CN114230006A - Natural enrichment method for anaerobic ammonium oxidation bacteria - Google Patents
Natural enrichment method for anaerobic ammonium oxidation bacteria Download PDFInfo
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- 241000894006 Bacteria Species 0.000 title claims abstract description 48
- 230000003647 oxidation Effects 0.000 title claims abstract description 41
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 37
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 title claims abstract description 31
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 51
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 44
- 229910052717 sulfur Inorganic materials 0.000 claims abstract description 43
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- 230000001651 autotrophic effect Effects 0.000 claims abstract description 27
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- 239000010802 sludge Substances 0.000 claims abstract description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 13
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 8
- 238000012163 sequencing technique Methods 0.000 claims abstract description 3
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- 238000006243 chemical reaction Methods 0.000 claims description 33
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- 230000035484 reaction time Effects 0.000 claims description 16
- 229910021529 ammonia Inorganic materials 0.000 claims description 13
- 239000000945 filler Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 5
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- 230000008569 process Effects 0.000 description 8
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
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- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
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- 238000005728 strengthening Methods 0.000 description 3
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/282—Anaerobic digestion processes using anaerobic sequencing batch reactors
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2853—Anaerobic digestion processes using anaerobic membrane bioreactors
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/286—Anaerobic digestion processes including two or more steps
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2866—Particular arrangements for anaerobic reactors
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C02F2209/15—N03-N
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
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- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
A natural enrichment method of anaerobic ammonium oxidation bacteria, belonging to the technical field of biological sewage treatment. Under the anaerobic condition, the invention selects a biofilm sequencing batch reactor, and the temperature is 19-25 ℃. Firstly, the membrane is formed under the conditions that the carbon-nitrogen ratio is 3/1 and the nitrate nitrogen ratio is 3/1, so that the half-course denitrification of the nitrate nitrogen is realized, and the removal of the ammonia nitrogen is not found at the stage. Then inoculating sulfur autotrophic denitrification sludge, replacing an organic carbon source with a sulfur simple substance, starting sulfur autotrophic denitrification, and guiding the reaction type without regulating the ratio of the sulfur simple substance to nitrate nitrogen. The reduction of nitrate nitrogen and the removal of ammonia nitrogen are realized at the stage, and the natural enrichment of anaerobic ammonium oxidation bacteria occurs. And then, in order to further enrich and strengthen the anaerobic ammonium oxidation bacteria, the proportion of nitrate nitrogen to ammonia nitrogen is gradually increased to 3/2 and 3/3, and the synchronous generation of sulfur autotrophic denitrification and anaerobic ammonium oxidation and higher total nitrogen removal are realized.
Description
Technical Field
The invention relates to the field of biological sewage treatment, in particular to an anaerobic ammonium oxidation bacterium enrichment method.
Background
With the acceleration of urbanization, the demands and challenges of sewage treatment are increasing. The biological denitrification is a main method for the current sewage denitrification, and has the advantages of economy, high efficiency and small secondary pollution. Conventional biological denitrification generally involves two steps, namely nitrification and denitrification. In the denitrification process, the denitrifying bacteria firstly reduce nitrate nitrogen into nitrite nitrogen, and then further reduce the nitrite nitrogen into nitrogen, thereby completing the removal of nitrogen. However, the method has high energy consumption, and usually needs additional organic carbon sources, so the economic cost is high. Therefore, there is a need to search for more efficient and energy-efficient biological denitrification processes.
Through continuous exploration, the anaerobic ammonia oxidation technology which is an efficient and energy-saving technology is emphasized, and ammonia nitrogen and nitrite nitrogen are converted into nitrogen in one step under an anoxic environment, so that the nitrogen is removed. The anaerobic ammonia oxidation shows higher denitrification performance, does not need an external carbon source, has lower sludge yield and reduces the operation cost, and is a high-efficiency and environment-friendly biological denitrification technology. However, anammox bacteria are sensitive to the environment and grow at a slower rate. Particularly, the growth of the anaerobic ammonium oxidation bacteria is difficult to be well supported under the conditions of low ammonia nitrogen concentration and low temperature. Therefore, the problem that how to realize the self-enrichment of the anaerobic ammonium oxidation bacteria under the normal temperature condition is urgently needed to be solved aiming at the low ammonia nitrogen wastewater.
Disclosure of Invention
The invention aims to provide a strategy for self-enriching anaerobic ammonium oxidation bacteria under the condition of not inoculating anaerobic ammonium oxidation sludge in a starting process of sulfur autotrophic denitrification with a sulfur simple substance as a substrate under the normal temperature condition. In order to achieve the purpose, the technical scheme of the invention is as follows:
the method comprises the following steps: constructing a reaction device, wherein the reaction device can realize intermittent water inlet and outlet and provide an anoxic environment, the reactor is a Sequencing Batch Reactor (SBR), the anoxic environment providing device is a stirring paddle, the filler is polyurethane sponge filler, the filling ratio is 20-50%, and the drainage ratio is 40-60%;
step two: feeding water into the first stage of the reaction device, adding a carbon source, wherein the mass concentration ratio of carbon to nitrogen is 2.5/1-3.5/1, so that the filler is subjected to biofilm formation, and performing heterotrophic half-range denitrification, wherein the feed water in the first stage comprises nitrate nitrogen and ammonia nitrogen; the reaction time of each period of the first stage is 30-50min, and 200-280 periods are carried out;
step three: feeding water into the second stage of the reaction device, adding a sulfur simple substance with the concentration of 0.05-0.2g/L into the water at each time, inoculating sulfur autotrophic denitrification sludge in the proportion of 1/5-1/10, and replacing heterotrophic denitrification with sulfur autotrophic denitrification to realize natural enrichment of anaerobic ammonia oxidation, wherein the water fed into the second stage comprises nitrate nitrogen and ammonia nitrogen, the mass concentration ratio of the nitrate nitrogen to the ammonia nitrogen is 2/1-4/1, the reaction time of each period is 5-6h, and the period is 80-120;
optionally, the method may further include:
step four: feeding water into the reaction device at the third stage, and adding a sulfur simple substance with the concentration of 0.05-0.2g/L to strengthen the enrichment of anaerobic ammonium oxidation bacteria, wherein the proportion of ammonia nitrogen in the water fed into the reaction device at the third stage is greater than that in the water fed into the first stage and the water fed into the reaction device at the second stage. The reaction time of each period is 5-6h, and the reaction is carried out for 40-70 periods;
step five: and (3) feeding water into the fourth stage of the reaction device, adding a sulfur simple substance with the concentration of 0.05-0.2g/L into the water in each time to strengthen the enrichment of the anammox bacteria, wherein the proportion of ammonia nitrogen in the water fed into the fourth stage is greater than that in the water fed into the third stage, and the reaction time in each period is 5-6h, and carrying out 40-70 periods.
In the operation process of the method, the reaction temperature is 19-25 ℃, and the pH is 7.0-8.0.
The method uses the sulfur simple substance to replace an organic carbon source, and successfully and naturally enriches the anaerobic ammonium oxidation bacteria on the premise of not inoculating the anaerobic ammonium oxidation sludge in the process of starting sulfur autotrophic denitrification at normal temperature aiming at the low-ammonia nitrogen wastewater. The invention has the beneficial effects that:
(1) the invention is suitable for biological denitrification of sewage, overcomes the problem of environmental sensitivity of anammox bacteria, and provides a new strategy for enriching the anammox bacteria at normal temperature;
(2) according to the invention, on the premise of not inoculating anaerobic ammonium oxidation sludge, anaerobic ammonium oxidation bacteria are naturally enriched in the biomembrane SBR.
(3) The discovery realizes the coupling of the sulfur autotrophic denitrification and the anaerobic ammonia oxidation, and strengthens the simultaneous removal of ammonia nitrogen and nitrate nitrogen in the sewage. The strategy is easy to popularize in practice.
(4) Inoculating sulfur autotrophic denitrification sludge, replacing an organic carbon source with a sulfur simple substance, starting sulfur autotrophic denitrification, and guiding the reaction type without regulating the ratio of the sulfur simple substance to nitrate nitrogen. The reduction of nitrate nitrogen and the removal of ammonia nitrogen are realized at the stage, and the natural enrichment of anaerobic ammonium oxidation bacteria occurs. And then, in order to further enrich and strengthen the anaerobic ammonium oxidation bacteria, the proportion of nitrate nitrogen to ammonia nitrogen is gradually increased to 3/2 and 3/3, and the synchronous generation of sulfur autotrophic denitrification and anaerobic ammonium oxidation and higher total nitrogen removal are realized. The invention is suitable for biological denitrification of sewage, overcomes the problem of environmental sensitivity of anammox bacteria, and provides a new strategy for enriching the anammox bacteria at normal temperature.
Detailed Description
The technical solution of the present invention will be described with reference to the following examples. It is to be understood that the described embodiments are merely a few, and not all, of the present invention. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present application shall fall within the scope of the present application.
The sulfur autotrophic denitrification uses elemental sulfur as an electron donor, namely an autotrophic biological denitrification reaction, and has economic feasibility and low sludge yield. In the process of sulfur autotrophic denitrification, substrate nitrite nitrogen can be provided for anaerobic ammonium oxidation bacteria.
In addition, after the sewage is subjected to secondary treatment, nitrate nitrogen is generated, so that the effluent cannot reach the standard. At this stage, there is not enough organic carbon available to allow the microorganisms to reduce the nitrate nitrogen. The sulfur autotrophic denitrification can overcome the problems and deeply denitrify the sewage. Therefore, if anaerobic ammonia oxidation can be carried out simultaneously in the process of normal-temperature sulfur autotrophic denitrification, the denitrification capability of the sewage can be greatly enhanced, and the method is an attractive strategy for deep denitrification of the sewage.
Based on the above, the invention provides an anaerobic ammonium oxidation bacteria enrichment method based on sulfur autotrophic denitrification. The method may comprise the steps of:
step one, constructing a reaction device. The reaction device comprises a reactor, an anoxic environment providing device and a filler, wherein the reactor can be an SBR reactor, the anoxic environment providing device can be a stirring paddle, and the filler can be a polyurethane sponge filler. The reaction device takes the conditions of realizing intermittent water inlet and outlet and providing an anoxic environment as the standard, and the invention does not limit the type of the reaction device and the specific parameters thereof.
Step two, filling and film forming, and heterotrophic half-range denitrification: and (3) feeding water into the first stage of the reaction device, adding a carbon source according to a carbon-nitrogen ratio of 3/1, wherein the water discharge ratio is 40-60%, so that the filler is subjected to film formation, and performing heterotrophic half-process denitrification. The first-stage inlet water comprises nitrate nitrogen and ammonia nitrogen, and the mass concentration ratio of the nitrate nitrogen to the ammonia nitrogen is 2/1-4/1. The carbon source may be sodium acetate, methanol, glucose, etc. The reaction time of each period of the first stage is 30-50min, and 200-280 periods are carried out.
Step three, sulfur autotrophic denitrification: feeding water into the second stage of the reaction device, and adding a sulfur simple substance with the concentration of 0.05-0.2 g/L. Inoculating sulfur autotrophic denitrification sludge with the inoculation ratio of 1/5-1/10, and replacing heterotrophic denitrification by autotrophic denitrification to realize natural enrichment of anaerobic ammonia oxidation. The second stage inlet water comprises nitrate nitrogen and ammonia nitrogen, the mass concentration ratio of the nitrate nitrogen to the ammonia nitrogen is 2/1-4/1, and the drainage ratio is 40-60%. The reaction time of each period is 5-6h, and the reaction is carried out for 80-120 periods.
Optionally, to further enrich for anammox bacteria, the method may further comprise:
step four, enriching and strengthening anaerobic ammonium oxidation bacteria: and feeding water into the third stage of the reaction device, and adding a sulfur simple substance with the concentration of 0.05-0.2g/L to enrich and strengthen the anaerobic ammonium oxidation bacteria. The third stage feed water comprises nitrate nitrogen and ammonia nitrogen, the proportion of the ammonia nitrogen of the third stage feed water is larger than that of the ammonia nitrogen of the first stage feed water and the second stage feed water, and the drainage ratio is 40-60%. The reaction time of each period is 5-6h, and 40-70 periods are carried out.
Fifthly, enriching the anaerobic ammonium oxidation bacteria for secondary reinforcement: and (3) feeding water into the fourth stage of the reaction device, and adding a sulfur simple substance with the concentration of 0.05-0.2g/L to enrich and strengthen the anaerobic ammonium oxidation bacteria again. The fourth stage inlet water comprises nitrate nitrogen and ammonia nitrogen, the proportion of the ammonia nitrogen of the fourth stage inlet water is larger than that of the ammonia nitrogen of the third stage inlet water, and the drainage ratio is 40-60%. The reaction time of each period is 5-6h, and 40-70 periods are carried out.
Example 1
In this example, an SBR reactor was used as a reaction apparatus, the effective volume was 3L, and the water discharge ratio was 50%. The activated sludge is inoculated in an anoxic zone of a certain municipal sewage treatment plant in Beijing, and the sludge concentration is 4500-5500 mg/L. The reaction temperature is 19-25 deg.C (normal temperature), and pH is 7.0-8.0. Stirring by using a stirring paddle in the whole reaction process, and maintaining an anoxic environment. Polyurethane sponge filler is added into the reactor, and the filling ratio is 30%.
The first stage is as follows: and (3) film formation by filling materials and a heterotrophic half-way denitrification stage.
The quality of inlet water is as follows: nitrate nitrogen: 40.7 + -4.13 mg/L (from NaNO)3Provided), ammonia nitrogen 14.1 +/-4.6 mg/L (by NH)4Cl), COD: 120.5. + -. 21.2mg/L (provided by sodium acetate). This stage maintained a C/N of 3/1, allowing half-way denitrification in the system. SBR was run for 8 cycles per day with a reaction time of 40min per cycle for a total of 200 cycles.
The ammonia nitrogen in the effluent is 13.7 +/-2.3 mg/L, and the ammonia nitrogen removal rate is lower than 5 percent (negligible). This stage does not substantially counteract the removal of ammonia nitrogen under anoxic conditions. In addition, the concentration of effluent nitrate nitrogen is 5.9 plus or minus 2.9mg/L, nitrite nitrogen is 33.8 plus or minus 4.2mg/L, the average nitrite accumulation rate is 73.0 percent, and the effluent nitrate nitrogen has half-way denitrification performance. The biomass of each filler was approximately 43.4mg, and the film formation was considered successful.
And a second stage: a sulfur autotrophic denitrification stage.
The nitrate nitrogen/ammonia nitrogen at this stage is 3/1. The quality of inlet water is as follows: nitrate nitrogen: 40.7 + -4.13 mg/L (from NaNO)3Provided), ammonia nitrogen 14.1 +/-4.6 mg/L (by NH)4Cl supply), adding 0.16g/L (namely 0.5g) of sulfur elementary substance per period, and adding NaHCO3The alkalinity is provided. Inoculating sulfur autotrophic denitrification sludge which does not have anaerobic ammonia oxidation capacity, wherein the inoculation ratio is 1: 10. SBR was run for 4 cycles per day with a reaction time of 5h 30min per cycle for a total of 100 cycles.
The experimental results are as follows: in 44 periods after inoculation, the average reduction rate of nitrate nitrogen is 16.4%, the average removal rate of ammonia nitrogen is 5.8%, and the average accumulation rate of nitrite is 64.0%. In the 48 th period after inoculation, the reduction of the nitrate nitrogen and the removal of the ammonia nitrogen occur simultaneously, the reduction of the nitrate nitrogen reaches 62.6 percent, and the removal rate of the ammonia nitrogen is improved to 33.4 percent. In 50 periods after the nitrate nitrogen and the ammonia nitrogen are removed, the average reduction rate of the nitrate nitrogen is 54.1 percent, and the average removal rate of the ammonia nitrogen is 67.0 percent. The natural enrichment of the anammox bacteria is realized in the stage.
The method is effective and verified: and (3) carrying out an anaerobic ammonia oxidation activity test, only adding ammonia nitrogen and nitrite nitrogen (the proportion is 1/1.32) into inlet water, and calculating the contribution rate of anaerobic ammonia oxidation bacteria to denitrification to be 57.1%. At this stage, it is considered that the anaerobic ammonium oxidation bacteria are naturally enriched in the starting process of the sulfur autotrophic denitrification under the normal temperature condition that the anaerobic ammonium oxidation bacteria are not inoculated.
Example 2
In order to further enrich anammox bacteria, this embodiment further includes, based on the operation of the first embodiment:
and a third stage: and (5) enriching and strengthening anaerobic ammonium oxidation bacteria.
The nitrate nitrogen/ammonia nitrogen at this stage is 3/2. The quality of inlet water is as follows: nitrate nitrogen: 40.7 + -4.13 mg/L (from NaNO)3Provided), ammonia nitrogen 29.7 +/-3.3 mg/L (from NH)4Cl supply), adding 0.16g/L (namely 0.5g) of sulfur simple substance per period, and adding NaHCO3The alkalinity is provided. SBR was run for 4 cycles per day with a reaction time of 5h 30min per cycle for a total of 56 cycles.
The experimental results are as follows: in the stage, in 32 periods, the reduction rate of nitrate nitrogen is increased to 87.6%, and the removal rate of ammonia nitrogen is increased to 87.47%. Then, the reduction rate of the nitrate nitrogen is maintained at about 85 percent, and the removal rate of the ammonia nitrogen exceeds 95 percent.
The method is effective and verified: and (3) carrying out an anaerobic ammonia oxidation activity test, wherein only ammonia nitrogen and nitrite nitrogen (the proportion is 1/1.32) are added into inlet water, and the contribution rate of anaerobic ammonia oxidizing bacteria to denitrification is 73.3% according to calculation. It is believed that anammox bacteria are enriched.
Example 3
In order to further enrich anammox bacteria, this embodiment further includes, based on the operation of the second embodiment:
a fourth stage: and (3) enriching anaerobic ammonium oxidation bacteria for a second strengthening stage.
The nitrate nitrogen/ammonia nitrogen at this stage is 3/3. The quality of inlet water is as follows: nitrate nitrogen: 43.6 + -3.3 mg/L (from NaNO)3Provided), ammonia nitrogen 42.1 +/-2.1 mg/L (from NH)4Cl supply), adding 0.16g/L (namely 0.5g) of sulfur simple substance per period, and adding NaHCO3The alkalinity is provided. SBR was run for 4 cycles per day with a reaction time of 5h 30min per cycle for a total of 56 cycles.
The experimental results are as follows: in the stage, in a 28-period, the reduction rate of nitrate nitrogen is increased to 88.5%, and the removal rate of ammonia nitrogen is increased to 83.2%. Then, the reduction rate of the nitrate nitrogen is maintained at about 86.2%, and the removal rate of the ammonia nitrogen is maintained at 88.1%.
The method is effective and verified: and (3) carrying out an anaerobic ammonia oxidation activity test, only adding ammonia nitrogen and nitrite nitrogen (the proportion is 1/1.32) into inlet water, and calculating the contribution rate of anaerobic ammonia oxidation bacteria to denitrification to be 82.0%. It is considered that the anammox bacteria are enhanced.
The foregoing is a detailed description of the invention that will enable those skilled in the art to better understand and practice the invention, and it is not to be limited thereby, since simple modifications and variations can be made by those skilled in the art without departing from the scope of the invention.
Claims (8)
1. A natural enrichment method of anammox bacteria is characterized by comprising the following steps:
the method comprises the following steps: constructing a reaction device, wherein the reaction device can realize intermittent water inlet and outlet and provide an anoxic environment, and the reactor is a Sequencing Batch Reactor (SBR);
step two: feeding water into the reaction device at the first stage, adding a carbon source to enable the filler to form a film, and performing heterotrophic half-range denitrification, wherein the water fed into the reaction device at the first stage comprises nitrate nitrogen and ammonia nitrogen;
step three: and feeding water into the second stage of the reaction device, adding sulfur simple substance with the concentration of 0.05-0.2g/L into the water every time, inoculating sulfur autotrophic denitrification sludge, and replacing heterotrophic denitrification with sulfur autotrophic denitrification to realize natural enrichment of anaerobic ammonia oxidation, wherein the water fed into the second stage comprises nitrate nitrogen and ammonia nitrogen.
2. The method for naturally enriching the anammox bacteria according to claim 1, wherein the mass concentration ratio of carbon to nitrogen is 2.5/1-3.5/1 when the carbon source is added in the second step.
3. The method for naturally enriching anammox bacteria according to claim 1, wherein the reaction time of each period in the first stage of step two is 30-50min, and 200-280 periods are performed.
4. The method for naturally enriching anammox bacteria according to claim 1, wherein when the sulfur autotrophic denitrification sludge is inoculated in the third step, the sulfur autotrophic denitrification sludge is inoculated in a ratio of 1/5 to 1/10.
5. The method for naturally enriching the anaerobic ammonium oxidation bacteria according to claim 1, wherein the mass concentration ratio of the nitrate nitrogen to the ammonia nitrogen in the influent water obtained in the step three is 2/1-4/1; the reaction time of each period of the second stage is 5-6h, and the reaction is carried out for 80-120 periods.
6. The method for naturally enriching anammox bacteria according to claim 1,
the method further comprises the steps of:
step four: feeding water into the reaction device at the third stage, adding a sulfur simple substance with the concentration of 0.05-0.2g/L to strengthen the enrichment of anaerobic ammonium oxidation bacteria, wherein the proportion of ammonia nitrogen in the water fed into the third stage is greater than that in the water fed into the first stage and the water fed into the second stage; the reaction time of each period is 5-6h, and the reaction is carried out for 40-70 periods;
step five: and (3) feeding water into the fourth stage of the reaction device, adding a sulfur simple substance with the concentration of 0.05-0.2g/L into the water in each time to strengthen the enrichment of the anammox bacteria, wherein the proportion of ammonia nitrogen in the water fed into the fourth stage is greater than that in the water fed into the third stage, and the reaction time in each period is 5-6h, and carrying out 40-70 periods.
7. The method for natural enrichment of anammox bacteria according to claim 1, wherein the reaction temperature is 19-25 ℃ and the pH is 7.0-8.0 during the operation of the method.
8. The method for naturally enriching anammox bacteria according to claim 1, wherein the reactor is an SBR reactor, the anoxic environment providing device is a stirring paddle, the filler is polyurethane sponge filler, the filling ratio is 20-50%, and the drainage ratio is 40-60%.
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