CN111407685A - Anti-aging composition of recombinant fibronectin-human-like collagen and preparation method thereof - Google Patents
Anti-aging composition of recombinant fibronectin-human-like collagen and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of protein, in particular to a recombinant fibronectin-human-like collagen anti-aging composition and a preparation method thereof. The composition comprises the following components in parts by weight: 1-5 parts of a first emulsifier, 0.1-1 part of a second emulsifier, 1-3 parts of cetostearyl alcohol, 0.5-2 parts of shea butter, 1-5 parts of jojoba seed oil, 1-5 parts of hydrogenated polyisobutene, 1-5 parts of isononyl isononanoate, 1-5 parts of Acticire, 0.1-1 part of phytosterol/octyldodecanol lauroyl glutamate, 0.1-1 part of tocopherol acetate and 0.1-0.5 part of recombinant fibronectin-human-like collagen concentrated solution. The invention takes the recombinant fibronectin-human-like collagen raw material with high safety and good anti-aging effect as a main efficacy agent to assist various micromolecular peptides and smooth fine wrinkles. Meanwhile, the moisture-keeping functional components of the polyglutamic acid sodium and the glucan can fully supplement water and water for the skin on the basis of anti-aging, so that the skin is glossy, fine and elastic.
Description
Technical Field
The invention relates to the technical field of protein, in particular to a recombinant fibronectin-human-like collagen anti-aging composition and a preparation method thereof.
Background
Fibronectin (FN) is a multifunctional protein existing in human tissues and cells, is a high-molecular and high-activity glycoprotein, is a protein dimer, is composed of two similar subunits, is formed by crosslinking through a C-terminal disulfide bond, and has a V-shaped whole molecule and a molecular weight of 440-450 KDa. The molecular weight of each subunit is 220-250 kDa. Fibronectin in human blood, cell surface, clinically demonstrated: fibronectin stimulates skin cells to produce collagen, thereby restoring skin elasticity. Comprehensively regenerating cells of each layer of skin: not only can resist aging, but also can comprehensively repair red blood streak and allergic skin. Comprehensively repairing cells of each layer of skin: phagocytizing and cleaning pathogenic bacteria and harmful components in cell living environment, and allowing skin to generate various moisturizing nutritional factors (hyaluronic acid and ceramide), thereby regulating moisture from inside to outside to moisten skin.
Collagen is a biological high molecular substance, and is a white, opaque, unbranched fibrous protein. The collagen is the main component in human skin, accounts for 70% -80% of extracellular matrix, forms a fine elastic net in the skin, locks moisture, supports the skin like a bracket, and enables the skin to be full of elasticity, moist, fine and smooth. The collagen industry has been in history for decades abroad, and based on developed economic foundations, advanced biotechnology, mature industry environment and green and healthy consumption concept, the collagen market in Europe and America is relatively mature, and collagen foods, health care products and cosmetics become products favored by people in Europe and America, Japan and other developed countries and are widely applied.
Fibronectin has wide application and huge market in the fields of medicine, cosmetics and scientific research, but the yield of natural and high-purity fibronectin extracted from human or animal blood and tissues is extremely limited and the cost is high. Limiting the use and production of FN in many ways.
The traditional collagen is mainly extracted from animal connective tissues, but has risks of animal-derived diseases and the like, and the prepared collagen product has weaker tensile strength, faster degradation of pure collagen in vivo and possible potential antigenicity and the like, and simultaneously, due to the difference of the collagen source, the processing technology and the raw material proportion, the nutritional ingredients and the feeding value of the product are different, and the leather is not only contacted with a plurality of chemical substances in the processing process, but also is easily infected by bacteria, thereby greatly limiting the potential application and development of the leather.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: in order to solve the technical problems described in the background technology, the invention provides a recombinant fibronectin-human-like collagen anti-aging composition and a preparation method thereof. Meanwhile, moisturizing functional components such as sodium polyglutamate and glucan can fully moisturize and moisturize the skin on the basis of resisting aging, so that the skin is glossy, fine and elastic.
A recombinant fibronectin-human-like collagen anti-aging composition comprises the following raw materials in parts by weight:
1-5 parts of a first emulsifier, 0.1-1 part of a second emulsifier, 1-3 parts of cetostearyl alcohol, 0.5-2 parts of shea butter, 1-5 parts of jojoba seed oil, 1-5 parts of hydrogenated polyisobutene, 1-5 parts of isononyl isononanoate, 1-5 parts of acticore, 0.1-1 part of phytosterol/octyldodecanol lauroyl glutamate, 0.1-1 part of tocopherol acetate, 0.1-1 part of glyceramide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1-5 parts of polydimethylsiloxane, 1-5 parts of DC-3451-5 parts of water, 100 parts of 1, 3-propylene glycol, 1-10 parts of glycerol, 0.1-2 parts of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.1-1 part of lactobacillus/rice fermentation product, 0.1-0.5 part of allantoin, 0.01-0.2 part of β -dextran, 1-5 parts of trehalose, 0.01-5 parts of polyglutamic acid yeast, 0.01-2 parts of hyaluronic acid, 0.01-352 parts of a recombinant human collagen peptide, 0.1-2 parts of matrine-2 parts of sodium hyaluronate, 0.01-2 parts of a biological hydrolysate and 0.1-1-2 parts of a recombinant polypeptide.
Specifically, 3-5 parts of first emulsifier, 0.5-1 part of second emulsifier, 2-3 parts of cetearyl alcohol, 1-2 parts of shea butter, 2-5 parts of jojoba seed oil, 2-5 parts of hydrogenated polyisobutene, 3-5 parts of isononyl isononanoate, 2-5 parts of Acticire, 0.5-1 part of phytosterol/octyldodecanol lauroyl glutamate, 0.5-1 part of tocopherol acetate, 0.5-1 part of glyceramide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1.5-5 parts of polydimethylsiloxane, 1.5-5 parts of DC-3452-5 parts of water, 100 parts of 1, 3-propylene glycol 3-10 parts of glycerol 3-10 parts of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.8-2 parts of lactobacillus/rice fermentation product, 0.5-1 part of allantoin, 0.15-0.5 part of β -0.05-0.2 part of dextran, 0.5-2 parts of trehalose, 0.8-2 parts of trehalose, 0.5-2 parts of hyaluronic acid, 0.5-2 parts of dextran sulfate, 0.5-2.5-2 parts of collagen hydrolysate, 0.05-2 parts of collagen hydrolysate, 0.5-2 parts of collagen hydrolysate and 0.11 parts of collagen hydrolysate.
Specifically, the percentage of each component of the emulsifier is as follows: candelilla wax/jojoba/rice bran polyglycerol-3 esters 30%, glyceryl stearate 30%, cetostearyl alcohol 20%, sodium stearoyl lactylate 20%.
Specifically, the percentage of each component of the emulsifier II is as follows: 80% of C14-22 alcohol and 20% of C12-20 alkyl glucoside.
Specifically, the percentage of each component of Acticire is as follows: 60% of jojoba esters, 4% of cercis chinensis flower wax, 32% of sunflower seed wax and 34% of polyglycerol-34%.
Specifically, the GC-04 comprises the following components in percentage: 2% of glyceryl caprylate, 13% of caprylyl hydroximic acid, 1% of p-hydroxyacetophenone, 81% of propylene glycol and 3% of water.
Specifically, the Matrixyl3000 comprises the following components in percentage by weight: 53.485% of glycerin, 25% of water, 20% of butanediol, 1% of carbomer, 200.5% of polysorbate, 10.01% of palmitoyl tripeptide and 70.005% of palmitoyl tetrapeptide.
Specifically, the percentage of each component of the recombinant fibronectin-human-like collagen concentrated solution is as follows: 50% of fibronectin and 50% of human-like collagen.
A preparation method of a recombinant fibronectin-human-like collagen anti-aging composition comprises the following steps:
a) adding emulsifier I, emulsifier II, cetostearyl alcohol, shea butter, jojoba seed oil, hydrogenated polyisobutene, isononyl isononanoate, Acticire, phytosterol/octyldodecanol lauroyl glutamate, tocopherol acetate, glyceramide ethanol methacrylate/stearyl alcohol methacrylate copolymer, polydimethylsiloxane and DC-345 into an oil phase pot at 80-86 ℃ according to a ratio, uniformly stirring, and keeping the temperature for 10 minutes;
b) adding water, 1, 3-propylene glycol, glycerol, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, lactobacillus/rice fermentation product, allantoin, β -glucan, trehalose, sodium polyglutamate and sodium hyaluronate into an emulsifying pot according to a ratio, uniformly stirring, heating the emulsifying pot to 85-95 ℃, and then preserving heat and stirring for 30 minutes;
c) pumping the materials in the oil phase pot into an emulsifying pot, stirring and homogenizing for 5 minutes, keeping the temperature and stirring for 20 minutes continuously, and then cooling to 42 ℃;
d) then putting GC-04, carnosine, the lysate of the yeast fermentation product of the second split yeast, Matrixyl3000, bioglycan-1, acetyl tetrapeptide-2 and the recombinant fibronectin-human-like collagen concentrated solution into an emulsifying pot according to the proportion, stirring for 20 minutes, and cooling to 38 ℃;
e) and finally filtering and discharging.
The invention has the beneficial effects that: the invention provides a recombinant fibronectin-human-like collagen anti-aging composition and a preparation method thereof, wherein a recombinant fibronectin-human-like collagen raw material with high safety and good anti-aging effect is used as a main efficacy agent to assist various small molecular peptides and smooth fine wrinkles. Meanwhile, moisturizing functional components such as sodium polyglutamate and glucan can fully moisturize and moisturize the skin on the basis of resisting aging, so that the skin is glossy, fine and elastic.
Detailed Description
The present invention will now be described in further detail.
A recombinant fibronectin-human-like collagen anti-aging composition comprises the following raw materials in parts by weight:
1-5 parts of a first emulsifier, 0.1-1 part of a second emulsifier, 1-3 parts of cetostearyl alcohol, 0.5-2 parts of shea butter, 1-5 parts of jojoba seed oil, 1-5 parts of hydrogenated polyisobutene, 1-5 parts of isononyl isononanoate, 1-5 parts of acticore, 0.1-1 part of phytosterol/octyldodecanol lauroyl glutamate, 0.1-1 part of tocopherol acetate, 0.1-1 part of glyceramide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1-5 parts of polydimethylsiloxane, 1-5 parts of DC-3451-5 parts of water, 100 parts of 1, 3-propylene glycol, 1-10 parts of glycerol, 0.1-2 parts of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.1-1 part of lactobacillus/rice fermentation product, 0.1-0.5 part of allantoin, 0.01-0.2 part of β -dextran, 1-5 parts of trehalose, 0.01-5 parts of polyglutamic acid yeast, 0.01-2 parts of hyaluronic acid, 0.01-352 parts of a recombinant human collagen peptide, 0.1-2 parts of matrine-2 parts of sodium hyaluronate, 0.01-2 parts of a biological hydrolysate and 0.1-1-2 parts of a recombinant polypeptide.
3-5 parts of a first emulsifier, 0.5-1 part of a second emulsifier, 2-3 parts of cetostearyl alcohol, 1-2 parts of shea butter, 2-5 parts of jojoba seed oil, 2-5 parts of hydrogenated polyisobutene, 3-5 parts of isononyl isononanoate, 2-5 parts of Acticire, 0.5-1 part of phytosterol/octyldodecanol lauroyl glutamate, 0.5-1 part of tocopherol acetate, 0.5-1 part of glyceramide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1.5-5 parts of polydimethylsiloxane, 0.5-5 parts of DC-3452-5 parts of water, 100 parts of 1, 3-propylene glycol, 3-10 parts of glycerol, 0.8-2 parts of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.5-1 part of lactobacillus/rice fermentation product, 0.15-0.5 part of allantoin, 0.05-0.2 part of β -dextran, 2-5 parts of trehalose, 0.01-2.5 parts of polyglutamic acid sodium hyaluronate, 0.5-2.5 parts of a lactic acid bacterium/rice fermentation product, 0.5-1.5 parts of a recombinant human collagen peptide, 0.05-6311 parts of sodium hyaluronate, 0.5-2 parts of a biological hydrolysate, 0.05-2 parts of a collagen-2 parts of a polypeptide, and 2 parts of a collagen hydrolysate.
The percentage of the emulsifier A is as follows: candelilla wax/jojoba/rice bran polyglycerol-3 esters 30%, glyceryl stearate 30%, cetostearyl alcohol 20%, sodium stearoyl lactylate 20%. The percentage of the emulsifier II is as follows: 80% of C14-22 alcohol and 20% of C12-20 alkyl glucoside. The percentage of each component of Acticire is as follows: 60% of jojoba esters, 4% of cercis chinensis flower wax, 32% of sunflower seed wax and 34% of polyglycerol-34%. The percentage of each component of GC-04 is as follows: 2% of glyceryl caprylate, 13% of caprylyl hydroximic acid, 1% of p-hydroxyacetophenone, 81% of propylene glycol and 3% of water. The percentage of each component of the Matrixyl3000 is as follows: 53.485% of glycerin, 25% of water, 20% of butanediol, 1% of carbomer, 200.5% of polysorbate, 10.01% of palmitoyl tripeptide and 70.005% of palmitoyl tetrapeptide. The percentage of each component of the recombinant fibronectin-human-like collagen concentrated solution is as follows: 50% of fibronectin and 50% of human-like collagen.
A preparation method of a recombinant fibronectin-human-like collagen anti-aging composition comprises the following steps:
a) adding emulsifier I, emulsifier II, cetostearyl alcohol, shea butter, jojoba seed oil, hydrogenated polyisobutene, isononyl isononanoate, Acticire, phytosterol/octyldodecanol lauroyl glutamate, tocopherol acetate, glyceramide ethanol methacrylate/stearyl alcohol methacrylate copolymer, polydimethylsiloxane and DC-345 into an oil phase pot at 80-86 ℃ according to a ratio, uniformly stirring, and keeping the temperature for 10 minutes;
b) adding water, 1, 3-propylene glycol, glycerol, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, lactobacillus/rice fermentation product, allantoin, β -glucan, trehalose, sodium polyglutamate and sodium hyaluronate into an emulsifying pot according to a ratio, uniformly stirring, heating the emulsifying pot to 85-95 ℃, and then preserving heat and stirring for 30 minutes;
c) pumping the materials in the oil phase pot into an emulsifying pot, stirring and homogenizing for 5 minutes, keeping the temperature and stirring for 20 minutes continuously, and then cooling to 42 ℃;
d) then putting GC-04, carnosine, the lysate of the yeast fermentation product of the second split yeast, Matrixyl3000, bioglycan-1, acetyl tetrapeptide-2 and the recombinant fibronectin-human-like collagen concentrated solution into an emulsifying pot according to the proportion, stirring for 20 minutes, and cooling to 38 ℃;
e) and finally filtering and discharging.
The first embodiment is as follows:
adding 3 parts of emulsifier I, 0.5 part of emulsifier II, 2 parts of cetostearyl alcohol, 1 part of shea butter, 2 parts of jojoba seed oil, 2 parts of hydrogenated polyisobutene, 3 parts of isononyl isononanoate, 2 parts of Acticire, 0.5 part of phytosterol/octyldodecanol lauroyl glutamate, 0.5 part of tocopherol acetate, 0.5 part of glycerylamide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1.5 parts of polydimethylsiloxane and DC-3452 parts into an oil phase pot at 85 ℃, uniformly stirring, and preserving heat for 10 minutes;
b) adding 100 parts of water, 3 parts of 1, 3-propylene glycol, 3 parts of glycerol, 0.8 part of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.5 part of lactobacillus/rice fermentation product, 0.15 part of allantoin, 0.05 part of β -glucan, 2 parts of trehalose, 0.1 part of sodium polyglutamate and 0.05 part of sodium hyaluronate into an emulsifying pot, stirring uniformly, raising the temperature of the emulsifying pot to 85 ℃, and then preserving heat and stirring for 30 minutes;
c) pumping the materials in the oil phase pot into an emulsifying pot, stirring and homogenizing for 5 minutes, keeping the temperature and stirring for 20 minutes continuously, and then cooling to 42 ℃;
d) then adding GC-040.8 parts, carnosine 0.15 part, schizosaccharomyces cerevisiae fermentation product lysate 2 parts, Matrixyl 30002 parts, bioglycan gum-12 parts, acetyl tetrapeptide-22 parts and recombinant fibronectin-human-like collagen concentrated solution 0.5 part into an emulsifying pot, stirring for 20 minutes, and cooling to 38 ℃;
e) and finally filtering and discharging.
Example two:
adding 3 parts of emulsifier I, 0.5 part of emulsifier II, 2 parts of cetostearyl alcohol, 1 part of shea butter, 2 parts of jojoba seed oil, 2 parts of hydrogenated polyisobutene, 3 parts of isononyl isononanoate, 2 parts of Acticire, 0.5 part of phytosterol/octyldodecanol lauroyl glutamate, 0.5 part of tocopherol acetate, 0.5 part of glycerylamide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1.5 parts of polydimethylsiloxane and DC-3452 parts into an oil phase pot at 85 ℃, uniformly stirring, and preserving heat for 10 minutes;
b) adding 100 parts of water, 3 parts of 1, 3-propylene glycol, 3 parts of glycerol, 0.8 part of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.5 part of lactobacillus/rice fermentation product, 0.15 part of allantoin, 0.05 part of β -glucan, 2 parts of trehalose, 0.1 part of sodium polyglutamate and 0.05 part of sodium hyaluronate into an emulsifying pot, stirring uniformly, raising the temperature of the emulsifying pot to 85 ℃, and then preserving heat and stirring for 30 minutes;
c) pumping the materials in the oil phase pot into an emulsifying pot, stirring and homogenizing for 5 minutes, keeping the temperature and stirring for 20 minutes continuously, and then cooling to 42 ℃;
d) then adding GC-040.8 parts, carnosine 0.15 part, schizosaccharomyces cerevisiae fermentation product lysate 2 parts, Matrixyl 30002 parts, bioglycan gum-12 parts, acetyl tetrapeptide-22 parts and recombinant fibronectin-human-like collagen concentrated solution 0.3 part into an emulsifying pot, stirring for 20 minutes and cooling to 38 ℃;
e) and finally filtering and discharging.
Example three:
adding 3 parts of emulsifier I, 0.5 part of emulsifier II, 2 parts of cetostearyl alcohol, 1 part of shea butter, 2 parts of jojoba seed oil, 2 parts of hydrogenated polyisobutene, 3 parts of isononyl isononanoate, 2 parts of Acticire, 0.5 part of phytosterol/octyldodecanol lauroyl glutamate, 0.5 part of tocopherol acetate, 0.5 part of glycerylamide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1.5 parts of polydimethylsiloxane and DC-3452 parts into an oil phase pot at 85 ℃, uniformly stirring, and preserving heat for 10 minutes;
b) adding 100 parts of water, 3 parts of 1, 3-propylene glycol, 3 parts of glycerol, 0.8 part of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.5 part of lactobacillus/rice fermentation product, 0.15 part of allantoin, 0.05 part of β -glucan, 2 parts of trehalose, 0.1 part of sodium polyglutamate and 0.05 part of sodium hyaluronate into an emulsifying pot, stirring uniformly, raising the temperature of the emulsifying pot to 85 ℃, and then preserving heat and stirring for 30 minutes;
c) pumping the materials in the oil phase pot into an emulsifying pot, stirring and homogenizing for 5 minutes, keeping the temperature and stirring for 20 minutes continuously, and then cooling to 42 ℃;
d) then adding GC-040.8 parts, carnosine 0.15 part, schizosaccharomyces cerevisiae fermentation product lysate 2 parts, Matrixyl 30002 parts, bioglycan gum-12 parts, acetyl tetrapeptide-22 parts and recombinant fibronectin-human-like collagen concentrated solution 0.1 part into an emulsifying pot, stirring for 20 minutes, and cooling to 38 ℃;
e) and finally filtering and discharging.
Comparative example one:
adding 3 parts of emulsifier I, 0.5 part of emulsifier II, 2 parts of cetostearyl alcohol, 1 part of shea butter, 2 parts of jojoba seed oil, 2 parts of hydrogenated polyisobutene, 3 parts of isononyl isononanoate, 2 parts of Acticire, 0.5 part of phytosterol/octyldodecanol lauroyl glutamate, 0.5 part of tocopherol acetate, 0.5 part of glycerylamide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1.5 parts of polydimethylsiloxane and DC-3452 parts into an oil phase pot at 85 ℃, uniformly stirring, and preserving heat for 10 minutes;
b) adding 100 parts of water, 3 parts of 1, 3-propylene glycol, 3 parts of glycerol, 0.8 part of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.5 part of lactobacillus/rice fermentation product, 0.15 part of allantoin, 0.05 part of β -glucan, 2 parts of trehalose, 0.1 part of sodium polyglutamate and 0.05 part of sodium hyaluronate into an emulsifying pot, stirring uniformly, raising the temperature of the emulsifying pot to 85 ℃, and then preserving heat and stirring for 30 minutes;
c) pumping the materials in the oil phase pot into an emulsifying pot, stirring and homogenizing for 5 minutes, keeping the temperature and stirring for 20 minutes continuously, and then cooling to 42 ℃;
d) then adding GC-040.8 parts, carnosine 0.3 part, schizosaccharomyces cerevisiae fermentation product lysate 4 parts, Matrixyl 30004 parts, bioglycan-12 parts and acetyl tetrapeptide-24 parts into an emulsifying pot, stirring for 20 minutes, and cooling to 38 ℃;
e) and finally filtering and discharging.
Comparative example two:
adding 3 parts of emulsifier I, 0.5 part of emulsifier II, 2 parts of cetostearyl alcohol, 1 part of shea butter, 2 parts of jojoba seed oil, 2 parts of hydrogenated polyisobutene, 3 parts of isononyl isononanoate, 2 parts of Acticire, 0.5 part of phytosterol/octyldodecanol lauroyl glutamate, 0.5 part of tocopherol acetate, 0.5 part of glycerylamide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1.5 parts of polydimethylsiloxane and DC-3452 parts into an oil phase pot at 85 ℃, uniformly stirring, and preserving heat for 10 minutes;
b) adding 100 parts of water, 3 parts of 1, 3-propylene glycol, 3 parts of glycerol, 0.8 part of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.5 part of lactobacillus/rice fermentation product, 0.15 part of allantoin, 0.05 part of β -glucan, 2 parts of trehalose, 0.1 part of sodium polyglutamate and 0.05 part of sodium hyaluronate into an emulsifying pot, stirring uniformly, raising the temperature of the emulsifying pot to 85 ℃, and then preserving heat and stirring for 30 minutes;
c) pumping the materials in the oil phase pot into an emulsifying pot, stirring and homogenizing for 5 minutes, keeping the temperature and stirring for 20 minutes continuously, and then cooling to 42 ℃;
d) then adding GC-040.8 parts, carnosine 0.3 part, schizosaccharomyces cerevisiae fermentation product lysate 4 parts, Matrixyl 30004 parts, bioglycan gum-12 parts, acetyl tetrapeptide-24 parts and recombinant fibronectin-human-like collagen concentrated solution 0.3 part into an emulsifying pot, stirring for 20 minutes and cooling to 38 ℃;
e) and finally filtering and discharging.
Comparative example three:
adding 3 parts of emulsifier I, 0.5 part of emulsifier II, 2 parts of cetostearyl alcohol, 1 part of shea butter, 2 parts of jojoba seed oil, 2 parts of hydrogenated polyisobutene, 3 parts of isononyl isononanoate, 2 parts of Acticire, 0.5 part of phytosterol/octyldodecanol lauroyl glutamate, 0.5 part of tocopherol acetate, 0.5 part of glycerylamide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1.5 parts of polydimethylsiloxane and DC-3452 parts into an oil phase pot at 85 ℃, uniformly stirring, and preserving heat for 10 minutes;
b) adding 100 parts of water, 3 parts of 1, 3-propylene glycol, 3 parts of glycerol, 0.8 part of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.5 part of lactobacillus/rice fermentation product, 0.15 part of allantoin, 0.05 part of β -glucan, 2 parts of trehalose, 0.1 part of sodium polyglutamate and 0.05 part of sodium hyaluronate into an emulsifying pot, stirring uniformly, raising the temperature of the emulsifying pot to 85 ℃, and then preserving heat and stirring for 30 minutes;
c) pumping the materials in the oil phase pot into an emulsifying pot, stirring and homogenizing for 5 minutes, keeping the temperature and stirring for 20 minutes continuously, and then cooling to 42 ℃;
d) then adding GC-040.8 parts, carnosine 0.3 part, schizosaccharomyces cerevisiae fermentation product lysate 4 parts, Matrixyl 30004 parts, bioglycan gum-12 parts, acetyl tetrapeptide-24 parts and recombinant fibronectin-human-like collagen concentrated solution 0.1 part into an emulsifying pot, stirring for 20 minutes and cooling to 38 ℃;
e) and finally filtering and discharging.
The formula product of the invention needs instruments in the preparation process:
| name of instrument | Model number | Brand |
| Homogenizer | T25 | German IKA |
| Mixer | RW20 | German IKA |
| Electronic scale | Shuangjie 1000g | Double Jie of ever-cooked |
| PH meter | FE28 | Mettler Switzerland&Tuoli duo |
| Constant temperature water bath kettle | HHS-11-1 | Shanghai Bo news |
| Water purifier | RO-1851 | Qin Yuan Zhejiang |
| Beaker | 500ml | Sichuan glass |
| Skin tester | MPA 9 | Germany CK |
| 3D facial image instrument | reveal imager | Canfield of America |
Skin elasticity test experiment
Testing an instrument: cotomermameter MPA580, manufactured by Courage + Khazaka, Germany (CK, Germany), the probe used was a Revisometer RV600 elastofiber tissue testing probe.
Subject: normal female subjects between 30-60 years of age.
Grouping: the subjects were divided into 6 groups of 20 persons each at each age, wherein the mean age was 35 years for the group of example 1, 58 years for the group of example 2, and 45 years for the group of example 3; the average age of the groups of comparative examples 1-3 was 45 years.
The tester area: the forehead area 2cm above the middle of the eyebrow, the chin area 2cm below the middle of the lower lip, and the left and right cheek areas at the intersection of the nose tip and the pupil.
The testing steps are as follows: the blank values were measured 30min after cleaning of the test area (R2, R5, R7, where R2 ═ Ua/Uf, R5 ═ Ur/Ue, R7 ═ Ur/Uf).
Uf — maximum amount of skin stretch;
ue-the amount of stretching of the skin at 0.1 second after a constant negative pressure is applied to the skin, the amount of stretching of the elastic portion is located;
ur-after negative pressure is eliminated, the skin can quickly recover to the original state, and the skin is also divided into an elastic part value Ur, namely, after the negative pressure is eliminated for 0.1 second, the recovery value and the viscoelastic part value, or called as a plastic part value, of the skin;
ua-the recovery value of the skin when negative pressure is added on the skin surface from the elimination of negative pressure to the next continuous test;
the test sample was then applied evenly to the face and applied on a daily morning and evening on a daily basis without using any other cosmetic, and the skin was tested for R2, R5, R7 in the 2 week, 4 week, 6 week, 8 week test areas, respectively, for 8 weeks. The test of the same subject is performed by the same measuring person. The skin anti-aging efficacy of the product was evaluated by testing the changes in R2, R5, R7 values of the skin before and after use.
The experimental results are as follows:
results of anti-aging experiments
Note: the closer to 1 the R2, R5, R7, the better the skin elasticity.
As can be seen from the table, after 8 weeks of the experiment, the anti-aging essence creams of the first embodiment, the second embodiment and the second comparative embodiment of the invention have R2, R5 and R7 values greater than 0.900, which shows that the anti-aging essence cream of the invention can increase skin elasticity and resist aging. The addition of 0.1% of recombinant fibronectin-human-like collagen has good anti-aging effect, and the 0.5% of recombinant fibronectin-human-like collagen has the best proportional effect. In addition, the anti-aging essence cream disclosed by the invention is obviously improved in skin elasticity after being used for 6 weeks, and shows that the anti-aging essence cream disclosed by the invention is quick in effect, good in permeability and easy to absorb by skin.
In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (9)
1. The anti-aging composition of the recombinant fibronectin-human-like collagen is characterized by comprising the following raw materials in parts by weight:
1-5 parts of a first emulsifier, 0.1-1 part of a second emulsifier, 1-3 parts of cetostearyl alcohol, 0.5-2 parts of shea butter, 1-5 parts of jojoba seed oil, 1-5 parts of hydrogenated polyisobutene, 1-5 parts of isononyl isononanoate, 1-5 parts of acticore, 0.1-1 part of phytosterol/octyldodecanol lauroyl glutamate, 0.1-1 part of tocopherol acetate, 0.1-1 part of glyceramide ethanol methacrylate/stearyl alcohol methacrylate copolymer, 1-5 parts of polydimethylsiloxane, 1-5 parts of DC-3451-5 parts of water, 100 parts of 1, 3-propylene glycol, 1-10 parts of glycerol, 0.1-2 parts of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.1-1 part of lactobacillus/rice fermentation product, 0.1-0.5 part of allantoin, 0.01-0.2 part of β -dextran, 1-5 parts of trehalose, 0.01-5 parts of polyglutamic acid yeast, 0.01-2 parts of hyaluronic acid, 0.01-352 parts of a recombinant human collagen peptide, 0.1-2 parts of matrine-2 parts of sodium hyaluronate, 0.01-2 parts of a biological hydrolysate and 0.1-1-2 parts of a recombinant polypeptide.
2. The recombinant fibronectin-like human collagen anti-aging composition according to claim 1, wherein the composition comprises 3 to 5 parts of first emulsifier, 0.5 to 1 part of second emulsifier, 2 to 3 parts of cetearyl alcohol, 1 to 2 parts of shea butter, 2 to 5 parts of jojoba seed oil, 2 to 5 parts of hydrogenated polyisobutene, 3 to 5 parts of isononyl isononanoate, 2 to 5 parts of actire, 0.5 to 1 part of phytosterol/octyldodecanol lauroyl glutamate, 0.5 to 1 part of tocopheryl acetate, 0.5 to 1 part of glyceramide ethyl alcohol methacrylate/stearyl alcohol methacrylate copolymer, 1.5 to 5 parts of polydimethylsiloxane, 0.5 to 5 parts of DC-3452 to 5 parts of water, 100 parts of 1,3 to 10 parts of 1, 3-propylene glycol, 3 to 10 parts of glycerol, 0.8 to 2 parts of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.5 to 1 part of lactobacillus/rice fermentation product, 0.15 to 0.15 parts of allantoin, 0.850.83 to 10 parts of yeast, 0.5 to 2 parts of recombinant dextran, 0.05 to 2 parts of dextran sulfate, 0.5 to 2 parts of collagen hydrolysate, 0.5 to 2 parts of sodium alginate, 0.5 to 2 parts of dextran, 0.5 to 2 parts of zymolysate, 0.5 to 2 parts of dextran, 0.5 to 2 parts of sodium alginate, 0.5 to 2 parts of chitosan, and 0.5 to 2 parts of chitosan hydrolysate of chitosan, 0.5 to 2 parts of sodium alginate, 0.5 to 2 parts of chitosan, 0.5 to 2.
3. The recombinant fibronectin-like human collagen anti-aging composition according to claim 1 or 2, wherein the emulsifier comprises the following components in percentage by weight: candelilla wax/jojoba/rice bran polyglycerol-3 esters 30%, glyceryl stearate 30%, cetostearyl alcohol 20%, sodium stearoyl lactylate 20%.
4. The recombinant fibronectin-like human collagen anti-aging composition as claimed in claim 1 or 2, wherein the percentage of the two components of the emulsifier is as follows: 80% of C14-22 alcohol and 20% of C12-20 alkyl glucoside.
5. The recombinant fibronectin-like human collagen anti-aging composition according to claim 1 or 2, wherein the acticore comprises the following components in percentage: 60% of jojoba esters, 4% of cercis chinensis flower wax, 32% of sunflower seed wax and 34% of polyglycerol-34%.
6. The recombinant fibronectin-like human collagen anti-aging composition according to claim 1 or 2, wherein the GC-04 comprises the following components in percentage: 2% of glyceryl caprylate, 13% of caprylyl hydroximic acid, 1% of p-hydroxyacetophenone, 81% of propylene glycol and 3% of water.
7. The anti-aging composition of recombinant fibronectin-like human collagen according to claim 1 or 2, wherein the Matrixyl3000 comprises the following components in percentage: 53.485% of glycerin, 25% of water, 20% of butanediol, 1% of carbomer, 200.5% of polysorbate, 10.01% of palmitoyl tripeptide and 70.005% of palmitoyl tetrapeptide.
8. The recombinant fibronectin-human-like collagen anti-aging composition according to claim 1 or 2, wherein the percentage of each component of the recombinant fibronectin-human-like collagen concentrate is as follows: 50% of fibronectin and 50% of human-like collagen.
9. The method for preparing the recombinant fibronectin-like human collagen anti-aging composition according to claim 1 or 2, wherein the method comprises the following steps:
a) adding emulsifier I, emulsifier II, cetostearyl alcohol, shea butter, jojoba seed oil, hydrogenated polyisobutene, isononyl isononanoate, Acticire, phytosterol/octyldodecanol lauroyl glutamate, tocopherol acetate, glyceramide ethanol methacrylate/stearyl alcohol methacrylate copolymer, polydimethylsiloxane and DC-345 into an oil phase pot at 80-86 ℃ according to a ratio, uniformly stirring, and keeping the temperature for 10 minutes;
b) adding water, 1, 3-propylene glycol, glycerol, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, lactobacillus/rice fermentation product, allantoin, β -glucan, trehalose, sodium polyglutamate and sodium hyaluronate into an emulsifying pot according to a ratio, uniformly stirring, heating the emulsifying pot to 85-95 ℃, and then preserving heat and stirring for 30 minutes;
c) pumping the materials in the oil phase pot into an emulsifying pot, stirring and homogenizing for 5 minutes, keeping the temperature and stirring for 20 minutes continuously, and then cooling to 42 ℃;
d) then putting GC-04, carnosine, the lysate of the yeast fermentation product of the second split yeast, Matrixyl3000, bioglycan-1, acetyl tetrapeptide-2 and the recombinant fibronectin-human-like collagen concentrated solution into an emulsifying pot according to the proportion, stirring for 20 minutes, and cooling to 38 ℃;
e) and finally filtering and discharging.
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| CN113499428A (en) * | 2021-06-25 | 2021-10-15 | 上海淳誉健康科技有限公司 | Cell engineering transdermal nutrition-based fibronectin anti-aging regeneration repair method |
| CN113717290A (en) * | 2021-09-09 | 2021-11-30 | 北京添易医学研究院 | Composite transdermal recombinant fibronectin and application thereof |
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| US20220218584A1 (en) * | 2021-01-13 | 2022-07-14 | Allergan Sales, Llc | Topical composition and method of use |
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| CN113717290A (en) * | 2021-09-09 | 2021-11-30 | 北京添易医学研究院 | Composite transdermal recombinant fibronectin and application thereof |
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| CN114145457A (en) * | 2021-12-10 | 2022-03-08 | 黑龙江飞鹤乳业有限公司 | Hypoglycemic composition |
| CN115089755A (en) * | 2022-07-28 | 2022-09-23 | 浙江美尚洁生物科技有限公司 | Medical dressing composition of recombined fibronectin-humanized collagen with repairing effect and preparation method thereof |
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