersion time of the culture cave bed is 12-16 hours, the dry dew time is 8-12 hours, and the soil humidity is kept 25-45% per day;

(2) feeding: algae, fish meal or bean pulp powder is fed for 1 time every day, and the feeding method comprises the following steps: before water enters the cultivation area, algae, fish meal or bean pulp powder is put in a water quantity control area, and when water enters the cultivation area from the water quantity control area, the algae, the fish meal or the bean pulp powder enters the cultivation area through water flowing;

(3) and (3) patrol: checking for weather and facility operation conditions, checking for ingestion, growth and disease conditions of the delicious sipunculus nudus, making shading and storm prevention measures, controlling the salinity of the water for cultivation to be 7-30 per mill, the PH value to be 7.5-8.5, the dissolved oxygen to be more than 5 per liter, and keeping the humidity of the matrix dry dew to be 25-45 per day; measuring body length and body weight 1 time every 30 days to adjust bait feeding scheme;

(4) harvesting: culturing the delicious sipunculus nudus to 200-260 tails/kg, draining pool water, taking out the culture cave bed from the culture area, turning upside down, pouring out the matrix, digging out and dispersing the matrix, and searching for all the collected delicious sipunculus nudus; after harvesting, the substrate is exposed for more than 15 days and then returned to the substrate cup.

4. The method for breeding the delicious euglena according to claim 1, wherein: the device also comprises a second liquid level meter (8), a water drain valve (5), a drain valve (10) and a controller (12);

the water quantity control area (1) is connected with the cultivation area (6) through a water drain valve (5);

the culture area (6) is provided with a second liquid level meter (8) and a drain valve (10);

the controller (12) is arranged in the water quantity control area (1) or the cultivation area (6);

the second liquid level meter (8), the water drain valve (5) and the water drain valve (10) are electrically connected with the controller (12).

5. The method for cultivating a delicious euglena according to claim 4, wherein: also comprises a mud guard (11);

the mud guard (11) separates the cultivation area (6) into a drainage area and a cultivation cave bed area, and the drainage valve (10) is arranged in the drainage area; wherein the distance between the mud guard and the drain valve (10) is 10-12 cm, and the height is 5-7 cm.

6. The method for cultivating a delicious euglena according to claim 4 or 5, wherein: the device also comprises a first liquid level meter (2), a water inlet pipe (3) and a water inlet valve (4);

the water quantity control area (1) is provided with a first liquid level meter (2) and a water inlet pipe (3);

the water inlet pipe (3) is provided with a water inlet valve (4);

wherein, first level gauge (2), water intaking valve (4) all are connected with controller (12) electricity.