CN113476509A - Application of peach bark extract in preventing and treating ichthyophthiriasis of freshwater fish - Google Patents
Application of peach bark extract in preventing and treating ichthyophthiriasis of freshwater fish Download PDFInfo
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- 235000006040 Prunus persica var persica Nutrition 0.000 title claims abstract description 49
- 239000000284 extract Substances 0.000 title claims abstract description 33
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 16
- 239000013505 freshwater Substances 0.000 title claims abstract description 9
- 240000006413 Prunus persica var. persica Species 0.000 title 1
- 244000144730 Amygdalus persica Species 0.000 claims abstract description 47
- 210000003812 trophozoite Anatomy 0.000 claims abstract description 16
- 230000002147 killing effect Effects 0.000 claims abstract description 13
- 244000062645 predators Species 0.000 claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 26
- 241000238631 Hexapoda Species 0.000 claims description 19
- 239000000469 ethanolic extract Substances 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 5
- 240000005809 Prunus persica Species 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 235000011446 Amygdalus persica Nutrition 0.000 claims description 3
- 238000005538 encapsulation Methods 0.000 claims description 3
- 241000558306 Gynocardia odorata Species 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 10
- 208000031513 cyst Diseases 0.000 abstract description 9
- 229920001983 poloxamer Polymers 0.000 abstract description 6
- 241000248484 Ichthyophthirius Species 0.000 abstract description 3
- 238000000338 in vitro Methods 0.000 abstract description 3
- 230000000749 insecticidal effect Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 3
- 230000006806 disease prevention Effects 0.000 abstract 1
- 231100000956 nontoxicity Toxicity 0.000 abstract 1
- 241000248482 Ichthyophthirius multifiliis Species 0.000 description 9
- 229940079593 drug Drugs 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 241000411851 herbal medicine Species 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- 241000219109 Citrullus Species 0.000 description 5
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 238000011160 research Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000009304 pastoral farming Methods 0.000 description 3
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- 206010011732 Cyst Diseases 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 241000222640 Polyporus Species 0.000 description 2
- 241001275931 Rhodeus ocellatus Species 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000002816 gill Anatomy 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000208140 Acer Species 0.000 description 1
- 241000223782 Ciliophora Species 0.000 description 1
- 241000723346 Cinnamomum camphora Species 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000219122 Cucurbita Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000565359 Fraxinus chinensis Species 0.000 description 1
- 235000013717 Houttuynia Nutrition 0.000 description 1
- 240000000691 Houttuynia cordata Species 0.000 description 1
- 241000334160 Isatis Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010027982 Morphoea Diseases 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241000500437 Plutella xylostella Species 0.000 description 1
- 241001077936 Polymnia Species 0.000 description 1
- 244000134540 Polymnia sonchifolia Species 0.000 description 1
- 235000003406 Polymnia sonchifolia Nutrition 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000519995 Stachys sylvatica Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 208000028454 lice infestation Diseases 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000004202 respiratory function Effects 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 201000010153 skin papilloma Diseases 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000002477 vacuolizing effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Alternative & Traditional Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
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Abstract
The invention discloses application of a peach bark extract in preventing and treating ichthyophthiriasis of freshwater fish, belonging to the technical field of fishery disease prevention and treatment. The in vitro insecticidal test result shows that: the peach bark extract has good killing effects on predators, trophozoites and cysts of the pluronic ichthyophthirius, and has a dose-effect relationship, so that the peach bark extract can prevent and treat the pluronic ichthyophthiriasis of the freshwater fish by killing worms. The peach bark is used as the traditional Chinese medicine in China, the extract of the peach bark has the characteristics of safety and no toxicity to water body environment and aquatic organisms, and has great application prospect in the field of novel green fishery medicine.
Description
Technical Field
The invention belongs to the technical field of fishery disease control, and particularly relates to application of a peach (Prunus persica) bark extract in control of ichthyophthiriasis of freshwater fish.
Background
The ichthyophthirius multifiliis a ciliate protozoan, widely parasitizes on the body surfaces, gills and other parts of various freshwater fishes, can form macroscopic white spots, and causes diseases commonly known as white spot diseases. After the fish is infected with the ichthyophthirius multifiliis, the diseased fish is scorched and uneasy, the appetite is reduced, pathological symptoms such as tissue hyperplasia, necrosis and the like appear at parasitic parts, and when the gill part is seriously infected, the respiratory function of the fish is damaged, and finally the fish dies. At present, the polycapillaria is mainly prevented and treated by formalin, copper sulfate and other chemical drugs in the breeding production, but the use of the drugs faces to the generation of drug resistance, the toxic effect on fishes, the water environment pollution and other problems. Therefore, the search for safe and effective alternative drugs is a hot problem in the research of the ichthyophthiriasis.
Chinese herbal medicine is rich in resources in China, has the advantages of nature, low toxicity, environmental friendliness and the like, and is an important resource for developing green medicines. As early as in northern Song of China, "the object-oriented emotion" has a Chinese herbal medicine therapy of fish thin and white-point generation, named louse, which is cured by adding maple bark into water. The Ming Dynasty started in the book of agricultural and political sciences, and the louse was found, "pine hair passed through the pond, and floating was removed". Studies have shown that the "louse" referred to herein is a melon pest. In recent years, researches show that various Chinese herbal medicines have a killing effect on the ichthyophthirius multifiliis, and the Chinese herbal medicines have a huge application prospect in the effect of resisting the ichthyophthirius multifiliis.
Peach (Prunus persica) is a traditional Chinese herbal medicine in China, but the research on the drug effect is relatively deficient. In recent years, researches show that the peach bark has a certain effect on treating intractable diarrhea of children and flat warts of people. In the aspect of insect resistance, peach bark and poplar bark are decocted to obtain concentrated juice which can be used for treating pig pediculosis. However, no relevant report of the application of the peach bark in the prevention and treatment of aquatic diseases exists at present.
Disclosure of Invention
The invention aims to provide application of a peach bark extract in preventing and treating ichthyophthiriasis of freshwater fish.
The purpose of the invention is realized by the following scheme:
the in vitro insecticidal test result shows that: the peach bark extract has good killing effects on predators, trophozoites and cysts of the pluronic ichthyophthirius, and has a dose-effect relationship, so that the peach bark extract can prevent and treat the pluronic ichthyophthiriasis of the freshwater fish by killing worms.
The cortex Persicae extract is an absolute ethanol extract of cortex Persicae.
Preferably, the preparation method of the extract comprises the following steps: pulverizing cortex Persicae into powder, adding anhydrous alcohol, extracting, concentrating the extractive solution, and drying.
Further preferably, the absolute ethyl alcohol is used in an amount of 5-20 times the weight of the bark powder.
Further preferably, the temperature of the warm immersion is 40-70 ℃.
Further preferably, the number of times of extraction is 2-5 times, and the time of each extraction is 0.5-3 hours.
The invention further provides a method for killing the Polymnia sonchifolia, which comprises the following steps: the bark extract of peach is added to the insect solution of the Polychachis bicolor.
Preferably, different amounts of the peach bark extract are applied at different stages of life history of the chaulmoogra, wherein the peach bark extract is applied at a final concentration of 6.2mg/L at the stage of predation; applying a final concentration of 25mg/L of the bark extract of peach at the trophozoite stage; the application of the peach bark extract at a final concentration of 100mg/L in the encapsulation stage can completely kill the insects.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the peach bark serving as the medicine raw material is traditional Chinese herbal medicine, resources are rich in China, the extraction process is simple and convenient, and plant extracts are easy to obtain.
(2) The peach bark in the invention is widely used in forestry and animal husbandry, is a safe and nontoxic drug raw material, and is safer in fishery culture compared with chemical drugs.
(3) The invention reports that the peach bark extract has obvious pharmaceutical activity on the small fruit ichthyophthirius multifiliis for the first time, and the peach bark extract can kill the small fruit ichthyophthirius multifiliis in 100% in various stages of life history within an effective concentration range.
Detailed Description
The present invention will be described in detail with reference to specific examples, but the embodiments of the present invention are not limited thereto.
The materials used in the following examples are: rhodeus ocellatus (Rhodeus ocellatus) suffering from ichthyophthiriasis was collected from the aquatics of university of agriculture in china.
Before the test begins, the applicant selects 75 Chinese herbal medicines such as isatis root, camphor tree leaves, peach bark, Chinese ash bark, houttuynia and the like, and the killing effect on the grazing bodies of the ichthyophthirius multifiliis is observed under the in vitro condition after the ethanol extraction, so that the screened peach bark has the best insecticidal effect, and the effect of other Chinese herbal medicines is not ideal.
The preparation method of the peach bark extract comprises the following steps: drying the purchased peach bark in a 55 ℃ oven to constant weight, crushing by using a crusher, and sieving by using a 20-mesh sieve to obtain the medicine powder. Accurately weighing 20g of medicine powder, adding 200mL of absolute ethyl alcohol according to the proportion of 1:10, and leaching for 2 times in a 45 ℃ water bath kettle for 2 hours each time. The supernatants from the 2 leaches were combined and the residual drug powder inside was filtered. Concentrating the filtrate under reduced pressure with rotary evaporation concentrator, drying the concentrated product in oven at 55 deg.C to remove residual ethanol, and collecting the ethanol extract of cortex Persicae.
Accurately weighing 0.1g of crude extract, fully dissolving with 10mL of absolute ethanol, and adding 90mL of distilled water to make the final concentration of the peach bark extract be 1g/L and the ethanol content be 10%.
The collecting method of the polyporus cucurbitae worms comprises the following steps: the method comprises the steps of gently scraping a plurality of water melon worm trophozoites from the surface of a diseased fish body by using a cell scraper into a culture dish filled with sterile aerated water, sucking the swimming worm bodies into a clean culture dish by using a plastic suction pipe after the trophozoites start to swim in the water, repeatedly eluting for 2-3 times, collecting part of trophozoites for a subsequent efficacy evaluation experiment, placing the rest trophozoites and the culture dish into an incubator at 25 ℃ for incubation, and collecting insect liquid after about 12-14 hours, wherein a large amount of swimming predators are in the culture dish and are used for the subsequent efficacy evaluation experiment.
Example 1: the peach bark extract has the effect of killing the pluronic ichthyophthirius multifiliis bodies.
Sucking 100 mu L of the pluronic ichthyophthirius fasciatus grazing body liquid and about 60 insect bodies into a 96-well plate, totaling 5 experimental groups and 2 control groups, wherein each group is provided with 3 parallel groups, and totaling 2100uL of insect liquid. The stock solutions were diluted to obtain a concentration gradient of 12.5, 6.2, 3.1, 1.6 and 0.8mg/L of the peach bark extract, and an equal volume (100. mu.L) was added to each experimental group to give final concentrations of 6.2, 3.1, 1.6, 0.8 and 0.4mg/L, respectively. The ethanol control group was added with 100. mu.L of 0.125% absolute ethanol, and the blank control group was added with 100. mu.L of aerated water. And (3) culturing the 96-well plate in an incubator at 25 ℃, counting the mortality of each group of predators after 4 hours, and observing the state of the insect body by a microscope.
The results show that: when the concentration of the peach bark ethanol extract is 6.25mg/L, 100% of the plutella xylostella predators can be killed within 4 hours; when the concentration is reduced to 3.1mg/L, 44.9 percent of predators can be killed in 4 hours; when the concentration is further reduced to 0.4mg/L, the peach bark ethanol extract can still kill 4.6 percent of predators within 4 hours. Control group did not die on predators. Microscopic observation shows that after the treatment of the medicine, the predator body loses the motor ability, cytoplasm does not flow, cell membranes are ruptured, and the polypide dies.
Example 2: the peach bark extract has the effect of killing the polyporus cucurbitus trophozoites.
Sucking 1mL of the water melon insect nourishing body insect liquid and about 30 insect bodies into a 12-hole plate, totaling 6 experimental groups and 2 control groups, setting 3 parallel groups in each group, and totaling 24mL of the insect liquid. The drug stock solution was diluted to obtain ethanol extracts of peach bark with concentration gradients of 50, 25, 12.5, 6.25, 3.1 and 1.5mg/L, and an equal volume (1mL) was added to each experimental group to give final concentrations of 25, 12.5, 6.25, 3.1, 1.5 and 0.8mg/L, respectively. The ethanol control group was added with 1mL of 0.5% absolute ethanol, and the blank control group was added with 1mL of aerated water. Culturing 12-hole plate in 25 deg.C incubator for 6 hr, counting the death rate of each group of trophozoite, and observing the state of insect body with microscope.
The results show that: when the concentration of the peach bark ethanol extract is 25.0mg/L, 100% of the water melon worms can be killed within 6 h; when the concentration is reduced to 6.2mg/L, 6.0 percent of trophozoite can be killed within 6 h; when the concentration is further reduced to 3.1mg/L, the ethanol extract of the peach bark has no effect on the trophozoite within 6 h. Control group did not die of trophozoite. Microscopic observation shows that after the treatment of the medicine, the trophozoite loses the motor capacity, vacuolization appears in cytoplasm, partial cell membranes of the polypide are broken, and the polypide dies.
Example 3: the peach bark extract has effect of killing the cyst of the ichthyophthirius multifiliis.
Sucking 1mL of the water melon insect nourishing body insect liquid and about 30 insect bodies into a 12-hole plate, totaling 6 experimental groups and 2 control groups, setting 3 parallel groups in each group, and totaling 24mL of the insect liquid. After further incubation of the 12-well plates at 25 ℃ for 6h, the trophozoites in each well entered the encapsulation stage. The drug stock solution was diluted to obtain ethanol extracts of peach bark with concentration gradients of 200, 100, 50, 25, 12.5 and 6.25mg/L, and an equal volume (1mL) was added to each experimental group to give final concentrations of 100, 50, 25, 12.5, 6.25 and 3.1mg/L, respectively. The ethanol control group was added with 1mL of 2% absolute ethanol, and the blank control group was added with 1mL of aerated water. Culturing 12-hole plate in 25 deg.C incubator for 6 hr, counting the death rate of each group of capsules, and observing the state of insect body with microscope.
The results show that: when the concentration of the peach bark ethanol extract is 100mg/L, 100% of the water melon seeds can be killed within 6 hours; when the concentration is 25mg/L, 65.6 percent of capsules can be killed within 6 h; when the concentration is reduced to 12.5mg/L, the peach bark ethanol extract can kill 4.4 percent of cysts within 6 hours; when the concentration is 6.25mg/L, the microcapsule has no killing effect on the cysts. Control group died without cysts. Microscopic observation shows that after the medicine is treated, the entomogenous bodies inside the cysts lose the movement capability and die, vacuolation occurs in cells, and the division of the cysts is stopped.
TABLE 1 Effect of peach bark ethanol extract on Pest-grazing, trophozoite and cyst of Polymnia cucurbita
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (8)
1. Application of peach (Prunus persica) bark extract in preventing and treating ichthyophthiriasis of freshwater fish.
2. The use of claim 1, wherein: the cortex Persicae extract is an absolute ethanol extract of cortex Persicae.
3. The use of claim 2, wherein the extract is prepared by a method comprising: pulverizing cortex Persicae into powder, adding anhydrous alcohol, extracting, concentrating the extractive solution, and drying.
4. Use according to claim 3, characterized in that: the amount of the absolute ethyl alcohol is 5-20 times of the weight of the peach bark powder.
5. Use according to claim 3, characterized in that: the temperature of the warm immersion is 40-70 ℃.
6. Use according to claim 3, characterized in that: the extraction times are 2-5 times, and each extraction time is 0.5-3 hr.
7. A method for killing the Polychachis bicolor is characterized in that: the bark extract of peach is added to the insect solution of the Polychachis bicolor.
8. The method for killing the Polychachis bicolor according to claim 7, wherein: applying different amounts of the peach bark extract at different life history stages of the chaulmoogra, wherein the peach bark extract with the final concentration of 6.2mg/L is applied at the predator stage; applying a final concentration of 25mg/L of the bark extract of peach at the trophozoite stage; the application of the peach bark extract at a final concentration of 100mg/L in the encapsulation stage can completely kill the insects.
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| CN202110929146.4A CN113476509A (en) | 2021-08-13 | 2021-08-13 | Application of peach bark extract in preventing and treating ichthyophthiriasis of freshwater fish |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115517207A (en) * | 2022-09-26 | 2022-12-27 | 中国科学院水生生物研究所 | Method and device for evaluating in-vivo drug killing effect of ichthyophthirius multifiliis |
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2021
- 2021-08-13 CN CN202110929146.4A patent/CN113476509A/en active Pending
Non-Patent Citations (1)
| Title |
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| 张珂: "养鱼防病技巧多", 《现代农业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115517207A (en) * | 2022-09-26 | 2022-12-27 | 中国科学院水生生物研究所 | Method and device for evaluating in-vivo drug killing effect of ichthyophthirius multifiliis |
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