CN114045213A - Concrete biological blockage culture apparatus permeates water - Google Patents

Concrete biological blockage culture apparatus permeates water Download PDF

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Publication number
CN114045213A
CN114045213A CN202110916424.2A CN202110916424A CN114045213A CN 114045213 A CN114045213 A CN 114045213A CN 202110916424 A CN202110916424 A CN 202110916424A CN 114045213 A CN114045213 A CN 114045213A
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pervious concrete
biological
control
culture
regulation
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Inventor
蹇守卫
魏博
李相国
谭洪波
黄健
吕阳
王威振
雷宇婷
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Wuhan University of Technology WUT
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Wuhan University of Technology WUT
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Priority to CN202110916424.2A priority Critical patent/CN114045213A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Computer Hardware Design (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of biological culture, and provides a pervious concrete biological blockage culture device which comprises a box body, a regulation and control assembly and a control system, wherein the regulation and control assembly is used for controlling the environmental change in the box body, the control system is used for controlling the regulation and control assembly to fully automatically regulate the environment, and a biological culture area for culturing microorganisms and an image acquisition assembly for observing the conditions of the microorganisms on a sample platform are arranged in the box body. The pervious concrete biological blockage culture device can simulate the environment of pervious concrete biological growth and observe the condition of the pervious concrete biological growth so as to find out the growth condition and the growth period of the pervious concrete biological and provide necessary reference data for solving biological blockage.

Description

Concrete biological blockage culture apparatus permeates water
Technical Field
The invention relates to the technical field of biological culture, in particular to a pervious concrete biological blockage culture device.
Background
The permeable material is one of the most main building materials for building the sponge city, has the functions of maintaining water resource balance, improving air permeability, realizing surface temperature and humidity regulation, relieving urban heat island effect, absorbing noise brought by daily life, improving ecological environment and the like, and has great significance for promoting the building of the sponge city.
In spring and summer seasons of China, the climate is humid, the temperature is proper, a large amount of microorganisms, moss, algae and other organisms can propagate in pores of a permeable material pavement, the pores can be plugged within 1-2 years, so that the permeable function of the material is lost, potential safety hazards can be brought to pedestrians, and the biological plugging becomes a serious obstacle to the further development of a sponge city. The existing method for solving the biological blockage is generally spraying a medicament, has an inhibiting effect on the growth of the medicament, and has great harm to the environment. At present, research on biological plugging of permeable materials is limited at home and abroad, and no related biological culture device exists. When the permeable material biological plugging experiment is carried out in a natural environment, external environment factors are not controllable, and because the growth of organisms needs proper temperature, humidity, illumination intensity and illumination time, the optimal growth conditions are difficult to meet under natural conditions, so that the experiment accuracy and the growth period of the organisms are greatly influenced.
Disclosure of Invention
The invention aims to provide a pervious concrete biological plugging culture device which can at least solve part of the defects in the prior art.
In order to achieve the above purpose, the embodiments of the present invention provide the following technical solutions: the utility model provides a pervious concrete biological blocking culture device, includes box, control regulation and control subassembly and the control of environmental change in the box regulation and control subassembly is with the control system of full automatically regulated environment, it cultivates the district and is used for observing to have the biology that is used for cultivateing the microorganism in the box the image acquisition subassembly of the situation of microorganism on the sample platform.
Further, the regulation and control assembly comprises a spraying device, the spraying device is used for spraying moisture and nutrient solution for the growth of microorganisms, and the control system controls the spraying time and time interval of the spraying device.
Further, the regulation and control subassembly includes heating device, heating device is used for the temperature in the control box, control system control the temperature of heating device heating.
Further, the heating temperature range of the heating device is 5-60 ℃.
Further, the image acquisition assembly comprises a camera, the camera is installed above the biological culture area, and the camera is connected with the control system.
Furthermore, the biological culture area is provided with a reticular clapboard for placing permeable material test blocks, the reticular clapboard is transversely arranged in the box body, and the reticular clapboard is separated from the bottom of the box body.
Further, the regulation and control assembly comprises a fluorescent lamp and is used for simulating sunlight.
Further, the regulation and control assembly further comprises an illuminometer for detecting the illumination intensity of the fluorescent lamp, and the illuminometer feeds monitored signals back to the control system in real time.
Further, the device also comprises an atmosphere detector for detecting the temperature, the humidity, the oxygen concentration and the carbon dioxide concentration in the box body, wherein the atmosphere detector feeds monitored signals back to the control system in real time.
Further, the box body also comprises an exhaust device used for exhausting gas in the box body, and the box body also comprises an air inlet corresponding to the exhaust device.
Compared with the prior art, the invention has the beneficial effects that: a pervious concrete biological blockage culture device can simulate the environment of pervious concrete biological growth and observe the condition of the pervious concrete biological growth so as to find out the growth condition and the growth period of the pervious concrete biological and provide necessary reference data for solving biological blockage.
Drawings
FIG. 1 is a front view of a pervious concrete biological plugging culture device according to an embodiment of the present invention;
FIG. 2 is a top view of a pervious concrete biological plugging culture device according to an embodiment of the present invention;
FIG. 3 is a bottom view of a pervious concrete biological plugging culture device according to an embodiment of the present invention;
in the reference symbols: 1-a box body; 2-a fluorescent lamp; 3-an exhaust device; 4-a heating device; 5-test block; 6-a sample platform; 7-water outlet; 8-illuminometer; 9-atmosphere detector; 10-a camera; 11-a spraying device; 12-a box door.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, 2 and 3, an embodiment of the present invention provides a pervious concrete biological plugging culture apparatus, including a box 1, a regulation and control component for controlling the environmental change in the box 1, and a control system for controlling the regulation and control component to fully automatically regulate the environment, wherein the box 1 has a biological culture area for culturing microorganisms therein and an image acquisition component for observing the conditions of the microorganisms on the sample platform 6. In this embodiment, the device can simulate the environment of the biological growth of the pervious concrete and observe the biological growth condition of the pervious concrete, so as to find out the growth condition and the growth period of the pervious concrete, and provide necessary reference data for solving the biological blockage. Specifically, when using, will permeate water concrete organism and place the biological cultivation district and cultivate, then observe living beings growth in real time through the image acquisition subassembly, during the observation period, adopt the regulation and control subassembly to adjust the environmental change in the box 1, further can adopt control system to control the regulation and control subassembly to realize full automatically regulated. The control system can be used for presetting control conditions such as illumination conditions, temperature and humidity conditions and the like by a computer end control system, then controlling the action of the regulation and control assembly by preset values, and controlling each part controlled by the regulation and control assembly to perform corresponding regulation and control actions so as to achieve the purpose of changing the environment.
Referring to fig. 1, 2 and 3 as an optimization solution of the embodiment of the present invention, the regulating and controlling assembly includes a spraying device 11, the spraying device 11 is used for spraying moisture and nutrient solution for microorganism growth, and the control system controls the spraying time and time interval of the spraying device 11. In this embodiment, the spraying device 11 is used to spray water and nutrient solution, which can provide water and nutrient for biological growth, the sprayed liquid is in a mist form, which can uniformly wet the surface of the test block 5, and the spraying time and time interval can be controlled and adjusted by the control system. The spraying time is preferably 30 s/time, and the time interval is 3 h/time.
Referring to fig. 1, fig. 2 and fig. 3 as an optimized solution of the embodiment of the present invention, the regulating and controlling assembly includes a heating device 4, the heating device 4 is used for controlling the temperature inside the box body 1, and the control system controls the temperature heated by the heating device 4. In the embodiment, the heating device 4 is used to provide the necessary temperature in the box 1, the temperature range is preferably 5-60 ℃, and preferably, the heating device 4 can be a resistance heater and is installed on both sides of the box 1, which is controlled by the control system.
Referring to fig. 1, 2 and 3 as an optimized solution of the embodiment of the present invention, the image capturing assembly includes a camera 10, the camera 10 is installed above the biological culture area, and the camera 10 is connected to the control system. In this embodiment, a 500 ten thousand pixel high-definition wireless camera 10 with a waterproof function is adopted and installed in the middle of a fluorescent lamp 2 tube above a culture area, four fluorescent lamps are provided and connected with a control system to collect images of the test block 5. The growth state of the bryophyte can be monitored in real time.
Referring to fig. 1, 2 and 3 as an optimized scheme of the embodiment of the present invention, the biological culture area is provided with a mesh partition board capable of placing a permeable material test block 5, the mesh partition board is transversely arranged in the box body 1, and the mesh partition board is spaced from the bottom of the box body 1. In this embodiment, the reticular baffle that adopts can be stainless steel, and life is longer, can place permeable material test block 5, and the distance of baffle apart from the bottom of the case is between 15 ~ 20 cm.
Referring to fig. 1, 2 and 3 as an optimization scheme of the embodiment of the present invention, the adjusting and controlling assembly includes a fluorescent lamp 2 for simulating sunlight. The regulation and control assembly further comprises an illuminometer 8 for detecting the illumination intensity of the fluorescent lamp 2, and the illuminometer 8 feeds monitored signals back to the control system in real time. In this embodiment, the fluorescent lamp 2 is a tubular LED lamp, two lamp tubes are installed on the top of the box body 1, the fluorescent lamp 2 has a waterproof function, sunlight can be simulated, and the illumination intensity can be freely adjusted within the range of 100-5000 lux. The illuminometer 8 has a waterproof function, the detection range of the illumination intensity is 100-10000 lux, and the illumination intensity can be fed back to a computer end control system in real time.
Referring to fig. 1, 2 and 3 as an optimized scheme of the embodiment of the present invention, the apparatus further includes an atmosphere detector 9 for detecting the temperature, humidity, oxygen concentration and carbon dioxide concentration in the box 1, and the atmosphere detector 9 feeds back a monitored signal to the control system in real time. In this embodiment, the atmosphere detector 9 is installed on the above-mentioned netted baffle, and wherein the temperature detection range is 5 ~ 60 ℃, and the humidity detection range is 0 ~ 100% RH, still can detect the incasement oxygen and carbon dioxide concentration, and the detection range is 0 ~ 100% vol, and humiture data can feed back to computer end control system in real time.
Referring to fig. 1, 2 and 3 as an optimized solution of the embodiment of the present invention, the apparatus further includes an exhaust device 3 for exhausting gas in the box body 1, and the box body 1 further has an air inlet corresponding to the exhaust device 3. In the present embodiment, the exhaust means 3 is an exhaust pump, which is installed outside the case 1 and connected to an exhaust hole. 1 air inlet hole is seted up to the exhaust hole offside, and exhaust hole and air inlet hole diameter are 3 ~ 5 cm. The exhaust device 3 is controlled by a computer-side control system.
As an optimized scheme of the embodiment of the invention, please refer to fig. 1, fig. 2 and fig. 3, the device further comprises a water outlet 7, wherein the water outlet 7 is arranged at the bottom of the box body 1, has a diameter of 1-2 cm, and can be connected with a water outlet hose. The box body 1 can be washed by clean water and is discharged from the water outlet 7, which is very convenient.
As an optimization scheme of the embodiment of the invention, the computer-side control system can control the working states of the fluorescent lamp 2, the heater and the exhaust pump in the box body 1 in real time according to the temperature and humidity data and the illumination intensity. The environmental parameters in the incubator can be fully automatically adjusted by a computer-side control system. The invention principle is as follows: the required temperature range, humidity range, oxygen and carbon dioxide concentration range, illumination intensity range and illumination time are set, the temperature and humidity sensor and the illuminometer 8 feed back real-time monitoring data to the control system, and the control system controls the operation of the heater, the exhaust pump and the fluorescent lamp 2 until the required temperature and humidity atmosphere range and illumination intensity range are reached. The box door 12 is opened, the test block 5 is placed on the sample platform 6, then the box door 12 is closed, and then the heating device 4 and the exhaust device 3 start to work until the required parameter range is reached, and water in the device can be discharged through the water outlet at the bottom. After a certain period of growth, the computer can shoot the growth state of the living being through the wireless high-definition camera 10 of the information acquisition system.
As an optimized scheme of the embodiment of the invention, the box body 1 is mainly composed of heat preservation and insulation materials, and the heat conductivity coefficient is 0.01-0.02W/(mk). The box body 1 is 1.2-1.5 m in length, 0.5-0.6 m in width, 0.5-0.6 m in height and 1-2 cm in thickness.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. The utility model provides a concrete biological blocking culture apparatus permeates water which characterized in that: including the box, control regulation and control subassembly and control of environmental change in the box the control system of regulation and control subassembly with full automatically regulated environment, have the biological culture district that is used for cultivateing the microorganism in the box and be used for observing the image acquisition subassembly of the condition of microorganism on the sample platform.
2. The pervious concrete biological plugging culture device of claim 1, wherein: the regulation and control subassembly includes sprinkler, sprinkler is used for spraying moisture and sprays the nutrient solution that supplies microorganism to grow, control system controls sprinkler's spraying time and time interval.
3. The pervious concrete biological plugging culture device of claim 1, wherein: the regulation and control subassembly includes heating device, heating device is used for the temperature in the control box, control system control the temperature of heating device heating.
4. The pervious concrete biological plugging culture device of claim 3, wherein: the heating temperature range of the heating device is 5-60 ℃.
5. The pervious concrete biological plugging culture device of claim 1, wherein: the image acquisition assembly comprises a camera, the camera is installed above the biological culture area, and the camera is connected with the control system.
6. The pervious concrete biological plugging culture device of claim 1, wherein: the biological culture area is provided with a reticular clapboard capable of placing permeable material test blocks, the reticular clapboard is transversely arranged in the box body, and the reticular clapboard is separated from the bottom of the box body.
7. The pervious concrete biological plugging culture device of claim 1, wherein: the regulation and control assembly comprises a fluorescent lamp and is used for simulating sunlight.
8. The pervious concrete biological plugging culture device of claim 7, wherein: the regulation and control assembly further comprises an illuminometer for detecting the illumination intensity of the fluorescent lamp, and the illuminometer feeds monitored signals back to the control system in real time.
9. The pervious concrete biological plugging culture device of claim 1, wherein: the atmosphere detector is used for detecting the temperature, the humidity, the oxygen concentration and the carbon dioxide concentration in the box body, and feeds monitored signals back to the control system in real time.
10. The pervious concrete biological plugging culture device of claim 1, wherein: the box body is also provided with an air inlet corresponding to the exhaust device.
CN202110916424.2A 2021-08-11 2021-08-11 Concrete biological blockage culture apparatus permeates water Pending CN114045213A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160143227A1 (en) * 2013-07-08 2016-05-26 Forteco B.V. Cultivating device
CN207452120U (en) * 2017-11-17 2018-06-05 山东鲁健检测技术服务有限公司 A kind of microbiological incubator
CN108157172A (en) * 2017-11-13 2018-06-15 北京建筑大学 A kind of microphyte incubator
CN208064001U (en) * 2018-02-28 2018-11-09 北京农业信息技术研究中心 One plant growth network push device
CN208328026U (en) * 2018-04-16 2019-01-04 安徽省金标准检测研究院有限公司 A kind of food inspection microbiological incubator

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160143227A1 (en) * 2013-07-08 2016-05-26 Forteco B.V. Cultivating device
CN108157172A (en) * 2017-11-13 2018-06-15 北京建筑大学 A kind of microphyte incubator
CN207452120U (en) * 2017-11-17 2018-06-05 山东鲁健检测技术服务有限公司 A kind of microbiological incubator
CN208064001U (en) * 2018-02-28 2018-11-09 北京农业信息技术研究中心 One plant growth network push device
CN208328026U (en) * 2018-04-16 2019-01-04 安徽省金标准检测研究院有限公司 A kind of food inspection microbiological incubator

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
高峰 等: "《建筑材料科学基础》", 同济大学出版社, pages: 357 *

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