Totally-enclosed inverted microscope with multi-zone scanning function
Technical Field
The invention discloses a totally-enclosed inverted microscope with a multi-zone scanning function, and relates to the technical field of microscopy.
Background
An inverted microscope is a microscope with an illumination system above the stage and an objective lens below the stage, and is commonly used to observe cultured living cells. Generally, living cells (e.g., microorganisms, animal cells, etc.) are cultured in a container (e.g., a multi-well plate, a petri dish) containing a culture solution, and the culture container is placed in an incubator. When observing such cells, the culture container is generally taken out of the incubator and placed under an inverted microscope for observation, and the following problems tend to occur: 1. since microorganisms, cells, and the like are in the culture medium, the culture medium vibrates when the cells are taken out of the incubator, and the microorganisms or the cells are difficult to observe and track in their natural movement. 2. The liquid flow easily interferes with the growth of cells, and the in-situ observation of the cells is difficult to realize. If the microscope is directly placed in the incubator for observation, the following problems will be caused: 1. the incubator belongs to a high-temperature and high-humidity environment, is easy to corrode an inverted microscope, influences the service life of the microscope, and is particularly easy to damage due to the fact that the lens of the microscope has high requirements on optical performance. 2. Liquid media easily spills on the stage and further contaminates the microscope.
Disclosure of Invention
In order to solve the problems, the invention discloses a totally-enclosed inverted microscope with a multi-region scanning function, which realizes the in-situ observation of liquid cultures by synchronously moving a light source and an optical lens component and keeping an objective table still; the inverted microscope is positioned in the completely closed box body, and cannot be influenced by the high-temperature and high-humidity environment in the incubator, so that the service life of the inverted microscope is prolonged; the floor area of the invention is greatly reduced by the specially arranged longitudinal moving mechanism; the inverted microscope and the culture medium are isolated, and the microscope pollution caused by the culture solution which is scattered on the objective table is effectively prevented.
In order to meet the technical requirements, the technical scheme of the invention is as follows:
a totally-enclosed inverted microscope with a multi-zone scanning function comprises an inverted microscope body, wherein the inverted microscope body comprises a light source, the light source is connected with an optical lens assembly through a connecting structure, and the light source and the optical lens assembly keep synchronous movement; the light source and the optical lens assembly are connected with a transverse moving structure and a longitudinal moving structure; and a fixedly arranged transparent objective table is arranged between the light source and the optical lens component.
In a further improvement, the transparent object stage is fixed on the box body; the box body comprises a lower box body used for mounting the optical lens assembly, a transverse moving structure and a longitudinal moving structure, the lower box body is communicated with a middle box body used for placing the connecting structure, and the middle box body is communicated with an upper box body used for placing the light source; the lower box body, the middle box body and the upper box body form a closed structure, and the bottom of the upper box body is a transparent plate.
In a further improvement, the transparent plate and the transparent object stage are made of glass or plastic.
In a further improvement, the transverse moving structure and the longitudinal moving structure are both moving platforms driven by cylinders, oil cylinders or motors.
In a further improvement, the connecting structure is a transmission shaft.
In a further improvement, the lower box body is provided with an observation through hole area matched with the optical lens assembly.
In a further improvement, a limiting table with a U-shaped groove is fixed on the outer side of the transparent object stage.
In a further improvement, a container to be observed is placed on the transparent object stage, and the container to be observed is a porous plate.
The advantages of the invention are as follows:
1. the light source and the optical lens component move synchronously, and the objective table is not moved, so that the in-situ observation of the liquid culture is realized.
2. The inverted microscope is arranged in the completely closed box body, and cannot be influenced by a high-temperature and high-humidity environment in the incubator, so that the service life of the inverted microscope is prolonged.
3. The inverted microscope and the culture medium are isolated, and the microscope pollution caused by the culture solution which is scattered on the objective table is effectively prevented.
4. The structure is light and small, and the industrial production is convenient.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic view of the internal structure of the present invention with the case removed.
Fig. 3 is a structure diagram of the lateral movement structure and the longitudinal movement structure of embodiment 2.
Detailed Description
As shown in fig. 1, a totally-enclosed inverted microscope with multi-zone scanning function comprises an inverted microscope body, wherein the inverted microscope body comprises a light source 1, the light source 1 is connected with an optical lens assembly 3 through a connecting structure 2, and the light source 1 and the optical lens assembly 3 keep synchronous movement; the light source 1 and the optical lens assembly 3 are connected with a transverse moving structure and a longitudinal moving structure; and a fixedly arranged transparent object stage 4 is arranged between the light source 1 and the optical lens component 3.
The transparent object stage 4 can be a table top of an n-shaped table, and can also be fixed on the box body 5 as shown in fig. 1; the box body 5 comprises a lower box body 51 for mounting the optical lens assembly 3, a transverse moving structure and a longitudinal moving structure, the lower box body 51 is communicated with a middle box body 52 for placing the connecting structure 2, and the middle box body 52 is communicated with an upper box body 53 for placing the light source 1; the lower case 51, the middle case 52, and the upper case 53 form a closed structure.
The transverse moving structure and the longitudinal moving structure are moving platforms driven by cylinders, oil cylinders or motors.
The connecting structure 2 is a transmission shaft. The box body 51 is formed with a viewing through hole region 18 to be fitted with the optical lens module 3.
A limiting table 19 with a U-shaped groove is fixed on the outer side of the transparent object stage 4.
A container 22 to be observed is placed on the transparent object stage 4, and the container 22 to be observed is a porous plate. The optical lens assembly 3 includes an objective lens. The light source 1 is a phase contrast illumination source.
The inverted microscope is arranged in the closed box body 5, so the inverted microscope is not influenced by moisture and temperature in the incubator and is not easy to corrode, and the objective table does not need to move, but the light source 1 and the optical lens component 3 move together, so that the influence on the culture solution when the observation position is adjusted is avoided, and the in-situ observation is carried out under the condition that the growth and movement environments of microorganisms or cells are not influenced.
Example 2
When the longitudinal moving structures are cylinders, oil cylinders or telescopic motors, the connecting structure 2 can move back and forth inevitably, a middle box body 52 with large width in the front-back direction is needed, the whole box body occupies a large area, the occupied area of the whole device is increased, and the device is not suitable for test bed table tops with tense use areas. In contrast, the following modifications are made on the basis of example 1:
first guide rails 6 are formed on two sides in the lower box body 51, an installation frame 7 is connected onto the first guide rails 6 in a sliding mode, a first screw 8 is connected onto the installation frame 7 in a threaded mode, and the first screw 8 is connected with a first motor 9 to form a transverse moving structure; the mounting frame 7 is connected with the optical lens assembly 3 through a longitudinal moving structure.
The longitudinal moving structure comprises a second screw rod 10 in threaded connection with the optical lens assembly 3, and the second screw rod 10 is connected with a second motor 11; the optical lens component 3 is also connected with a first transmission belt 12, one end of the first transmission belt 12 is sleeved on a first rotating wheel 13 which is coupled with the mounting frame 7 in a shaft way, and the other end of the first transmission belt 12 is sleeved on the connecting structure 2; one end of a second transmission belt 14 is sleeved at the upper end of the connecting structure 2, and the other end of the second transmission belt 14 is sleeved on a second rotating wheel 15; the light source 1 is fixedly connected with the second transmission belt 14 and is connected to a light source slide rail 17 of the light source mounting frame 16 in a sliding manner; second wheel 15 is journalled on a light source mounting bracket 16.
The mounting frame 7 is connected with the first guide rail 6 in a sliding way through a first groove sliding block 20; the light source 1 is connected with the light source slide rail 17 in a sliding way through a second groove slide block 21.
Thus, when the light source 1 and the optical lens assembly 3 need to be adjusted back and forth, the transverse moving structure and the longitudinal moving structure keep synchronous operation, and when the longitudinal moving structure moves longitudinally, the optical lens assembly 3 is driven to move back and forth by the second lead screw 10, the optical lens assembly 3 drives the first transmission belt 12 to rotate, so that the transmission shaft rotates, the second transmission belt 14 is driven to rotate, and the second transmission belt 14 drives the light source 1 to move back and forth. The device converts the front-back displacement of the optical lens component 3 into the rotation of the transmission shaft and then into the front-back displacement of the optical lens component 3, so that the transmission shaft, namely the connecting structure, does not need to move back and forth, the longitudinal length of the box body is greatly reduced, and the front-back left-right adjustment is realized in a limited space.
The foregoing is directed to a specific embodiment of the present invention, which is not intended to be limited to the specific form set forth herein, but is to be accorded the widest scope consistent with the principles and novel features herein disclosed.