CN111869672B - Bactericidal composition and application thereof - Google Patents
Bactericidal composition and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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Abstract
The invention provides a sterilization composition and application thereof. The main active ingredients of the bactericidal composition comprise two components, namely: biguanide trioctylphenyl sulfonate; the second component: the mass ratio of the first component to the second component is 1: 20-20: 1, and the total mass of the two components accounts for 5-80% of the total mass of the composition. The composition has the advantages of reduced amount, increased efficiency, low toxicity, little harm to human, livestock and ecological environment, delay of generation and development of drug resistance of pathogenic agents, good prevention and treatment effects on scab, powdery mildew, black spot and brown spot fungal diseases, and wide application prospect.
Description
The application is a divisional application of an invention patent application with the title of 'a bactericidal composition and application thereof' on application date 2018.05.18, application number 201810481626.7.
Technical Field
The invention relates to the field of bactericides, and in particular relates to a bactericidal composition containing iminoctadine tris (component one), xinjunan acetate, phenazine alpha-2 carboxylic acid, prothioconazole or trifloxystrobin (component two).
Background
Biguanide trioctylphenyl sulfonate, chemical name: 1, 1-octyl methylene biguanide trioctylphenyl sulfonate. The biguanide trioctyl benzene sulfonate is a broad-spectrum and low-toxicity bactericide, inhibits the biosynthesis of germ lipid compounds, has strong local permeability, and has high growth inhibition activity on pathogenic ascomycetes and adelomycetes.
The Xinjunan acetate is a quaternary ammonium salt bactericide, and achieves the aim of sterilization by destroying various pathogens to obtain cell membranes, coagulating protein, preventing respiration and enzyme activity and the like. Has certain systemic and osmotic effects, and has obvious killing and inhibiting effects on various plant fungi, bacteria and viruses causing crop diseases.
The phenazine alpha-2 carboxylic acid can rapidly dissolve cell walls of harmful fungi and bacteria, directly kill pathogenic bacteria, generate cell antibodies, interfere synthesis of the cell walls of the pathogenic bacteria, and inhibit generation and development of diseases; killing harmful germs, dissolving toxin secreted by germs, dredging plant microcirculation, and smoothing nutrient delivery; inducing plants to generate disease resistance, forming a strong disease resistance defense system and being effective to various fruit tree fungal diseases.
The prothioconazole not only has good systemic action and excellent protection, treatment and eradication activity, but also has long lasting period. Compared with triazole bactericides, prothioconazole has a wider bactericidal spectrum.
The trifloxystrobin is a novel medicament for specially treating powdery mildew, and the bactericidal strength of the trifloxystrobin is improved by 5 times compared with that of trifloxystrobin and is improved by 10 times compared with that of pyraclostrobin by combining a nano DV technology and a targeted pharmaceutical technology; the medicament has the characteristics of super-strong permeability, quick absorption, quick conduction, long lasting period and the like, and has very obvious use effect in areas with resistance to powdery mildew on pyraclostrobin, aether phenolsulfonate, kresoxim-methyl, polyoxin, myclobutanil and the like; the product is safe to various crops, can be used in flowering phase, does not pollute fruits and does not harm natural enemies.
The pear scab is also called scab, and the main pear area is common, strong in popularity and large in loss and is harmful from the flower falling period to the fruit mature period. The damage of young fruit is abnormal fruit, and the fruit is suberized in the growth period. The leaves are damaged and spread along the veins to form black mildew spots and even black mildew layers. The symptoms of petioles and fruit stalks are similar, black oval pit spots appear, the diseased part is covered with black mold, the disease is constricted, and the leaves or the fruits fall early due to dehydration and drying.
The powdery mildew of pears is a fungal disease in pear producing areas, and a large amount of white powdery spots appear on leaves when the disease occurs, and the growth of plants in the next year is influenced when the disease occurs seriously.
The black spot of the pear tree is one of important diseases on the pear tree. The western pears, the crisp pears and the snow pears are most susceptible to diseases, and early fallen leaves and young shoots are withered when the diseases are serious, so that fruit cracking and early fallen fruits are caused, the tree vigor is seriously weakened, and the growth vigor and the disease resistance of the tree body are reduced.
Cercospora leaf spot, also known as pear leaf spot and pear leaf spot blight, mainly damages leaves and fruits, and causes pear leaf scorching and a large amount of fallen leaves in severe cases.
Disclosure of Invention
The invention aims to provide a bactericidal composition which has the advantages of reducing weight, improving efficiency, delaying the generation and development of drug resistance of pathogens, reducing the risk of resistance of pathogenic bacteria to a single bactericide and prolonging the service life of the bactericide.
In order to achieve the purpose of the invention, the invention provides a bactericidal composition which mainly comprises two active components:
the composition one is as follows: biguanide trioctylphenyl sulfonate;
the second component: one or more of Xinjunan acetate, phenazine alpha-2 carboxylic acid, prothioconazole and trifloxystrobin.
Further, the mass ratio of the first component to the second component in the bactericidal composition is 1: 20-20: 1; preferably 1:15 to 15:1, more preferably 1:10 to 10:1, more preferably 1:8 to 8:1, still more preferably 1:5 to 5:1, and most preferably 1: 1. For example, the mass ratio of component one to component two can be 1:20, 20:1, 1:15, 15:1, 1:10, 10:1, 1:8, 8:1, 1:5, 5:1, or 1: 1.
The invention also provides a bactericide which can be prepared from the bactericidal composition independently or prepared from the bactericidal composition and an auxiliary agent or a carrier which can be used in the field of pesticides.
The auxiliary agent comprises one or more than two of dispersing agent, lubricant, binder, dispersing agent, spreader, disintegrant, defoamer, emulsifier, wetting agent, thickener, preservative, stabilizer, antifreeze or cosolvent.
The bactericide can be prepared into various dosage forms available in the field of pesticides, and is preferably water dispersible granules, aqueous emulsion or microemulsion.
Further, in the bactericide, the total mass of the component one and the component two accounts for 5-80% of the total mass of the bactericide, and preferably 20-60%.
When the bactericide is a water dispersible granule, the bactericide can comprise one or more than two of the following auxiliary agents: A. dispersing agent: one or more of polycarboxylate, lignosulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylbenzene sulfonate calcium salt, naphthalene sulfonic acid formaldehyde condensate sodium salt, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ester and glycerin fatty acid ester polyoxyethylene ether; B. wetting agent: one or more of sodium dodecyl sulfate, calcium dodecyl benzene sulfonate, nekal BX, wetting penetrant F, alkyl naphthalene sulfonate, polyoxyethylene triphenyl ethylene phenyl phosphate, Chinese honeylocust fruit powder, silkworm excrement and soapberry powder; C. disintegrating agent: one or more of bentonite, urea, ammonium sulfate, aluminum chloride, citric acid, succinic acid, sodium bicarbonate and light calcium carbonate; D. the thickening agent is: one or more of xanthan gum, hydroxymethyl cellulose, hydroxyethyl cellulose, methyl cellulose, magnesium aluminum silicate, polyvinyl alcohol and corn starch; E. dispersing agent: NN 0.
When the bactericide is an aqueous emulsion, the bactericide can contain one or more than two of the following auxiliary agents: A. emulsifier: nonylphenol polyoxyethylene (EO 10) ether phosphate, tristyrylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkylphenol ethoxylates, Nongru No. 700 (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Nongru No. 2201, span-60 (common name: sorbitan stearate), Tween-60 (common name: polyoxyethylene sorbitan stearate), TX-10 (common name: octylphenol polyoxyethylene (10) ether), Nongru 1601 (common name: tristyrylphenol polyoxypropylene polyoxyethylene block polymer), Nongru No. 600 and Nongru No. 400; B. solvent: one or more of dimethylbenzene, methylbenzene, cyclohexanone and solvent oil; C. a stabilizer: one or more of triphenyl phosphite, epichlorohydrin and epoxidized soybean oil; D. an antifreezing agent: one or more of ethylene glycol, propylene glycol, glycerol, urea, and inorganic salts such as sodium chloride; E. defoaming agent: one or more selected from silicone oil, silicone compound, C10-20 saturated fatty acid compound, and C8-10 fatty alcohol. F. Thickening agent: one or more of xanthan gum, polyvinyl alcohol, bentonite and magnesium aluminum silicate; G. preservative: one or more of formaldehyde, benzoic acid and sodium benzoate, and water is deionized water.
When the bactericide is a microemulsion, one or more than two of the following auxiliary agents can be contained: A. emulsifier: calcium dodecylbenzene sulfonate and any one or more of fatty acid polyoxyethylene ether, alkylphenol polyoxyethylene sulfosuccinate, styrylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, fatty acid polyoxyethylene ester and polyoxyethylene fatty alcohol ether; B. an antifreezing agent: one or more of ethylene glycol, propylene glycol and glycerol; C. a stabilizer: one of sodium citrate and resorcinol; D. solvent: xylene or biodiesel, toluene, diesel oil, methanol, ethanol, n-butanol, isopropanol, rosin-based vegetable oil with a code number of ND-45, solvent oil, dimethylformamide, dimethyl sulfoxide, water, and the like, wherein the water is deionized water; E. an antifreezing agent: one or more of ethylene glycol, propylene glycol, glycerol, urea, and inorganic salts such as sodium chloride; F. defoaming agent: one or more selected from silicone oil, silicone compound, C10-20 saturated fatty acid compound, and C8-10 fatty alcohol; G. thickening agent: one or more of xanthan gum, carboxymethyl cellulose, carboxyethyl cellulose, methyl cellulose, magnesium aluminum silicate and polyvinyl alcohol.
Specifically, the bactericide is water dispersible granules comprising 6-35% of iminoctadine, 1.8-54% of component II, 1-5% of alkylphenol polyoxyethylene ether, 1-3% of alkyl naphthalene sodium sulfonate, 5-10% of lignosulfonate, 1-3% of sodium dodecyl sulfate, 1-5% of sodium dodecyl benzene sulfonate, 5-10% of NN0, 10-15% of urea, 1-3% of magnesium chloride, 1-5% of light calcium carbonate, 1-3% of starch, corn starch or ammonium sulfate, and the balance is up to 100%.
Specifically, the bactericide is an aqueous emulsion and comprises 5-10% of trifloxystrobin, 1-15% of the component II, 1-5% of phenethyl phenol polyoxyethylene ether phosphate, 1-3% of alkylphenol polyoxyethylene ether, 1-3% of agricultural emulsifier 33#, 10-15% of solvent oil and water for supplementing to 100%.
Specifically, the bactericide is a microemulsion which comprises 5-30% of trifloxystrobin, 5-20% of the component II, 1-10% of calcium dodecyl benzene sulfonate, 1-5% of sodium dodecyl benzene sulfonate, 1-5% of phenethyl phenol polyoxyethylene ether phosphate, 1-3% of alkylphenol polyoxyethylene ether, 1-3% of agricultural emulsion No. 33, 10-15% of solvent oil, 10% of propylene glycol or 10% of isopropanol, and water for supplementing to 100%.
The pure product of the biguanide trioctyl benzene sulfonate is brown solid. The action mode is to inhibit the biosynthesis of germ lipoid, is a broad-spectrum fungicide, has strong local permeability and has high growth inhibition activity on certain pathogenic fungi.
The Xinjunan acetate is a quaternary ammonium salt bactericide, and the pure product is a colorless aqueous liquid. The sterilization is achieved by destroying cell membranes of various pathogens, coagulating protein, preventing respiration and enzyme activity and the like, has the functions of internal absorption and penetration, and has obvious killing and inhibiting effects on various plant fungi, bacteria and viruses causing crop diseases.
The phenazine alpha-2 carboxylic acid pure product is white or yellowish powder, and is effective to various crop diseases, especially powdery mildew, by combining a nanometer DV technology and a targeted pharmaceutical technology.
Prothioconazole is an inhibitor of sterol Demethylation (DMI) or ergosterol biosynthesis and is pure in the form of white or light grey brown powdery crystals. Has good systemic action, excellent protection, treatment and eradication activity and long lasting period.
The tioxim-methyl is a novel medicament for treating powdery mildew with special effect, and the pure product is colorless or yellowish oily liquid. The bactericidal composition is a new product newly developed on the basis of trifloxystrobin, adopts a development method combining a nano DV technology and a targeted pharmaceutical technology, improves the bactericidal strength by 5 times and 10 times compared with the trifloxystrobin, and has an obvious use effect in areas where powdery mildew generates resistance to the trifloxystrobin, the ether phenolsulfonate, the kresoxim-methyl, the polyoxin, the myclobutanil and the like.
The invention also comprises the application of the bactericidal composition or the bactericide in preventing and treating crop diseases,
the diseases comprise fungoid diseases such as scab, powdery mildew, black spot, brown spot and the like.
The crops comprise pear, apple, grape, peach tree, plum, apricot, cherry and orange.
The starting materials used in the present invention are commercially available or may be prepared by methods conventional in the art.
On the basis of the common knowledge in the field, the above preferred conditions can be combined with each other to obtain the preferred embodiments of the invention.
Further, it is to be understood that terms such as "comprising," "including," "containing," and the like, also include "consisting of … …," "consisting of … …," "made of … …," and the like, herein.
The agricultural bactericidal composition provided by the invention has high biological activity, small dosage in unit area and low application cost, and simultaneously, the biguanide trioctyl benzene sulfonate, the Xinjunan acetate, the phenazine alpha-2 carboxylic acid, the prothioconazole or the trifloxystrobin in the composition have low toxicity and the like, so the composition has small harm to people and livestock, has no pollution to the ecological environment, and is beneficial to biology and environmental safety. Furthermore, on the basis of synergistic interaction of the bactericidal composition, the generation and development of drug resistance of pathogenic bacteria can be delayed, the risk of resistance of pathogenic bacteria to a single bactericide is reduced, and the service life of each component in the bactericidal composition is prolonged. Furthermore, the bactericidal composition has very high control effect on pear scab, black spot, brown spot and powdery mildew fungal diseases, and improves the yield and quality of pears.
Detailed Description
The adjuvants used in the composition of the present invention include dispersing agents, emulsifying agents, dispersing agents, anti-freezing agents, thickening agents, wetting agents, etc. and other known substances useful for stabilizing the active ingredients in the formulation and exerting the medicinal effects, and are various ingredients commonly used or allowed to be used in the pesticide formulation, and are not particularly limited, and the specific ingredients and amounts thereof are determined by simple experiments according to the formulation requirements.
The following examples merely illustrate the formulation of some of the compositions, but are not intended to be limiting.
Example 1
Weighing 30% of iminoctadine tris, 1.8% of octopamine acetate, 4% of alkylphenol polyoxyethylene ether, 10% of NN0, 15% of urea and corn starch to 100%, fully mixing and crushing the components, boiling, drying and granulating to prepare the 31.8% iminoctadine acetate water dispersible granule.
Example 2
Weighing 6% of iminoctadine tris, 54% of phenazine alpha-2 carboxylic acid, 2% of sodium alkylnaphthalenesulfonate, 1.5% of sodium lignosulfonate, 2% of sodium dodecyl sulfate and ammonium sulfate to complement to 100%. The active ingredient, the dispersant, the wetting agent and the disintegrant are uniformly mixed according to the proportion of the formula, and are crushed into wettable powder by airflow, a certain amount of water is added for mixing and extruding to prepare materials, and the 60 percent biguanide trioctyl benzene sulfonate-phenazine alpha-2 carboxylic acid water dispersible granules are obtained after drying and screening.
Example 3
Weighing 35% of iminoctadine tris, 45% of prothioconazole, 6% of lignosulfonate, 4% of sodium dodecyl benzene sulfonate, 3% of magnesium chloride, 2% of starch and 5% of light calcium carbonate, and mixing to prepare the 80% iminoctadine, prothioconazole water dispersible granule.
Example 4
Weighing 5% of iminoctadine tris, 15% of thiotrifloxystrobin, 3% of phenethyl phenol polyoxyethylene ether phosphate, 2% of alkylphenol polyoxyethylene ether, 13% of solvent oil and 5% of glycol, and adding water to 100%. The biguanide trioctylphenyl sulfonate-thiotrifloxystrobin emulsion in water with the effective component content of 20 percent is prepared according to the conventional preparation method.
Example 5
Weighing 5% of iminoctadine tris, 1% of octopamine acetate, 3% of phenethyl phenol polyoxyethylene ether phosphate, 2% of farm milk No. 33, 1% of alkylphenol polyoxyethylene ether and 13% of solvent oil, and adding water to 100%. The raw materials are put into a mixing kettle according to a conventional method for preparing the emulsion in water and are mixed at high speed to prepare the emulsion in water of the iminoctadine tris (iminoctadine) acetate with the effective component content of 6 percent.
Example 6
Weighing 10% of iminoctadine tris, 5% of prothioconazole, 3% of phenethyl phenol polyoxyethylene ether phosphate, 2% of microemulsion No. 33, 1% of alkylphenol polyoxyethylene ether and 13% of solvent oil, and adding water to 100%. The raw materials are put into a mixing kettle according to a conventional method for preparing the aqueous emulsion and are mixed at high speed to prepare the biguanide trioctylphenyl sulfonate-prothioconazole aqueous emulsion with the main effective component content of 15 percent.
Example 7
Weighing 5% of iminoctadine tris, 5% of phenazine alpha-2 carboxylic acid, 6% of calcium dodecylbenzene sulfonate, 3% of sodium dodecylbenzene sulfonate, 1% of alkylphenol polyoxyethylene ether, 15% of solvent oil and 10% of propylene glycol, and adding water to 100%. The biguanide trioctylphenyl sulfonate-phenazine alpha-2 carboxylic acid microemulsion with the effective component content of 10 percent is prepared according to the conventional preparation method.
Example 8
Weighing 10% of iminoctadine tris, 20% of prothioconazole, 2% of calcium dodecylbenzenesulfonate, 3% of sodium dodecylbenzenesulfonate, 1% of alkylphenol polyoxyethylene, 15% of solvent oil and 10% of propylene glycol, and adding water to 100%. The biguanide trioctyl benzene sulfonate-prothioconazole microemulsion with the effective component content of 30 percent is prepared according to the conventional preparation method.
Example 9
Weighing 30% of biguanide trioctyl benzene sulfonate, 5% of tioximino-methyl, 6% of calcium dodecyl benzene sulfonate, 3% of phenethyl phenol polyoxyethylene ether phosphate, 2% of Nongru 33#, 1% of alkylphenol polyoxyethylene ether and 10% of isopropanol, and adding water to 100%. The raw materials are put into a mixing kettle to be mixed according to a conventional method for preparing the microemulsion, and the biguanide trioctylphenyl sulfonate-thiotrifloxystrobin microemulsion with the effective component content of 40 percent is prepared.
Example 10
Synergistic study of antibacterial composition
The synergistic research of mutual compounding is carried out by using iminoctadine tris (component one) and xinjunan acetate, phenazine alpha-2 carboxylic acid, prothioconazole or trifloxystrobin (component two), and the specific method comprises the following steps: firstly, determining the first component and the second componentEC of two single compounds (hereinafter referred to as agents)50Value (effective inhibitory median concentration of drug against pathogen) based on EC of two single agents in combination50The combination proportion of the medicaments is set, the R value of the synergistic coefficient is calculated according to the Wadley formula, and the proper combination proportion is determined.
The test target pathogenic bacteria are pear scab, powdery mildew, alternaria alternata and brown spot pathogen, but are not limited to the pear scab, powdery mildew, black spot pathogen and brown spot pathogen. The test method refers to agricultural industry standard NY/T1156.2-2006 of the people's republic of China, and adopts a defoliation method and a hypha growth rate method for measurement.
Respectively suspending the conidia of pear scab and pear powdery mildew at 1 × 105～106Spraying the mixture per ml onto pear tree leaf (ready for use), spraying test agents with different concentrations after 24h, culturing in constant temperature incubator at 18 deg.C and 28 deg.C for 7 days, and examining disease index.
Disease index ∑ (number of diseased leaves at each stage × number of relative stages)/(total number of investigated leaves × 9) × 100
Disease inhibition (%) (control disease index-treatment disease index)/control disease index x 100
Inoculating pear black spot and mycosphaerella petioli into the center of a PDA (personal digital Assistant) plate, culturing at 25 ℃ for 3 days, and punching holes at the edge close to a bacterial colony by using a sterile puncher to prepare a bacterial cake (the diameter phi of the bacterial cake is 5 mm). The cake was inoculated face down into the center of a plate of PDA medium containing a series of concentrations of test agent, 1 cake was inoculated per dish, and repeated 4 times at each treatment concentration. After culturing for 3 days in a constant temperature incubator at 25 ℃, the colony growth diameter was measured by the cross method, and the average growth inhibition (%) was calculated according to the following formula. The obtained data is processed by Finney probability analysis method, and the toxicity regression equation and EC are obtained by DPS statistical software50The values and correlation coefficients, the results are shown in tables 1 to 16.
Hypha growth inhibition (%) (control colony diameter-treated colony diameter)/control colony diameter × 100
The synergy coefficient (SR) was calculated according to the Wadley method. The combined virulence effect of the combination was evaluated by comparing the synergy coefficient (SR).
EC50(theory) ═ a + b)/(a/EC50A+b/EC50B)
SR＝EC50(theory)/EC50(practice)
A. B respectively represents two medicament components; a. b is the mass ratio of A, B components in the compounded combination.
When SR is less than or equal to 0.5, it is antagonistic; when SR is 0.5-1.5, it is additive action; when SR is more than or equal to 1.5, it is synergistic effect.
TABLE 1 results of Wadley assay of iminoctadine hydrochloride and xinjunan acetate composition on Venturia pear
TABLE 2 Wadley method for determining the results of the iminoctadine tris and octanediam acetate combinations on powdery mildew
TABLE 3 results of Wadley assay of iminoctadine tris and xinjunan acetate combinations on Erysiphe piricola
TABLE 4 results of Wadley assay of iminoctadine tris and xinjunan acetate combinations on Fusarium moniliforme
TABLE 5 Wadley assay of biguanide trioctylphenyl sulfonate and phenazine alpha-2 carboxylic acid compositions against Venturia pear
TABLE 6 Wadley assay results for the biguanide trioctylphenyl sulfonate and phenazine alpha-2 carboxylic acid compositions against Erysiphe cichoracearum
TABLE 7 Wadley assay results for the Respiromyces Pyricularis with biguanide trioctylphenyl sulfonate and phenazine alpha-2 carboxylic acid compositions
TABLE 8 Wadley assay of Trichostatin besylate and phenazine alpha-2 Carboxylic acid combinations for Pyricularia oryzae
TABLE 9 Wadley assay of biguanide trioctylphenyl sulfonate and prothioconazole combinations against venturia piricola
TABLE 10 Wadley assay results for erysiphe necator for biguanide trioctylphenyl sulfonate and prothioconazole compositions
TABLE 11 results of Wadley assay of combinations of iminoctadine tris and prothioconazole on Erysiphe pyrifera
TABLE 12 results of Wadley assay of combinations of iminoctadine tris and prothioconazole on Fusarium moniliforme
TABLE 13 Wadley assay results of biguanide trioctylphenyl sulfonate and thifluxastrobin compositions against Venturia pear
TABLE 14 Wadley method for determining the results of the biguanide trioctylphenyl sulfonate and the tioximostrobin composition on Erysiphe cichoracearum
TABLE 15 Wadley method determination of biguanide trioctylphenyl sulfonate and pyraclostrobin compositions on Pityrosporum ovale
TABLE 16 results of Wadley assay of combinations of iminoctadine tris and thienaxastrobin against Limonilinia
The determination result shows that the biguanide trioctyl benzene sulfonate (component one) and the Xinjunan acetate, the phenazine alpha-2 carboxylic acid, the prothioconazole or the trifloxystrobin (component two) are mixed according to the weight ratio of 1: 10-10: 1, so that the composition has high toxicity on pear scab, powdery mildew, black spot and brown spot, and the mixture of the biguanide trioctyl benzene sulfonate and the Xinjunan acetate has a remarkable synergistic effect.
The agents used in the following examples were prepared in accordance with examples 1-9.
Example 11
Test of control effect of bactericidal composition on pear scab
The test is carried out according to the 'pesticide field efficacy test criterion of pesticide' GBT17980.24-2000 'field efficacy test criterion of bactericide for preventing and treating pear scab' of ministry of agriculture.
The test field is located in Xinji small Chencun in North of Xinji city in Hebei province, the first pesticide is sprayed 10 days after the pear trees wither flowers, the pesticide liquid amount per mu is 250KG, the pesticide is continuously applied for 4 times, the pesticide application interval is 10-15 days, the field disease condition is investigated 14 days after the last pesticide application, and the disease index and the prevention and treatment effect are calculated.
The drug effect calculation method comprises the following steps:
disease index ∑ (disease leaf number at each stage × relative stage value)/(total leaf number for investigation × 9) × 100 control effect (%) (disease index in control blank area-disease index in control area)/disease index in control blank area × 100 table 17 iminoctadine tricaprylylphonate (component one), xinjunan acetate, phenazine alpha-2 carboxylic acid, prothioconazole or thiflustrobin (component two) bactericidal composition for controlling pear scab
Note: the letters in the column "significance of difference" represent the difference in significance of duncan repolarization for analysis.
The result shows that the bactericidal composition containing the iminoctadine tris (iminoctadine) and the xinafoate acetate, the phenazine alpha-2 carboxylic acid, the prothioconazole or the trifloxystrobin (component two) has good control effect on the pear scab, can achieve higher control effect under the same dosage or lower dosage, and has control effect higher than 85% under each treatment dosage and obviously higher than that of a control medicament.
Example 12
Test of control effect of bactericidal composition on pear powdery mildew
The test is carried out according to the pesticide inspection institute of Ministry of agriculture, pesticide field efficacy test criteria GB/T17980.10-2000' pesticide field efficacy test criteria for preventing and treating pear powdery mildew by bactericide
The test field is located in the mounow village of Potou city in Hebei province, the first time of pesticide spraying is started when powdery mildew of pears occurs, the pesticide liquid amount per mu is 250KG, the pesticide application is continuously carried out for 3 times, the pesticide application interval is 30 days, the field morbidity is investigated 20 days after the last pesticide application, and the disease index and the prevention and treatment effect are calculated.
The drug effect calculation method comprises the following steps:
disease index ∑ (number of diseased leaves at each stage × number of relative stages)/(total number of investigated leaves × 9) × 100
Preventing and treating effect (%) - (blank control area disease index-preventing and treating area disease index)/blank control area disease index is multiplied by 100
TABLE 18 field efficacy of iminoctadine tris (component one) and xinjunan acetate, phenazine alpha-2 carboxylic acid, prothioconazole or trifloxystrobin (component two) fungicidal compositions for the control of pear powdery mildew
Note: the letters in the column "significance of difference" represent the difference in significance of duncan repolarization for analysis.
The result shows that the bactericidal composition containing the iminoctadine tris (octyl benzene sulfonate) (component one) and the Xinjunan acetate, the phenazine alpha-2 carboxylic acid, the prothioconazole or the trifloxystrobin (component two) has good control effect on the powdery mildew of pears, can achieve higher control effect under the same dosage or lower dosage, and the control effect of each treatment dosage of the bactericidal composition is higher than 85 percent and is obviously higher than that of a control medicament.
Example 13
Test of control effect of bactericidal composition on pear black spot
The test is carried out according to the 'pesticide field efficacy test criterion of pesticide' GB/T1798044-2000 'field efficacy test criterion of bactericide for preventing and treating pear black spot disease' of ministry of agriculture.
The test field is located in Zhao county of Hebei province, the first time of application is started when the black spot disease of the pear occurs sporadically, the amount of the application liquid is 250KG per mu, the application is continuously carried out for 3 times, the application interval is 10-15 days, the field incidence condition is investigated 14 days after the last application, and the disease index and the prevention and treatment effect are calculated.
The drug effect calculation method comprises the following steps:
disease index ∑ (number of diseased leaves at each stage × number of relative stages)/(total number of investigated leaves × 9) × 100
Preventing and treating effect (%) - (blank control area disease index-preventing and treating area disease index)/blank control area disease index is multiplied by 100
TABLE 19 field efficacy of biguanide trioctyl benzene sulfonate (component one) and xinjunan acetate, phenazine alpha-2 carboxylic acid, prothioconazole or trifloxystrobin (component two) bactericidal composition for preventing and treating pear black spot
Note: the letters in the column "significance of difference" represent the difference in significance of duncan repolarization for analysis.
The result shows that the bactericidal composition containing the iminoctadine tris (component I) and the xinjunan acetate, the phenazine alpha-2 carboxylic acid, the prothioconazole or the trifloxystrobin (component II) has good control effect on the pear black spot, and the control effect under each treatment dosage is higher than 82%, which is obviously higher than that of a control medicament.
Example 14
Test of control effect of bactericidal composition on cercospora brown spot
The test is carried out according to the pesticide inspection institute of Ministry of agriculture, pesticide field efficacy test criterion GB/T17980.10-2000' field efficacy test criterion for preventing and treating cercospora brown spot of bactericide
The test field is located in the great hutun of Potou city in Hebei province, the first pesticide is sprayed when the cercospora leaf spot occurs sporadically, the pesticide liquid amount per mu is 250KG, the pesticide is continuously applied for 3 times at a pesticide application interval of 14-25 days, the pesticide application time is determined according to the disease condition and climatic conditions, the field disease condition is investigated 14 days after the last pesticide application, and the disease index and the prevention and treatment effect are calculated.
The drug effect calculation method comprises the following steps:
disease index ∑ (number of diseased leaves at each stage × number of relative stages)/(total number of investigated leaves × 9) × 100
Preventing and treating effect (%) - (blank control area disease index-preventing and treating area disease index)/blank control area disease index is multiplied by 100
TABLE 20 field efficacy of the biguanide trioctylphenyl sulfonate (component one) and xinjunan acetate, phenazine alpha-2 carboxylic acid, prothioconazole or trifloxystrobin (component two) bactericidal composition for preventing and treating cercospora leaf spot
Note: the letters in the column "significance of difference" represent the difference in significance of duncan repolarization for analysis.
The result shows that the bactericidal composition containing the iminoctadine tris (iminoctadine) and the xinafoate acetate, the phenazine alpha-2 carboxylic acid, the prothioconazole or the trifloxystrobin (component two) has good control effect on the cercospora brown spot, can achieve higher control effect under the same dosage or lower dosage, and has control effect higher than 85% under each treatment dosage and is obviously higher than that of a control medicament.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (13)
1. A germicidal composition, characterized by active ingredients comprising: a first component and a second component; the component one is biguanide trioctyl benzene sulfonate; the second component is prothioconazole; wherein the mass ratio of the first component to the second component is 1: 20-20: 1.
2. The bactericidal composition according to claim 1, wherein the mass ratio of the first component to the second component is 1:15 to 15: 1.
3. The bactericidal composition according to claim 1, wherein the mass ratio of the first component to the second component is 1:10 to 10: 1.
4. The bactericidal composition according to claim 1, wherein the mass ratio of the first component to the second component is 1: 8-8: 1.
5. The bactericidal composition according to claim 1, wherein the mass ratio of the first component to the second component is 1:5 to 5: 1.
6. The bactericidal composition of claim 1, wherein the mass ratio of the first component to the second component is 1: 1.
7. The germicidal composition of any of claims 1-6, wherein the active ingredient is comprised of the first component and the second component.
8. A bactericide which is prepared from the bactericidal composition of any one of claims 1 to 6 and an auxiliary agent or carrier usable in the field of agricultural chemicals.
9. The fungicide according to claim 8, which is a water dispersible granule.
10. The bactericide as claimed in claim 8, wherein the total mass of the first component and the second component accounts for 5-80% of the total mass of the bactericide.
11. The bactericide as claimed in claim 8, wherein the total mass of the first component and the second component accounts for 20-60% of the total mass of the bactericide.
12. The bactericide as claimed in claim 8, which is water dispersible granule comprising: 35% of iminoctadine tris, 45% of prothioconazole, 6% of lignosulfonate, 4% of sodium dodecyl benzene sulfonate, 3% of magnesium chloride, 2% of starch and 5% of light calcium carbonate.
13. Use of the fungicidal composition according to any one of claims 1 to 7 or the fungicidal composition according to any one of claims 8 to 12 for the control of crop diseases; the diseases are scab, powdery mildew, black spot and brown spot; the crops comprise pears, apples, grapes, peach trees, plums, apricots, cherries and oranges.
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